AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780036822.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780036822.01.T01 MTR_7g021030 98.551 69 1 0 1 69 1 69 1.38e-43 138
MsG0780036822.01.T01 MTR_7g021030 98.551 69 1 0 1 69 1 69 4.94e-43 137
MsG0780036822.01.T01 MTR_0024s0220 95.652 69 3 0 1 69 1 69 3.78e-42 135
MsG0780036822.01.T01 MTR_0024s0220 95.652 69 3 0 1 69 1 69 5.06e-42 135
MsG0780036822.01.T01 MTR_5g088740 94.203 69 4 0 1 69 1 69 6.57e-41 132
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780036822.01.T01 AT1G36240 86.957 69 9 0 1 69 1 69 2.03e-38 126
MsG0780036822.01.T01 AT3G18740 84.058 69 11 0 1 69 1 69 7.63e-37 122
MsG0780036822.01.T01 AT1G77940 82.609 69 12 0 1 69 1 69 3.26e-36 120

Find 38 sgRNAs with CRISPR-Local

Find 99 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAACTAAATTGATACTATT+GGG 0.264694 7:-14455860 None:intergenic
GTTCAGTGGATTAATGAGTA+TGG 0.286367 7:+14455878 MsG0780036822.01.T01:three_prime_UTR
CTGAACTAAATTGATACTAT+TGG 0.303827 7:-14455861 None:intergenic
ATGGTGTACGATGCGTATTT+TGG 0.316239 7:+14455685 MsG0780036822.01.T01:three_prime_UTR
CTATGCTATGTTGGCTAAGT+TGG 0.356019 7:+14454481 MsG0780036822.01.T01:CDS
CTAACAACTGTCCTCCTTTG+AGG 0.365472 7:+14454441 MsG0780036822.01.T01:CDS
GACATCATCAAGACAATTCC+TGG 0.382525 7:+14455573 MsG0780036822.01.T01:CDS
AAAATTCTAGTTGTAGACTA+TGG 0.397229 7:+14455666 MsG0780036822.01.T01:exon
ACAACTTACTTCCATTGTAA+TGG 0.400094 7:-14454510 None:intergenic
GGCCACCATTGTTGAATGTT+TGG 0.411363 7:-14453897 None:intergenic
TACTGCGTTTACTGATCTCC+AGG 0.439400 7:-14455591 None:intergenic
CTCATGAGAGCATCAACAAC+AGG 0.462326 7:+14454038 MsG0780036822.01.T01:CDS
TGCCTCAGCATTGTTGATCC+TGG 0.466033 7:+14455459 MsG0780036822.01.T01:CDS
GAAGCTTACGGTCTTCTTTG+CGG 0.470686 7:-14453918 None:intergenic
TTACTCAATGCTCAATCAGC+GGG 0.500250 7:-14455712 None:intergenic
AAGAGTGGAAAATATACCCT+TGG 0.505811 7:+14454076 MsG0780036822.01.T01:CDS
AAATCCCTCAGGAGTTCTAA+AGG 0.506686 7:+14454115 MsG0780036822.01.T01:CDS
ATAGTATCAATTTAGTTCAG+TGG 0.510268 7:+14455864 MsG0780036822.01.T01:three_prime_UTR
ACATAGAATTGAAAACATCA+TGG 0.511457 7:-14455623 None:intergenic
CTTGCTCTTGTTATGAAGAG+TGG 0.524886 7:+14454061 MsG0780036822.01.T01:CDS
GAACTAAATTGATACTATTG+GGG 0.534174 7:-14455859 None:intergenic
ATTACCTTTAGAACTCCTGA+GGG 0.545765 7:-14454119 None:intergenic
AATTGAGTACTATGCTATGT+TGG 0.552970 7:+14454472 MsG0780036822.01.T01:CDS
AGAGTGGAAAATATACCCTT+GGG 0.553555 7:+14454077 MsG0780036822.01.T01:CDS
AAACTGTTCTCAAATCCCTC+AGG 0.565600 7:+14454104 MsG0780036822.01.T01:CDS
GTTGACCTTGGCACTGCTTG+CGG 0.567329 7:+14455417 MsG0780036822.01.T01:CDS
ATCATAGACAATGTTGACCT+TGG 0.573586 7:+14455405 MsG0780036822.01.T01:intron
CATAGAATTGAAAACATCAT+GGG 0.585069 7:-14455622 None:intergenic
ATTTCAGACTTCCTCAAAGG+AGG 0.587118 7:-14454452 None:intergenic
TCAATTTCAGACTTCCTCAA+AGG 0.597003 7:-14454455 None:intergenic
GTTGGAGTTCACCATTACAA+TGG 0.600433 7:+14454499 MsG0780036822.01.T01:CDS
AATTACCTTTAGAACTCCTG+AGG 0.601529 7:-14454120 None:intergenic
AGGAATTGTCTTGATGATGT+CGG 0.611366 7:-14455571 None:intergenic
ACTTTCCGCAAGCAGTGCCA+AGG 0.618915 7:-14455422 None:intergenic
CACCAAACATTCAACAATGG+TGG 0.629371 7:+14453895 MsG0780036822.01.T01:exon
ATTACTCAATGCTCAATCAG+CGG 0.635785 7:-14455713 None:intergenic
TACCAGGATCAACAATGCTG+AGG 0.662178 7:-14455461 None:intergenic
TCACACCAAACATTCAACAA+TGG 0.690217 7:+14453892 MsG0780036822.01.T01:exon

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CAAATAACAAATAAAATTAG+AGG - Chr7:14455535-14455554 None:intergenic 15.0%
!!! CTCTAATTTTATTTGTTATT+TGG + Chr7:14455533-14455552 MsG0780036822.01.T01:intron 15.0%
!! TATAATTATCTATCAGTAGT+TGG + Chr7:14455093-14455112 MsG0780036822.01.T01:intron 20.0%
!! TGAACTAAATTGATACTATT+GGG - Chr7:14455863-14455882 None:intergenic 20.0%
!! TGACTTAACTTTATAAGATT+TGG + Chr7:14454991-14455010 MsG0780036822.01.T01:intron 20.0%
!!! AAGCTAGTTATACTTTAAAA+TGG + Chr7:14454733-14454752 MsG0780036822.01.T01:intron 20.0%
!!! TTGATTGAATTAAGTGATTT+TGG + Chr7:14454342-14454361 MsG0780036822.01.T01:intron 20.0%
!!! TTGTGTTGATTAATGATATA+GGG - Chr7:14454789-14454808 None:intergenic 20.0%
!!! TTTGATTTGATTATTCTGTA+GGG + Chr7:14454402-14454421 MsG0780036822.01.T01:intron 20.0%
!!! TTTGTGTTGATTAATGATAT+AGG - Chr7:14454790-14454809 None:intergenic 20.0%
!!! TTTTGATTTGATTATTCTGT+AGG + Chr7:14454401-14454420 MsG0780036822.01.T01:intron 20.0%
! AAATAAGTGATTAGTGAAAG+TGG + Chr7:14454296-14454315 MsG0780036822.01.T01:intron 25.0%
! AATTGTGTATGTATACCTTA+GGG + Chr7:14454828-14454847 MsG0780036822.01.T01:intron 25.0%
! ACATAGAATTGAAAACATCA+TGG - Chr7:14455626-14455645 None:intergenic 25.0%
! ATGATTTCGTTTCAGATTTA+GGG + Chr7:14453969-14453988 MsG0780036822.01.T01:intron 25.0%
! CATAGAATTGAAAACATCAT+GGG - Chr7:14455625-14455644 None:intergenic 25.0%
! CTGAACTAAATTGATACTAT+TGG - Chr7:14455864-14455883 None:intergenic 25.0%
! GAACTAAATTGATACTATTG+GGG - Chr7:14455862-14455881 None:intergenic 25.0%
! GTACTGAAAAATATTATACG+TGG + Chr7:14454937-14454956 MsG0780036822.01.T01:intron 25.0%
! TAAGAAGAATTCCAACAATT+TGG - Chr7:14455289-14455308 None:intergenic 25.0%
! TAATTGTGTATGTATACCTT+AGG + Chr7:14454827-14454846 MsG0780036822.01.T01:intron 25.0%
! TACACATTTCAACAACAAAA+TGG - Chr7:14454153-14454172 None:intergenic 25.0%
! TATGATTTCGTTTCAGATTT+AGG + Chr7:14453968-14453987 MsG0780036822.01.T01:intron 25.0%
! TCATGTTGAAGAATTATACA+TGG + Chr7:14454887-14454906 MsG0780036822.01.T01:intron 25.0%
! TTAGATTAGATTAGATTGCA+AGG + Chr7:14454211-14454230 MsG0780036822.01.T01:intron 25.0%
!! AAAATTCTAGTTGTAGACTA+TGG + Chr7:14455666-14455685 MsG0780036822.01.T01:exon 25.0%
!! ATACATAACAGAAGCATATT+TGG - Chr7:14455151-14455170 None:intergenic 25.0%
!! ATAGTATCAATTTAGTTCAG+TGG + Chr7:14455864-14455883 MsG0780036822.01.T01:three_prime_UTR 25.0%
!! CTACAACTAGAATTTTCTTT+CGG - Chr7:14455662-14455681 None:intergenic 25.0%
!! TTGTTGTTGAAATGTGTATT+GGG + Chr7:14454155-14454174 MsG0780036822.01.T01:intron 25.0%
!!! ATAATATTTTTCAGTACGAC+AGG - Chr7:14454934-14454953 None:intergenic 25.0%
!!! ATTTGTTATTTGGTGTTTTC+AGG + Chr7:14455543-14455562 MsG0780036822.01.T01:intron 25.0%
!!! TAATATTTTTCAGTACGACA+GGG - Chr7:14454933-14454952 None:intergenic 25.0%
!!! TTTGTTGTTGAAATGTGTAT+TGG + Chr7:14454154-14454173 MsG0780036822.01.T01:intron 25.0%
AATTGAGTACTATGCTATGT+TGG + Chr7:14454472-14454491 MsG0780036822.01.T01:CDS 30.0%
ACAACTTACTTCCATTGTAA+TGG - Chr7:14454513-14454532 None:intergenic 30.0%
TAGAATGATATGAAATGCAG+TGG + Chr7:14454694-14454713 MsG0780036822.01.T01:intron 30.0%
TTAATGACACAGAGAAGTAA+AGG - Chr7:14454547-14454566 None:intergenic 30.0%
TTCAATGTTGATATGCATCA+GGG + Chr7:14454570-14454589 MsG0780036822.01.T01:intron 30.0%
TTTCAATGTTGATATGCATC+AGG + Chr7:14454569-14454588 MsG0780036822.01.T01:intron 30.0%
! TGTTGATTAATGATATAGGG+TGG - Chr7:14454786-14454805 None:intergenic 30.0%
!! GTTGTAGATTGATTTTGTAG+TGG + Chr7:14454273-14454292 MsG0780036822.01.T01:intron 30.0%
!! TGAAATGCAGTGGATTAAAA+TGG + Chr7:14454704-14454723 MsG0780036822.01.T01:intron 30.0%
!! TTGTTGTTTCTCCAAATTGT+TGG + Chr7:14455275-14455294 MsG0780036822.01.T01:intron 30.0%
!! TTTGAATGTTGATTTGGTAG+TGG + Chr7:14454368-14454387 MsG0780036822.01.T01:intron 30.0%
!!! TGGAGTTTTGAATGTTGATT+TGG + Chr7:14454362-14454381 MsG0780036822.01.T01:intron 30.0%
AAGAGTGGAAAATATACCCT+TGG + Chr7:14454076-14454095 MsG0780036822.01.T01:CDS 35.0%
AATTATTACACGTGACCCTA+AGG - Chr7:14454846-14454865 None:intergenic 35.0%
AGAGTGGAAAATATACCCTT+GGG + Chr7:14454077-14454096 MsG0780036822.01.T01:CDS 35.0%
ATCATAGACAATGTTGACCT+TGG + Chr7:14455405-14455424 MsG0780036822.01.T01:intron 35.0%
ATTACTCAATGCTCAATCAG+CGG - Chr7:14455716-14455735 None:intergenic 35.0%
ATTTGATCTTACGCTGATGT+GGG + Chr7:14455029-14455048 MsG0780036822.01.T01:intron 35.0%
TATCTATCAGTAGTTGGATG+TGG + Chr7:14455099-14455118 MsG0780036822.01.T01:intron 35.0%
TCAATTTCAGACTTCCTCAA+AGG - Chr7:14454458-14454477 None:intergenic 35.0%
TCACACCAAACATTCAACAA+TGG + Chr7:14453892-14453911 MsG0780036822.01.T01:exon 35.0%
TGAAGAAAGCAACATATACC+AGG - Chr7:14455480-14455499 None:intergenic 35.0%
TGCTGAATGAAAGAAGCTTA+CGG - Chr7:14453933-14453952 None:intergenic 35.0%
! ATGGCTTAGTTTGATGATTG+TGG + Chr7:14455202-14455221 MsG0780036822.01.T01:intron 35.0%
! TACTGCATATGCTAGTTTGA+TGG + Chr7:14455183-14455202 MsG0780036822.01.T01:intron 35.0%
! TCTGTTATCAGTTGACTCTA+AGG + Chr7:14455329-14455348 MsG0780036822.01.T01:intron 35.0%
!! AATTACCTTTAGAACTCCTG+AGG - Chr7:14454123-14454142 None:intergenic 35.0%
!! AGGAATTGTCTTGATGATGT+CGG - Chr7:14455574-14455593 None:intergenic 35.0%
!! ATTACCTTTAGAACTCCTGA+GGG - Chr7:14454122-14454141 None:intergenic 35.0%
!! GAGAACAGTTTTATACCCAA+GGG - Chr7:14454095-14454114 None:intergenic 35.0%
!! GTTCAGTGGATTAATGAGTA+TGG + Chr7:14455878-14455897 MsG0780036822.01.T01:three_prime_UTR 35.0%
!! TGAGAACAGTTTTATACCCA+AGG - Chr7:14454096-14454115 None:intergenic 35.0%
AAACTGTTCTCAAATCCCTC+AGG + Chr7:14454104-14454123 MsG0780036822.01.T01:CDS 40.0%
ATGACACTACTGTACGATAC+AGG - Chr7:14454971-14454990 None:intergenic 40.0%
ATGCGCAGAGAAGAAAAATG+CGG - Chr7:14453871-14453890 None:intergenic 40.0%
ATTTCAGACTTCCTCAAAGG+AGG - Chr7:14454455-14454474 None:intergenic 40.0%
CACCAAACATTCAACAATGG+TGG + Chr7:14453895-14453914 MsG0780036822.01.T01:exon 40.0%
CACTGATACACACTAAAGCT+CGG - Chr7:14454671-14454690 None:intergenic 40.0%
GACATCATCAAGACAATTCC+TGG + Chr7:14455573-14455592 MsG0780036822.01.T01:CDS 40.0%
GATTTGATCTTACGCTGATG+TGG + Chr7:14455028-14455047 MsG0780036822.01.T01:intron 40.0%
GTTGGAGTTCACCATTACAA+TGG + Chr7:14454499-14454518 MsG0780036822.01.T01:CDS 40.0%
TATGCATCAGGGATTGTTTC+AGG + Chr7:14454581-14454600 MsG0780036822.01.T01:intron 40.0%
TCGGCTAGATAGAATCAATC+AGG - Chr7:14454652-14454671 None:intergenic 40.0%
TGACACTACTGTACGATACA+GGG - Chr7:14454970-14454989 None:intergenic 40.0%
TGATATAGGGTGGAATAGAG+TGG - Chr7:14454776-14454795 None:intergenic 40.0%
TTACTCAATGCTCAATCAGC+GGG - Chr7:14455715-14455734 None:intergenic 40.0%
! AAATCCCTCAGGAGTTCTAA+AGG + Chr7:14454115-14454134 MsG0780036822.01.T01:CDS 40.0%
! ATGGTGTACGATGCGTATTT+TGG + Chr7:14455685-14455704 MsG0780036822.01.T01:three_prime_UTR 40.0%
! CTATGCTATGTTGGCTAAGT+TGG + Chr7:14454481-14454500 MsG0780036822.01.T01:CDS 40.0%
! CTTGCTCTTGTTATGAAGAG+TGG + Chr7:14454061-14454080 MsG0780036822.01.T01:CDS 40.0%
! GATTGTGGATATGCTTTTGC+TGG + Chr7:14455217-14455236 MsG0780036822.01.T01:intron 40.0%
! GTTGCTTTCTTCAACTGTCT+TGG + Chr7:14455487-14455506 MsG0780036822.01.T01:intron 40.0%
! TGGTGTACGATGCGTATTTT+GGG + Chr7:14455686-14455705 MsG0780036822.01.T01:three_prime_UTR 40.0%
!! GAACAAAACAAGTGCTGATC+AGG - Chr7:14455247-14455266 None:intergenic 40.0%
CTAACAACTGTCCTCCTTTG+AGG + Chr7:14454441-14454460 MsG0780036822.01.T01:CDS 45.0%
GAAGCTTACGGTCTTCTTTG+CGG - Chr7:14453921-14453940 None:intergenic 45.0%
GGCCACCATTGTTGAATGTT+TGG - Chr7:14453900-14453919 None:intergenic 45.0%
TACCAGGATCAACAATGCTG+AGG - Chr7:14455464-14455483 None:intergenic 45.0%
TACTGCGTTTACTGATCTCC+AGG - Chr7:14455594-14455613 None:intergenic 45.0%
! CGAGCTTTAGTGTGTATCAG+TGG + Chr7:14454669-14454688 MsG0780036822.01.T01:intron 45.0%
!! CTCATGAGAGCATCAACAAC+AGG + Chr7:14454038-14454057 MsG0780036822.01.T01:CDS 45.0%
GAGAAGAAAAATGCGGCGAG+TGG - Chr7:14453864-14453883 None:intergenic 50.0%
TGCCTCAGCATTGTTGATCC+TGG + Chr7:14455459-14455478 MsG0780036822.01.T01:CDS 50.0%
ACTTTCCGCAAGCAGTGCCA+AGG - Chr7:14455425-14455444 None:intergenic 55.0%
GTTGACCTTGGCACTGCTTG+CGG + Chr7:14455417-14455436 MsG0780036822.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr7 gene 14453832 14455916 14453832 ID=MsG0780036822.01;Name=MsG0780036822.01
Chr7 mRNA 14453832 14455916 14453832 ID=MsG0780036822.01.T01;Parent=MsG0780036822.01;Name=MsG0780036822.01.T01;_AED=0.33;_eAED=0.33;_QI=79|1|1|1|0.75|0.6|5|241|135
Chr7 exon 14453832 14453931 14453832 ID=MsG0780036822.01.T01:exon:2285;Parent=MsG0780036822.01.T01
Chr7 exon 14454031 14454136 14454031 ID=MsG0780036822.01.T01:exon:2286;Parent=MsG0780036822.01.T01
Chr7 exon 14454423 14454523 14454423 ID=MsG0780036822.01.T01:exon:2287;Parent=MsG0780036822.01.T01
Chr7 exon 14455412 14455480 14455412 ID=MsG0780036822.01.T01:exon:2288;Parent=MsG0780036822.01.T01
Chr7 exon 14455565 14455916 14455565 ID=MsG0780036822.01.T01:exon:2289;Parent=MsG0780036822.01.T01
Chr7 five_prime_UTR 14453832 14453910 14453832 ID=MsG0780036822.01.T01:five_prime_utr;Parent=MsG0780036822.01.T01
Chr7 CDS 14453911 14453931 14453911 ID=MsG0780036822.01.T01:cds;Parent=MsG0780036822.01.T01
Chr7 CDS 14454031 14454136 14454031 ID=MsG0780036822.01.T01:cds;Parent=MsG0780036822.01.T01
Chr7 CDS 14454423 14454523 14454423 ID=MsG0780036822.01.T01:cds;Parent=MsG0780036822.01.T01
Chr7 CDS 14455412 14455480 14455412 ID=MsG0780036822.01.T01:cds;Parent=MsG0780036822.01.T01
Chr7 CDS 14455565 14455675 14455565 ID=MsG0780036822.01.T01:cds;Parent=MsG0780036822.01.T01
Chr7 three_prime_UTR 14455676 14455916 14455676 ID=MsG0780036822.01.T01:three_prime_utr;Parent=MsG0780036822.01.T01
Gene Sequence

>MsG0780036822.01.T01

ATGGTGGCCGCAAAGAAGACCAAGAAGACTCATGAGAGCATCAACAACAGGCTTGCTCTTGTTATGAAGAGTGGAAAATATACCCTTGGGTATAAAACTGTTCTCAAATCCCTCAGGAGTTCTAAAGGGAAATTGATTATCATTGCTAACAACTGTCCTCCTTTGAGGAAGTCTGAAATTGAGTACTATGCTATGTTGGCTAAGTTGGAGTTCACCATTACAATGGAAACAATGTTGACCTTGGCACTGCTTGCGGAAAGTACTACAGAGTGTGCTGCCTCAGCATTGTTGATCCTGGTGATTCCGACATCATCAAGACAATTCCTGGAGATCAGTAAACGCAGTAAAGATTTTCCCATGATGTTTTCAATTCTATGTTGTTTAGACATTTCCGAAAGAAAATTCTAG

Protein sequence

>MsG0780036822.01.T01

MVAAKKTKKTHESINNRLALVMKSGKYTLGYKTVLKSLRSSKGKLIIIANNCPPLRKSEIEYYAMLAKLEFTITMETMLTLALLAESTTECAASALLILVIPTSSRQFLEISKRSKDFPMMFSILCCLDISERKF*