AlfalfaGEDB | MsG0480022693.01

M. sativa cultivar ZhongmuNo.1 / MsG0480022693.01

KEGG: K02929
GO: GO:0003735 GO:0005622 GO:0005840 GO:0006412
PFAM: PF00935

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480022693.01.T01	KAF4397433.1	99.048	105	1	0	1	105	50	154	2.20E-68	212

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480022693.01.T01	Q96499	97.143	105	3	0	1	105	1	105	4.33E-69	204

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480022693.01.T01	A0A7J6HQ78	99.048	105	1	0	1	105	50	154	1.05e-68	212

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048971.01	MsG0480022693.01	0.809403	1.925554e-50	1.129507e-47
MsG0180003612.01	MsG0480022693.01	0.800389	1.474940e-48	6.859162e-46
MsG0180003793.01	MsG0480022693.01	0.814807	1.276495e-51	8.650888e-49
MsG0180004129.01	MsG0480022693.01	0.827381	1.614149e-54	1.555961e-51
MsG0180004130.01	MsG0480022693.01	0.813845	2.082861e-51	1.375234e-48
MsG0180005366.01	MsG0480022693.01	0.808094	3.666636e-50	2.077619e-47
MsG0280010801.01	MsG0480022693.01	0.800541	1.373172e-48	6.410527e-46
MsG0480021159.01	MsG0480022693.01	0.816195	6.266902e-52	4.411351e-49
MsG0480022693.01	MsG0480023149.01	0.815529	8.825170e-52	6.099658e-49
MsG0480022693.01	MsG0480023467.01	0.854161	1.479730e-61	3.275349e-58
MsG0480022693.01	MsG0580024369.01	0.851322	9.568677e-61	1.929732e-57
MsG0480022693.01	MsG0580027043.01	0.824346	8.483614e-54	7.494102e-51
MsG0480022693.01	MsG0580029132.01	0.833939	3.996844e-56	4.673091e-53
MsG0480022693.01	MsG0680031763.01	0.801062	1.074697e-48	5.084205e-46
MsG0480022693.01	MsG0680035848.01	0.810697	1.013135e-50	6.149636e-48
MsG0480022693.01	MsG0780036822.01	0.806314	8.734373e-50	4.725807e-47
MsG0480022693.01	MsG0780041147.01	0.836630	8.363557e-57	1.059974e-53
MsG0480022693.01	MsG0780041223.01	0.816131	6.478346e-52	4.551988e-49
MsG0480022693.01	MsG0780041519.01	0.864111	1.538058e-64	4.773621e-61
MsG0480022693.01	MsG0880043517.01	0.833205	6.095659e-56	6.971144e-53
MsG0480022693.01	MsG0880046444.01	0.804392	2.207867e-49	1.137020e-46
MsG0480022693.01	MsG0880046879.01	0.811304	7.484517e-51	4.616411e-48
MsG0280006664.01	MsG0480022693.01	0.839215	1.810188e-57	2.482841e-54
MsG0280006819.01	MsG0480022693.01	0.813842	2.085525e-51	1.376909e-48
MsG0280006836.01	MsG0480022693.01	0.830704	2.528182e-55	2.684607e-52
MsG0280010495.01	MsG0480022693.01	0.825264	5.153077e-54	4.673885e-51

PPI

Gene1	Gene2	Type
MsG0480022693.01	MsG0280006424.01	PPI
MsG0480022693.01	MsG0180001372.01	PPI

Query id	Subject id	identity %	alignment length	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480022693.01.T01	MTR_7g100280	100.000	105	1	105	1	105	1.63e-71	208
MsG0480022693.01.T01	MTR_4g094308	100.000	105	1	105	1	105	1.63e-71	208
MsG0480022693.01.T01	MTR_2g008180	100.000	105	1	105	1	105	1.63e-71	208

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480022693.01.T01	AT4G14320	92.381	105	8	1	105	1	105	1.05e-66	196
MsG0480022693.01.T01	AT3G23390	92.381	105	8	1	105	1	105	1.05e-66	196
MsG0480022693.01.T01	AT4G14320	91.398	93	8	1	93	1	93	3.44e-57	174

Find 25 sgRNAs with CRISPR-Local

Find 55 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CAAAGTTACCCAATACAAAA+AGG	0.271604	4:+75550853	MsG0480022693.01.T01:CDS
TGTTTCACAACACGCAATTA+AGG	0.365888	4:+75551124	MsG0480022693.01.T01:CDS
CAAACCCGTCTTTCACAAAA+AGG	0.369511	4:+75550946	MsG0480022693.01.T01:CDS
AGGTGTGGTTTGAGATCGGC+TGG	0.393259	4:-75550636	None:intergenic
TAAAGATTCCATCGCTGCTC+AGG	0.399194	4:+75550877	MsG0480022693.01.T01:CDS
CGTTATGACCGCAAACAATC+TGG	0.415551	4:+75550908	MsG0480022693.01.T01:CDS
GTATTGGGTAACTTTGTGTA+AGG	0.453756	4:-75550846	None:intergenic
CACCATAACCAGATTGTTTG+CGG	0.454458	4:-75550916	None:intergenic
GACCGCAAACAATCTGGTTA+TGG	0.454865	4:+75550914	MsG0480022693.01.T01:CDS
AGGTGCAAGCACTTTGAGAT+TGG	0.462716	4:+75551236	MsG0480022693.01.T01:CDS
CCGATCTCAAACCACACCTC+TGG	0.506565	4:+75550640	MsG0480022693.01.T01:five_prime_UTR
TGAGATTGGTGGTGACAAGA+AGG	0.508837	4:+75551250	MsG0480022693.01.T01:CDS
AAACCACACCTCTGGAACAA+TGG	0.521072	4:+75550648	MsG0480022693.01.T01:exon
CCAGAGGTGTGGTTTGAGAT+CGG	0.530019	4:-75550640	None:intergenic
TTACCATTGTTCCAGAGGTG+TGG	0.538725	4:-75550651	None:intergenic
CGCAAACAATCTGGTTATGG+TGG	0.541862	4:+75550917	MsG0480022693.01.T01:CDS
GGTGGTGACAAGAAGGGAAA+AGG	0.543946	4:+75551257	MsG0480022693.01.T01:CDS
TGCAAGCACTTTGAGATTGG+TGG	0.545430	4:+75551239	MsG0480022693.01.T01:CDS
GAGATTGGTGGTGACAAGAA+GGG	0.555011	4:+75551251	MsG0480022693.01.T01:CDS
GGAGTTTACCATTGTTCCAG+AGG	0.592923	4:-75550656	None:intergenic
CTACTAAGAAAATTGTGTTG+AGG	0.599248	4:+75551078	MsG0480022693.01.T01:CDS
AAAGATTCCATCGCTGCTCA+GGG	0.607025	4:+75550878	MsG0480022693.01.T01:CDS
GAAAACCTACTGCAAAAGCA+AGG	0.613372	4:+75550808	MsG0480022693.01.T01:CDS
GTGTTGAGGCTTCAGTGTCA+AGG	0.618711	4:+75551092	MsG0480022693.01.T01:CDS
GGTGTGGTTTGAGATCGGCT+GGG	0.686032	4:-75550635	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AATTCAATTTTTGTTGTTAT+AGG	+	Chr4:75550766-75550785	MsG0480022693.01.T01:intron	15.0%
!!!	ATAAATGAAATTGTTGATAA+TGG	-	Chr4:75550718-75550737	None:intergenic	15.0%
!!!	TTTTCTAATTTTCTAATGAA+TGG	+	Chr4:75551391-75551410	MsG0480022693.01.T01:three_prime_UTR	15.0%
!!	CAAAATTAAAAACGAAAGAT+GGG	-	Chr4:75551346-75551365	None:intergenic	20.0%
!!!	AGTGAATTTTTACTGTTTTT+GGG	+	Chr4:75551175-75551194	MsG0480022693.01.T01:intron	20.0%
!	AAAGTTACCCAATACAAAAA+GGG	+	Chr4:75550854-75550873	MsG0480022693.01.T01:CDS	25.0%
!	GCAAAATTAAAAACGAAAGA+TGG	-	Chr4:75551347-75551366	None:intergenic	25.0%
!	TCAGATTAAGCAAAGATTTA+AGG	-	Chr4:75550987-75551006	None:intergenic	25.0%
!!	AATCTTTACCCTTTTTGTAT+TGG	-	Chr4:75550865-75550884	None:intergenic	25.0%
!!	ATCTTTACCCTTTTTGTATT+GGG	-	Chr4:75550864-75550883	None:intergenic	25.0%
!!	GAGTGAATTTTTACTGTTTT+TGG	+	Chr4:75551174-75551193	MsG0480022693.01.T01:intron	25.0%
	CAAAGTTACCCAATACAAAA+AGG	+	Chr4:75550853-75550872	MsG0480022693.01.T01:CDS	30.0%
	CTACTAAGAAAATTGTGTTG+AGG	+	Chr4:75551078-75551097	MsG0480022693.01.T01:CDS	30.0%
	TATCGTTGTGTGTTTGAATT+AGG	+	Chr4:75551049-75551068	MsG0480022693.01.T01:intron	30.0%
!	AAAAGGAACATCTCTGTTTT+AGG	+	Chr4:75551274-75551293	MsG0480022693.01.T01:CDS	30.0%
!	TAGTACCTTTTTGTGAAAGA+CGG	-	Chr4:75550954-75550973	None:intergenic	30.0%
	GTATTGGGTAACTTTGTGTA+AGG	-	Chr4:75550849-75550868	None:intergenic	35.0%
	TGTTTCACAACACGCAATTA+AGG	+	Chr4:75551124-75551143	MsG0480022693.01.T01:CDS	35.0%
!	AGTACCTTTTTGTGAAAGAC+GGG	-	Chr4:75550953-75550972	None:intergenic	35.0%
!	CAGTAGGTTTTCTTTGTCTT+TGG	-	Chr4:75550800-75550819	None:intergenic	35.0%
!!	GATAATGGATGAAAGTGTTG+TGG	-	Chr4:75550703-75550722	None:intergenic	35.0%
!!	GTTTTTGTTTGTGTTTGCAG+AGG	+	Chr4:75551216-75551235	MsG0480022693.01.T01:intron	35.0%
!!!	TTACTGTTTTTGGGTTCTGT+GGG	+	Chr4:75551184-75551203	MsG0480022693.01.T01:intron	35.0%
!!!	TTTACTGTTTTTGGGTTCTG+TGG	+	Chr4:75551183-75551202	MsG0480022693.01.T01:intron	35.0%
	AAGGTAAGATGAGGGAAATG+AGG	-	Chr4:75550680-75550699	None:intergenic	40.0%
	CAAACCCGTCTTTCACAAAA+AGG	+	Chr4:75550946-75550965	MsG0480022693.01.T01:CDS	40.0%
	CACCATAACCAGATTGTTTG+CGG	-	Chr4:75550919-75550938	None:intergenic	40.0%
	GAAAACCTACTGCAAAAGCA+AGG	+	Chr4:75550808-75550827	MsG0480022693.01.T01:CDS	40.0%
	TGTTGTGGAAGGTAAGATGA+GGG	-	Chr4:75550688-75550707	None:intergenic	40.0%
!!	AGGAACATCTCTGTTTTAGG+TGG	+	Chr4:75551277-75551296	MsG0480022693.01.T01:exon	40.0%
!!	ATGGATGAAAGTGTTGTGGA+AGG	-	Chr4:75550699-75550718	None:intergenic	40.0%
!!	CTTTTTGTGAAAGACGGGTT+TGG	-	Chr4:75550948-75550967	None:intergenic	40.0%
!!!	TTTTGGGTTCTGTGGGTAAT+GGG	+	Chr4:75551191-75551210	MsG0480022693.01.T01:intron	40.0%
!!!	TTTTTGGGTTCTGTGGGTAA+TGG	+	Chr4:75551190-75551209	MsG0480022693.01.T01:intron	40.0%
	AAAGATTCCATCGCTGCTCA+GGG	+	Chr4:75550878-75550897	MsG0480022693.01.T01:CDS	45.0%
	CGCAAACAATCTGGTTATGG+TGG	+	Chr4:75550917-75550936	MsG0480022693.01.T01:CDS	45.0%
	CGTTATGACCGCAAACAATC+TGG	+	Chr4:75550908-75550927	MsG0480022693.01.T01:CDS	45.0%
	GACCGCAAACAATCTGGTTA+TGG	+	Chr4:75550914-75550933	MsG0480022693.01.T01:CDS	45.0%
	GGAGTTTACCATTGTTCCAG+AGG	-	Chr4:75550659-75550678	None:intergenic	45.0%
	GTGTTGTGGAAGGTAAGATG+AGG	-	Chr4:75550689-75550708	None:intergenic	45.0%
	TAAAGATTCCATCGCTGCTC+AGG	+	Chr4:75550877-75550896	MsG0480022693.01.T01:CDS	45.0%
	TTACCATTGTTCCAGAGGTG+TGG	-	Chr4:75550654-75550673	None:intergenic	45.0%
!!	AAACCACACCTCTGGAACAA+TGG	+	Chr4:75550648-75550667	MsG0480022693.01.T01:exon	45.0%
!!	AGGTGCAAGCACTTTGAGAT+TGG	+	Chr4:75551236-75551255	MsG0480022693.01.T01:CDS	45.0%
!!	GAGATTGGTGGTGACAAGAA+GGG	+	Chr4:75551251-75551270	MsG0480022693.01.T01:CDS	45.0%
!!	TGAGATTGGTGGTGACAAGA+AGG	+	Chr4:75551250-75551269	MsG0480022693.01.T01:CDS	45.0%
!!	TGCAAGCACTTTGAGATTGG+TGG	+	Chr4:75551239-75551258	MsG0480022693.01.T01:CDS	45.0%
	GGTGGTGACAAGAAGGGAAA+AGG	+	Chr4:75551257-75551276	MsG0480022693.01.T01:CDS	50.0%
	GTGTTGAGGCTTCAGTGTCA+AGG	+	Chr4:75551092-75551111	MsG0480022693.01.T01:CDS	50.0%
!	CCAGAGGTGTGGTTTGAGAT+CGG	-	Chr4:75550643-75550662	None:intergenic	50.0%
!!	GCACTCCTTGCTTTTGCAGT+AGG	-	Chr4:75550816-75550835	None:intergenic	50.0%
	CCGATCTCAAACCACACCTC+TGG	+	Chr4:75550640-75550659	MsG0480022693.01.T01:five_prime_UTR	55.0%
!	ACGTTTTCCCTGAGCAGCGA+TGG	-	Chr4:75550888-75550907	None:intergenic	55.0%
!	AGGTGTGGTTTGAGATCGGC+TGG	-	Chr4:75550639-75550658	None:intergenic	55.0%
!	GGTGTGGTTTGAGATCGGCT+GGG	-	Chr4:75550638-75550657	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
Chr4	gene	75550630	75551428	75550630	ID=MsG0480022693.01;Name=MsG0480022693.01
Chr4	mRNA	75550630	75551428	75550630	ID=MsG0480022693.01.T01;Parent=MsG0480022693.01;Name=MsG0480022693.01.T01;_AED=0.50;_eAED=0.50;_QI=37\|1\|1\|1\|1\|1\|4\|133\|105
Chr4	exon	75550630	75550669	75550630	ID=MsG0480022693.01.T01:exon:10511;Parent=MsG0480022693.01.T01
Chr4	exon	75550788	75550967	75550788	ID=MsG0480022693.01.T01:exon:10512;Parent=MsG0480022693.01.T01
Chr4	exon	75551071	75551145	75551071	ID=MsG0480022693.01.T01:exon:10513;Parent=MsG0480022693.01.T01
Chr4	exon	75551236	75551428	75551236	ID=MsG0480022693.01.T01:exon:10514;Parent=MsG0480022693.01.T01
Chr4	five_prime_UTR	75550630	75550666	75550630	ID=MsG0480022693.01.T01:five_prime_utr;Parent=MsG0480022693.01.T01
Chr4	CDS	75550667	75550669	75550667	ID=MsG0480022693.01.T01:cds;Parent=MsG0480022693.01.T01
Chr4	CDS	75550788	75550967	75550788	ID=MsG0480022693.01.T01:cds;Parent=MsG0480022693.01.T01
Chr4	CDS	75551071	75551145	75551071	ID=MsG0480022693.01.T01:cds;Parent=MsG0480022693.01.T01
Chr4	CDS	75551236	75551295	75551236	ID=MsG0480022693.01.T01:cds;Parent=MsG0480022693.01.T01
Chr4	three_prime_UTR	75551296	75551428	75551296	ID=MsG0480022693.01.T01:three_prime_utr;Parent=MsG0480022693.01.T01

Gene Sequence	>MsG0480022693.01.T01 ATGGTGAACGTTCCAAAGACAAAGAAAACCTACTGCAAAAGCAAGGAGTGCAAGAAGCACACCTTACACAAAGTTACCCAATACAAAAAGGGTAAAGATTCCATCGCTGCTCAGGGAAAACGTCGTTATGACCGCAAACAATCTGGTTATGGTGGACAGACCAAACCCGTCTTTCACAAAAAGGCAAAAACTACTAAGAAAATTGTGTTGAGGCTTCAGTGTCAAGGTTGCAAGCATGTTTCACAACACGCAATTAAGAGGTGCAAGCACTTTGAGATTGGTGGTGACAAGAAGGGAAAAGGAACATCTCTGTTTTAG
Protein sequence	>MsG0480022693.01.T01 MVNVPKTKKTYCKSKECKKHTLHKVTQYKKGKDSIAAQGKRRYDRKQSGYGGQTKPVFHKKAKTTKKIVLRLQCQGCKHVSQHAIKRCKHFEIGGDKKGKGTSLF*