AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180004129.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180004129.01.T01 MTR_1g075720 99.248 133 1 0 1 133 1 133 2.44e-90 258
MsG0180004129.01.T01 MTR_4g120770 95.489 133 6 0 1 133 1 133 4.62e-89 254
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180004129.01.T01 AT2G20450 84.962 133 20 0 1 133 1 133 7.83e-77 224
MsG0180004129.01.T01 AT4G27090 85.385 130 19 0 1 130 1 130 6.79e-76 221

Find 43 sgRNAs with CRISPR-Local

Find 96 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAACCTATCAAAATCATTA+AGG 0.237759 1:-73744319 None:intergenic
GATTATGTTGGCCAAGATTA+AGG 0.263288 1:+73744343 MsG0180004129.01.T01:CDS
GTCAACTATGGTCCAGATTA+TGG 0.285686 1:+73743848 MsG0180004129.01.T01:CDS
GAAAATGGGTTACCCTAGTT+TGG 0.329740 1:-73743901 None:intergenic
TTTGACGAGATCCTTCTTCT+TGG 0.330249 1:-73744096 None:intergenic
GAAAGCCTCTTGAAATTGAT+TGG 0.348542 1:-73744038 None:intergenic
TTGACGAGATCCTTCTTCTT+GGG 0.370770 1:-73744095 None:intergenic
ACAACGAGCTTGCCATAATC+TGG 0.375981 1:-73743860 None:intergenic
CTAAAATTATACCTTAATCT+TGG 0.381940 1:-73744354 None:intergenic
TCCAAGTTGTACTAAATAGA+AGG 0.385008 1:-73745146 None:intergenic
GTGCCTCAGAAAATCGAAAA+TGG 0.394255 1:+73743705 MsG0180004129.01.T01:exon
TGCAGAGGGCTGGTGTTGTT+AGG 0.431787 1:+73745045 MsG0180004129.01.T01:intron
TTGATGTTATCGACCAAACT+AGG 0.433748 1:+73743888 MsG0180004129.01.T01:CDS
GAACCTATCAAAATCATTAA+GGG 0.440003 1:-73744318 None:intergenic
AACAATCAATTTCCTTCCCC+AGG 0.448020 1:-73744279 None:intergenic
ATTGGAGCTCTCTCCATGTC+AGG 0.463357 1:-73744020 None:intergenic
AGAGTTGCACTTGTCAACTA+TGG 0.467898 1:+73743836 MsG0180004129.01.T01:CDS
GCTGAGGAAGACCGCTGCTT+AGG 0.472651 1:+73745082 MsG0180004129.01.T01:CDS
AATTCTACCTGCAGCTTCCA+TGG 0.474519 1:-73744120 None:intergenic
TTTCAATTTGTGCAGAGGGC+TGG 0.483986 1:+73745035 MsG0180004129.01.T01:intron
GGAAATTGATTGTTAAGAAG+AGG 0.493248 1:+73744288 MsG0180004129.01.T01:CDS
TTCAAGAGGTTTGTTGAGAT+CGG 0.494020 1:+73743812 MsG0180004129.01.T01:CDS
CAAGAGAGTTCCCAAGAAGA+AGG 0.508677 1:+73744085 MsG0180004129.01.T01:CDS
ACAAGTGGGAGAAGAGCTCC+TGG 0.539704 1:+73744261 MsG0180004129.01.T01:CDS
AATTGATTGTTAAGAAGAGG+AGG 0.539799 1:+73744291 MsG0180004129.01.T01:CDS
TGATAGGTTCAAGATTATGT+TGG 0.542816 1:+73744331 MsG0180004129.01.T01:CDS
TGATAGATGTGAAGAACAAG+TGG 0.562094 1:+73744246 MsG0180004129.01.T01:intron
GGCACTCGCAGCTGAAACAA+AGG 0.562800 1:-73743687 None:intergenic
GTCAAAGCCATGGAAGCTGC+AGG 0.563654 1:+73744113 MsG0180004129.01.T01:CDS
ATCTCAACAAACCTCTTGAA+CGG 0.571035 1:-73743809 None:intergenic
GAAGGATCTCGTCAAAGCCA+TGG 0.572972 1:+73744103 MsG0180004129.01.T01:CDS
GAGCTCCAATCAATTTCAAG+AGG 0.584468 1:+73744033 MsG0180004129.01.T01:CDS
ATCAAGCAAGAAAGAGCTAG+AGG 0.585153 1:+73745300 MsG0180004129.01.T01:three_prime_UTR
GGGAGAAGAGCTCCTGGGGA+AGG 0.586483 1:+73744267 MsG0180004129.01.T01:CDS
TCTTTGTTCAGCCGTTCAAG+AGG 0.591089 1:+73743798 MsG0180004129.01.T01:intron
TCTTGTTGATGCACCTGACA+TGG 0.596389 1:+73744007 MsG0180004129.01.T01:CDS
ATTGATTGTTAAGAAGAGGA+GGG 0.602431 1:+73744292 MsG0180004129.01.T01:CDS
TGATGTTATCGACCAAACTA+GGG 0.607546 1:+73743889 MsG0180004129.01.T01:CDS
GGCAAGAACTTGCTAAGCTG+AGG 0.613930 1:+73745066 MsG0180004129.01.T01:CDS
AAGTGGGAGAAGAGCTCCTG+GGG 0.619297 1:+73744263 MsG0180004129.01.T01:CDS
GATAGATGTGAAGAACAAGT+GGG 0.623335 1:+73744247 MsG0180004129.01.T01:intron
CAAGTGGGAGAAGAGCTCCT+GGG 0.641380 1:+73744262 MsG0180004129.01.T01:CDS
ATGAAGTAATGCCTAAGCAG+CGG 0.686386 1:-73745093 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATTGTATTTTTTTTTTTTG+TGG + Chr1:73744203-73744222 MsG0180004129.01.T01:intron 10.0%
!! ACAAAAATAAATACATAGAT+AGG - Chr1:73744157-73744176 None:intergenic 15.0%
!! TATGTTCAAAAATTGTTAAA+TGG - Chr1:73744868-73744887 None:intergenic 15.0%
!! TTTGGTTAAATAACTTAATT+AGG + Chr1:73744423-73744442 MsG0180004129.01.T01:intron 15.0%
!!! TTTTTTTTTTTTGTGGTAAT+TGG + Chr1:73744210-73744229 MsG0180004129.01.T01:intron 15.0%
!! AAAGATTGGAAATTTGAAAA+TGG - Chr1:73743919-73743938 None:intergenic 20.0%
!! AAGATTGGAAATTTGAAAAT+GGG - Chr1:73743918-73743937 None:intergenic 20.0%
!! ATTAGAGAAAGATTAAGAAA+TGG - Chr1:73743766-73743785 None:intergenic 20.0%
!! CTAAAATTATACCTTAATCT+TGG - Chr1:73744357-73744376 None:intergenic 20.0%
!! TTAAGTTATTTAACCAAACA+AGG - Chr1:73744421-73744440 None:intergenic 20.0%
!!! AATCTGTTTCTTAAGTTATT+TGG + Chr1:73744825-73744844 MsG0180004129.01.T01:intron 20.0%
!!! ATTGTTTTTGTATAAGCTTT+AGG + Chr1:73744607-73744626 MsG0180004129.01.T01:intron 20.0%
!!! GTTTGCATATTTTTTAATGT+TGG + Chr1:73745338-73745357 MsG0180004129.01.T01:three_prime_UTR 20.0%
!!! TGTTTTTTCAAATTGTGTTT+TGG + Chr1:73744974-73744993 MsG0180004129.01.T01:intron 20.0%
!!! TTGTTTTTGTATAAGCTTTA+GGG + Chr1:73744608-73744627 MsG0180004129.01.T01:intron 20.0%
!!! TTTTCAGAAATTAAGCAAAT+GGG - Chr1:73743959-73743978 None:intergenic 20.0%
!!! TTTTTCAGAAATTAAGCAAA+TGG - Chr1:73743960-73743979 None:intergenic 20.0%
! ATAATTTCAATTTGTGCAGA+GGG + Chr1:73745031-73745050 MsG0180004129.01.T01:intron 25.0%
! GAACCTATCAAAATCATTAA+GGG - Chr1:73744321-73744340 None:intergenic 25.0%
! TATGCAATCAAAAGCAAAAA+TGG - Chr1:73745261-73745280 None:intergenic 25.0%
! TGAACCTATCAAAATCATTA+AGG - Chr1:73744322-73744341 None:intergenic 25.0%
! TTTGGATAAATACCTTAACT+TGG + Chr1:73744505-73744524 MsG0180004129.01.T01:intron 25.0%
!! TTAAGGTATTTATCCAAACA+GGG - Chr1:73744503-73744522 None:intergenic 25.0%
!! TTTTCATAAGTCAATCCAAA+CGG - Chr1:73744635-73744654 None:intergenic 25.0%
!!! CTAGAGGTTTTATTGTTATT+AGG + Chr1:73745316-73745335 MsG0180004129.01.T01:three_prime_UTR 25.0%
!!! TTGTGAAATGCTTTTAAGTT+AGG + Chr1:73744477-73744496 MsG0180004129.01.T01:intron 25.0%
!!! TTGTGAAATGCTTTTTAGTT+AGG + Chr1:73744395-73744414 MsG0180004129.01.T01:intron 25.0%
AAAGTTGTATCTTCATAAGC+TGG + Chr1:73744710-73744729 MsG0180004129.01.T01:intron 30.0%
AATTGATTGTTAAGAAGAGG+AGG + Chr1:73744291-73744310 MsG0180004129.01.T01:CDS 30.0%
ATTGATTGTTAAGAAGAGGA+GGG + Chr1:73744292-73744311 MsG0180004129.01.T01:CDS 30.0%
CATAATTTCAATTTGTGCAG+AGG + Chr1:73745030-73745049 MsG0180004129.01.T01:intron 30.0%
GGAAATTGATTGTTAAGAAG+AGG + Chr1:73744288-73744307 MsG0180004129.01.T01:CDS 30.0%
GGATGAAAATGGAAAAAGAT+TGG - Chr1:73743933-73743952 None:intergenic 30.0%
TAATATTCATGAGCTTGTCA+AGG - Chr1:73744750-73744769 None:intergenic 30.0%
TCCAAGTTGTACTAAATAGA+AGG - Chr1:73745149-73745168 None:intergenic 30.0%
TCCTTCTATTTAGTACAACT+TGG + Chr1:73745145-73745164 MsG0180004129.01.T01:intron 30.0%
TCTCGTAACACAAATATCTT+AGG - Chr1:73745217-73745236 None:intergenic 30.0%
TGATAGGTTCAAGATTATGT+TGG + Chr1:73744331-73744350 MsG0180004129.01.T01:CDS 30.0%
! AATTAGGCTTTTAGTGTAAG+TGG + Chr1:73744439-73744458 MsG0180004129.01.T01:intron 30.0%
! GTTAAGGTATTTATCCAAAC+AGG - Chr1:73744504-73744523 None:intergenic 30.0%
! TTTTGTATCACTAAGACAGT+TGG + Chr1:73745178-73745197 MsG0180004129.01.T01:intron 30.0%
!!! TTGTGATTTGTTTGTTTTGC+AGG + Chr1:73743983-73744002 MsG0180004129.01.T01:intron 30.0%
AAACCCTATAAACTCAGCTA+AGG - Chr1:73744949-73744968 None:intergenic 35.0%
AACCCTATAAACTCAGCTAA+GGG - Chr1:73744948-73744967 None:intergenic 35.0%
ACACAATAAGCTCCAAGTTA+AGG - Chr1:73744520-73744539 None:intergenic 35.0%
ACCAACTTGAAACTTATGTG+TGG - Chr1:73744891-73744910 None:intergenic 35.0%
ATCTCAACAAACCTCTTGAA+CGG - Chr1:73743812-73743831 None:intergenic 35.0%
CAGATTAAGTCTGTTCAGAT+TGG - Chr1:73744811-73744830 None:intergenic 35.0%
GAAAGCCTCTTGAAATTGAT+TGG - Chr1:73744041-73744060 None:intergenic 35.0%
GATAGATGTGAAGAACAAGT+GGG + Chr1:73744247-73744266 MsG0180004129.01.T01:intron 35.0%
GATTATGTTGGCCAAGATTA+AGG + Chr1:73744343-73744362 MsG0180004129.01.T01:CDS 35.0%
GGTTATTATACGAGCTGTTA+TGG + Chr1:73744546-73744565 MsG0180004129.01.T01:intron 35.0%
TGATAGATGTGAAGAACAAG+TGG + Chr1:73744246-73744265 MsG0180004129.01.T01:intron 35.0%
TGATGTTATCGACCAAACTA+GGG + Chr1:73743889-73743908 MsG0180004129.01.T01:CDS 35.0%
TGGAGCTTATTGTGTAAATG+TGG + Chr1:73744525-73744544 MsG0180004129.01.T01:intron 35.0%
TTGATGTTATCGACCAAACT+AGG + Chr1:73743888-73743907 MsG0180004129.01.T01:CDS 35.0%
! ACCACACATAAGTTTCAAGT+TGG + Chr1:73744887-73744906 MsG0180004129.01.T01:intron 35.0%
! AGAAATTAAGCAAATGGGCT+AGG - Chr1:73743954-73743973 None:intergenic 35.0%
! TCACCATTTTCGATTTTCTG+AGG - Chr1:73743711-73743730 None:intergenic 35.0%
!! CTGCCCTTAATGATTTTGAT+AGG + Chr1:73744315-73744334 MsG0180004129.01.T01:CDS 35.0%
!! TTCAAGAGGTTTGTTGAGAT+CGG + Chr1:73743812-73743831 MsG0180004129.01.T01:CDS 35.0%
!!! CTTTTTAGTTAGGCCTTGTT+TGG + Chr1:73744405-73744424 MsG0180004129.01.T01:intron 35.0%
AACAATCAATTTCCTTCCCC+AGG - Chr1:73744282-73744301 None:intergenic 40.0%
AAGCCCTTAGCTGAGTTTAT+AGG + Chr1:73744942-73744961 MsG0180004129.01.T01:intron 40.0%
AGAGTTGCACTTGTCAACTA+TGG + Chr1:73743836-73743855 MsG0180004129.01.T01:CDS 40.0%
AGCCCTTAGCTGAGTTTATA+GGG + Chr1:73744943-73744962 MsG0180004129.01.T01:intron 40.0%
ATCAAGCAAGAAAGAGCTAG+AGG + Chr1:73745300-73745319 MsG0180004129.01.T01:three_prime_UTR 40.0%
ATGAAGTAATGCCTAAGCAG+CGG - Chr1:73745096-73745115 None:intergenic 40.0%
GAGCTCCAATCAATTTCAAG+AGG + Chr1:73744033-73744052 MsG0180004129.01.T01:CDS 40.0%
GTCAACTATGGTCCAGATTA+TGG + Chr1:73743848-73743867 MsG0180004129.01.T01:CDS 40.0%
GTGCCTCAGAAAATCGAAAA+TGG + Chr1:73743705-73743724 MsG0180004129.01.T01:exon 40.0%
TTGACGAGATCCTTCTTCTT+GGG - Chr1:73744098-73744117 None:intergenic 40.0%
TTTGACGAGATCCTTCTTCT+TGG - Chr1:73744099-73744118 None:intergenic 40.0%
!! CAAATGGGCTAGGATGAAAA+TGG - Chr1:73743944-73743963 None:intergenic 40.0%
!! GAAAATGGGTTACCCTAGTT+TGG - Chr1:73743904-73743923 None:intergenic 40.0%
!! TATAAGCTTTAGGGTCCGTT+TGG + Chr1:73744617-73744636 MsG0180004129.01.T01:intron 40.0%
!!! CTTTTAAGTTAGGCCCTGTT+TGG + Chr1:73744487-73744506 MsG0180004129.01.T01:intron 40.0%
AATTCTACCTGCAGCTTCCA+TGG - Chr1:73744123-73744142 None:intergenic 45.0%
ACAACGAGCTTGCCATAATC+TGG - Chr1:73743863-73743882 None:intergenic 45.0%
CAAGAGAGTTCCCAAGAAGA+AGG + Chr1:73744085-73744104 MsG0180004129.01.T01:CDS 45.0%
TCTTGTTGATGCACCTGACA+TGG + Chr1:73744007-73744026 MsG0180004129.01.T01:CDS 45.0%
TCTTTGTTCAGCCGTTCAAG+AGG + Chr1:73743798-73743817 MsG0180004129.01.T01:intron 45.0%
TTTCAATTTGTGCAGAGGGC+TGG + Chr1:73745035-73745054 MsG0180004129.01.T01:intron 45.0%
! AACTCAGCTAAGGGCTTGTT+TGG - Chr1:73744939-73744958 None:intergenic 45.0%
! AGCTAAGGGCTTGTTTGGAT+TGG - Chr1:73744934-73744953 None:intergenic 45.0%
ATTGGAGCTCTCTCCATGTC+AGG - Chr1:73744023-73744042 None:intergenic 50.0%
GAAGGATCTCGTCAAAGCCA+TGG + Chr1:73744103-73744122 MsG0180004129.01.T01:CDS 50.0%
!! GGCAAGAACTTGCTAAGCTG+AGG + Chr1:73745066-73745085 MsG0180004129.01.T01:CDS 50.0%
AAGTGGGAGAAGAGCTCCTG+GGG + Chr1:73744263-73744282 MsG0180004129.01.T01:CDS 55.0%
ACAAGTGGGAGAAGAGCTCC+TGG + Chr1:73744261-73744280 MsG0180004129.01.T01:CDS 55.0%
CAAGTGGGAGAAGAGCTCCT+GGG + Chr1:73744262-73744281 MsG0180004129.01.T01:CDS 55.0%
GGCACTCGCAGCTGAAACAA+AGG - Chr1:73743690-73743709 None:intergenic 55.0%
GTCAAAGCCATGGAAGCTGC+AGG + Chr1:73744113-73744132 MsG0180004129.01.T01:CDS 55.0%
!! TGCAGAGGGCTGGTGTTGTT+AGG + Chr1:73745045-73745064 MsG0180004129.01.T01:intron 55.0%
GCTGAGGAAGACCGCTGCTT+AGG + Chr1:73745082-73745101 MsG0180004129.01.T01:CDS 60.0%
GGGAGAAGAGCTCCTGGGGA+AGG + Chr1:73744267-73744286 MsG0180004129.01.T01:CDS 65.0%
Chromosome Type Strat End Strand Name
Chr1 gene 73743674 73745451 73743674 ID=MsG0180004129.01;Name=MsG0180004129.01
Chr1 mRNA 73743674 73745451 73743674 ID=MsG0180004129.01.T01;Parent=MsG0180004129.01;Name=MsG0180004129.01.T01;_AED=0.47;_eAED=0.47;_QI=50|1|1|1|0.4|0.5|6|272|133
Chr1 exon 73743674 73743726 73743674 ID=MsG0180004129.01.T01:exon:5355;Parent=MsG0180004129.01.T01
Chr1 exon 73743809 73743910 73743809 ID=MsG0180004129.01.T01:exon:5356;Parent=MsG0180004129.01.T01
Chr1 exon 73744005 73744134 73744005 ID=MsG0180004129.01.T01:exon:5357;Parent=MsG0180004129.01.T01
Chr1 exon 73744252 73744364 73744252 ID=MsG0180004129.01.T01:exon:5358;Parent=MsG0180004129.01.T01
Chr1 exon 73745050 73745156 73745050 ID=MsG0180004129.01.T01:exon:5359;Parent=MsG0180004129.01.T01
Chr1 exon 73745233 73745451 73745233 ID=MsG0180004129.01.T01:exon:5360;Parent=MsG0180004129.01.T01
Chr1 five_prime_UTR 73743674 73743723 73743674 ID=MsG0180004129.01.T01:five_prime_utr;Parent=MsG0180004129.01.T01
Chr1 CDS 73743724 73743726 73743724 ID=MsG0180004129.01.T01:cds;Parent=MsG0180004129.01.T01
Chr1 CDS 73743809 73743910 73743809 ID=MsG0180004129.01.T01:cds;Parent=MsG0180004129.01.T01
Chr1 CDS 73744005 73744134 73744005 ID=MsG0180004129.01.T01:cds;Parent=MsG0180004129.01.T01
Chr1 CDS 73744252 73744364 73744252 ID=MsG0180004129.01.T01:cds;Parent=MsG0180004129.01.T01
Chr1 CDS 73745050 73745103 73745050 ID=MsG0180004129.01.T01:cds;Parent=MsG0180004129.01.T01
Chr1 three_prime_UTR 73745104 73745156 73745104 ID=MsG0180004129.01.T01:three_prime_utr;Parent=MsG0180004129.01.T01
Chr1 three_prime_UTR 73745233 73745451 73745233 ID=MsG0180004129.01.T01:three_prime_utr;Parent=MsG0180004129.01.T01
Gene Sequence

>MsG0180004129.01.T01

ATGCCGTTCAAGAGGTTTGTTGAGATCGGAAGAGTTGCACTTGTCAACTATGGTCCAGATTATGGCAAGCTCGTTGTTATCGTTGATGTTATCGACCAAACTAGGGCTCTTGTTGATGCACCTGACATGGAGAGAGCTCCAATCAATTTCAAGAGGCTTTCTCTAACTGATATCAAGATTGATATCAAGAGAGTTCCCAAGAAGAAGGATCTCGTCAAAGCCATGGAAGCTGCAGATGTGAAGAACAAGTGGGAGAAGAGCTCCTGGGGAAGGAAATTGATTGTTAAGAAGAGGAGGGCTGCCCTTAATGATTTTGATAGGTTCAAGATTATGTTGGCCAAGATTAAGAGGGCTGGTGTTGTTAGGCAAGAACTTGCTAAGCTGAGGAAGACCGCTGCTTAG

Protein sequence

>MsG0180004129.01.T01

MPFKRFVEIGRVALVNYGPDYGKLVVIVDVIDQTRALVDAPDMERAPINFKRLSLTDIKIDIKRVPKKKDLVKAMEAADVKNKWEKSSWGRKLIVKKRRAALNDFDRFKIMLAKIKRAGVVRQELAKLRKTAA*