Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024835.01.T01 | XP_003611762.1 | 94.118 | 119 | 6 | 1 | 1 | 118 | 39 | 157 | 4.77E-75 | 230 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024835.01.T01 | G7K7Z5 | 94.118 | 119 | 6 | 1 | 1 | 118 | 39 | 157 | 2.28e-75 | 230 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180004471.01 | MsG0580024835.01 | 0.824220 | 9.084828e-54 | 7.996783e-51 |
| MsG0180004661.01 | MsG0580024835.01 | 0.801667 | 8.080410e-49 | 3.881261e-46 |
| MsG0180005048.01 | MsG0580024835.01 | 0.807328 | 5.332566e-50 | 2.961756e-47 |
| MsG0180006133.01 | MsG0580024835.01 | 0.804511 | 2.085290e-49 | 1.077172e-46 |
| MsG0280010798.01 | MsG0580024835.01 | 0.802118 | 6.528571e-49 | 3.171976e-46 |
| MsG0380012818.01 | MsG0580024835.01 | 0.807783 | 4.270439e-50 | 2.399994e-47 |
| MsG0480022784.01 | MsG0580024835.01 | 0.803340 | 3.652164e-49 | 1.830629e-46 |
| MsG0480023653.01 | MsG0580024835.01 | 0.824705 | 6.982085e-54 | 6.233102e-51 |
| MsG0480023706.01 | MsG0580024835.01 | 0.827831 | 1.258700e-54 | 1.229065e-51 |
| MsG0580024835.01 | MsG0680035792.01 | 0.805718 | 1.165766e-49 | 6.212487e-47 |
| MsG0580024835.01 | MsG0780040641.01 | 0.807044 | 6.126214e-50 | 3.377413e-47 |
| MsG0580024835.01 | MsG0780041660.01 | 0.821003 | 5.085806e-53 | 4.088367e-50 |
| MsG0580024835.01 | MsG0880047059.01 | 0.800019 | 1.753598e-48 | 8.078832e-46 |
| MsG0380016091.01 | MsG0580024835.01 | 0.801542 | 8.571066e-49 | 4.104017e-46 |
| MsG0380017181.01 | MsG0580024835.01 | 0.804824 | 1.794800e-49 | 9.347221e-47 |
| MsG0380017282.01 | MsG0580024835.01 | 0.818115 | 2.319359e-52 | 1.720735e-49 |
| MsG0380017588.01 | MsG0580024835.01 | 0.809600 | 1.746149e-50 | 1.029715e-47 |
| MsG0480018106.01 | MsG0580024835.01 | 0.809574 | 1.769301e-50 | 1.042601e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024835.01.T01 | MTR_5g017580 | 94.118 | 119 | 6 | 1 | 1 | 118 | 39 | 157 | 5.78e-79 | 230 |
| MsG0580024835.01.T01 | MTR_4g086340 | 40.541 | 111 | 57 | 3 | 15 | 123 | 29 | 132 | 3.29e-16 | 70.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024835.01.T01 | AT5G66800 | 46.939 | 98 | 35 | 3 | 16 | 96 | 54 | 151 | 9.83e-22 | 85.5 |
| MsG0580024835.01.T01 | AT3G50640 | 40.594 | 101 | 47 | 3 | 7 | 96 | 40 | 138 | 1.41e-15 | 68.9 |
Find 20 sgRNAs with CRISPR-Local
Find 25 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTTCTTTCTTGTTGTTTCA+TGG | 0.185452 | 5:-10471943 | None:intergenic |
| GGGTAGGTGGAAGGGTCTTT+TGG | 0.224869 | 5:+10472169 | MsG0580024835.01.T01:CDS |
| TTCACTCTTCTCAGCCTTCT+TGG | 0.245592 | 5:-10472216 | None:intergenic |
| TTAAATTGAGGTGGTTCATT+AGG | 0.253017 | 5:-10472257 | None:intergenic |
| TAAATTGAGGTGGTTCATTA+GGG | 0.360337 | 5:-10472256 | None:intergenic |
| TCAATTTAATTTCACTTCTC+AGG | 0.373437 | 5:+10472271 | MsG0580024835.01.T01:CDS |
| TGCATCTTATTATCTTTGTA+AGG | 0.373486 | 5:-10472068 | None:intergenic |
| GTAAGGCAACAATCTACCTT+TGG | 0.420353 | 5:-10472051 | None:intergenic |
| CTTAGGAGGCCTTAAAGAAA+AGG | 0.457709 | 5:-10472147 | None:intergenic |
| AGACCCTTCCACCTACCCTT+AGG | 0.492979 | 5:-10472164 | None:intergenic |
| GCCTCCTAAGGGTAGGTGGA+AGG | 0.507774 | 5:+10472160 | MsG0580024835.01.T01:CDS |
| CTTTAAGGCCTCCTAAGGGT+AGG | 0.520677 | 5:+10472153 | MsG0580024835.01.T01:CDS |
| CCTCCTAAGGGTAGGTGGAA+GGG | 0.523056 | 5:+10472161 | MsG0580024835.01.T01:CDS |
| TCAAAGTCAGAAGACACAAC+AGG | 0.536157 | 5:-10471981 | None:intergenic |
| CCCTTCCACCTACCCTTAGG+AGG | 0.580007 | 5:-10472161 | None:intergenic |
| TGAGAAGTGAAATTAAATTG+AGG | 0.588827 | 5:-10472269 | None:intergenic |
| AAGTCACATTGGTTCCAAGA+AGG | 0.590892 | 5:+10472202 | MsG0580024835.01.T01:CDS |
| GAAGTGAAATTAAATTGAGG+TGG | 0.603907 | 5:-10472266 | None:intergenic |
| TAAGGCCTCCTAAGGGTAGG+TGG | 0.616107 | 5:+10472156 | MsG0580024835.01.T01:CDS |
| AAGAAGGCTGAGAAGAGTGA+AGG | 0.616835 | 5:+10472218 | MsG0580024835.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATATGATTCCTTTTCTTTA+AGG | + | Chr5:10472138-10472157 | MsG0580024835.01.T01:CDS | 20.0% |
| ! | TGAGAAGTGAAATTAAATTG+AGG | - | Chr5:10472272-10472291 | None:intergenic | 25.0% |
| ! | TGCATCTTATTATCTTTGTA+AGG | - | Chr5:10472071-10472090 | None:intergenic | 25.0% |
| !! | TCAATTTAATTTCACTTCTC+AGG | + | Chr5:10472271-10472290 | MsG0580024835.01.T01:CDS | 25.0% |
| GAAGTGAAATTAAATTGAGG+TGG | - | Chr5:10472269-10472288 | None:intergenic | 30.0% | |
| TAAATTGAGGTGGTTCATTA+GGG | - | Chr5:10472259-10472278 | None:intergenic | 30.0% | |
| TTAAATTGAGGTGGTTCATT+AGG | - | Chr5:10472260-10472279 | None:intergenic | 30.0% | |
| GGTCTTAGAAAAAGTCACAT+TGG | + | Chr5:10472191-10472210 | MsG0580024835.01.T01:CDS | 35.0% | |
| !!! | TTTTCTTTAAGGCCTCCTAA+GGG | + | Chr5:10472149-10472168 | MsG0580024835.01.T01:CDS | 35.0% |
| CTTAGGAGGCCTTAAAGAAA+AGG | - | Chr5:10472150-10472169 | None:intergenic | 40.0% | |
| GTAAGGCAACAATCTACCTT+TGG | - | Chr5:10472054-10472073 | None:intergenic | 40.0% | |
| TCAAAGTCAGAAGACACAAC+AGG | - | Chr5:10471984-10472003 | None:intergenic | 40.0% | |
| ! | GCTGATGAACTTTTCTCCAA+AGG | + | Chr5:10472035-10472054 | MsG0580024835.01.T01:CDS | 40.0% |
| !! | AAGTCACATTGGTTCCAAGA+AGG | + | Chr5:10472202-10472221 | MsG0580024835.01.T01:CDS | 40.0% |
| !!! | CTTTTCTTTAAGGCCTCCTA+AGG | + | Chr5:10472148-10472167 | MsG0580024835.01.T01:CDS | 40.0% |
| AAGAAGGCTGAGAAGAGTGA+AGG | + | Chr5:10472218-10472237 | MsG0580024835.01.T01:CDS | 45.0% | |
| TTCACTCTTCTCAGCCTTCT+TGG | - | Chr5:10472219-10472238 | None:intergenic | 45.0% | |
| ! | GGTAGGTGGAAGGGTCTTTT+GGG | + | Chr5:10472170-10472189 | MsG0580024835.01.T01:CDS | 50.0% |
| !! | CTTTAAGGCCTCCTAAGGGT+AGG | + | Chr5:10472153-10472172 | MsG0580024835.01.T01:CDS | 50.0% |
| AGACCCTTCCACCTACCCTT+AGG | - | Chr5:10472167-10472186 | None:intergenic | 55.0% | |
| CCTCCTAAGGGTAGGTGGAA+GGG | + | Chr5:10472161-10472180 | MsG0580024835.01.T01:CDS | 55.0% | |
| TAAGGCCTCCTAAGGGTAGG+TGG | + | Chr5:10472156-10472175 | MsG0580024835.01.T01:CDS | 55.0% | |
| ! | GGGTAGGTGGAAGGGTCTTT+TGG | + | Chr5:10472169-10472188 | MsG0580024835.01.T01:CDS | 55.0% |
| CCCTTCCACCTACCCTTAGG+AGG | - | Chr5:10472164-10472183 | None:intergenic | 60.0% | |
| GCCTCCTAAGGGTAGGTGGA+AGG | + | Chr5:10472160-10472179 | MsG0580024835.01.T01:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 10471945 | 10472316 | 10471945 | ID=MsG0580024835.01;Name=MsG0580024835.01 |
| Chr5 | mRNA | 10471945 | 10472316 | 10471945 | ID=MsG0580024835.01.T01;Parent=MsG0580024835.01;Name=MsG0580024835.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|123 |
| Chr5 | exon | 10471945 | 10472316 | 10471945 | ID=MsG0580024835.01.T01:exon:1959;Parent=MsG0580024835.01.T01 |
| Chr5 | CDS | 10471945 | 10472316 | 10471945 | ID=MsG0580024835.01.T01:cds;Parent=MsG0580024835.01.T01 |
| Gene Sequence |
| Protein sequence |
