Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024600.01.T01 | XP_003611409.1 | 98.347 | 121 | 2 | 0 | 1 | 121 | 423 | 543 | 7.05E-76 | 244 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024600.01.T01 | O23553 | 64.062 | 128 | 38 | 3 | 1 | 121 | 422 | 548 | 2.60E-45 | 157 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024600.01.T01 | G7JYN3 | 98.347 | 121 | 2 | 0 | 1 | 121 | 423 | 543 | 3.37e-76 | 244 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048849.01 | MsG0580024600.01 | 0.810555 | 1.087553e-50 | 6.575985e-48 |
| MsG0280011073.01 | MsG0580024600.01 | 0.821975 | 3.033759e-53 | 2.505787e-50 |
| MsG0380013467.01 | MsG0580024600.01 | 0.810627 | 1.049211e-50 | 6.356558e-48 |
| MsG0480020024.01 | MsG0580024600.01 | 0.801884 | 7.294590e-49 | 3.523350e-46 |
| MsG0480022239.01 | MsG0580024600.01 | 0.843837 | 1.096132e-58 | 1.736649e-55 |
| MsG0480022243.01 | MsG0580024600.01 | 0.800763 | 1.237472e-48 | 5.809488e-46 |
| MsG0480022245.01 | MsG0580024600.01 | 0.828039 | 1.121892e-54 | 1.102289e-51 |
| MsG0480023652.01 | MsG0580024600.01 | 0.828129 | 1.067273e-54 | 1.051348e-51 |
| MsG0580024597.01 | MsG0580024600.01 | 0.811943 | 5.437160e-51 | 3.410922e-48 |
| MsG0580024600.01 | MsG0580024601.01 | 0.802146 | 6.444086e-49 | 3.133221e-46 |
| MsG0580024600.01 | MsG0580029914.01 | 0.812607 | 3.893696e-51 | 2.486709e-48 |
| MsG0580024600.01 | MsG0680030555.01 | 0.814194 | 1.744137e-51 | 1.162575e-48 |
| MsG0580024600.01 | MsG0680030636.01 | 0.815304 | 9.903437e-52 | 6.802635e-49 |
| MsG0580024600.01 | MsG0680032516.01 | 0.840131 | 1.045712e-57 | 1.475559e-54 |
| MsG0580024600.01 | MsG0780035960.01 | 0.823583 | 1.281188e-53 | 1.107684e-50 |
| MsG0580024600.01 | MsG0780041729.01 | -0.815789 | 7.723197e-52 | 5.376494e-49 |
| MsG0580024600.01 | MsG0780041747.01 | 0.832606 | 8.587200e-56 | 9.646389e-53 |
| MsG0580024600.01 | MsG0880042086.01 | 0.805765 | 1.139619e-49 | 6.080393e-47 |
| MsG0480018426.01 | MsG0580024600.01 | 0.806914 | 6.526513e-50 | 3.585998e-47 |
| MsG0280008098.01 | MsG0580024600.01 | 0.817665 | 2.930535e-52 | 2.147538e-49 |
| MsG0280008928.01 | MsG0580024600.01 | 0.807559 | 4.765550e-50 | 2.662562e-47 |
| MsG0280008929.01 | MsG0580024600.01 | 0.823377 | 1.431461e-53 | 1.230284e-50 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024600.01.T01 | MTR_5g013640 | 98.347 | 121 | 2 | 0 | 1 | 121 | 423 | 543 | 8.54e-80 | 244 |
| MsG0580024600.01.T01 | MTR_5g013620 | 90.000 | 120 | 10 | 1 | 1 | 120 | 425 | 542 | 3.62e-71 | 222 |
| MsG0580024600.01.T01 | MTR_4g099110 | 67.500 | 120 | 32 | 2 | 1 | 113 | 414 | 533 | 1.26e-47 | 160 |
| MsG0580024600.01.T01 | MTR_6g477780 | 50.515 | 97 | 37 | 1 | 1 | 86 | 255 | 351 | 7.98e-25 | 97.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024600.01.T01 | AT4G17090 | 64.062 | 128 | 38 | 3 | 1 | 121 | 422 | 548 | 2.65e-46 | 157 |
| MsG0580024600.01.T01 | AT3G23920 | 48.485 | 99 | 38 | 1 | 1 | 86 | 443 | 541 | 1.91e-22 | 91.7 |
| MsG0580024600.01.T01 | AT2G32290 | 36.170 | 94 | 49 | 1 | 1 | 83 | 413 | 506 | 2.60e-11 | 59.7 |
Find 26 sgRNAs with CRISPR-Local
Find 28 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTTATGTTGGACACATTAA+AGG | 0.225336 | 5:-6778176 | MsG0580024600.01.T01:CDS |
| TGGACACATTAAAGGAATTA+AGG | 0.290030 | 5:-6778168 | MsG0580024600.01.T01:CDS |
| GGTTGCTTGAGGGTGAAAAT+TGG | 0.313491 | 5:-6778280 | MsG0580024600.01.T01:CDS |
| CTTTCAAAATCTGATTCATA+TGG | 0.321608 | 5:-6778206 | MsG0580024600.01.T01:CDS |
| AGATATGATTCAAGTGCTTA+TGG | 0.392336 | 5:-6778359 | MsG0580024600.01.T01:CDS |
| TGAAAATTGGAGGCAATTTG+TGG | 0.443560 | 5:-6778267 | MsG0580024600.01.T01:CDS |
| TATGGGACTGATCTTTATGT+TGG | 0.456464 | 5:-6778188 | MsG0580024600.01.T01:CDS |
| AAGATGGCAACAAAGATTGC+AGG | 0.463188 | 5:-6778413 | MsG0580024600.01.T01:CDS |
| ACTTGATTCACCAATCCTTC+CGG | 0.472396 | 5:+6778437 | None:intergenic |
| TTTCAAAATCTGATTCATAT+GGG | 0.479314 | 5:-6778205 | MsG0580024600.01.T01:CDS |
| ATTGCAGGTGGTGAACTTGC+TGG | 0.483856 | 5:-6778398 | MsG0580024600.01.T01:CDS |
| TAATTGTTCACCGGAAGGAT+TGG | 0.517053 | 5:-6778447 | MsG0580024600.01.T01:CDS |
| ATTGGTGAATCAAGTGAAGA+TGG | 0.538317 | 5:-6778429 | MsG0580024600.01.T01:CDS |
| ATAAACAAGAGGTTGCTTGA+GGG | 0.540389 | 5:-6778290 | MsG0580024600.01.T01:CDS |
| TGCTTGAGGGTGAAAATTGG+AGG | 0.540642 | 5:-6778277 | MsG0580024600.01.T01:CDS |
| CTTATTTAAGAATAAACAAG+AGG | 0.556225 | 5:-6778301 | MsG0580024600.01.T01:CDS |
| CATGCTAATTGTTCACCGGA+AGG | 0.556485 | 5:-6778452 | MsG0580024600.01.T01:CDS |
| TTGCCATGCTAATTGTTCAC+CGG | 0.569138 | 5:-6778456 | MsG0580024600.01.T01:CDS |
| AATAAACAAGAGGTTGCTTG+AGG | 0.573981 | 5:-6778291 | MsG0580024600.01.T01:CDS |
| GGTGAACAATTAGCATGGCA+AGG | 0.606783 | 5:+6778458 | None:intergenic |
| TTTGTTGTGAGTATGTCTGA+TGG | 0.609724 | 5:-6778242 | MsG0580024600.01.T01:CDS |
| ACTTGCTGGTGAGAATGCAT+TGG | 0.612219 | 5:-6778384 | MsG0580024600.01.T01:CDS |
| GTTGTGAGTATGTCTGATGG+TGG | 0.636973 | 5:-6778239 | MsG0580024600.01.T01:CDS |
| CTTCCGGTGAACAATTAGCA+TGG | 0.643708 | 5:+6778453 | None:intergenic |
| ATGGCAACAAAGATTGCAGG+TGG | 0.675921 | 5:-6778410 | MsG0580024600.01.T01:CDS |
| TGTCTGATGGTGGTAAACCA+AGG | 0.696376 | 5:-6778229 | MsG0580024600.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | CTTATTTAAGAATAAACAAG+AGG | - | Chr5:6778302-6778321 | MsG0580024600.01.T01:CDS | 20.0% |
| !! | TTTCAAAATCTGATTCATAT+GGG | - | Chr5:6778398-6778417 | MsG0580024600.01.T01:CDS | 20.0% |
| ! | CTTTCAAAATCTGATTCATA+TGG | - | Chr5:6778397-6778416 | MsG0580024600.01.T01:CDS | 25.0% |
| AGATATGATTCAAGTGCTTA+TGG | - | Chr5:6778244-6778263 | MsG0580024600.01.T01:CDS | 30.0% | |
| CTTTATGTTGGACACATTAA+AGG | - | Chr5:6778427-6778446 | MsG0580024600.01.T01:CDS | 30.0% | |
| TGGACACATTAAAGGAATTA+AGG | - | Chr5:6778435-6778454 | MsG0580024600.01.T01:CDS | 30.0% | |
| AATAAACAAGAGGTTGCTTG+AGG | - | Chr5:6778312-6778331 | MsG0580024600.01.T01:CDS | 35.0% | |
| ATAAACAAGAGGTTGCTTGA+GGG | - | Chr5:6778313-6778332 | MsG0580024600.01.T01:CDS | 35.0% | |
| ATTGGTGAATCAAGTGAAGA+TGG | - | Chr5:6778174-6778193 | MsG0580024600.01.T01:CDS | 35.0% | |
| TATGGGACTGATCTTTATGT+TGG | - | Chr5:6778415-6778434 | MsG0580024600.01.T01:CDS | 35.0% | |
| TGAAAATTGGAGGCAATTTG+TGG | - | Chr5:6778336-6778355 | MsG0580024600.01.T01:CDS | 35.0% | |
| ! | TTTGTTGTGAGTATGTCTGA+TGG | - | Chr5:6778361-6778380 | MsG0580024600.01.T01:CDS | 35.0% |
| !! | GAATCAGATTTTGAAAGCCT+TGG | + | Chr5:6778394-6778413 | None:intergenic | 35.0% |
| AAGATGGCAACAAAGATTGC+AGG | - | Chr5:6778190-6778209 | MsG0580024600.01.T01:CDS | 40.0% | |
| ACTTGATTCACCAATCCTTC+CGG | + | Chr5:6778169-6778188 | None:intergenic | 40.0% | |
| TAATTGTTCACCGGAAGGAT+TGG | - | Chr5:6778156-6778175 | MsG0580024600.01.T01:CDS | 40.0% | |
| TTGCCATGCTAATTGTTCAC+CGG | - | Chr5:6778147-6778166 | MsG0580024600.01.T01:CDS | 40.0% | |
| !! | GGTCAAGTTTTGTCAACAAG+TGG | - | Chr5:6778265-6778284 | MsG0580024600.01.T01:CDS | 40.0% |
| ATGGCAACAAAGATTGCAGG+TGG | - | Chr5:6778193-6778212 | MsG0580024600.01.T01:CDS | 45.0% | |
| CATGCTAATTGTTCACCGGA+AGG | - | Chr5:6778151-6778170 | MsG0580024600.01.T01:CDS | 45.0% | |
| CTTCCGGTGAACAATTAGCA+TGG | + | Chr5:6778153-6778172 | None:intergenic | 45.0% | |
| GGTGAACAATTAGCATGGCA+AGG | + | Chr5:6778148-6778167 | None:intergenic | 45.0% | |
| GGTTGCTTGAGGGTGAAAAT+TGG | - | Chr5:6778323-6778342 | MsG0580024600.01.T01:CDS | 45.0% | |
| GTTGTGAGTATGTCTGATGG+TGG | - | Chr5:6778364-6778383 | MsG0580024600.01.T01:CDS | 45.0% | |
| TGCTTGAGGGTGAAAATTGG+AGG | - | Chr5:6778326-6778345 | MsG0580024600.01.T01:CDS | 45.0% | |
| ! | ACTTGCTGGTGAGAATGCAT+TGG | - | Chr5:6778219-6778238 | MsG0580024600.01.T01:CDS | 45.0% |
| !! | TGTCTGATGGTGGTAAACCA+AGG | - | Chr5:6778374-6778393 | MsG0580024600.01.T01:CDS | 45.0% |
| ATTGCAGGTGGTGAACTTGC+TGG | - | Chr5:6778205-6778224 | MsG0580024600.01.T01:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 6778127 | 6778498 | 6778127 | ID=MsG0580024600.01;Name=MsG0580024600.01 |
| Chr5 | mRNA | 6778127 | 6778498 | 6778127 | ID=MsG0580024600.01.T01;Parent=MsG0580024600.01;Name=MsG0580024600.01.T01;_AED=0.41;_eAED=0.41;_QI=0|-1|0|1|-1|1|1|0|123 |
| Chr5 | exon | 6778127 | 6778498 | 6778127 | ID=MsG0580024600.01.T01:exon:29201;Parent=MsG0580024600.01.T01 |
| Chr5 | CDS | 6778127 | 6778498 | 6778127 | ID=MsG0580024600.01.T01:cds;Parent=MsG0580024600.01.T01 |
| Gene Sequence |
| Protein sequence |
