Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380016020.01.T01 | XP_013461159.2 | 92.973 | 185 | 11 | 2 | 1 | 184 | 1 | 184 | 7.66E-85 | 259 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380016020.01.T01 | Q9LIE6 | 71.667 | 60 | 16 | 1 | 57 | 115 | 41 | 100 | 8.01E-12 | 64.3 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380016020.01.T01 | A0A396IXD1 | 92.973 | 185 | 11 | 2 | 1 | 184 | 1 | 184 | 3.66e-85 | 259 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048036.01 | MsG0380016020.01 | 0.867557 | 1.253801e-65 | 4.396190e-62 |
| MsG0080048347.01 | MsG0380016020.01 | 0.826169 | 3.144296e-54 | 2.926258e-51 |
| MsG0080048352.01 | MsG0380016020.01 | 0.819730 | 9.959655e-53 | 7.727179e-50 |
| MsG0080048967.01 | MsG0380016020.01 | 0.828133 | 1.065163e-54 | 1.049380e-51 |
| MsG0080049066.01 | MsG0380016020.01 | -0.806551 | 7.787357e-50 | 4.239046e-47 |
| MsG0080049128.01 | MsG0380016020.01 | 0.803785 | 2.953865e-49 | 1.497696e-46 |
| MsG0080049133.01 | MsG0380016020.01 | -0.800711 | 1.267612e-48 | 5.943171e-46 |
| MsG0180000234.01 | MsG0380016020.01 | 0.832101 | 1.145743e-55 | 1.267836e-52 |
| MsG0180001050.01 | MsG0380016020.01 | 0.825982 | 3.482113e-54 | 3.223585e-51 |
| MsG0180001111.01 | MsG0380016020.01 | -0.836105 | 1.137333e-56 | 1.419062e-53 |
| MsG0180002214.01 | MsG0380016020.01 | -0.802305 | 5.974245e-49 | 2.916797e-46 |
| MsG0180003240.01 | MsG0380016020.01 | 0.872429 | 3.201171e-67 | 1.339293e-63 |
| MsG0180003757.01 | MsG0380016020.01 | -0.833468 | 5.240598e-56 | 6.040639e-53 |
| MsG0180003779.01 | MsG0380016020.01 | -0.800932 | 1.142635e-48 | 5.387763e-46 |
| MsG0180004203.01 | MsG0380016020.01 | 0.856621 | 2.842137e-62 | 6.824271e-59 |
| MsG0180004328.01 | MsG0380016020.01 | 0.843209 | 1.612559e-58 | 2.504677e-55 |
| MsG0180004491.01 | MsG0380016020.01 | 0.837638 | 4.618358e-57 | 6.036873e-54 |
| MsG0180004502.01 | MsG0380016020.01 | -0.812810 | 3.515910e-51 | 2.257266e-48 |
| MsG0180004562.01 | MsG0380016020.01 | 0.803516 | 3.358840e-49 | 1.691316e-46 |
| MsG0180005328.01 | MsG0380016020.01 | 0.838591 | 2.626318e-57 | 3.534293e-54 |
| MsG0180005356.01 | MsG0380016020.01 | 0.809466 | 1.865983e-50 | 1.096388e-47 |
| MsG0180005542.01 | MsG0380016020.01 | 0.828267 | 9.883738e-55 | 9.774758e-52 |
| MsG0180005648.01 | MsG0380016020.01 | 0.825293 | 5.072235e-54 | 4.604370e-51 |
| MsG0280006452.01 | MsG0380016020.01 | -0.837508 | 4.987914e-57 | 6.493616e-54 |
| MsG0280010802.01 | MsG0380016020.01 | 0.843067 | 1.759386e-58 | 2.720475e-55 |
| MsG0280011041.01 | MsG0380016020.01 | 0.830160 | 3.433536e-55 | 3.588623e-52 |
| MsG0280011042.01 | MsG0380016020.01 | 0.835930 | 1.259453e-56 | 1.563019e-53 |
| MsG0280011165.01 | MsG0380016020.01 | 0.819485 | 1.132930e-52 | 8.730036e-50 |
| MsG0280011193.01 | MsG0380016020.01 | 0.817900 | 2.594228e-52 | 1.913293e-49 |
| MsG0280011203.01 | MsG0380016020.01 | 0.813518 | 2.458573e-51 | 1.609068e-48 |
| MsG0280011301.01 | MsG0380016020.01 | -0.810068 | 1.385052e-50 | 8.268468e-48 |
| MsG0380011613.01 | MsG0380016020.01 | 0.839250 | 1.773328e-57 | 2.434711e-54 |
| MsG0380012281.01 | MsG0380016020.01 | -0.810803 | 9.611486e-51 | 5.850425e-48 |
| MsG0380013114.01 | MsG0380016020.01 | -0.822499 | 2.292951e-53 | 1.922593e-50 |
| MsG0380013300.01 | MsG0380016020.01 | 0.856375 | 3.356657e-62 | 7.995305e-59 |
| MsG0380013449.01 | MsG0380016020.01 | 0.803605 | 3.218086e-49 | 1.624191e-46 |
| MsG0380013452.01 | MsG0380016020.01 | 0.814430 | 1.546986e-51 | 1.037812e-48 |
| MsG0380013458.01 | MsG0380016020.01 | 0.803226 | 3.856191e-49 | 1.927270e-46 |
| MsG0380014934.01 | MsG0380016020.01 | 0.804855 | 1.768210e-49 | 9.216065e-47 |
| MsG0380015010.01 | MsG0380016020.01 | 0.827828 | 1.261059e-54 | 1.231265e-51 |
| MsG0380015070.01 | MsG0380016020.01 | 0.802387 | 5.746746e-49 | 2.811553e-46 |
| MsG0380015201.01 | MsG0380016020.01 | -0.818709 | 1.701884e-52 | 1.283426e-49 |
| MsG0380015369.01 | MsG0380016020.01 | 0.809851 | 1.542609e-50 | 9.156641e-48 |
| MsG0380015619.01 | MsG0380016020.01 | -0.803766 | 2.980669e-49 | 1.510537e-46 |
| MsG0380015629.01 | MsG0380016020.01 | 0.803195 | 3.913729e-49 | 1.954539e-46 |
| MsG0380015752.01 | MsG0380016020.01 | -0.804251 | 2.362401e-49 | 1.212167e-46 |
| MsG0380015971.01 | MsG0380016020.01 | -0.808833 | 2.550056e-50 | 1.473166e-47 |
| MsG0380016006.01 | MsG0380016020.01 | 0.848246 | 6.926662e-60 | 1.263211e-56 |
| MsG0380016020.01 | MsG0380016790.01 | -0.803916 | 2.774460e-49 | 1.411549e-46 |
| MsG0380016020.01 | MsG0380016801.01 | 0.803972 | 2.700698e-49 | 1.375956e-46 |
| MsG0380016020.01 | MsG0380016835.01 | -0.807282 | 5.454372e-50 | 3.025799e-47 |
| MsG0380016020.01 | MsG0380016839.01 | 0.819861 | 9.296950e-53 | 7.239475e-50 |
| MsG0380016020.01 | MsG0380016931.01 | -0.806184 | 9.306452e-50 | 5.018818e-47 |
| MsG0380016020.01 | MsG0380017115.01 | 0.853296 | 2.624839e-61 | 5.644328e-58 |
| MsG0380016020.01 | MsG0380017122.01 | 0.825443 | 4.674803e-54 | 4.261298e-51 |
| MsG0380016020.01 | MsG0380017151.01 | 0.806695 | 7.260060e-50 | 3.966513e-47 |
| MsG0380016020.01 | MsG0380017405.01 | -0.807579 | 4.717598e-50 | 2.637145e-47 |
| MsG0380016020.01 | MsG0380017690.01 | 0.843614 | 1.257571e-58 | 1.978700e-55 |
| MsG0380016020.01 | MsG0380017754.01 | 0.804499 | 2.097762e-49 | 1.083264e-46 |
| MsG0380016020.01 | MsG0380017962.01 | -0.809135 | 2.197066e-50 | 1.279636e-47 |
| MsG0380016020.01 | MsG0480018628.01 | -0.819750 | 9.858597e-53 | 7.652887e-50 |
| MsG0380016020.01 | MsG0480018768.01 | 0.849937 | 2.345664e-60 | 4.520269e-57 |
| MsG0380016020.01 | MsG0480020497.01 | 0.828724 | 7.668729e-55 | 7.685387e-52 |
| MsG0380016020.01 | MsG0480020784.01 | 0.816049 | 6.757537e-52 | 4.737799e-49 |
| MsG0380016020.01 | MsG0480021327.01 | -0.824779 | 6.710051e-54 | 6.002562e-51 |
| MsG0380016020.01 | MsG0480021331.01 | -0.817954 | 2.521930e-52 | 1.862736e-49 |
| MsG0380016020.01 | MsG0480022125.01 | 0.826088 | 3.286541e-54 | 3.051504e-51 |
| MsG0380016020.01 | MsG0480022638.01 | 0.841446 | 4.729569e-58 | 6.951794e-55 |
| MsG0380016020.01 | MsG0480022655.01 | 0.801576 | 8.434339e-49 | 4.042008e-46 |
| MsG0380016020.01 | MsG0480023102.01 | 0.806203 | 9.221586e-50 | 4.975498e-47 |
| MsG0380016020.01 | MsG0480023187.01 | -0.830311 | 3.153763e-55 | 3.310474e-52 |
| MsG0380016020.01 | MsG0480023349.01 | 0.822730 | 2.026002e-53 | 1.709981e-50 |
| MsG0380016020.01 | MsG0480023921.01 | 0.854195 | 1.447291e-61 | 3.207023e-58 |
| MsG0380016020.01 | MsG0580024408.01 | 0.851583 | 8.076912e-61 | 1.642366e-57 |
| MsG0380016020.01 | MsG0580025683.01 | 0.829545 | 4.849725e-55 | 4.978733e-52 |
| MsG0380016020.01 | MsG0580027227.01 | 0.814933 | 1.196718e-51 | 8.137631e-49 |
| MsG0380016020.01 | MsG0580027646.01 | 0.835297 | 1.821100e-56 | 2.217908e-53 |
| MsG0380016020.01 | MsG0580028560.01 | 0.811277 | 7.588815e-51 | 4.677350e-48 |
| MsG0380016020.01 | MsG0580028999.01 | 0.808656 | 2.782455e-50 | 1.599943e-47 |
| MsG0380016020.01 | MsG0580029555.01 | 0.876025 | 1.938527e-68 | 9.252973e-65 |
| MsG0380016020.01 | MsG0580029626.01 | 0.842340 | 2.745896e-58 | 4.150700e-55 |
| MsG0380016020.01 | MsG0580030133.01 | 0.848456 | 6.057974e-60 | 1.112462e-56 |
| MsG0380016020.01 | MsG0580030135.01 | 0.838447 | 2.860572e-57 | 3.832615e-54 |
| MsG0380016020.01 | MsG0580030197.01 | -0.817887 | 2.611889e-52 | 1.925662e-49 |
| MsG0380016020.01 | MsG0680030336.01 | 0.824737 | 6.864052e-54 | 6.133071e-51 |
| MsG0380016020.01 | MsG0680031240.01 | 0.830995 | 2.145346e-55 | 2.297679e-52 |
| MsG0380016020.01 | MsG0680031429.01 | 0.838834 | 2.271760e-57 | 3.079694e-54 |
| MsG0380016020.01 | MsG0680031813.01 | -0.809197 | 2.131170e-50 | 1.243303e-47 |
| MsG0380016020.01 | MsG0680034229.01 | -0.831760 | 1.390671e-55 | 1.523799e-52 |
| MsG0380016020.01 | MsG0680035496.01 | 0.805752 | 1.147074e-49 | 6.118242e-47 |
| MsG0380016020.01 | MsG0680035841.01 | 0.835928 | 1.261380e-56 | 1.565277e-53 |
| MsG0380016020.01 | MsG0780036661.01 | 0.820971 | 5.173460e-53 | 4.155003e-50 |
| MsG0380016020.01 | MsG0780038922.01 | 0.801895 | 7.256903e-49 | 3.506059e-46 |
| MsG0380016020.01 | MsG0780039334.01 | 0.825475 | 4.594961e-54 | 4.192387e-51 |
| MsG0380016020.01 | MsG0780039783.01 | 0.819586 | 1.074332e-52 | 8.301876e-50 |
| MsG0380016020.01 | MsG0780039839.01 | -0.821268 | 4.418665e-53 | 3.577696e-50 |
| MsG0380016020.01 | MsG0780039877.01 | 0.814243 | 1.701327e-51 | 1.135581e-48 |
| MsG0380016020.01 | MsG0780039968.01 | 0.852017 | 6.081139e-61 | 1.253586e-57 |
| MsG0380016020.01 | MsG0780040121.01 | -0.804379 | 2.222571e-49 | 1.144234e-46 |
| MsG0380016020.01 | MsG0780040181.01 | 0.803418 | 3.518780e-49 | 1.767382e-46 |
| MsG0380016020.01 | MsG0780040321.01 | -0.801942 | 7.096182e-49 | 3.432324e-46 |
| MsG0380016020.01 | MsG0780041177.01 | 0.817016 | 4.103298e-52 | 2.954043e-49 |
| MsG0380016020.01 | MsG0780041254.01 | -0.814209 | 1.731375e-51 | 1.154482e-48 |
| MsG0380016020.01 | MsG0780041316.01 | 0.800474 | 1.417107e-48 | 6.604303e-46 |
| MsG0380016020.01 | MsG0780041380.01 | 0.840121 | 1.052280e-57 | 1.484331e-54 |
| MsG0380016020.01 | MsG0780041556.01 | 0.820672 | 6.061658e-53 | 4.827084e-50 |
| MsG0380016020.01 | MsG0780041574.01 | 0.820062 | 8.363423e-53 | 6.549589e-50 |
| MsG0380016020.01 | MsG0780041620.01 | -0.802609 | 5.172824e-49 | 2.544917e-46 |
| MsG0380016020.01 | MsG0780041720.01 | -0.821810 | 3.312625e-53 | 2.723583e-50 |
| MsG0380016020.01 | MsG0780041783.01 | -0.818435 | 1.963711e-52 | 1.469909e-49 |
| MsG0380016020.01 | MsG0880041855.01 | -0.817069 | 3.991223e-52 | 2.877475e-49 |
| MsG0380016020.01 | MsG0880042740.01 | 0.825060 | 5.757633e-54 | 5.192042e-51 |
| MsG0380016020.01 | MsG0880042858.01 | -0.821727 | 3.461453e-53 | 2.839086e-50 |
| MsG0380016020.01 | MsG0880043364.01 | 0.819584 | 1.075560e-52 | 8.310937e-50 |
| MsG0380016020.01 | MsG0880043744.01 | 0.827183 | 1.800680e-54 | 1.725562e-51 |
| MsG0380016020.01 | MsG0880043824.01 | 0.811224 | 7.791828e-51 | 4.796016e-48 |
| MsG0380016020.01 | MsG0880043828.01 | 0.849020 | 4.228133e-60 | 7.908351e-57 |
| MsG0380016020.01 | MsG0880045188.01 | -0.801090 | 1.060908e-48 | 5.022559e-46 |
| MsG0380016020.01 | MsG0880045272.01 | 0.814242 | 1.701834e-51 | 1.135897e-48 |
| MsG0380016020.01 | MsG0880045277.01 | 0.824163 | 9.368377e-54 | 8.233336e-51 |
| MsG0380016020.01 | MsG0880045677.01 | 0.808234 | 3.422636e-50 | 1.946593e-47 |
| MsG0380016020.01 | MsG0880046429.01 | 0.847326 | 1.241497e-59 | 2.198500e-56 |
| MsG0380016020.01 | MsG0880046469.01 | -0.808217 | 3.451701e-50 | 1.962241e-47 |
| MsG0380016020.01 | MsG0880046996.01 | 0.860588 | 1.860036e-63 | 5.111047e-60 |
| MsG0380016020.01 | MsG0880047256.01 | 0.843037 | 1.792140e-58 | 2.768433e-55 |
| MsG0380016020.01 | MsG0880047318.01 | -0.811642 | 6.322252e-51 | 3.934328e-48 |
| MsG0380016020.01 | MsG0880047665.01 | 0.846394 | 2.232212e-59 | 3.836886e-56 |
| MsG0380016020.01 | MsG0880047747.01 | 0.873012 | 2.044062e-67 | 8.733366e-64 |
| MsG0280007216.01 | MsG0380016020.01 | 0.819138 | 1.359554e-52 | 1.037469e-49 |
| MsG0280007649.01 | MsG0380016020.01 | -0.811284 | 7.562593e-51 | 4.662111e-48 |
| MsG0280007650.01 | MsG0380016020.01 | -0.813076 | 3.073656e-51 | 1.987460e-48 |
| MsG0280008405.01 | MsG0380016020.01 | 0.834662 | 2.633258e-56 | 3.145645e-53 |
| MsG0280008601.01 | MsG0380016020.01 | 0.816744 | 4.721497e-52 | 3.373701e-49 |
| MsG0280008747.01 | MsG0380016020.01 | 0.803435 | 3.491471e-49 | 1.754379e-46 |
| MsG0280009394.01 | MsG0380016020.01 | 0.811362 | 7.270730e-51 | 4.491480e-48 |
| MsG0280010281.01 | MsG0380016020.01 | -0.805220 | 1.482889e-49 | 7.801321e-47 |
| MsG0280010521.01 | MsG0380016020.01 | 0.804326 | 2.279008e-49 | 1.171684e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380016020.01.T01 | MTR_3g081500 | 92.818 | 181 | 11 | 2 | 5 | 184 | 1 | 180 | 1.65e-85 | 251 |
| MsG0380016020.01.T01 | MTR_2g035850 | 44.037 | 109 | 55 | 3 | 1 | 103 | 1 | 109 | 1.46e-18 | 80.5 |
| MsG0380016020.01.T01 | MTR_4g094698 | 38.509 | 161 | 70 | 8 | 2 | 157 | 4 | 140 | 1.02e-15 | 71.2 |
| MsG0380016020.01.T01 | MTR_5g063310 | 62.791 | 43 | 16 | 0 | 71 | 113 | 155 | 197 | 1.72e-11 | 62.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380016020.01.T01 | AT4G39720 | 34.307 | 137 | 86 | 3 | 13 | 147 | 56 | 190 | 6.63e-13 | 65.9 |
| MsG0380016020.01.T01 | AT4G39720 | 34.307 | 137 | 86 | 3 | 13 | 147 | 56 | 190 | 6.79e-13 | 65.9 |
| MsG0380016020.01.T01 | AT4G39720 | 34.307 | 137 | 86 | 3 | 13 | 147 | 56 | 190 | 7.19e-13 | 65.9 |
| MsG0380016020.01.T01 | AT4G39720 | 34.307 | 137 | 86 | 3 | 13 | 147 | 56 | 190 | 7.19e-13 | 65.9 |
| MsG0380016020.01.T01 | AT3G22160 | 71.667 | 60 | 16 | 1 | 57 | 115 | 41 | 100 | 8.16e-13 | 64.3 |
| MsG0380016020.01.T01 | AT1G35830 | 71.053 | 38 | 11 | 0 | 71 | 108 | 100 | 137 | 1.45e-11 | 62.0 |
| MsG0380016020.01.T01 | AT5G65170 | 62.791 | 43 | 16 | 0 | 71 | 113 | 140 | 182 | 2.69e-11 | 61.6 |
| MsG0380016020.01.T01 | AT4G15120 | 80.851 | 47 | 9 | 0 | 59 | 105 | 39 | 85 | 2.70e-11 | 60.1 |
Find 60 sgRNAs with CRISPR-Local
Find 69 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGATTCATCATGGTTGATTC+AGG | 0.196823 | 3:-77999621 | None:intergenic |
| GTGTAAGGAGGGGTTTGATT+AGG | 0.284979 | 3:-77999558 | None:intergenic |
| CTGGCTGAGGTTGGTGAGCT+TGG | 0.290535 | 3:-77999642 | None:intergenic |
| TGTAAGGAGGGGTTTGATTA+GGG | 0.309491 | 3:-77999557 | None:intergenic |
| ACCCTAGCAGGCCTAGGGCT+TGG | 0.349369 | 3:+77999855 | MsG0380016020.01.T01:CDS |
| TTCAGATGGACCCAAGTTGC+TGG | 0.349830 | 3:-77999661 | None:intergenic |
| CCTGCTAGGGTTGAGAAATT+AGG | 0.351690 | 3:-77999843 | None:intergenic |
| AGGCCTAGGGCTTGGCCCAC+GGG | 0.363073 | 3:+77999863 | MsG0380016020.01.T01:CDS |
| CAGGCCTAGGGCTTGGCCCA+CGG | 0.392030 | 3:+77999862 | MsG0380016020.01.T01:CDS |
| GCTGCTGCAAAAGGTGCAAT+AGG | 0.418378 | 3:-77999801 | None:intergenic |
| GACATCATAGATTGATTCAT+AGG | 0.420321 | 3:-77999894 | None:intergenic |
| GATTCATCATGGTTGATTCA+GGG | 0.429834 | 3:-77999620 | None:intergenic |
| GTTGTTGTTGAAGCTGTTGT+TGG | 0.432601 | 3:-77999929 | None:intergenic |
| AGCCCTTGATCTTCTTCGTA+TGG | 0.433702 | 3:-77999700 | None:intergenic |
| TTCTCAACCCTAGCAGGCCT+AGG | 0.444711 | 3:+77999849 | MsG0380016020.01.T01:CDS |
| GAGCCCGTGGGCCAAGCCCT+AGG | 0.445070 | 3:-77999866 | None:intergenic |
| GGCCAAGCCCTAGGCCTGCT+AGG | 0.451902 | 3:-77999857 | None:intergenic |
| TTAGTAGTAATGGAAGTGTA+AGG | 0.460397 | 3:-77999573 | None:intergenic |
| GCCCTTGATCTTCTTCGTAT+GGG | 0.464225 | 3:-77999699 | None:intergenic |
| TAGTAATGGAAGTGTAAGGA+GGG | 0.468900 | 3:-77999569 | None:intergenic |
| CCTAATTTCTCAACCCTAGC+AGG | 0.472155 | 3:+77999843 | MsG0380016020.01.T01:CDS |
| GATGTGGCTGCTGCTGCAAA+AGG | 0.475844 | 3:-77999810 | None:intergenic |
| TGAAGCTGTTGTTGGTAGAA+AGG | 0.476011 | 3:-77999921 | None:intergenic |
| CAACACTACAATATGTATAG+TGG | 0.476494 | 3:+77999975 | MsG0380016020.01.T01:CDS |
| ACCAACCTCAGCCAGCAACT+TGG | 0.483517 | 3:+77999650 | MsG0380016020.01.T01:CDS |
| TTCGTATGGGCTTTGACACA+CGG | 0.492946 | 3:-77999686 | None:intergenic |
| TCATAGATTGATTCATAGGA+TGG | 0.502434 | 3:-77999890 | None:intergenic |
| GCCAAGCCCTAGGCCTGCTA+GGG | 0.502498 | 3:-77999856 | None:intergenic |
| AGGGGTGGTGTTAGTAGTAA+TGG | 0.506379 | 3:-77999583 | None:intergenic |
| CCCAAGTTGCTGGCTGAGGT+TGG | 0.527272 | 3:-77999651 | None:intergenic |
| CCAACCTCAGCCAGCAACTT+GGG | 0.528451 | 3:+77999651 | MsG0380016020.01.T01:CDS |
| GAAGAAGATCAAGGGCTTCG+CGG | 0.529169 | 3:+77999706 | MsG0380016020.01.T01:CDS |
| CATAGATTGATTCATAGGAT+GGG | 0.531126 | 3:-77999889 | None:intergenic |
| AGCCCATACGAAGAAGATCA+AGG | 0.531159 | 3:+77999697 | MsG0380016020.01.T01:CDS |
| CACTACTAATTTCCGTGCCA+TGG | 0.531867 | 3:+77999758 | MsG0380016020.01.T01:CDS |
| GTAGTAATGGAAGTGTAAGG+AGG | 0.532755 | 3:-77999570 | None:intergenic |
| TTCATAGGATGGGGAGCCCG+TGG | 0.535371 | 3:-77999879 | None:intergenic |
| AGCAACTTGGGTCCATCTGA+AGG | 0.541645 | 3:+77999663 | MsG0380016020.01.T01:CDS |
| TCTCAACCCTAGCAGGCCTA+GGG | 0.552947 | 3:+77999850 | MsG0380016020.01.T01:CDS |
| AAAAGGTGCAATAGGACCTC+CGG | 0.561409 | 3:-77999793 | None:intergenic |
| TGGTGAGCTTGGATTCATCA+TGG | 0.561975 | 3:-77999631 | None:intergenic |
| TGGACCCAAGTTGCTGGCTG+AGG | 0.570124 | 3:-77999655 | None:intergenic |
| TTTGACACACGGCCTTCAGA+TGG | 0.574107 | 3:-77999675 | None:intergenic |
| ATAGATTGATTCATAGGATG+GGG | 0.589184 | 3:-77999888 | None:intergenic |
| AGTAATGGAAGTGTAAGGAG+GGG | 0.590782 | 3:-77999568 | None:intergenic |
| GGGTAAAGTTGAAGCAGGGG+TGG | 0.597668 | 3:-77999598 | None:intergenic |
| TCATAGGATGGGGAGCCCGT+GGG | 0.600664 | 3:-77999878 | None:intergenic |
| GCCCATACGAAGAAGATCAA+GGG | 0.605404 | 3:+77999698 | MsG0380016020.01.T01:CDS |
| GCCATGGTACAACAATTCAC+CGG | 0.607187 | 3:+77999774 | MsG0380016020.01.T01:CDS |
| TTCATCATGGTTGATTCAGG+GGG | 0.612844 | 3:-77999618 | None:intergenic |
| AACGTTAATACACACGTAGG+AGG | 0.614425 | 3:+77999999 | MsG0380016020.01.T01:CDS |
| ATTCATCATGGTTGATTCAG+GGG | 0.614825 | 3:-77999619 | None:intergenic |
| TGAATTGTTGTACCATGGCA+CGG | 0.621886 | 3:-77999770 | None:intergenic |
| TCCGGTGAATTGTTGTACCA+TGG | 0.630340 | 3:-77999775 | None:intergenic |
| GCCGACGACTTTACTTAACA+CGG | 0.631878 | 3:+77999734 | MsG0380016020.01.T01:CDS |
| ATTAGGTGGTGATGATGATG+TGG | 0.666182 | 3:-77999826 | None:intergenic |
| GCTAGGGTTGAGAAATTAGG+TGG | 0.673876 | 3:-77999840 | None:intergenic |
| TCCGTGTTAAGTAAAGTCGT+CGG | 0.679295 | 3:-77999735 | None:intergenic |
| ATGGTACAACAATTCACCGG+AGG | 0.714463 | 3:+77999777 | MsG0380016020.01.T01:CDS |
| GGCAACGTTAATACACACGT+AGG | 0.776593 | 3:+77999996 | MsG0380016020.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAGAATTGAAAAAACTCATT+TGG | - | Chr3:77999525-77999544 | None:intergenic | 20.0% |
| ! | AATTGAAAAAACTCATTTGG+TGG | - | Chr3:77999522-77999541 | None:intergenic | 25.0% |
| ! | ATTGAAAAAACTCATTTGGT+GGG | - | Chr3:77999521-77999540 | None:intergenic | 25.0% |
| CAACACTACAATATGTATAG+TGG | + | Chr3:77999975-77999994 | MsG0380016020.01.T01:CDS | 30.0% | |
| ! | ATAGATTGATTCATAGGATG+GGG | - | Chr3:77999891-77999910 | None:intergenic | 30.0% |
| ! | CATAGATTGATTCATAGGAT+GGG | - | Chr3:77999892-77999911 | None:intergenic | 30.0% |
| ! | GACATCATAGATTGATTCAT+AGG | - | Chr3:77999897-77999916 | None:intergenic | 30.0% |
| ! | TCATAGATTGATTCATAGGA+TGG | - | Chr3:77999893-77999912 | None:intergenic | 30.0% |
| ! | TTAGTAGTAATGGAAGTGTA+AGG | - | Chr3:77999576-77999595 | None:intergenic | 30.0% |
| ! | TTGAAAAAACTCATTTGGTG+GGG | - | Chr3:77999520-77999539 | None:intergenic | 30.0% |
| ! | ATTCATCATGGTTGATTCAG+GGG | - | Chr3:77999622-77999641 | None:intergenic | 35.0% |
| ! | GATTCATCATGGTTGATTCA+GGG | - | Chr3:77999623-77999642 | None:intergenic | 35.0% |
| ! | TAGTAATGGAAGTGTAAGGA+GGG | - | Chr3:77999572-77999591 | None:intergenic | 35.0% |
| ! | TGAAAAAACTCATTTGGTGG+GGG | - | Chr3:77999519-77999538 | None:intergenic | 35.0% |
| AACGTTAATACACACGTAGG+AGG | + | Chr3:77999999-78000018 | MsG0380016020.01.T01:CDS | 40.0% | |
| TCCGTGTTAAGTAAAGTCGT+CGG | - | Chr3:77999738-77999757 | None:intergenic | 40.0% | |
| TGAATTGTTGTACCATGGCA+CGG | - | Chr3:77999773-77999792 | None:intergenic | 40.0% | |
| TGTAAGGAGGGGTTTGATTA+GGG | - | Chr3:77999560-77999579 | None:intergenic | 40.0% | |
| ! | AGTAATGGAAGTGTAAGGAG+GGG | - | Chr3:77999571-77999590 | None:intergenic | 40.0% |
| ! | ATTAGGTGGTGATGATGATG+TGG | - | Chr3:77999829-77999848 | None:intergenic | 40.0% |
| ! | GGATTCATCATGGTTGATTC+AGG | - | Chr3:77999624-77999643 | None:intergenic | 40.0% |
| ! | GTAGTAATGGAAGTGTAAGG+AGG | - | Chr3:77999573-77999592 | None:intergenic | 40.0% |
| ! | GTTGTTGTTGAAGCTGTTGT+TGG | - | Chr3:77999932-77999951 | None:intergenic | 40.0% |
| ! | TGAAGCTGTTGTTGGTAGAA+AGG | - | Chr3:77999924-77999943 | None:intergenic | 40.0% |
| ! | TTCATCATGGTTGATTCAGG+GGG | - | Chr3:77999621-77999640 | None:intergenic | 40.0% |
| AGCCCATACGAAGAAGATCA+AGG | + | Chr3:77999697-77999716 | MsG0380016020.01.T01:CDS | 45.0% | |
| AGCCCTTGATCTTCTTCGTA+TGG | - | Chr3:77999703-77999722 | None:intergenic | 45.0% | |
| ATGGTACAACAATTCACCGG+AGG | + | Chr3:77999777-77999796 | MsG0380016020.01.T01:CDS | 45.0% | |
| CACTACTAATTTCCGTGCCA+TGG | + | Chr3:77999758-77999777 | MsG0380016020.01.T01:CDS | 45.0% | |
| CCTAATTTCTCAACCCTAGC+AGG | + | Chr3:77999843-77999862 | MsG0380016020.01.T01:CDS | 45.0% | |
| CCTGCTAGGGTTGAGAAATT+AGG | - | Chr3:77999846-77999865 | None:intergenic | 45.0% | |
| GCCATGGTACAACAATTCAC+CGG | + | Chr3:77999774-77999793 | MsG0380016020.01.T01:CDS | 45.0% | |
| GCCCATACGAAGAAGATCAA+GGG | + | Chr3:77999698-77999717 | MsG0380016020.01.T01:CDS | 45.0% | |
| GCCCTTGATCTTCTTCGTAT+GGG | - | Chr3:77999702-77999721 | None:intergenic | 45.0% | |
| GCCGACGACTTTACTTAACA+CGG | + | Chr3:77999734-77999753 | MsG0380016020.01.T01:CDS | 45.0% | |
| GCTAGGGTTGAGAAATTAGG+TGG | - | Chr3:77999843-77999862 | None:intergenic | 45.0% | |
| GGCAACGTTAATACACACGT+AGG | + | Chr3:77999996-78000015 | MsG0380016020.01.T01:CDS | 45.0% | |
| GTGTAAGGAGGGGTTTGATT+AGG | - | Chr3:77999561-77999580 | None:intergenic | 45.0% | |
| TCCGGTGAATTGTTGTACCA+TGG | - | Chr3:77999778-77999797 | None:intergenic | 45.0% | |
| TGGTGAGCTTGGATTCATCA+TGG | - | Chr3:77999634-77999653 | None:intergenic | 45.0% | |
| ! | AAAAGGTGCAATAGGACCTC+CGG | - | Chr3:77999796-77999815 | None:intergenic | 45.0% |
| ! | AGGGGTGGTGTTAGTAGTAA+TGG | - | Chr3:77999586-77999605 | None:intergenic | 45.0% |
| ! | TTCGTATGGGCTTTGACACA+CGG | - | Chr3:77999689-77999708 | None:intergenic | 45.0% |
| AGCAACTTGGGTCCATCTGA+AGG | + | Chr3:77999663-77999682 | MsG0380016020.01.T01:CDS | 50.0% | |
| AGGGGGTAAAGTTGAAGCAG+GGG | - | Chr3:77999604-77999623 | None:intergenic | 50.0% | |
| CAGGGGGTAAAGTTGAAGCA+GGG | - | Chr3:77999605-77999624 | None:intergenic | 50.0% | |
| GAAGAAGATCAAGGGCTTCG+CGG | + | Chr3:77999706-77999725 | MsG0380016020.01.T01:CDS | 50.0% | |
| TCAGGGGGTAAAGTTGAAGC+AGG | - | Chr3:77999606-77999625 | None:intergenic | 50.0% | |
| TTCAGATGGACCCAAGTTGC+TGG | - | Chr3:77999664-77999683 | None:intergenic | 50.0% | |
| TTTGACACACGGCCTTCAGA+TGG | - | Chr3:77999678-77999697 | None:intergenic | 50.0% | |
| ! | ATTTGGTGGGGGATCAGACA+TGG | - | Chr3:77999508-77999527 | None:intergenic | 50.0% |
| ! | GCTGCTGCAAAAGGTGCAAT+AGG | - | Chr3:77999804-77999823 | None:intergenic | 50.0% |
| ACCAACCTCAGCCAGCAACT+TGG | + | Chr3:77999650-77999669 | MsG0380016020.01.T01:CDS | 55.0% | |
| CCAACCTCAGCCAGCAACTT+GGG | + | Chr3:77999651-77999670 | MsG0380016020.01.T01:CDS | 55.0% | |
| GATGTGGCTGCTGCTGCAAA+AGG | - | Chr3:77999813-77999832 | None:intergenic | 55.0% | |
| GGGTAAAGTTGAAGCAGGGG+TGG | - | Chr3:77999601-77999620 | None:intergenic | 55.0% | |
| TCTCAACCCTAGCAGGCCTA+GGG | + | Chr3:77999850-77999869 | MsG0380016020.01.T01:CDS | 55.0% | |
| TTCTCAACCCTAGCAGGCCT+AGG | + | Chr3:77999849-77999868 | MsG0380016020.01.T01:CDS | 55.0% | |
| TCATAGGATGGGGAGCCCGT+GGG | - | Chr3:77999881-77999900 | None:intergenic | 60.0% | |
| TTCATAGGATGGGGAGCCCG+TGG | - | Chr3:77999882-77999901 | None:intergenic | 60.0% | |
| ! | CCCAAGTTGCTGGCTGAGGT+TGG | - | Chr3:77999654-77999673 | None:intergenic | 60.0% |
| ! | TGGACCCAAGTTGCTGGCTG+AGG | - | Chr3:77999658-77999677 | None:intergenic | 60.0% |
| !! | CTGGCTGAGGTTGGTGAGCT+TGG | - | Chr3:77999645-77999664 | None:intergenic | 60.0% |
| ACCCTAGCAGGCCTAGGGCT+TGG | + | Chr3:77999855-77999874 | MsG0380016020.01.T01:CDS | 65.0% | |
| GCCAAGCCCTAGGCCTGCTA+GGG | - | Chr3:77999859-77999878 | None:intergenic | 65.0% | |
| AGGCCTAGGGCTTGGCCCAC+GGG | + | Chr3:77999863-77999882 | MsG0380016020.01.T01:CDS | 70.0% | |
| CAGGCCTAGGGCTTGGCCCA+CGG | + | Chr3:77999862-77999881 | MsG0380016020.01.T01:CDS | 70.0% | |
| GGCCAAGCCCTAGGCCTGCT+AGG | - | Chr3:77999860-77999879 | None:intergenic | 70.0% | |
| GAGCCCGTGGGCCAAGCCCT+AGG | - | Chr3:77999869-77999888 | None:intergenic | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 77999495 | 78000049 | 77999495 | ID=MsG0380016020.01;Name=MsG0380016020.01 |
| Chr3 | mRNA | 77999495 | 78000049 | 77999495 | ID=MsG0380016020.01.T01;Parent=MsG0380016020.01;Name=MsG0380016020.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|0|1|0|184 |
| Chr3 | exon | 77999495 | 78000049 | 77999495 | ID=MsG0380016020.01.T01:exon:18374;Parent=MsG0380016020.01.T01 |
| Chr3 | CDS | 77999495 | 78000049 | 77999495 | ID=MsG0380016020.01.T01:cds;Parent=MsG0380016020.01.T01 |
| Gene Sequence |
| Protein sequence |
