AlfalfaGEDB | MsG0880047742.01

M. sativa cultivar ZhongmuNo.1 / MsG0880047742.01

KEGG: K08065
GO: GO:0005634 GO:0006355 GO:0043565 GO:0046982
PFAM: PF00808

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880047742.01.T01	XP_013458655.1	96.324	136	5	0	1	136	1	136	1.46E-90	268

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880047742.01.T01	O23310	75.424	118	23	2	1	114	1	116	2.85E-59	183

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880047742.01.T01	I3TAW6	96.324	136	5	0	1	136	1	136	6.97e-91	268

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180000284.01	MsG0880047742.01	0.833519	5.089810e-56	5.876078e-53
MsG0180000293.01	MsG0880047742.01	0.805831	1.103701e-49	5.898635e-47
MsG0880044075.01	MsG0880047742.01	0.810843	9.423851e-51	5.742430e-48
MsG0880045106.01	MsG0880047742.01	0.829043	6.418066e-55	6.492321e-52
MsG0880046047.01	MsG0880047742.01	0.827400	1.597882e-54	1.541058e-51
MsG0880046113.01	MsG0880047742.01	0.802050	6.743894e-49	3.270967e-46
MsG0880046244.01	MsG0880047742.01	0.806901	6.568994e-50	3.608057e-47
MsG0880046317.01	MsG0880047742.01	0.835065	2.084846e-56	2.520823e-53
MsG0880046329.01	MsG0880047742.01	0.818148	2.280357e-52	1.693285e-49
MsG0880046532.01	MsG0880047742.01	0.814531	1.469264e-51	9.883684e-49
MsG0880046642.01	MsG0880047742.01	0.803045	4.204207e-49	2.091442e-46
MsG0880046733.01	MsG0880047742.01	0.804647	1.953546e-49	1.012721e-46
MsG0880047191.01	MsG0880047742.01	0.839988	1.139983e-57	1.601487e-54
MsG0880047391.01	MsG0880047742.01	0.814239	1.704687e-51	1.137708e-48
MsG0880047590.01	MsG0880047742.01	0.850484	1.648458e-60	3.233591e-57
MsG0180003190.01	MsG0880047742.01	0.810506	1.114090e-50	6.727736e-48
MsG0180003669.01	MsG0880047742.01	0.809731	1.637120e-50	9.687424e-48
MsG0180003948.01	MsG0880047742.01	0.815676	8.180683e-52	5.677362e-49
MsG0180004157.01	MsG0880047742.01	0.818872	1.562184e-52	1.183504e-49
MsG0180004853.01	MsG0880047742.01	0.800134	1.661987e-48	7.679189e-46
MsG0180004984.01	MsG0880047742.01	0.841301	5.162401e-58	7.552487e-55
MsG0180005125.01	MsG0880047742.01	0.824688	7.047748e-54	6.288592e-51
MsG0180005398.01	MsG0880047742.01	0.817615	3.008574e-52	2.201579e-49
MsG0180005813.01	MsG0880047742.01	0.820629	6.201887e-53	4.932738e-50
MsG0180005842.01	MsG0880047742.01	0.823008	1.745062e-53	1.484494e-50
MsG0180005949.01	MsG0880047742.01	0.807252	5.534477e-50	3.067786e-47
MsG0180006016.01	MsG0880047742.01	0.835911	1.273962e-56	1.580085e-53
MsG0280006356.01	MsG0880047742.01	0.839814	1.264730e-57	1.766936e-54
MsG0780040344.01	MsG0880047742.01	0.821469	3.970958e-53	3.233292e-50
MsG0780040442.01	MsG0880047742.01	0.822527	2.258517e-53	1.895279e-50
MsG0780040516.01	MsG0880047742.01	0.800712	1.267473e-48	5.942542e-46
MsG0780041065.01	MsG0880047742.01	0.813891	2.034762e-51	1.345210e-48
MsG0780041161.01	MsG0880047742.01	0.809749	1.622046e-50	9.603160e-48
MsG0780041220.01	MsG0880047742.01	0.820792	5.687579e-53	4.544750e-50
MsG0780041255.01	MsG0880047742.01	0.812011	5.255307e-51	3.302960e-48
MsG0780041376.01	MsG0880047742.01	0.824885	6.335142e-54	5.684559e-51
MsG0780041754.01	MsG0880047742.01	0.822158	2.750530e-53	2.283814e-50
MsG0880041969.01	MsG0880047742.01	0.833799	4.333241e-56	5.044905e-53
MsG0880042151.01	MsG0880047742.01	0.801879	7.311486e-49	3.531060e-46
MsG0880042811.01	MsG0880047742.01	0.835389	1.726834e-56	2.108617e-53
MsG0880042843.01	MsG0880047742.01	0.821159	4.681909e-53	3.779648e-50
MsG0880043001.01	MsG0880047742.01	0.803686	3.096206e-49	1.565870e-46
MsG0880043082.01	MsG0880047742.01	0.818615	1.787210e-52	1.344269e-49
MsG0880043591.01	MsG0880047742.01	0.805115	1.560256e-49	8.186284e-47
MsG0880043804.01	MsG0880047742.01	0.805005	1.645124e-49	8.607095e-47
MsG0280010614.01	MsG0880047742.01	0.816106	6.562547e-52	4.608123e-49
MsG0280010784.01	MsG0880047742.01	0.805633	1.214783e-49	6.459272e-47
MsG0280011070.01	MsG0880047742.01	0.802731	4.882323e-49	2.409584e-46
MsG0280011235.01	MsG0880047742.01	0.816903	4.349531e-52	3.121502e-49
MsG0280011446.01	MsG0880047742.01	0.809925	1.486573e-50	8.841479e-48
MsG0380011729.01	MsG0880047742.01	0.821611	3.683498e-53	3.011167e-50
MsG0380011754.01	MsG0880047742.01	0.810746	9.888522e-51	6.010019e-48
MsG0380012020.01	MsG0880047742.01	0.813757	2.177791e-51	1.434523e-48
MsG0380012124.01	MsG0880047742.01	0.828851	7.142258e-55	7.184334e-52
MsG0380012930.01	MsG0880047742.01	0.808878	2.494706e-50	1.442888e-47
MsG0380014365.01	MsG0880047742.01	0.846557	2.015448e-59	3.482443e-56
MsG0380014573.01	MsG0880047742.01	0.816700	4.831391e-52	3.447938e-49
MsG0680030841.01	MsG0880047742.01	0.804850	1.772304e-49	9.236280e-47
MsG0680031651.01	MsG0880047742.01	0.807677	4.498070e-50	2.520912e-47
MsG0680034450.01	MsG0880047742.01	0.821965	3.048988e-53	2.517702e-50
MsG0680035048.01	MsG0880047742.01	0.800675	1.289455e-48	6.040365e-46
MsG0680035656.01	MsG0880047742.01	0.807595	4.682074e-50	2.618316e-47
MsG0480020065.01	MsG0880047742.01	0.814700	1.348379e-51	9.111589e-49
MsG0480020090.01	MsG0880047742.01	0.844114	9.238930e-59	1.476610e-55
MsG0480020372.01	MsG0880047742.01	0.803077	4.141066e-49	2.061663e-46
MsG0480020416.01	MsG0880047742.01	0.835190	1.938602e-56	2.353046e-53
MsG0480020445.01	MsG0880047742.01	0.826009	3.432184e-54	3.179809e-51
MsG0480020791.01	MsG0880047742.01	0.828384	9.265844e-55	9.195689e-52
MsG0480021240.01	MsG0880047742.01	0.801653	8.135070e-49	3.906037e-46
MsG0480021298.01	MsG0880047742.01	0.803051	4.192772e-49	2.086045e-46
MsG0480021464.01	MsG0880047742.01	0.818034	2.419523e-52	1.790992e-49
MsG0480021695.01	MsG0880047742.01	0.836809	7.525540e-57	9.590424e-54
MsG0480022104.01	MsG0880047742.01	0.800675	1.289614e-48	6.041081e-46
MsG0480022183.01	MsG0880047742.01	0.807080	6.017891e-50	3.320756e-47
MsG0480022752.01	MsG0880047742.01	0.819615	1.058257e-52	8.183492e-50
MsG0480023014.01	MsG0880047742.01	0.835124	2.013753e-56	2.439253e-53
MsG0480023025.01	MsG0880047742.01	0.806590	7.641165e-50	4.163437e-47
MsG0480023088.01	MsG0880047742.01	0.823440	1.383254e-53	1.191060e-50
MsG0480023224.01	MsG0880047742.01	0.857493	1.572477e-62	3.888198e-59
MsG0480023245.01	MsG0880047742.01	0.810910	9.112641e-51	5.562935e-48
MsG0480023517.01	MsG0880047742.01	0.811409	7.101868e-51	4.392918e-48
MsG0480023718.01	MsG0880047742.01	0.800358	1.496134e-48	6.952488e-46
MsG0580024405.01	MsG0880047742.01	0.812196	4.788697e-51	3.025018e-48
MsG0580024583.01	MsG0880047742.01	0.812379	4.366198e-51	2.771773e-48
MsG0580025489.01	MsG0880047742.01	0.809337	1.989085e-50	1.164725e-47
MsG0580025749.01	MsG0880047742.01	0.816676	4.890401e-52	3.487832e-49
MsG0580025758.01	MsG0880047742.01	0.804431	2.167182e-49	1.117179e-46
MsG0580025989.01	MsG0880047742.01	0.824834	6.512319e-54	5.834892e-51
MsG0380015564.01	MsG0880047742.01	0.801389	9.216355e-49	4.395852e-46
MsG0380016055.01	MsG0880047742.01	0.828372	9.324929e-55	9.250990e-52
MsG0380016244.01	MsG0880047742.01	0.821272	4.410364e-53	3.571392e-50
MsG0380016348.01	MsG0880047742.01	0.815912	7.247889e-52	5.062176e-49
MsG0380016581.01	MsG0880047742.01	0.837102	6.335345e-57	8.145054e-54
MsG0380016624.01	MsG0880047742.01	0.823166	1.602919e-53	1.369647e-50
MsG0380017140.01	MsG0880047742.01	0.837801	4.195128e-57	5.511070e-54
MsG0380017158.01	MsG0880047742.01	0.822173	2.728942e-53	2.266788e-50
MsG0380017226.01	MsG0880047742.01	0.825769	3.912072e-54	3.599745e-51
MsG0380017403.01	MsG0880047742.01	0.842021	3.335063e-58	4.990486e-55
MsG0380018009.01	MsG0880047742.01	0.802773	4.785095e-49	2.364181e-46
MsG0480018160.01	MsG0880047742.01	0.832983	6.923209e-56	7.865484e-53
MsG0480018295.01	MsG0880047742.01	0.802261	6.102383e-49	2.975782e-46
MsG0580027074.01	MsG0880047742.01	0.823330	1.468034e-53	1.260116e-50
MsG0580027129.01	MsG0880047742.01	0.830075	3.601487e-55	3.754595e-52
MsG0580027152.01	MsG0880047742.01	0.800422	1.451875e-48	6.757865e-46
MsG0580027228.01	MsG0880047742.01	0.850848	1.302455e-60	2.585957e-57
MsG0580027345.01	MsG0880047742.01	0.829165	5.994662e-55	6.086734e-52
MsG0580028185.01	MsG0880047742.01	0.800857	1.183665e-48	5.570615e-46
MsG0580028284.01	MsG0880047742.01	0.835720	1.423889e-56	1.756013e-53
MsG0580028289.01	MsG0880047742.01	0.841099	5.834070e-58	8.481482e-55
MsG0580028605.01	MsG0880047742.01	0.804941	1.696245e-49	8.860775e-47
MsG0580029658.01	MsG0880047742.01	0.801260	9.791447e-49	4.655126e-46
MsG0680030316.01	MsG0880047742.01	0.812315	4.510653e-51	2.858546e-48
MsG0680030463.01	MsG0880047742.01	0.816929	4.292741e-52	3.082956e-49
MsG0680030656.01	MsG0880047742.01	0.814232	1.710491e-51	1.141343e-48
MsG0280006728.01	MsG0880047742.01	0.800959	1.128311e-48	5.323874e-46
MsG0280007549.01	MsG0880047742.01	0.816463	5.459941e-52	3.871231e-49
MsG0280007648.01	MsG0880047742.01	0.824326	8.576791e-54	7.572250e-51
MsG0280007815.01	MsG0880047742.01	0.817531	3.143001e-52	2.294678e-49
MsG0280008039.01	MsG0880047742.01	0.811474	6.874439e-51	4.259557e-48
MsG0280008286.01	MsG0880047742.01	0.852709	3.863435e-61	8.143536e-58
MsG0280008417.01	MsG0880047742.01	0.800131	1.664462e-48	7.690034e-46
MsG0280008556.01	MsG0880047742.01	0.832825	7.579539e-56	8.569768e-53
MsG0280008615.01	MsG0880047742.01	0.826437	2.713518e-54	2.544819e-51
MsG0280008907.01	MsG0880047742.01	0.824969	6.050483e-54	5.442035e-51
MsG0280008970.01	MsG0880047742.01	0.838363	3.006018e-57	4.016994e-54
MsG0280008971.01	MsG0880047742.01	0.808024	3.794027e-50	2.145827e-47
MsG0280009024.01	MsG0880047742.01	0.838464	2.830910e-57	3.794833e-54
MsG0280010389.01	MsG0880047742.01	0.848501	5.888914e-60	1.083025e-56
MsG0280010416.01	MsG0880047742.01	0.816086	6.629394e-52	4.652635e-49
MsG0280010459.01	MsG0880047742.01	0.805683	1.185654e-49	6.312685e-47
MsG0680035777.01	MsG0880047742.01	0.806895	6.587315e-50	3.617547e-47
MsG0780037142.01	MsG0880047742.01	0.809468	1.864478e-50	1.095548e-47
MsG0780037287.01	MsG0880047742.01	0.807953	3.928082e-50	2.217469e-47
MsG0780038921.01	MsG0880047742.01	0.822953	1.797150e-53	1.526391e-50
MsG0780039452.01	MsG0880047742.01	0.809396	1.931432e-50	1.132783e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880047742.01.T01	MTR_4g133938	96.324	136	5	0	1	136	1	136	1.77e-94	268
MsG0880047742.01.T01	MTR_2g026710	75.385	130	27	2	1	130	1	125	1.25e-64	194
MsG0880047742.01.T01	MTR_5g095740	75.000	120	26	2	1	116	1	120	1.54e-59	182
MsG0880047742.01.T01	MTR_3g058980	71.901	121	26	1	1	113	1	121	4.46e-59	181
MsG0880047742.01.T01	MTR_1g072790	70.588	119	32	2	10	127	22	138	7.09e-55	170
MsG0880047742.01.T01	MTR_8g091720	81.443	97	18	0	20	116	29	125	1.05e-54	170
MsG0880047742.01.T01	MTR_8g093920	79.787	94	19	0	20	113	41	134	2.95e-54	169
MsG0880047742.01.T01	MTR_0392s0020	68.182	110	33	1	6	113	3	112	6.58e-53	164
MsG0880047742.01.T01	MTR_4g119500	68.182	110	30	1	4	113	12	116	7.68e-53	163
MsG0880047742.01.T01	MTR_7g061270	65.079	126	41	1	7	129	14	139	2.10e-52	163
MsG0880047742.01.T01	MTR_7g100650	65.079	126	41	1	7	129	14	139	3.55e-52	163
MsG0880047742.01.T01	MTR_1g088860	74.468	94	24	0	20	113	37	130	1.38e-51	162
MsG0880047742.01.T01	MTR_4g133952	67.021	94	31	0	20	113	59	152	1.68e-45	148
MsG0880047742.01.T01	MTR_1g083070	54.867	113	45	1	21	133	6	112	4.36e-42	135
MsG0880047742.01.T01	MTR_1g039040	57.426	101	41	1	13	113	2	100	7.13e-40	132
MsG0880047742.01.T01	MTR_1g029070	53.261	92	43	0	20	111	53	144	1.47e-28	103
MsG0880047742.01.T01	MTR_5g095900	46.667	105	51	2	7	108	3	105	4.92e-25	94.4
MsG0880047742.01.T01	MTR_1g028480	51.515	66	32	0	49	114	19	84	6.43e-19	76.6
MsG0880047742.01.T01	MTR_1g029100	40.789	76	45	0	24	99	7	82	6.88e-19	75.9
MsG0880047742.01.T01	MTR_2g056000	30.952	84	58	0	24	107	46	129	2.30e-13	63.9
MsG0880047742.01.T01	MTR_4g112380	30.952	84	58	0	24	107	16	99	4.02e-13	62.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880047742.01.T01	AT4G14540	75.424	118	23	2	1	114	1	116	2.91e-60	183
MsG0880047742.01.T01	AT5G47640	82.105	95	17	0	20	114	28	122	4.78e-55	171
MsG0880047742.01.T01	AT2G38880	65.323	124	39	3	9	130	12	133	4.32e-52	162
MsG0880047742.01.T01	AT2G47810	76.596	94	22	0	20	113	52	145	4.88e-52	162
MsG0880047742.01.T01	AT2G38880	65.323	124	39	3	9	130	12	133	6.33e-52	161
MsG0880047742.01.T01	AT2G38880	65.323	124	39	3	9	130	12	133	6.33e-52	161
MsG0880047742.01.T01	AT2G38880	65.323	124	39	3	9	130	12	133	6.33e-52	161
MsG0880047742.01.T01	AT2G13570	76.596	94	22	0	20	113	37	130	1.94e-51	162
MsG0880047742.01.T01	AT3G53340	74.194	93	24	0	20	112	30	122	3.01e-50	158
MsG0880047742.01.T01	AT3G53340	74.194	93	24	0	20	112	30	122	3.01e-50	158
MsG0880047742.01.T01	AT2G37060	69.725	109	29	2	7	112	16	123	3.65e-50	158
MsG0880047742.01.T01	AT2G37060	69.725	109	29	2	7	112	16	123	3.65e-50	158
MsG0880047742.01.T01	AT2G37060	69.725	109	29	2	7	112	16	123	3.65e-50	158
MsG0880047742.01.T01	AT2G38880	72.549	102	25	2	9	109	12	111	1.19e-49	154
MsG0880047742.01.T01	AT2G38880	72.549	102	25	2	9	109	12	111	2.20e-49	155
MsG0880047742.01.T01	AT2G38880	72.549	102	25	2	9	109	12	111	2.20e-49	155
MsG0880047742.01.T01	AT2G38880	72.549	102	25	2	9	109	12	111	2.20e-49	155
MsG0880047742.01.T01	AT2G38880	63.415	123	41	3	9	130	12	131	3.50e-49	154
MsG0880047742.01.T01	AT2G38880	72.549	102	25	2	9	109	12	111	4.74e-49	155
MsG0880047742.01.T01	AT2G38880	72.549	102	25	2	9	109	12	111	4.74e-49	155
MsG0880047742.01.T01	AT3G53340	73.333	90	24	0	20	109	30	119	1.07e-48	152
MsG0880047742.01.T01	AT3G53340	73.626	91	24	0	20	110	30	120	3.29e-48	152
MsG0880047742.01.T01	AT3G53340	73.626	91	24	0	20	110	30	120	3.29e-48	152
MsG0880047742.01.T01	AT5G47670	63.918	97	35	0	20	116	30	126	6.46e-45	145
MsG0880047742.01.T01	AT5G47670	63.918	97	35	0	20	116	59	155	1.43e-44	145
MsG0880047742.01.T01	AT5G47670	63.918	97	35	0	20	116	59	155	1.43e-44	145
MsG0880047742.01.T01	AT1G09030	57.391	115	46	1	20	131	4	118	2.17e-44	142
MsG0880047742.01.T01	AT1G21970	58.163	98	41	0	20	117	60	157	2.91e-41	137
MsG0880047742.01.T01	AT5G08190	35.052	97	61	1	24	118	16	112	7.52e-18	75.5
MsG0880047742.01.T01	AT5G08190	34.694	98	61	2	24	118	16	113	4.21e-16	70.9
MsG0880047742.01.T01	AT5G23090	30.928	97	65	1	24	118	16	112	8.94e-15	67.4
MsG0880047742.01.T01	AT5G23090	34.118	85	54	1	24	106	16	100	5.72e-14	65.1
MsG0880047742.01.T01	AT5G23090	34.118	85	54	1	24	106	16	100	5.72e-14	65.1
MsG0880047742.01.T01	AT5G23090	34.118	85	54	1	24	106	16	100	5.72e-14	65.1
MsG0880047742.01.T01	AT5G23090	32.941	85	54	2	25	106	3	87	8.08e-11	56.6

Find 30 sgRNAs with CRISPR-Local

Find 30 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GAATCAGGTGGTCAAGCTTC+AGG	0.278269	8:+90283640	MsG0880047742.01.T01:CDS
GGGTTACCTTCACAAGTATA+GGG	0.382046	8:+90283921	MsG0880047742.01.T01:CDS
GATCATGAAGAAAGCTTTAC+CGG	0.392387	8:+90283714	MsG0880047742.01.T01:CDS
AGAATATGCAGAGCCTCTTA+AGG	0.393028	8:+90283900	MsG0880047742.01.T01:CDS
AGGGTTACCTTCACAAGTAT+AGG	0.429699	8:+90283920	MsG0880047742.01.T01:CDS
TTCGATATCTTTGCTCTCGC+CGG	0.458504	8:-90283733	None:intergenic
GAGTTCATAAGCTTCATAAC+AGG	0.460674	8:+90283790	MsG0880047742.01.T01:CDS
GATCCTACCAACGTTTGCTA+TGG	0.475579	8:-90283695	None:intergenic
GCAGAATCAGATGATGAATC+AGG	0.475967	8:+90283625	MsG0880047742.01.T01:CDS
GAGAAGAGGAAGACAATAAA+CGG	0.477283	8:+90283838	MsG0880047742.01.T01:CDS
TTGTGAAGGTAACCCTTAAG+AGG	0.483388	8:-90283913	None:intergenic
CAATTTCCCTATACTTGTGA+AGG	0.485169	8:-90283927	None:intergenic
GAATATGCAGAGCCTCTTAA+GGG	0.488554	8:+90283901	MsG0880047742.01.T01:CDS
AATCAGGTGGTCAAGCTTCA+GGG	0.501937	8:+90283641	MsG0880047742.01.T01:CDS
GAATATGATTGGTAGTAGTA+AGG	0.514281	8:+90283972	MsG0880047742.01.T01:CDS
GCAGAGAATTACTGCAAGAA+AGG	0.517934	8:+90283665	MsG0880047742.01.T01:CDS
CACAAGTATAGGGAAATTGA+AGG	0.531636	8:+90283931	MsG0880047742.01.T01:CDS
GTTGCAAATATAATCAACCT+TGG	0.534009	8:-90284023	None:intergenic
ATCCTACCAACGTTTGCTAT+GGG	0.537868	8:-90283694	None:intergenic
AAAAGTGTCAGAAAGAGAAG+AGG	0.551950	8:+90283824	MsG0880047742.01.T01:CDS
AGTATTGATCGATTCTTCCA+AGG	0.558864	8:+90284006	MsG0880047742.01.T01:CDS
TTCAAAGCCAAGTGTAGTCA+TGG	0.563942	8:-90283878	None:intergenic
GTGTGGGCCATGACTACACT+TGG	0.565546	8:+90283871	MsG0880047742.01.T01:CDS
TACCAACGTTTGCTATGGGA+AGG	0.599059	8:-90283690	None:intergenic
TTCCCATAGCAAACGTTGGT+AGG	0.621000	8:+90283692	MsG0880047742.01.T01:CDS
CTCCTTCCCATAGCAAACGT+TGG	0.626066	8:+90283688	MsG0880047742.01.T01:CDS
GGTAGTAGTAAGGAGCAGCA+AGG	0.645799	8:+90283982	MsG0880047742.01.T01:CDS
TAAACGGTGATGATCTTGTG+TGG	0.661494	8:+90283854	MsG0880047742.01.T01:CDS
AAACGGTGATGATCTTGTGT+GGG	0.691215	8:+90283855	MsG0880047742.01.T01:CDS
GAATCAGATGATGAATCAGG+TGG	0.704177	8:+90283628	MsG0880047742.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AATTTTTCTATGAATATGAT+TGG	+	Chr8:90283961-90283980	MsG0880047742.01.T01:CDS	15.0%
!	GAATATGATTGGTAGTAGTA+AGG	+	Chr8:90283972-90283991	MsG0880047742.01.T01:CDS	30.0%
	CAATTTCCCTATACTTGTGA+AGG	-	Chr8:90283930-90283949	None:intergenic	35.0%
	CACAAGTATAGGGAAATTGA+AGG	+	Chr8:90283931-90283950	MsG0880047742.01.T01:CDS	35.0%
	GAGAAGAGGAAGACAATAAA+CGG	+	Chr8:90283838-90283857	MsG0880047742.01.T01:CDS	35.0%
	GAGTTCATAAGCTTCATAAC+AGG	+	Chr8:90283790-90283809	MsG0880047742.01.T01:CDS	35.0%
	GATCATGAAGAAAGCTTTAC+CGG	+	Chr8:90283714-90283733	MsG0880047742.01.T01:CDS	35.0%
!!	AAAAGTGTCAGAAAGAGAAG+AGG	+	Chr8:90283824-90283843	MsG0880047742.01.T01:CDS	35.0%
!!	AGTATTGATCGATTCTTCCA+AGG	+	Chr8:90284006-90284025	MsG0880047742.01.T01:CDS	35.0%
	AAACGGTGATGATCTTGTGT+GGG	+	Chr8:90283855-90283874	MsG0880047742.01.T01:CDS	40.0%
	AGAATATGCAGAGCCTCTTA+AGG	+	Chr8:90283900-90283919	MsG0880047742.01.T01:CDS	40.0%
	AGGGTTACCTTCACAAGTAT+AGG	+	Chr8:90283920-90283939	MsG0880047742.01.T01:CDS	40.0%
	GAATATGCAGAGCCTCTTAA+GGG	+	Chr8:90283901-90283920	MsG0880047742.01.T01:CDS	40.0%
	GAATCAGATGATGAATCAGG+TGG	+	Chr8:90283628-90283647	MsG0880047742.01.T01:CDS	40.0%
	GCAGAATCAGATGATGAATC+AGG	+	Chr8:90283625-90283644	MsG0880047742.01.T01:CDS	40.0%
	GCAGAGAATTACTGCAAGAA+AGG	+	Chr8:90283665-90283684	MsG0880047742.01.T01:CDS	40.0%
	GGGTTACCTTCACAAGTATA+GGG	+	Chr8:90283921-90283940	MsG0880047742.01.T01:CDS	40.0%
	TAAACGGTGATGATCTTGTG+TGG	+	Chr8:90283854-90283873	MsG0880047742.01.T01:CDS	40.0%
	TTCAAAGCCAAGTGTAGTCA+TGG	-	Chr8:90283881-90283900	None:intergenic	40.0%
	TTGTGAAGGTAACCCTTAAG+AGG	-	Chr8:90283916-90283935	None:intergenic	40.0%
!!	ATCCTACCAACGTTTGCTAT+GGG	-	Chr8:90283697-90283716	None:intergenic	40.0%
	AATCAGGTGGTCAAGCTTCA+GGG	+	Chr8:90283641-90283660	MsG0880047742.01.T01:CDS	45.0%
	TTCCCATAGCAAACGTTGGT+AGG	+	Chr8:90283692-90283711	MsG0880047742.01.T01:CDS	45.0%
	TTCGATATCTTTGCTCTCGC+CGG	-	Chr8:90283736-90283755	None:intergenic	45.0%
!	GATCCTACCAACGTTTGCTA+TGG	-	Chr8:90283698-90283717	None:intergenic	45.0%
!!	TACCAACGTTTGCTATGGGA+AGG	-	Chr8:90283693-90283712	None:intergenic	45.0%
	CTCCTTCCCATAGCAAACGT+TGG	+	Chr8:90283688-90283707	MsG0880047742.01.T01:CDS	50.0%
	GAATCAGGTGGTCAAGCTTC+AGG	+	Chr8:90283640-90283659	MsG0880047742.01.T01:CDS	50.0%
	GGTAGTAGTAAGGAGCAGCA+AGG	+	Chr8:90283982-90284001	MsG0880047742.01.T01:CDS	50.0%
	GTGTGGGCCATGACTACACT+TGG	+	Chr8:90283871-90283890	MsG0880047742.01.T01:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
Chr8	gene	90283622	90284032	90283622	ID=MsG0880047742.01;Name=MsG0880047742.01
Chr8	mRNA	90283622	90284032	90283622	ID=MsG0880047742.01.T01;Parent=MsG0880047742.01;Name=MsG0880047742.01.T01;_AED=0.19;_eAED=0.19;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|136
Chr8	exon	90283622	90284032	90283622	ID=MsG0880047742.01.T01:exon:2866;Parent=MsG0880047742.01.T01
Chr8	CDS	90283622	90284032	90283622	ID=MsG0880047742.01.T01:cds;Parent=MsG0880047742.01.T01

Gene Sequence

>MsG0880047742.01.T01

ATGGCAGAATCAGATGATGAATCAGGTGGTCAAGCTTCAGGGAGCAGAGAATTACTGCAAGAAAGGCTCCTTCCCATAGCAAACGTTGGTAGGATCATGAAGAAAGCTTTACCGGCGAGAGCAAAGATATCGAAAGAAGCTAAAGAAACGATGCAAGAGTGTGTATCAGAGTTCATAAGCTTCATAACAGGTGAAGCATCTGAAAAGTGTCAGAAAGAGAAGAGGAAGACAATAAACGGTGATGATCTTGTGTGGGCCATGACTACACTTGGCTTTGAAGAATATGCAGAGCCTCTTAAGGGTTACCTTCACAAGTATAGGGAAATTGAAGGTGACAAGAATTTTTCTATGAATATGATTGGTAGTAGTAAGGAGCAGCAAGGTAGTATTGATCGATTCTTCCAAGGTTGA

Protein sequence

>MsG0880047742.01.T01

MAESDDESGGQASGSRELLQERLLPIANVGRIMKKALPARAKISKEAKETMQECVSEFISFITGEASEKCQKEKRKTINGDDLVWAMTTLGFEEYAEPLKGYLHKYREIEGDKNFSMNMIGSSKEQQGSIDRFFQG*