AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780040726.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780040726.01.T01 MTR_7g096720 77.714 175 7 1 5 147 208 382 3.05e-92 272
MsG0780040726.01.T01 MTR_4g088925 61.932 176 35 1 4 147 206 381 8.99e-68 210
MsG0780040726.01.T01 MTR_8g085760 48.824 170 54 3 9 146 215 383 1.81e-48 160
MsG0780040726.01.T01 MTR_6g092570 42.138 159 68 3 6 143 193 348 2.40e-27 104
MsG0780040726.01.T01 MTR_8g074610 39.216 153 62 4 1 124 220 370 1.56e-25 100
MsG0780040726.01.T01 MTR_2g041510 40.260 154 60 4 2 124 193 345 4.04e-25 99.0
MsG0780040726.01.T01 MTR_2g041510 40.260 154 60 4 2 124 213 365 4.61e-25 99.0
MsG0780040726.01.T01 MTR_8g074640 37.086 151 66 4 2 124 201 350 2.89e-22 91.3
MsG0780040726.01.T01 MTR_5g079830 39.726 146 70 4 1 128 189 334 1.09e-21 89.7
MsG0780040726.01.T01 MTR_7g096760 46.809 94 18 3 25 86 1 94 5.47e-15 67.4
MsG0780040726.01.T01 MTR_4g125090 39.806 103 45 3 5 90 207 309 1.35e-13 66.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780040726.01.T01 AT2G26160 32.143 140 72 2 9 125 191 330 2.38e-18 80.1
MsG0780040726.01.T01 AT1G65770 34.752 141 66 3 9 124 193 332 2.73e-18 80.1
MsG0780040726.01.T01 AT4G35733 31.507 146 74 3 5 124 188 333 6.63e-18 79.0
MsG0780040726.01.T01 AT1G44080 33.562 146 70 4 5 124 173 317 2.12e-16 74.7
MsG0780040726.01.T01 AT1G65740 32.877 146 68 5 8 124 200 344 1.58e-14 69.7
MsG0780040726.01.T01 AT3G25750 30.435 138 75 3 9 125 180 317 4.94e-14 68.2
MsG0780040726.01.T01 AT3G25750 30.435 138 75 3 9 125 194 331 6.57e-14 67.8

Find 30 sgRNAs with CRISPR-Local

Find 39 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTAAACCTGCTGACAAATTT+TGG 0.080030 7:-81679092 MsG0780040726.01.T01:CDS
ACTTTATGTTGTGGATAAAA+TGG 0.170692 7:-81679517 MsG0780040726.01.T01:CDS
GTGCTGAAAATAGGCATATA+AGG 0.260640 7:-81679425 MsG0780040726.01.T01:CDS
TGTACCATTGTGCTGAAAAT+AGG 0.283196 7:-81679434 MsG0780040726.01.T01:CDS
GGGTGAGGTTTCGTTTGTTT+TGG 0.311852 7:-81679229 MsG0780040726.01.T01:CDS
TTGATCAAGAATGGGGTAAA+TGG 0.326226 7:-81679269 MsG0780040726.01.T01:CDS
ATGGTTAGAAGTAAAAGATT+TGG 0.332561 7:-81679250 MsG0780040726.01.T01:CDS
TGGTTAGAAGTAAAAGATTT+GGG 0.374795 7:-81679249 MsG0780040726.01.T01:CDS
CAATGCTCTTTCCTTGAAGT+TGG 0.381115 7:-81679478 MsG0780040726.01.T01:CDS
CAAAAGGGGTTACTTCTTGA+AGG 0.384442 7:-81679315 MsG0780040726.01.T01:intron
TTTATAAGCTTGATCAAGAA+TGG 0.392244 7:-81679278 MsG0780040726.01.T01:CDS
CTTTATGTTGTGGATAAAAT+GGG 0.396836 7:-81679516 MsG0780040726.01.T01:CDS
CCAATCCAAAATTTGTCAGC+AGG 0.420447 7:+81679087 None:intergenic
TGAAAATTGAACCAACTTCA+AGG 0.432491 7:+81679467 None:intergenic
TTGTTGGCTCGAGATTACTA+TGG 0.437431 7:-81679186 MsG0780040726.01.T01:CDS
TTATAAGCTTGATCAAGAAT+GGG 0.450966 7:-81679277 MsG0780040726.01.T01:CDS
TGATGATATAATACATTACA+AGG 0.467606 7:-81679544 MsG0780040726.01.T01:CDS
TCTACTTCTATTATGAAAGC+AGG 0.486961 7:-81679146 MsG0780040726.01.T01:CDS
TGCTGAAAATAGGCATATAA+GGG 0.494278 7:-81679424 MsG0780040726.01.T01:intron
CTTACAAATTAATTGATCAC+TGG 0.508472 7:+81679048 None:intergenic
ATCACTGGATGGAACAAAGT+TGG 0.510777 7:+81679063 None:intergenic
CAAGGGACAACTTTATGTTG+TGG 0.530035 7:-81679526 MsG0780040726.01.T01:CDS
TCATGTTAAAGGATGATTGT+GGG 0.537316 7:-81679582 None:intergenic
CAAATTAATTGATCACTGGA+TGG 0.540924 7:+81679052 None:intergenic
TGTTGGCTCGAGATTACTAT+GGG 0.552161 7:-81679185 MsG0780040726.01.T01:CDS
ATCATGTTAAAGGATGATTG+TGG 0.557247 7:-81679583 None:intergenic
AGAAGTAAAAGATTTGGGTG+AGG 0.564111 7:-81679244 MsG0780040726.01.T01:CDS
CTACTTCTATTATGAAAGCA+GGG 0.600724 7:-81679145 MsG0780040726.01.T01:CDS
TATAAGCTTGATCAAGAATG+GGG 0.627945 7:-81679276 MsG0780040726.01.T01:CDS
GATGATATAATACATTACAA+GGG 0.643392 7:-81679543 MsG0780040726.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTTATATATTAATGATGAA+AGG - Chr7:81679283-81679302 MsG0780040726.01.T01:CDS 10.0%
!! GATGATATAATACATTACAA+GGG - Chr7:81679083-81679102 MsG0780040726.01.T01:CDS 20.0%
!! TGATGATATAATACATTACA+AGG - Chr7:81679082-81679101 MsG0780040726.01.T01:CDS 20.0%
! ACTTTATGTTGTGGATAAAA+TGG - Chr7:81679109-81679128 MsG0780040726.01.T01:CDS 25.0%
! ATGGTTAGAAGTAAAAGATT+TGG - Chr7:81679376-81679395 MsG0780040726.01.T01:intron 25.0%
! CTTTATGTTGTGGATAAAAT+GGG - Chr7:81679110-81679129 MsG0780040726.01.T01:CDS 25.0%
! TGGTTAGAAGTAAAAGATTT+GGG - Chr7:81679377-81679396 MsG0780040726.01.T01:intron 25.0%
! TTATAAGCTTGATCAAGAAT+GGG - Chr7:81679349-81679368 MsG0780040726.01.T01:intron 25.0%
! TTTATAAGCTTGATCAAGAA+TGG - Chr7:81679348-81679367 MsG0780040726.01.T01:intron 25.0%
!! ATAAAATGGGAACAATTTTC+TGG - Chr7:81679123-81679142 MsG0780040726.01.T01:CDS 25.0%
!!! ATGATGAAAGGTATTACAAA+AGG - Chr7:81679295-81679314 MsG0780040726.01.T01:CDS 25.0%
!!! TGATGAAAGGTATTACAAAA+GGG - Chr7:81679296-81679315 MsG0780040726.01.T01:CDS 25.0%
AAGCAATTAGTAGAGTATGA+TGG - Chr7:81679245-81679264 MsG0780040726.01.T01:CDS 30.0%
CTACTTCTATTATGAAAGCA+GGG - Chr7:81679481-81679500 MsG0780040726.01.T01:CDS 30.0%
TATAAGCTTGATCAAGAATG+GGG - Chr7:81679350-81679369 MsG0780040726.01.T01:intron 30.0%
TCTACTTCTATTATGAAAGC+AGG - Chr7:81679480-81679499 MsG0780040726.01.T01:CDS 30.0%
TGAAAATTGAACCAACTTCA+AGG + Chr7:81679162-81679181 None:intergenic 30.0%
TGCTGAAAATAGGCATATAA+GGG - Chr7:81679202-81679221 MsG0780040726.01.T01:CDS 30.0%
! AGACACAAATTTTGCATTGT+TGG - Chr7:81679424-81679443 MsG0780040726.01.T01:intron 30.0%
!! GATGAAAGGTATTACAAAAG+GGG - Chr7:81679297-81679316 MsG0780040726.01.T01:CDS 30.0%
AGAAGTAAAAGATTTGGGTG+AGG - Chr7:81679382-81679401 MsG0780040726.01.T01:intron 35.0%
CAATTAGTAGAGTATGATGG+AGG - Chr7:81679248-81679267 MsG0780040726.01.T01:CDS 35.0%
GTGCTGAAAATAGGCATATA+AGG - Chr7:81679201-81679220 MsG0780040726.01.T01:CDS 35.0%
TGTACCATTGTGCTGAAAAT+AGG - Chr7:81679192-81679211 MsG0780040726.01.T01:CDS 35.0%
TTGATCAAGAATGGGGTAAA+TGG - Chr7:81679357-81679376 MsG0780040726.01.T01:intron 35.0%
! CTAAACCTGCTGACAAATTT+TGG - Chr7:81679534-81679553 MsG0780040726.01.T01:CDS 35.0%
! TATGCCTATTTTCAGCACAA+TGG + Chr7:81679199-81679218 None:intergenic 35.0%
!!! GCACAATGGTACATGTTTTT+TGG + Chr7:81679185-81679204 None:intergenic 35.0%
ATCACTGGATGGAACAAAGT+TGG + Chr7:81679566-81679585 None:intergenic 40.0%
CAAAAGGGGTTACTTCTTGA+AGG - Chr7:81679311-81679330 MsG0780040726.01.T01:CDS 40.0%
CAAGGGACAACTTTATGTTG+TGG - Chr7:81679100-81679119 MsG0780040726.01.T01:CDS 40.0%
CAATGCTCTTTCCTTGAAGT+TGG - Chr7:81679148-81679167 MsG0780040726.01.T01:CDS 40.0%
CCAATCCAAAATTTGTCAGC+AGG + Chr7:81679542-81679561 None:intergenic 40.0%
! CCTGCTGACAAATTTTGGAT+TGG - Chr7:81679539-81679558 MsG0780040726.01.T01:CDS 40.0%
!! ACTTCTTGAAGGCTGTTTTG+TGG - Chr7:81679322-81679341 MsG0780040726.01.T01:intron 40.0%
!! TGTTGGCTCGAGATTACTAT+GGG - Chr7:81679441-81679460 MsG0780040726.01.T01:CDS 40.0%
!! TTGTTGGCTCGAGATTACTA+TGG - Chr7:81679440-81679459 MsG0780040726.01.T01:CDS 40.0%
! GAGTATGATGGAGGTCTATG+TGG - Chr7:81679257-81679276 MsG0780040726.01.T01:CDS 45.0%
! GGGTGAGGTTTCGTTTGTTT+TGG - Chr7:81679397-81679416 MsG0780040726.01.T01:intron 45.0%
Chromosome Type Strat End Strand Name
Chr7 gene 81679056 81679592 81679056 ID=MsG0780040726.01;Name=MsG0780040726.01
Chr7 mRNA 81679056 81679592 81679056 ID=MsG0780040726.01.T01;Parent=MsG0780040726.01;Name=MsG0780040726.01.T01;_AED=0.50;_eAED=0.50;_QI=0|0|0|1|1|1|2|0|147
Chr7 exon 81679425 81679592 81679425 ID=MsG0780040726.01.T01:exon:38174;Parent=MsG0780040726.01.T01
Chr7 exon 81679056 81679331 81679056 ID=MsG0780040726.01.T01:exon:38173;Parent=MsG0780040726.01.T01
Chr7 CDS 81679425 81679592 81679425 ID=MsG0780040726.01.T01:cds;Parent=MsG0780040726.01.T01
Chr7 CDS 81679056 81679331 81679056 ID=MsG0780040726.01.T01:cds;Parent=MsG0780040726.01.T01
Gene Sequence

>MsG0780040726.01.T01

ATGATTGTGGGAGAAAACAAATATTTTGATGATATAATACATTACAAGGGACAACTTTATGTTGTGGATAAAATGGGAACAATTTTCTGGATCAATGCTCTTTCCTTGAAGTTGGTTCAATTTTCACCAAAAAACATGTACCATTGTGCTGAAAATAGGCATATAAGGGGGTTACTTCTTGAAGGCTGTTTTGTGGAAGTTTATAAGCTTGATCAAGAATGGGGTAAATGGTTAGAAGTAAAAGATTTGGGTGAGGTTTCGTTTGTTTTGGATAAAGACACAAATTTTGCATTGTTGGCTCGAGATTACTATGGGTGTGAAGAGAACTGCATCTACTTCTATTATGAAAGCAGGGCATCTTGTTTCAACTTGAAGAGTTTAGAGTCTAAACCTGCTGACAAATTTTGGATTGGTCCAACTTTGTTCCATCCAGTGATCAATTAA

Protein sequence

>MsG0780040726.01.T01

MIVGENKYFDDIIHYKGQLYVVDKMGTIFWINALSLKLVQFSPKNMYHCAENRHIRGLLLEGCFVEVYKLDQEWGKWLEVKDLGEVSFVLDKDTNFALLARDYYGCEENCIYFYYESRASCFNLKSLESKPADKFWIGPTLFHPVIN*