AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0090580

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0090580 MtrunA17_Chr2g0291901 100.000 85 0 0 15 99 2 86 8.33e-60 177
Msa0090580 MtrunA17_Chr1g0210581 100.000 85 0 0 15 99 2 86 8.33e-60 177
Msa0090580 MtrunA17_Chr2g0283551 98.824 85 1 0 15 99 2 86 1.90e-59 176
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0090580 AT3G61110.1 96.471 85 3 0 15 99 2 86 1.12e-58 175
Msa0090580 AT2G45710.1 95.181 83 4 0 15 97 2 84 1.05e-55 167
Msa0090580 AT5G47930.1 96.386 83 3 0 15 97 2 84 2.85e-55 166
Msa0090580 AT3G61111.1 83.529 85 14 0 15 99 2 86 1.35e-48 149
Msa0090580 AT3G61111.2 80.488 41 8 0 15 55 2 42 1.11e-17 71.2

Find 43 sgRNAs with CRISPR-Local

Find 200 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATTATTTAATGAGAAATTT+TGG 0.186968 1_2:+72659994 Msa0090580:three_prime_UTR
TTGCTTTGCCGCCGGTTGGC+TGG 0.271725 1_2:-72659884 None:intergenic
TCCACAGCCCTCTCAACTTT+GGG 0.311830 1_2:-72658631 None:intergenic
TAACAGAGGGGTGCTCCTTC+AGG 0.320285 1_2:+72659910 Msa0090580:CDS
ATCTCCTAACTCATTCTTCA+TGG 0.338607 1_2:+72659041 Msa0090580:CDS
TCAAGAGATCGATGTCGTTT+TGG 0.351397 1_2:-72658966 None:intergenic
TGAGTAAATTTGGTTTGATA+TGG 0.363323 1_2:+72660099 Msa0090580:three_prime_UTR
TTCCACAGCCCTCTCAACTT+TGG 0.365114 1_2:-72658632 None:intergenic
CACTAGTCATGCTAAATTAG+AGG 0.384775 1_2:-72660260 None:intergenic
GGAAGAAGGGTGATTAGTTA+TGG 0.386772 1_2:+72659931 Msa0090580:exon
GCATGACTAGTGTACTTGTT+TGG 0.418818 1_2:+72660271 Msa0090580:three_prime_UTR
AATACCATGAAGAATGAGTT+AGG 0.426658 1_2:-72659045 None:intergenic
AAATTTAATTTAGTGCTAAC+GGG 0.427882 1_2:-72660136 None:intergenic
CTTACATGTTGAAGCATCCT+TGG 0.445045 1_2:-72659176 None:intergenic
AGCCAACCGGCGGCAAAGCA+AGG 0.463038 1_2:+72659886 Msa0090580:CDS
ACACATTAACACAAGACAAC+TGG 0.469557 1_2:-72660047 None:intergenic
GGTATGGCTACATGTGTCAT+AGG 0.470144 1_2:+72660292 Msa0090580:three_prime_UTR
ACGGTATTGTGCCAGCCAAC+CGG 0.474939 1_2:+72659873 Msa0090580:CDS
AGCCTTGCTTTGCCGCCGGT+TGG 0.478403 1_2:-72659888 None:intergenic
TAAATTTAATTTAGTGCTAA+CGG 0.479815 1_2:-72660137 None:intergenic
CACCAGCGGAGCTTGAGAAA+AGG 0.496318 1_2:+72658992 Msa0090580:CDS
GGGGTGCTCCTTCAGGAAGA+AGG 0.503456 1_2:+72659917 Msa0090580:CDS
TCTCAAACAGTCGTGGTTTG+CGG 0.511373 1_2:+72659840 Msa0090580:CDS
TAAATTTGGTTTGATATGGT+TGG 0.518219 1_2:+72660103 Msa0090580:three_prime_UTR
TGTTAGCCTTGCTTTGCCGC+CGG 0.526081 1_2:-72659892 None:intergenic
ACTAGTGTACTTGTTTGGTA+TGG 0.527204 1_2:+72660276 Msa0090580:three_prime_UTR
TTATAGGATGTCAAATGCCA+AGG 0.569104 1_2:+72659159 Msa0090580:intron
GGGTGCTCCTTCAGGAAGAA+GGG 0.579020 1_2:+72659918 Msa0090580:CDS
GGCAAAGCAAGGCTAACAGA+GGG 0.582316 1_2:+72659897 Msa0090580:CDS
GATGGGACCCAAAGTTGAGA+GGG 0.597213 1_2:+72658624 None:intergenic
ACCCAAAGTTGAGAGGGCTG+TGG 0.599911 1_2:+72658630 Msa0090580:CDS
ACTAGTCATGCTAAATTAGA+GGG 0.602198 1_2:-72660259 None:intergenic
CTAATCACCCTTCTTCCTGA+AGG 0.602303 1_2:-72659925 None:intergenic
AGATGGGACCCAAAGTTGAG+AGG 0.608186 1_2:+72658623 None:intergenic
TAGCCACTCTCAAACAGTCG+TGG 0.620995 1_2:+72659833 Msa0090580:CDS
GGCTGTGGAATCAAATGTGG+AGG 0.628873 1_2:+72658645 Msa0090580:CDS
CGGCAAAGCAAGGCTAACAG+AGG 0.648379 1_2:+72659896 Msa0090580:CDS
GGTTTGCGGCAACTGTCAGA+CGG 0.650064 1_2:+72659854 Msa0090580:CDS
AAACCACGACTGTTTGAGAG+TGG 0.670924 1_2:-72659836 None:intergenic
GAGGGCTGTGGAATCAAATG+TGG 0.673501 1_2:+72658642 Msa0090580:CDS
CGATCTCTTGAACCCACCAG+CGG 0.674494 1_2:+72658978 Msa0090580:CDS
GCAAAGCAAGGCTAACAGAG+GGG 0.682022 1_2:+72659898 Msa0090580:CDS
GTATTGTGCCAGCCAACCGG+CGG 0.694777 1_2:+72659876 Msa0090580:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAAAACAAATTACAAA+CGG - chr1_2:72659117-72659136 None:intergenic 10.0%
!!! TTATTATATTTCTATCATTT+AGG + chr1_2:72659534-72659553 Msa0090580:intron 10.0%
!!! TTTTTTTTTGTTTTTTTTGA+AGG + chr1_2:72660175-72660194 Msa0090580:intron 10.0%
!! AAAAAAAAACAAATTACAAA+CGG - chr1_2:72659117-72659136 None:intergenic 10.0%
!!! TTATTATATTTCTATCATTT+AGG + chr1_2:72659534-72659553 Msa0090580:intron 10.0%
!!! TTTTTTTTTGTTTTTTTTGA+AGG + chr1_2:72660175-72660194 Msa0090580:intron 10.0%
!! TAAATGGAAAATCTATTTAA+AGG - chr1_2:72658748-72658767 None:intergenic 15.0%
!! AATTAACATATACATAACAA+TGG - chr1_2:72659086-72659105 None:intergenic 15.0%
!!! AAATTACTTTTTTATTCAAC+CGG - chr1_2:72659271-72659290 None:intergenic 15.0%
!! TAATCTATCATTTAAATTGA+TGG + chr1_2:72659446-72659465 Msa0090580:intron 15.0%
!! TTTATAATTGTTGAAATTTG+TGG + chr1_2:72659792-72659811 Msa0090580:intron 15.0%
!!! GATTATTTAATGAGAAATTT+TGG + chr1_2:72659994-72660013 Msa0090580:three_prime_UTR 15.0%
!!! TAAATTTAATTTAGTGCTAA+CGG - chr1_2:72660140-72660159 None:intergenic 15.0%
!! TAAATGGAAAATCTATTTAA+AGG - chr1_2:72658748-72658767 None:intergenic 15.0%
!! AATTAACATATACATAACAA+TGG - chr1_2:72659086-72659105 None:intergenic 15.0%
!!! AAATTACTTTTTTATTCAAC+CGG - chr1_2:72659271-72659290 None:intergenic 15.0%
!! TAATCTATCATTTAAATTGA+TGG + chr1_2:72659446-72659465 Msa0090580:intron 15.0%
!! TTTATAATTGTTGAAATTTG+TGG + chr1_2:72659792-72659811 Msa0090580:intron 15.0%
!!! GATTATTTAATGAGAAATTT+TGG + chr1_2:72659994-72660013 Msa0090580:three_prime_UTR 15.0%
!!! TAAATTTAATTTAGTGCTAA+CGG - chr1_2:72660140-72660159 None:intergenic 15.0%
!!! TATATCATATCTTTTTCACA+TGG + chr1_2:72658691-72658710 Msa0090580:intron 20.0%
!!! ATATCATATCTTTTTCACAT+GGG + chr1_2:72658692-72658711 Msa0090580:intron 20.0%
!! AAAAGACTGTAAATGTTAAA+TGG - chr1_2:72658764-72658783 None:intergenic 20.0%
!! CAAACTTAATCAATCATAAT+TGG - chr1_2:72658922-72658941 None:intergenic 20.0%
!!! TTTCTCTTTGTATTTGTTAT+AGG + chr1_2:72659143-72659162 Msa0090580:intron 20.0%
!!! AATTACTTTTTTATTCAACC+GGG - chr1_2:72659270-72659289 None:intergenic 20.0%
!! GGTTTATTCAACTAAAAAAA+TGG - chr1_2:72659332-72659351 None:intergenic 20.0%
!! TAGTTCAATGACTAAATTTA+AGG - chr1_2:72659353-72659372 None:intergenic 20.0%
!! TTATTATACTTATACAACTC+AGG - chr1_2:72659396-72659415 None:intergenic 20.0%
!! TATAAGTATAATAAACAGTC+CGG + chr1_2:72659402-72659421 Msa0090580:intron 20.0%
!! TGACTAAATTAATAGAATTC+CGG - chr1_2:72659424-72659443 None:intergenic 20.0%
!!! TATGCATATTTTTATCGTTT+AGG + chr1_2:72659473-72659492 Msa0090580:intron 20.0%
!!! TTTGACTTGTTAGTTTATAA+TGG + chr1_2:72659654-72659673 Msa0090580:intron 20.0%
!! TTTATAATGGTTGAAATTTG+TGG + chr1_2:72659667-72659686 Msa0090580:intron 20.0%
!! AATTGAAACCAACTTATTAT+AGG + chr1_2:72659746-72659765 Msa0090580:intron 20.0%
!!! AAATTTAATTTAGTGCTAAC+GGG - chr1_2:72660139-72660158 None:intergenic 20.0%
!!! TATATCATATCTTTTTCACA+TGG + chr1_2:72658691-72658710 Msa0090580:intron 20.0%
!!! ATATCATATCTTTTTCACAT+GGG + chr1_2:72658692-72658711 Msa0090580:intron 20.0%
!! AAAAGACTGTAAATGTTAAA+TGG - chr1_2:72658764-72658783 None:intergenic 20.0%
!! CAAACTTAATCAATCATAAT+TGG - chr1_2:72658922-72658941 None:intergenic 20.0%
!!! TTTCTCTTTGTATTTGTTAT+AGG + chr1_2:72659143-72659162 Msa0090580:intron 20.0%
!!! AATTACTTTTTTATTCAACC+GGG - chr1_2:72659270-72659289 None:intergenic 20.0%
!! GGTTTATTCAACTAAAAAAA+TGG - chr1_2:72659332-72659351 None:intergenic 20.0%
!! TAGTTCAATGACTAAATTTA+AGG - chr1_2:72659353-72659372 None:intergenic 20.0%
!! TTATTATACTTATACAACTC+AGG - chr1_2:72659396-72659415 None:intergenic 20.0%
!! TATAAGTATAATAAACAGTC+CGG + chr1_2:72659402-72659421 Msa0090580:intron 20.0%
!! TGACTAAATTAATAGAATTC+CGG - chr1_2:72659424-72659443 None:intergenic 20.0%
!!! TATGCATATTTTTATCGTTT+AGG + chr1_2:72659473-72659492 Msa0090580:intron 20.0%
!!! TTTGACTTGTTAGTTTATAA+TGG + chr1_2:72659654-72659673 Msa0090580:intron 20.0%
!! TTTATAATGGTTGAAATTTG+TGG + chr1_2:72659667-72659686 Msa0090580:intron 20.0%
!! AATTGAAACCAACTTATTAT+AGG + chr1_2:72659746-72659765 Msa0090580:intron 20.0%
!!! AAATTTAATTTAGTGCTAAC+GGG - chr1_2:72660139-72660158 None:intergenic 20.0%
!!! TTGATGATTTTGTTGTTTTC+AGG + chr1_2:72658939-72658958 Msa0090580:intron 25.0%
!! AATTTGACTTCTACTTTGAA+GGG - chr1_2:72659717-72659736 None:intergenic 25.0%
!! AAATTTGACTTCTACTTTGA+AGG - chr1_2:72659718-72659737 None:intergenic 25.0%
!!! ATAAAACTCTAGTCTTTTTG+AGG + chr1_2:72659961-72659980 Msa0090580:three_prime_UTR 25.0%
!!! TAAAACTCTAGTCTTTTTGA+GGG + chr1_2:72659962-72659981 Msa0090580:three_prime_UTR 25.0%
!!! GAGAAGATTTTGAGTAAATT+TGG + chr1_2:72660089-72660108 Msa0090580:three_prime_UTR 25.0%
!!! TGAGTAAATTTGGTTTGATA+TGG + chr1_2:72660099-72660118 Msa0090580:three_prime_UTR 25.0%
!!! TAAATTTGGTTTGATATGGT+TGG + chr1_2:72660103-72660122 Msa0090580:three_prime_UTR 25.0%
!!! TTGATGATTTTGTTGTTTTC+AGG + chr1_2:72658939-72658958 Msa0090580:intron 25.0%
!! AATTTGACTTCTACTTTGAA+GGG - chr1_2:72659717-72659736 None:intergenic 25.0%
!! AAATTTGACTTCTACTTTGA+AGG - chr1_2:72659718-72659737 None:intergenic 25.0%
!!! ATAAAACTCTAGTCTTTTTG+AGG + chr1_2:72659961-72659980 Msa0090580:three_prime_UTR 25.0%
!!! TAAAACTCTAGTCTTTTTGA+GGG + chr1_2:72659962-72659981 Msa0090580:three_prime_UTR 25.0%
!!! GAGAAGATTTTGAGTAAATT+TGG + chr1_2:72660089-72660108 Msa0090580:three_prime_UTR 25.0%
!!! TGAGTAAATTTGGTTTGATA+TGG + chr1_2:72660099-72660118 Msa0090580:three_prime_UTR 25.0%
!!! TAAATTTGGTTTGATATGGT+TGG + chr1_2:72660103-72660122 Msa0090580:three_prime_UTR 25.0%
AATCTCATCAAGAGTTTCTA+GGG + chr1_2:72658810-72658829 Msa0090580:intron 30.0%
TAAAGAAACAAAAGAGAGAG+TGG - chr1_2:72658876-72658895 None:intergenic 30.0%
AATACCATGAAGAATGAGTT+AGG - chr1_2:72659048-72659067 None:intergenic 30.0%
TTATATAGATACTAGAGACC+CGG + chr1_2:72659249-72659268 Msa0090580:intron 30.0%
CAATGTCACAATGATTGTTA+TGG + chr1_2:72659498-72659517 Msa0090580:intron 30.0%
! TCCTGTATAAGTCGAATTTT+TGG + chr1_2:72659594-72659613 Msa0090580:intron 30.0%
ACCAAAAATTCGACTTATAC+AGG - chr1_2:72659598-72659617 None:intergenic 30.0%
! CTTTGAAGGGAAAATAAATG+TGG - chr1_2:72659704-72659723 None:intergenic 30.0%
!! AAAGGTGTCCTATAATAAGT+TGG - chr1_2:72659757-72659776 None:intergenic 30.0%
AAACCAACAAGTCAAATCAA+AGG - chr1_2:72659775-72659794 None:intergenic 30.0%
!! AAAACTCTAGTCTTTTTGAG+GGG + chr1_2:72659963-72659982 Msa0090580:three_prime_UTR 30.0%
ACTAGTCATGCTAAATTAGA+GGG - chr1_2:72660262-72660281 None:intergenic 30.0%
AATCTCATCAAGAGTTTCTA+GGG + chr1_2:72658810-72658829 Msa0090580:intron 30.0%
TAAAGAAACAAAAGAGAGAG+TGG - chr1_2:72658876-72658895 None:intergenic 30.0%
AATACCATGAAGAATGAGTT+AGG - chr1_2:72659048-72659067 None:intergenic 30.0%
TTATATAGATACTAGAGACC+CGG + chr1_2:72659249-72659268 Msa0090580:intron 30.0%
CAATGTCACAATGATTGTTA+TGG + chr1_2:72659498-72659517 Msa0090580:intron 30.0%
! TCCTGTATAAGTCGAATTTT+TGG + chr1_2:72659594-72659613 Msa0090580:intron 30.0%
ACCAAAAATTCGACTTATAC+AGG - chr1_2:72659598-72659617 None:intergenic 30.0%
! CTTTGAAGGGAAAATAAATG+TGG - chr1_2:72659704-72659723 None:intergenic 30.0%
!! AAAGGTGTCCTATAATAAGT+TGG - chr1_2:72659757-72659776 None:intergenic 30.0%
AAACCAACAAGTCAAATCAA+AGG - chr1_2:72659775-72659794 None:intergenic 30.0%
!! AAAACTCTAGTCTTTTTGAG+GGG + chr1_2:72659963-72659982 Msa0090580:three_prime_UTR 30.0%
ACTAGTCATGCTAAATTAGA+GGG - chr1_2:72660262-72660281 None:intergenic 30.0%
!! CAAATGTGGAGGTACATTTT+AGG + chr1_2:72658656-72658675 Msa0090580:intron 35.0%
! TTTTTCACATGGGCTCTTAA+TGG + chr1_2:72658702-72658721 Msa0090580:intron 35.0%
AACTCTTGATGAGATTGGAA+GGG - chr1_2:72658806-72658825 None:intergenic 35.0%
AAACTCTTGATGAGATTGGA+AGG - chr1_2:72658807-72658826 None:intergenic 35.0%
CTAGAAACTCTTGATGAGAT+TGG - chr1_2:72658811-72658830 None:intergenic 35.0%
CAATCTCATCAAGAGTTTCT+AGG + chr1_2:72658809-72658828 Msa0090580:intron 35.0%
CAATCATAATTGGAGCGATT+CGG - chr1_2:72658912-72658931 None:intergenic 35.0%
ATCTCCTAACTCATTCTTCA+TGG + chr1_2:72659041-72659060 Msa0090580:CDS 35.0%
TTATAGGATGTCAAATGCCA+AGG + chr1_2:72659159-72659178 Msa0090580:intron 35.0%
!! ACACCTTTGATTTGACTTGT+TGG + chr1_2:72659769-72659788 Msa0090580:intron 35.0%
ACACATTAACACAAGACAAC+TGG - chr1_2:72660050-72660069 None:intergenic 35.0%
CACTAGTCATGCTAAATTAG+AGG - chr1_2:72660263-72660282 None:intergenic 35.0%
ACTAGTGTACTTGTTTGGTA+TGG + chr1_2:72660276-72660295 Msa0090580:three_prime_UTR 35.0%
!! CAAATGTGGAGGTACATTTT+AGG + chr1_2:72658656-72658675 Msa0090580:intron 35.0%
! TTTTTCACATGGGCTCTTAA+TGG + chr1_2:72658702-72658721 Msa0090580:intron 35.0%
AACTCTTGATGAGATTGGAA+GGG - chr1_2:72658806-72658825 None:intergenic 35.0%
AAACTCTTGATGAGATTGGA+AGG - chr1_2:72658807-72658826 None:intergenic 35.0%
CTAGAAACTCTTGATGAGAT+TGG - chr1_2:72658811-72658830 None:intergenic 35.0%
CAATCTCATCAAGAGTTTCT+AGG + chr1_2:72658809-72658828 Msa0090580:intron 35.0%
CAATCATAATTGGAGCGATT+CGG - chr1_2:72658912-72658931 None:intergenic 35.0%
ATCTCCTAACTCATTCTTCA+TGG + chr1_2:72659041-72659060 Msa0090580:CDS 35.0%
TTATAGGATGTCAAATGCCA+AGG + chr1_2:72659159-72659178 Msa0090580:intron 35.0%
!! ACACCTTTGATTTGACTTGT+TGG + chr1_2:72659769-72659788 Msa0090580:intron 35.0%
ACACATTAACACAAGACAAC+TGG - chr1_2:72660050-72660069 None:intergenic 35.0%
CACTAGTCATGCTAAATTAG+AGG - chr1_2:72660263-72660282 None:intergenic 35.0%
ACTAGTGTACTTGTTTGGTA+TGG + chr1_2:72660276-72660295 Msa0090580:three_prime_UTR 35.0%
ACTCTTGATGAGATTGGAAG+GGG - chr1_2:72658805-72658824 None:intergenic 40.0%
AGAGAGTGGTTAACCATTGT+CGG - chr1_2:72658862-72658881 None:intergenic 40.0%
!! TCAAGAGATCGATGTCGTTT+TGG - chr1_2:72658969-72658988 None:intergenic 40.0%
! CTTACATGTTGAAGCATCCT+TGG - chr1_2:72659179-72659198 None:intergenic 40.0%
! GGAAGAAGGGTGATTAGTTA+TGG + chr1_2:72659931-72659950 Msa0090580:exon 40.0%
GCATGACTAGTGTACTTGTT+TGG + chr1_2:72660271-72660290 Msa0090580:three_prime_UTR 40.0%
ACTCTTGATGAGATTGGAAG+GGG - chr1_2:72658805-72658824 None:intergenic 40.0%
AGAGAGTGGTTAACCATTGT+CGG - chr1_2:72658862-72658881 None:intergenic 40.0%
!! TCAAGAGATCGATGTCGTTT+TGG - chr1_2:72658969-72658988 None:intergenic 40.0%
! CTTACATGTTGAAGCATCCT+TGG - chr1_2:72659179-72659198 None:intergenic 40.0%
! GGAAGAAGGGTGATTAGTTA+TGG + chr1_2:72659931-72659950 Msa0090580:exon 40.0%
GCATGACTAGTGTACTTGTT+TGG + chr1_2:72660271-72660290 Msa0090580:three_prime_UTR 40.0%
! TCATAATTGGAGCGATTCGG+AGG - chr1_2:72658909-72658928 None:intergenic 45.0%
AAACCACGACTGTTTGAGAG+TGG - chr1_2:72659839-72659858 None:intergenic 45.0%
TCTCAAACAGTCGTGGTTTG+CGG + chr1_2:72659840-72659859 Msa0090580:CDS 45.0%
CTAATCACCCTTCTTCCTGA+AGG - chr1_2:72659928-72659947 None:intergenic 45.0%
GGTATGGCTACATGTGTCAT+AGG + chr1_2:72660292-72660311 Msa0090580:three_prime_UTR 45.0%
! TCATAATTGGAGCGATTCGG+AGG - chr1_2:72658909-72658928 None:intergenic 45.0%
AAACCACGACTGTTTGAGAG+TGG - chr1_2:72659839-72659858 None:intergenic 45.0%
TCTCAAACAGTCGTGGTTTG+CGG + chr1_2:72659840-72659859 Msa0090580:CDS 45.0%
CTAATCACCCTTCTTCCTGA+AGG - chr1_2:72659928-72659947 None:intergenic 45.0%
GGTATGGCTACATGTGTCAT+AGG + chr1_2:72660292-72660311 Msa0090580:three_prime_UTR 45.0%
TCCACAGCCCTCTCAACTTT+GGG - chr1_2:72658634-72658653 None:intergenic 50.0%
TTCCACAGCCCTCTCAACTT+TGG - chr1_2:72658635-72658654 None:intergenic 50.0%
GAGGGCTGTGGAATCAAATG+TGG + chr1_2:72658642-72658661 Msa0090580:CDS 50.0%
GGCTGTGGAATCAAATGTGG+AGG + chr1_2:72658645-72658664 Msa0090580:CDS 50.0%
AGGCGACACTCACTCACATT+GGG - chr1_2:72658728-72658747 None:intergenic 50.0%
AAGGCGACACTCACTCACAT+TGG - chr1_2:72658729-72658748 None:intergenic 50.0%
CATCTCTCAACCTCCGACAA+TGG + chr1_2:72658846-72658865 Msa0090580:intron 50.0%
GAGTGGTTAACCATTGTCGG+AGG - chr1_2:72658859-72658878 None:intergenic 50.0%
! TTTTCTCAAGCTCCGCTGGT+GGG - chr1_2:72658993-72659012 None:intergenic 50.0%
! TTCCTTTTCTCAAGCTCCGC+TGG - chr1_2:72658997-72659016 None:intergenic 50.0%
TAGCCACTCTCAAACAGTCG+TGG + chr1_2:72659833-72659852 Msa0090580:CDS 50.0%
GGCAAAGCAAGGCTAACAGA+GGG + chr1_2:72659897-72659916 Msa0090580:CDS 50.0%
GCAAAGCAAGGCTAACAGAG+GGG + chr1_2:72659898-72659917 Msa0090580:CDS 50.0%
!! CTCTAGTCTTTTTGAGGGGC+TGG + chr1_2:72659967-72659986 Msa0090580:three_prime_UTR 50.0%
TCCACAGCCCTCTCAACTTT+GGG - chr1_2:72658634-72658653 None:intergenic 50.0%
TTCCACAGCCCTCTCAACTT+TGG - chr1_2:72658635-72658654 None:intergenic 50.0%
GAGGGCTGTGGAATCAAATG+TGG + chr1_2:72658642-72658661 Msa0090580:CDS 50.0%
GGCTGTGGAATCAAATGTGG+AGG + chr1_2:72658645-72658664 Msa0090580:CDS 50.0%
AGGCGACACTCACTCACATT+GGG - chr1_2:72658728-72658747 None:intergenic 50.0%
AAGGCGACACTCACTCACAT+TGG - chr1_2:72658729-72658748 None:intergenic 50.0%
CATCTCTCAACCTCCGACAA+TGG + chr1_2:72658846-72658865 Msa0090580:intron 50.0%
GAGTGGTTAACCATTGTCGG+AGG - chr1_2:72658859-72658878 None:intergenic 50.0%
! TTTTCTCAAGCTCCGCTGGT+GGG - chr1_2:72658993-72659012 None:intergenic 50.0%
! TTCCTTTTCTCAAGCTCCGC+TGG - chr1_2:72658997-72659016 None:intergenic 50.0%
TAGCCACTCTCAAACAGTCG+TGG + chr1_2:72659833-72659852 Msa0090580:CDS 50.0%
GGCAAAGCAAGGCTAACAGA+GGG + chr1_2:72659897-72659916 Msa0090580:CDS 50.0%
GCAAAGCAAGGCTAACAGAG+GGG + chr1_2:72659898-72659917 Msa0090580:CDS 50.0%
!! CTCTAGTCTTTTTGAGGGGC+TGG + chr1_2:72659967-72659986 Msa0090580:three_prime_UTR 50.0%
ACCCAAAGTTGAGAGGGCTG+TGG + chr1_2:72658630-72658649 Msa0090580:CDS 55.0%
CGATCTCTTGAACCCACCAG+CGG + chr1_2:72658978-72658997 Msa0090580:CDS 55.0%
! CTTTTCTCAAGCTCCGCTGG+TGG - chr1_2:72658994-72659013 None:intergenic 55.0%
CACCAGCGGAGCTTGAGAAA+AGG + chr1_2:72658992-72659011 Msa0090580:CDS 55.0%
GGTTTGCGGCAACTGTCAGA+CGG + chr1_2:72659854-72659873 Msa0090580:CDS 55.0%
ACGGTATTGTGCCAGCCAAC+CGG + chr1_2:72659873-72659892 Msa0090580:CDS 55.0%
!! TGTTAGCCTTGCTTTGCCGC+CGG - chr1_2:72659895-72659914 None:intergenic 55.0%
CGGCAAAGCAAGGCTAACAG+AGG + chr1_2:72659896-72659915 Msa0090580:CDS 55.0%
!! TAACAGAGGGGTGCTCCTTC+AGG + chr1_2:72659910-72659929 Msa0090580:CDS 55.0%
GGGTGCTCCTTCAGGAAGAA+GGG + chr1_2:72659918-72659937 Msa0090580:CDS 55.0%
ACCCAAAGTTGAGAGGGCTG+TGG + chr1_2:72658630-72658649 Msa0090580:CDS 55.0%
CGATCTCTTGAACCCACCAG+CGG + chr1_2:72658978-72658997 Msa0090580:CDS 55.0%
! CTTTTCTCAAGCTCCGCTGG+TGG - chr1_2:72658994-72659013 None:intergenic 55.0%
CACCAGCGGAGCTTGAGAAA+AGG + chr1_2:72658992-72659011 Msa0090580:CDS 55.0%
GGTTTGCGGCAACTGTCAGA+CGG + chr1_2:72659854-72659873 Msa0090580:CDS 55.0%
ACGGTATTGTGCCAGCCAAC+CGG + chr1_2:72659873-72659892 Msa0090580:CDS 55.0%
!! TGTTAGCCTTGCTTTGCCGC+CGG - chr1_2:72659895-72659914 None:intergenic 55.0%
CGGCAAAGCAAGGCTAACAG+AGG + chr1_2:72659896-72659915 Msa0090580:CDS 55.0%
!! TAACAGAGGGGTGCTCCTTC+AGG + chr1_2:72659910-72659929 Msa0090580:CDS 55.0%
GGGTGCTCCTTCAGGAAGAA+GGG + chr1_2:72659918-72659937 Msa0090580:CDS 55.0%
GTATTGTGCCAGCCAACCGG+CGG + chr1_2:72659876-72659895 Msa0090580:CDS 60.0%
! GGGGTGCTCCTTCAGGAAGA+AGG + chr1_2:72659917-72659936 Msa0090580:CDS 60.0%
GTATTGTGCCAGCCAACCGG+CGG + chr1_2:72659876-72659895 Msa0090580:CDS 60.0%
! GGGGTGCTCCTTCAGGAAGA+AGG + chr1_2:72659917-72659936 Msa0090580:CDS 60.0%
AAGGGGCTGCGACTGCGAGA+AGG - chr1_2:72658788-72658807 None:intergenic 65.0%
! TTGCTTTGCCGCCGGTTGGC+TGG - chr1_2:72659887-72659906 None:intergenic 65.0%
AGCCAACCGGCGGCAAAGCA+AGG + chr1_2:72659886-72659905 Msa0090580:CDS 65.0%
AGCCTTGCTTTGCCGCCGGT+TGG - chr1_2:72659891-72659910 None:intergenic 65.0%
AAGGGGCTGCGACTGCGAGA+AGG - chr1_2:72658788-72658807 None:intergenic 65.0%
! TTGCTTTGCCGCCGGTTGGC+TGG - chr1_2:72659887-72659906 None:intergenic 65.0%
AGCCAACCGGCGGCAAAGCA+AGG + chr1_2:72659886-72659905 Msa0090580:CDS 65.0%
AGCCTTGCTTTGCCGCCGGT+TGG - chr1_2:72659891-72659910 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr1_2 gene 72658625 72660326 72658625 ID=Msa0090580;Name=Msa0090580
chr1_2 mRNA 72658625 72660326 72658625 ID=Msa0090580-mRNA-1;Parent=Msa0090580;Name=Msa0090580-mRNA-1;_AED=0.20;_eAED=0.20;_QI=0|0.75|0.8|0.8|0.75|0.6|5|342|99
chr1_2 exon 72658625 72658666 72658625 ID=Msa0090580-mRNA-1:exon:25226;Parent=Msa0090580-mRNA-1
chr1_2 exon 72658961 72659062 72658961 ID=Msa0090580-mRNA-1:exon:25227;Parent=Msa0090580-mRNA-1
chr1_2 exon 72659165 72659193 72659165 ID=Msa0090580-mRNA-1:exon:25228;Parent=Msa0090580-mRNA-1
chr1_2 exon 72659821 72660162 72659821 ID=Msa0090580-mRNA-1:exon:25229;Parent=Msa0090580-mRNA-1
chr1_2 exon 72660200 72660326 72660200 ID=Msa0090580-mRNA-1:exon:25230;Parent=Msa0090580-mRNA-1
chr1_2 CDS 72658625 72658666 72658625 ID=Msa0090580-mRNA-1:cds;Parent=Msa0090580-mRNA-1
chr1_2 CDS 72658961 72659062 72658961 ID=Msa0090580-mRNA-1:cds;Parent=Msa0090580-mRNA-1
chr1_2 CDS 72659165 72659193 72659165 ID=Msa0090580-mRNA-1:cds;Parent=Msa0090580-mRNA-1
chr1_2 CDS 72659821 72659947 72659821 ID=Msa0090580-mRNA-1:cds;Parent=Msa0090580-mRNA-1
chr1_2 three_prime_UTR 72659948 72660162 72659948 ID=Msa0090580-mRNA-1:three_prime_utr;Parent=Msa0090580-mRNA-1
chr1_2 three_prime_UTR 72660200 72660326 72660200 ID=Msa0090580-mRNA-1:three_prime_utr;Parent=Msa0090580-mRNA-1
Gene Sequence

>Msa0090580

ATGGGACCCAAAGTTGAGAGGGCTGTGGAATCAAATGTGGAGGTTCTCCAAAACGACATCGATCTCTTGAACCCACCAGCGGAGCTTGAGAAAAGGAAGCACAAGCTCAAGCGTCTCGTTCAATCTCCTAACTCATTCTTCATGGATGTCAAATGCCAAGGATGCTTCAACATAACTACTGTATTTAGCCACTCTCAAACAGTCGTGGTTTGCGGCAACTGTCAGACGGTATTGTGCCAGCCAACCGGCGGCAAAGCAAGGCTAACAGAGGGGTGCTCCTTCAGGAAGAAGGGTGATTAG
Protein sequence

>Msa0090580

MGPKVERAVESNVEVLQNDIDLLNPPAELEKRKHKLKRLVQSPNSFFMDVKCQGCFNITTVFSHSQTVVVCGNCQTVLCQPTGGKARLTEGCSFRKKGD*