Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580029507.01.T01 | XP_003617076.1 | 96.701 | 576 | 18 | 1 | 1 | 576 | 1 | 575 | 0 | 1144 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000483.01 | MsG0580029507.01 | 0.823089 | 1.670910e-53 | 1.424693e-50 |
| MsG0180000854.01 | MsG0580029507.01 | 0.834594 | 2.738192e-56 | 3.264681e-53 |
| MsG0180002796.01 | MsG0580029507.01 | 0.813131 | 2.990026e-51 | 1.936220e-48 |
| MsG0180006103.01 | MsG0580029507.01 | 0.821411 | 4.095575e-53 | 3.329452e-50 |
| MsG0180006104.01 | MsG0580029507.01 | 0.853343 | 2.545156e-61 | 5.481809e-58 |
| MsG0280007756.01 | MsG0580029507.01 | 0.801151 | 1.030682e-48 | 4.886874e-46 |
| MsG0280009117.01 | MsG0580029507.01 | 0.806984 | 6.306146e-50 | 3.471222e-47 |
| MsG0280009157.01 | MsG0580029507.01 | 0.844769 | 6.155921e-59 | 1.004275e-55 |
| MsG0380013471.01 | MsG0580029507.01 | 0.826177 | 3.129542e-54 | 2.913261e-51 |
| MsG0380014214.01 | MsG0580029507.01 | 0.829870 | 4.041872e-55 | 4.189768e-52 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580029507.01.T01 | MTR_5g087680 | 96.701 | 576 | 18 | 1 | 1 | 576 | 1 | 575 | 0.0 | 1144 |
| MsG0580029507.01.T01 | MTR_8g028700 | 29.126 | 206 | 126 | 8 | 204 | 400 | 278 | 472 | 1.32e-13 | 73.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580029507.01.T01 | AT5G21040 | 65.708 | 487 | 149 | 4 | 94 | 576 | 67 | 539 | 0.0 | 639 |
| MsG0580029507.01.T01 | AT5G21040 | 65.708 | 487 | 149 | 4 | 94 | 576 | 67 | 539 | 0.0 | 639 |
| MsG0580029507.01.T01 | AT5G21040 | 65.708 | 487 | 149 | 4 | 94 | 576 | 67 | 539 | 0.0 | 639 |
| MsG0580029507.01.T01 | AT4G25440 | 26.562 | 192 | 124 | 6 | 215 | 400 | 201 | 381 | 7.08e-11 | 65.1 |
| MsG0580029507.01.T01 | AT4G25440 | 26.562 | 192 | 124 | 6 | 215 | 400 | 201 | 381 | 7.08e-11 | 65.1 |
| MsG0580029507.01.T01 | AT4G25440 | 26.562 | 192 | 124 | 6 | 215 | 400 | 201 | 381 | 7.08e-11 | 65.1 |
Find 167 sgRNAs with CRISPR-Local
Find 188 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACCCCAGAATCCGAATCCG+GGG | 0.606591 | 5:-97867912 | None:intergenic |
| AAGAAGGTGTTGTCAGGATT+TGG | 0.289321 | 5:+97868959 | MsG0580029507.01.T01:CDS |
| AAGCATGATGCTCAGAAGCA+AGG | 0.599509 | 5:-97867838 | None:intergenic |
| AAGCTCTGCATGTTGCTACA+TGG | 0.480913 | 5:+97868680 | MsG0580029507.01.T01:CDS |
| AAGGATTTATAGCGGCTCTT+GGG | 0.490823 | 5:+97868366 | MsG0580029507.01.T01:CDS |
| AATATCGAGCTTCGACTTTG+GGG | 0.580619 | 5:+97868304 | MsG0580029507.01.T01:CDS |
| AATCCATGCAACATCCTCTG+TGG | 0.681299 | 5:-97868880 | None:intergenic |
| AATTCTATTGTGAAAGGTGG+GGG | 0.539393 | 5:+97867867 | MsG0580029507.01.T01:CDS |
| AATTGGCACTAATTGACGTG+AGG | 0.673669 | 5:+97868776 | MsG0580029507.01.T01:CDS |
| ACAAGTACCAAATCTGAATC+TGG | 0.429740 | 5:+97867593 | MsG0580029507.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AGGTTCAAATGTCTATGTTT+GGG | + | Chr5:97868501-97868520 | MsG0580029507.01.T01:CDS | 30.0% | |
| CAAAATTTGGTAGAAAATCC+AGG | + | Chr5:97867494-97867513 | MsG0580029507.01.T01:CDS | 30.0% | |
| GATTTGACAAGGATTTATAG+CGG | + | Chr5:97868358-97868377 | MsG0580029507.01.T01:CDS | 30.0% | |
| GGATACAAATAGTGGTAATT+TGG | + | Chr5:97868522-97868541 | MsG0580029507.01.T01:CDS | 30.0% | |
| TAAAAATGAAATCCCCAGTA+TGG | - | Chr5:97868600-97868619 | None:intergenic | 30.0% | |
| TATAGTATGGATGTGTTGTA+TGG | + | Chr5:97868016-97868035 | MsG0580029507.01.T01:CDS | 30.0% | |
| TGGTACTTGTAACAAAATCA+GGG | - | Chr5:97867583-97867602 | None:intergenic | 30.0% | |
| TTGGTACTTGTAACAAAATC+AGG | - | Chr5:97867584-97867603 | None:intergenic | 30.0% | |
| AATTCTATTGTGAAAGGTGG+GGG | + | Chr5:97867867-97867886 | MsG0580029507.01.T01:CDS | 35.0% | |
| ACAAGTACCAAATCTGAATC+TGG | + | Chr5:97867593-97867612 | MsG0580029507.01.T01:CDS | 35.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 97867458 | 97869188 | 97867458 | ID=MsG0580029507.01;Name=MsG0580029507.01 |
| Chr5 | mRNA | 97867458 | 97869188 | 97867458 | ID=MsG0580029507.01.T01;Parent=MsG0580029507.01;Name=MsG0580029507.01.T01;_AED=0.40;_eAED=0.40;_QI=0|-1|0|1|-1|1|1|0|576 |
| Chr5 | exon | 97867458 | 97869188 | 97867458 | ID=MsG0580029507.01.T01:exon:25315;Parent=MsG0580029507.01.T01 |
| Chr5 | CDS | 97867458 | 97869188 | 97867458 | ID=MsG0580029507.01.T01:cds;Parent=MsG0580029507.01.T01 |
| Gene Sequence |
>MsG0580029507.01.T01 ATGGCATTTGATTGTCCACAGAGTATTAAGGTTTCTCAAAATTTGGTAGAAAATCCAGGTATAAGCATAGACATAGTTGAATTGAATCCAAAAGCTAATTCCAAAGCAATTTCAATTTCATTCCCTGATTTTGTTACAAGTACCAAATCTGAATCTGGTAAAGGTGAGATTTTTAAGGGGAAATCCAAGCTGAATTCTAAGAAAGGAATCAAAGCAGCCTCTGCTGGTGCTTTGAATCAATCATCAGTTCCTGGTGATGTTGTTATTGGTAATTGTCGGTCAATTACCGACCTGCCTCCGGTGTTGATATCTGAGATTCTGAATTGTCTTGATCCCAAGGAACTTGGAATTGTTTCATGTGTGTCGTCGATTCTGCGTAGCCTTGCTTCTGAGCATCATGCTTGGAAGGAATTCTATTGTGAAAGGTGGGGGCTTCCAGTAGTTCCTGAGGCTGCCCCGGATTCGGATTCTGGGGTTGGGGACTCGGTTAAGGATGAAAAGTCATGGAAGGATATTTTTGTGGAAAGGGATTATAGGAGTAAGACTTTTATGGGTAGGTATAGTATGGATGTGTTGTATGGCCATACTGAGGCGGTTCGGACGGTTTTCTTGTTGGCTTCTGCAAGGCTTATATTTACCTCTGGGTATGACACTGTTGTGAGGATGTGGAATATGGAAAGTGGGTTGTCGGTTGCATCGTCTAAACCCCTTGGCTGCACCATTCGTGCGGTTGCAGCTGATTCAAGGCTGCTAGTTGCTGGTGGTACTGATGGGTTCATTCATTGTTGGAGGGCTGTTGAAGGTTTGCCCCATCTGTTTGAGCTTAGAAGCTCACAACAAAACAAGAATATCGAGCTTCGACTTTGGGGTCATGATGGTCCGGTTACTTCACTTGCTTTAGATTTGACAAGGATTTATAGCGGCTCTTGGGACACGACTGTTCGTGTTTGGGACCGGCACTCAATGAAATGCACTGTGGTGTTGAGGCATAGTGACTGGGTTTGGGGACTTGTCCCTCATGATACTACAGTTGTCAGCGCATCAGGTTCAAATGTCTATGTTTGGGATACAAATAGTGGTAATTTGGTGACTGTTGTTCTAAATGCTCATGTTGGTAATACTTATGCTCTGGCAAGAAGCCATACTGGGGATTTCATTTTTACTGGGGGTGAAGATGGTTCAATTCACATGTACGAGATTGTTGACGATAGTTATGTGACCGAAGCTCTGCATGTTGCTACATGGGATCCCCACTCTGGTCCTGTGTATTCCCTTGCATTTGAGTTTCCTTGGCTTGTTTCTGCATCAAGTGACGGTAAATTGGCACTAATTGACGTGAGGAAGCTGCTGCGCAGGAGCAAGCGTGCCATTGGAAAGAGAGCTACGAAGGCAAAGCATTCGGGCGAAGTTAACGTAGAACCTCCACAGAGGATGTTGCATGGATTTAAGAATAATCTTTTCTCTGTAGGTATAGGAGCTGATCGAATTGTTTGCGGAGGTGAAGAAGGTGTTGTCAGGATTTGGAATTTCACTGAAGCTTTGGAAATTGAAAGCAGAGTCCGTGCGTTGAGAGGAATGCGATTAGAGAACAGAATGAGACGACGTAAGAACCAAACAGAGCTCAACAGTAAAGGCGGTAAGAGTGATCAATGTTCAGCTGTGGCCAAGAAGAATTCAGTGACTTGTATTTGGCCGAGTAAACGTGGGATGGGTGGAAAGACAAAGGCATAG |
| Protein sequence |
>MsG0580029507.01.T01 MAFDCPQSIKVSQNLVENPGISIDIVELNPKANSKAISISFPDFVTSTKSESGKGEIFKGKSKLNSKKGIKAASAGALNQSSVPGDVVIGNCRSITDLPPVLISEILNCLDPKELGIVSCVSSILRSLASEHHAWKEFYCERWGLPVVPEAAPDSDSGVGDSVKDEKSWKDIFVERDYRSKTFMGRYSMDVLYGHTEAVRTVFLLASARLIFTSGYDTVVRMWNMESGLSVASSKPLGCTIRAVAADSRLLVAGGTDGFIHCWRAVEGLPHLFELRSSQQNKNIELRLWGHDGPVTSLALDLTRIYSGSWDTTVRVWDRHSMKCTVVLRHSDWVWGLVPHDTTVVSASGSNVYVWDTNSGNLVTVVLNAHVGNTYALARSHTGDFIFTGGEDGSIHMYEIVDDSYVTEALHVATWDPHSGPVYSLAFEFPWLVSASSDGKLALIDVRKLLRRSKRAIGKRATKAKHSGEVNVEPPQRMLHGFKNNLFSVGIGADRIVCGGEEGVVRIWNFTEALEIESRVRALRGMRLENRMRRRKNQTELNSKGGKSDQCSAVAKKNSVTCIWPSKRGMGGKTKA* |
