AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1418770


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1418770 MtrunA17_Chr8g0392251 100.000 140 0 0 1 140 1 140 9.25e-101 285
Msa1418770 MtrunA17_Chr8g0392191 100.000 140 0 0 1 140 1 140 9.25e-101 285
Msa1418770 MtrunA17_Chr5g0432551 99.286 140 1 0 1 140 1 140 2.08e-100 283
Msa1418770 MtrunA17_Chr4g0044571 95.000 140 7 0 1 140 4 143 2.30e-96 273
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1418770 AT2G09990.1 89.928 139 14 0 2 140 8 146 5.38e-92 263
Msa1418770 AT5G18380.1 89.209 139 15 0 2 140 8 146 9.21e-92 262
Msa1418770 AT3G04230.1 84.173 139 22 0 2 140 8 146 1.92e-85 246
Msa1418770 AT5G18380.2 87.200 125 16 0 2 126 8 132 3.84e-79 230
Msa1418770 AT5G18380.3 87.037 108 14 0 2 109 8 115 1.18e-67 201

Find 41 sgRNAs with CRISPR-Local

Find 39 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAATCTAACGATAAGATTTC+TGG 0.210396 tig0024639:+74645 None:intergenic
ACAGAGTAAGAAGGAGATTA+AGG 0.258952 tig0024639:-74762 Msa1418770:CDS
CGCTGTGAGCCTAAGAAGTT+TGG 0.359075 tig0024639:-74692 Msa1418770:CDS
CTGCAACAGCGTTCTTCTTC+CGG 0.375769 tig0024639:+75026 None:intergenic
TCAGAGTATTGCCAAGGCTT+TGG 0.384487 tig0024639:-74813 Msa1418770:CDS
GCCTGAGATTCTTCGTTTCA+AGG 0.392228 tig0024639:-74939 Msa1418770:CDS
CCACGACCACCAAACTTCTT+AGG 0.436009 tig0024639:+74683 None:intergenic
TACGAGCCAATCCTCCTTCT+CGG 0.440837 tig0024639:-74914 Msa1418770:CDS
TCTGACGAATGGCATAGATC+TGG 0.445614 tig0024639:+74831 None:intergenic
ATGGAACAGGTGCAATGCTT+TGG 0.453443 tig0024639:-75049 Msa1418770:CDS
GCCTTGAAACGAAGAATCTC+AGG 0.478888 tig0024639:+74938 None:intergenic
GAAGCTCAGCAAGATGGAAC+AGG 0.488449 tig0024639:-75062 None:intergenic
CGCGGTCTCATCAAGATCAA+TGG 0.494184 tig0024639:-74983 Msa1418770:CDS
TCATATCAACTCCGGCGAAA+CGG 0.494924 tig0024639:+74885 None:intergenic
TCAGGCTCCACGAGTTCGAT+TGG 0.496656 tig0024639:+74956 None:intergenic
GTGGTCGTGGTGCTCGTGCT+AGG 0.502073 tig0024639:-74670 Msa1418770:CDS
CTCGGAAGACACCGTTTCGC+CGG 0.504037 tig0024639:-74896 Msa1418770:CDS
GTTTCGCCGGAGTTGATATG+AGG 0.511168 tig0024639:-74883 Msa1418770:CDS
CCTAAGAAGTTTGGTGGTCG+TGG 0.511400 tig0024639:-74683 Msa1418770:CDS
AGGATCCGTGTGAAGGGTGG+AGG 0.521481 tig0024639:-74863 Msa1418770:CDS
CTGACGAATGGCATAGATCT+GGG 0.534090 tig0024639:+74832 None:intergenic
TGTTGATGAACAGAGTAAGA+AGG 0.534508 tig0024639:-74771 Msa1418770:CDS
ATAATAAGCAACCAAAGCCT+TGG 0.545151 tig0024639:+74802 None:intergenic
AACAGGTGCAATGCTTTGGC+CGG 0.546867 tig0024639:-75045 Msa1418770:CDS
AACGGTGTCTTCCGAGAAGG+AGG 0.549270 tig0024639:+74903 None:intergenic
TGTGAGCCTAAGAAGTTTGG+TGG 0.558448 tig0024639:-74689 Msa1418770:CDS
ACCCACTGCAAGAGAGGTCG+CGG 0.562945 tig0024639:-75001 Msa1418770:CDS
GCAGTTACCCACTGCAAGAG+AGG 0.580125 tig0024639:-75007 Msa1418770:CDS
TGTCTTCCGAGAAGGAGGAT+TGG 0.582501 tig0024639:+74908 None:intergenic
TGGCTGTCCAATCGAACTCG+TGG 0.587642 tig0024639:-74963 Msa1418770:CDS
TTCTTAGGCTCACAGCGTCT+AGG 0.590494 tig0024639:+74698 None:intergenic
CTTGGCAATACTCTGACGAA+TGG 0.607688 tig0024639:+74820 None:intergenic
ACCGCGACCTCTCTTGCAGT+GGG 0.618077 tig0024639:+75000 None:intergenic
CATTCGTCAGAGTATTGCCA+AGG 0.620290 tig0024639:-74819 Msa1418770:CDS
CGAAACGGTGTCTTCCGAGA+AGG 0.622095 tig0024639:+74900 None:intergenic
GACCGCGACCTCTCTTGCAG+TGG 0.624398 tig0024639:+74999 None:intergenic
TGATATGAGGATCCGTGTGA+AGG 0.626071 tig0024639:-74870 Msa1418770:CDS
ATGAGGATCCGTGTGAAGGG+TGG 0.634725 tig0024639:-74866 Msa1418770:CDS
TGTGACCTCCACCCTTCACA+CGG 0.645312 tig0024639:+74858 None:intergenic
ACGGATCCTCATATCAACTC+CGG 0.648153 tig0024639:+74877 None:intergenic
GATATGAGGATCCGTGTGAA+GGG 0.662094 tig0024639:-74869 Msa1418770:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
ATAATAAGCAACCAAAGCCT+TGG + tig0024639:74899-74918 None:intergenic 35.0%
! TGTTGATGAACAGAGTAAGA+AGG - tig0024639:74927-74946 Msa1418770:CDS 35.0%
ACAGAGTAAGAAGGAGATTA+AGG - tig0024639:74936-74955 Msa1418770:CDS 35.0%
ATGGAACAGGTGCAATGCTT+TGG - tig0024639:74649-74668 Msa1418770:CDS 45.0%
GCCTGAGATTCTTCGTTTCA+AGG - tig0024639:74759-74778 Msa1418770:CDS 45.0%
GCCTTGAAACGAAGAATCTC+AGG + tig0024639:74763-74782 None:intergenic 45.0%
TCATATCAACTCCGGCGAAA+CGG + tig0024639:74816-74835 None:intergenic 45.0%
ACGGATCCTCATATCAACTC+CGG + tig0024639:74824-74843 None:intergenic 45.0%
TGATATGAGGATCCGTGTGA+AGG - tig0024639:74828-74847 Msa1418770:CDS 45.0%
GATATGAGGATCCGTGTGAA+GGG - tig0024639:74829-74848 Msa1418770:CDS 45.0%
CTGACGAATGGCATAGATCT+GGG + tig0024639:74869-74888 None:intergenic 45.0%
TCTGACGAATGGCATAGATC+TGG + tig0024639:74870-74889 None:intergenic 45.0%
CTTGGCAATACTCTGACGAA+TGG + tig0024639:74881-74900 None:intergenic 45.0%
!! CATTCGTCAGAGTATTGCCA+AGG - tig0024639:74879-74898 Msa1418770:CDS 45.0%
!! TCAGAGTATTGCCAAGGCTT+TGG - tig0024639:74885-74904 Msa1418770:CDS 45.0%
TGTGAGCCTAAGAAGTTTGG+TGG - tig0024639:75009-75028 Msa1418770:CDS 45.0%
AACAGGTGCAATGCTTTGGC+CGG - tig0024639:74653-74672 Msa1418770:CDS 50.0%
CTGCAACAGCGTTCTTCTTC+CGG + tig0024639:74675-74694 None:intergenic 50.0%
CGCGGTCTCATCAAGATCAA+TGG - tig0024639:74715-74734 Msa1418770:CDS 50.0%
TACGAGCCAATCCTCCTTCT+CGG - tig0024639:74784-74803 Msa1418770:CDS 50.0%
TGTCTTCCGAGAAGGAGGAT+TGG + tig0024639:74793-74812 None:intergenic 50.0%
!! GTTTCGCCGGAGTTGATATG+AGG - tig0024639:74815-74834 Msa1418770:CDS 50.0%
TTCTTAGGCTCACAGCGTCT+AGG + tig0024639:75003-75022 None:intergenic 50.0%
CGCTGTGAGCCTAAGAAGTT+TGG - tig0024639:75006-75025 Msa1418770:CDS 50.0%
CCACGACCACCAAACTTCTT+AGG + tig0024639:75018-75037 None:intergenic 50.0%
! CCTAAGAAGTTTGGTGGTCG+TGG - tig0024639:75015-75034 Msa1418770:CDS 50.0%
GCAGTTACCCACTGCAAGAG+AGG - tig0024639:74691-74710 Msa1418770:CDS 55.0%
TGGCTGTCCAATCGAACTCG+TGG - tig0024639:74735-74754 Msa1418770:CDS 55.0%
TCAGGCTCCACGAGTTCGAT+TGG + tig0024639:74745-74764 None:intergenic 55.0%
AACGGTGTCTTCCGAGAAGG+AGG + tig0024639:74798-74817 None:intergenic 55.0%
CGAAACGGTGTCTTCCGAGA+AGG + tig0024639:74801-74820 None:intergenic 55.0%
ATGAGGATCCGTGTGAAGGG+TGG - tig0024639:74832-74851 Msa1418770:CDS 55.0%
TGTGACCTCCACCCTTCACA+CGG + tig0024639:74843-74862 None:intergenic 55.0%
ACCCACTGCAAGAGAGGTCG+CGG - tig0024639:74697-74716 Msa1418770:CDS 60.0%
ACCGCGACCTCTCTTGCAGT+GGG + tig0024639:74701-74720 None:intergenic 60.0%
CTCGGAAGACACCGTTTCGC+CGG - tig0024639:74802-74821 Msa1418770:CDS 60.0%
AGGATCCGTGTGAAGGGTGG+AGG - tig0024639:74835-74854 Msa1418770:CDS 60.0%
GACCGCGACCTCTCTTGCAG+TGG + tig0024639:74702-74721 None:intergenic 65.0%
GTGGTCGTGGTGCTCGTGCT+AGG - tig0024639:75028-75047 Msa1418770:CDS 65.0%
Chromosome Type Strat End Strand Name
tig0024639 gene 74649 75071 74649 ID=Msa1418770;Name=Msa1418770
tig0024639 mRNA 74649 75071 74649 ID=Msa1418770-mRNA-1;Parent=Msa1418770;Name=Msa1418770-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0|-1|0|1|-1|1|1|0|140
tig0024639 exon 74649 75071 74649 ID=Msa1418770-mRNA-1:exon:492;Parent=Msa1418770-mRNA-1
tig0024639 CDS 74649 75071 74649 ID=Msa1418770-mRNA-1:cds;Parent=Msa1418770-mRNA-1
Gene Sequence

>Msa1418770

ATGGAACAGGTGCAATGCTTTGGCCGGAAGAAGAACGCTGTTGCAGTTACCCACTGCAAGAGAGGTCGCGGTCTCATCAAGATCAATGGCTGTCCAATCGAACTCGTGGAGCCTGAGATTCTTCGTTTCAAGGCATACGAGCCAATCCTCCTTCTCGGAAGACACCGTTTCGCCGGAGTTGATATGAGGATCCGTGTGAAGGGTGGAGGTCACACTTCCCAGATCTATGCCATTCGTCAGAGTATTGCCAAGGCTTTGGTTGCTTATTATCAGAAATATGTTGATGAACAGAGTAAGAAGGAGATTAAGGATATTCTTGTTCGTTATGATCGTACTCTTCTTGTTGCTGATCCTAGACGCTGTGAGCCTAAGAAGTTTGGTGGTCGTGGTGCTCGTGCTAGGTTCCAGAAATCTTATCGTTAG

Protein sequence

>Msa1418770

MEQVQCFGRKKNAVAVTHCKRGRGLIKINGCPIELVEPEILRFKAYEPILLLGRHRFAGVDMRIRVKGGGHTSQIYAIRQSIAKALVAYYQKYVDEQSKKEIKDILVRYDRTLLVADPRRCEPKKFGGRGARARFQKSYR*