AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1427560


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1427560 MtrunA17_Chr2g0297101 100.000 135 0 0 1 135 1 135 4.49e-95 270
Msa1427560 MtrunA17_Chr2g0277391 99.259 135 1 0 1 135 1 135 1.53e-94 268
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1427560 AT4G15000.1 86.667 135 18 0 1 135 1 135 7.89e-84 241
Msa1427560 AT3G22230.1 86.667 135 18 0 1 135 1 135 1.24e-83 241
Msa1427560 AT2G32220.1 80.000 135 27 0 1 135 1 135 7.77e-78 226
Msa1427560 AT4G15000.2 85.366 123 18 0 1 123 1 123 4.27e-73 214

Find 34 sgRNAs with CRISPR-Local

Find 34 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACGATGACGGCTTTCTTCC+CGG 0.266318 tig0026191:-55518 None:intergenic
TACGGGCATTGTCTTGTTGC+AGG 0.269826 tig0026191:+55574 Msa1427560:CDS
TCCCGGCGTAGCGTCCTTGT+AGG 0.400507 tig0026191:-55501 None:intergenic
GGTTTAAGACTGGGAAGAAT+AGG 0.401208 tig0026191:+55821 Msa1427560:CDS
GGAACTCGCGACCGTGCTTA+CGG 0.402067 tig0026191:+55556 Msa1427560:CDS
AGGACGATGACGGCTTTGTT+TGG 0.406607 tig0026191:-55481 None:intergenic
TACTGATGTTCTTCAGAGTA+AGG 0.417538 tig0026191:+55750 Msa1427560:CDS
TAAGAAGGTGACTGCTTTGA+AGG 0.432710 tig0026191:+55774 Msa1427560:CDS
TCCTTCTTGATAACCTTAGC+TGG 0.439811 tig0026191:-55610 None:intergenic
CTTCACACGCGATTTCTTCG+CGG 0.451730 tig0026191:-55647 None:intergenic
GAACTCGCGACCGTGCTTAC+GGG 0.459112 tig0026191:+55557 Msa1427560:CDS
GGTGGTTCTTTACCAAGCTC+AGG 0.462687 tig0026191:+55842 Msa1427560:CDS
GTCGAAATTCTTCACGATGA+CGG 0.503904 tig0026191:-55530 None:intergenic
TTCGAGGAGAGGTTTAAGAC+TGG 0.506310 tig0026191:+55811 Msa1427560:CDS
ACGGGCATTGTCTTGTTGCA+GGG 0.511441 tig0026191:+55575 Msa1427560:CDS
CGCGAAGAAATCGCGTGTGA+AGG 0.529778 tig0026191:+55648 Msa1427560:CDS
AACGAGTCGGCATCAGATGC+TGG 0.530504 tig0026191:-55693 None:intergenic
CGAAGAAGAGGTTCGAGGAG+AGG 0.532977 tig0026191:+55800 Msa1427560:CDS
TCCAGCTAAGGTTATCAAGA+AGG 0.538513 tig0026191:+55609 Msa1427560:CDS
TCGAGGAGAGGTTTAAGACT+GGG 0.540939 tig0026191:+55812 Msa1427560:CDS
AACAGCTCAAAACCTGAGCT+TGG 0.543643 tig0026191:-55854 None:intergenic
AAAGCCGTCATCGTCCTACA+AGG 0.551105 tig0026191:+55487 Msa1427560:CDS
TCTTGATGTTGATTTGAAAG+AGG 0.559046 tig0026191:+55720 Msa1427560:CDS
TCTTCAGAGTAAGGATAAGA+AGG 0.568239 tig0026191:+55759 Msa1427560:CDS
GTCCTACAAGGACGCTACGC+CGG 0.584899 tig0026191:+55499 Msa1427560:CDS
CAAGACAATGCCCGTAAGCA+CGG 0.587108 tig0026191:-55567 None:intergenic
CTTTGAAGGAGGCGAAGAAG+AGG 0.602084 tig0026191:+55788 Msa1427560:CDS
GATCAAGAAGTATCCAGCTA+AGG 0.602579 tig0026191:+55597 Msa1427560:CDS
GGAGGCGAAGAAGAGGTTCG+AGG 0.607573 tig0026191:+55795 Msa1427560:CDS
TTAAGACTGGGAAGAATAGG+TGG 0.614047 tig0026191:+55824 Msa1427560:CDS
GCGTCCTTGTAGGACGATGA+CGG 0.630553 tig0026191:-55491 None:intergenic
GAAGGTGACTGCTTTGAAGG+AGG 0.640915 tig0026191:+55777 Msa1427560:CDS
ACATCAAGAGTGTAACGAGT+CGG 0.653533 tig0026191:-55706 None:intergenic
ATCGTGAAGAATTTCGACGA+CGG 0.729202 tig0026191:+55535 Msa1427560:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TCTTGATGTTGATTTGAAAG+AGG + tig0026191:55720-55739 Msa1427560:CDS 30.0%
TACTGATGTTCTTCAGAGTA+AGG + tig0026191:55750-55769 Msa1427560:CDS 35.0%
TCTTCAGAGTAAGGATAAGA+AGG + tig0026191:55759-55778 Msa1427560:CDS 35.0%
GTCGAAATTCTTCACGATGA+CGG - tig0026191:55533-55552 None:intergenic 40.0%
ATCGTGAAGAATTTCGACGA+CGG + tig0026191:55535-55554 Msa1427560:CDS 40.0%
!! GATCAAGAAGTATCCAGCTA+AGG + tig0026191:55597-55616 Msa1427560:CDS 40.0%
TCCAGCTAAGGTTATCAAGA+AGG + tig0026191:55609-55628 Msa1427560:CDS 40.0%
! TCCTTCTTGATAACCTTAGC+TGG - tig0026191:55613-55632 None:intergenic 40.0%
! ACATCAAGAGTGTAACGAGT+CGG - tig0026191:55709-55728 None:intergenic 40.0%
TAAGAAGGTGACTGCTTTGA+AGG + tig0026191:55774-55793 Msa1427560:CDS 40.0%
! GGTTTAAGACTGGGAAGAAT+AGG + tig0026191:55821-55840 Msa1427560:CDS 40.0%
TTAAGACTGGGAAGAATAGG+TGG + tig0026191:55824-55843 Msa1427560:CDS 40.0%
! TTCGAGGAGAGGTTTAAGAC+TGG + tig0026191:55811-55830 Msa1427560:CDS 45.0%
! TCGAGGAGAGGTTTAAGACT+GGG + tig0026191:55812-55831 Msa1427560:CDS 45.0%
!! AGGACGATGACGGCTTTGTT+TGG - tig0026191:55484-55503 None:intergenic 50.0%
AAAGCCGTCATCGTCCTACA+AGG + tig0026191:55487-55506 Msa1427560:CDS 50.0%
CAAGACAATGCCCGTAAGCA+CGG - tig0026191:55570-55589 None:intergenic 50.0%
! TACGGGCATTGTCTTGTTGC+AGG + tig0026191:55574-55593 Msa1427560:CDS 50.0%
! ACGGGCATTGTCTTGTTGCA+GGG + tig0026191:55575-55594 Msa1427560:CDS 50.0%
CTTCACACGCGATTTCTTCG+CGG - tig0026191:55650-55669 None:intergenic 50.0%
!! GAAGGTGACTGCTTTGAAGG+AGG + tig0026191:55777-55796 Msa1427560:CDS 50.0%
! CTTTGAAGGAGGCGAAGAAG+AGG + tig0026191:55788-55807 Msa1427560:CDS 50.0%
GGTGGTTCTTTACCAAGCTC+AGG + tig0026191:55842-55861 Msa1427560:CDS 50.0%
GCGTCCTTGTAGGACGATGA+CGG - tig0026191:55494-55513 None:intergenic 55.0%
! CACGATGACGGCTTTCTTCC+CGG - tig0026191:55521-55540 None:intergenic 55.0%
CGCGAAGAAATCGCGTGTGA+AGG + tig0026191:55648-55667 Msa1427560:CDS 55.0%
AACGAGTCGGCATCAGATGC+TGG - tig0026191:55696-55715 None:intergenic 55.0%
CGAAGAAGAGGTTCGAGGAG+AGG + tig0026191:55800-55819 Msa1427560:CDS 55.0%
GTCCTACAAGGACGCTACGC+CGG + tig0026191:55499-55518 Msa1427560:CDS 60.0%
TCCTACAAGGACGCTACGCC+GGG + tig0026191:55500-55519 Msa1427560:CDS 60.0%
GGAACTCGCGACCGTGCTTA+CGG + tig0026191:55556-55575 Msa1427560:CDS 60.0%
GAACTCGCGACCGTGCTTAC+GGG + tig0026191:55557-55576 Msa1427560:CDS 60.0%
GGAGGCGAAGAAGAGGTTCG+AGG + tig0026191:55795-55814 Msa1427560:CDS 60.0%
TCCCGGCGTAGCGTCCTTGT+AGG - tig0026191:55504-55523 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
tig0026191 gene 55463 55870 55463 ID=Msa1427560;Name=Msa1427560
tig0026191 mRNA 55463 55870 55463 ID=Msa1427560-mRNA-1;Parent=Msa1427560;Name=Msa1427560-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|135
tig0026191 exon 55463 55870 55463 ID=Msa1427560-mRNA-1:exon:5485;Parent=Msa1427560-mRNA-1
tig0026191 CDS 55463 55870 55463 ID=Msa1427560-mRNA-1:cds;Parent=Msa1427560-mRNA-1
Gene Sequence

>Msa1427560

ATGGTGAAATTCTTGAAGCCAAACAAAGCCGTCATCGTCCTACAAGGACGCTACGCCGGGAAGAAAGCCGTCATCGTGAAGAATTTCGACGACGGAACTCGCGACCGTGCTTACGGGCATTGTCTTGTTGCAGGGATCAAGAAGTATCCAGCTAAGGTTATCAAGAAGGATTCAGCGAAGAAAACCGCGAAGAAATCGCGTGTGAAGGCTTTCGTGAAGCTTGTTAACTACCAGCATCTGATGCCGACTCGTTACACTCTTGATGTTGATTTGAAAGAGGTTGTTACTACTGATGTTCTTCAGAGTAAGGATAAGAAGGTGACTGCTTTGAAGGAGGCGAAGAAGAGGTTCGAGGAGAGGTTTAAGACTGGGAAGAATAGGTGGTTCTTTACCAAGCTCAGGTTTTGA

Protein sequence

>Msa1427560

MVKFLKPNKAVIVLQGRYAGKKAVIVKNFDDGTRDRAYGHCLVAGIKKYPAKVIKKDSAKKTAKKSRVKAFVKLVNYQHLMPTRYTLDVDLKEVVTTDVLQSKDKKVTALKEAKKRFEERFKTGKNRWFFTKLRF*