Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280006424.01.T01 | KAE8691450.1 | 96.296 | 108 | 4 | 0 | 2 | 109 | 33 | 140 | 1.87E-67 | 209 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280006424.01.T01 | Q96499 | 97.115 | 104 | 3 | 0 | 6 | 109 | 2 | 105 | 7.52E-68 | 201 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280006424.01.T01 | A0A6A2ZIL6 | 96.296 | 108 | 4 | 0 | 2 | 109 | 33 | 140 | 8.93e-68 | 209 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080047814.01 | MsG0280006424.01 | 0.817522 | 3.156463e-52 | 2.304029e-49 |
| MsG0080048135.01 | MsG0280006424.01 | 0.838839 | 2.265981e-57 | 3.072283e-54 |
| MsG0080048687.01 | MsG0280006424.01 | 0.858991 | 5.630833e-63 | 1.464393e-59 |
| MsG0080048971.01 | MsG0280006424.01 | 0.873961 | 9.795615e-68 | 4.331468e-64 |
| MsG0180000139.01 | MsG0280006424.01 | 0.826782 | 2.245890e-54 | 2.126935e-51 |
| MsG0180000339.01 | MsG0280006424.01 | 0.838424 | 2.899246e-57 | 3.881608e-54 |
| MsG0180000575.01 | MsG0280006424.01 | 0.885280 | 9.317556e-72 | 6.356643e-68 |
| MsG0180000614.01 | MsG0280006424.01 | 0.857622 | 1.439653e-62 | 3.575571e-59 |
| MsG0180000615.01 | MsG0280006424.01 | 0.876739 | 1.098427e-68 | 5.385061e-65 |
| MsG0180000743.01 | MsG0280006424.01 | 0.855725 | 5.201181e-62 | 1.212384e-58 |
| MsG0180000882.01 | MsG0280006424.01 | 0.825170 | 5.425005e-54 | 4.907491e-51 |
| MsG0180001102.01 | MsG0280006424.01 | 0.829637 | 4.604853e-55 | 4.740469e-52 |
| MsG0180003117.01 | MsG0280006424.01 | 0.813855 | 2.072240e-51 | 1.368610e-48 |
| MsG0180003313.01 | MsG0280006424.01 | 0.801416 | 9.096224e-49 | 4.341582e-46 |
| MsG0180003409.01 | MsG0280006424.01 | 0.812299 | 4.545656e-51 | 2.879481e-48 |
| MsG0180003612.01 | MsG0280006424.01 | 0.836681 | 8.113925e-57 | 1.030011e-53 |
| MsG0180003793.01 | MsG0280006424.01 | 0.867854 | 1.006649e-65 | 3.566241e-62 |
| MsG0180004009.01 | MsG0280006424.01 | 0.815734 | 7.942529e-52 | 5.520978e-49 |
| MsG0180004050.01 | MsG0280006424.01 | 0.840816 | 6.923119e-58 | 9.978072e-55 |
| MsG0180004129.01 | MsG0280006424.01 | 0.821008 | 5.071556e-53 | 4.077485e-50 |
| MsG0180004130.01 | MsG0280006424.01 | 0.827396 | 1.600738e-54 | 1.543686e-51 |
| MsG0180004152.01 | MsG0280006424.01 | 0.814560 | 1.448161e-51 | 9.749242e-49 |
| MsG0180004481.01 | MsG0280006424.01 | 0.831675 | 1.459421e-55 | 1.594962e-52 |
| MsG0180004482.01 | MsG0280006424.01 | 0.818614 | 1.787809e-52 | 1.344687e-49 |
| MsG0180004509.01 | MsG0280006424.01 | 0.881734 | 1.875533e-70 | 1.111474e-66 |
| MsG0180004625.01 | MsG0280006424.01 | 0.827856 | 1.241480e-54 | 1.213255e-51 |
| MsG0180004854.01 | MsG0280006424.01 | 0.860680 | 1.744452e-63 | 4.808856e-60 |
| MsG0180004991.01 | MsG0280006424.01 | 0.801343 | 9.415038e-49 | 4.485568e-46 |
| MsG0180005426.01 | MsG0280006424.01 | 0.855093 | 7.948368e-62 | 1.814010e-58 |
| MsG0180005659.01 | MsG0280006424.01 | 0.834352 | 3.149161e-56 | 3.727637e-53 |
| MsG0180005851.01 | MsG0280006424.01 | 0.801921 | 7.165612e-49 | 3.464135e-46 |
| MsG0180006013.01 | MsG0280006424.01 | 0.840679 | 7.519882e-58 | 1.079197e-54 |
| MsG0280006305.01 | MsG0280006424.01 | 0.831399 | 1.706541e-55 | 1.849696e-52 |
| MsG0280006406.01 | MsG0280006424.01 | 0.808354 | 3.226757e-50 | 1.841053e-47 |
| MsG0280006422.01 | MsG0280006424.01 | 0.857087 | 2.072522e-62 | 5.055099e-59 |
| MsG0280006424.01 | MsG0280006483.01 | 0.817001 | 4.135282e-52 | 2.975874e-49 |
| MsG0280006424.01 | MsG0280006993.01 | 0.830472 | 2.881591e-55 | 3.038759e-52 |
| MsG0280006424.01 | MsG0280007161.01 | 0.819292 | 1.253890e-52 | 9.609319e-50 |
| MsG0280006424.01 | MsG0280007222.01 | 0.812160 | 4.875304e-51 | 3.076737e-48 |
| MsG0280006424.01 | MsG0280007634.01 | 0.805149 | 1.534241e-49 | 8.057068e-47 |
| MsG0280006424.01 | MsG0280008118.01 | 0.828671 | 7.898791e-55 | 7.904203e-52 |
| MsG0280006424.01 | MsG0280008327.01 | 0.809561 | 1.780278e-50 | 1.048702e-47 |
| MsG0280006424.01 | MsG0280009111.01 | 0.850539 | 1.590889e-60 | 3.126333e-57 |
| MsG0280006424.01 | MsG0280009141.01 | 0.810420 | 1.163222e-50 | 7.008047e-48 |
| MsG0280006424.01 | MsG0280009372.01 | 0.815128 | 1.083353e-51 | 7.405661e-49 |
| MsG0280006424.01 | MsG0280009375.01 | 0.869093 | 4.007138e-66 | 1.484913e-62 |
| MsG0280006424.01 | MsG0280010383.01 | 0.883557 | 4.057095e-71 | 2.584885e-67 |
| MsG0280006424.01 | MsG0280010495.01 | 0.857665 | 1.398205e-62 | 3.477468e-59 |
| MsG0280006424.01 | MsG0280010525.01 | 0.809396 | 1.931554e-50 | 1.132845e-47 |
| MsG0280006424.01 | MsG0280010794.01 | 0.801402 | 9.158290e-49 | 4.369620e-46 |
| MsG0280006424.01 | MsG0280010801.01 | 0.851106 | 1.101585e-60 | 2.205647e-57 |
| MsG0280006424.01 | MsG0280011003.01 | 0.807032 | 6.160812e-50 | 3.395482e-47 |
| MsG0280006424.01 | MsG0280011013.01 | 0.802340 | 5.878228e-49 | 2.872312e-46 |
| MsG0280006424.01 | MsG0280011014.01 | 0.818843 | 1.585934e-52 | 1.200557e-49 |
| MsG0280006424.01 | MsG0280011388.01 | 0.829928 | 3.911788e-55 | 4.061198e-52 |
| MsG0280006424.01 | MsG0380012073.01 | 0.892087 | 2.192096e-74 | 1.973498e-70 |
| MsG0280006424.01 | MsG0380012471.01 | 0.811910 | 5.528184e-51 | 3.464837e-48 |
| MsG0280006424.01 | MsG0380012566.01 | 0.802682 | 4.997239e-49 | 2.463109e-46 |
| MsG0280006424.01 | MsG0380013178.01 | 0.802590 | 5.219095e-49 | 2.566512e-46 |
| MsG0280006424.01 | MsG0380014289.01 | 0.800436 | 1.442244e-48 | 6.715266e-46 |
| MsG0280006424.01 | MsG0380014539.01 | 0.805741 | 1.152885e-49 | 6.147475e-47 |
| MsG0280006424.01 | MsG0380014647.01 | 0.821851 | 3.240186e-53 | 2.667173e-50 |
| MsG0280006424.01 | MsG0380015027.01 | 0.804005 | 2.658994e-49 | 1.355838e-46 |
| MsG0280006424.01 | MsG0380015671.01 | 0.803392 | 3.562495e-49 | 1.788140e-46 |
| MsG0280006424.01 | MsG0380015793.01 | -0.813444 | 2.551553e-51 | 1.666626e-48 |
| MsG0280006424.01 | MsG0380015831.01 | 0.806424 | 8.281992e-50 | 4.493578e-47 |
| MsG0280006424.01 | MsG0380016067.01 | 0.842499 | 2.491862e-58 | 3.785413e-55 |
| MsG0280006424.01 | MsG0380016389.01 | 0.857659 | 1.404225e-62 | 3.491777e-59 |
| MsG0280006424.01 | MsG0380016733.01 | 0.802144 | 6.448013e-49 | 3.134982e-46 |
| MsG0280006424.01 | MsG0380016775.01 | 0.805744 | 1.151340e-49 | 6.139729e-47 |
| MsG0280006424.01 | MsG0380016860.01 | 0.857703 | 1.362613e-62 | 3.393132e-59 |
| MsG0280006424.01 | MsG0380017382.01 | 0.869329 | 3.360066e-66 | 1.256064e-62 |
| MsG0280006424.01 | MsG0480018084.01 | 0.833815 | 4.294447e-56 | 5.002019e-53 |
| MsG0280006424.01 | MsG0480018252.01 | 0.814290 | 1.660998e-51 | 1.110090e-48 |
| MsG0280006424.01 | MsG0480018461.01 | 0.810427 | 1.159237e-50 | 6.985335e-48 |
| MsG0280006424.01 | MsG0480018477.01 | 0.897352 | 1.528615e-76 | 1.720293e-72 |
| MsG0280006424.01 | MsG0480018894.01 | 0.833249 | 5.943488e-56 | 6.806197e-53 |
| MsG0280006424.01 | MsG0480020959.01 | 0.831110 | 2.009443e-55 | 2.159406e-52 |
| MsG0280006424.01 | MsG0480021006.01 | 0.844729 | 6.312593e-59 | 1.028521e-55 |
| MsG0280006424.01 | MsG0480021393.01 | 0.834172 | 3.494323e-56 | 4.113278e-53 |
| MsG0280006424.01 | MsG0480021448.01 | 0.857388 | 1.688850e-62 | 4.161147e-59 |
| MsG0280006424.01 | MsG0480021451.01 | 0.817880 | 2.621366e-52 | 1.932287e-49 |
| MsG0280006424.01 | MsG0480021453.01 | 0.857757 | 1.313157e-62 | 3.275784e-59 |
| MsG0280006424.01 | MsG0480021832.01 | 0.803937 | 2.746978e-49 | 1.398327e-46 |
| MsG0280006424.01 | MsG0480022164.01 | 0.828436 | 9.002484e-55 | 8.947725e-52 |
| MsG0280006424.01 | MsG0480022223.01 | 0.810643 | 1.040820e-50 | 6.308341e-48 |
| MsG0280006424.01 | MsG0480022696.01 | 0.823443 | 1.381354e-53 | 1.189516e-50 |
| MsG0280006424.01 | MsG0480022836.01 | 0.801309 | 9.567192e-49 | 4.554149e-46 |
| MsG0280006424.01 | MsG0480022960.01 | 0.802565 | 5.281894e-49 | 2.595625e-46 |
| MsG0280006424.01 | MsG0480022965.01 | 0.829103 | 6.206868e-55 | 6.290193e-52 |
| MsG0280006424.01 | MsG0480023149.01 | 0.871955 | 4.606154e-67 | 1.892765e-63 |
| MsG0280006424.01 | MsG0480023399.01 | 0.901120 | 3.693442e-78 | 4.917843e-74 |
| MsG0280006424.01 | MsG0480023467.01 | 0.829402 | 5.253692e-55 | 5.371593e-52 |
| MsG0280006424.01 | MsG0480023559.01 | 0.814563 | 1.445878e-51 | 9.734672e-49 |
| MsG0280006424.01 | MsG0480023771.01 | 0.814874 | 1.233735e-51 | 8.375687e-49 |
| MsG0280006424.01 | MsG0480023968.01 | 0.814000 | 1.925350e-51 | 1.276596e-48 |
| MsG0280006424.01 | MsG0580024036.01 | 0.806063 | 9.868979e-50 | 5.305677e-47 |
| MsG0280006424.01 | MsG0580024390.01 | 0.805365 | 1.382871e-49 | 7.302458e-47 |
| MsG0280006424.01 | MsG0580024754.01 | 0.834729 | 2.532657e-56 | 3.031328e-53 |
| MsG0280006424.01 | MsG0580025421.01 | 0.804753 | 1.857079e-49 | 9.653861e-47 |
| MsG0280006424.01 | MsG0580026059.01 | 0.804149 | 2.480792e-49 | 1.269662e-46 |
| MsG0280006424.01 | MsG0580026157.01 | 0.847159 | 1.379721e-59 | 2.430615e-56 |
| MsG0280006424.01 | MsG0580027043.01 | 0.859069 | 5.335679e-63 | 1.391176e-59 |
| MsG0280006424.01 | MsG0580029132.01 | 0.874136 | 8.547186e-68 | 3.803877e-64 |
| MsG0280006424.01 | MsG0680031507.01 | 0.842363 | 2.707644e-58 | 4.095937e-55 |
| MsG0280006424.01 | MsG0680031628.01 | 0.874752 | 5.282972e-68 | 2.405904e-64 |
| MsG0280006424.01 | MsG0680032770.01 | 0.920344 | 1.440530e-87 | 4.858422e-83 |
| MsG0280006424.01 | MsG0680034061.01 | 0.838024 | 3.676616e-57 | 4.863122e-54 |
| MsG0280006424.01 | MsG0680035848.01 | 0.857105 | 2.047843e-62 | 4.997667e-59 |
| MsG0280006424.01 | MsG0780036240.01 | 0.847004 | 1.521391e-59 | 2.666510e-56 |
| MsG0280006424.01 | MsG0780036547.01 | 0.836595 | 8.535075e-57 | 1.080632e-53 |
| MsG0280006424.01 | MsG0780036822.01 | 0.844079 | 9.437996e-59 | 1.506683e-55 |
| MsG0280006424.01 | MsG0780037240.01 | 0.857819 | 1.258888e-62 | 3.146908e-59 |
| MsG0280006424.01 | MsG0780038400.01 | 0.879292 | 1.403227e-69 | 7.567026e-66 |
| MsG0280006424.01 | MsG0780038587.01 | 0.864354 | 1.291680e-64 | 4.043578e-61 |
| MsG0280006424.01 | MsG0780038640.01 | 0.828064 | 1.106455e-54 | 1.087876e-51 |
| MsG0280006424.01 | MsG0780038645.01 | 0.840408 | 8.854159e-58 | 1.260088e-54 |
| MsG0280006424.01 | MsG0780039137.01 | 0.810976 | 8.817039e-51 | 5.392316e-48 |
| MsG0280006424.01 | MsG0780039844.01 | 0.807418 | 5.104969e-50 | 2.841906e-47 |
| MsG0280006424.01 | MsG0780040338.01 | 0.861956 | 7.124654e-64 | 2.051546e-60 |
| MsG0280006424.01 | MsG0780040782.01 | 0.829697 | 4.451504e-55 | 4.591071e-52 |
| MsG0280006424.01 | MsG0780040841.01 | 0.812188 | 4.807434e-51 | 3.036140e-48 |
| MsG0280006424.01 | MsG0780040845.01 | 0.815884 | 7.354878e-52 | 5.132861e-49 |
| MsG0280006424.01 | MsG0780040861.01 | 0.812287 | 4.574524e-51 | 2.896850e-48 |
| MsG0280006424.01 | MsG0780040900.01 | 0.904267 | 1.466461e-79 | 2.250938e-75 |
| MsG0280006424.01 | MsG0780041223.01 | 0.843174 | 1.648217e-58 | 2.557089e-55 |
| MsG0280006424.01 | MsG0780041224.01 | 0.801637 | 8.197521e-49 | 3.934422e-46 |
| MsG0280006424.01 | MsG0780041295.01 | 0.900120 | 1.006415e-77 | 1.282958e-73 |
| MsG0280006424.01 | MsG0780041423.01 | 0.804733 | 1.875173e-49 | 9.742972e-47 |
| MsG0280006424.01 | MsG0780041462.01 | 0.897761 | 1.027738e-76 | 1.178439e-72 |
| MsG0280006424.01 | MsG0780041490.01 | 0.803059 | 4.176781e-49 | 2.078501e-46 |
| MsG0280006424.01 | MsG0780041519.01 | 0.814180 | 1.756976e-51 | 1.170681e-48 |
| MsG0280006424.01 | MsG0780041559.01 | 0.842274 | 2.857264e-58 | 4.310006e-55 |
| MsG0280006424.01 | MsG0780041796.01 | 0.803419 | 3.517859e-49 | 1.766949e-46 |
| MsG0280006424.01 | MsG0880041967.01 | 0.859820 | 3.174618e-63 | 8.495482e-60 |
| MsG0280006424.01 | MsG0880042125.01 | 0.850792 | 1.350189e-60 | 2.675730e-57 |
| MsG0280006424.01 | MsG0880042519.01 | 0.803537 | 3.324589e-49 | 1.675019e-46 |
| MsG0280006424.01 | MsG0880042604.01 | 0.805296 | 1.429889e-49 | 7.537165e-47 |
| MsG0280006424.01 | MsG0880042779.01 | 0.850526 | 1.603708e-60 | 3.150242e-57 |
| MsG0280006424.01 | MsG0880043592.01 | 0.845695 | 3.459432e-59 | 5.813559e-56 |
| MsG0280006424.01 | MsG0880044711.01 | 0.829046 | 6.406656e-55 | 6.481455e-52 |
| MsG0280006424.01 | MsG0880045145.01 | 0.849132 | 3.933564e-60 | 7.385122e-57 |
| MsG0280006424.01 | MsG0880045631.01 | 0.816069 | 6.686725e-52 | 4.690759e-49 |
| MsG0280006424.01 | MsG0880046177.01 | 0.886083 | 4.653913e-72 | 3.276131e-68 |
| MsG0280006424.01 | MsG0880046444.01 | 0.907123 | 7.120555e-81 | 1.244921e-76 |
| MsG0280006424.01 | MsG0880046575.01 | 0.831290 | 1.815711e-55 | 1.961578e-52 |
| MsG0280006424.01 | MsG0880046631.01 | 0.833766 | 4.416855e-56 | 5.136965e-53 |
| MsG0280006424.01 | MsG0880046879.01 | 0.838225 | 3.262702e-57 | 4.341343e-54 |
| MsG0280006424.01 | MsG0880046933.01 | 0.878510 | 2.648773e-69 | 1.387038e-65 |
| MsG0280006424.01 | MsG0880047388.01 | 0.800618 | 1.324530e-48 | 6.195584e-46 |
| MsG0280006424.01 | MsG0880047427.01 | 0.827308 | 1.680607e-54 | 1.616413e-51 |
| MsG0280006424.01 | MsG0880047470.01 | 0.803258 | 3.797760e-49 | 1.899662e-46 |
| MsG0280006424.01 | MsG0880047607.01 | 0.827396 | 1.600738e-54 | 1.543686e-51 |
PPI
| Gene1 | Gene2 | Type |
|---|---|---|
| MsG0480022693.01 | MsG0280006424.01 | PPI |
| MsG0180001372.01 | MsG0280006424.01 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280006424.01.T01 | MTR_7g100280 | 100.000 | 104 | 0 | 0 | 6 | 109 | 2 | 105 | 1.81e-70 | 206 |
| MsG0280006424.01.T01 | MTR_4g094308 | 100.000 | 104 | 0 | 0 | 6 | 109 | 2 | 105 | 1.81e-70 | 206 |
| MsG0280006424.01.T01 | MTR_2g008180 | 100.000 | 104 | 0 | 0 | 6 | 109 | 2 | 105 | 1.81e-70 | 206 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280006424.01.T01 | AT4G14320 | 92.308 | 104 | 8 | 0 | 6 | 109 | 2 | 105 | 1.72e-65 | 193 |
| MsG0280006424.01.T01 | AT3G23390 | 92.308 | 104 | 8 | 0 | 6 | 109 | 2 | 105 | 1.72e-65 | 193 |
| MsG0280006424.01.T01 | AT4G14320 | 91.304 | 92 | 8 | 0 | 6 | 97 | 2 | 93 | 3.46e-56 | 171 |
Find 30 sgRNAs with CRISPR-Local
Find 67 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTCTTCTAGGTGGAGAATT+AGG | 0.245518 | 2:-1812091 | MsG0280006424.01.T01:exon |
| ATCTTTACCCTTCTTGTATT+GGG | 0.247979 | 2:+1812970 | None:intergenic |
| CCATTGTTGTTGAAATGTTT+TGG | 0.281419 | 2:-1812043 | MsG0280006424.01.T01:three_prime_UTR |
| TATCTTTACCCTTCTTGTAT+TGG | 0.312780 | 2:+1812969 | None:intergenic |
| CAAGGGAACTTCTCTCTTCT+AGG | 0.380957 | 2:-1812103 | MsG0280006424.01.T01:exon |
| GATCGCAAACAGTCTGGTTA+TGG | 0.387570 | 2:-1812917 | MsG0280006424.01.T01:CDS |
| AAAATCATCAAAGGAATTCA+AGG | 0.412835 | 2:+1811931 | None:intergenic |
| TGTTCTGAGGTTGCAATGCC+AGG | 0.414173 | 2:-1812313 | MsG0280006424.01.T01:CDS |
| GCATTCCTTGCTCTTGCAGT+AGG | 0.441918 | 2:+1813018 | None:intergenic |
| TGAGATTGGTGGTGATAAGA+AGG | 0.451732 | 2:-1812127 | MsG0280006424.01.T01:CDS |
| CGTTACGATCGCAAACAGTC+TGG | 0.464861 | 2:-1812923 | MsG0280006424.01.T01:CDS |
| TGTTTCTCAACATGCAATCA+AGG | 0.468902 | 2:-1812280 | MsG0280006424.01.T01:intron |
| GTTACCTTCTTGTGAAAGAC+GGG | 0.495280 | 2:+1812881 | None:intergenic |
| GAGAAACATGCTTGCAACCC+TGG | 0.514679 | 2:+1812295 | None:intergenic |
| GAGATTGGTGGTGATAAGAA+GGG | 0.526133 | 2:-1812126 | MsG0280006424.01.T01:CDS |
| CAAGGTTACCCAATACAAGA+AGG | 0.553515 | 2:-1812978 | MsG0280006424.01.T01:CDS |
| CGCAAACAGTCTGGTTATGG+TGG | 0.554816 | 2:-1812914 | MsG0280006424.01.T01:CDS |
| TGGTGGTGATAAGAAGGGCA+AGG | 0.556388 | 2:-1812121 | MsG0280006424.01.T01:CDS |
| CCAAAACATTTCAACAACAA+TGG | 0.558543 | 2:+1812043 | None:intergenic |
| AAAGATAGCATCGCCGCTCA+AGG | 0.571046 | 2:-1812953 | MsG0280006424.01.T01:CDS |
| AGTTACCTTCTTGTGAAAGA+CGG | 0.572609 | 2:+1812880 | None:intergenic |
| CAAGAAGCATACACTTCACA+AGG | 0.596444 | 2:-1812996 | MsG0280006424.01.T01:CDS |
| GAAAACCTACTGCAAGAGCA+AGG | 0.601503 | 2:-1813023 | MsG0280006424.01.T01:CDS |
| CCACTAAGAAAATTGTTCTG+AGG | 0.602363 | 2:-1812326 | MsG0280006424.01.T01:CDS |
| TAAGCCCGTCTTTCACAAGA+AGG | 0.606865 | 2:-1812885 | MsG0280006424.01.T01:intron |
| AAGGTTACCCAATACAAGAA+GGG | 0.630634 | 2:-1812977 | MsG0280006424.01.T01:CDS |
| GGTGGTGATAAGAAGGGCAA+GGG | 0.630826 | 2:-1812120 | MsG0280006424.01.T01:CDS |
| GTAACGACGCTTACCTTGAG+CGG | 0.638526 | 2:+1812940 | None:intergenic |
| GTTCTGAGGTTGCAATGCCA+GGG | 0.641034 | 2:-1812312 | MsG0280006424.01.T01:CDS |
| GGGAACTTCTCTCTTCTAGG+TGG | 0.678633 | 2:-1812100 | MsG0280006424.01.T01:exon |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTAAAAATCTTTTATCATTT+GGG | - | Chr2:1813019-1813038 | MsG0280006424.01.T01:CDS | 10.0% |
| !!! | TTTAAAAATCTTTTATCATT+TGG | - | Chr2:1813018-1813037 | MsG0280006424.01.T01:CDS | 10.0% |
| !!! | AAGATTTTTAAAATCATCAA+AGG | + | Chr2:1813010-1813029 | None:intergenic | 15.0% |
| !!! | ATCTAATTTTATCATAACAA+TGG | + | Chr2:1813060-1813079 | None:intergenic | 15.0% |
| !!! | ATTTAATTTTTGTTTGTGTT+TGG | - | Chr2:1812560-1812579 | MsG0280006424.01.T01:intron | 15.0% |
| !!! | GATTTTTTTTTTTAGTTGAA+TGG | - | Chr2:1812530-1812549 | MsG0280006424.01.T01:intron | 15.0% |
| !! | AAATGTATAAAAGTAATGAG+GGG | + | Chr2:1812086-1812105 | None:intergenic | 20.0% |
| !! | CTTACAATCAACTAAAAAAA+TGG | + | Chr2:1812130-1812149 | None:intergenic | 20.0% |
| !! | GAAATGTATAAAAGTAATGA+GGG | + | Chr2:1812087-1812106 | None:intergenic | 20.0% |
| !! | GAATGCAAAAAATTCAAATT+TGG | - | Chr2:1812453-1812472 | MsG0280006424.01.T01:intron | 20.0% |
| !! | TGAAATGTATAAAAGTAATG+AGG | + | Chr2:1812088-1812107 | None:intergenic | 20.0% |
| !!! | CAGAAATTTTATTTTGTAGA+TGG | - | Chr2:1812711-1812730 | MsG0280006424.01.T01:intron | 20.0% |
| !!! | TTTTATTTTGTAGATGGATA+TGG | - | Chr2:1812717-1812736 | MsG0280006424.01.T01:intron | 20.0% |
| ! | AAAATCATCAAAGGAATTCA+AGG | + | Chr2:1813001-1813020 | None:intergenic | 25.0% |
| ! | AATGTATAAAAGTAATGAGG+GGG | + | Chr2:1812085-1812104 | None:intergenic | 25.0% |
| ! | ATATGGTTGTTTGTATCTAA+TGG | - | Chr2:1812734-1812753 | MsG0280006424.01.T01:intron | 25.0% |
| ! | TGGATTCAAATATGGTTAAT+AGG | - | Chr2:1812254-1812273 | MsG0280006424.01.T01:intron | 25.0% |
| !!! | TAGCTTTATGCAAATGATTA+GGG | - | Chr2:1812211-1812230 | MsG0280006424.01.T01:intron | 25.0% |
| !!! | TGTGTTTGGTTTTATTGTAT+AGG | - | Chr2:1812574-1812593 | MsG0280006424.01.T01:intron | 25.0% |
| !!! | TTAGCTTTATGCAAATGATT+AGG | - | Chr2:1812210-1812229 | MsG0280006424.01.T01:intron | 25.0% |
| ATCTTTACCCTTCTTGTATT+GGG | + | Chr2:1811962-1811981 | None:intergenic | 30.0% | |
| CCAAAACATTTCAACAACAA+TGG | + | Chr2:1812889-1812908 | None:intergenic | 30.0% | |
| GGTTAATAGGAAGAACATTA+TGG | - | Chr2:1812267-1812286 | MsG0280006424.01.T01:intron | 30.0% | |
| TATCTTTACCCTTCTTGTAT+TGG | + | Chr2:1811963-1811982 | None:intergenic | 30.0% | |
| ! | TTTTGAGCTGAATGTGAAAT+TGG | - | Chr2:1812366-1812385 | MsG0280006424.01.T01:intron | 30.0% |
| !! | CCATTGTTGTTGAAATGTTT+TGG | - | Chr2:1812886-1812905 | MsG0280006424.01.T01:CDS | 30.0% |
| !!! | TGAAATGTTTTGGTTCTCTA+TGG | - | Chr2:1812896-1812915 | MsG0280006424.01.T01:CDS | 30.0% |
| AAGGTTACCCAATACAAGAA+GGG | - | Chr2:1811952-1811971 | MsG0280006424.01.T01:three_prime_UTR | 35.0% | |
| AGGGAAGATTTAGAGATTGA+TGG | - | Chr2:1812230-1812249 | MsG0280006424.01.T01:intron | 35.0% | |
| AGTTACCTTCTTGTGAAAGA+CGG | + | Chr2:1812052-1812071 | None:intergenic | 35.0% | |
| CAAACCCACTTAGTGATATA+AGG | - | Chr2:1812310-1812329 | MsG0280006424.01.T01:CDS | 35.0% | |
| CCACTAAGAAAATTGTTCTG+AGG | - | Chr2:1812603-1812622 | MsG0280006424.01.T01:intron | 35.0% | |
| TGTTTCTCAACATGCAATCA+AGG | - | Chr2:1812649-1812668 | MsG0280006424.01.T01:intron | 35.0% | |
| ! | AAGATTTAGAGATTGATGGC+TGG | - | Chr2:1812234-1812253 | MsG0280006424.01.T01:intron | 35.0% |
| ! | CAAGCCTTATATCACTAAGT+GGG | + | Chr2:1812317-1812336 | None:intergenic | 35.0% |
| ! | CAGTAGGTTTTCTTTGTCTT+TGG | + | Chr2:1811898-1811917 | None:intergenic | 35.0% |
| ! | CCTCAGAACAATTTTCTTAG+TGG | + | Chr2:1812606-1812625 | None:intergenic | 35.0% |
| ! | CTTATATCACTAAGTGGGTT+TGG | + | Chr2:1812312-1812331 | None:intergenic | 35.0% |
| ! | TTGATGGCTGGATTCAAATA+TGG | - | Chr2:1812246-1812265 | MsG0280006424.01.T01:intron | 35.0% |
| CAAGGTTACCCAATACAAGA+AGG | - | Chr2:1811951-1811970 | MsG0280006424.01.T01:three_prime_UTR | 40.0% | |
| CCACTTAGTGATATAAGGCT+TGG | - | Chr2:1812315-1812334 | MsG0280006424.01.T01:CDS | 40.0% | |
| GTTACCTTCTTGTGAAAGAC+GGG | + | Chr2:1812051-1812070 | None:intergenic | 40.0% | |
| TCTCTTCTAGGTGGAGAATT+AGG | - | Chr2:1812838-1812857 | MsG0280006424.01.T01:intron | 40.0% | |
| ! | CAAGAAGCATACACTTCACA+AGG | - | Chr2:1811933-1811952 | MsG0280006424.01.T01:three_prime_UTR | 40.0% |
| ! | CCAAGCCTTATATCACTAAG+TGG | + | Chr2:1812318-1812337 | None:intergenic | 40.0% |
| ! | GTTGTGTTTCTGTTATGCAG+AGG | - | Chr2:1812768-1812787 | MsG0280006424.01.T01:intron | 40.0% |
| !! | GAGATTGGTGGTGATAAGAA+GGG | - | Chr2:1812803-1812822 | MsG0280006424.01.T01:intron | 40.0% |
| !! | TGAGATTGGTGGTGATAAGA+AGG | - | Chr2:1812802-1812821 | MsG0280006424.01.T01:intron | 40.0% |
| !!! | AGGTGCAAGCATTTTGAGAT+TGG | - | Chr2:1812788-1812807 | MsG0280006424.01.T01:intron | 40.0% |
| !!! | TGCAAGCATTTTGAGATTGG+TGG | - | Chr2:1812791-1812810 | MsG0280006424.01.T01:intron | 40.0% |
| AAAGTAATGAGGGGGTGAAG+TGG | + | Chr2:1812077-1812096 | None:intergenic | 45.0% | |
| AAGTAATGAGGGGGTGAAGT+GGG | + | Chr2:1812076-1812095 | None:intergenic | 45.0% | |
| CAAGGGAACTTCTCTCTTCT+AGG | - | Chr2:1812826-1812845 | MsG0280006424.01.T01:intron | 45.0% | |
| GAAAACCTACTGCAAGAGCA+AGG | - | Chr2:1811906-1811925 | MsG0280006424.01.T01:three_prime_UTR | 45.0% | |
| GATCGCAAACAGTCTGGTTA+TGG | - | Chr2:1812012-1812031 | MsG0280006424.01.T01:three_prime_UTR | 45.0% | |
| TAAGCCCGTCTTTCACAAGA+AGG | - | Chr2:1812044-1812063 | MsG0280006424.01.T01:three_prime_UTR | 45.0% | |
| AAAGATAGCATCGCCGCTCA+AGG | - | Chr2:1811976-1811995 | MsG0280006424.01.T01:three_prime_UTR | 50.0% | |
| CGCAAACAGTCTGGTTATGG+TGG | - | Chr2:1812015-1812034 | MsG0280006424.01.T01:three_prime_UTR | 50.0% | |
| CGTTACGATCGCAAACAGTC+TGG | - | Chr2:1812006-1812025 | MsG0280006424.01.T01:three_prime_UTR | 50.0% | |
| GAGAAACATGCTTGCAACCC+TGG | + | Chr2:1812637-1812656 | None:intergenic | 50.0% | |
| GCATTCCTTGCTCTTGCAGT+AGG | + | Chr2:1811914-1811933 | None:intergenic | 50.0% | |
| GGGAACTTCTCTCTTCTAGG+TGG | - | Chr2:1812829-1812848 | MsG0280006424.01.T01:intron | 50.0% | |
| GGTGGTGATAAGAAGGGCAA+GGG | - | Chr2:1812809-1812828 | MsG0280006424.01.T01:intron | 50.0% | |
| GTAACGACGCTTACCTTGAG+CGG | + | Chr2:1811992-1812011 | None:intergenic | 50.0% | |
| ! | TGGTGGTGATAAGAAGGGCA+AGG | - | Chr2:1812808-1812827 | MsG0280006424.01.T01:intron | 50.0% |
| !! | GTTCTGAGGTTGCAATGCCA+GGG | - | Chr2:1812617-1812636 | MsG0280006424.01.T01:intron | 50.0% |
| !! | TGTTCTGAGGTTGCAATGCC+AGG | - | Chr2:1812616-1812635 | MsG0280006424.01.T01:intron | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr2 | gene | 1811871 | 1813080 | 1811871 | ID=MsG0280006424.01;Name=MsG0280006424.01 |
| Chr2 | mRNA | 1811871 | 1813080 | 1811871 | ID=MsG0280006424.01.T01;Parent=MsG0280006424.01;Name=MsG0280006424.01.T01;_AED=0.48;_eAED=0.49;_QI=0|1|0.66|1|1|1|3|233|109 |
| Chr2 | exon | 1811871 | 1812163 | 1811871 | ID=MsG0280006424.01.T01:exon:1217;Parent=MsG0280006424.01.T01 |
| Chr2 | exon | 1812281 | 1812355 | 1812281 | ID=MsG0280006424.01.T01:exon:1216;Parent=MsG0280006424.01.T01 |
| Chr2 | exon | 1812886 | 1813080 | 1812886 | ID=MsG0280006424.01.T01:exon:1215;Parent=MsG0280006424.01.T01 |
| Chr2 | CDS | 1812886 | 1813080 | 1812886 | ID=MsG0280006424.01.T01:cds;Parent=MsG0280006424.01.T01 |
| Chr2 | CDS | 1812281 | 1812355 | 1812281 | ID=MsG0280006424.01.T01:cds;Parent=MsG0280006424.01.T01 |
| Chr2 | CDS | 1812104 | 1812163 | 1812104 | ID=MsG0280006424.01.T01:cds;Parent=MsG0280006424.01.T01 |
| Chr2 | three_prime_UTR | 1811871 | 1812103 | 1811871 | ID=MsG0280006424.01.T01:three_prime_utr;Parent=MsG0280006424.01.T01 |
| Gene Sequence |
| Protein sequence |
