AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380017140.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380017140.01.T01 MTR_3g101660 91.045 134 11 1 29 162 789 921 8.50e-78 250
MsG0380017140.01.T01 MTR_3g101660 74.286 70 15 2 1 69 673 740 5.45e-19 84.7
MsG0380017140.01.T01 MTR_1g019490 61.207 116 35 3 43 158 784 889 1.71e-37 137
MsG0380017140.01.T01 MTR_8g070840 51.304 115 44 4 3 116 466 569 6.47e-12 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380017140.01.T01 AT2G26570 45.122 164 73 5 2 160 631 782 7.46e-27 107
MsG0380017140.01.T01 AT2G26570 45.122 164 73 5 2 160 631 782 7.46e-27 107
MsG0380017140.01.T01 AT4G33390 51.613 93 37 2 43 135 667 751 6.23e-16 75.9
MsG0380017140.01.T01 AT5G42880 39.231 130 70 3 5 130 572 696 2.28e-14 71.2
MsG0380017140.01.T01 AT1G45545 42.045 88 50 1 13 100 596 682 3.15e-11 62.0
MsG0380017140.01.T01 AT1G45545 42.045 88 50 1 13 100 597 683 3.24e-11 62.0

Find 46 sgRNAs with CRISPR-Local

Find 83 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATCTTGCCTCCCATCCTTT+TGG 0.256194 3:+92502574 MsG0380017140.01.T01:CDS
GCTGTTAGTTGCAGCTTCTT+TGG 0.261793 3:-92501728 None:intergenic
CGGAGAAAGGCTGGTGAGTC+TGG 0.309159 3:+92502257 MsG0380017140.01.T01:CDS
AAGGCAAAGGAAGGTAAATT+AGG 0.350324 3:+92502191 MsG0380017140.01.T01:CDS
AACAGCTGAAGAGGCTAATC+AGG 0.354780 3:+92501745 MsG0380017140.01.T01:CDS
GTGCAGGATTAGTTGCATAC+TGG 0.374116 3:-92502358 None:intergenic
TTGCAGCTTCTTTGGAAGTT+CGG 0.399807 3:-92501720 None:intergenic
GTACTGGCACAGTAAGCTTT+TGG 0.402796 3:-92502625 None:intergenic
ATAGAGAGATAGCTGCTAGA+AGG 0.425984 3:+92502135 MsG0380017140.01.T01:CDS
TCTGCATGCATATAAGTCTT+CGG 0.426670 3:-92502395 None:intergenic
CAAGGTACTTACTCCAGCCT+TGG 0.427633 3:-92501831 None:intergenic
TATTCTGGTTAACCACTCCT+TGG 0.457248 3:-92502280 None:intergenic
ACTGAAATCCTTCGAGAAGT+TGG 0.462011 3:+92502103 MsG0380017140.01.T01:CDS
TGCAGGATTAGTTGCATACT+GGG 0.468145 3:-92502357 None:intergenic
GTGTAGAACAAGAACTTAGA+AGG 0.477088 3:+92502213 MsG0380017140.01.T01:CDS
CACAATCGATCACCGTGTAC+TGG 0.480463 3:-92502641 None:intergenic
GAAGCAGAACAAGCCAAGGC+TGG 0.481127 3:+92501818 MsG0380017140.01.T01:CDS
TGAAAATGAGCAACGGAGAA+AGG 0.485253 3:+92502244 MsG0380017140.01.T01:CDS
GGACTTGACAAATATTGTGC+AGG 0.492349 3:-92502374 None:intergenic
CACTCAGATCTCCAAAAGGA+TGG 0.503255 3:-92502585 None:intergenic
TAGAGAGATAGCTGCTAGAA+GGG 0.509873 3:+92502136 MsG0380017140.01.T01:CDS
AATGAGCAACGGAGAAAGGC+TGG 0.523536 3:+92502248 MsG0380017140.01.T01:CDS
AAGAAGAATCGTTGTTCCCA+CGG 0.537881 3:+92502464 MsG0380017140.01.T01:CDS
CACTTCATCCAACTTCTCGA+AGG 0.544839 3:-92502111 None:intergenic
AGAAGAATCGTTGTTCCCAC+GGG 0.559267 3:+92502465 MsG0380017140.01.T01:CDS
GATTAGTTGCATACTGGGAC+CGG 0.562727 3:-92502352 None:intergenic
GATCTGAGTGACTCGAATGT+TGG 0.565956 3:+92502598 MsG0380017140.01.T01:CDS
TGAAGAACTGCAGAAAGTAA+AGG 0.567540 3:+92501793 MsG0380017140.01.T01:CDS
TAGAAGGGAATCCTTGAAGA+TGG 0.574172 3:+92502151 MsG0380017140.01.T01:CDS
AGATGAAGGTGGATCATCAC+CGG 0.581928 3:+92502421 MsG0380017140.01.T01:CDS
AAGCTTACTGTGCCAGTACA+CGG 0.584290 3:+92502629 MsG0380017140.01.T01:CDS
AGCTGCTTGAGCAAGCAAGT+TGG 0.584333 3:-92501768 None:intergenic
GCTGCAACTAACAGCTGAAG+AGG 0.588648 3:+92501736 MsG0380017140.01.T01:CDS
GAGTCACTCAGATCTCCAAA+AGG 0.591878 3:-92502589 None:intergenic
GGCAGAAGCAGAACAAGCCA+AGG 0.593446 3:+92501814 MsG0380017140.01.T01:CDS
TTAACCAGAATAAAAGCCCC+AGG 0.593700 3:+92502291 MsG0380017140.01.T01:CDS
GGAATCCTTGAAGATGGCAA+TGG 0.598116 3:+92502157 MsG0380017140.01.T01:CDS
ACTCAGATCTCCAAAAGGAT+GGG 0.602523 3:-92502584 None:intergenic
AAGGCTGGTGAGTCTGGCCA+AGG 0.604901 3:+92502263 MsG0380017140.01.T01:CDS
TGGTGAGTCTGGCCAAGGAG+TGG 0.623365 3:+92502268 MsG0380017140.01.T01:CDS
CAGATCTCCAAAAGGATGGG+AGG 0.641222 3:-92502581 None:intergenic
TAGAACAAGAACTTAGAAGG+TGG 0.641248 3:+92502216 MsG0380017140.01.T01:CDS
AAAGCTGAGAAGGCAAAGGA+AGG 0.644947 3:+92502182 MsG0380017140.01.T01:CDS
GGAGAGCTGAAAATGAGCAA+CGG 0.649631 3:+92502237 MsG0380017140.01.T01:CDS
TCATCACCGGAATCAAAACA+TGG 0.652989 3:+92502434 MsG0380017140.01.T01:CDS
TAACCAGAATAAAAGCCCCA+GGG 0.691189 3:+92502292 MsG0380017140.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATGCACATAATTTCAATTT+GGG - Chr3:92502541-92502560 None:intergenic 20.0%
! AAATAGAAGCAAAAATGAAG+TGG + Chr3:92501950-92501969 MsG0380017140.01.T01:intron 25.0%
! CAATGCACATAATTTCAATT+TGG - Chr3:92502542-92502561 None:intergenic 25.0%
!!! TTTTTTCCATGTTTTGATTC+CGG - Chr3:92502443-92502462 None:intergenic 25.0%
AAAACACATTCAAACAAGTC+AGG + Chr3:92502510-92502529 MsG0380017140.01.T01:intron 30.0%
AAACACATTCAAACAAGTCA+GGG + Chr3:92502511-92502530 MsG0380017140.01.T01:intron 30.0%
!! AGGAAGCAAATAATTTTGAC+CGG + Chr3:92502333-92502352 MsG0380017140.01.T01:CDS 30.0%
AAGGCAAAGGAAGGTAAATT+AGG + Chr3:92502191-92502210 MsG0380017140.01.T01:CDS 35.0%
ATGCATGCAGAAAAAGATGA+AGG + Chr3:92502407-92502426 MsG0380017140.01.T01:CDS 35.0%
GTGTAGAACAAGAACTTAGA+AGG + Chr3:92502213-92502232 MsG0380017140.01.T01:CDS 35.0%
TAGAACAAGAACTTAGAAGG+TGG + Chr3:92502216-92502235 MsG0380017140.01.T01:CDS 35.0%
TCTGCATGCATATAAGTCTT+CGG - Chr3:92502398-92502417 None:intergenic 35.0%
TGAAGAACTGCAGAAAGTAA+AGG + Chr3:92501793-92501812 MsG0380017140.01.T01:CDS 35.0%
! TTTTTCCATTGCCATCTTCA+AGG - Chr3:92502165-92502184 None:intergenic 35.0%
AAGAAGAATCGTTGTTCCCA+CGG + Chr3:92502464-92502483 MsG0380017140.01.T01:CDS 40.0%
ACTCAGATCTCCAAAAGGAT+GGG - Chr3:92502587-92502606 None:intergenic 40.0%
ACTGAAATCCTTCGAGAAGT+TGG + Chr3:92502103-92502122 MsG0380017140.01.T01:CDS 40.0%
ATAGAGAGATAGCTGCTAGA+AGG + Chr3:92502135-92502154 MsG0380017140.01.T01:CDS 40.0%
CAAAGAACATCATAACCCGT+GGG - Chr3:92502483-92502502 None:intergenic 40.0%
CATGCAGAAAAAGATGAAGG+TGG + Chr3:92502410-92502429 MsG0380017140.01.T01:CDS 40.0%
CCAGTGGTTTACACTTTCTT+TGG + Chr3:92501979-92501998 MsG0380017140.01.T01:intron 40.0%
GGACTTGACAAATATTGTGC+AGG - Chr3:92502377-92502396 None:intergenic 40.0%
TAACCAGAATAAAAGCCCCA+GGG + Chr3:92502292-92502311 MsG0380017140.01.T01:CDS 40.0%
TAGAAGGGAATCCTTGAAGA+TGG + Chr3:92502151-92502170 MsG0380017140.01.T01:CDS 40.0%
TAGAGAGATAGCTGCTAGAA+GGG + Chr3:92502136-92502155 MsG0380017140.01.T01:CDS 40.0%
TAGCAGCTGCAATCTTGTAT+TGG - Chr3:92502053-92502072 None:intergenic 40.0%
TCATCACCGGAATCAAAACA+TGG + Chr3:92502434-92502453 MsG0380017140.01.T01:CDS 40.0%
TGAAAATGAGCAACGGAGAA+AGG + Chr3:92502244-92502263 MsG0380017140.01.T01:CDS 40.0%
TTAACCAGAATAAAAGCCCC+AGG + Chr3:92502291-92502310 MsG0380017140.01.T01:CDS 40.0%
! AGAAAGTGTAAACCACTGGA+CGG - Chr3:92501978-92501997 None:intergenic 40.0%
! CCAAAGAAAGTGTAAACCAC+TGG - Chr3:92501982-92502001 None:intergenic 40.0%
! TATTCTGGTTAACCACTCCT+TGG - Chr3:92502283-92502302 None:intergenic 40.0%
! TGCAAAGGCTTCTGAAAAGT+TGG + Chr3:92501890-92501909 MsG0380017140.01.T01:intron 40.0%
! TTGCAGCTTCTTTGGAAGTT+CGG - Chr3:92501723-92501742 None:intergenic 40.0%
!! AGCTGCTAATTCTGAAGTTG+AGG + Chr3:92502065-92502084 MsG0380017140.01.T01:intron 40.0%
!! TGCAGGATTAGTTGCATACT+GGG - Chr3:92502360-92502379 None:intergenic 40.0%
AAAGCTGAGAAGGCAAAGGA+AGG + Chr3:92502182-92502201 MsG0380017140.01.T01:CDS 45.0%
AAAGGAGATAGAGGCTGCAA+AGG + Chr3:92501875-92501894 MsG0380017140.01.T01:intron 45.0%
AACAGCTGAAGAGGCTAATC+AGG + Chr3:92501745-92501764 MsG0380017140.01.T01:CDS 45.0%
AAGCTTACTGTGCCAGTACA+CGG + Chr3:92502629-92502648 MsG0380017140.01.T01:CDS 45.0%
AGAAGAATCGTTGTTCCCAC+GGG + Chr3:92502465-92502484 MsG0380017140.01.T01:CDS 45.0%
AGATGAAGGTGGATCATCAC+CGG + Chr3:92502421-92502440 MsG0380017140.01.T01:CDS 45.0%
AGCAGTTACTTGCAGCTCAA+AGG + Chr3:92501857-92501876 MsG0380017140.01.T01:intron 45.0%
CACTCAGATCTCCAAAAGGA+TGG - Chr3:92502588-92502607 None:intergenic 45.0%
CACTTCATCCAACTTCTCGA+AGG - Chr3:92502114-92502133 None:intergenic 45.0%
CTGCAAGTAACTGCTTTCCA+AGG - Chr3:92501852-92501871 None:intergenic 45.0%
GAGTCACTCAGATCTCCAAA+AGG - Chr3:92502592-92502611 None:intergenic 45.0%
GATCTGAGTGACTCGAATGT+TGG + Chr3:92502598-92502617 MsG0380017140.01.T01:CDS 45.0%
GCAAAGAACATCATAACCCG+TGG - Chr3:92502484-92502503 None:intergenic 45.0%
GCTGTTAGTTGCAGCTTCTT+TGG - Chr3:92501731-92501750 None:intergenic 45.0%
GGAAAAAGCTGAGAAGGCAA+AGG + Chr3:92502178-92502197 MsG0380017140.01.T01:CDS 45.0%
GGAATCCTTGAAGATGGCAA+TGG + Chr3:92502157-92502176 MsG0380017140.01.T01:CDS 45.0%
GGAGAGCTGAAAATGAGCAA+CGG + Chr3:92502237-92502256 MsG0380017140.01.T01:CDS 45.0%
GGCAATGGAAAAAGCTGAGA+AGG + Chr3:92502172-92502191 MsG0380017140.01.T01:CDS 45.0%
TTGCAGCTCAAAGGAGATAG+AGG + Chr3:92501866-92501885 MsG0380017140.01.T01:intron 45.0%
! GAAAGTGTAAACCACTGGAC+GGG - Chr3:92501977-92501996 None:intergenic 45.0%
! GATTAGTTGCATACTGGGAC+CGG - Chr3:92502355-92502374 None:intergenic 45.0%
! TATCTTGCCTCCCATCCTTT+TGG + Chr3:92502574-92502593 MsG0380017140.01.T01:CDS 45.0%
!! GTACTGGCACAGTAAGCTTT+TGG - Chr3:92502628-92502647 None:intergenic 45.0%
!! GTGCAGGATTAGTTGCATAC+TGG - Chr3:92502361-92502380 None:intergenic 45.0%
AATGAGCAACGGAGAAAGGC+TGG + Chr3:92502248-92502267 MsG0380017140.01.T01:CDS 50.0%
AGCTGCTTGAGCAAGCAAGT+TGG - Chr3:92501771-92501790 None:intergenic 50.0%
CAAGGCTGGAGTAAGTACCT+TGG + Chr3:92501832-92501851 MsG0380017140.01.T01:intron 50.0%
CAGATCTCCAAAAGGATGGG+AGG - Chr3:92502584-92502603 None:intergenic 50.0%
GCTGCAACTAACAGCTGAAG+AGG + Chr3:92501736-92501755 MsG0380017140.01.T01:CDS 50.0%
! CAAGGTACTTACTCCAGCCT+TGG - Chr3:92501834-92501853 None:intergenic 50.0%
! TAGCCCTGGGGCTTTTATTC+TGG - Chr3:92502298-92502317 None:intergenic 50.0%
AATGAAGTGGACCCGTCCAG+TGG + Chr3:92501963-92501982 MsG0380017140.01.T01:intron 55.0%
GAAGCAGAACAAGCCAAGGC+TGG + Chr3:92501818-92501837 MsG0380017140.01.T01:CDS 55.0%
GCTTAGCAAACGAGCTCACG+AGG + Chr3:92502015-92502034 MsG0380017140.01.T01:intron 55.0%
GGCAGAAGCAGAACAAGCCA+AGG + Chr3:92501814-92501833 MsG0380017140.01.T01:CDS 55.0%
!!! AAGCCCCAGGGCTAGTTTTG+AGG + Chr3:92502304-92502323 MsG0380017140.01.T01:CDS 55.0%
!!! AGCCCCAGGGCTAGTTTTGA+GGG + Chr3:92502305-92502324 MsG0380017140.01.T01:CDS 55.0%
!!! GGCTAGTTTTGAGGGGGGTA+AGG + Chr3:92502313-92502332 MsG0380017140.01.T01:CDS 55.0%
AAGGCTGGTGAGTCTGGCCA+AGG + Chr3:92502263-92502282 MsG0380017140.01.T01:CDS 60.0%
ACCCCCCTCAAAACTAGCCC+TGG - Chr3:92502312-92502331 None:intergenic 60.0%
CCCCCCTCAAAACTAGCCCT+GGG - Chr3:92502311-92502330 None:intergenic 60.0%
CCCCCTCAAAACTAGCCCTG+GGG - Chr3:92502310-92502329 None:intergenic 60.0%
CGGAGAAAGGCTGGTGAGTC+TGG + Chr3:92502257-92502276 MsG0380017140.01.T01:CDS 60.0%
TGGTGAGTCTGGCCAAGGAG+TGG + Chr3:92502268-92502287 MsG0380017140.01.T01:CDS 60.0%
!!! CCCAGGGCTAGTTTTGAGGG+GGG + Chr3:92502308-92502327 MsG0380017140.01.T01:CDS 60.0%
!!! CCCCAGGGCTAGTTTTGAGG+GGG + Chr3:92502307-92502326 MsG0380017140.01.T01:CDS 60.0%
!!! GCCCCAGGGCTAGTTTTGAG+GGG + Chr3:92502306-92502325 MsG0380017140.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr3 gene 92501716 92502654 92501716 ID=MsG0380017140.01;Name=MsG0380017140.01
Chr3 mRNA 92501716 92502654 92501716 ID=MsG0380017140.01.T01;Parent=MsG0380017140.01;Name=MsG0380017140.01.T01;_AED=0.37;_eAED=0.73;_QI=0|0|0|1|1|1|5|0|198
Chr3 exon 92501716 92501841 92501716 ID=MsG0380017140.01.T01:exon:12585;Parent=MsG0380017140.01.T01
Chr3 exon 92502098 92502486 92502098 ID=MsG0380017140.01.T01:exon:12586;Parent=MsG0380017140.01.T01
Chr3 exon 92502573 92502654 92502573 ID=MsG0380017140.01.T01:exon:12587;Parent=MsG0380017140.01.T01
Chr3 CDS 92501716 92501841 92501716 ID=MsG0380017140.01.T01:cds;Parent=MsG0380017140.01.T01
Chr3 CDS 92502098 92502486 92502098 ID=MsG0380017140.01.T01:cds;Parent=MsG0380017140.01.T01
Chr3 CDS 92502573 92502654 92502573 ID=MsG0380017140.01.T01:cds;Parent=MsG0380017140.01.T01
Gene Sequence

>MsG0380017140.01.T01

ATGACCGAACTTCCAAAGAAGCTGCAACTAACAGCTGAAGAGGCTAATCAGGCCAACTTGCTTGCTCAAGCAGCTCGTGAAGAACTGCAGAAAGTAAAGGCAGAAGCAGAACAAGCCAAGGCTGGATCTGAACTGAAATCCTTCGAGAAGTTGGATGAAGTGAATAGAGAGATAGCTGCTAGAAGGGAATCCTTGAAGATGGCAATGGAAAAAGCTGAGAAGGCAAAGGAAGGTAAATTAGGTGTAGAACAAGAACTTAGAAGGTGGAGAGCTGAAAATGAGCAACGGAGAAAGGCTGGTGAGTCTGGCCAAGGAGTGGTTAACCAGAATAAAAGCCCCAGGGCTAGTTTTGAGGGGGGTAAGGAAGCAAATAATTTTGACCGGTCCCAGTATGCAACTAATCCTGCACAATATTTGTCAAGTCCGAAGACTTATATGCATGCAGAAAAAGATGAAGGTGGATCATCACCGGAATCAAAACATGGAAAAAAGAAGAAGAATCGTTGTTCCCACGGTTATCTTGCCTCCCATCCTTTTGGAGATCTGAGTGACTCGAATGTTGGATTACCAAAAGCTTACTGTGCCAGTACACGGTGA

Protein sequence

>MsG0380017140.01.T01

MTELPKKLQLTAEEANQANLLAQAAREELQKVKAEAEQAKAGSELKSFEKLDEVNREIAARRESLKMAMEKAEKAKEGKLGVEQELRRWRAENEQRRKAGESGQGVVNQNKSPRASFEGGKEANNFDRSQYATNPAQYLSSPKTYMHAEKDEGGSSPESKHGKKKKNRCSHGYLASHPFGDLSDSNVGLPKAYCASTR*