AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280007698.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280007698.01.T01 MTR_2g030170 64.062 64 15 2 1 64 1 56 6.20e-16 68.6
MsG0280007698.01.T01 MTR_2g030170 60.000 65 16 2 1 64 1 56 2.15e-15 67.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 23 sgRNAs with CRISPR-Local

Find 137 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATTGAAGTGTTGTTGAGTT+TGG 0.299925 2:+19386118 None:intergenic
TGTCCATCAGCCTCGTTATC+TGG 0.370392 2:+19383908 None:intergenic
GGACAGAGTGCATGTGAATC+AGG 0.390221 2:-19384636 MsG0280007698.01.T01:five_prime_UTR
ATTGAAGTGTTGTTGAGTTT+GGG 0.418296 2:+19386119 None:intergenic
AAATTCCGTTTGCAGAATAT+AGG 0.427981 2:+19383860 None:intergenic
GCACCTCCACAGCTTGGCAC+GGG 0.466695 2:-19383796 MsG0280007698.01.T01:CDS
CGCCGGTATTGATAATGCTC+AGG 0.487682 2:-19383833 MsG0280007698.01.T01:CDS
TGTTGTTGAGTTTGGGACAG+TGG 0.506320 2:+19386126 None:intergenic
TGCAGCCTATATTCTGCAAA+CGG 0.508482 2:-19383865 MsG0280007698.01.T01:CDS
GCTTGGCACGGGGCATGCTA+TGG 0.522353 2:-19383785 MsG0280007698.01.T01:CDS
AGCACCTCCACAGCTTGGCA+CGG 0.523882 2:-19383797 MsG0280007698.01.T01:CDS
GCATGCCCCGTGCCAAGCTG+TGG 0.532400 2:+19383790 None:intergenic
TGCCCCGTGCCAAGCTGTGG+AGG 0.541599 2:+19383793 None:intergenic
AACCTGAGCATTATCAATAC+CGG 0.557780 2:+19383831 None:intergenic
CACCTCCACAGCTTGGCACG+GGG 0.573071 2:-19383795 MsG0280007698.01.T01:CDS
TGTCCAGATAACGAGGCTGA+TGG 0.582397 2:-19383911 MsG0280007698.01.T01:CDS
TATGCAGCACCTCCACAGCT+TGG 0.593812 2:-19383802 MsG0280007698.01.T01:CDS
GAATTGAAAATGATAGAAGA+AGG 0.623593 2:+19386087 None:intergenic
ATCATGTGTGGAAGAAGTCA+TGG 0.636060 2:+19384610 None:intergenic
TCTTCCACACATGATCCCAC+AGG 0.647344 2:-19384602 MsG0280007698.01.T01:intron
AATACCTGTGGGATCATGTG+TGG 0.669627 2:+19384598 None:intergenic
AAATTGTTGTCCAGATAACG+AGG 0.691918 2:-19383918 MsG0280007698.01.T01:CDS
GCAAACGGAATTTCTCACGC+CGG 0.699389 2:-19383850 MsG0280007698.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAACTAATAACATAATAA+TGG + Chr2:19385893-19385912 None:intergenic 10.0%
!! ATCTAAAAAGATTCAAAATT+TGG + Chr2:19384000-19384019 None:intergenic 15.0%
!! TAGAAAAATCTATAATTGAA+TGG - Chr2:19385197-19385216 MsG0280007698.01.T01:intron 15.0%
!! TATCAGTACTTAAATATATT+AGG + Chr2:19385533-19385552 None:intergenic 15.0%
!! TTGATAAAACAAAATCATTT+AGG - Chr2:19385430-19385449 MsG0280007698.01.T01:intron 15.0%
!!! AAAATTTAGAATTGTCATTT+TGG - Chr2:19384366-19384385 MsG0280007698.01.T01:intron 15.0%
!! AAAAACCCAAAATAGAAAAT+TGG + Chr2:19384168-19384187 None:intergenic 20.0%
!! AAAACCCAAAATAGAAAATT+GGG + Chr2:19384167-19384186 None:intergenic 20.0%
!! ATTAGTTAAAGCTTATTATG+AGG - Chr2:19384467-19384486 MsG0280007698.01.T01:intron 20.0%
!! ATTTGATTATGCTTATTAGA+TGG - Chr2:19384247-19384266 MsG0280007698.01.T01:intron 20.0%
!! CATACATTCAAATTGTATAA+GGG + Chr2:19384667-19384686 None:intergenic 20.0%
!! CATTAAATGATTGATTAACT+AGG - Chr2:19384782-19384801 MsG0280007698.01.T01:intron 20.0%
!! TGATAGAATTATTGTAAACA+GGG - Chr2:19385548-19385567 MsG0280007698.01.T01:intron 20.0%
!! TTAGAAAATTGGGTTAAAAA+GGG - Chr2:19384090-19384109 MsG0280007698.01.T01:intron 20.0%
!!! AGGAAAAAAATTGCAATTTT+TGG - Chr2:19384211-19384230 MsG0280007698.01.T01:intron 20.0%
!!! GGAAAAAAATTGCAATTTTT+GGG - Chr2:19384212-19384231 MsG0280007698.01.T01:intron 20.0%
!!! TATTGATTTTTTGGATATGA+TGG - Chr2:19384277-19384296 MsG0280007698.01.T01:intron 20.0%
! AAAACAAGTGTCAAATAGTA+TGG + Chr2:19384444-19384463 None:intergenic 25.0%
! AGAATTACTTCTTCAAATCA+TGG - Chr2:19385478-19385497 MsG0280007698.01.T01:intron 25.0%
! AGATTTCCAAAATCAAAGAA+AGG + Chr2:19384861-19384880 None:intergenic 25.0%
! ATCTTTAAACAACATTCTCT+AGG - Chr2:19385858-19385877 MsG0280007698.01.T01:intron 25.0%
! ATTTGAAGAAGTAATTCTTC+TGG + Chr2:19385476-19385495 None:intergenic 25.0%
! CTGATAGAATTATTGTAAAC+AGG - Chr2:19385547-19385566 MsG0280007698.01.T01:intron 25.0%
! CTTAGAAAATTGGGTTAAAA+AGG - Chr2:19384089-19384108 MsG0280007698.01.T01:intron 25.0%
! GAATTGAAAATGATAGAAGA+AGG + Chr2:19383878-19383897 None:intergenic 25.0%
! GCATACATTCAAATTGTATA+AGG + Chr2:19384668-19384687 None:intergenic 25.0%
! GTAAACTCCTAATACATTTA+GGG - Chr2:19385598-19385617 MsG0280007698.01.T01:intron 25.0%
! TAGAAAATTGGGTTAAAAAG+GGG - Chr2:19384091-19384110 MsG0280007698.01.T01:intron 25.0%
! TATCGTATGCTTAGAAAATT+GGG - Chr2:19384080-19384099 MsG0280007698.01.T01:intron 25.0%
! TATTTACAGGAAAATCTCTT+AGG - Chr2:19385383-19385402 MsG0280007698.01.T01:intron 25.0%
! TGTAAACTCCTAATACATTT+AGG - Chr2:19385597-19385616 MsG0280007698.01.T01:intron 25.0%
! TTATCGTATGCTTAGAAAAT+TGG - Chr2:19384079-19384098 MsG0280007698.01.T01:intron 25.0%
!! AACTAATATCTAGGACAAAA+TGG + Chr2:19385042-19385061 None:intergenic 25.0%
!! ATTACATTTTGCACGTTTTT+GGG + Chr2:19385996-19386015 None:intergenic 25.0%
!! ATTTTCAATTCACAACACTA+AGG - Chr2:19383887-19383906 MsG0280007698.01.T01:CDS 25.0%
!! CTTTTTAGATTTTCCTTTCT+GGG - Chr2:19384010-19384029 MsG0280007698.01.T01:intron 25.0%
!! TATTACATTTTGCACGTTTT+TGG + Chr2:19385997-19386016 None:intergenic 25.0%
!! TCTTTTTAGATTTTCCTTTC+TGG - Chr2:19384009-19384028 MsG0280007698.01.T01:intron 25.0%
!! TTTGTTTGATAACTAACTTC+AGG + Chr2:19386027-19386046 None:intergenic 25.0%
!!! CAATTCTAAATTTTCAAGCA+AGG + Chr2:19384360-19384379 None:intergenic 25.0%
!!! GAATTTTGACAGAACTTAAA+TGG - Chr2:19384565-19384584 MsG0280007698.01.T01:intron 25.0%
!!! GGTTATGCTTATTGATTTTT+TGG - Chr2:19384268-19384287 MsG0280007698.01.T01:intron 25.0%
!!! GTTTTTTTGTTTCTAGTTGT+AGG - Chr2:19385071-19385090 MsG0280007698.01.T01:intron 25.0%
!!! TTTGTTCCTTTCTTTGATTT+TGG - Chr2:19384852-19384871 MsG0280007698.01.T01:intron 25.0%
!!! TTTTCCTTTACTTGTTTTGT+TGG - Chr2:19384537-19384556 MsG0280007698.01.T01:intron 25.0%
!!! TTTTGGGTTTTTGATTTTCA+TGG - Chr2:19384176-19384195 MsG0280007698.01.T01:intron 25.0%
AAATTCCGTTTGCAGAATAT+AGG + Chr2:19386105-19386124 None:intergenic 30.0%
AAGAGACAGAACTAATATCT+AGG + Chr2:19385051-19385070 None:intergenic 30.0%
AATAAGCAGAAAACAGCTAA+TGG + Chr2:19385703-19385722 None:intergenic 30.0%
ACATAAAACTTCAGATAGCA+TGG + Chr2:19385932-19385951 None:intergenic 30.0%
ATCCATGGCATAATTAATAG+AGG - Chr2:19384640-19384659 MsG0280007698.01.T01:five_prime_UTR 30.0%
CAAAATAGAAAATTGGGAAG+GGG + Chr2:19384161-19384180 None:intergenic 30.0%
CAATCCAACAAAACAAGTAA+AGG + Chr2:19384544-19384563 None:intergenic 30.0%
CCAAAATAGAAAATTGGGAA+GGG + Chr2:19384162-19384181 None:intergenic 30.0%
GCTATGATAAAATTCACACT+TGG + Chr2:19384338-19384357 None:intergenic 30.0%
TAACTGCTATAAACTGTAAC+TGG - Chr2:19383953-19383972 MsG0280007698.01.T01:intron 30.0%
TAATTATGCCATGGATCATA+TGG + Chr2:19384636-19384655 None:intergenic 30.0%
TCCATGGCATAATTAATAGA+GGG - Chr2:19384641-19384660 MsG0280007698.01.T01:five_prime_UTR 30.0%
TGATAACAATCCTAAGAACT+TGG - Chr2:19384942-19384961 MsG0280007698.01.T01:intron 30.0%
! ATTTTCCTGTAAATACCTGT+GGG + Chr2:19385378-19385397 None:intergenic 30.0%
! CCTTCCCAATTTTCTATTTT+GGG - Chr2:19384160-19384179 MsG0280007698.01.T01:intron 30.0%
! TGGGTTTTGCTCAATTTTTA+TGG - Chr2:19384029-19384048 MsG0280007698.01.T01:intron 30.0%
! TTACATTTTGCACGTTTTTG+GGG + Chr2:19385995-19386014 None:intergenic 30.0%
! TTTTCTTCATTTCCTTTTCC+CGG - Chr2:19385234-19385253 MsG0280007698.01.T01:intron 30.0%
!! AATTGAAGTGTTGTTGAGTT+TGG + Chr2:19383847-19383866 None:intergenic 30.0%
!! ACAGAACTTAAATGGATTGA+TGG - Chr2:19384573-19384592 MsG0280007698.01.T01:intron 30.0%
!! ATTGAAGTGTTGTTGAGTTT+GGG + Chr2:19383846-19383865 None:intergenic 30.0%
!! TTTTGAGAGCTTATGAAAAC+CGG - Chr2:19385666-19385685 MsG0280007698.01.T01:intron 30.0%
!!! GGCATTTTTATTTTCTGATG+TGG - Chr2:19385404-19385423 MsG0280007698.01.T01:intron 30.0%
!!! TTTCATGGTTTTTGAGTGAA+AGG - Chr2:19384191-19384210 MsG0280007698.01.T01:intron 30.0%
AAACAGGCCCTAAATGTATT+AGG + Chr2:19385608-19385627 None:intergenic 35.0%
AAATTGAGCAAAACCCAGAA+AGG + Chr2:19384026-19384045 None:intergenic 35.0%
AAATTGTTGTCCAGATAACG+AGG - Chr2:19386044-19386063 MsG0280007698.01.T01:intron 35.0%
AACCTGAGCATTATCAATAC+CGG + Chr2:19386134-19386153 None:intergenic 35.0%
AAGAAAGGAACAAAAGCCAA+AGG + Chr2:19384846-19384865 None:intergenic 35.0%
AGAAAGGAACAAAAGCCAAA+GGG + Chr2:19384845-19384864 None:intergenic 35.0%
AGCAGAACAAACACTGTTAA+CGG + Chr2:19385297-19385316 None:intergenic 35.0%
ATACAATATGTGTGCTGCAT+GGG - Chr2:19384819-19384838 MsG0280007698.01.T01:intron 35.0%
CCCAAAATAGAAAATTGGGA+AGG + Chr2:19384163-19384182 None:intergenic 35.0%
CTTCTTCAAATCATGGTTGT+CGG - Chr2:19385485-19385504 MsG0280007698.01.T01:intron 35.0%
GACAAAATCACCAAGTTCTT+AGG + Chr2:19384955-19384974 None:intergenic 35.0%
GAGATTGAGATTTGACATGT+TGG - Chr2:19384303-19384322 MsG0280007698.01.T01:intron 35.0%
TCAAACAAGTCAATCCAAAC+AGG + Chr2:19385624-19385643 None:intergenic 35.0%
TCCAAAATCCATATGATCCA+TGG - Chr2:19384625-19384644 MsG0280007698.01.T01:exon 35.0%
TGCATCATTATAATCCTGCA+TGG - Chr2:19384994-19385013 MsG0280007698.01.T01:intron 35.0%
! ACTTATGCTGTCTCTTTTAG+TGG - Chr2:19384710-19384729 MsG0280007698.01.T01:intron 35.0%
! CCCTTCCCAATTTTCTATTT+TGG - Chr2:19384159-19384178 MsG0280007698.01.T01:intron 35.0%
! GATTTTCCTGTAAATACCTG+TGG + Chr2:19385379-19385398 None:intergenic 35.0%
! GGATCATATGGATTTTGGAT+TGG + Chr2:19384624-19384643 None:intergenic 35.0%
! TAATACATTTAGGGCCTGTT+TGG - Chr2:19385607-19385626 MsG0280007698.01.T01:intron 35.0%
!! ACGACTGTGCTCTAAAAATT+TGG + Chr2:19385106-19385125 None:intergenic 35.0%
ATAGAACAGAATCCGGGAAA+AGG + Chr2:19385249-19385268 None:intergenic 40.0%
ATCATGTGTGGAAGAAGTCA+TGG + Chr2:19385355-19385374 None:intergenic 40.0%
ATGATCCCACAGGTATTTAC+AGG - Chr2:19385370-19385389 MsG0280007698.01.T01:intron 40.0%
CATACAATATGTGTGCTGCA+TGG - Chr2:19384818-19384837 MsG0280007698.01.T01:intron 40.0%
CATGACATTGCAGTGAATCA+TGG - Chr2:19385135-19385154 MsG0280007698.01.T01:intron 40.0%
GCCCTCTATTAATTATGCCA+TGG + Chr2:19384645-19384664 None:intergenic 40.0%
GTTTGTTCTGCTTCCAGATT+TGG - Chr2:19385305-19385324 MsG0280007698.01.T01:intron 40.0%
TGCAGCCTATATTCTGCAAA+CGG - Chr2:19386097-19386116 MsG0280007698.01.T01:five_prime_UTR 40.0%
TTGGAAATCTGAACTTGAGC+TGG - Chr2:19384871-19384890 MsG0280007698.01.T01:intron 40.0%
! GCCATGGATCATATGGATTT+TGG + Chr2:19384629-19384648 None:intergenic 40.0%
! GCTAATGGACAAGTCATAAC+CGG + Chr2:19385688-19385707 None:intergenic 40.0%
!! ATAGGACTAGTCAAAGGCAT+CGG + Chr2:19385965-19385984 None:intergenic 40.0%
AATACCTGTGGGATCATGTG+TGG + Chr2:19385367-19385386 None:intergenic 45.0%
ACATGCACTCTGTCCAAATC+TGG + Chr2:19385321-19385340 None:intergenic 45.0%
CTCCGTCATAGAACAGAATC+CGG + Chr2:19385256-19385275 None:intergenic 45.0%
GACAAAATGGAGCCCACAAA+AGG + Chr2:19385029-19385048 None:intergenic 45.0%
TCCCGGATTCTGTTCTATGA+CGG - Chr2:19385251-19385270 MsG0280007698.01.T01:intron 45.0%
TCCGTCATAGAACAGAATCC+GGG + Chr2:19385255-19385274 None:intergenic 45.0%
! CTTTGACTAGTCCTATTCCC+TGG - Chr2:19385969-19385988 MsG0280007698.01.T01:intron 45.0%
! TATTGCAGCTAAGAGAGCAC+AGG + Chr2:19384742-19384761 None:intergenic 45.0%
! TGTTGTTGAGTTTGGGACAG+TGG + Chr2:19383839-19383858 None:intergenic 45.0%
!! ATGGCTCTATCTGCCTTTTG+TGG - Chr2:19385013-19385032 MsG0280007698.01.T01:intron 45.0%
!! CAGGGAATAGGACTAGTCAA+AGG + Chr2:19385971-19385990 None:intergenic 45.0%
!! TGGCTCTATCTGCCTTTTGT+GGG - Chr2:19385014-19385033 MsG0280007698.01.T01:intron 45.0%
AAAGGCAGATAGAGCCATGC+AGG + Chr2:19385011-19385030 None:intergenic 50.0%
ATCCCTTGCACTTGTGCTCA+GGG - Chr2:19384905-19384924 MsG0280007698.01.T01:intron 50.0%
GCAAACGGAATTTCTCACGC+CGG - Chr2:19386112-19386131 MsG0280007698.01.T01:five_prime_UTR 50.0%
GGACAGAGTGCATGTGAATC+AGG - Chr2:19385326-19385345 MsG0280007698.01.T01:intron 50.0%
TCTTCCACACATGATCCCAC+AGG - Chr2:19385360-19385379 MsG0280007698.01.T01:intron 50.0%
TGTCCAGATAACGAGGCTGA+TGG - Chr2:19386051-19386070 MsG0280007698.01.T01:intron 50.0%
TGTCCATCAGCCTCGTTATC+TGG + Chr2:19386057-19386076 None:intergenic 50.0%
TTCCCTGAGCACAAGTGCAA+GGG + Chr2:19384910-19384929 None:intergenic 50.0%
!! CGCCGGTATTGATAATGCTC+AGG - Chr2:19386129-19386148 MsG0280007698.01.T01:five_prime_UTR 50.0%
!!! TTTGCACGTTTTTGGGGCCA+GGG + Chr2:19385989-19386008 None:intergenic 50.0%
!!! TTTTGCACGTTTTTGGGGCC+AGG + Chr2:19385990-19386009 None:intergenic 50.0%
CATCCCTTGCACTTGTGCTC+AGG - Chr2:19384904-19384923 MsG0280007698.01.T01:intron 55.0%
GTTCCCTGAGCACAAGTGCA+AGG + Chr2:19384911-19384930 None:intergenic 55.0%
TATGCAGCACCTCCACAGCT+TGG - Chr2:19386160-19386179 MsG0280007698.01.T01:five_prime_UTR 55.0%
!!! CGTTTTTGGGGCCAGGGAAT+AGG + Chr2:19385983-19386002 None:intergenic 55.0%
AGCACCTCCACAGCTTGGCA+CGG - Chr2:19386165-19386184 MsG0280007698.01.T01:five_prime_UTR 60.0%
TGTGTGCTGCATGGGCCCTT+TGG - Chr2:19384827-19384846 MsG0280007698.01.T01:intron 60.0%
CACCTCCACAGCTTGGCACG+GGG - Chr2:19386167-19386186 MsG0280007698.01.T01:five_prime_UTR 65.0%
GCACCTCCACAGCTTGGCAC+GGG - Chr2:19386166-19386185 MsG0280007698.01.T01:five_prime_UTR 65.0%
GCTTGGCACGGGGCATGCTA+TGG - Chr2:19386177-19386196 MsG0280007698.01.T01:five_prime_UTR 65.0%
GCATGCCCCGTGCCAAGCTG+TGG + Chr2:19386175-19386194 None:intergenic 70.0%
TGCCCCGTGCCAAGCTGTGG+AGG + Chr2:19386172-19386191 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
Chr2 gene 19383780 19386204 19383780 ID=MsG0280007698.01;Name=MsG0280007698.01
Chr2 mRNA 19383780 19386204 19383780 ID=MsG0280007698.01.T01;Parent=MsG0280007698.01;Name=MsG0280007698.01.T01;_AED=0.48;_eAED=0.51;_QI=161|0.5|0.66|1|0|0|3|0|65
Chr2 exon 19383780 19383949 19383780 ID=MsG0280007698.01.T01:exon:4536;Parent=MsG0280007698.01.T01
Chr2 exon 19384603 19384662 19384603 ID=MsG0280007698.01.T01:exon:4535;Parent=MsG0280007698.01.T01
Chr2 exon 19386076 19386204 19386076 ID=MsG0280007698.01.T01:exon:4534;Parent=MsG0280007698.01.T01
Chr2 five_prime_UTR 19386076 19386204 19386076 ID=MsG0280007698.01.T01:five_prime_utr;Parent=MsG0280007698.01.T01
Chr2 five_prime_UTR 19384631 19384662 19384631 ID=MsG0280007698.01.T01:five_prime_utr;Parent=MsG0280007698.01.T01
Chr2 CDS 19384603 19384630 19384603 ID=MsG0280007698.01.T01:cds;Parent=MsG0280007698.01.T01
Chr2 CDS 19383780 19383949 19383780 ID=MsG0280007698.01.T01:cds;Parent=MsG0280007698.01.T01
Gene Sequence

>MsG0280007698.01.T01

ATGACTTCTTCCACACATGATCCCACAGTTATCAAACAAATTGTTGTCCAGATAACGAGGCTGATGGACAGCAGCAGTCAGAATCACAGATGCAGCCTATATTCTGCAAACGGAATTTCTCACGCCGGTATTGATAATGCTCAGGTTGTTCAGTATGCAGCACCTCCACAGCTTGGCACGGGGCATGCTATGGTATGA

Protein sequence

>MsG0280007698.01.T01

MTSSTHDPTVIKQIVVQITRLMDSSSQNHRCSLYSANGISHAGIDNAQVVQYAAPPQLGTGHAMV*