AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380016353.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380016353.01.T03 MTR_3g088010 94.301 193 0 2 37 229 1 182 2.59e-128 361
MsG0380016353.01.T01 MTR_3g088010 89.560 182 0 2 1 163 1 182 2.82e-115 325
MsG0380016353.01.T02 MTR_3g088010 99.454 183 0 1 37 219 1 182 1.22e-131 369
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380016353.01.T03 AT1G21830 40.000 195 71 6 44 229 7 164 2.00e-26 101
MsG0380016353.01.T03 AT1G44608 31.868 182 80 5 49 228 12 151 1.39e-13 67.0
MsG0380016353.01.T01 AT1G21830 46.341 164 74 4 8 163 7 164 1.27e-32 115
MsG0380016353.01.T01 AT1G44608 38.065 155 76 4 13 162 12 151 1.28e-17 75.9
MsG0380016353.01.T02 AT1G21830 41.304 184 74 5 44 219 7 164 8.66e-28 105
MsG0380016353.01.T02 AT1G44608 35.838 173 75 7 49 218 12 151 3.91e-14 68.2

Find 45 sgRNAs with CRISPR-Local

Find 149 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACACCAACAACTACTTGTTT+AGG 0.281173 3:-82383638 None:intergenic
ATTCATAAGATCTTTGCTTT+TGG 0.295772 3:+82383827 MsG0380016353.01.T01:CDS
TCAATCAAGTTTGAAGGAAA+TGG 0.311026 3:+82385743 MsG0380016353.01.T01:CDS
GAATGAAAGGCTTCTTCTAT+TGG 0.318441 3:+82383676 MsG0380016353.01.T01:CDS
CACCAACAACTACTTGTTTA+GGG 0.318727 3:-82383637 None:intergenic
CTTTATTAACAAACATGAAT+AGG 0.318817 3:+82383576 MsG0380016353.01.T03:CDS
CAAAGTCACGTGACACATTC+AGG 0.325911 3:+82385873 MsG0380016353.01.T01:CDS
AGCCTTTCATTCAAGCATAA+AGG 0.335003 3:-82383665 None:intergenic
TTATGAATAGAAGAAGAATT+TGG 0.340183 3:-82383812 None:intergenic
ACATGAATAGGAATGGATAT+AGG 0.360762 3:+82383588 MsG0380016353.01.T03:CDS
CAATCAAGTTTGAAGGAAAT+GGG 0.363983 3:+82385744 MsG0380016353.01.T01:CDS
GCCTTTCATTCAAGCATAAA+GGG 0.367664 3:-82383664 None:intergenic
CATGAATAGGAATGGATATA+GGG 0.372518 3:+82383589 MsG0380016353.01.T03:CDS
GGTTCTTCTTGCAATTCTCT+AGG 0.383703 3:-82385811 None:intergenic
TGGTCTCTAATTTGTCCTCA+TGG 0.391766 3:-82385832 None:intergenic
CACCCTAAACAAGTAGTTGT+TGG 0.404809 3:+82383635 MsG0380016353.01.T01:CDS
TTCATATCAATCAAGTTTGA+AGG 0.415927 3:+82385737 MsG0380016353.01.T01:CDS
GCCCTTTATGCTTGAATGAA+AGG 0.420777 3:+82383663 MsG0380016353.01.T01:CDS
GTCACATGTTCCAGCTCATA+AGG 0.443360 3:+82385912 MsG0380016353.01.T01:CDS
TGCATGCTCTATCACACTCT+TGG 0.456919 3:-82385852 None:intergenic
GTTCTTCTTGCAATTCTCTA+GGG 0.470567 3:-82385810 None:intergenic
AAGAAGAACGGTGGTTATGA+TGG 0.478238 3:-82383721 None:intergenic
TGAATAGAAGAAGAATTTGG+TGG 0.500634 3:-82383809 None:intergenic
TTAACAAACATGAATAGGAA+TGG 0.508320 3:+82383581 MsG0380016353.01.T03:CDS
CTTCTATTGGCTGCAAAACA+AGG 0.509299 3:+82383689 MsG0380016353.01.T01:CDS
ATTTGGTGGCTTTGTGTGTA+CGG 0.533418 3:-82383795 None:intergenic
CTTCTTGCAATTCTCTAGGG+AGG 0.538057 3:-82385807 None:intergenic
ACACATTCAGGTGGAGAAAG+AGG 0.548112 3:+82385885 MsG0380016353.01.T01:CDS
CTTGCAATTCTCTAGGGAGG+AGG 0.560179 3:-82385804 None:intergenic
TCTCGTCACTGCAAATCTCA+TGG 0.576256 3:+82383872 MsG0380016353.01.T01:CDS
TAGAAGATGAAGAAGAAGAA+CGG 0.577088 3:-82383733 None:intergenic
TTCAGGTGGAGAAAGAGGAT+TGG 0.577306 3:+82385890 MsG0380016353.01.T01:CDS
GATATCTCTCCTAGTCCAGA+AGG 0.583190 3:+82383911 MsG0380016353.01.T01:CDS
GGAAAAGAGCACGGTCTCAA+AGG 0.587379 3:+82385778 MsG0380016353.01.T01:CDS
CAACAACTACTTGTTTAGGG+TGG 0.614040 3:-82383634 None:intergenic
AGGAAATGGGGTAGCCTCGT+GGG 0.614477 3:+82385757 MsG0380016353.01.T01:CDS
TATTGGCTGCAAAACAAGGT+CGG 0.618913 3:+82383693 MsG0380016353.01.T01:CDS
AAGGAAATGGGGTAGCCTCG+TGG 0.628547 3:+82385756 MsG0380016353.01.T01:CDS
AATCAAGTTTGAAGGAAATG+GGG 0.642471 3:+82385745 MsG0380016353.01.T01:CDS
AGCCTCGTGGGAAAAGAGCA+CGG 0.642699 3:+82385769 MsG0380016353.01.T01:CDS
AAGAACGGTGGTTATGATGG+TGG 0.643533 3:-82383718 None:intergenic
AAGATGAAGAAGAAGAACGG+TGG 0.658130 3:-82383730 None:intergenic
TTAGGGTGGAAATTGCAACA+TGG 0.705580 3:-82383620 None:intergenic
AGTCACGTGACACATTCAGG+TGG 0.713866 3:+82385876 MsG0380016353.01.T01:CDS
GAATTGCAAGAAGAACCATG+AGG 0.821093 3:+82385817 MsG0380016353.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATTATATTTCAAGATTA+TGG + Chr3:82385010-82385029 MsG0380016353.01.T01:intron 10.0%
!! TGTTAGTAATTAAATTAATT+TGG - Chr3:82385532-82385551 None:intergenic 10.0%
!! TTATACTTTATACAATATTA+GGG - Chr3:82385487-82385506 None:intergenic 10.0%
!!! AAATAATAAATAAAAAGTGT+TGG - Chr3:82384629-82384648 None:intergenic 10.0%
!!! AATAATAAATAAAAAGTGTT+GGG - Chr3:82384628-82384647 None:intergenic 10.0%
!! ATAATAATAATGCTCTTAAT+TGG - Chr3:82384982-82385001 None:intergenic 15.0%
!! CAAATTAATTAAAAAGAAGT+TGG + Chr3:82384253-82384272 MsG0380016353.01.T01:intron 15.0%
!! CAATAATGAAAAGAAATTAA+TGG - Chr3:82384372-82384391 None:intergenic 15.0%
!! GTTATACTTTATACAATATT+AGG - Chr3:82385488-82385507 None:intergenic 15.0%
!!! ATAATAAATAAAAAGTGTTG+GGG - Chr3:82384627-82384646 None:intergenic 15.0%
!!! CAACTTCTTTTTAATTAATT+TGG - Chr3:82384255-82384274 None:intergenic 15.0%
!!! CATTAATTTCTTTTCATTAT+TGG + Chr3:82384370-82384389 MsG0380016353.01.T01:intron 15.0%
!!! TTTGTTAATAAAGGTTTTTT+TGG - Chr3:82383569-82383588 None:intergenic 15.0%
!! ATTAATTAAAAAGAAGTTGG+TGG + Chr3:82384256-82384275 MsG0380016353.01.T01:intron 20.0%
!! CTTTATTAACAAACATGAAT+AGG + Chr3:82383576-82383595 MsG0380016353.01.T03:CDS 20.0%
!! TTATGAATAGAAGAAGAATT+TGG - Chr3:82383815-82383834 None:intergenic 20.0%
!!! AGAATTCTTTAATTCTTTTG+CGG + Chr3:82384183-82384202 MsG0380016353.01.T01:intron 20.0%
!!! ATATTAATGATTTTACCTTC+TGG - Chr3:82383929-82383948 None:intergenic 20.0%
!!! ATTATAATTGAAGCATTAGT+TGG + Chr3:82384309-82384328 MsG0380016353.01.T01:intron 20.0%
!!! CTATTCATGTTTGTTAATAA+AGG - Chr3:82383578-82383597 None:intergenic 20.0%
!!! TAATTTCTTTTCATTATTGG+AGG + Chr3:82384373-82384392 MsG0380016353.01.T01:intron 20.0%
!!! TGGAAAAAAAAATCTGTTTT+GGG - Chr3:82384235-82384254 None:intergenic 20.0%
!!! TTGGAAAAAAAAATCTGTTT+TGG - Chr3:82384236-82384255 None:intergenic 20.0%
! AAAGATTAATCTTTCGAATC+AGG - Chr3:82384151-82384170 None:intergenic 25.0%
! AAGAAAGTGAATTGTGAAAA+AGG - Chr3:82383530-82383549 None:intergenic 25.0%
! ACTAGTAAATCAAATGAGAT+AGG + Chr3:82385197-82385216 MsG0380016353.01.T01:intron 25.0%
! ATTAAGAGAGAGAATTTATG+TGG - Chr3:82384553-82384572 None:intergenic 25.0%
! ATTCGAAAGATTAATCTTTG+CGG + Chr3:82384152-82384171 MsG0380016353.01.T01:intron 25.0%
! CAAGTTTGATTGGATAAATA+TGG - Chr3:82384717-82384736 None:intergenic 25.0%
! CATATTTATCCAATCAAACT+TGG + Chr3:82384715-82384734 MsG0380016353.01.T01:intron 25.0%
! TATTTCAAGATTATGGTATG+TGG + Chr3:82385017-82385036 MsG0380016353.01.T01:intron 25.0%
! TTAACAAACATGAATAGGAA+TGG + Chr3:82383581-82383600 MsG0380016353.01.T03:CDS 25.0%
! TTCATATCAATCAAGTTTGA+AGG + Chr3:82385737-82385756 MsG0380016353.01.T01:CDS 25.0%
!! ATTCATAAGATCTTTGCTTT+TGG + Chr3:82383827-82383846 MsG0380016353.01.T01:CDS 25.0%
!! TGATATTTCATGTTTGGTTT+GGG + Chr3:82384332-82384351 MsG0380016353.01.T01:intron 25.0%
!!! CTTGTCTATGTATTTTTACT+TGG + Chr3:82385627-82385646 MsG0380016353.01.T01:intron 25.0%
!!! GCATTGAACTATTTTAATAG+TGG - Chr3:82385117-82385136 None:intergenic 25.0%
AAGATTATGGTATGTGGATT+TGG + Chr3:82385023-82385042 MsG0380016353.01.T01:intron 30.0%
AAGTGTAAGACCATTTACAA+TGG + Chr3:82384477-82384496 MsG0380016353.01.T01:intron 30.0%
AATCAAGTTTGAAGGAAATG+GGG + Chr3:82385745-82385764 MsG0380016353.01.T01:CDS 30.0%
ACATGAATAGGAATGGATAT+AGG + Chr3:82383588-82383607 MsG0380016353.01.T03:CDS 30.0%
ACTAGTAGTAGTAAGACATA+AGG + Chr3:82384885-82384904 MsG0380016353.01.T01:intron 30.0%
AGTAGCTGAGCATAAAAATT+AGG + Chr3:82385444-82385463 MsG0380016353.01.T01:intron 30.0%
ATCTGACATTCAACACAAAA+AGG - Chr3:82384840-82384859 None:intergenic 30.0%
ATTAGGGTACACAACTAATT+TGG + Chr3:82385052-82385071 MsG0380016353.01.T01:intron 30.0%
ATTCTCATTTCTCATTCCAA+GGG - Chr3:82385687-82385706 None:intergenic 30.0%
CAATCAAGTTTGAAGGAAAT+GGG + Chr3:82385744-82385763 MsG0380016353.01.T01:CDS 30.0%
CATGAATAGGAATGGATATA+GGG + Chr3:82383589-82383608 MsG0380016353.01.T03:CDS 30.0%
GAAAAAGGAAAGAAAGAAGA+GGG - Chr3:82383515-82383534 None:intergenic 30.0%
GAGCATTATTATTATGACAC+AGG + Chr3:82384987-82385006 MsG0380016353.01.T01:intron 30.0%
GTGATATTTCATGTTTGGTT+TGG + Chr3:82384331-82384350 MsG0380016353.01.T01:intron 30.0%
TAGAAGATGAAGAAGAAGAA+CGG - Chr3:82383736-82383755 None:intergenic 30.0%
TAGTAGTAAGACATAAGGAT+GGG + Chr3:82384890-82384909 MsG0380016353.01.T01:intron 30.0%
TCAAATTGTATCAAATCCGT+CGG + Chr3:82385409-82385428 MsG0380016353.01.T01:intron 30.0%
TCAATCAAGTTTGAAGGAAA+TGG + Chr3:82385743-82385762 MsG0380016353.01.T01:CDS 30.0%
TGAAAAAGGAAAGAAAGAAG+AGG - Chr3:82383516-82383535 None:intergenic 30.0%
TGAATAGAAGAAGAATTTGG+TGG - Chr3:82383812-82383831 None:intergenic 30.0%
! AGTGTTGAATGAAAAAACCT+TGG - Chr3:82384602-82384621 None:intergenic 30.0%
! TCATTAGATTTTGCTTTGTG+TGG + Chr3:82385655-82385674 MsG0380016353.01.T01:intron 30.0%
!! AAAAAGAAGTTGGTGGAAAA+AGG + Chr3:82384263-82384282 MsG0380016353.01.T01:intron 30.0%
!! AGTTGGTGATATTTCATGTT+TGG + Chr3:82384326-82384345 MsG0380016353.01.T01:intron 30.0%
!! GATATTTCATGTTTGGTTTG+GGG + Chr3:82384333-82384352 MsG0380016353.01.T01:intron 30.0%
!!! TTGTGAACTAGCAACTTTTT+TGG - Chr3:82383762-82383781 None:intergenic 30.0%
AAATATGGAGCTTCAGAACA+CGG - Chr3:82384702-82384721 None:intergenic 35.0%
ACACCAACAACTACTTGTTT+AGG - Chr3:82383641-82383660 None:intergenic 35.0%
AGAGAATTTATGTGGAGTGT+TGG - Chr3:82384545-82384564 None:intergenic 35.0%
AGCCTTTCATTCAAGCATAA+AGG - Chr3:82383668-82383687 None:intergenic 35.0%
ATTCAACTTTAACCTCTCCA+AGG + Chr3:82384582-82384601 MsG0380016353.01.T01:intron 35.0%
CACCAACAACTACTTGTTTA+GGG - Chr3:82383640-82383659 None:intergenic 35.0%
CATTCTCATTTCTCATTCCA+AGG - Chr3:82385688-82385707 None:intergenic 35.0%
GAATCAGGCATGATCATATT+CGG - Chr3:82384136-82384155 None:intergenic 35.0%
GAATGAAAGGCTTCTTCTAT+TGG + Chr3:82383676-82383695 MsG0380016353.01.T01:CDS 35.0%
GCCTTTCATTCAAGCATAAA+GGG - Chr3:82383667-82383686 None:intergenic 35.0%
GTAGTAGTAAGACATAAGGA+TGG + Chr3:82384889-82384908 MsG0380016353.01.T01:intron 35.0%
GTGTATTAGATGCTGACATA+AGG - Chr3:82385370-82385389 None:intergenic 35.0%
GTGTTGGGAATTGAAAAAGT+GGG - Chr3:82384529-82384548 None:intergenic 35.0%
GTTCTTCTTGCAATTCTCTA+GGG - Chr3:82385813-82385832 None:intergenic 35.0%
TACTAACAGTGCAACTAGTT+GGG + Chr3:82385544-82385563 MsG0380016353.01.T01:intron 35.0%
TGAATGAAAAAACCTTGGAG+AGG - Chr3:82384597-82384616 None:intergenic 35.0%
TTACTAACAGTGCAACTAGT+TGG + Chr3:82385543-82385562 MsG0380016353.01.T01:intron 35.0%
! AGTGTTGGGAATTGAAAAAG+TGG - Chr3:82384530-82384549 None:intergenic 35.0%
! ATGATTTTACCTTCTGGACT+AGG - Chr3:82383923-82383942 None:intergenic 35.0%
! GAGAATTTATGTGGAGTGTT+GGG - Chr3:82384544-82384563 None:intergenic 35.0%
! GTTGTGATGATGACGAAATA+AGG + Chr3:82384447-82384466 MsG0380016353.01.T01:intron 35.0%
! TATGGTATGTGGATTTGGTA+GGG + Chr3:82385028-82385047 MsG0380016353.01.T01:intron 35.0%
! TTATGGTATGTGGATTTGGT+AGG + Chr3:82385027-82385046 MsG0380016353.01.T01:intron 35.0%
! TTGGGTCATTGTTTTCTTCT+TGG + Chr3:82384928-82384947 MsG0380016353.01.T01:intron 35.0%
!! ATGTTGGTGACCATTGTAAA+TGG - Chr3:82384490-82384509 None:intergenic 35.0%
!! ATTGAAAAAGTGGGTGTTGA+AGG - Chr3:82384520-82384539 None:intergenic 35.0%
!! TGTTGAAGGGTGTTTAATGT+TGG - Chr3:82384506-82384525 None:intergenic 35.0%
!! TTGAAAAAGTGGGTGTTGAA+GGG - Chr3:82384519-82384538 None:intergenic 35.0%
!!! TGAACTAGCAACTTTTTTGG+AGG - Chr3:82383759-82383778 None:intergenic 35.0%
AACACAAAAAGGCCTGCAAT+GGG - Chr3:82384829-82384848 None:intergenic 40.0%
AAGAAGAACGGTGGTTATGA+TGG - Chr3:82383724-82383743 None:intergenic 40.0%
AAGATGAAGAAGAAGAACGG+TGG - Chr3:82383733-82383752 None:intergenic 40.0%
ACTAACAGTGCAACTAGTTG+GGG + Chr3:82385545-82385564 MsG0380016353.01.T01:intron 40.0%
AGGATGGGAAAGAAAGCTTT+CGG + Chr3:82384905-82384924 MsG0380016353.01.T01:intron 40.0%
ATGTCTTCACGTGGAAGATT+AGG + Chr3:82384425-82384444 MsG0380016353.01.T01:intron 40.0%
CAACAACTACTTGTTTAGGG+TGG - Chr3:82383637-82383656 None:intergenic 40.0%
CACCCTAAACAAGTAGTTGT+TGG + Chr3:82383635-82383654 MsG0380016353.01.T01:CDS 40.0%
CATGTTTGAATGTCTTCACG+TGG + Chr3:82384416-82384435 MsG0380016353.01.T01:intron 40.0%
CATTAAAACACTCCCATTGC+AGG + Chr3:82384814-82384833 MsG0380016353.01.T01:intron 40.0%
GAATTGCAAGAAGAACCATG+AGG + Chr3:82385817-82385836 MsG0380016353.01.T01:CDS 40.0%
GATTAATCTTTGCGGTCTCA+TGG + Chr3:82384160-82384179 MsG0380016353.01.T01:intron 40.0%
GCCCTTTATGCTTGAATGAA+AGG + Chr3:82383663-82383682 MsG0380016353.01.T01:CDS 40.0%
GGTTCTTCTTGCAATTCTCT+AGG - Chr3:82385814-82385833 None:intergenic 40.0%
TCATTTCTCATTCCAAGGGT+AGG - Chr3:82385683-82385702 None:intergenic 40.0%
TTAGGGTGGAAATTGCAACA+TGG - Chr3:82383623-82383642 None:intergenic 40.0%
! ATTCTTTTGCGGTCTCATAG+TGG + Chr3:82384194-82384213 MsG0380016353.01.T01:intron 40.0%
! CTTCTATTGGCTGCAAAACA+AGG + Chr3:82383689-82383708 MsG0380016353.01.T01:CDS 40.0%
! GTGGATTTGGTAGGGAATTA+GGG + Chr3:82385036-82385055 MsG0380016353.01.T01:intron 40.0%
! TATTGGCTGCAAAACAAGGT+CGG + Chr3:82383693-82383712 MsG0380016353.01.T01:CDS 40.0%
! TCATTGTTTTCTTCTTGGCG+TGG + Chr3:82384933-82384952 MsG0380016353.01.T01:intron 40.0%
! TGGTCTCTAATTTGTCCTCA+TGG - Chr3:82385835-82385854 None:intergenic 40.0%
! TGTGGATTTGGTAGGGAATT+AGG + Chr3:82385035-82385054 MsG0380016353.01.T01:intron 40.0%
!! ATTTCATGTTTGGTTTGGGG+AGG + Chr3:82384336-82384355 MsG0380016353.01.T01:intron 40.0%
!! ATTTGGTGGCTTTGTGTGTA+CGG - Chr3:82383798-82383817 None:intergenic 40.0%
AAGAACGGTGGTTATGATGG+TGG - Chr3:82383721-82383740 None:intergenic 45.0%
AAGCATGAACTGGATGAGAG+AGG - Chr3:82384788-82384807 None:intergenic 45.0%
AATGTGCTGCAAGCATGAAC+TGG - Chr3:82384798-82384817 None:intergenic 45.0%
ACACATTCAGGTGGAGAAAG+AGG + Chr3:82385885-82385904 MsG0380016353.01.T01:CDS 45.0%
CAAAGTCACGTGACACATTC+AGG + Chr3:82385873-82385892 MsG0380016353.01.T01:CDS 45.0%
CAACACAAAAAGGCCTGCAA+TGG - Chr3:82384830-82384849 None:intergenic 45.0%
GATATCTCTCCTAGTCCAGA+AGG + Chr3:82383911-82383930 MsG0380016353.01.T01:CDS 45.0%
GGGAAAGAAAGCTTTCGGTT+GGG + Chr3:82384910-82384929 MsG0380016353.01.T01:intron 45.0%
GTCACATGTTCCAGCTCATA+AGG + Chr3:82385912-82385931 MsG0380016353.01.T01:CDS 45.0%
TACTAATACCATGCTGCCGA+CGG - Chr3:82385428-82385447 None:intergenic 45.0%
TCTCGTCACTGCAAATCTCA+TGG + Chr3:82383872-82383891 MsG0380016353.01.T01:CDS 45.0%
TGCATGCTCTATCACACTCT+TGG - Chr3:82385855-82385874 None:intergenic 45.0%
TGGGAAAGAAAGCTTTCGGT+TGG + Chr3:82384909-82384928 MsG0380016353.01.T01:intron 45.0%
TTCAGGTGGAGAAAGAGGAT+TGG + Chr3:82385890-82385909 MsG0380016353.01.T01:CDS 45.0%
! AGAACACGGCAGAGTGTTTA+AGG - Chr3:82384688-82384707 None:intergenic 45.0%
! CTTCTTGCAATTCTCTAGGG+AGG - Chr3:82385810-82385829 None:intergenic 45.0%
!! GGATGAGAGAGGTGTTGAAT+CGG - Chr3:82384777-82384796 None:intergenic 45.0%
!! GTTTGGTTTGGGGAGGAATT+TGG + Chr3:82384343-82384362 MsG0380016353.01.T01:intron 45.0%
!! TTAAGGTGCTGCTTGCTGAT+TGG - Chr3:82384671-82384690 None:intergenic 45.0%
!!! ATAATTTTAATTTATACTTT+TGG - Chr3:82385599-82385618 None:intergenic 5.0%
AGTCACGTGACACATTCAGG+TGG + Chr3:82385876-82385895 MsG0380016353.01.T01:CDS 50.0%
CTTTGTGTGGAACCTACCCT+TGG + Chr3:82385668-82385687 MsG0380016353.01.T01:intron 50.0%
TATCAAATCCGTCGGCAGCA+TGG + Chr3:82385417-82385436 MsG0380016353.01.T01:intron 50.0%
TCCAATCAAACTTGGACGCG+TGG + Chr3:82384723-82384742 MsG0380016353.01.T01:intron 50.0%
! CTTGCAATTCTCTAGGGAGG+AGG - Chr3:82385807-82385826 None:intergenic 50.0%
! GGAAAAGAGCACGGTCTCAA+AGG + Chr3:82385778-82385797 MsG0380016353.01.T01:CDS 50.0%
AAGGAAATGGGGTAGCCTCG+TGG + Chr3:82385756-82385775 MsG0380016353.01.T01:CDS 55.0%
ACTTGGACGCGTGGCAATTC+TGG + Chr3:82384732-82384751 MsG0380016353.01.T01:intron 55.0%
AGCCTCGTGGGAAAAGAGCA+CGG + Chr3:82385769-82385788 MsG0380016353.01.T01:CDS 55.0%
AGGAAATGGGGTAGCCTCGT+GGG + Chr3:82385757-82385776 MsG0380016353.01.T01:CDS 55.0%
GCCACGCGTCCAAGTTTGAT+TGG - Chr3:82384727-82384746 None:intergenic 55.0%
! GACCGTGCTCTTTTCCCACG+AGG - Chr3:82385774-82385793 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
Chr3 gene 82383482 82385937 82383482 ID=MsG0380016353.01;Name=MsG0380016353.01
Chr3 mRNA 82383590 82385937 82383590 ID=MsG0380016353.01.T01;Parent=MsG0380016353.01;Name=MsG0380016353.01.T01;_AED=0.40;_eAED=0.40;_QI=0|0|0|1|0|0|3|0|163
Chr3 exon 82383590 82383745 82383590 ID=MsG0380016353.01.T01:exon:33094;Parent=MsG0380016353.01.T01
Chr3 exon 82383803 82383932 82383803 ID=MsG0380016353.01.T01:exon:33095;Parent=MsG0380016353.01.T01
Chr3 exon 82385732 82385937 82385732 ID=MsG0380016353.01.T01:exon:33096;Parent=MsG0380016353.01.T01
Chr3 CDS 82383590 82383745 82383590 ID=MsG0380016353.01.T01:cds;Parent=MsG0380016353.01.T01
Chr3 CDS 82383803 82383932 82383803 ID=MsG0380016353.01.T01:cds;Parent=MsG0380016353.01.T01
Chr3 CDS 82385732 82385937 82385732 ID=MsG0380016353.01.T01:cds;Parent=MsG0380016353.01.T01
Chr3 mRNA 82383482 82385937 82383482 ID=MsG0380016353.01.T02;Parent=MsG0380016353.01;Name=MsG0380016353.01.T02;_AED=0.39;_eAED=0.39;_QI=0|1|0.5|1|0|0.5|2|0|219
Chr3 exon 82385729 82385937 82385729 ID=MsG0380016353.01.T02:exon:33098;Parent=MsG0380016353.01.T02
Chr3 CDS 82383482 82383932 82383482 ID=MsG0380016353.01.T02:cds;Parent=MsG0380016353.01.T02
Chr3 CDS 82385729 82385937 82385729 ID=MsG0380016353.01.T02:cds;Parent=MsG0380016353.01.T02
Chr3 mRNA 82383482 82385937 82383482 ID=MsG0380016353.01.T03;Parent=MsG0380016353.01;Name=MsG0380016353.01.T03;_AED=0.42;_eAED=0.42;_QI=0|0|0|1|0|0.5|2|0|229
Chr3 exon 82385699 82385937 82385699 ID=MsG0380016353.01.T03:exon:33099;Parent=MsG0380016353.01.T03
Chr3 CDS 82383482 82383932 82383482 ID=MsG0380016353.01.T03:cds;Parent=MsG0380016353.01.T03
Chr3 CDS 82385699 82385937 82385699 ID=MsG0380016353.01.T03:cds;Parent=MsG0380016353.01.T03
Chr3 mRNA 82383678 82383956 82383678 ID=MsG0380016353.01.T04;Parent=MsG0380016353.01;Name=MsG0380016353.01.T04;_AED=0.47;_eAED=0.47;_QI=0|-1|0|1|-1|0|1|0|92
Chr3 exon 82383678 82383956 82383678 ID=MsG0380016353.01.T04:exon:33100;Parent=MsG0380016353.01.T04
Chr3 CDS 82383678 82383956 82383678 ID=MsG0380016353.01.T04:cds;Parent=MsG0380016353.01.T04
Gene Sequence

>MsG0380016353.01.T03

CATCACTTTTGTTTAATTAATTTTAAACTTCCCTCTTCTTTCTTTCCTTTTTCACAATTCACTTTCTTCATTCTTCATAGTGTACCAAAAAAACCTTTATTAACAAACATGAATAGGAATGGATATAGGGATGAAAAACCATGTTGCAATTTCCACCCTAAACAAGTAGTTGTTGGTGTATGCCCTTTATGCTTGAATGAAAGGCTTCTTCTATTGGCTGCAAAACAAGGTCGGCGCCACCATCATAACCACCGTTCTTCTTCTTCATCTTCTACCTCCAAAAAAGTTGCTAGTTCACAAAAGCTTCAAAGTTCCGTACACACAAAGCCACCAAATTCTTCTTCTATTCATAAGATCTTTGCTTTTGGTTCTCTCTTCACTCGTCTTGAATCTCGTCACTGCAAATCTCATGGCAACTTTCACTATGATGATATCTCTCCTAGTCCAGAAGATGAGAATGACTTTAATGTAACTTTTACAGAAGACTCATTCATATCAATCAAGTTTGAAGGAAATGGGGTAGCCTCGTGGGAAAAGAGCACGGTCTCAAAGGTGTCCTCCTCCCTAGAGAATTGCAAGAAGAACCATGAGGACAAATTAGAGACCAAGAGTGTGATAGAGCATGCAAAGTCACGTGACACATTCAGGTGGAGAAAGAGGATTGGTCACATGTTCCAGCTCATAAGGTGA

>MsG0380016353.01.T01

ATGAATAGGAATGGATATAGGGATGAAAAACCATGTTGCAATTTCCACCCTAAACAAGTAGTTGTTGGTGTATGCCCTTTATGCTTGAATGAAAGGCTTCTTCTATTGGCTGCAAAACAAGGTCGGCGCCACCATCATAACCACCGTTCTTCTTCTACAAAGCCACCAAATTCTTCTTCTATTCATAAGATCTTTGCTTTTGGTTCTCTCTTCACTCGTCTTGAATCTCGTCACTGCAAATCTCATGGCAACTTTCACTATGATGATATCTCTCCTAGTCCAGAAGACTCATTCATATCAATCAAGTTTGAAGGAAATGGGGTAGCCTCGTGGGAAAAGAGCACGGTCTCAAAGGTGTCCTCCTCCCTAGAGAATTGCAAGAAGAACCATGAGGACAAATTAGAGACCAAGAGTGTGATAGAGCATGCAAAGTCACGTGACACATTCAGGTGGAGAAAGAGGATTGGTCACATGTTCCAGCTCATAAGGTGA

>MsG0380016353.01.T04

ATGAAAGGCTTCTTCTATTGGCTGCAAAACAAGGTCGGCGCCACCATCATAACCACCGTTCTTCTTCTTCATCTTCTACCTCCAAAAAAGTTGCTAGTTCACAAAAGCTTCAAAGTTCCGTACACACAAAGCCACCAAATTCTTCTTCTATTCATAAGATCTTTGCTTTTGGTTCTCTCTTCACTCGTCTTGAATCTCGTCACTGCAAATCTCATGGCAACTTTCACTATGATGATATCTCTCCTAGTCCAGAAGGTAAAATCATTAATATTAATATAA

>MsG0380016353.01.T02

CATCACTTTTGTTTAATTAATTTTAAACTTCCCTCTTCTTTCTTTCCTTTTTCACAATTCACTTTCTTCATTCTTCATAGTGTACCAAAAAAACCTTTATTAACAAACATGAATAGGAATGGATATAGGGATGAAAAACCATGTTGCAATTTCCACCCTAAACAAGTAGTTGTTGGTGTATGCCCTTTATGCTTGAATGAAAGGCTTCTTCTATTGGCTGCAAAACAAGGTCGGCGCCACCATCATAACCACCGTTCTTCTTCTTCATCTTCTACCTCCAAAAAAGTTGCTAGTTCACAAAAGCTTCAAAGTTCCGTACACACAAAGCCACCAAATTCTTCTTCTATTCATAAGATCTTTGCTTTTGGTTCTCTCTTCACTCGTCTTGAATCTCGTCACTGCAAATCTCATGGCAACTTTCACTATGATGATATCTCTCCTAGTCCAGAAGAAGACTCATTCATATCAATCAAGTTTGAAGGAAATGGGGTAGCCTCGTGGGAAAAGAGCACGGTCTCAAAGGTGTCCTCCTCCCTAGAGAATTGCAAGAAGAACCATGAGGACAAATTAGAGACCAAGAGTGTGATAGAGCATGCAAAGTCACGTGACACATTCAGGTGGAGAAAGAGGATTGGTCACATGTTCCAGCTCATAAGGTGA
Protein sequence

>MsG0380016353.01.T03

HHFCLINFKLPSSFFPFSQFTFFILHSVPKKPLLTNMNRNGYRDEKPCCNFHPKQVVVGVCPLCLNERLLLLAAKQGRRHHHNHRSSSSSSTSKKVASSQKLQSSVHTKPPNSSSIHKIFAFGSLFTRLESRHCKSHGNFHYDDISPSPEDENDFNVTFTEDSFISIKFEGNGVASWEKSTVSKVSSSLENCKKNHEDKLETKSVIEHAKSRDTFRWRKRIGHMFQLIR*

>MsG0380016353.01.T01

MNRNGYRDEKPCCNFHPKQVVVGVCPLCLNERLLLLAAKQGRRHHHNHRSSSTKPPNSSSIHKIFAFGSLFTRLESRHCKSHGNFHYDDISPSPEDSFISIKFEGNGVASWEKSTVSKVSSSLENCKKNHEDKLETKSVIEHAKSRDTFRWRKRIGHMFQLIR*

>MsG0380016353.01.T04

MKGFFYWLQNKVGATIITTVLLLHLLPPKKLLVHKSFKVPYTQSHQILLLFIRSLLLVLSSLVLNLVTANLMATFTMMISLLVQKVKSLILI*

>MsG0380016353.01.T02

HHFCLINFKLPSSFFPFSQFTFFILHSVPKKPLLTNMNRNGYRDEKPCCNFHPKQVVVGVCPLCLNERLLLLAAKQGRRHHHNHRSSSSSSTSKKVASSQKLQSSVHTKPPNSSSIHKIFAFGSLFTRLESRHCKSHGNFHYDDISPSPEEDSFISIKFEGNGVASWEKSTVSKVSSSLENCKKNHEDKLETKSVIEHAKSRDTFRWRKRIGHMFQLIR*