AlfalfaGEDB | MsG0480021728.01

M. sativa cultivar ZhongmuNo.1 / MsG0480021728.01

KEGG: K17867
GO: -
PFAM: PF00226 PF05207

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021728.01.T01	PNY05923.1	90.055	181	18	0	1	181	1	181	1.72E-114	333

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021728.01.T01	Q54CI5	29.891	184	86	5	10	178	14	169	1.12E-13	68.6

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021728.01.T01	A0A2K3NSB6	90.055	181	18	0	1	181	1	181	8.23e-115	333

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180002952.01	MsG0480021728.01	0.800537	1.375875e-48	6.422435e-46
MsG0180004357.01	MsG0480021728.01	0.821645	3.615765e-53	2.958760e-50
MsG0180004388.01	MsG0480021728.01	0.800857	1.183884e-48	5.571570e-46
MsG0280010614.01	MsG0480021728.01	0.802834	4.648971e-49	2.300530e-46
MsG0380013834.01	MsG0480021728.01	0.814220	1.721639e-51	1.148352e-48
MsG0380014573.01	MsG0480021728.01	0.802009	6.873726e-49	3.330456e-46
MsG0480020634.01	MsG0480021728.01	0.808652	2.788317e-50	1.603133e-47
MsG0480021728.01	MsG0480023707.01	0.812100	5.023500e-51	3.165005e-48
MsG0480021728.01	MsG0580026023.01	0.812949	3.277354e-51	2.111944e-48
MsG0480021728.01	MsG0680035692.01	0.801184	1.015141e-48	4.816946e-46
MsG0480021728.01	MsG0780038391.01	0.801204	1.005267e-48	4.772558e-46
MsG0480021728.01	MsG0780039252.01	0.802191	6.308158e-49	3.070522e-46
MsG0480021728.01	MsG0780040488.01	0.806474	8.082356e-50	4.391011e-47
MsG0480021728.01	MsG0880042698.01	0.809833	1.556012e-50	9.232108e-48
MsG0480021728.01	MsG0880044574.01	0.804763	1.848038e-49	9.609127e-47
MsG0380015677.01	MsG0480021728.01	0.806586	7.654777e-50	4.170462e-47
MsG0380018009.01	MsG0480021728.01	0.808903	2.463237e-50	1.425644e-47
MsG0280009748.01	MsG0480021728.01	0.824102	9.678824e-54	8.491285e-51
MsG0280010443.01	MsG0480021728.01	0.813510	2.468483e-51	1.615191e-48

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021728.01.T01	MTR_3g006810	82.320	181	29	2	1	181	1	178	3.46e-102	291
MsG0480021728.01.T01	MTR_6g003930	81.215	181	31	2	1	181	1	178	1.73e-100	287
MsG0480021728.01.T01	MTR_6g003930	81.215	181	31	2	1	181	73	250	3.70e-99	286
MsG0480021728.01.T01	MTR_4g074580	95.489	133	6	0	1	133	1	133	5.74e-90	259
MsG0480021728.01.T01	MTR_7g112360	28.030	132	85	4	12	137	17	144	7.73e-11	58.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021728.01.T01	AT4G10130	52.874	174	72	3	1	174	1	164	2.99e-59	183
MsG0480021728.01.T01	AT4G10130	52.874	174	72	3	1	174	1	164	2.99e-59	183
MsG0480021728.01.T01	AT4G10130	52.874	174	72	3	1	174	1	164	2.99e-59	183
MsG0480021728.01.T01	AT4G10130	52.874	174	72	3	1	174	1	164	2.99e-59	183
MsG0480021728.01.T01	AT4G10130	52.874	174	72	3	1	174	1	164	2.99e-59	183
MsG0480021728.01.T01	AT1G56300	27.273	132	80	4	7	129	9	133	2.38e-11	59.3
MsG0480021728.01.T01	AT3G13310	37.662	77	38	1	13	89	66	132	4.79e-11	58.5

Find 32 sgRNAs with CRISPR-Local

Find 36 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTGAAGTTGCAGAAGATTTA+AGG	0.106422	4:-63655576	MsG0480021728.01.T01:CDS
CTCATTGGAATTGCAGAAAA+TGG	0.178941	4:-63655470	MsG0480021728.01.T01:CDS
TGCAGAAGATTTAAGGTTAC+AGG	0.248624	4:-63655569	MsG0480021728.01.T01:CDS
TTCAAGGCGGGAGGATTTCT+TGG	0.279453	4:-63655602	MsG0480021728.01.T01:CDS
GATGTCTTTAATAGTTTATC+AGG	0.288420	4:+63655739	None:intergenic
GAAATCTCTCTGAAGTTGTT+TGG	0.379708	4:+63655698	None:intergenic
CTTAATGTTGATACTTTACC+AGG	0.381509	4:-63655403	MsG0480021728.01.T01:CDS
TCATTGGAATTGCAGAAAAT+GGG	0.406489	4:-63655469	MsG0480021728.01.T01:CDS
AGCTCGTCTTGTACTCGGTA+TGG	0.418652	4:-63655344	MsG0480021728.01.T01:CDS
TTCTCAAAGTTCACAAGGCA+TGG	0.443232	4:-63655678	MsG0480021728.01.T01:CDS
TTGAAAGCTCGTCTTGTACT+CGG	0.465157	4:-63655349	MsG0480021728.01.T01:CDS
CTCTCGTTTAATTTATGACA+AGG	0.490736	4:-63655635	MsG0480021728.01.T01:CDS
ACAAGGAACTGAAAATTTCA+AGG	0.503255	4:-63655618	MsG0480021728.01.T01:CDS
GCTCGTCTTGTACTCGGTAT+GGG	0.524137	4:-63655343	MsG0480021728.01.T01:CDS
CAAGGAAGAATCACTGATCC+TGG	0.528820	4:+63655385	None:intergenic
TCTCAAAGTTCACAAGGCAT+GGG	0.543905	4:-63655677	MsG0480021728.01.T01:CDS
GGAACTGAAAATTTCAAGGC+GGG	0.548219	4:-63655614	MsG0480021728.01.T01:CDS
TGAAGATGCTGGTGAAACAT+TGG	0.562066	4:-63655536	MsG0480021728.01.T01:CDS
CTTGTCATAAATTAAACGAG+AGG	0.568113	4:+63655636	None:intergenic
GGATCAGTGATTCTTCCTTG+TGG	0.587887	4:-63655382	MsG0480021728.01.T01:CDS
CAGCACTTCGGTAACTAGCA+CGG	0.609445	4:+63655776	None:intergenic
ACTGAAAATTTCAAGGCGGG+AGG	0.610139	4:-63655611	MsG0480021728.01.T01:CDS
CATTGGAATTGCAGAAAATG+GGG	0.618046	4:-63655468	MsG0480021728.01.T01:CDS
TTACGAGGTTCTCAATGTCA+AGG	0.619850	4:-63655821	MsG0480021728.01.T01:CDS
CATTGAGGAGACTCATTACG+AGG	0.636562	4:-63655836	MsG0480021728.01.T01:CDS
GAGATTTCTCAAAGTTCACA+AGG	0.644008	4:-63655683	MsG0480021728.01.T01:CDS
GTAAACTGAGTGCAGCACTT+CGG	0.645782	4:+63655764	None:intergenic
GCTGTCTTCATCTCTCAACA+AGG	0.680466	4:+63655441	None:intergenic
GATATGACAGTTGAAGATGC+TGG	0.682484	4:-63655547	MsG0480021728.01.T01:CDS
CGAGGTTCTCAATGTCAAGG+AGG	0.689102	4:-63655818	MsG0480021728.01.T01:CDS
AGGAACTGAAAATTTCAAGG+CGG	0.730120	4:-63655615	MsG0480021728.01.T01:CDS
TTCAACGAACAAGATCCACA+AGG	0.762477	4:+63655367	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	GATGTCTTTAATAGTTTATC+AGG	+	Chr4:63655453-63655472	None:intergenic	25.0%
	ACAAGGAACTGAAAATTTCA+AGG	-	Chr4:63655571-63655590	MsG0480021728.01.T01:CDS	30.0%
	CTTAATGTTGATACTTTACC+AGG	-	Chr4:63655786-63655805	MsG0480021728.01.T01:CDS	30.0%
	CTTGTCATAAATTAAACGAG+AGG	+	Chr4:63655556-63655575	None:intergenic	30.0%
	TCATTGGAATTGCAGAAAAT+GGG	-	Chr4:63655720-63655739	MsG0480021728.01.T01:CDS	30.0%
	TTTGGACAAGAAAAACATTG+AGG	-	Chr4:63655338-63655357	MsG0480021728.01.T01:CDS	30.0%
!	CTCTCGTTTAATTTATGACA+AGG	-	Chr4:63655554-63655573	MsG0480021728.01.T01:CDS	30.0%
!!	TTGTTTTATCAATGTCGATG+CGG	-	Chr4:63655678-63655697	MsG0480021728.01.T01:CDS	30.0%
	AGGAACTGAAAATTTCAAGG+CGG	-	Chr4:63655574-63655593	MsG0480021728.01.T01:CDS	35.0%
	CATTGGAATTGCAGAAAATG+GGG	-	Chr4:63655721-63655740	MsG0480021728.01.T01:CDS	35.0%
	CTCATTGGAATTGCAGAAAA+TGG	-	Chr4:63655719-63655738	MsG0480021728.01.T01:CDS	35.0%
	CTGAAGTTGCAGAAGATTTA+AGG	-	Chr4:63655613-63655632	MsG0480021728.01.T01:CDS	35.0%
	GAGATTTCTCAAAGTTCACA+AGG	-	Chr4:63655506-63655525	MsG0480021728.01.T01:CDS	35.0%
!	TGCAGAAGATTTAAGGTTAC+AGG	-	Chr4:63655620-63655639	MsG0480021728.01.T01:CDS	35.0%
!!	GAAATCTCTCTGAAGTTGTT+TGG	+	Chr4:63655494-63655513	None:intergenic	35.0%
!!	TTACTTTTCTGTGGACTCAT+TGG	-	Chr4:63655704-63655723	MsG0480021728.01.T01:CDS	35.0%
	GATATGACAGTTGAAGATGC+TGG	-	Chr4:63655642-63655661	MsG0480021728.01.T01:CDS	40.0%
	GGAACTGAAAATTTCAAGGC+GGG	-	Chr4:63655575-63655594	MsG0480021728.01.T01:CDS	40.0%
	TCTCAAAGTTCACAAGGCAT+GGG	-	Chr4:63655512-63655531	MsG0480021728.01.T01:CDS	40.0%
	TGAAGATGCTGGTGAAACAT+TGG	-	Chr4:63655653-63655672	MsG0480021728.01.T01:CDS	40.0%
	TTACGAGGTTCTCAATGTCA+AGG	-	Chr4:63655368-63655387	MsG0480021728.01.T01:CDS	40.0%
	TTCAACGAACAAGATCCACA+AGG	+	Chr4:63655825-63655844	None:intergenic	40.0%
	TTCTCAAAGTTCACAAGGCA+TGG	-	Chr4:63655511-63655530	MsG0480021728.01.T01:CDS	40.0%
	TTGAAAGCTCGTCTTGTACT+CGG	-	Chr4:63655840-63655859	MsG0480021728.01.T01:CDS	40.0%
!!	ATGCGGTGATTACTTTTCTG+TGG	-	Chr4:63655695-63655714	MsG0480021728.01.T01:CDS	40.0%
	ACTGAAAATTTCAAGGCGGG+AGG	-	Chr4:63655578-63655597	MsG0480021728.01.T01:CDS	45.0%
	CAAGGAAGAATCACTGATCC+TGG	+	Chr4:63655807-63655826	None:intergenic	45.0%
	CATTGAGGAGACTCATTACG+AGG	-	Chr4:63655353-63655372	MsG0480021728.01.T01:CDS	45.0%
	GCTGTCTTCATCTCTCAACA+AGG	+	Chr4:63655751-63655770	None:intergenic	45.0%
	GGATCAGTGATTCTTCCTTG+TGG	-	Chr4:63655807-63655826	MsG0480021728.01.T01:CDS	45.0%
	GTAAACTGAGTGCAGCACTT+CGG	+	Chr4:63655428-63655447	None:intergenic	45.0%
	AGCTCGTCTTGTACTCGGTA+TGG	-	Chr4:63655845-63655864	MsG0480021728.01.T01:CDS	50.0%
	CAGCACTTCGGTAACTAGCA+CGG	+	Chr4:63655416-63655435	None:intergenic	50.0%
	CGAGGTTCTCAATGTCAAGG+AGG	-	Chr4:63655371-63655390	MsG0480021728.01.T01:CDS	50.0%
	GCTCGTCTTGTACTCGGTAT+GGG	-	Chr4:63655846-63655865	MsG0480021728.01.T01:CDS	50.0%
	TTCAAGGCGGGAGGATTTCT+TGG	-	Chr4:63655587-63655606	MsG0480021728.01.T01:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
Chr4	gene	63655333	63655878	63655333	ID=MsG0480021728.01;Name=MsG0480021728.01
Chr4	mRNA	63655333	63655878	63655333	ID=MsG0480021728.01.T01;Parent=MsG0480021728.01;Name=MsG0480021728.01.T01;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|181
Chr4	exon	63655333	63655878	63655333	ID=MsG0480021728.01.T01:exon:3556;Parent=MsG0480021728.01.T01
Chr4	CDS	63655333	63655878	63655333	ID=MsG0480021728.01.T01:cds;Parent=MsG0480021728.01.T01

Gene Sequence

>MsG0480021728.01.T01

ATGATTTTGGACAAGAAAAACATTGAGGAGACTCATTACGAGGTTCTCAATGTCAAGGAGGATGCAAATTACGAAGAAATCCGTGCTAGTTACCGAAGTGCTGCACTCAGTTTACATCCTGATAAACTATTAAAGACATCTGATACATCTAGCAGTAACCAAACAACTTCAGAGAGATTTCTCAAAGTTCACAAGGCATGGGAAATTCTAAGCGATTCAACCTCTCGTTTAATTTATGACAAGGAACTGAAAATTTCAAGGCGGGAGGATTTCTTGGCTTCTGAAGTTGCAGAAGATTTAAGGTTACAGGATATGACAGTTGAAGATGCTGGTGAAACATTGGAATTGTTTTATCAATGTCGATGCGGTGATTACTTTTCTGTGGACTCATTGGAATTGCAGAAAATGGGGTATTCCTTGTTGAGAGATGAAGACAGCGTCAGCATTTCTATACTTAATGTTGATACTTTACCAGGATCAGTGATTCTTCCTTGTGGATCTTGTTCGTTGAAAGCTCGTCTTGTACTCGGTATGGGTAACAGTTGA

Protein sequence

>MsG0480021728.01.T01

MILDKKNIEETHYEVLNVKEDANYEEIRASYRSAALSLHPDKLLKTSDTSSSNQTTSERFLKVHKAWEILSDSTSRLIYDKELKISRREDFLASEVAEDLRLQDMTVEDAGETLELFYQCRCGDYFSVDSLELQKMGYSLLRDEDSVSISILNVDTLPGSVILPCGSCSLKARLVLGMGNS*