Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780037142.01.T01 | XP_013447974.1 | 100 | 69 | 0 | 0 | 1 | 69 | 1 | 69 | 1.02E-40 | 138 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780037142.01.T01 | I3SYL4 | 100.000 | 69 | 0 | 0 | 1 | 69 | 1 | 69 | 4.87e-41 | 138 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048058.01 | MsG0780037142.01 | 0.822279 | 2.578583e-53 | 2.148580e-50 |
| MsG0080048461.01 | MsG0780037142.01 | 0.820899 | 5.375907e-53 | 4.308617e-50 |
| MsG0180003652.01 | MsG0780037142.01 | 0.822942 | 1.808468e-53 | 1.535506e-50 |
| MsG0180004005.01 | MsG0780037142.01 | 0.801680 | 8.033381e-49 | 3.859776e-46 |
| MsG0180004295.01 | MsG0780037142.01 | 0.812915 | 3.333865e-51 | 2.146464e-48 |
| MsG0180004331.01 | MsG0780037142.01 | 0.802342 | 5.871211e-49 | 2.869073e-46 |
| MsG0280010560.01 | MsG0780037142.01 | 0.806186 | 9.295915e-50 | 5.013406e-47 |
| MsG0380012020.01 | MsG0780037142.01 | 0.809823 | 1.563958e-50 | 9.276784e-48 |
| MsG0380014537.01 | MsG0780037142.01 | 0.804067 | 2.581256e-49 | 1.318334e-46 |
| MsG0680034037.01 | MsG0780037142.01 | 0.816842 | 4.490157e-52 | 3.217052e-49 |
| MsG0480023025.01 | MsG0780037142.01 | 0.831033 | 2.099897e-55 | 2.251403e-52 |
| MsG0480023165.01 | MsG0780037142.01 | 0.836356 | 9.819779e-57 | 1.234543e-53 |
| MsG0480023224.01 | MsG0780037142.01 | 0.814985 | 1.165842e-51 | 7.938869e-49 |
| MsG0480023873.01 | MsG0780037142.01 | 0.800185 | 1.622939e-48 | 7.508277e-46 |
| MsG0380016348.01 | MsG0780037142.01 | 0.815856 | 7.460299e-52 | 5.202502e-49 |
| MsG0380017140.01 | MsG0780037142.01 | 0.809838 | 1.552127e-50 | 9.210445e-48 |
| MsG0680030447.01 | MsG0780037142.01 | 0.810401 | 1.174325e-50 | 7.071334e-48 |
| MsG0280007648.01 | MsG0780037142.01 | 0.800201 | 1.610540e-48 | 7.453896e-46 |
| MsG0280008039.01 | MsG0780037142.01 | 0.819388 | 1.192548e-52 | 9.163478e-50 |
| MsG0280008615.01 | MsG0780037142.01 | 0.819028 | 1.440086e-52 | 1.095752e-49 |
| MsG0280010233.01 | MsG0780037142.01 | 0.812818 | 3.500579e-51 | 2.247922e-48 |
| MsG0680035773.01 | MsG0780037142.01 | 0.800700 | 1.274571e-48 | 5.974060e-46 |
| MsG0780037141.01 | MsG0780037142.01 | 0.821131 | 4.751445e-53 | 3.832857e-50 |
| MsG0780037142.01 | MsG0780040344.01 | 0.830722 | 2.502683e-55 | 2.658971e-52 |
| MsG0780037142.01 | MsG0780041065.01 | 0.810915 | 9.091832e-51 | 5.550970e-48 |
| MsG0780037142.01 | MsG0780041498.01 | 0.805195 | 1.501075e-49 | 7.891923e-47 |
| MsG0780037142.01 | MsG0880043804.01 | 0.829101 | 6.215919e-55 | 6.298905e-52 |
| MsG0780037142.01 | MsG0880046027.01 | 0.804180 | 2.445118e-49 | 1.252350e-46 |
| MsG0780037142.01 | MsG0880047742.01 | 0.809468 | 1.864478e-50 | 1.095548e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780037142.01.T01 | MTR_7g028945 | 100.000 | 69 | 0 | 0 | 1 | 69 | 1 | 69 | 1.23e-44 | 138 |
| MsG0780037142.01.T01 | MTR_8g096840 | 44.776 | 67 | 37 | 0 | 1 | 67 | 1 | 67 | 9.18e-18 | 70.9 |
| MsG0780037142.01.T01 | MTR_3g085020 | 46.970 | 66 | 35 | 0 | 1 | 66 | 1 | 66 | 1.21e-17 | 70.5 |
| MsG0780037142.01.T01 | MTR_8g097020 | 42.647 | 68 | 39 | 0 | 1 | 68 | 1 | 68 | 3.05e-17 | 69.7 |
| MsG0780037142.01.T01 | MTR_8g027820 | 36.264 | 91 | 53 | 1 | 1 | 86 | 1 | 91 | 1.04e-15 | 66.6 |
| MsG0780037142.01.T01 | MTR_3g085030 | 42.647 | 68 | 39 | 0 | 1 | 68 | 1 | 68 | 1.56e-15 | 65.1 |
| MsG0780037142.01.T01 | MTR_7g028975 | 38.806 | 67 | 41 | 0 | 1 | 67 | 1 | 67 | 4.03e-14 | 61.6 |
| MsG0780037142.01.T01 | MTR_7g028940 | 40.299 | 67 | 40 | 0 | 1 | 67 | 1 | 67 | 1.84e-13 | 60.1 |
| MsG0780037142.01.T01 | MTR_7g028815 | 52.941 | 51 | 24 | 0 | 1 | 51 | 1 | 51 | 5.58e-13 | 60.1 |
| MsG0780037142.01.T01 | MTR_7g028870 | 35.385 | 65 | 42 | 0 | 3 | 67 | 4 | 68 | 1.29e-12 | 57.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780037142.01.T01 | AT5G40960 | 71.642 | 67 | 19 | 0 | 1 | 67 | 1 | 67 | 9.97e-25 | 88.6 |
| MsG0780037142.01.T01 | AT3G48660 | 43.284 | 67 | 38 | 0 | 1 | 67 | 1 | 67 | 1.32e-16 | 68.6 |
| MsG0780037142.01.T01 | AT5G08391 | 42.647 | 68 | 39 | 0 | 1 | 68 | 1 | 68 | 1.40e-16 | 67.8 |
| MsG0780037142.01.T01 | AT5G63500 | 43.284 | 67 | 38 | 0 | 1 | 67 | 1 | 67 | 8.12e-16 | 65.9 |
| MsG0780037142.01.T01 | AT3G27027 | 37.879 | 66 | 41 | 0 | 2 | 67 | 3 | 68 | 1.18e-13 | 60.5 |
| MsG0780037142.01.T01 | AT3G27030 | 40.299 | 67 | 40 | 0 | 1 | 67 | 74 | 140 | 5.15e-13 | 60.8 |
| MsG0780037142.01.T01 | AT3G27030 | 36.364 | 66 | 42 | 0 | 1 | 66 | 1 | 66 | 5.58e-11 | 55.5 |
| MsG0780037142.01.T01 | AT5G40980 | 51.020 | 49 | 24 | 0 | 3 | 51 | 4 | 52 | 5.59e-13 | 58.9 |
| MsG0780037142.01.T01 | AT5G14110 | 37.313 | 67 | 42 | 0 | 3 | 69 | 4 | 70 | 6.82e-13 | 58.5 |
| MsG0780037142.01.T01 | AT3G01950 | 38.462 | 65 | 40 | 0 | 3 | 67 | 4 | 68 | 2.91e-12 | 57.0 |
Find 10 sgRNAs with CRISPR-Local
Find 13 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTGTACACACTGTTCTTTA+TGG | 0.216202 | 7:+20609278 | MsG0780037142.01.T01:CDS |
| AGGGATAGACAATGAATAAT+TGG | 0.218789 | 7:+20609135 | None:intergenic |
| TCATAGCTGCAGCTCTATTT+TGG | 0.248610 | 7:+20609168 | MsG0780037142.01.T01:CDS |
| GGGATAGACAATGAATAATT+GGG | 0.433197 | 7:+20609136 | None:intergenic |
| TCCAACGGTGAATTCTTCCC+TGG | 0.487207 | 7:-20609221 | None:intergenic |
| AACAAGAAAACAAGTATGAA+AGG | 0.535673 | 7:-20609390 | None:intergenic |
| TCCAGGGAAGAATTCACCGT+TGG | 0.558400 | 7:+20609220 | MsG0780037142.01.T01:CDS |
| AATAGAGCTGCAGCTATGAG+AGG | 0.636515 | 7:-20609164 | None:intergenic |
| TTCATGTTCATAAACTCCAA+CGG | 0.642897 | 7:-20609236 | None:intergenic |
| AGAGCTGCAGCTATGAGAGG+AGG | 0.659838 | 7:-20609161 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AACAAGAAAACAAGTATGAA+AGG | - | Chr7:20609393-20609412 | None:intergenic | 25.0% |
| TTCATGTTCATAAACTCCAA+CGG | - | Chr7:20609239-20609258 | None:intergenic | 30.0% | |
| TTTGTACACACTGTTCTTTA+TGG | + | Chr7:20609278-20609297 | MsG0780037142.01.T01:CDS | 30.0% | |
| !! | CATACTTGTTTTCTTGTTCT+TGG | + | Chr7:20609395-20609414 | MsG0780037142.01.T01:CDS | 30.0% |
| !! | GAATGATTTTTCAGCTTCCA+GGG | + | Chr7:20609204-20609223 | MsG0780037142.01.T01:CDS | 35.0% |
| !!! | CTATTTTGGCTATTATCACC+AGG | + | Chr7:20609182-20609201 | MsG0780037142.01.T01:CDS | 35.0% |
| GGAAGCTGAAAAATCATTCC+TGG | - | Chr7:20609203-20609222 | None:intergenic | 40.0% | |
| ! | TCATAGCTGCAGCTCTATTT+TGG | + | Chr7:20609168-20609187 | MsG0780037142.01.T01:CDS | 40.0% |
| !! | GGAATGATTTTTCAGCTTCC+AGG | + | Chr7:20609203-20609222 | MsG0780037142.01.T01:CDS | 40.0% |
| AATAGAGCTGCAGCTATGAG+AGG | - | Chr7:20609167-20609186 | None:intergenic | 45.0% | |
| TCCAACGGTGAATTCTTCCC+TGG | - | Chr7:20609224-20609243 | None:intergenic | 50.0% | |
| TCCAGGGAAGAATTCACCGT+TGG | + | Chr7:20609220-20609239 | MsG0780037142.01.T01:CDS | 50.0% | |
| AGAGCTGCAGCTATGAGAGG+AGG | - | Chr7:20609164-20609183 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 20609146 | 20609424 | 20609146 | ID=MsG0780037142.01;Name=MsG0780037142.01 |
| Chr7 | mRNA | 20609146 | 20609424 | 20609146 | ID=MsG0780037142.01.T01;Parent=MsG0780037142.01;Name=MsG0780037142.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|0|1|0|92 |
| Chr7 | exon | 20609146 | 20609424 | 20609146 | ID=MsG0780037142.01.T01:exon:4109;Parent=MsG0780037142.01.T01 |
| Chr7 | CDS | 20609146 | 20609424 | 20609146 | ID=MsG0780037142.01.T01:cds;Parent=MsG0780037142.01.T01 |
| Gene Sequence |
| Protein sequence |
