AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480022178.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480022178.01.T01 MTR_4g083970 79.795 292 13 2 1 248 1 290 2.84e-156 436
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480022178.01.T01 AT4G19840 41.176 204 69 6 94 249 46 246 1.65e-42 145
MsG0480022178.01.T01 AT4G19850 35.714 210 76 7 94 248 13 218 1.85e-31 115
MsG0480022178.01.T01 AT4G19850 34.568 162 57 3 136 248 15 176 2.56e-25 98.6
MsG0480022178.01.T01 AT1G33920 30.921 152 57 2 145 248 13 164 4.15e-21 87.4
MsG0480022178.01.T01 AT1G65390 28.485 165 70 3 132 248 246 410 8.93e-19 85.1
MsG0480022178.01.T01 AT5G24560 26.000 200 122 8 53 249 17 193 6.65e-15 71.2

Find 57 sgRNAs with CRISPR-Local

Find 94 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTATCCATTTCCAATAATT+TGG 0.178400 4:+69322077 None:intergenic
TGATCGTAATTCTTCCTTAT+TGG 0.201571 4:+69322628 None:intergenic
AACGTTGTTTGTAGAAATAT+TGG 0.268294 4:-69322172 MsG0480022178.01.T01:intron
GGGCAGAAGATCCAAATTAT+TGG 0.313201 4:-69322088 MsG0480022178.01.T01:CDS
GCAACACAAGGAAAATTTAA+TGG 0.313866 4:-69321376 MsG0480022178.01.T01:CDS
AATGTTCGACTTGTCCTTCC+AGG 0.347328 4:-69321411 MsG0480022178.01.T01:CDS
AAGATCCAAATTATTGGAAA+TGG 0.355234 4:-69322082 MsG0480022178.01.T01:CDS
ACAAAATGAACCAACCAATA+AGG 0.364806 4:-69322642 MsG0480022178.01.T01:CDS
CAGCTCAGGTTGGGAACTTC+CGG 0.369538 4:-69321436 MsG0480022178.01.T01:CDS
TCCCTTGAAGATTTATCAAC+CGG 0.377577 4:+69322422 None:intergenic
TGTTGCTGCTTCTTTCCTCC+TGG 0.398539 4:+69321393 None:intergenic
GGTTGCTGATTGAGTCTCAT+TGG 0.400764 4:+69322511 None:intergenic
CAACATGAAACAGTTGGCTT+TGG 0.410721 4:+69322136 None:intergenic
ATATAGCATATGAAGTGAAA+TGG 0.426540 4:-69322759 MsG0480022178.01.T01:exon
TAGTTCTTTGATCAAAGGTT+TGG 0.427713 4:+69322490 None:intergenic
CGTAATTCTTCCTTATTGGT+TGG 0.455287 4:+69322632 None:intergenic
GATGCTACAAACTCACCAAC+TGG 0.464067 4:+69321324 None:intergenic
ACGTTGTTTGTAGAAATATT+GGG 0.469595 4:-69322171 MsG0480022178.01.T01:intron
GTTTGTTGTGGCTGTGATTG+TGG 0.475397 4:+69322707 None:intergenic
TATAGCATATGAAGTGAAAT+GGG 0.476453 4:-69322758 MsG0480022178.01.T01:exon
TGCATACAACATGAAACAGT+TGG 0.479048 4:+69322130 None:intergenic
GCTGCTTCTTTCCTCCTGGA+AGG 0.481544 4:+69321397 None:intergenic
AAACTTAGTTCTTTGATCAA+AGG 0.483857 4:+69322485 None:intergenic
CACCGGTTGATAAATCTTCA+AGG 0.484431 4:-69322424 MsG0480022178.01.T01:CDS
TGTTGGTGTTGGTGTTGTTG+TGG 0.488043 4:+69322662 None:intergenic
CAAAAGCACTCTCAATCACC+TGG 0.496488 4:-69322109 MsG0480022178.01.T01:CDS
TAACTATGAGTACATATTGA+AGG 0.507376 4:-69322456 MsG0480022178.01.T01:CDS
GTTAAGAGCACGGTGGATAG+AGG 0.513931 4:-69321349 MsG0480022178.01.T01:CDS
TTGTACTGCCTTCTTGCTGG+AGG 0.519711 4:+69322553 None:intergenic
TAAAAGATCCAGCTCAGGTT+GGG 0.522154 4:-69321445 MsG0480022178.01.T01:intron
GTTCTTACATCATAAGACTA+AGG 0.532133 4:-69322369 MsG0480022178.01.T01:intron
TGTACTGCCTTCTTGCTGGA+GGG 0.535237 4:+69322554 None:intergenic
ATTACGATCACCTTCGAGAA+GGG 0.539166 4:-69322615 MsG0480022178.01.T01:CDS
GAATGTTGATGTGTTTGTTG+TGG 0.547811 4:+69322695 None:intergenic
ATATCAATGTTTGAATATGA+AGG 0.554106 4:-69321276 MsG0480022178.01.T01:CDS
AATATGTACTCATAGTTATG+TGG 0.557085 4:+69322461 None:intergenic
ACCGGTTGATAAATCTTCAA+GGG 0.558433 4:-69322423 MsG0480022178.01.T01:CDS
AGGACAAGTCGAACATTCAC+CGG 0.560100 4:+69321417 None:intergenic
TTGAATATGAAGGTGGTATG+TGG 0.566199 4:-69321266 MsG0480022178.01.T01:CDS
ATTGAAGGATGCAGATTCAC+CGG 0.568231 4:-69322441 MsG0480022178.01.T01:CDS
ATGTGTGATCAGCTATATGC+TGG 0.569259 4:-69322395 MsG0480022178.01.T01:CDS
ATAATTTGGATCTTCTGCCC+AGG 0.570223 4:+69322091 None:intergenic
AGGAAAGAAGCAGCAACACA+AGG 0.570643 4:-69321388 MsG0480022178.01.T01:CDS
TGTTGTGGCTGTGATTGTGG+TGG 0.571307 4:+69322710 None:intergenic
AAAATTACCCTCCAGCAAGA+AGG 0.582340 4:-69322561 MsG0480022178.01.T01:CDS
AATTACGATCACCTTCGAGA+AGG 0.585667 4:-69322616 MsG0480022178.01.T01:CDS
AAACAAGGGCTTGTCATCAA+AGG 0.587551 4:-69321243 MsG0480022178.01.T01:CDS
GAAGGTGGTATGTGGAAACA+AGG 0.592943 4:-69321258 MsG0480022178.01.T01:CDS
AGGCATTGCTATCAAACCAA+AGG 0.606104 4:-69321223 MsG0480022178.01.T01:CDS
TAATGGAAAAGTTAAGAGCA+CGG 0.611140 4:-69321359 MsG0480022178.01.T01:CDS
TTAAAAGATCCAGCTCAGGT+TGG 0.614325 4:-69321446 MsG0480022178.01.T01:intron
TCAATGTTTGAATATGAAGG+TGG 0.618732 4:-69321273 MsG0480022178.01.T01:CDS
AAGGTGGTATGTGGAAACAA+GGG 0.657478 4:-69321257 MsG0480022178.01.T01:CDS
CGGTGGATAGAGGTTCCAGT+TGG 0.671489 4:-69321339 MsG0480022178.01.T01:CDS
AAAAGCACTCTCAATCACCT+GGG 0.675178 4:-69322108 MsG0480022178.01.T01:CDS
GTTCGACTTGTCCTTCCAGG+AGG 0.692090 4:-69321408 MsG0480022178.01.T01:CDS
TGGAAAAGTTAAGAGCACGG+TGG 0.775430 4:-69321356 MsG0480022178.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CTTAAAAAATTATATAGTTT+AGG + Chr4:69322150-69322169 None:intergenic 10.0%
!! AATAACTAAATTCAATTTCA+AGG + Chr4:69321722-69321741 None:intergenic 15.0%
!!! TTTTTCTAAGTTATATGTAA+AGG + Chr4:69322072-69322091 None:intergenic 15.0%
!! AATATGTATGTGTCATATTA+TGG + Chr4:69322354-69322373 None:intergenic 20.0%
!! ATATCAATGTTTGAATATGA+AGG - Chr4:69322701-69322720 MsG0480022178.01.T01:CDS 20.0%
!! GGATTAATTGTTGATATAAT+AGG + Chr4:69321968-69321987 None:intergenic 20.0%
!! TAGGAAAAGATAATGATATA+AGG + Chr4:69321949-69321968 None:intergenic 20.0%
!! TGAAATTATTTGTAATCACT+GGG + Chr4:69321989-69322008 None:intergenic 20.0%
!! TTGAAATTATTTGTAATCAC+TGG + Chr4:69321990-69322009 None:intergenic 20.0%
! AACGTTGTTTGTAGAAATAT+TGG - Chr4:69321805-69321824 MsG0480022178.01.T01:intron 25.0%
! AACTAAATTCAATTTCAAGG+AGG + Chr4:69321719-69321738 None:intergenic 25.0%
! AAGATCCAAATTATTGGAAA+TGG - Chr4:69321895-69321914 MsG0480022178.01.T01:intron 25.0%
! AATATGTACTCATAGTTATG+TGG + Chr4:69321519-69321538 None:intergenic 25.0%
! ACGTTGTTTGTAGAAATATT+GGG - Chr4:69321806-69321825 MsG0480022178.01.T01:intron 25.0%
! ATATAGCATATGAAGTGAAA+TGG - Chr4:69321218-69321237 MsG0480022178.01.T01:CDS 25.0%
! GAAACTTGATATAAAATTCC+TGG + Chr4:69322498-69322517 None:intergenic 25.0%
! TAACTATGAGTACATATTGA+AGG - Chr4:69321521-69321540 MsG0480022178.01.T01:intron 25.0%
! TATAGCATATGAAGTGAAAT+GGG - Chr4:69321219-69321238 MsG0480022178.01.T01:CDS 25.0%
! TGTAATTCATAGATGTTTCT+TGG + Chr4:69322188-69322207 None:intergenic 25.0%
! TGTATCCATTTCCAATAATT+TGG + Chr4:69321903-69321922 None:intergenic 25.0%
!! AAACTTAGTTCTTTGATCAA+AGG + Chr4:69321495-69321514 None:intergenic 25.0%
ACAAAATGAACCAACCAATA+AGG - Chr4:69321335-69321354 MsG0480022178.01.T01:CDS 30.0%
ACAAGTAGCTTGATCTAATT+AGG + Chr4:69322287-69322306 None:intergenic 30.0%
CTTATATTGAGTATGTTTGC+AGG - Chr4:69322393-69322412 MsG0480022178.01.T01:CDS 30.0%
GCAACACAAGGAAAATTTAA+TGG - Chr4:69322601-69322620 MsG0480022178.01.T01:CDS 30.0%
GTTCTTACATCATAAGACTA+AGG - Chr4:69321608-69321627 MsG0480022178.01.T01:intron 30.0%
TAATGGAAAAGTTAAGAGCA+CGG - Chr4:69322618-69322637 MsG0480022178.01.T01:CDS 30.0%
TAGTTCTTTGATCAAAGGTT+TGG + Chr4:69321490-69321509 None:intergenic 30.0%
TCAATGTTTGAATATGAAGG+TGG - Chr4:69322704-69322723 MsG0480022178.01.T01:CDS 30.0%
TGATCGTAATTCTTCCTTAT+TGG + Chr4:69321352-69321371 None:intergenic 30.0%
TTTAGGGACTAAATTGAAGA+TGG + Chr4:69322133-69322152 None:intergenic 30.0%
TTTGTAGCATCTGAAAAAGA+TGG - Chr4:69322665-69322684 MsG0480022178.01.T01:CDS 30.0%
! ATTCTATCTAGACTTTTGTC+TGG - Chr4:69322213-69322232 MsG0480022178.01.T01:intron 30.0%
! TGTTGCTAGCAAATCATTTT+AGG - Chr4:69322304-69322323 MsG0480022178.01.T01:intron 30.0%
!! GGTAATTTTGTTTGATGTTG+AGG + Chr4:69321405-69321424 None:intergenic 30.0%
!! TATTGGTTGGTTCATTTTGT+TGG + Chr4:69321335-69321354 None:intergenic 30.0%
AATGTTAAAAGATCCAGCTC+AGG - Chr4:69322527-69322546 MsG0480022178.01.T01:CDS 35.0%
ATATTGAGTATGTTTGCAGG+AGG - Chr4:69322396-69322415 MsG0480022178.01.T01:CDS 35.0%
CGTAATTCTTCCTTATTGGT+TGG + Chr4:69321348-69321367 None:intergenic 35.0%
GGATACAGCAAAAAGATGTT+AGG - Chr4:69321916-69321935 MsG0480022178.01.T01:intron 35.0%
TATTGAGTATGTTTGCAGGA+GGG - Chr4:69322397-69322416 MsG0480022178.01.T01:CDS 35.0%
TCAAGGAGGACAATTGTAAA+TGG + Chr4:69321705-69321724 None:intergenic 35.0%
TCCCTTGAAGATTTATCAAC+CGG + Chr4:69321558-69321577 None:intergenic 35.0%
TGCATACAACATGAAACAGT+TGG + Chr4:69321850-69321869 None:intergenic 35.0%
TTCATGGAAAATTCGACACA+AGG - Chr4:69322463-69322482 MsG0480022178.01.T01:CDS 35.0%
TTGAATATGAAGGTGGTATG+TGG - Chr4:69322711-69322730 MsG0480022178.01.T01:CDS 35.0%
! GAATGTTGATGTGTTTGTTG+TGG + Chr4:69321285-69321304 None:intergenic 35.0%
! TTGGTTCATTTTGTTGGTGT+TGG + Chr4:69321329-69321348 None:intergenic 35.0%
!! ACCGGTTGATAAATCTTCAA+GGG - Chr4:69321554-69321573 MsG0480022178.01.T01:intron 35.0%
AAAATTACCCTCCAGCAAGA+AGG - Chr4:69321416-69321435 MsG0480022178.01.T01:CDS 40.0%
AAACAAGGGCTTGTCATCAA+AGG - Chr4:69322734-69322753 MsG0480022178.01.T01:CDS 40.0%
AAGGTGGTATGTGGAAACAA+GGG - Chr4:69322720-69322739 MsG0480022178.01.T01:CDS 40.0%
AATTACGATCACCTTCGAGA+AGG - Chr4:69321361-69321380 MsG0480022178.01.T01:CDS 40.0%
ATAATTTGGATCTTCTGCCC+AGG + Chr4:69321889-69321908 None:intergenic 40.0%
ATGTGTGATCAGCTATATGC+TGG - Chr4:69321582-69321601 MsG0480022178.01.T01:intron 40.0%
ATTACGATCACCTTCGAGAA+GGG - Chr4:69321362-69321381 MsG0480022178.01.T01:CDS 40.0%
ATTGAAGGATGCAGATTCAC+CGG - Chr4:69321536-69321555 MsG0480022178.01.T01:intron 40.0%
GGGCAGAAGATCCAAATTAT+TGG - Chr4:69321889-69321908 MsG0480022178.01.T01:intron 40.0%
GTAGCATCTGAAAAAGATGG+CGG - Chr4:69322668-69322687 MsG0480022178.01.T01:CDS 40.0%
TAAAAGATCCAGCTCAGGTT+GGG - Chr4:69322532-69322551 MsG0480022178.01.T01:CDS 40.0%
TTAAAAGATCCAGCTCAGGT+TGG - Chr4:69322531-69322550 MsG0480022178.01.T01:CDS 40.0%
! AACAGATTTTCCCTTCTCGA+AGG + Chr4:69321375-69321394 None:intergenic 40.0%
! AGGCATTGCTATCAAACCAA+AGG - Chr4:69322754-69322773 MsG0480022178.01.T01:exon 40.0%
! CAACATGAAACAGTTGGCTT+TGG + Chr4:69321844-69321863 None:intergenic 40.0%
!! AAAAGCACTCTCAATCACCT+GGG - Chr4:69321869-69321888 MsG0480022178.01.T01:intron 40.0%
!! CACCGGTTGATAAATCTTCA+AGG - Chr4:69321553-69321572 MsG0480022178.01.T01:intron 40.0%
!! GTGTGTTGGTTAGAAGTTCA+TGG - Chr4:69322447-69322466 MsG0480022178.01.T01:CDS 40.0%
AGGAAAGAAGCAGCAACACA+AGG - Chr4:69322589-69322608 MsG0480022178.01.T01:CDS 45.0%
AGGACAAGTCGAACATTCAC+CGG + Chr4:69322563-69322582 None:intergenic 45.0%
GAAGGTGGTATGTGGAAACA+AGG - Chr4:69322719-69322738 MsG0480022178.01.T01:CDS 45.0%
GATGCTACAAACTCACCAAC+TGG + Chr4:69322656-69322675 None:intergenic 45.0%
! AATGTTCGACTTGTCCTTCC+AGG - Chr4:69322566-69322585 MsG0480022178.01.T01:CDS 45.0%
! CTGAACTGAAGAGAGTGTGT+TGG - Chr4:69322433-69322452 MsG0480022178.01.T01:CDS 45.0%
! GGTTGCTGATTGAGTCTCAT+TGG + Chr4:69321469-69321488 None:intergenic 45.0%
! GTTTTGTACTGCCTTCTTGC+TGG + Chr4:69321430-69321449 None:intergenic 45.0%
! TGAAAAAGATGGCGGTGAGA+TGG - Chr4:69322676-69322695 MsG0480022178.01.T01:CDS 45.0%
! TGGAAAAGTTAAGAGCACGG+TGG - Chr4:69322621-69322640 MsG0480022178.01.T01:CDS 45.0%
!! CAAAAGCACTCTCAATCACC+TGG - Chr4:69321868-69321887 MsG0480022178.01.T01:intron 45.0%
!! GTTTGTTGTGGCTGTGATTG+TGG + Chr4:69321273-69321292 None:intergenic 45.0%
!! TGTTGGTGTTGGTGTTGTTG+TGG + Chr4:69321318-69321337 None:intergenic 45.0%
!! TTAAAAAATTATATAGTTTA+GGG + Chr4:69322149-69322168 None:intergenic 5.0%
GACACAAGGAAGCTTTCACC+AGG - Chr4:69322477-69322496 MsG0480022178.01.T01:CDS 50.0%
TGTACTGCCTTCTTGCTGGA+GGG + Chr4:69321426-69321445 None:intergenic 50.0%
TGTTGTGGCTGTGATTGTGG+TGG + Chr4:69321270-69321289 None:intergenic 50.0%
TTGTACTGCCTTCTTGCTGG+AGG + Chr4:69321427-69321446 None:intergenic 50.0%
! GTTAAGAGCACGGTGGATAG+AGG - Chr4:69322628-69322647 MsG0480022178.01.T01:CDS 50.0%
! TGTTGCTGCTTCTTTCCTCC+TGG + Chr4:69322587-69322606 None:intergenic 50.0%
CGGTGGATAGAGGTTCCAGT+TGG - Chr4:69322638-69322657 MsG0480022178.01.T01:CDS 55.0%
GCTGCTTCTTTCCTCCTGGA+AGG + Chr4:69322583-69322602 None:intergenic 55.0%
GTTTGCAGGAGGGATGACTG+AGG - Chr4:69322407-69322426 MsG0480022178.01.T01:CDS 55.0%
! GTTCGACTTGTCCTTCCAGG+AGG - Chr4:69322569-69322588 MsG0480022178.01.T01:CDS 55.0%
!! CAGCTCAGGTTGGGAACTTC+CGG - Chr4:69322541-69322560 MsG0480022178.01.T01:CDS 55.0%
CGGAAGTTCCCAACCTGAGC+TGG + Chr4:69322543-69322562 None:intergenic 60.0%
TGCAGGAGGGATGACTGAGG+TGG - Chr4:69322410-69322429 MsG0480022178.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr4 gene 69321218 69322781 69321218 ID=MsG0480022178.01;Name=MsG0480022178.01
Chr4 mRNA 69321218 69322781 69321218 ID=MsG0480022178.01.T01;Parent=MsG0480022178.01;Name=MsG0480022178.01.T01;_AED=0.49;_eAED=0.50;_QI=19|0.5|0.66|1|1|1|3|0|249
Chr4 exon 69321218 69321455 69321218 ID=MsG0480022178.01.T01:exon:55534;Parent=MsG0480022178.01.T01
Chr4 exon 69322062 69322180 69322062 ID=MsG0480022178.01.T01:exon:55533;Parent=MsG0480022178.01.T01
Chr4 exon 69322370 69322781 69322370 ID=MsG0480022178.01.T01:exon:55532;Parent=MsG0480022178.01.T01
Chr4 five_prime_UTR 69322763 69322781 69322763 ID=MsG0480022178.01.T01:five_prime_utr;Parent=MsG0480022178.01.T01
Chr4 CDS 69322370 69322762 69322370 ID=MsG0480022178.01.T01:cds;Parent=MsG0480022178.01.T01
Chr4 CDS 69322062 69322180 69322062 ID=MsG0480022178.01.T01:cds;Parent=MsG0480022178.01.T01
Chr4 CDS 69321218 69321455 69321218 ID=MsG0480022178.01.T01:cds;Parent=MsG0480022178.01.T01
Gene Sequence

>MsG0480022178.01.T01

ATGGGTGCTTCTCTATCAGAGCTAGAGCAACCACCACAATCACAGCCACAACAAACACATCAACATTCACAGTTACAGCCACAACAACACCAACACCAACAAAATGAACCAACCAATAAGGAAGAATTACGATCACCTTCGAGAAGGGAAAATCTGTTGTCGTCTCCTCAACATCAAACAAAATTACCCTCCAGCAAGAAGGCAGTACAAAACTCTACAGTTGTAAAATCCAATGAGACTCAATCAGCAACCAAACCTTTGATCAAAGAACTAAGTTTGCCACATAACTATGAGTACATATTGAAGGATGCAGATTCACCGGTTGATAAATCTTCAAGGGAAAAGATGTGTGATCAGCTATATGCTGGAGTGTTCTTACATCATAAGACTAAGAAATATTGGGTAGAGAAGAACTCCAAAGCCAACTGTTTCATGTTGTATGCAAAAGCACTCTCAATCACCTGGGCAGAAGATCCAAATTATTGGAAATGGATACAGCAAAAAGATGTTAGCTCAGGTTGGGAACTTCCGGTGAATGTTCGACTTGTCCTTCCAGGAGGAAAGAAGCAGCAACACAAGGAAAATTTAATGGAAAAGTTAAGAGCACGGTGGATAGAGGTTCCAGTTGGTGAGTTTGTAGCATCTGAAAAAGATGGCGGTGAGATGGAGATATCAATGTTTGAATATGAAGGTGGTATGTGGAAACAAGGGCTTGTCATCAAAGGCATTGCTATCAAACCAAAGGAATGA

Protein sequence

>MsG0480022178.01.T01

MGASLSELEQPPQSQPQQTHQHSQLQPQQHQHQQNEPTNKEELRSPSRRENLLSSPQHQTKLPSSKKAVQNSTVVKSNETQSATKPLIKELSLPHNYEYILKDADSPVDKSSREKMCDQLYAGVFLHHKTKKYWVEKNSKANCFMLYAKALSITWAEDPNYWKWIQQKDVSSGWELPVNVRLVLPGGKKQQHKENLMEKLRARWIEVPVGEFVASEKDGGEMEISMFEYEGGMWKQGLVIKGIAIKPKE*