Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880045272.01.T01 | XP_024627753.1 | 87.5 | 128 | 16 | 0 | 1 | 128 | 1 | 128 | 1.54E-59 | 190 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880045272.01.T01 | B7FH71 | 81.884 | 138 | 20 | 3 | 1 | 136 | 1 | 135 | 7.53e-57 | 182 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048312.01 | MsG0880045272.01 | 0.808827 | 2.557643e-50 | 1.477324e-47 |
| MsG0180000019.01 | MsG0880045272.01 | 0.839703 | 1.352014e-57 | 1.882246e-54 |
| MsG0180000337.01 | MsG0880045272.01 | 0.829284 | 5.610243e-55 | 5.717203e-52 |
| MsG0880044570.01 | MsG0880045272.01 | 0.833899 | 4.091100e-56 | 4.777478e-53 |
| MsG0880045272.01 | MsG0880045275.01 | 0.902737 | 7.140955e-79 | 1.025782e-74 |
| MsG0880045272.01 | MsG0880045277.01 | 0.871385 | 7.117115e-67 | 2.865508e-63 |
| MsG0880045272.01 | MsG0880045441.01 | 0.814458 | 1.524690e-51 | 1.023629e-48 |
| MsG0880045272.01 | MsG0880046176.01 | 0.875318 | 3.387451e-68 | 1.574502e-64 |
| MsG0880045272.01 | MsG0880046429.01 | 0.810844 | 9.417731e-51 | 5.738906e-48 |
| MsG0880045272.01 | MsG0880046996.01 | 0.840780 | 7.076363e-58 | 1.018747e-54 |
| MsG0880045272.01 | MsG0880047486.01 | 0.840841 | 6.820030e-58 | 9.836917e-55 |
| MsG0880045272.01 | MsG0880047491.01 | 0.812337 | 4.461165e-51 | 2.828901e-48 |
| MsG0180003830.01 | MsG0880045272.01 | 0.821534 | 3.837497e-53 | 3.130282e-50 |
| MsG0180004203.01 | MsG0880045272.01 | 0.833166 | 6.232905e-56 | 7.120064e-53 |
| MsG0180004266.01 | MsG0880045272.01 | 0.802256 | 6.114421e-49 | 2.981353e-46 |
| MsG0180004328.01 | MsG0880045272.01 | 0.808362 | 3.214325e-50 | 1.834347e-47 |
| MsG0180004562.01 | MsG0880045272.01 | 0.851037 | 1.152035e-60 | 2.301510e-57 |
| MsG0180004941.01 | MsG0880045272.01 | 0.809796 | 1.585017e-50 | 9.395322e-48 |
| MsG0180005328.01 | MsG0880045272.01 | 0.853860 | 1.807126e-61 | 3.960138e-58 |
| MsG0180005356.01 | MsG0880045272.01 | 0.809160 | 2.170255e-50 | 1.264842e-47 |
| MsG0180005357.01 | MsG0880045272.01 | 0.804449 | 2.149074e-49 | 1.108358e-46 |
| MsG0180005542.01 | MsG0880045272.01 | 0.815537 | 8.786072e-52 | 6.074154e-49 |
| MsG0780040806.01 | MsG0880045272.01 | 0.805269 | 1.448608e-49 | 7.630397e-47 |
| MsG0780041177.01 | MsG0880045272.01 | 0.827020 | 1.969962e-54 | 1.878691e-51 |
| MsG0780041316.01 | MsG0880045272.01 | 0.811323 | 7.415921e-51 | 4.576331e-48 |
| MsG0780041556.01 | MsG0880045272.01 | 0.828366 | 9.356736e-55 | 9.280595e-52 |
| MsG0880042740.01 | MsG0880045272.01 | 0.806262 | 8.961401e-50 | 4.842404e-47 |
| MsG0880043744.01 | MsG0880045272.01 | 0.843413 | 1.422929e-58 | 2.224674e-55 |
| MsG0880043828.01 | MsG0880045272.01 | 0.856648 | 2.790350e-62 | 6.706012e-59 |
| MsG0280011165.01 | MsG0880045272.01 | 0.838492 | 2.785327e-57 | 3.736728e-54 |
| MsG0280011193.01 | MsG0880045272.01 | 0.840004 | 1.128538e-57 | 1.586265e-54 |
| MsG0280011203.01 | MsG0880045272.01 | 0.848077 | 7.710458e-60 | 1.398360e-56 |
| MsG0380011613.01 | MsG0880045272.01 | 0.825132 | 5.538291e-54 | 5.004509e-51 |
| MsG0380012400.01 | MsG0880045272.01 | 0.832176 | 1.097299e-55 | 1.216973e-52 |
| MsG0380013272.01 | MsG0880045272.01 | 0.808390 | 3.170882e-50 | 1.810825e-47 |
| MsG0380013300.01 | MsG0880045272.01 | 0.840302 | 9.437957e-58 | 1.338637e-54 |
| MsG0680031240.01 | MsG0880045272.01 | 0.828554 | 8.428149e-55 | 8.405726e-52 |
| MsG0480019295.01 | MsG0880045272.01 | 0.834149 | 3.542473e-56 | 4.167055e-53 |
| MsG0480019304.01 | MsG0880045272.01 | 0.810139 | 1.337242e-50 | 7.997577e-48 |
| MsG0480020428.01 | MsG0880045272.01 | 0.802378 | 5.772187e-49 | 2.823337e-46 |
| MsG0480020497.01 | MsG0880045272.01 | 0.822977 | 1.774519e-53 | 1.508217e-50 |
| MsG0480021131.01 | MsG0880045272.01 | 0.811395 | 7.152283e-51 | 4.422389e-48 |
| MsG0480022125.01 | MsG0880045272.01 | 0.814496 | 1.495990e-51 | 1.005408e-48 |
| MsG0480022655.01 | MsG0880045272.01 | 0.847859 | 8.855021e-60 | 1.595166e-56 |
| MsG0480023107.01 | MsG0880045272.01 | 0.827066 | 1.921065e-54 | 1.834525e-51 |
| MsG0480023921.01 | MsG0880045272.01 | 0.823245 | 1.536849e-53 | 1.316052e-50 |
| MsG0580024398.01 | MsG0880045272.01 | 0.815799 | 7.681941e-52 | 5.349236e-49 |
| MsG0580024408.01 | MsG0880045272.01 | 0.800981 | 1.116630e-48 | 5.271830e-46 |
| MsG0380015010.01 | MsG0880045272.01 | 0.809496 | 1.838364e-50 | 1.081014e-47 |
| MsG0380016020.01 | MsG0880045272.01 | 0.814242 | 1.701834e-51 | 1.135897e-48 |
| MsG0380016839.01 | MsG0880045272.01 | 0.852694 | 3.901376e-61 | 8.219815e-58 |
| MsG0380017550.01 | MsG0880045272.01 | 0.802931 | 4.439568e-49 | 2.202151e-46 |
| MsG0380018051.01 | MsG0880045272.01 | 0.816757 | 4.690001e-52 | 3.352468e-49 |
| MsG0580027646.01 | MsG0880045272.01 | 0.802685 | 4.990062e-49 | 2.459771e-46 |
| MsG0580027733.01 | MsG0880045272.01 | 0.806781 | 6.961093e-50 | 3.811622e-47 |
| MsG0580027797.01 | MsG0880045272.01 | 0.811281 | 7.572570e-51 | 4.667871e-48 |
| MsG0580028999.01 | MsG0880045272.01 | 0.868861 | 4.767272e-66 | 1.751386e-62 |
| MsG0580029626.01 | MsG0880045272.01 | 0.837246 | 5.820752e-57 | 7.515830e-54 |
| MsG0580030133.01 | MsG0880045272.01 | 0.803196 | 3.913058e-49 | 1.954218e-46 |
| MsG0280006883.01 | MsG0880045272.01 | 0.821812 | 3.308025e-53 | 2.720008e-50 |
| MsG0280008405.01 | MsG0880045272.01 | 0.847305 | 1.257726e-59 | 2.225789e-56 |
| MsG0280009394.01 | MsG0880045272.01 | 0.865257 | 6.733682e-65 | 2.176685e-61 |
| MsG0780036661.01 | MsG0880045272.01 | 0.813276 | 2.778851e-51 | 1.806682e-48 |
| MsG0780038755.01 | MsG0880045272.01 | 0.807956 | 3.924140e-50 | 2.215421e-47 |
| MsG0780039783.01 | MsG0880045272.01 | 0.813996 | 1.928840e-51 | 1.278789e-48 |
| MsG0780039840.01 | MsG0880045272.01 | 0.834990 | 2.177089e-56 | 2.626754e-53 |
| MsG0780039877.01 | MsG0880045272.01 | 0.848357 | 6.454442e-60 | 1.181361e-56 |
| MsG0780039978.01 | MsG0880045272.01 | 0.844155 | 9.004275e-59 | 1.440878e-55 |
| MsG0780040041.01 | MsG0880045272.01 | 0.812820 | 3.498214e-51 | 2.246495e-48 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 41 sgRNAs with CRISPR-Local
Find 46 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGCCCACCTGGGCCTGATGC+TGG | 0.216285 | 8:-56758193 | MsG0880045272.01.T01:CDS |
| GGGGAAGATGTGTTTGGGCT+TGG | 0.239614 | 8:-56758148 | MsG0880045272.01.T01:CDS |
| GGGAAGATGTGTTTGGGCTT+GGG | 0.240843 | 8:-56758147 | MsG0880045272.01.T01:CDS |
| GTTTGTGATATTAGGTCTTT+TGG | 0.255388 | 8:+56757985 | None:intergenic |
| TGCTCTAGTCCGGGCCCACC+TGG | 0.276732 | 8:-56758205 | MsG0880045272.01.T01:CDS |
| ATCAAATTGTTTGTGATATT+AGG | 0.276758 | 8:+56757977 | None:intergenic |
| GCTGGTGGGGAAGATGTGTT+TGG | 0.322107 | 8:-56758154 | MsG0880045272.01.T01:CDS |
| GATGGAGCTGGTGCTGAATT+TGG | 0.324202 | 8:-56758061 | MsG0880045272.01.T01:CDS |
| AGCTCCTGGCTGCTCTAGTC+CGG | 0.331077 | 8:-56758215 | MsG0880045272.01.T01:CDS |
| TGCTGGAATCTTTGTGTCCA+TGG | 0.342466 | 8:+56758294 | None:intergenic |
| GATGTGTTTGGGCTTGGGCC+TGG | 0.365493 | 8:-56758142 | MsG0880045272.01.T01:CDS |
| CCTTCAACTTCTTCTTCTCC+AGG | 0.374999 | 8:+56758124 | None:intergenic |
| GCCCACCTGGGCCTGATGCT+GGG | 0.385695 | 8:-56758192 | MsG0880045272.01.T01:CDS |
| GCAGAGGGTGACTCAGCTCC+TGG | 0.403105 | 8:-56758229 | MsG0880045272.01.T01:CDS |
| CTATTAGAGCCTGGTGCTGG+TGG | 0.429246 | 8:-56758169 | MsG0880045272.01.T01:CDS |
| GAAGTTGAAGGAGATATTGC+AGG | 0.434197 | 8:-56758112 | MsG0880045272.01.T01:CDS |
| CTGGTGGGGAAGATGTGTTT+GGG | 0.437212 | 8:-56758153 | MsG0880045272.01.T01:CDS |
| TGAAGGAGATATTGCAGGAG+AGG | 0.441071 | 8:-56758107 | MsG0880045272.01.T01:CDS |
| GATATTGCAGGAGAGGTTGT+AGG | 0.444704 | 8:-56758100 | MsG0880045272.01.T01:CDS |
| TATTAGAGCCTGGTGCTGGT+GGG | 0.447878 | 8:-56758168 | MsG0880045272.01.T01:CDS |
| GGCTCTAATAGCCCAGCATC+AGG | 0.456286 | 8:+56758181 | None:intergenic |
| GCATCAGGCCCAGGTGGGCC+CGG | 0.462450 | 8:+56758196 | None:intergenic |
| GCTCCTGGCTGCTCTAGTCC+GGG | 0.489743 | 8:-56758214 | MsG0880045272.01.T01:CDS |
| GGGCTATTAGAGCCTGGTGC+TGG | 0.490149 | 8:-56758172 | MsG0880045272.01.T01:CDS |
| ATTAGAGCCTGGTGCTGGTG+GGG | 0.496717 | 8:-56758167 | MsG0880045272.01.T01:CDS |
| GCTCTAGTCCGGGCCCACCT+GGG | 0.506944 | 8:-56758204 | MsG0880045272.01.T01:CDS |
| GATGCTGGGCTATTAGAGCC+TGG | 0.508970 | 8:-56758178 | MsG0880045272.01.T01:CDS |
| GATTGTGTGGGTGATGGTGC+AGG | 0.539483 | 8:-56758031 | MsG0880045272.01.T01:CDS |
| CCTGGAGAAGAAGAAGTTGA+AGG | 0.541963 | 8:-56758124 | MsG0880045272.01.T01:CDS |
| GATAATGATTGTGTGGGTGA+TGG | 0.545354 | 8:-56758037 | MsG0880045272.01.T01:CDS |
| AATAGCCCAGCATCAGGCCC+AGG | 0.552354 | 8:+56758187 | None:intergenic |
| AGCCCAGCATCAGGCCCAGG+TGG | 0.566917 | 8:+56758190 | None:intergenic |
| GGGCCCGGACTAGAGCAGCC+AGG | 0.581693 | 8:+56758211 | None:intergenic |
| TTTGTGGAAGGTGATGGAGC+TGG | 0.593413 | 8:-56758073 | MsG0880045272.01.T01:CDS |
| ATTTGGTGATAATGATTGTG+TGG | 0.597910 | 8:-56758044 | MsG0880045272.01.T01:CDS |
| AGTAACAGCAACAAAAGTGT+TGG | 0.609923 | 8:-56758266 | MsG0880045272.01.T01:CDS |
| ACATCTTCCCCACCAGCACC+AGG | 0.610370 | 8:+56758160 | None:intergenic |
| GTGATAGAGAATGCAGCAGA+GGG | 0.615284 | 8:-56758244 | MsG0880045272.01.T01:CDS |
| GCCCAGCATCAGGCCCAGGT+GGG | 0.625332 | 8:+56758191 | None:intergenic |
| TTTGGTGATAATGATTGTGT+GGG | 0.641938 | 8:-56758043 | MsG0880045272.01.T01:CDS |
| GGTGATAGAGAATGCAGCAG+AGG | 0.711837 | 8:-56758245 | MsG0880045272.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TCAAAAAATATATACAACAT+GGG | + | Chr8:56758255-56758274 | None:intergenic | 15.0% |
| !! | ATCAAATTGTTTGTGATATT+AGG | + | Chr8:56758222-56758241 | None:intergenic | 20.0% |
| !! | CTCAAAAAATATATACAACA+TGG | + | Chr8:56758256-56758275 | None:intergenic | 20.0% |
| ! | ATTTGGTGATAATGATTGTG+TGG | - | Chr8:56758152-56758171 | MsG0880045272.01.T01:CDS | 30.0% |
| ! | GTTTGTGATATTAGGTCTTT+TGG | + | Chr8:56758214-56758233 | None:intergenic | 30.0% |
| ! | TTTGGTGATAATGATTGTGT+GGG | - | Chr8:56758153-56758172 | MsG0880045272.01.T01:CDS | 30.0% |
| ! | AGTAACAGCAACAAAAGTGT+TGG | - | Chr8:56757930-56757949 | MsG0880045272.01.T01:CDS | 35.0% |
| GAAGTTGAAGGAGATATTGC+AGG | - | Chr8:56758084-56758103 | MsG0880045272.01.T01:CDS | 40.0% | |
| GATAATGATTGTGTGGGTGA+TGG | - | Chr8:56758159-56758178 | MsG0880045272.01.T01:CDS | 40.0% | |
| ! | AGAGGTTGTAGGTGATTTTG+TGG | - | Chr8:56758107-56758126 | MsG0880045272.01.T01:CDS | 40.0% |
| ! | GTTGTAGGTGATTTTGTGGA+AGG | - | Chr8:56758111-56758130 | MsG0880045272.01.T01:CDS | 40.0% |
| CCTGGAGAAGAAGAAGTTGA+AGG | - | Chr8:56758072-56758091 | MsG0880045272.01.T01:CDS | 45.0% | |
| CCTTCAACTTCTTCTTCTCC+AGG | + | Chr8:56758075-56758094 | None:intergenic | 45.0% | |
| GATATTGCAGGAGAGGTTGT+AGG | - | Chr8:56758096-56758115 | MsG0880045272.01.T01:CDS | 45.0% | |
| GTGATAGAGAATGCAGCAGA+GGG | - | Chr8:56757952-56757971 | MsG0880045272.01.T01:CDS | 45.0% | |
| TGAAGGAGATATTGCAGGAG+AGG | - | Chr8:56758089-56758108 | MsG0880045272.01.T01:CDS | 45.0% | |
| ! | GGTGATTTTGTGGAAGGTGA+TGG | - | Chr8:56758117-56758136 | MsG0880045272.01.T01:CDS | 45.0% |
| !! | GTTGCTGTTACTGCTTTTGC+TGG | + | Chr8:56757922-56757941 | None:intergenic | 45.0% |
| CTGGTGGGGAAGATGTGTTT+GGG | - | Chr8:56758043-56758062 | MsG0880045272.01.T01:CDS | 50.0% | |
| GGGAAGATGTGTTTGGGCTT+GGG | - | Chr8:56758049-56758068 | MsG0880045272.01.T01:CDS | 50.0% | |
| GGTGATAGAGAATGCAGCAG+AGG | - | Chr8:56757951-56757970 | MsG0880045272.01.T01:CDS | 50.0% | |
| ! | GATGGAGCTGGTGCTGAATT+TGG | - | Chr8:56758135-56758154 | MsG0880045272.01.T01:CDS | 50.0% |
| !! | TATTAGAGCCTGGTGCTGGT+GGG | - | Chr8:56758028-56758047 | MsG0880045272.01.T01:CDS | 50.0% |
| !! | TTTGTGGAAGGTGATGGAGC+TGG | - | Chr8:56758123-56758142 | MsG0880045272.01.T01:CDS | 50.0% |
| GATGCTGGGCTATTAGAGCC+TGG | - | Chr8:56758018-56758037 | MsG0880045272.01.T01:CDS | 55.0% | |
| GCTGGTGGGGAAGATGTGTT+TGG | - | Chr8:56758042-56758061 | MsG0880045272.01.T01:CDS | 55.0% | |
| GGGGAAGATGTGTTTGGGCT+TGG | - | Chr8:56758048-56758067 | MsG0880045272.01.T01:CDS | 55.0% | |
| ! | CTATTAGAGCCTGGTGCTGG+TGG | - | Chr8:56758027-56758046 | MsG0880045272.01.T01:CDS | 55.0% |
| ! | GATTGTGTGGGTGATGGTGC+AGG | - | Chr8:56758165-56758184 | MsG0880045272.01.T01:CDS | 55.0% |
| ! | GGCTCTAATAGCCCAGCATC+AGG | + | Chr8:56758018-56758037 | None:intergenic | 55.0% |
| !! | ATTAGAGCCTGGTGCTGGTG+GGG | - | Chr8:56758029-56758048 | MsG0880045272.01.T01:CDS | 55.0% |
| AATAGCCCAGCATCAGGCCC+AGG | + | Chr8:56758012-56758031 | None:intergenic | 60.0% | |
| ACATCTTCCCCACCAGCACC+AGG | + | Chr8:56758039-56758058 | None:intergenic | 60.0% | |
| AGCTCCTGGCTGCTCTAGTC+CGG | - | Chr8:56757981-56758000 | MsG0880045272.01.T01:CDS | 60.0% | |
| GATGTGTTTGGGCTTGGGCC+TGG | - | Chr8:56758054-56758073 | MsG0880045272.01.T01:CDS | 60.0% | |
| GGGCTATTAGAGCCTGGTGC+TGG | - | Chr8:56758024-56758043 | MsG0880045272.01.T01:CDS | 60.0% | |
| GCAGAGGGTGACTCAGCTCC+TGG | - | Chr8:56757967-56757986 | MsG0880045272.01.T01:CDS | 65.0% | |
| GCTCCTGGCTGCTCTAGTCC+GGG | - | Chr8:56757982-56758001 | MsG0880045272.01.T01:CDS | 65.0% | |
| AGCCCAGCATCAGGCCCAGG+TGG | + | Chr8:56758009-56758028 | None:intergenic | 70.0% | |
| GCCCACCTGGGCCTGATGCT+GGG | - | Chr8:56758004-56758023 | MsG0880045272.01.T01:CDS | 70.0% | |
| GCCCAGCATCAGGCCCAGGT+GGG | + | Chr8:56758008-56758027 | None:intergenic | 70.0% | |
| GCTCTAGTCCGGGCCCACCT+GGG | - | Chr8:56757992-56758011 | MsG0880045272.01.T01:CDS | 70.0% | |
| TGCTCTAGTCCGGGCCCACC+TGG | - | Chr8:56757991-56758010 | MsG0880045272.01.T01:CDS | 70.0% | |
| GCATCAGGCCCAGGTGGGCC+CGG | + | Chr8:56758003-56758022 | None:intergenic | 75.0% | |
| GGCCCACCTGGGCCTGATGC+TGG | - | Chr8:56758003-56758022 | MsG0880045272.01.T01:CDS | 75.0% | |
| ! | GGGCCCGGACTAGAGCAGCC+AGG | + | Chr8:56757988-56758007 | None:intergenic | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 56757904 | 56758314 | 56757904 | ID=MsG0880045272.01;Name=MsG0880045272.01 |
| Chr8 | mRNA | 56757904 | 56758314 | 56757904 | ID=MsG0880045272.01.T01;Parent=MsG0880045272.01;Name=MsG0880045272.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|136 |
| Chr8 | exon | 56757904 | 56758314 | 56757904 | ID=MsG0880045272.01.T01:exon:1172;Parent=MsG0880045272.01.T01 |
| Chr8 | CDS | 56757904 | 56758314 | 56757904 | ID=MsG0880045272.01.T01:cds;Parent=MsG0880045272.01.T01 |
| Gene Sequence |
| Protein sequence |
