AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780037859.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 13 sgRNAs with CRISPR-Local

Find 13 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCAAGTATCTTACAGGTTTC+TGG 0.108284 7:-34828230 MsG0780037859.01.T01:CDS
TTGCTCTGTAAATACATTTC+AGG 0.198100 7:-34828375 MsG0780037859.01.T01:CDS
ACAGATTCCAAGTATCTTAC+AGG 0.284922 7:-34828237 MsG0780037859.01.T01:CDS
GTGTTTCCAGTTCAGAAATA+TGG 0.288560 7:+34828325 None:intergenic
CCAGAAACCTGTAAGATACT+TGG 0.375003 7:+34828230 None:intergenic
CGAGAATGGAGCAAACAATA+AGG 0.379884 7:-34828081 None:intergenic
TAATTAGTCTACCTTGTCTA+TGG 0.409346 7:+34828187 None:intergenic
AAGATACCATATTTCTGAAC+TGG 0.412021 7:-34828331 MsG0780037859.01.T01:CDS
GACAAGGTAGACTAATTAGA+TGG 0.447879 7:-34828182 MsG0780037859.01.T01:CDS
TTATGTATTGAATGTATGGT+TGG 0.512093 7:+34828139 None:intergenic
CAGATACACTCACTCGAGAA+TGG 0.515785 7:-34828095 MsG0780037859.01.T01:CDS
TATTGAATGTATGGTTGGAA+TGG 0.575495 7:+34828144 None:intergenic
ATTAAAGCTGACCATAGACA+AGG 0.678257 7:-34828198 MsG0780037859.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GATTTTATGTATTGAATGTA+TGG + Chr7:34828323-34828342 None:intergenic 20.0%
! GTTCGTAATAAAAATCTTGA+TGG + Chr7:34828200-34828219 None:intergenic 25.0%
! TTATGTATTGAATGTATGGT+TGG + Chr7:34828319-34828338 None:intergenic 25.0%
TATTGAATGTATGGTTGGAA+TGG + Chr7:34828314-34828333 None:intergenic 30.0%
! AAGATACCATATTTCTGAAC+TGG - Chr7:34828124-34828143 MsG0780037859.01.T01:CDS 30.0%
! TAATTAGTCTACCTTGTCTA+TGG + Chr7:34828271-34828290 None:intergenic 30.0%
ACAGATTCCAAGTATCTTAC+AGG - Chr7:34828218-34828237 MsG0780037859.01.T01:CDS 35.0%
ATTAAAGCTGACCATAGACA+AGG - Chr7:34828257-34828276 MsG0780037859.01.T01:CDS 35.0%
GACAAGGTAGACTAATTAGA+TGG - Chr7:34828273-34828292 MsG0780037859.01.T01:CDS 35.0%
GTGTTTCCAGTTCAGAAATA+TGG + Chr7:34828133-34828152 None:intergenic 35.0%
CCAAGTATCTTACAGGTTTC+TGG - Chr7:34828225-34828244 MsG0780037859.01.T01:CDS 40.0%
CCAGAAACCTGTAAGATACT+TGG + Chr7:34828228-34828247 None:intergenic 40.0%
CAGATACACTCACTCGAGAA+TGG - Chr7:34828360-34828379 MsG0780037859.01.T01:CDS 45.0%
Chromosome Type Strat End Strand Name
Chr7 gene 34828083 34828394 34828083 ID=MsG0780037859.01;Name=MsG0780037859.01
Chr7 mRNA 34828083 34828394 34828083 ID=MsG0780037859.01.T01;Parent=MsG0780037859.01;Name=MsG0780037859.01.T01;_AED=0.47;_eAED=0.47;_QI=0|-1|0|1|-1|1|1|0|103
Chr7 exon 34828083 34828394 34828083 ID=MsG0780037859.01.T01:exon:15951;Parent=MsG0780037859.01.T01
Chr7 CDS 34828083 34828394 34828083 ID=MsG0780037859.01.T01:cds;Parent=MsG0780037859.01.T01
Gene Sequence

>MsG0780037859.01.T01

CTCTGTAAATACATTTCAGGATCTTTTACAGATACTGAGACAAGATACCATATTTCTGAACTGGAAACACTATCTTGTGTTAAAGTCTTAGAAAAACATCAAATTGAATTACTTCCATCAAGATTTTTATTACGAACAGATTCCAAGTATCTTACAGGTTTCTGGAAATACAAAATTAAAGCTGACCATAGACAAGGTAGACTAATTAGATGGCAAATGAAGTTACAACCATTCCAACCATACATTCAATACATAAAATCTGAACAAAACAGTTTTGCAGATACACTCACTCGAGAATGGAGCAAACAATAA

Protein sequence

>MsG0780037859.01.T01

LCKYISGSFTDTETRYHISELETLSCVKVLEKHQIELLPSRFLLRTDSKYLTGFWKYKIKADHRQGRLIRWQMKLQPFQPYIQYIKSEQNSFADTLTREWSKQ*