Alfalfa Gene Editing Database
Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsG0780037859.01.T01 | NP_068729.1 | 71.569 | 102 | 29 | 0 | 1 | 102 | 587 | 688 | 1.33E-42 | 158 |
Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsG0780037859.01.T01 | P15629 | 71.569 | 102 | 29 | 0 | 1 | 102 | 587 | 688 | 1.65E-45 | 158 |
Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsG0780037859.01.T01 | A0A4D6U452 | 71.569 | 102 | 29 | 0 | 1 | 102 | 587 | 688 | 6.35e-43 | 158 |
Gene ID | Type | Classification |
---|
Gene ID | Type | Classification |
---|
Co-expression Network
Gene1 | Gene2 | correlation coefficient | p_value | FDR |
---|---|---|---|---|
MsG0080047797.01 | MsG0780037859.01 | 0.826820 | 2.198737e-54 | 2.084713e-51 |
MsG0180000091.01 | MsG0780037859.01 | 0.809012 | 2.334862e-50 | 1.355301e-47 |
MsG0180000154.01 | MsG0780037859.01 | 0.816979 | 4.182009e-52 | 3.007749e-49 |
MsG0180000470.01 | MsG0780037859.01 | 0.854220 | 1.423396e-61 | 3.156605e-58 |
MsG0180000526.01 | MsG0780037859.01 | 0.813658 | 2.290018e-51 | 1.504422e-48 |
MsG0180000555.01 | MsG0780037859.01 | 0.800503 | 1.397809e-48 | 6.519208e-46 |
MsG0180000702.01 | MsG0780037859.01 | 0.807369 | 5.228039e-50 | 2.906667e-47 |
MsG0180000821.01 | MsG0780037859.01 | 0.809277 | 2.049276e-50 | 1.198040e-47 |
MsG0180000822.01 | MsG0780037859.01 | 0.806661 | 7.379832e-50 | 4.028299e-47 |
MsG0180001971.01 | MsG0780037859.01 | 0.810172 | 1.315645e-50 | 7.875131e-48 |
MsG0180002894.01 | MsG0780037859.01 | 0.806746 | 7.080633e-50 | 3.873652e-47 |
MsG0180004264.01 | MsG0780037859.01 | 0.815088 | 1.105921e-51 | 7.551690e-49 |
MsG0180004388.01 | MsG0780037859.01 | 0.803118 | 4.061356e-49 | 2.024126e-46 |
MsG0180004465.01 | MsG0780037859.01 | 0.801761 | 7.731346e-49 | 3.722298e-46 |
MsG0180004765.01 | MsG0780037859.01 | 0.808062 | 3.723778e-50 | 2.108190e-47 |
MsG0180005185.01 | MsG0780037859.01 | 0.801397 | 9.181884e-49 | 4.380225e-46 |
MsG0180005507.01 | MsG0780037859.01 | 0.816183 | 6.306120e-52 | 4.437422e-49 |
MsG0180005944.01 | MsG0780037859.01 | 0.800049 | 1.729546e-48 | 7.974035e-46 |
MsG0280006375.01 | MsG0780037859.01 | 0.804359 | 2.243311e-49 | 1.154327e-46 |
MsG0280010614.01 | MsG0780037859.01 | 0.801893 | 7.263768e-49 | 3.509246e-46 |
MsG0280011163.01 | MsG0780037859.01 | 0.830413 | 2.979056e-55 | 3.136325e-52 |
MsG0380011494.01 | MsG0780037859.01 | 0.806595 | 7.621791e-50 | 4.153371e-47 |
MsG0380011663.01 | MsG0780037859.01 | 0.815428 | 9.291449e-52 | 6.404348e-49 |
MsG0380014288.01 | MsG0780037859.01 | 0.800832 | 1.197564e-48 | 5.632279e-46 |
MsG0380014573.01 | MsG0780037859.01 | 0.836593 | 8.544743e-57 | 1.081782e-53 |
MsG0380014894.01 | MsG0780037859.01 | 0.836151 | 1.107202e-56 | 1.383354e-53 |
MsG0380014902.01 | MsG0780037859.01 | 0.808836 | 2.546876e-50 | 1.471403e-47 |
MsG0680032460.01 | MsG0780037859.01 | -0.801229 | 9.936884e-49 | 4.720537e-46 |
MsG0680032801.01 | MsG0780037859.01 | 0.812211 | 4.751898e-51 | 3.003000e-48 |
MsG0680032996.01 | MsG0780037859.01 | 0.814430 | 1.546786e-51 | 1.037684e-48 |
MsG0680034753.01 | MsG0780037859.01 | 0.806242 | 9.047430e-50 | 4.886380e-47 |
MsG0680035123.01 | MsG0780037859.01 | 0.817852 | 2.659832e-52 | 1.959177e-49 |
MsG0680035444.01 | MsG0780037859.01 | 0.830314 | 3.149596e-55 | 3.306314e-52 |
MsG0680035445.01 | MsG0780037859.01 | 0.802699 | 4.957001e-49 | 2.444423e-46 |
MsG0680035455.01 | MsG0780037859.01 | 0.818208 | 2.209770e-52 | 1.643605e-49 |
MsG0480018640.01 | MsG0780037859.01 | 0.805049 | 1.610041e-49 | 8.433337e-47 |
MsG0480019435.01 | MsG0780037859.01 | 0.826028 | 3.396735e-54 | 3.148567e-51 |
MsG0480019970.01 | MsG0780037859.01 | 0.810388 | 1.181753e-50 | 7.113687e-48 |
MsG0480020167.01 | MsG0780037859.01 | 0.802632 | 5.115578e-49 | 2.518310e-46 |
MsG0480020202.01 | MsG0780037859.01 | 0.803434 | 3.492814e-49 | 1.755014e-46 |
MsG0480020449.01 | MsG0780037859.01 | 0.805750 | 1.148266e-49 | 6.124225e-47 |
MsG0480020964.01 | MsG0780037859.01 | 0.805939 | 1.047589e-49 | 5.614331e-47 |
MsG0480021536.01 | MsG0780037859.01 | 0.806852 | 6.727304e-50 | 3.690457e-47 |
MsG0480021784.01 | MsG0780037859.01 | 0.800940 | 1.138414e-48 | 5.368896e-46 |
MsG0480021927.01 | MsG0780037859.01 | 0.816906 | 4.342515e-52 | 3.116721e-49 |
MsG0480022533.01 | MsG0780037859.01 | 0.819209 | 1.309966e-52 | 1.001640e-49 |
MsG0480022575.01 | MsG0780037859.01 | 0.830767 | 2.439280e-55 | 2.595055e-52 |
MsG0480023248.01 | MsG0780037859.01 | 0.829594 | 4.717991e-55 | 4.850923e-52 |
MsG0480023654.01 | MsG0780037859.01 | 0.810687 | 1.018464e-50 | 6.180178e-48 |
MsG0480023670.01 | MsG0780037859.01 | 0.804054 | 2.597231e-49 | 1.326034e-46 |
MsG0580024114.01 | MsG0780037859.01 | 0.816548 | 5.225997e-52 | 3.714013e-49 |
MsG0580024305.01 | MsG0780037859.01 | 0.805778 | 1.132881e-49 | 6.046322e-47 |
MsG0580024363.01 | MsG0780037859.01 | -0.833037 | 6.711502e-56 | 7.637344e-53 |
MsG0580024433.01 | MsG0780037859.01 | 0.801621 | 8.257463e-49 | 3.961594e-46 |
MsG0580024690.01 | MsG0780037859.01 | 0.840161 | 1.027071e-57 | 1.450626e-54 |
MsG0580024853.01 | MsG0780037859.01 | 0.803078 | 4.138253e-49 | 2.060336e-46 |
MsG0580025008.01 | MsG0780037859.01 | 0.806279 | 8.887485e-50 | 4.804352e-47 |
MsG0580025532.01 | MsG0780037859.01 | 0.803114 | 4.068528e-49 | 2.027497e-46 |
MsG0580025555.01 | MsG0780037859.01 | 0.805762 | 1.141299e-49 | 6.088955e-47 |
MsG0380015877.01 | MsG0780037859.01 | 0.827593 | 1.435809e-54 | 1.392256e-51 |
MsG0380015878.01 | MsG0780037859.01 | 0.834294 | 3.256444e-56 | 3.848120e-53 |
MsG0380016859.01 | MsG0780037859.01 | 0.808555 | 2.924443e-50 | 1.677219e-47 |
MsG0380016910.01 | MsG0780037859.01 | 0.821265 | 4.425114e-53 | 3.582610e-50 |
MsG0380017220.01 | MsG0780037859.01 | 0.806814 | 6.852475e-50 | 3.755453e-47 |
MsG0380017233.01 | MsG0780037859.01 | 0.800701 | 1.273602e-48 | 5.969812e-46 |
MsG0480018251.01 | MsG0780037859.01 | 0.800994 | 1.109931e-48 | 5.242006e-46 |
MsG0480018321.01 | MsG0780037859.01 | 0.805175 | 1.515284e-49 | 7.962801e-47 |
MsG0580027152.01 | MsG0780037859.01 | 0.802389 | 5.743057e-49 | 2.809841e-46 |
MsG0580027401.01 | MsG0780037859.01 | 0.806845 | 6.748534e-50 | 3.701506e-47 |
MsG0580027833.01 | MsG0780037859.01 | 0.831255 | 1.851471e-55 | 1.998103e-52 |
MsG0580028527.01 | MsG0780037859.01 | 0.807428 | 5.080135e-50 | 2.828820e-47 |
MsG0580029008.01 | MsG0780037859.01 | 0.814073 | 1.854702e-51 | 1.232268e-48 |
MsG0580029554.01 | MsG0780037859.01 | 0.801832 | 7.475374e-49 | 3.605755e-46 |
MsG0580029737.01 | MsG0780037859.01 | 0.804464 | 2.133057e-49 | 1.100539e-46 |
MsG0680030629.01 | MsG0780037859.01 | 0.841671 | 4.125444e-58 | 6.105536e-55 |
MsG0680030694.01 | MsG0780037859.01 | 0.815800 | 7.677564e-52 | 5.346341e-49 |
MsG0280006770.01 | MsG0780037859.01 | 0.812059 | 5.129663e-51 | 3.228111e-48 |
MsG0280007529.01 | MsG0780037859.01 | 0.808752 | 2.654568e-50 | 1.530285e-47 |
MsG0280007547.01 | MsG0780037859.01 | 0.833792 | 4.350035e-56 | 5.063365e-53 |
MsG0280007826.01 | MsG0780037859.01 | 0.831829 | 1.337274e-55 | 1.468265e-52 |
MsG0280008073.01 | MsG0780037859.01 | 0.822124 | 2.800853e-53 | 2.323401e-50 |
MsG0280008270.01 | MsG0780037859.01 | 0.805225 | 1.479327e-49 | 7.783602e-47 |
MsG0280008615.01 | MsG0780037859.01 | 0.804722 | 1.884722e-49 | 9.789835e-47 |
MsG0280010378.01 | MsG0780037859.01 | 0.816710 | 4.805638e-52 | 3.430560e-49 |
MsG0280010443.01 | MsG0780037859.01 | 0.831290 | 1.814854e-55 | 1.960697e-52 |
MsG0680035844.01 | MsG0780037859.01 | 0.840473 | 8.511986e-58 | 1.213749e-54 |
MsG0780035948.01 | MsG0780037859.01 | 0.803685 | 3.098319e-49 | 1.566866e-46 |
MsG0780036630.01 | MsG0780037859.01 | 0.801395 | 9.186623e-49 | 4.382385e-46 |
MsG0780037859.01 | MsG0780038401.01 | 0.827221 | 1.763144e-54 | 1.691427e-51 |
MsG0780037859.01 | MsG0780038825.01 | 0.808154 | 3.560599e-50 | 2.020777e-47 |
MsG0780037859.01 | MsG0780039252.01 | 0.814936 | 1.195089e-51 | 8.127088e-49 |
MsG0780037859.01 | MsG0780040576.01 | -0.803690 | 3.090561e-49 | 1.563182e-46 |
MsG0780037859.01 | MsG0780040704.01 | 0.803346 | 3.642188e-49 | 1.825916e-46 |
MsG0780037859.01 | MsG0780040726.01 | 0.824489 | 7.852872e-54 | 6.965921e-51 |
MsG0780037859.01 | MsG0780041456.01 | 0.802630 | 5.121797e-49 | 2.521184e-46 |
MsG0780037859.01 | MsG0780041485.01 | 0.800151 | 1.648644e-48 | 7.620729e-46 |
MsG0780037859.01 | MsG0880042053.01 | 0.801906 | 7.217656e-49 | 3.488030e-46 |
MsG0780037859.01 | MsG0880042611.01 | 0.804903 | 1.727762e-49 | 9.016811e-47 |
MsG0780037859.01 | MsG0880042656.01 | 0.815680 | 8.166011e-52 | 5.667761e-49 |
MsG0780037859.01 | MsG0880044079.01 | 0.805038 | 1.619025e-49 | 8.477836e-47 |
MsG0780037859.01 | MsG0880044174.01 | 0.807451 | 5.022010e-50 | 2.798221e-47 |
MsG0780037859.01 | MsG0880044573.01 | 0.808687 | 2.740645e-50 | 1.577172e-47 |
MsG0780037859.01 | MsG0880044574.01 | 0.811596 | 6.467186e-51 | 4.019777e-48 |
MsG0780037859.01 | MsG0880045314.01 | 0.811626 | 6.371556e-51 | 3.963479e-48 |
MsG0780037859.01 | MsG0880045590.01 | 0.814054 | 1.873098e-51 | 1.243782e-48 |
MsG0780037859.01 | MsG0880045639.01 | 0.807065 | 6.063734e-50 | 3.344744e-47 |
MsG0780037859.01 | MsG0880045895.01 | 0.812223 | 4.723884e-51 | 2.986337e-48 |
MsG0780037859.01 | MsG0880046020.01 | 0.802181 | 6.337649e-49 | 3.084125e-46 |
MsG0780037859.01 | MsG0880046967.01 | 0.802045 | 6.757863e-49 | 3.277353e-46 |
PPI
Gene1 | Gene2 | Type |
---|
Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|
Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|
Find 13 sgRNAs with CRISPR-Local
Find 13 sgRNAs with CRISPR-GE
CRISPR-Local
sgRNA_sequence | on_target_score | Position | Region |
---|---|---|---|
CCAAGTATCTTACAGGTTTC+TGG | 0.108284 | 7:-34828230 | MsG0780037859.01.T01:CDS |
TTGCTCTGTAAATACATTTC+AGG | 0.198100 | 7:-34828375 | MsG0780037859.01.T01:CDS |
ACAGATTCCAAGTATCTTAC+AGG | 0.284922 | 7:-34828237 | MsG0780037859.01.T01:CDS |
GTGTTTCCAGTTCAGAAATA+TGG | 0.288560 | 7:+34828325 | None:intergenic |
CCAGAAACCTGTAAGATACT+TGG | 0.375003 | 7:+34828230 | None:intergenic |
CGAGAATGGAGCAAACAATA+AGG | 0.379884 | 7:-34828081 | None:intergenic |
TAATTAGTCTACCTTGTCTA+TGG | 0.409346 | 7:+34828187 | None:intergenic |
AAGATACCATATTTCTGAAC+TGG | 0.412021 | 7:-34828331 | MsG0780037859.01.T01:CDS |
GACAAGGTAGACTAATTAGA+TGG | 0.447879 | 7:-34828182 | MsG0780037859.01.T01:CDS |
TTATGTATTGAATGTATGGT+TGG | 0.512093 | 7:+34828139 | None:intergenic |
CAGATACACTCACTCGAGAA+TGG | 0.515785 | 7:-34828095 | MsG0780037859.01.T01:CDS |
TATTGAATGTATGGTTGGAA+TGG | 0.575495 | 7:+34828144 | None:intergenic |
ATTAAAGCTGACCATAGACA+AGG | 0.678257 | 7:-34828198 | MsG0780037859.01.T01:CDS |
CRISPR-GE
badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
---|---|---|---|---|---|
!!! | GATTTTATGTATTGAATGTA+TGG | + | Chr7:34828323-34828342 | None:intergenic | 20.0% |
! | GTTCGTAATAAAAATCTTGA+TGG | + | Chr7:34828200-34828219 | None:intergenic | 25.0% |
! | TTATGTATTGAATGTATGGT+TGG | + | Chr7:34828319-34828338 | None:intergenic | 25.0% |
TATTGAATGTATGGTTGGAA+TGG | + | Chr7:34828314-34828333 | None:intergenic | 30.0% | |
! | AAGATACCATATTTCTGAAC+TGG | - | Chr7:34828124-34828143 | MsG0780037859.01.T01:CDS | 30.0% |
! | TAATTAGTCTACCTTGTCTA+TGG | + | Chr7:34828271-34828290 | None:intergenic | 30.0% |
ACAGATTCCAAGTATCTTAC+AGG | - | Chr7:34828218-34828237 | MsG0780037859.01.T01:CDS | 35.0% | |
ATTAAAGCTGACCATAGACA+AGG | - | Chr7:34828257-34828276 | MsG0780037859.01.T01:CDS | 35.0% | |
GACAAGGTAGACTAATTAGA+TGG | - | Chr7:34828273-34828292 | MsG0780037859.01.T01:CDS | 35.0% | |
GTGTTTCCAGTTCAGAAATA+TGG | + | Chr7:34828133-34828152 | None:intergenic | 35.0% | |
CCAAGTATCTTACAGGTTTC+TGG | - | Chr7:34828225-34828244 | MsG0780037859.01.T01:CDS | 40.0% | |
CCAGAAACCTGTAAGATACT+TGG | + | Chr7:34828228-34828247 | None:intergenic | 40.0% | |
CAGATACACTCACTCGAGAA+TGG | - | Chr7:34828360-34828379 | MsG0780037859.01.T01:CDS | 45.0% |
Chromosome | Type | Strat | End | Strand | Name |
---|---|---|---|---|---|
Chr7 | gene | 34828083 | 34828394 | 34828083 | ID=MsG0780037859.01;Name=MsG0780037859.01 |
Chr7 | mRNA | 34828083 | 34828394 | 34828083 | ID=MsG0780037859.01.T01;Parent=MsG0780037859.01;Name=MsG0780037859.01.T01;_AED=0.47;_eAED=0.47;_QI=0|-1|0|1|-1|1|1|0|103 |
Chr7 | exon | 34828083 | 34828394 | 34828083 | ID=MsG0780037859.01.T01:exon:15951;Parent=MsG0780037859.01.T01 |
Chr7 | CDS | 34828083 | 34828394 | 34828083 | ID=MsG0780037859.01.T01:cds;Parent=MsG0780037859.01.T01 |
Gene Sequence |
Protein sequence |