AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580024650.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580024650.01.T01 MTR_4g117260 87.179 78 9 1 124 200 1 78 1.93e-40 135
MsG0580024650.01.T01 MTR_8g083120 83.333 78 12 1 124 200 1 78 2.40e-39 132
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580024650.01.T01 AT4G21110 73.077 78 20 1 124 200 1 78 1.81e-35 122
MsG0580024650.01.T01 AT4G21110 73.077 78 20 1 124 200 1 78 1.81e-35 122

Find 54 sgRNAs with CRISPR-Local

Find 210 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAGCTTTCTCCTGCGGATTT+TGG 0.079399 5:-7726537 MsG0580024650.01.T01:CDS
TCAATTAATTCCCAGCCTTC+TGG 0.227144 5:+7724533 None:intergenic
TAGAAATTTCCTTTCTCTTA+TGG 0.231250 5:+7724278 None:intergenic
TGGGCACTAGCCCGCCTAGC+TGG 0.233751 5:+7726673 None:intergenic
AAATCCTAGTTTACCTCATC+TGG 0.262161 5:+7726439 None:intergenic
ACTCGGCTTGTCTTTACTTT+TGG 0.267712 5:+7724563 None:intergenic
CAAGGAACAAGTCTAGTAAA+TGG 0.281166 5:-7726771 MsG0580024650.01.T01:CDS
TTGTTCTCTTTAATATCATT+TGG 0.315998 5:-7726721 MsG0580024650.01.T01:CDS
CAAATACATAGCGGCTCTTC+TGG 0.318337 5:+7724308 None:intergenic
GGGCACTAGCCCGCCTAGCT+GGG 0.324815 5:+7726674 None:intergenic
CCTAAATCCTTGGCAAAATC+TGG 0.346527 5:+7726465 None:intergenic
CAATTAATTCCCAGCCTTCT+GGG 0.361501 5:+7724534 None:intergenic
AGCTAGGCGGGCTAGTGCCC+AGG 0.401671 5:-7726671 MsG0580024650.01.T01:CDS
CTCAAAGTTGTAGTAATGGC+TGG 0.404762 5:+7726619 None:intergenic
TAAGGTGAATGAGTGTCGTT+TGG 0.406089 5:-7726801 None:intergenic
AGGAAAGTTGGAGCTGAGCC+TGG 0.410627 5:+7726653 None:intergenic
CAAAGAAAAGTGAGACTTTG+TGG 0.422343 5:-7724351 MsG0580024650.01.T01:CDS
CCTACACTTCGTGAACTTCA+AGG 0.446024 5:-7724509 MsG0580024650.01.T01:CDS
GGCACTAGCCCGCCTAGCTG+GGG 0.470886 5:+7726675 None:intergenic
ATGATATTAAAGAGAACAAA+AGG 0.473052 5:+7726725 None:intergenic
AAGGTGAATGAGTGTCGTTT+GGG 0.474876 5:-7726800 None:intergenic
GGCTGGCTACAAAGGAAAGT+TGG 0.477787 5:+7726641 None:intergenic
GCTAAAGTTGTAGTAATCTC+AGG 0.482974 5:+7726556 None:intergenic
AAGGAACAAGTCTAGTAAAT+GGG 0.492795 5:-7726770 MsG0580024650.01.T01:CDS
ATATCATTTGGAGTAGTACC+AGG 0.492809 5:-7726709 MsG0580024650.01.T01:CDS
TGGGATGGGCAACTTGATCT+TGG 0.516052 5:-7726751 MsG0580024650.01.T01:CDS
AGTTGTAGTAATGGCTGGGC+TGG 0.517562 5:+7726624 None:intergenic
GTAATCTCAGGAAAATCTGC+AGG 0.532546 5:+7726568 None:intergenic
AAAGCTCAAAGTTGTAGTAA+TGG 0.535973 5:+7726615 None:intergenic
CCTTGAAGTTCACGAAGTGT+AGG 0.544038 5:+7724509 None:intergenic
AATGGCTGGGCTGGCTACAA+AGG 0.546117 5:+7726633 None:intergenic
GCACTAGCCCGCCTAGCTGG+GGG 0.548183 5:+7726676 None:intergenic
GTGTCGTTTGGGTATTTGCA+AGG 0.551928 5:-7726789 MsG0580024650.01.T01:CDS
CATCTGGGAACCTAAATCCT+TGG 0.555929 5:+7726455 None:intergenic
AATCCTAGTTTACCTCATCT+GGG 0.558865 5:+7726440 None:intergenic
TGAACTCTACCATAAGAGAA+AGG 0.559158 5:-7724287 MsG0580024650.01.T01:CDS
AGGATTTAGGTTCCCAGATG+AGG 0.562504 5:-7726452 MsG0580024650.01.T01:intron
TGTTTCCATAGGAAAAGCTC+AGG 0.566477 5:+7726496 None:intergenic
CTTCAAGGAAAGATGAGGGA+AGG 0.566524 5:-7724494 MsG0580024650.01.T01:intron
AACAAGTCTAGTAAATGGGA+TGG 0.577188 5:-7726766 MsG0580024650.01.T01:CDS
TCAAAGTTGTAGTAATGGCT+GGG 0.579354 5:+7726620 None:intergenic
GGAAAGTTGGAGCTGAGCCT+GGG 0.581145 5:+7726654 None:intergenic
TGGCAAGAAAGTGTTTCCAT+AGG 0.586540 5:+7726485 None:intergenic
GTGAACTTCAAGGAAAGATG+AGG 0.592845 5:-7724499 MsG0580024650.01.T01:CDS
AATTAGCCCAAAACATCCGC+AGG 0.608836 5:+7726591 None:intergenic
ACAAGTCTAGTAAATGGGAT+GGG 0.616658 5:-7726765 MsG0580024650.01.T01:CDS
AGAATTAGCCCAAAATCCGC+AGG 0.618781 5:+7726528 None:intergenic
TATCATTTGGAGTAGTACCA+GGG 0.630641 5:-7726708 MsG0580024650.01.T01:CDS
TGAACTTCAAGGAAAGATGA+GGG 0.638807 5:-7724498 MsG0580024650.01.T01:CDS
AGCTGGAATGACCCACATGA+TGG 0.649702 5:-7724374 MsG0580024650.01.T01:CDS
ACAACTTTAGCTTTCTCCTG+CGG 0.656337 5:-7726544 MsG0580024650.01.T01:CDS
ATCATTTGGAGTAGTACCAG+GGG 0.680624 5:-7726707 MsG0580024650.01.T01:CDS
ATTTGGAGTAGTACCAGGGG+CGG 0.687947 5:-7726704 MsG0580024650.01.T01:CDS
GGTAGAGTTCAAATACATAG+CGG 0.738599 5:+7724299 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GATATTAAAAATTTCAAATA+TGG + Chr5:7724971-7724990 None:intergenic 10.0%
!!! TAAATAACTAATTTTGTAAA+TGG - Chr5:7725825-7725844 MsG0580024650.01.T01:intron 10.0%
!!! TTTATTTTTTAAAATGTGTT+TGG + Chr5:7724863-7724882 None:intergenic 10.0%
!! AAATGAAAATATGACATATA+AGG + Chr5:7726451-7726470 None:intergenic 15.0%
!! AATCATGATGTAAAAATTTA+GGG + Chr5:7724827-7724846 None:intergenic 15.0%
!! TTAAAAATTTCAAATATGGA+TGG + Chr5:7724967-7724986 None:intergenic 15.0%
!! TTCAAATTATTCAAGAAATT+TGG + Chr5:7725476-7725495 None:intergenic 15.0%
!! TTGTCAACAAAAAAAAAAAA+AGG - Chr5:7725037-7725056 MsG0580024650.01.T01:intron 15.0%
!!! ATTTTTTATTGTGATTTCTT+TGG - Chr5:7724770-7724789 MsG0580024650.01.T01:intron 15.0%
!!! TAAAATAGTTTGTAAATTAG+AGG + Chr5:7724939-7724958 None:intergenic 15.0%
!!! TTATTATTTCTAAGGAATAA+CGG - Chr5:7724902-7724921 MsG0580024650.01.T01:intron 15.0%
!! AAGCTTAATTAAAATCACTT+AGG + Chr5:7725337-7725356 None:intergenic 20.0%
!! ATGATATTAAAGAGAACAAA+AGG + Chr5:7724326-7724345 None:intergenic 20.0%
!! GAATCATGATGTAAAAATTT+AGG + Chr5:7724828-7724847 None:intergenic 20.0%
!! GATTTCTTTGGTTAAAATTT+GGG - Chr5:7724782-7724801 MsG0580024650.01.T01:intron 20.0%
!! GTTATTCCTTAGAAATAATA+AGG + Chr5:7724903-7724922 None:intergenic 20.0%
!! TAACACATAAACAACAATTA+GGG + Chr5:7725794-7725813 None:intergenic 20.0%
!! TCTTATCAATTGTATATACT+TGG + Chr5:7726227-7726246 None:intergenic 20.0%
!! TGATTTCTTTGGTTAAAATT+TGG - Chr5:7724781-7724800 MsG0580024650.01.T01:intron 20.0%
!! TTAACACATAAACAACAATT+AGG + Chr5:7725795-7725814 None:intergenic 20.0%
!! TTGTTCTCTTTAATATCATT+TGG - Chr5:7724327-7724346 MsG0580024650.01.T01:CDS 20.0%
!!! AATGATCCTTATTATTTCTA+AGG - Chr5:7724894-7724913 MsG0580024650.01.T01:intron 20.0%
!!! CTGTTCTTCAATATTTTTTA+GGG - Chr5:7725308-7725327 MsG0580024650.01.T01:intron 20.0%
!!! CTTTTTTTCACAAATCAAAT+AGG - Chr5:7725008-7725027 MsG0580024650.01.T01:intron 20.0%
! AAAACAAAGATACCTTCAAA+TGG + Chr5:7724681-7724700 None:intergenic 25.0%
! ACTGGTATTATTGGATAAAA+TGG - Chr5:7726127-7726146 MsG0580024650.01.T01:intron 25.0%
! AGTAACAAATGGAACTTTAA+AGG + Chr5:7726405-7726424 None:intergenic 25.0%
! ATATTGTTGTATCCTTTATG+TGG - Chr5:7726650-7726669 MsG0580024650.01.T01:CDS 25.0%
! ATTCAAGAAATTTGGATACA+CGG + Chr5:7725468-7725487 None:intergenic 25.0%
! CCTAAAAAATATTGAAGAAC+AGG + Chr5:7725310-7725329 None:intergenic 25.0%
! CTGGTATTATTGGATAAAAT+GGG - Chr5:7726128-7726147 MsG0580024650.01.T01:intron 25.0%
! GAATGAAAAAGAATACAGTA+AGG + Chr5:7726606-7726625 None:intergenic 25.0%
! TAGAAATTTCCTTTCTCTTA+TGG + Chr5:7726773-7726792 None:intergenic 25.0%
! TATTATTCATAAGCTTGTCA+AGG + Chr5:7725721-7725740 None:intergenic 25.0%
! TCTATTCTACTTATGTTAAG+AGG + Chr5:7726358-7726377 None:intergenic 25.0%
! TCTTACTCTTTGTAAGTTTA+GGG - Chr5:7725086-7725105 MsG0580024650.01.T01:intron 25.0%
! TGGTATTATTGGATAAAATG+GGG - Chr5:7726129-7726148 MsG0580024650.01.T01:intron 25.0%
!! ATTATAAACTAGGGTTTGTT+TGG - Chr5:7725581-7725600 MsG0580024650.01.T01:intron 25.0%
!! ATTTTATGCACTAGATGATA+TGG - Chr5:7724724-7724743 MsG0580024650.01.T01:intron 25.0%
!! GTTTAAAACGTAAAGAGAAA+AGG + Chr5:7725372-7725391 None:intergenic 25.0%
!! TGGTGTGCAATTTTAAATAT+AGG + Chr5:7726723-7726742 None:intergenic 25.0%
!! TTATAAACTAGGGTTTGTTT+GGG - Chr5:7725582-7725601 MsG0580024650.01.T01:intron 25.0%
!! TTTTATCCAATAATACCAGT+TGG + Chr5:7726127-7726146 None:intergenic 25.0%
!! TTTTCACAAATCAAATAGGT+AGG - Chr5:7725012-7725031 MsG0580024650.01.T01:intron 25.0%
!!! CCTGTTCTTCAATATTTTTT+AGG - Chr5:7725307-7725326 MsG0580024650.01.T01:intron 25.0%
AAAAAGAACGAAAAAAGGGT+TGG - Chr5:7725062-7725081 MsG0580024650.01.T01:intron 30.0%
AAAAATATTGAAGAACAGGC+AGG + Chr5:7725306-7725325 None:intergenic 30.0%
AAAAGAACGAAAAAAGGGTT+GGG - Chr5:7725063-7725082 MsG0580024650.01.T01:intron 30.0%
AAAGCTCAAAGTTGTAGTAA+TGG + Chr5:7724436-7724455 None:intergenic 30.0%
AACAGCTTATGCAAAAAAGA+AGG - Chr5:7725629-7725648 MsG0580024650.01.T01:intron 30.0%
AAGGAACAAGTCTAGTAAAT+GGG - Chr5:7724278-7724297 MsG0580024650.01.T01:CDS 30.0%
ACAGCTTATGCAAAAAAGAA+GGG - Chr5:7725630-7725649 MsG0580024650.01.T01:intron 30.0%
ACATTCTAGCAAGTAACAAA+TGG + Chr5:7726416-7726435 None:intergenic 30.0%
AGGAGAAAAAGAACGAAAAA+AGG - Chr5:7725057-7725076 MsG0580024650.01.T01:intron 30.0%
AGGGCACAAATTTAGATTTA+GGG + Chr5:7724808-7724827 None:intergenic 30.0%
AGTTAGGTTAAGTTAGTTAG+TGG + Chr5:7726306-7726325 None:intergenic 30.0%
CAAACCCTAGTTTATAATGT+TGG + Chr5:7725579-7725598 None:intergenic 30.0%
GGAGAAAAAGAACGAAAAAA+GGG - Chr5:7725058-7725077 MsG0580024650.01.T01:intron 30.0%
GTCTTACTCTTTGTAAGTTT+AGG - Chr5:7725085-7725104 MsG0580024650.01.T01:intron 30.0%
TAGGGCACAAATTTAGATTT+AGG + Chr5:7724809-7724828 None:intergenic 30.0%
TCAGAAAAAGTCAATCCAAA+CGG - Chr5:7725767-7725786 MsG0580024650.01.T01:intron 30.0%
TGTGCCAACATTATAAACTA+GGG - Chr5:7725572-7725591 MsG0580024650.01.T01:intron 30.0%
TTTAATTAAGCTTATGCTCG+TGG - Chr5:7725344-7725363 MsG0580024650.01.T01:intron 30.0%
TTTATCGTATAAGTACTCAC+TGG - Chr5:7725162-7725181 MsG0580024650.01.T01:intron 30.0%
TTTCTTATGATGTCATGTTG+TGG - Chr5:7725514-7725533 MsG0580024650.01.T01:intron 30.0%
! ACAAGCTATGAAGTGTTTAT+AGG + Chr5:7726381-7726400 None:intergenic 30.0%
! AGTACGATGCTTATTTCTTA+AGG - Chr5:7725419-7725438 MsG0580024650.01.T01:intron 30.0%
!!! AGGTTTTGAATCATCAACAA+TGG - Chr5:7725439-7725458 MsG0580024650.01.T01:intron 30.0%
AAATCCTAGTTTACCTCATC+TGG + Chr5:7724612-7724631 None:intergenic 35.0%
AACAAGTCTAGTAAATGGGA+TGG - Chr5:7724282-7724301 MsG0580024650.01.T01:CDS 35.0%
AAGAATGAGAAGACAAGTAG+TGG + Chr5:7725194-7725213 None:intergenic 35.0%
AAGAGTTCACTTGGTAAGTT+AGG + Chr5:7726322-7726341 None:intergenic 35.0%
AATCCTAGTTTACCTCATCT+GGG + Chr5:7724611-7724630 None:intergenic 35.0%
ACAAGTCTAGTAAATGGGAT+GGG - Chr5:7724283-7724302 MsG0580024650.01.T01:CDS 35.0%
ACATGTTGCCTATAGATAAG+AGG + Chr5:7726270-7726289 None:intergenic 35.0%
ATAAGATAGCTCACTCTCTA+AGG - Chr5:7726241-7726260 MsG0580024650.01.T01:intron 35.0%
CAAAGAAAAGTGAGACTTTG+TGG - Chr5:7726697-7726716 MsG0580024650.01.T01:CDS 35.0%
CAAGGAACAAGTCTAGTAAA+TGG - Chr5:7724277-7724296 MsG0580024650.01.T01:CDS 35.0%
CAGAAAAAGTCAATCCAAAC+GGG - Chr5:7725768-7725787 MsG0580024650.01.T01:intron 35.0%
CATGTTGCCTATAGATAAGA+GGG + Chr5:7726269-7726288 None:intergenic 35.0%
CTTGTCAAGGTAGTTTATGA+AGG + Chr5:7725708-7725727 None:intergenic 35.0%
GAGTAGTACAATCCATTTGA+AGG - Chr5:7724666-7724685 MsG0580024650.01.T01:intron 35.0%
GCTAAAGTTGTAGTAATCTC+AGG + Chr5:7724495-7724514 None:intergenic 35.0%
GGTAGAGTTCAAATACATAG+CGG + Chr5:7726752-7726771 None:intergenic 35.0%
GTAGTTAAGAAGAGTTCACT+TGG + Chr5:7726331-7726350 None:intergenic 35.0%
GTGTGCCAACATTATAAACT+AGG - Chr5:7725571-7725590 MsG0580024650.01.T01:intron 35.0%
TCAAAGTTGTAGTAATGGCT+GGG + Chr5:7724431-7724450 None:intergenic 35.0%
TCGAAACCAACTGGTATTAT+TGG - Chr5:7726118-7726137 MsG0580024650.01.T01:intron 35.0%
TGAACTCTACCATAAGAGAA+AGG - Chr5:7726761-7726780 MsG0580024650.01.T01:CDS 35.0%
TGAACTTCAAGGAAAGATGA+GGG - Chr5:7726550-7726569 MsG0580024650.01.T01:CDS 35.0%
TGTCTTCTCATTCTTCTTCT+CGG - Chr5:7725199-7725218 MsG0580024650.01.T01:intron 35.0%
! ACTTTTCTTTGCCATCATGT+GGG + Chr5:7726688-7726707 None:intergenic 35.0%
! ATATCATTTGGAGTAGTACC+AGG - Chr5:7724339-7724358 MsG0580024650.01.T01:CDS 35.0%
! ATTTTCCTGAGCTTTTCCTA+TGG - Chr5:7724547-7724566 MsG0580024650.01.T01:CDS 35.0%
! TATCATTTGGAGTAGTACCA+GGG - Chr5:7724340-7724359 MsG0580024650.01.T01:CDS 35.0%
! TGTATATACTTGGAGCCTTT+TGG + Chr5:7726217-7726236 None:intergenic 35.0%
!! AAATGGATTGTACTACTCAG+TGG + Chr5:7724664-7724683 None:intergenic 35.0%
!!! GACTTGATAGCTAGCTTTTA+AGG + Chr5:7725896-7725915 None:intergenic 35.0%
!!! TGATAGCTAGCTTTTAAGGA+GGG + Chr5:7725892-7725911 None:intergenic 35.0%
!!! TTGATAGCTAGCTTTTAAGG+AGG + Chr5:7725893-7725912 None:intergenic 35.0%
AAATTCCACATCAGGCAGTA+TGG + Chr5:7725943-7725962 None:intergenic 40.0%
AATTCCACATCAGGCAGTAT+GGG + Chr5:7725942-7725961 None:intergenic 40.0%
AGTTAAAAACCCAGAAGGCT+GGG - Chr5:7726505-7726524 MsG0580024650.01.T01:CDS 40.0%
CAATTAATTCCCAGCCTTCT+GGG + Chr5:7726517-7726536 None:intergenic 40.0%
CCTAAATCCTTGGCAAAATC+TGG + Chr5:7724586-7724605 None:intergenic 40.0%
CTAGAGTCTCTCTAATACTC+AGG + Chr5:7726182-7726201 None:intergenic 40.0%
CTCAAAGTTGTAGTAATGGC+TGG + Chr5:7724432-7724451 None:intergenic 40.0%
CTTATGATGTCATGTTGTGG+TGG - Chr5:7725517-7725536 MsG0580024650.01.T01:intron 40.0%
GTAATCTCAGGAAAATCTGC+AGG + Chr5:7724483-7724502 None:intergenic 40.0%
GTGAACTTCAAGGAAAGATG+AGG - Chr5:7726549-7726568 MsG0580024650.01.T01:CDS 40.0%
TAAGGCTCCCTCTTATCTAT+AGG - Chr5:7726259-7726278 MsG0580024650.01.T01:intron 40.0%
TCAATTAATTCCCAGCCTTC+TGG + Chr5:7726518-7726537 None:intergenic 40.0%
TGTTTCCATAGGAAAAGCTC+AGG + Chr5:7724555-7724574 None:intergenic 40.0%
! ACAACTTTGAGCTTTTCCTG+CGG - Chr5:7724441-7724460 MsG0580024650.01.T01:intron 40.0%
! ATCATTTGGAGTAGTACCAG+GGG - Chr5:7724341-7724360 MsG0580024650.01.T01:CDS 40.0%
! CACTTTTCTTTGCCATCATG+TGG + Chr5:7726689-7726708 None:intergenic 40.0%
! CCAGATTTTGCCAAGGATTT+AGG - Chr5:7724583-7724602 MsG0580024650.01.T01:intron 40.0%
! TTGTAAGTTTAGGGCGTGTT+TGG - Chr5:7725095-7725114 MsG0580024650.01.T01:intron 40.0%
! TTTCTTGCCAGATTTTGCCA+AGG - Chr5:7724576-7724595 MsG0580024650.01.T01:intron 40.0%
!! ACAACTTTAGCTTTCTCCTG+CGG - Chr5:7724504-7724523 MsG0580024650.01.T01:CDS 40.0%
!! ACTCGGCTTGTCTTTACTTT+TGG + Chr5:7726488-7726507 None:intergenic 40.0%
!! AGCCTTCTGGGTTTTTAACT+CGG + Chr5:7726505-7726524 None:intergenic 40.0%
!! TGGCAAGAAAGTGTTTCCAT+AGG + Chr5:7724566-7724585 None:intergenic 40.0%
!!! ATAGCTAGCTTTTAAGGAGG+GGG + Chr5:7725890-7725909 None:intergenic 40.0%
!!! GATAGCTAGCTTTTAAGGAG+GGG + Chr5:7725891-7725910 None:intergenic 40.0%
AACAACAATTAGGGCCCGTT+TGG + Chr5:7725785-7725804 None:intergenic 45.0%
AATTAGCCCAAAACATCCGC+AGG + Chr5:7724460-7724479 None:intergenic 45.0%
ACGACAATAGCAAAGGTGAG+CGG + Chr5:7725271-7725290 None:intergenic 45.0%
AGAATTAGCCCAAAATCCGC+AGG + Chr5:7724523-7724542 None:intergenic 45.0%
AGCCGAGTTAAAAACCCAGA+AGG - Chr5:7726500-7726519 MsG0580024650.01.T01:CDS 45.0%
ATTCTTCTTCTCGGTTGTCG+AGG - Chr5:7725208-7725227 MsG0580024650.01.T01:intron 45.0%
CAAATACATAGCGGCTCTTC+TGG + Chr5:7726743-7726762 None:intergenic 45.0%
CATCTGGGAACCTAAATCCT+TGG + Chr5:7724596-7724615 None:intergenic 45.0%
CCTACACTTCGTGAACTTCA+AGG - Chr5:7726539-7726558 MsG0580024650.01.T01:CDS 45.0%
CCTTGAAGTTCACGAAGTGT+AGG + Chr5:7726542-7726561 None:intergenic 45.0%
CTTCAAGGAAAGATGAGGGA+AGG - Chr5:7726554-7726573 MsG0580024650.01.T01:CDS 45.0%
GAGTTAAAAACCCAGAAGGC+TGG - Chr5:7726504-7726523 MsG0580024650.01.T01:CDS 45.0%
GTTCCCAGATGAGGTAAACT+AGG - Chr5:7724605-7724624 MsG0580024650.01.T01:intron 45.0%
TAGACTCTCCCAATGCCAAA+AGG - Chr5:7726199-7726218 MsG0580024650.01.T01:intron 45.0%
TCACTCTACAACGCTGTACT+TGG - Chr5:7726026-7726045 MsG0580024650.01.T01:intron 45.0%
TCATTCCAGCTGCCACATAA+AGG + Chr5:7726665-7726684 None:intergenic 45.0%
TCCAGTTGATCGAAACCAAC+TGG - Chr5:7726109-7726128 MsG0580024650.01.T01:intron 45.0%
TGGATTGTACTACTCAGTGG+CGG + Chr5:7724661-7724680 None:intergenic 45.0%
TGTATCCTTTATGTGGCAGC+TGG - Chr5:7726657-7726676 MsG0580024650.01.T01:CDS 45.0%
! ACCAGTTGGTTTCGATCAAC+TGG + Chr5:7726113-7726132 None:intergenic 45.0%
! ACTTGGAGCCTTTTGGCATT+GGG + Chr5:7726210-7726229 None:intergenic 45.0%
! AGCTTTCTCCTGCGGATTTT+GGG - Chr5:7724512-7724531 MsG0580024650.01.T01:CDS 45.0%
! AGCTTTTCCTGCGGATGTTT+TGG - Chr5:7724450-7724469 MsG0580024650.01.T01:intron 45.0%
! AGGATTTAGGTTCCCAGATG+AGG - Chr5:7724596-7724615 MsG0580024650.01.T01:intron 45.0%
! GCTTTTCCTGCGGATGTTTT+GGG - Chr5:7724451-7724470 MsG0580024650.01.T01:intron 45.0%
! GGTAAACTAGGATTTTGCCC+AGG - Chr5:7724617-7724636 MsG0580024650.01.T01:intron 45.0%
! GTTTAGGGCGTGTTTGGTTA+CGG - Chr5:7725101-7725120 MsG0580024650.01.T01:intron 45.0%
! TACTTGGAGCCTTTTGGCAT+TGG + Chr5:7726211-7726230 None:intergenic 45.0%
! TAGCTTTCTCCTGCGGATTT+TGG - Chr5:7724511-7724530 MsG0580024650.01.T01:CDS 45.0%
!! ACCTTTGCTATTGTCGTCTG+AGG - Chr5:7725274-7725293 MsG0580024650.01.T01:intron 45.0%
!! CCTTTGCTATTGTCGTCTGA+GGG - Chr5:7725275-7725294 MsG0580024650.01.T01:intron 45.0%
!! GTGTCGTTTGGGTATTTGCA+AGG - Chr5:7724259-7724278 MsG0580024650.01.T01:CDS 45.0%
!!! TAGCTAGCTTTTAAGGAGGG+GGG + Chr5:7725889-7725908 None:intergenic 45.0%
ACAATAGCAAAGGTGAGCGG+TGG + Chr5:7725268-7725287 None:intergenic 50.0%
AGCTGGAATGACCCACATGA+TGG - Chr5:7726674-7726693 MsG0580024650.01.T01:CDS 50.0%
AGGCAGTATGGGATGCTCAA+TGG + Chr5:7725931-7725950 None:intergenic 50.0%
AGTTGTAGTAATGGCTGGGC+TGG + Chr5:7724427-7724446 None:intergenic 50.0%
CCCTCAGACGACAATAGCAA+AGG + Chr5:7725278-7725297 None:intergenic 50.0%
CGATCAACTGGAATCAGAGC+TGG + Chr5:7726101-7726120 None:intergenic 50.0%
CGCAAGTCAGTTCTCATGGA+CGG + Chr5:7725999-7726018 None:intergenic 50.0%
CTGGAATCAGAGCTGGTTGT+TGG + Chr5:7726094-7726113 None:intergenic 50.0%
GAATCAGAGCTGGTTGTTGG+TGG + Chr5:7726091-7726110 None:intergenic 50.0%
GTGCACGCAAATTCCACATC+AGG + Chr5:7725951-7725970 None:intergenic 50.0%
TGGGATGGGCAACTTGATCT+TGG - Chr5:7724297-7724316 MsG0580024650.01.T01:CDS 50.0%
TGTCCGCAAGTCAGTTCTCA+TGG + Chr5:7726003-7726022 None:intergenic 50.0%
! AACGCTGTACTTGGCCTTTC+TGG - Chr5:7726035-7726054 MsG0580024650.01.T01:intron 50.0%
! ATTTGGAGTAGTACCAGGGG+CGG - Chr5:7724344-7724363 MsG0580024650.01.T01:CDS 50.0%
! GGCTGGCTACAAAGGAAAGT+TGG + Chr5:7724410-7724429 None:intergenic 50.0%
! TGCTCAATGGAGTGTCTGAG+TGG + Chr5:7725918-7725937 None:intergenic 50.0%
!! CGTCCATGAGAACTGACTTG+CGG - Chr5:7725997-7726016 MsG0580024650.01.T01:intron 50.0%
!! TCATGGACGGTGCTACAGTA+GGG + Chr5:7725986-7726005 None:intergenic 50.0%
!!! AGCCAGGATTTTCTAGAGCC+TGG + Chr5:7724638-7724657 None:intergenic 50.0%
!!! GCCAGGATTTTCTAGAGCCT+GGG + Chr5:7724637-7724656 None:intergenic 50.0%
AGGAAAGTTGGAGCTGAGCC+TGG + Chr5:7724398-7724417 None:intergenic 55.0%
GAGGTAAACTCCGTCGCTTC+CGG - Chr5:7725227-7725246 MsG0580024650.01.T01:intron 55.0%
GCATCCCATACTGCCTGATG+TGG - Chr5:7725935-7725954 MsG0580024650.01.T01:intron 55.0%
GGAAAGTTGGAGCTGAGCCT+GGG + Chr5:7724397-7724416 None:intergenic 55.0%
TACGGAGGAGCTGACCAGAA+AGG + Chr5:7726052-7726071 None:intergenic 55.0%
TAGCAAAGGTGAGCGGTGGT+TGG + Chr5:7725264-7725283 None:intergenic 55.0%
! TTTCTGGTCAGCTCCTCCGT+AGG - Chr5:7726051-7726070 MsG0580024650.01.T01:intron 55.0%
!! AATGGCTGGGCTGGCTACAA+AGG + Chr5:7724418-7724437 None:intergenic 55.0%
!! ACGGTGCTACAGTAGGGGTT+GGG + Chr5:7725980-7725999 None:intergenic 55.0%
!! AGAGCTGGTTGTTGGTGGCT+CGG + Chr5:7726086-7726105 None:intergenic 55.0%
!! CATGGACGGTGCTACAGTAG+GGG + Chr5:7725985-7726004 None:intergenic 55.0%
!! CTCATGGACGGTGCTACAGT+AGG + Chr5:7725987-7726006 None:intergenic 55.0%
!! GCCCAGGCTCTAGAAAATCC+TGG - Chr5:7724633-7724652 MsG0580024650.01.T01:intron 55.0%
!! GGAGGGGGGTACTTATCAGT+TGG + Chr5:7725875-7725894 None:intergenic 55.0%
!! GGTTGTTGGTGGCTCGGAAA+GGG + Chr5:7726080-7726099 None:intergenic 55.0%
!! TGGTTGTTGGTGGCTCGGAA+AGG + Chr5:7726081-7726100 None:intergenic 55.0%
GGCTCGGAAAGGGCTACCTA+CGG + Chr5:7726070-7726089 None:intergenic 60.0%
TCGGAAAGGGCTACCTACGG+AGG + Chr5:7726067-7726086 None:intergenic 60.0%
! GTGGTTGGTCGCACAACTGC+CGG + Chr5:7725249-7725268 None:intergenic 60.0%
! TACTACTCAGTGGCGGAGCC+AGG + Chr5:7724654-7724673 None:intergenic 60.0%
!! GACGGTGCTACAGTAGGGGT+TGG + Chr5:7725981-7726000 None:intergenic 60.0%
CGCACAACTGCCGGAAGCGA+CGG + Chr5:7725240-7725259 None:intergenic 65.0%
GCACTAGCCCGCCTAGCTGG+GGG + Chr5:7724375-7724394 None:intergenic 70.0%
GGCACTAGCCCGCCTAGCTG+GGG + Chr5:7724376-7724395 None:intergenic 70.0%
GGGCACTAGCCCGCCTAGCT+GGG + Chr5:7724377-7724396 None:intergenic 70.0%
TGGGCACTAGCCCGCCTAGC+TGG + Chr5:7724378-7724397 None:intergenic 70.0%
!! AGCTAGGCGGGCTAGTGCCC+AGG - Chr5:7724377-7724396 MsG0580024650.01.T01:intron 70.0%
CGGAGCCACCCCCAGCTAGG+CGG - Chr5:7724364-7724383 MsG0580024650.01.T01:CDS 75.0%
CTAGCCCGCCTAGCTGGGGG+TGG + Chr5:7724372-7724391 None:intergenic 75.0%
GGAGCCACCCCCAGCTAGGC+GGG - Chr5:7724365-7724384 MsG0580024650.01.T01:CDS 75.0%
! GGGCGGAGCCACCCCCAGCT+AGG - Chr5:7724361-7724380 MsG0580024650.01.T01:CDS 80.0%
! GCTGGGGGTGGCTCCGCCCC+TGG + Chr5:7724360-7724379 None:intergenic 85.0%
Chromosome Type Strat End Strand Name
Chr5 gene 7724255 7726815 7724255 ID=MsG0580024650.01;Name=MsG0580024650.01
Chr5 mRNA 7724255 7726815 7724255 ID=MsG0580024650.01.T01;Parent=MsG0580024650.01;Name=MsG0580024650.01.T01;_AED=0.49;_eAED=0.80;_QI=0|0|0|1|1|1|3|0|200
Chr5 exon 7726453 7726815 7726453 ID=MsG0580024650.01.T01:exon:24419;Parent=MsG0580024650.01.T01
Chr5 exon 7724495 7724594 7724495 ID=MsG0580024650.01.T01:exon:24420;Parent=MsG0580024650.01.T01
Chr5 exon 7724255 7724394 7724255 ID=MsG0580024650.01.T01:exon:24421;Parent=MsG0580024650.01.T01
Chr5 CDS 7726453 7726815 7726453 ID=MsG0580024650.01.T01:cds;Parent=MsG0580024650.01.T01
Chr5 CDS 7724495 7724594 7724495 ID=MsG0580024650.01.T01:cds;Parent=MsG0580024650.01.T01
Chr5 CDS 7724255 7724394 7724255 ID=MsG0580024650.01.T01:cds;Parent=MsG0580024650.01.T01
Gene Sequence

>MsG0580024650.01.T01

ATGAGTGTCGTTTGGGTATTTGCAAGGAACAAGTCTAGTAAATGGGATGGGCAACTTGATCTTGGTGACCTTTTGTTCTCTTTAATATCATTTGGAGTAGTACCAGGGGCGGAGCCACCCCCAGCTAGGCGGGCTAGTGCCCAGGCTCAGCTCCAACTTTCCTTTGTAGCCAGCCCAGCCATTACTACAACTTTGAGCTTTTCCTGCGGATGTTTTGGGCTAATTCCTGCAGATTTTCCTGAGATTACTACAACTTTAGCTTTCTCCTGCGGATTTTGGGCTAATTCTGCAGATTTTCCTGAGCTTTTCCTATGGAAACACTTTCTTGCCAGATTTTGCCAAGGATTTAGGTTCCCAGATGAGATAAAAATGCCAAAAGTAAAGACAAGCCGAGTTAAAAACCCAGAAGGCTGGGAATTAATTGAGCCTACACTTCGTGAACTTCAAGGAAAGATGAGGGAAGCTGGAATGACCCACATGATGGCAAAGAAAAGTGAGACTTTGTGGCCTATATTTAAAATTGCACACCAGAAGAGCCGCTATGTATTTGAACTCTACCATAAGAGAAAGGAAATTTCTAAAGAATTGTATGAATTCTGTTAG
Protein sequence

>MsG0580024650.01.T01

MSVVWVFARNKSSKWDGQLDLGDLLFSLISFGVVPGAEPPPARRASAQAQLQLSFVASPAITTTLSFSCGCFGLIPADFPEITTTLAFSCGFWANSADFPELFLWKHFLARFCQGFRFPDEIKMPKVKTSRVKNPEGWELIEPTLRELQGKMREAGMTHMMAKKSETLWPIFKIAHQKSRYVFELYHKRKEISKELYEFC*