AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780041519.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780041519.01.T01 MTR_7g112880 97.170 106 3 0 4 109 31 136 6.66e-71 209
MsG0780041519.01.T01 MTR_7g108805 84.906 106 16 0 4 109 31 136 2.12e-62 188
MsG0780041519.01.T01 MTR_1g083460 83.019 106 18 0 4 109 31 136 7.70e-62 187
MsG0780041519.01.T01 MTR_7g112880 97.590 83 2 0 27 109 1 83 3.53e-54 166
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780041519.01.T01 AT5G27850 78.302 106 23 0 4 109 31 136 4.41e-59 180
MsG0780041519.01.T01 AT3G05590 76.415 106 25 0 4 109 31 136 1.59e-57 176
MsG0780041519.01.T01 AT2G47570 72.115 104 28 1 7 109 32 135 1.93e-50 158
MsG0780041519.01.T01 AT5G27850 75.904 83 20 0 27 109 1 83 1.76e-43 138
MsG0780041519.01.T01 AT3G05590 74.699 83 21 0 27 109 1 83 7.15e-43 137
MsG0780041519.01.T01 AT3G05590 74.699 83 21 0 27 109 1 83 7.15e-43 137

Find 33 sgRNAs with CRISPR-Local

Find 40 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCAACTTTGACAGAGAAAT+TGG 0.227766 7:+91941296 None:intergenic
GGTAAAATTGGTGTCGTTGT+GGG 0.314974 7:-91941141 MsG0780041519.01.T01:CDS
GGGCTGTAACAGATGATATC+AGG 0.365645 7:-91941119 MsG0780041519.01.T01:CDS
TATATCGCTTCCTTGTTAGA+AGG 0.375930 7:-91941382 MsG0780041519.01.T01:CDS
GTTATGCAGGACGGTAAAAT+TGG 0.388471 7:-91941153 MsG0780041519.01.T01:intron
TCTGTCCAAGGGGAGCTCTA+AGG 0.443443 7:+91940989 None:intergenic
GGGCAAGTTGATCAAATGTT+AGG 0.445307 7:+91941010 None:intergenic
CGGTAAAATTGGTGTCGTTG+TGG 0.446137 7:-91941142 MsG0780041519.01.T01:CDS
GTGCCGTCTCTGTGAACCTA+AGG 0.461512 7:+91941064 None:intergenic
CTGTCCAAGGGGAGCTCTAA+GGG 0.473811 7:+91940990 None:intergenic
GTAAAATTGGTGTCGTTGTG+GGG 0.476743 7:-91941140 MsG0780041519.01.T01:CDS
CTCATACCGTGTTCTGTCCA+AGG 0.524348 7:+91940977 None:intergenic
TGCCCTTAGGTTCACAGAGA+CGG 0.538036 7:-91941067 MsG0780041519.01.T01:CDS
AAAGTTGATCGAGTACATGA+AGG 0.551641 7:-91941282 MsG0780041519.01.T01:CDS
AAGTTGATCGAGTACATGAA+GGG 0.557741 7:-91941281 MsG0780041519.01.T01:CDS
CTTGCCCTTAGAGCTCCCCT+TGG 0.575267 7:-91940994 MsG0780041519.01.T01:CDS
TGCTGTCAGTCCTTCTAACA+AGG 0.579764 7:+91941372 None:intergenic
GGCTGTAACAGATGATATCA+GGG 0.589836 7:-91941118 MsG0780041519.01.T01:CDS
TCATACCGTGTTCTGTCCAA+GGG 0.595030 7:+91940978 None:intergenic
CAAGCGCTTGTTCATGAGCA+AGG 0.595633 7:-91941330 MsG0780041519.01.T01:CDS
AACTTTGACAGAGAAATTGG+AGG 0.595797 7:+91941299 None:intergenic
CGTGCAAGAATAGAGAAGGC+GGG 0.608166 7:-91941042 MsG0780041519.01.T01:CDS
TTGACAGAGAAATTGGAGGT+CGG 0.608358 7:+91941303 None:intergenic
AGCTCCCCTTGGACAGAACA+CGG 0.610780 7:-91940983 MsG0780041519.01.T01:CDS
TGCCGTCTCTGTGAACCTAA+GGG 0.611665 7:+91941065 None:intergenic
TAAAATTGGTGTCGTTGTGG+GGG 0.613763 7:-91941139 MsG0780041519.01.T01:CDS
GGCACGTGCAAGAATAGAGA+AGG 0.623253 7:-91941046 MsG0780041519.01.T01:CDS
AGTTGATCGAGTACATGAAG+GGG 0.649783 7:-91941280 MsG0780041519.01.T01:CDS
GATCGAGTACATGAAGGGGA+AGG 0.663524 7:-91941276 MsG0780041519.01.T01:intron
ACGTGCAAGAATAGAGAAGG+CGG 0.663924 7:-91941043 MsG0780041519.01.T01:CDS
GCAAGAATAGAGAAGGCGGG+TGG 0.710757 7:-91941039 MsG0780041519.01.T01:CDS
CATACCGTGTTCTGTCCAAG+GGG 0.721874 7:+91940979 None:intergenic
GACTGACAGCAATTTCAATG+CGG 0.745385 7:-91941360 MsG0780041519.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AACTAAACAATTATAATTTC+TGG + Chr7:91941157-91941176 None:intergenic 15.0%
!! ACTATGATCTAATTTTCTCA+TGG - Chr7:91941188-91941207 MsG0780041519.01.T01:intron 25.0%
!! CTATGATCTAATTTTCTCAT+GGG - Chr7:91941189-91941208 MsG0780041519.01.T01:intron 25.0%
ATCAACTTTGACAGAGAAAT+TGG + Chr7:91941080-91941099 None:intergenic 30.0%
TATTAAAAATTGCTGCCCTT+AGG - Chr7:91941293-91941312 MsG0780041519.01.T01:CDS 30.0%
AACTTTGACAGAGAAATTGG+AGG + Chr7:91941077-91941096 None:intergenic 35.0%
ATGGGATTATTGTGTTATGC+AGG - Chr7:91941207-91941226 MsG0780041519.01.T01:intron 35.0%
GATTATTGTGTTATGCAGGA+CGG - Chr7:91941211-91941230 MsG0780041519.01.T01:intron 35.0%
TATATCGCTTCCTTGTTAGA+AGG - Chr7:91940991-91941010 MsG0780041519.01.T01:CDS 35.0%
! AAAGTTGATCGAGTACATGA+AGG - Chr7:91941091-91941110 MsG0780041519.01.T01:CDS 35.0%
! AAGTTGATCGAGTACATGAA+GGG - Chr7:91941092-91941111 MsG0780041519.01.T01:CDS 35.0%
! AGGGCAGCAATTTTTAATAC+TGG + Chr7:91941292-91941311 None:intergenic 35.0%
! GGGCAGCAATTTTTAATACT+GGG + Chr7:91941291-91941310 None:intergenic 35.0%
AGTTGATCGAGTACATGAAG+GGG - Chr7:91941093-91941112 MsG0780041519.01.T01:CDS 40.0%
GACTGACAGCAATTTCAATG+CGG - Chr7:91941013-91941032 MsG0780041519.01.T01:CDS 40.0%
GGCTGTAACAGATGATATCA+GGG - Chr7:91941255-91941274 MsG0780041519.01.T01:intron 40.0%
GTTATGCAGGACGGTAAAAT+TGG - Chr7:91941220-91941239 MsG0780041519.01.T01:intron 40.0%
TTGACAGAGAAATTGGAGGT+CGG + Chr7:91941073-91941092 None:intergenic 40.0%
!! GGGCAAGTTGATCAAATGTT+AGG + Chr7:91941366-91941385 None:intergenic 40.0%
!! GGTAAAATTGGTGTCGTTGT+GGG - Chr7:91941232-91941251 MsG0780041519.01.T01:intron 40.0%
!! GTAAAATTGGTGTCGTTGTG+GGG - Chr7:91941233-91941252 MsG0780041519.01.T01:intron 40.0%
!! TAAAATTGGTGTCGTTGTGG+GGG - Chr7:91941234-91941253 MsG0780041519.01.T01:intron 40.0%
ACGTGCAAGAATAGAGAAGG+CGG - Chr7:91941330-91941349 MsG0780041519.01.T01:CDS 45.0%
GGGCTGTAACAGATGATATC+AGG - Chr7:91941254-91941273 MsG0780041519.01.T01:intron 45.0%
TCATACCGTGTTCTGTCCAA+GGG + Chr7:91941398-91941417 None:intergenic 45.0%
TGCTGTCAGTCCTTCTAACA+AGG + Chr7:91941004-91941023 None:intergenic 45.0%
!! CGGTAAAATTGGTGTCGTTG+TGG - Chr7:91941231-91941250 MsG0780041519.01.T01:intron 45.0%
CAAGCGCTTGTTCATGAGCA+AGG - Chr7:91941043-91941062 MsG0780041519.01.T01:CDS 50.0%
CATACCGTGTTCTGTCCAAG+GGG + Chr7:91941397-91941416 None:intergenic 50.0%
CGTGCAAGAATAGAGAAGGC+GGG - Chr7:91941331-91941350 MsG0780041519.01.T01:CDS 50.0%
GATCGAGTACATGAAGGGGA+AGG - Chr7:91941097-91941116 MsG0780041519.01.T01:CDS 50.0%
GGCACGTGCAAGAATAGAGA+AGG - Chr7:91941327-91941346 MsG0780041519.01.T01:CDS 50.0%
TGCCCTTAGGTTCACAGAGA+CGG - Chr7:91941306-91941325 MsG0780041519.01.T01:CDS 50.0%
TGCCGTCTCTGTGAACCTAA+GGG + Chr7:91941311-91941330 None:intergenic 50.0%
AGCTCCCCTTGGACAGAACA+CGG - Chr7:91941390-91941409 MsG0780041519.01.T01:CDS 55.0%
GCAAGAATAGAGAAGGCGGG+TGG - Chr7:91941334-91941353 MsG0780041519.01.T01:CDS 55.0%
GTGCCGTCTCTGTGAACCTA+AGG + Chr7:91941312-91941331 None:intergenic 55.0%
TCTGTCCAAGGGGAGCTCTA+AGG + Chr7:91941387-91941406 None:intergenic 55.0%
! CTGTCCAAGGGGAGCTCTAA+GGG + Chr7:91941386-91941405 None:intergenic 55.0%
CTTGCCCTTAGAGCTCCCCT+TGG - Chr7:91941379-91941398 MsG0780041519.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr7 gene 91940978 91941417 91940978 ID=MsG0780041519.01;Name=MsG0780041519.01
Chr7 mRNA 91940978 91941417 91940978 ID=MsG0780041519.01.T01;Parent=MsG0780041519.01;Name=MsG0780041519.01.T01;_AED=0.47;_eAED=0.48;_QI=0|1|0|1|1|1|2|0|109
Chr7 exon 91941277 91941417 91941277 ID=MsG0780041519.01.T01:exon:18825;Parent=MsG0780041519.01.T01
Chr7 exon 91940978 91941166 91940978 ID=MsG0780041519.01.T01:exon:18824;Parent=MsG0780041519.01.T01
Chr7 CDS 91941277 91941417 91941277 ID=MsG0780041519.01.T01:cds;Parent=MsG0780041519.01.T01
Chr7 CDS 91940978 91941166 91940978 ID=MsG0780041519.01.T01:cds;Parent=MsG0780041519.01.T01
Gene Sequence

>MsG0780041519.01.T01

TTGCACAATCAGCTATATCGCTTCCTTGTTAGAAGGACTGACAGCAATTTCAATGCGGTAATACTCAAGCGCTTGTTCATGAGCAAGGTCAACCGACCTCCAATTTCTCTGTCAAAGTTGATCGAGTACATGAAGGGGAAGGACGGTAAAATTGGTGTCGTTGTGGGGGCTGTAACAGATGATATCAGGGTAAATGAAGTCCCAGTATTAAAAATTGCTGCCCTTAGGTTCACAGAGACGGCACGTGCAAGAATAGAGAAGGCGGGTGGTGAATGCCTAACATTTGATCAACTTGCCCTTAGAGCTCCCCTTGGACAGAACACGGTATGA

Protein sequence

>MsG0780041519.01.T01

LHNQLYRFLVRRTDSNFNAVILKRLFMSKVNRPPISLSKLIEYMKGKDGKIGVVVGAVTDDIRVNEVPVLKIAALRFTETARARIEKAGGECLTFDQLALRAPLGQNTV*