AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0725440

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0725440 MtrunA17_Chr5g0417101 100.000 120 0 0 1 120 1 120 6.22e-83 238
Msa0725440 MtrunA17_Chr7g0217171 96.667 120 4 0 1 120 1 120 2.26e-80 231
Msa0725440 MtrunA17_Chr8g0361721 96.667 120 4 0 1 120 1 120 1.92e-79 229
Msa0725440 MtrunA17_Chr7g0217651 75.510 49 12 0 1 49 1 49 1.98e-21 80.5
Msa0725440 MtrunA17_Chr8g0343321 72.727 55 14 1 66 119 66 120 4.56e-18 73.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0725440 AT1G26880.1 93.333 120 8 0 1 120 1 120 2.14e-77 224
Msa0725440 AT1G69620.1 93.277 119 8 0 1 119 1 119 1.67e-76 222
Msa0725440 AT3G28900.1 88.333 120 14 0 1 120 1 120 3.00e-73 214
Msa0725440 AT1G26880.2 91.304 92 8 0 1 92 1 92 8.09e-58 174

Find 58 sgRNAs with CRISPR-Local

Find 149 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTATGTTAATTGTGTCTTAT+TGG 0.127608 5_1:-25149528 Msa0725440:three_prime_UTR
GCCTCACCGCTTAGCTTATT+AGG 0.191767 5_1:+25151967 None:intergenic
TGAATTCTCTTGCCGGTAAC+AGG 0.241048 5_1:+25151695 None:intergenic
TTGCCTCCAAGGCATAATTT+TGG 0.249610 5_1:-25149638 Msa0725440:exon
TAGGATCATTAGGGCTTTCT+TGG 0.253359 5_1:-25149718 Msa0725440:CDS
CCTCACCGCTTAGCTTATTA+GGG 0.289547 5_1:+25151968 None:intergenic
CCTGGTTCTATAGGATCATT+AGG 0.306736 5_1:-25149728 Msa0725440:intron
CACAAAGGCGCTGAAATTAA+AGG 0.329355 5_1:+25149401 None:intergenic
AACCCTGTGTTGGTTGGATT+TGG 0.331681 5_1:+25151876 None:intergenic
TTCACAGTCCTACGGTTTCT+AGG 0.332169 5_1:+25149881 None:intergenic
AGCAGAAGATTGATCGAAAA+TGG 0.350396 5_1:-25151938 Msa0725440:exon
AGAGCAAAAGATTGTGAAAA+AGG 0.358157 5_1:-25149691 Msa0725440:CDS
TTTGTCTGGAGGTGCTGTTC+GGG 0.362626 5_1:-25149840 Msa0725440:CDS
CTTTAATTTCAGCGCCTTTG+TGG 0.375714 5_1:-25149400 Msa0725440:three_prime_UTR
AGGACTGTGAACCGTGCTTA+TGG 0.389130 5_1:-25149869 Msa0725440:CDS
TACTCCTTGAATTCTCTTGC+CGG 0.391773 5_1:+25151688 None:intergenic
TATTGACAACCGTTAGATCT+AGG 0.393756 5_1:-25152043 None:intergenic
GGACCTAAGTGCCCTGTTAC+CGG 0.400005 5_1:-25151707 Msa0725440:CDS
GCTGAAATTAAAGGTACTAT+TGG 0.450752 5_1:+25149410 None:intergenic
CTGGAGGTGCTGTTCGGGAA+AGG 0.453586 5_1:-25149835 Msa0725440:intron
TAAGCTAAGCGGTGAGGCAG+AGG 0.476454 5_1:-25151962 Msa0725440:five_prime_UTR
AAGATTGTCATCTCATTTCG+TGG 0.479538 5_1:+25149614 None:intergenic
AATTCAGAAGGCAAAGGAAA+AGG 0.486375 5_1:-25149661 Msa0725440:CDS
CGTGGTCCAAAATTATGCCT+TGG 0.493061 5_1:+25149632 None:intergenic
CTGGTTCTATAGGATCATTA+GGG 0.506788 5_1:-25149727 Msa0725440:intron
GTTACCGGCAAGAGAATTCA+AGG 0.510157 5_1:-25151692 Msa0725440:CDS
AAAGGAAAAGGTTGCCTCCA+AGG 0.514108 5_1:-25149649 Msa0725440:CDS
TTTGACAACCCTGTGTTGGT+TGG 0.514440 5_1:+25151870 None:intergenic
CTACAGAGTACAAGAGATCA+AGG 0.519507 5_1:-25149907 Msa0725440:CDS
GGTCCAAAATTATGCCTTGG+AGG 0.546910 5_1:+25149635 None:intergenic
CACAGGGTTGTCAAAACCCC+TGG 0.556629 5_1:-25151862 Msa0725440:intron
ATTCTCATTTATACTCCTTG+CGG 0.556881 5_1:-25152015 Msa0725440:five_prime_UTR
TTGCCGGTAACAGGGCACTT+AGG 0.561871 5_1:+25151704 None:intergenic
CTTGATCTCTTGTACTCTGT+AGG 0.565264 5_1:+25149908 None:intergenic
CAACCAAATCCAACCAACAC+AGG 0.569419 5_1:-25151879 Msa0725440:CDS
AATTAAAGGTACTATTGGTA+AGG 0.569696 5_1:+25149415 None:intergenic
ACTACTAAGAAGAGAGCTAG+TGG 0.569876 5_1:-25151728 Msa0725440:CDS
CAATAAGACACAATTAACAT+AGG 0.581454 5_1:+25149529 None:intergenic
GAATTCTCTTGCCGGTAACA+GGG 0.583734 5_1:+25151696 None:intergenic
AAAGGTCCTCAAAATTCAGA+AGG 0.596465 5_1:-25149673 Msa0725440:CDS
AATTGCAGATTCCACACTTG+AGG 0.607246 5_1:-25149931 Msa0725440:intron
AAACTCAATAATAAGCCGCA+AGG 0.616032 5_1:+25152000 None:intergenic
GTAGCTGTGACGACGGCGAT+AGG 0.621925 5_1:+25151903 None:intergenic
GAATGATAACCTAGATCTAA+CGG 0.622144 5_1:+25152034 None:intergenic
TACTCTGTAGGCCTCAAGTG+TGG 0.623172 5_1:+25149920 None:intergenic
CCCTAATAAGCTAAGCGGTG+AGG 0.628679 5_1:-25151968 Msa0725440:five_prime_UTR
CCTCAAAATTCAGAAGGCAA+AGG 0.629318 5_1:-25149667 Msa0725440:CDS
AACCAAATCCAACCAACACA+GGG 0.630763 5_1:-25151878 Msa0725440:CDS
AATTCAAAACTGTATCACAA+TGG 0.638663 5_1:+25149470 None:intergenic
TGGTTGCGTAGCTGTGACGA+CGG 0.639865 5_1:+25151896 None:intergenic
AAGCACGGTTCACAGTCCTA+CGG 0.644276 5_1:+25149873 None:intergenic
ACAAAACTCCGCCATAAGCA+CGG 0.644997 5_1:+25149858 None:intergenic
ACTGTGAACCGTGCTTATGG+CGG 0.659783 5_1:-25149866 Msa0725440:CDS
CAAGGTTGCCTAGAAACCGT+AGG 0.669264 5_1:-25149889 Msa0725440:CDS
GAAAACCCTAATAAGCTAAG+CGG 0.690771 5_1:-25151973 Msa0725440:five_prime_UTR
GACTTAGAAAATAACCACAA+AGG 0.691297 5_1:+25149386 None:intergenic
TGGTAAGGTACAGTAACACA+AGG 0.692362 5_1:+25149430 None:intergenic
ACAGATAAACTTCAACTGTG+CGG 0.721668 5_1:+25149360 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GATTTGTTATATTTTGAAAT+TGG - chr5_1:25149517-25149536 Msa0725440:three_prime_UTR 15.0%
!!! ATACAGGAATAATTTTATAA+AGG + chr5_1:25150527-25150546 None:intergenic 15.0%
!! TTAAATTTCATAAACTAAAC+TGG + chr5_1:25150586-25150605 None:intergenic 15.0%
!! AAAAATTATACACAAATCTT+TGG - chr5_1:25150858-25150877 Msa0725440:intron 15.0%
!!! CATTTAATGATTTGATTTAA+TGG - chr5_1:25151539-25151558 Msa0725440:intron 15.0%
!! AAATTGAAATTTACTTACCA+GGG + chr5_1:25149473-25149492 None:intergenic 20.0%
!!! AATATAACAAATCTGAGATT+CGG + chr5_1:25149510-25149529 None:intergenic 20.0%
!! GGAAAAAAAAAAATCAAAAG+AGG + chr5_1:25149664-25149683 None:intergenic 20.0%
!!! TTGATTTTTTTTTTTCCGTT+TGG - chr5_1:25149667-25149686 Msa0725440:CDS 20.0%
!! AAGTTGTGAGTATAAATTAA+TGG - chr5_1:25150710-25150729 Msa0725440:intron 20.0%
!! AGTTGTGAGTATAAATTAAT+GGG - chr5_1:25150711-25150730 Msa0725440:intron 20.0%
!! AAACATGAAGCAATAAAAAA+TGG + chr5_1:25150742-25150761 None:intergenic 20.0%
!! TAGAATTTCAAAAACTAAAC+TGG + chr5_1:25150987-25151006 None:intergenic 20.0%
!!! TATTATATGTCTCTTACTTT+TGG - chr5_1:25151317-25151336 Msa0725440:intron 20.0%
!!! TAATGATTTGATTTAATGGA+TGG - chr5_1:25151543-25151562 Msa0725440:intron 20.0%
!!! TTTTGAATTTTCTGGTTTAT+TGG - chr5_1:25151859-25151878 Msa0725440:intron 20.0%
! AATTGAAATTTACTTACCAG+GGG + chr5_1:25149472-25149491 None:intergenic 25.0%
! GAAATTGAAATTTACTTACC+AGG + chr5_1:25149474-25149493 None:intergenic 25.0%
!! ATTTTGTGATGTTGAAATGT+AGG - chr5_1:25149545-25149564 Msa0725440:three_prime_UTR 25.0%
!!! TTTTTTTTTCCGTTTGGAAA+TGG - chr5_1:25149673-25149692 Msa0725440:CDS 25.0%
!!! TTTTTTTTCCGTTTGGAAAT+GGG - chr5_1:25149674-25149693 Msa0725440:CDS 25.0%
!!! AGCTGTTTTTTAAGCTATAT+TGG - chr5_1:25149912-25149931 Msa0725440:CDS 25.0%
! CACAAACATCAAATTACTAA+TGG + chr5_1:25150049-25150068 None:intergenic 25.0%
! GTTAGATTAATGTGTATAAC+TGG - chr5_1:25150125-25150144 Msa0725440:intron 25.0%
!!! AATGGGAAATTAGTTTGTTT+AGG - chr5_1:25150166-25150185 Msa0725440:intron 25.0%
! TAACATGAAGCTATAAACAA+AGG + chr5_1:25150217-25150236 None:intergenic 25.0%
!! ACCTTTTAAACTTGTAAATG+TGG - chr5_1:25150375-25150394 Msa0725440:intron 25.0%
! ACCACATTTACAAGTTTAAA+AGG + chr5_1:25150379-25150398 None:intergenic 25.0%
! TAAACTTGTAAATGTGGTTA+TGG - chr5_1:25150381-25150400 Msa0725440:intron 25.0%
!!! CAAACTTTTAAACATTGACT+TGG - chr5_1:25150445-25150464 Msa0725440:intron 25.0%
! TGTGTGAAATTAAAGAAACA+TGG - chr5_1:25150669-25150688 Msa0725440:intron 25.0%
! ATGTTTATTGCTTCATGTAT+GGG - chr5_1:25150756-25150775 Msa0725440:intron 25.0%
!! TAACAAGTATTTTGTGCTTA+TGG - chr5_1:25150787-25150806 Msa0725440:intron 25.0%
! ATGCAAAACTTATAAAGATC+AGG - chr5_1:25150887-25150906 Msa0725440:intron 25.0%
!! GTTAATTTTGTTACGAGTTA+AGG + chr5_1:25151036-25151055 None:intergenic 25.0%
! GTAACAAAATTAACAGCAAA+AGG - chr5_1:25151042-25151061 Msa0725440:intron 25.0%
!! TTGCTTTGTTATAGAATGTT+TGG - chr5_1:25151146-25151165 Msa0725440:intron 25.0%
! AGTTAAAAAACATATCACAG+AGG + chr5_1:25151289-25151308 None:intergenic 25.0%
! GTAAGAGACATATAATACAA+TGG + chr5_1:25151314-25151333 None:intergenic 25.0%
!! AAATGAGATGACAATCTTTT+TGG - chr5_1:25151706-25151725 Msa0725440:CDS 25.0%
! CAATAAGACACAATTAACAT+AGG + chr5_1:25151789-25151808 None:intergenic 25.0%
! CTATGTTAATTGTGTCTTAT+TGG - chr5_1:25151787-25151806 Msa0725440:intron 25.0%
! AATTCAAAACTGTATCACAA+TGG + chr5_1:25151848-25151867 None:intergenic 25.0%
!!! TGATACAGTTTTGAATTTTC+TGG - chr5_1:25151851-25151870 Msa0725440:intron 25.0%
!!! AATTAAAGGTACTATTGGTA+AGG + chr5_1:25151903-25151922 None:intergenic 25.0%
GAATGATAACCTAGATCTAA+CGG + chr5_1:25149284-25149303 None:intergenic 30.0%
ATTCTCATTTATACTCCTTG+CGG - chr5_1:25149300-25149319 Msa0725440:three_prime_UTR 30.0%
ACAAAAAACCCATTTCCAAA+CGG + chr5_1:25149685-25149704 None:intergenic 30.0%
TCAAATAAGCCAAATCCAAA+CGG - chr5_1:25150022-25150041 Msa0725440:intron 30.0%
AGCTGTGTGAAATAAAGAAA+TGG - chr5_1:25150148-25150167 Msa0725440:intron 30.0%
GCTGTGTGAAATAAAGAAAT+GGG - chr5_1:25150149-25150168 Msa0725440:intron 30.0%
!! TTAGTTTGTTTAGGAGAAGT+TGG - chr5_1:25150175-25150194 Msa0725440:intron 30.0%
!! TAGTTTGTTTAGGAGAAGTT+GGG - chr5_1:25150176-25150195 Msa0725440:intron 30.0%
TAGCTTCATGTTATGATTTG+TGG - chr5_1:25150224-25150243 Msa0725440:intron 30.0%
GGAAAAACATATCAAACTCA+AGG + chr5_1:25150332-25150351 None:intergenic 30.0%
! TAAATGTGGTTATGGCTTTT+TGG - chr5_1:25150389-25150408 Msa0725440:intron 30.0%
! TTAACAACTAGTTTGTGCTT+AGG - chr5_1:25150412-25150431 Msa0725440:intron 30.0%
!! GAGAACTCTTTAATTTGTAC+AGG + chr5_1:25150481-25150500 None:intergenic 30.0%
TCCTGTATAATATGAACACT+TGG - chr5_1:25150539-25150558 Msa0725440:intron 30.0%
TCCAAGTGTTCATATTATAC+AGG + chr5_1:25150543-25150562 None:intergenic 30.0%
TGTTAGATTATTGTGTTACG+TGG - chr5_1:25150644-25150663 Msa0725440:intron 30.0%
GTTAGATTATTGTGTTACGT+GGG - chr5_1:25150645-25150664 Msa0725440:intron 30.0%
CATGTTTATTGCTTCATGTA+TGG - chr5_1:25150755-25150774 Msa0725440:intron 30.0%
!!! GGTTTGTATGCTTATTTTAC+AGG - chr5_1:25151501-25151520 Msa0725440:intron 30.0%
AATCTGATTATATTTGTCCC+TGG - chr5_1:25151569-25151588 Msa0725440:intron 30.0%
AGAGCAAAAGATTGTGAAAA+AGG - chr5_1:25151624-25151643 Msa0725440:intron 30.0%
!! AATCTTTTTGGAAGACTCTT+AGG - chr5_1:25151718-25151737 Msa0725440:CDS 30.0%
!! GCTGAAATTAAAGGTACTAT+TGG + chr5_1:25151908-25151927 None:intergenic 30.0%
GACTTAGAAAATAACCACAA+AGG + chr5_1:25151932-25151951 None:intergenic 30.0%
AAACTCAATAATAAGCCGCA+AGG + chr5_1:25149318-25149337 None:intergenic 35.0%
GAAAACCCTAATAAGCTAAG+CGG - chr5_1:25149342-25149361 Msa0725440:three_prime_UTR 35.0%
AGCAGAAGATTGATCGAAAA+TGG - chr5_1:25149377-25149396 Msa0725440:three_prime_UTR 35.0%
TTGTGATGTTGAAATGTAGG+TGG - chr5_1:25149548-25149567 Msa0725440:three_prime_UTR 35.0%
ACATAAGCTGGTTATCCAAA+CGG + chr5_1:25149790-25149809 None:intergenic 35.0%
!! TAAGTTTTGTGTGACTGTCT+GGG + chr5_1:25149987-25150006 None:intergenic 35.0%
!! ATAAGTTTTGTGTGACTGTC+TGG + chr5_1:25149988-25150007 None:intergenic 35.0%
CAAATAAGCCAAATCCAAAC+GGG - chr5_1:25150023-25150042 Msa0725440:intron 35.0%
CAAAGCATATCAAACACAAG+GGG + chr5_1:25150353-25150372 None:intergenic 35.0%
GCAAAGCATATCAAACACAA+GGG + chr5_1:25150354-25150373 None:intergenic 35.0%
AGCAAAGCATATCAAACACA+AGG + chr5_1:25150355-25150374 None:intergenic 35.0%
!! TATGTCTCTTACTTTTGGTC+TGG - chr5_1:25151322-25151341 Msa0725440:intron 35.0%
!! ATATTTGTCCCTGGTTCTAT+AGG - chr5_1:25151578-25151597 Msa0725440:intron 35.0%
CTAATGATCCTATAGAACCA+GGG + chr5_1:25151589-25151608 None:intergenic 35.0%
!! CTGGTTCTATAGGATCATTA+GGG - chr5_1:25151588-25151607 Msa0725440:intron 35.0%
AAAGGTCCTCAAAATTCAGA+AGG - chr5_1:25151642-25151661 Msa0725440:intron 35.0%
AATTCAGAAGGCAAAGGAAA+AGG - chr5_1:25151654-25151673 Msa0725440:intron 35.0%
AAGATTGTCATCTCATTTCG+TGG + chr5_1:25151704-25151723 None:intergenic 35.0%
ACAGATAAACTTCAACTGTG+CGG + chr5_1:25151958-25151977 None:intergenic 35.0%
!!! ACTGTGTTGTAGTTTTTTGC+TGG - chr5_1:25152007-25152026 Msa0725440:five_prime_UTR 35.0%
AACCAAATCCAACCAACACA+GGG - chr5_1:25149437-25149456 Msa0725440:three_prime_UTR 40.0%
ACTACTAAGAAGAGAGCTAG+TGG - chr5_1:25149587-25149606 Msa0725440:three_prime_UTR 40.0%
TACTCCTTGAATTCTCTTGC+CGG + chr5_1:25149630-25149649 None:intergenic 40.0%
ACCTTGATTTAGTACCCGTT+TGG - chr5_1:25149772-25149791 Msa0725440:intron 40.0%
TCCAAACGGGTACTAAATCA+AGG + chr5_1:25149776-25149795 None:intergenic 40.0%
CATAAGCTGGTTATCCAAAC+GGG + chr5_1:25149789-25149808 None:intergenic 40.0%
TAAGCGCTTATCACATAAGC+TGG + chr5_1:25149802-25149821 None:intergenic 40.0%
!! AAGTTTTGTGTGACTGTCTG+GGG + chr5_1:25149986-25150005 None:intergenic 40.0%
CAAATTACTAATGGCCCGTT+TGG + chr5_1:25150040-25150059 None:intergenic 40.0%
TAGGAGAAGTTGGGAATTGA+CGG - chr5_1:25150185-25150204 Msa0725440:intron 40.0%
TGGCAATGACATTCACAATC+TGG + chr5_1:25150566-25150585 None:intergenic 40.0%
TGTTACGAGTTAAGGCTTCA+AGG + chr5_1:25151028-25151047 None:intergenic 40.0%
TAGAATTCGTGACCAATCAC+AGG - chr5_1:25151195-25151214 Msa0725440:intron 40.0%
AATTGCAGATTCCACACTTG+AGG - chr5_1:25151384-25151403 Msa0725440:intron 40.0%
CTTGATCTCTTGTACTCTGT+AGG + chr5_1:25151410-25151429 None:intergenic 40.0%
CTACAGAGTACAAGAGATCA+AGG - chr5_1:25151408-25151427 Msa0725440:intron 40.0%
CCTAATGATCCTATAGAACC+AGG + chr5_1:25151590-25151609 None:intergenic 40.0%
!! CCTGGTTCTATAGGATCATT+AGG - chr5_1:25151587-25151606 Msa0725440:intron 40.0%
TAGGATCATTAGGGCTTTCT+TGG - chr5_1:25151597-25151616 Msa0725440:intron 40.0%
! CCTTTGCCTTCTGAATTTTG+AGG + chr5_1:25151651-25151670 None:intergenic 40.0%
CCTCAAAATTCAGAAGGCAA+AGG - chr5_1:25151648-25151667 Msa0725440:intron 40.0%
! TTGCCTCCAAGGCATAATTT+TGG - chr5_1:25151677-25151696 Msa0725440:intron 40.0%
TGGTAAGGTACAGTAACACA+AGG + chr5_1:25151888-25151907 None:intergenic 40.0%
!! CACAAAGGCGCTGAAATTAA+AGG + chr5_1:25151917-25151936 None:intergenic 40.0%
! CTTTAATTTCAGCGCCTTTG+TGG - chr5_1:25151915-25151934 Msa0725440:CDS 40.0%
CCTCACCGCTTAGCTTATTA+GGG + chr5_1:25149350-25149369 None:intergenic 45.0%
CAACCAAATCCAACCAACAC+AGG - chr5_1:25149436-25149455 Msa0725440:three_prime_UTR 45.0%
!! AACCCTGTGTTGGTTGGATT+TGG + chr5_1:25149442-25149461 None:intergenic 45.0%
! TTTGACAACCCTGTGTTGGT+TGG + chr5_1:25149448-25149467 None:intergenic 45.0%
GAATTCTCTTGCCGGTAACA+GGG + chr5_1:25149622-25149641 None:intergenic 45.0%
TGAATTCTCTTGCCGGTAAC+AGG + chr5_1:25149623-25149642 None:intergenic 45.0%
GTTACCGGCAAGAGAATTCA+AGG - chr5_1:25149623-25149642 Msa0725440:three_prime_UTR 45.0%
ACTAATGGCCCGTTTGGATT+TGG + chr5_1:25150034-25150053 None:intergenic 45.0%
! CAACAGAACTAGCCTGTGAT+TGG + chr5_1:25151210-25151229 None:intergenic 45.0%
!! CAATCACAGGCTAGTTCTGT+TGG - chr5_1:25151208-25151227 Msa0725440:intron 45.0%
TTCACAGTCCTACGGTTTCT+AGG + chr5_1:25151437-25151456 None:intergenic 45.0%
ACAAAACTCCGCCATAAGCA+CGG + chr5_1:25151460-25151479 None:intergenic 45.0%
!!! TTTTGTCTGGAGGTGCTGTT+CGG - chr5_1:25151474-25151493 Msa0725440:intron 45.0%
AAAGGAAAAGGTTGCCTCCA+AGG - chr5_1:25151666-25151685 Msa0725440:intron 45.0%
GGTCCAAAATTATGCCTTGG+AGG + chr5_1:25151683-25151702 None:intergenic 45.0%
CGTGGTCCAAAATTATGCCT+TGG + chr5_1:25151686-25151705 None:intergenic 45.0%
!! ATAGTAATATTAATTAAAAA+TGG + chr5_1:25151071-25151090 None:intergenic 5.0%
CCCTAATAAGCTAAGCGGTG+AGG - chr5_1:25149347-25149366 Msa0725440:three_prime_UTR 50.0%
GCCTCACCGCTTAGCTTATT+AGG + chr5_1:25149351-25149370 None:intergenic 50.0%
!! GGGTTTTGACAACCCTGTGT+TGG + chr5_1:25149452-25149471 None:intergenic 50.0%
TACTCTGTAGGCCTCAAGTG+TGG + chr5_1:25151398-25151417 None:intergenic 50.0%
CAAGGTTGCCTAGAAACCGT+AGG - chr5_1:25151426-25151445 Msa0725440:intron 50.0%
AAGCACGGTTCACAGTCCTA+CGG + chr5_1:25151445-25151464 None:intergenic 50.0%
AGGACTGTGAACCGTGCTTA+TGG - chr5_1:25151446-25151465 Msa0725440:intron 50.0%
ACTGTGAACCGTGCTTATGG+CGG - chr5_1:25151449-25151468 Msa0725440:intron 50.0%
!! GCTTATGGCGGAGTTTTGTC+TGG - chr5_1:25151461-25151480 Msa0725440:intron 50.0%
!! TATGGCGGAGTTTTGTCTGG+AGG - chr5_1:25151464-25151483 Msa0725440:intron 50.0%
!! TTTGTCTGGAGGTGCTGTTC+GGG - chr5_1:25151475-25151494 Msa0725440:intron 50.0%
TAAGCTAAGCGGTGAGGCAG+AGG - chr5_1:25149353-25149372 Msa0725440:three_prime_UTR 55.0%
TGGTTGCGTAGCTGTGACGA+CGG + chr5_1:25149422-25149441 None:intergenic 55.0%
CACAGGGTTGTCAAAACCCC+TGG - chr5_1:25149453-25149472 Msa0725440:three_prime_UTR 55.0%
GGACCTAAGTGCCCTGTTAC+CGG - chr5_1:25149608-25149627 Msa0725440:three_prime_UTR 55.0%
! TTGCCGGTAACAGGGCACTT+AGG + chr5_1:25149614-25149633 None:intergenic 55.0%
GTAGCTGTGACGACGGCGAT+AGG + chr5_1:25149415-25149434 None:intergenic 60.0%
!! CTGGAGGTGCTGTTCGGGAA+AGG - chr5_1:25151480-25151499 Msa0725440:intron 60.0%
Chromosome Type Strat End Strand Name
chr5_1 gene 25149276 25152061 25149276 ID=Msa0725440;Name=Msa0725440
chr5_1 mRNA 25149276 25152061 25149276 ID=Msa0725440-mRNA-1;Parent=Msa0725440;Name=Msa0725440-mRNA-1;_AED=0.03;_eAED=0.03;_QI=120|1|1|1|1|1|4|368|120
chr5_1 exon 25149276 25149737 25149276 ID=Msa0725440-mRNA-1:exon:9111;Parent=Msa0725440-mRNA-1
chr5_1 exon 25149836 25149945 25149836 ID=Msa0725440-mRNA-1:exon:9110;Parent=Msa0725440-mRNA-1
chr5_1 exon 25151691 25151770 25151691 ID=Msa0725440-mRNA-1:exon:9109;Parent=Msa0725440-mRNA-1
chr5_1 exon 25151863 25152061 25151863 ID=Msa0725440-mRNA-1:exon:9108;Parent=Msa0725440-mRNA-1
chr5_1 five_prime_UTR 25151942 25152061 25151942 ID=Msa0725440-mRNA-1:five_prime_utr;Parent=Msa0725440-mRNA-1
chr5_1 CDS 25151863 25151941 25151863 ID=Msa0725440-mRNA-1:cds;Parent=Msa0725440-mRNA-1
chr5_1 CDS 25151691 25151770 25151691 ID=Msa0725440-mRNA-1:cds;Parent=Msa0725440-mRNA-1
chr5_1 CDS 25149836 25149945 25149836 ID=Msa0725440-mRNA-1:cds;Parent=Msa0725440-mRNA-1
chr5_1 CDS 25149644 25149737 25149644 ID=Msa0725440-mRNA-1:cds;Parent=Msa0725440-mRNA-1
chr5_1 three_prime_UTR 25149276 25149643 25149276 ID=Msa0725440-mRNA-1:three_prime_utr;Parent=Msa0725440-mRNA-1
Gene Sequence

>Msa0725440

ATGGTGCAGCGTCTCACCTATCGCCGTCGTCACAGCTACGCAACCAAATCCAACCAACACAGGGTTGTCAAAACCCCTGGTGGTAAGCTTGTTTATCAGACTACTAAGAAGAGAGCTAGTGGACCTAAGTGCCCTGTTACCGGCAAGAGAATTCAAGGAATTCCACACTTGAGGCCTACAGAGTACAAGAGATCAAGGTTGCCTAGAAACCGTAGGACTGTGAACCGTGCTTATGGCGGAGTTTTGTCTGGAGGTGCTGTTCGGGAAAGGATCATTAGGGCTTTCTTGGTTGAAGAGCAAAAGATTGTGAAAAAGGTCCTCAAAATTCAGAAGGCAAAGGAAAAGGTTGCCTCCAAGGCATAA
Protein sequence

>Msa0725440

MVQRLTYRRRHSYATKSNQHRVVKTPGGKLVYQTTKKRASGPKCPVTGKRIQGIPHLRPTEYKRSRLPRNRRTVNRAYGGVLSGGAVRERIIRAFLVEEQKIVKKVLKIQKAKEKVASKA*