AlfalfaGEDB | MsG0480020449.01

M. sativa cultivar ZhongmuNo.1 / MsG0480020449.01

KEGG: -
GO: -
PFAM: PF03168

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480020449.01.T01	RHN60424.1	93.427	213	13	1	1	213	1	212	3.14E-142	405

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480020449.01.T01	A0A396I855	93.427	213	13	1	1	213	1	212	1.50e-142	405

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180000197.01	MsG0480020449.01	0.806861	6.695457e-50	3.673926e-47
MsG0180000821.01	MsG0480020449.01	0.804566	2.030985e-49	1.050642e-46
MsG0180004388.01	MsG0480020449.01	0.807243	5.560427e-50	3.081447e-47
MsG0280006356.01	MsG0480020449.01	0.804654	1.947221e-49	1.009595e-46
MsG0280010614.01	MsG0480020449.01	0.814491	1.499309e-51	1.007526e-48
MsG0280011052.01	MsG0480020449.01	0.805242	1.467514e-49	7.724724e-47
MsG0380011754.01	MsG0480020449.01	0.808335	3.257801e-50	1.857789e-47
MsG0380014135.01	MsG0480020449.01	0.807438	5.055298e-50	2.815681e-47
MsG0380014573.01	MsG0480020449.01	0.825096	5.645850e-54	5.096553e-51
MsG0380014913.01	MsG0480020449.01	0.802239	6.165840e-49	3.005030e-46
MsG0480018787.01	MsG0480020449.01	0.802462	5.547224e-49	2.718962e-46
MsG0480020242.01	MsG0480020449.01	0.833920	4.041098e-56	4.722258e-53
MsG0480020449.01	MsG0480021021.01	0.804892	1.736992e-49	9.062530e-47
MsG0480020449.01	MsG0480021695.01	0.803742	3.015004e-49	1.527003e-46
MsG0480020449.01	MsG0480022480.01	0.805417	1.348263e-49	7.129211e-47
MsG0480020449.01	MsG0480022597.01	0.822129	2.794243e-53	2.318238e-50
MsG0480020449.01	MsG0580024733.01	0.802098	6.590117e-49	3.200326e-46
MsG0480020449.01	MsG0580025008.01	0.815470	9.093397e-52	6.275471e-49
MsG0480020449.01	MsG0580025555.01	0.812441	4.234108e-51	2.692533e-48
MsG0480020449.01	MsG0580027401.01	0.801815	7.535269e-49	3.633098e-46
MsG0480020449.01	MsG0580027833.01	0.802682	4.995816e-49	2.462461e-46
MsG0480020449.01	MsG0580029189.01	0.806728	7.145084e-50	3.907050e-47
MsG0480020449.01	MsG0580029737.01	0.803352	3.632654e-49	1.821348e-46
MsG0480020449.01	MsG0680030685.01	0.817116	3.896701e-52	2.812777e-49
MsG0480020449.01	MsG0680034753.01	0.800517	1.388929e-48	6.480135e-46
MsG0480020449.01	MsG0680035656.01	0.813681	2.263300e-51	1.487804e-48
MsG0480020449.01	MsG0780036630.01	0.808577	2.892980e-50	1.660120e-47
MsG0480020449.01	MsG0780037859.01	0.805750	1.148266e-49	6.124225e-47
MsG0480020449.01	MsG0780038386.01	0.808917	2.446896e-50	1.416718e-47
MsG0480020449.01	MsG0780040679.01	0.810993	8.743138e-51	5.349574e-48
MsG0480020449.01	MsG0780040726.01	0.819168	1.338257e-52	1.022088e-49
MsG0480020449.01	MsG0780041220.01	0.801030	1.091185e-48	5.158129e-46
MsG0480020449.01	MsG0880045914.01	0.801337	9.443087e-49	4.498198e-46
MsG0480020449.01	MsG0880046244.01	0.800125	1.669277e-48	7.711069e-46
MsG0480020449.01	MsG0880046731.01	0.807314	5.369601e-50	2.981257e-47
MsG0380015878.01	MsG0480020449.01	0.816505	5.343366e-52	3.792956e-49
MsG0280008270.01	MsG0480020449.01	0.802625	5.132882e-49	2.526342e-46
MsG0280008295.01	MsG0480020449.01	0.803130	4.036900e-49	2.012591e-46
MsG0280010378.01	MsG0480020449.01	0.802970	4.357813e-49	2.163711e-46

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480020449.01.T01	MTR_4g051652	93.365	211	13	1	3	213	1	210	1.96e-144	401
MsG0480020449.01.T01	MTR_2g087080	56.122	196	85	1	13	207	16	211	1.95e-70	214
MsG0480020449.01.T01	MTR_4g051672	55.869	213	22	5	3	213	1	143	1.14e-64	197
MsG0480020449.01.T01	MTR_4g051705	89.888	89	9	0	125	213	1	89	5.10e-54	168
MsG0480020449.01.T01	MTR_4g051745	82.022	89	4	1	125	213	1	77	4.18e-45	145
MsG0480020449.01.T01	MTR_2g087000	27.179	195	139	2	14	208	39	230	2.31e-23	94.0
MsG0480020449.01.T01	MTR_2g011350	27.922	154	107	3	42	194	41	191	1.14e-13	67.4

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480020449.01.T01	AT3G52460	29.412	204	133	4	5	200	89	289	6.52e-19	83.2
MsG0480020449.01.T01	AT2G27260	27.950	161	111	4	47	206	84	240	5.88e-12	63.5

Find 50 sgRNAs with CRISPR-Local

Find 56 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTCGAAGTGGAGATACTTTA+CGG	0.315688	4:+41710492	MsG0480020449.01.T01:CDS
AAAAGACACTGGAAAACTAT+TGG	0.316716	4:+41710788	MsG0480020449.01.T01:CDS
TTCAATAACTAACTATGGTT+TGG	0.319426	4:-41710841	None:intergenic
TTGGATTTGAAGCTGGAATA+TGG	0.322794	4:+41710703	MsG0480020449.01.T01:CDS
CTGGAAAACTATTGGGTATT+GGG	0.345902	4:+41710796	MsG0480020449.01.T01:CDS
CGAAATTATCGAGAATCAAT+TGG	0.360477	4:-41710473	None:intergenic
GTATCTGATTCACAGATTAC+AGG	0.402907	4:+41710405	MsG0480020449.01.T01:CDS
CACCTCAGCTTCTGAGAGCA+TGG	0.413873	4:-41710520	None:intergenic
ATGTGTTTCTTGTGGGGACT+TGG	0.423348	4:+41710746	MsG0480020449.01.T01:CDS
ACTGGAAAACTATTGGGTAT+TGG	0.428456	4:+41710795	MsG0480020449.01.T01:CDS
TGGCTATCATTGGCATAGCT+TGG	0.438694	4:+41710319	MsG0480020449.01.T01:CDS
AAAGACACTGGAAAACTATT+GGG	0.440360	4:+41710789	MsG0480020449.01.T01:CDS
GGTGCAACAACCTATACTTT+TGG	0.440362	4:+41710539	MsG0480020449.01.T01:CDS
CTTGTTGGGATATTTCTAGT+AGG	0.444464	4:-41710228	None:intergenic
TTCTTGTGGGGACTTGGATG+TGG	0.458190	4:+41710752	MsG0480020449.01.T01:CDS
GTTAGAGTTGGATTTGAAGC+TGG	0.458770	4:+41710696	MsG0480020449.01.T01:CDS
ATGTCAGAAGATCTTCTTGT+TGG	0.462795	4:-41710243	None:intergenic
CTCATCTTCACCAAAAGTAT+AGG	0.463962	4:-41710549	None:intergenic
GTGAAGATGGTTGTTAGAGT+TGG	0.466909	4:+41710684	MsG0480020449.01.T01:CDS
TGTCAGAAGATCTTCTTGTT+GGG	0.479430	4:-41710242	None:intergenic
GTTGGGATATTTCTAGTAGG+AGG	0.485932	4:-41710225	None:intergenic
CAAGAGTATGAAGGTTGACT+TGG	0.487527	4:+41710575	MsG0480020449.01.T01:CDS
AGCCATGAAAATGACAAAGA+TGG	0.488088	4:-41710301	None:intergenic
AAGACATTATGTGTTTCTTG+TGG	0.490786	4:+41710738	MsG0480020449.01.T01:CDS
TTGGGATATTTCTAGTAGGA+GGG	0.498040	4:-41710224	None:intergenic
AGACATTATGTGTTTCTTGT+GGG	0.505280	4:+41710739	MsG0480020449.01.T01:CDS
ACAGATTACAGGTGTGTTCG+AGG	0.513498	4:+41710416	MsG0480020449.01.T01:CDS
TAGTTGCGGAGTGGAACAAA+GGG	0.526316	4:+41710646	MsG0480020449.01.T01:CDS
ATTACAGGTGTGTTCGAGGC+GGG	0.558603	4:+41710420	MsG0480020449.01.T01:CDS
GACATTATGTGTTTCTTGTG+GGG	0.561691	4:+41710740	MsG0480020449.01.T01:CDS
TTCAACGAAGTTCTTGCAGA+AGG	0.562601	4:+41710608	MsG0480020449.01.T01:CDS
TTCTATTCTACAAAAGACAC+TGG	0.567750	4:+41710777	MsG0480020449.01.T01:CDS
CTAGTTGCGGAGTGGAACAA+AGG	0.586760	4:+41710645	MsG0480020449.01.T01:CDS
GATGAGCAACAAGAGTATGA+AGG	0.586934	4:+41710566	MsG0480020449.01.T01:CDS
AGCTGGAATATGGCCAAGCA+AGG	0.592271	4:+41710713	MsG0480020449.01.T01:CDS
AACCATGCTCTCAGAAGCTG+AGG	0.598904	4:+41710518	MsG0480020449.01.T01:CDS
AACGAAGTTCTTGCAGAAGG+CGG	0.601484	4:+41710611	MsG0480020449.01.T01:CDS
ACCGCAAAACGAGGGTCGTG+TGG	0.611743	4:-41710354	None:intergenic
ACAAGAAGATCTTCTGACAT+CGG	0.621164	4:+41710246	MsG0480020449.01.T01:CDS
AAAATCTAAAGTAATGAACA+TGG	0.629473	4:+41710197	None:intergenic
GATTCTCGATAATTTCGAAG+TGG	0.629496	4:+41710479	MsG0480020449.01.T01:CDS
AGTGATGTTACCGCAAAACG+AGG	0.645009	4:-41710363	None:intergenic
GTGATGTTACCGCAAAACGA+GGG	0.671564	4:-41710362	None:intergenic
TGCGGAGTGGAACAAAGGGG+TGG	0.672319	4:+41710650	MsG0480020449.01.T01:CDS
GGTGCAAGAGAATCTAGTTG+CGG	0.677413	4:+41710632	MsG0480020449.01.T01:CDS
CCGGCGTCTGACGTTGACAG+TGG	0.680314	4:+41710275	MsG0480020449.01.T01:CDS
CCACTGTCAACGTCAGACGC+CGG	0.687949	4:-41710275	None:intergenic
GATTACAGGTGTGTTCGAGG+CGG	0.690033	4:+41710419	MsG0480020449.01.T01:CDS
AAGAGAATCTAGTTGCGGAG+TGG	0.723007	4:+41710637	MsG0480020449.01.T01:CDS
AGTTGCGGAGTGGAACAAAG+GGG	0.746823	4:+41710647	MsG0480020449.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TCAATTGGATTTTTTTGTTT+TGG	-	Chr4:41710461-41710480	None:intergenic	20.0%
!!!	TTTTTTGTTTTGGTTTGTTA+TGG	-	Chr4:41710451-41710470	None:intergenic	20.0%
	AAAAGACACTGGAAAACTAT+TGG	+	Chr4:41710788-41710807	MsG0480020449.01.T01:CDS	30.0%
	AAAGACACTGGAAAACTATT+GGG	+	Chr4:41710789-41710808	MsG0480020449.01.T01:CDS	30.0%
	AAGACATTATGTGTTTCTTG+TGG	+	Chr4:41710738-41710757	MsG0480020449.01.T01:CDS	30.0%
	AGACATTATGTGTTTCTTGT+GGG	+	Chr4:41710739-41710758	MsG0480020449.01.T01:CDS	30.0%
	CGAAATTATCGAGAATCAAT+TGG	-	Chr4:41710476-41710495	None:intergenic	30.0%
	TTCTATTCTACAAAAGACAC+TGG	+	Chr4:41710777-41710796	MsG0480020449.01.T01:CDS	30.0%
!!!	ATCCATCTTTGTCATTTTCA+TGG	+	Chr4:41710299-41710318	MsG0480020449.01.T01:CDS	30.0%
	ACAAGAAGATCTTCTGACAT+CGG	+	Chr4:41710246-41710265	MsG0480020449.01.T01:CDS	35.0%
	ACTGGAAAACTATTGGGTAT+TGG	+	Chr4:41710795-41710814	MsG0480020449.01.T01:CDS	35.0%
	AGCCATGAAAATGACAAAGA+TGG	-	Chr4:41710304-41710323	None:intergenic	35.0%
	ATGTCAGAAGATCTTCTTGT+TGG	-	Chr4:41710246-41710265	None:intergenic	35.0%
	CTGGAAAACTATTGGGTATT+GGG	+	Chr4:41710796-41710815	MsG0480020449.01.T01:CDS	35.0%
	GACATTATGTGTTTCTTGTG+GGG	+	Chr4:41710740-41710759	MsG0480020449.01.T01:CDS	35.0%
	GATTCTCGATAATTTCGAAG+TGG	+	Chr4:41710479-41710498	MsG0480020449.01.T01:CDS	35.0%
	GTATCTGATTCACAGATTAC+AGG	+	Chr4:41710405-41710424	MsG0480020449.01.T01:CDS	35.0%
	TGTCAGAAGATCTTCTTGTT+GGG	-	Chr4:41710245-41710264	None:intergenic	35.0%
	TTCGAAGTGGAGATACTTTA+CGG	+	Chr4:41710492-41710511	MsG0480020449.01.T01:CDS	35.0%
!	CATAATGTCTTTTCCTTGCT+TGG	-	Chr4:41710729-41710748	None:intergenic	35.0%
!	CTCATCTTCACCAAAAGTAT+AGG	-	Chr4:41710552-41710571	None:intergenic	35.0%
!	TTGGATTTGAAGCTGGAATA+TGG	+	Chr4:41710703-41710722	MsG0480020449.01.T01:CDS	35.0%
!!	CTTGTTGGGATATTTCTAGT+AGG	-	Chr4:41710231-41710250	None:intergenic	35.0%
!!	GGTGAATTTTGATGTGAAGA+TGG	+	Chr4:41710671-41710690	MsG0480020449.01.T01:CDS	35.0%
!!	GTCATTTTCATGGCTATCAT+TGG	+	Chr4:41710309-41710328	MsG0480020449.01.T01:CDS	35.0%
!!	TTGGGATATTTCTAGTAGGA+GGG	-	Chr4:41710227-41710246	None:intergenic	35.0%
	GTGAAGATGGTTGTTAGAGT+TGG	+	Chr4:41710684-41710703	MsG0480020449.01.T01:CDS	40.0%
	TTCAACGAAGTTCTTGCAGA+AGG	+	Chr4:41710608-41710627	MsG0480020449.01.T01:CDS	40.0%
!	CAAGAGTATGAAGGTTGACT+TGG	+	Chr4:41710575-41710594	MsG0480020449.01.T01:CDS	40.0%
!	GATGAGCAACAAGAGTATGA+AGG	+	Chr4:41710566-41710585	MsG0480020449.01.T01:CDS	40.0%
!	GGTGCAACAACCTATACTTT+TGG	+	Chr4:41710539-41710558	MsG0480020449.01.T01:CDS	40.0%
!	GTTAGAGTTGGATTTGAAGC+TGG	+	Chr4:41710696-41710715	MsG0480020449.01.T01:CDS	40.0%
!!	GTTGGGATATTTCTAGTAGG+AGG	-	Chr4:41710228-41710247	None:intergenic	40.0%
	AACGAAGTTCTTGCAGAAGG+CGG	+	Chr4:41710611-41710630	MsG0480020449.01.T01:CDS	45.0%
	AAGAGAATCTAGTTGCGGAG+TGG	+	Chr4:41710637-41710656	MsG0480020449.01.T01:CDS	45.0%
	ACAGATTACAGGTGTGTTCG+AGG	+	Chr4:41710416-41710435	MsG0480020449.01.T01:CDS	45.0%
	AGTGATGTTACCGCAAAACG+AGG	-	Chr4:41710366-41710385	None:intergenic	45.0%
	ATGTGTTTCTTGTGGGGACT+TGG	+	Chr4:41710746-41710765	MsG0480020449.01.T01:CDS	45.0%
	GGTGCAAGAGAATCTAGTTG+CGG	+	Chr4:41710632-41710651	MsG0480020449.01.T01:CDS	45.0%
	GTGATGTTACCGCAAAACGA+GGG	-	Chr4:41710365-41710384	None:intergenic	45.0%
	TAGTTGCGGAGTGGAACAAA+GGG	+	Chr4:41710646-41710665	MsG0480020449.01.T01:CDS	45.0%
	TGGCTATCATTGGCATAGCT+TGG	+	Chr4:41710319-41710338	MsG0480020449.01.T01:CDS	45.0%
!!!	CTTCTGACATCGGTTTTGTC+CGG	+	Chr4:41710256-41710275	MsG0480020449.01.T01:CDS	45.0%
	AACCATGCTCTCAGAAGCTG+AGG	+	Chr4:41710518-41710537	MsG0480020449.01.T01:CDS	50.0%
	AGCTGGAATATGGCCAAGCA+AGG	+	Chr4:41710713-41710732	MsG0480020449.01.T01:CDS	50.0%
	AGTTGCGGAGTGGAACAAAG+GGG	+	Chr4:41710647-41710666	MsG0480020449.01.T01:CDS	50.0%
	ATTACAGGTGTGTTCGAGGC+GGG	+	Chr4:41710420-41710439	MsG0480020449.01.T01:CDS	50.0%
	CTAGTTGCGGAGTGGAACAA+AGG	+	Chr4:41710645-41710664	MsG0480020449.01.T01:CDS	50.0%
	GATTACAGGTGTGTTCGAGG+CGG	+	Chr4:41710419-41710438	MsG0480020449.01.T01:CDS	50.0%
	TTCTTGTGGGGACTTGGATG+TGG	+	Chr4:41710752-41710771	MsG0480020449.01.T01:CDS	50.0%
!	CACCTCAGCTTCTGAGAGCA+TGG	-	Chr4:41710523-41710542	None:intergenic	55.0%
!	TCCACACGACCCTCGTTTTG+CGG	+	Chr4:41710353-41710372	MsG0480020449.01.T01:CDS	55.0%
	ACCGCAAAACGAGGGTCGTG+TGG	-	Chr4:41710357-41710376	None:intergenic	60.0%
	CCACTGTCAACGTCAGACGC+CGG	-	Chr4:41710278-41710297	None:intergenic	60.0%
	TGCGGAGTGGAACAAAGGGG+TGG	+	Chr4:41710650-41710669	MsG0480020449.01.T01:CDS	60.0%
	CCGGCGTCTGACGTTGACAG+TGG	+	Chr4:41710275-41710294	MsG0480020449.01.T01:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
Chr4	gene	41710210	41710851	41710210	ID=MsG0480020449.01;Name=MsG0480020449.01
Chr4	mRNA	41710210	41710851	41710210	ID=MsG0480020449.01.T01;Parent=MsG0480020449.01;Name=MsG0480020449.01.T01;_AED=0.46;_eAED=0.46;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|213
Chr4	exon	41710210	41710851	41710210	ID=MsG0480020449.01.T01:exon:15326;Parent=MsG0480020449.01.T01
Chr4	CDS	41710210	41710851	41710210	ID=MsG0480020449.01.T01:cds;Parent=MsG0480020449.01.T01

Gene Sequence

>MsG0480020449.01.T01

ATGAACATGGAGCACCCTCCTACTAGAAATATCCCAACAAGAAGATCTTCTGACATCGGTTTTGTCCGGCGTCTGACGTTGACAGTGGTATCCATCTTTGTCATTTTCATGGCTATCATTGGCATAGCTTGGTTTGTCATGCATCCACACGACCCTCGTTTTGCGGTAACATCACTTTCTGTCTCAAACTTCACTGTATCTGATTCACAGATTACAGGTGTGTTCGAGGCGGGACTAACCATAACAAACCAAAACAAAAAAATCCAATTGATTCTCGATAATTTCGAAGTGGAGATACTTTACGGTGAAACCATGCTCTCAGAAGCTGAGGTGCAACAACCTATACTTTTGGTGAAGATGAGCAACAAGAGTATGAAGGTTGACTTGGTAACGACGAATTCAACGAAGTTCTTGCAGAAGGCGGTGCAAGAGAATCTAGTTGCGGAGTGGAACAAAGGGGTGGTGAATTTTGATGTGAAGATGGTTGTTAGAGTTGGATTTGAAGCTGGAATATGGCCAAGCAAGGAAAAGACATTATGTGTTTCTTGTGGGGACTTGGATGTGGAATTCTATTCTACAAAAGACACTGGAAAACTATTGGGTATTGGGAAGAACTGCAACATAGTTGAAGCCAAACCATAG

Protein sequence

>MsG0480020449.01.T01

MNMEHPPTRNIPTRRSSDIGFVRRLTLTVVSIFVIFMAIIGIAWFVMHPHDPRFAVTSLSVSNFTVSDSQITGVFEAGLTITNQNKKIQLILDNFEVEILYGETMLSEAEVQQPILLVKMSNKSMKVDLVTTNSTKFLQKAVQENLVAEWNKGVVNFDVKMVVRVGFEAGIWPSKEKTLCVSCGDLDVEFYSTKDTGKLLGIGKNCNIVEAKP*