AlfalfaGEDB | Msa0097940

M. sativa cultivar zhongmu-4 / Msa0097940

KEGG: -
GO: GO:0000041 GO:0003674 GO:0005215 GO:0005381 GO:0005384 GO:0005575 GO:0006810 GO:0006811 GO:0006812 GO:0006826 GO:0006828 GO:0006873 GO:0006875 GO:0006879 GO:0006880 GO:0008150 GO:0008324 GO:0009987 GO:0010035 GO:0010038 GO:0010039 GO:0015075 GO:0015318 GO:0016020 GO:0019725 GO:0022857 GO:0022890 GO:0030001 GO:0030003 GO:0030026 GO:0034220 GO:0034755 GO:0042221 GO:0042592 GO:0046873 GO:0046915 GO:0046916 GO:0048878 GO:0050801 GO:0050896 GO:0051179 GO:0051234 GO:0051235 GO:0051238 GO:0051641 GO:0051651 GO:0051716 GO:0055065 GO:0055071 GO:0055072 GO:0055076 GO:0055080 GO:0055082 GO:0055085 GO:0065007 GO:0065008 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071421 GO:0097577 GO:0098655 GO:0098660 GO:0098662 GO:0098771
PFAM: PF01988.22

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0097940	XP_003592125.1	96.396	222	2	2	1	221	1	217	5.16e-148	421

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0097940	sp\|Q9M2C0\|VITH4_ARATH	66.516	221	51	4	1	221	1	198	5.26e-100	291

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0097940	G7ID02	96.396	222	2	2	1	221	1	217	2.46e-148	421

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0050760	Msa0097940	0.805678	1.188600e-49	-8.615850e-47
Msa0097940	Msa0140910	0.807558	4.765761e-50	-8.615850e-47
Msa0097940	Msa0449760	0.828423	9.063076e-55	-8.615850e-47
Msa0097940	Msa0578970	0.801407	9.136064e-49	-8.615850e-47
Msa0097940	Msa0599580	0.831113	2.006847e-55	-8.615850e-47
Msa0097940	Msa0610590	0.804777	1.835330e-49	-8.615850e-47
Msa0097940	Msa0651190	0.812069	5.102963e-51	-8.615850e-47
Msa0097940	Msa0667890	0.804742	1.866867e-49	-8.615850e-47
Msa0097940	Msa0718470	0.801490	8.784368e-49	-8.615850e-47
Msa0097940	Msa0731710	0.821348	4.235278e-53	-8.615850e-47
Msa0097940	Msa0835730	0.808624	2.825938e-50	-8.615850e-47
Msa0097940	Msa0846810	0.814693	1.352856e-51	-8.615850e-47
Msa0097940	Msa1080790	0.822063	2.894398e-53	-8.615850e-47
Msa0097940	Msa1118930	0.821569	3.765062e-53	-8.615850e-47
Msa0097940	Msa1233500	0.809686	1.673404e-50	-8.615850e-47
Msa0097940	Msa1316560	-0.806171	9.365630e-50	-8.615850e-47
Msa0097940	Msa1440600	0.805996	1.019265e-49	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0097940	MtrunA17_Chr1g0202031	96.396	222	2	2	1	221	1	217	4.74e-152	421
Msa0097940	MtrunA17_Chr6g0463641	79.630	216	37	3	6	221	2	210	2.45e-107	307
Msa0097940	MtrunA17_Chr4g0050821	65.550	209	71	1	13	221	17	224	1.45e-85	253
Msa0097940	MtrunA17_Chr4g0050841	66.832	202	66	1	20	221	24	224	1.65e-85	253
Msa0097940	MtrunA17_Chr4g0050831	66.832	202	66	1	20	221	24	224	3.51e-85	251
Msa0097940	MtrunA17_Chr4g0050811	61.611	211	73	2	15	221	33	239	6.80e-84	249
Msa0097940	MtrunA17_Chr6g0479471	69.312	189	48	2	28	216	29	207	3.26e-79	236
Msa0097940	MtrunA17_Chr2g0278621	69.312	189	48	2	28	216	29	207	3.55e-79	236
Msa0097940	MtrunA17_Chr4g0050851	57.870	216	84	3	9	221	20	231	6.49e-71	216

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0097940	AT3G43660.1	66.516	221	51	4	1	221	1	198	5.34e-101	291
Msa0097940	AT3G43630.1	73.039	204	41	4	18	218	2	194	4.68e-94	273
Msa0097940	AT1G21140.1	70.673	208	48	4	18	221	2	200	7.42e-94	273
Msa0097940	AT1G76800.1	71.707	205	48	1	17	221	2	196	1.82e-92	270
Msa0097940	AT3G25190.1	59.906	212	80	2	14	221	7	217	1.98e-68	209

Find 43 sgRNAs with CRISPR-Local

Find 57 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GGTTAGGGTAGGTGTTGTTT+TGG	0.266162	1_3:+3825343	Msa0097940:CDS
ATCTTTCATTAAAGATTATA+AGG	0.270047	1_3:+3825322	Msa0097940:CDS
ATTGCTATGGCCATAACTTT+TGG	0.287767	1_3:+3825467	Msa0097940:CDS
TTGGTTAAGGGATGAATTAT+TGG	0.297757	1_3:-3824865	None:intergenic
GGTTGGTTGGGTGCAGTGTT+GGG	0.338405	1_3:+3825395	Msa0097940:CDS
TCATTAAAGATTATAAGGTT+AGG	0.355897	1_3:+3825327	Msa0097940:CDS
TTTGGACTAACCAAGTTGAT+TGG	0.369529	1_3:+3825485	Msa0097940:CDS
TGGTTGGTTGGGTGCAGTGT+TGG	0.376731	1_3:+3825394	Msa0097940:CDS
TTTGGTTGTGTTTGGTTGGT+TGG	0.383193	1_3:+3825382	Msa0097940:CDS
GCTGCTGCTTGTAAAGGATT+TGG	0.384499	1_3:-3825248	None:intergenic
CTTGGTTAGTCCAAAAGTTA+TGG	0.395661	1_3:-3825477	None:intergenic
TTGGTTGTGTTTGGTTGGTT+GGG	0.399852	1_3:+3825383	Msa0097940:CDS
CAGTGGTAAGGTCTTGTTTG+AGG	0.403097	1_3:+3825426	Msa0097940:CDS
ATTCATCGCTTTCGATCTCT+AGG	0.414680	1_3:-3824935	None:intergenic
AGTGGTAAGGTCTTGTTTGA+GGG	0.418892	1_3:+3825427	Msa0097940:CDS
TTCAACAGCATCACTAATGA+TGG	0.422094	1_3:+3825022	Msa0097940:CDS
GTTGGGTGCAGTGTTGGGTA+AGG	0.428457	1_3:+3825400	Msa0097940:CDS
TCTTGTTTGAGGGTCTTACT+TGG	0.431071	1_3:+3825437	Msa0097940:CDS
ATAAAAGCAATGATCTTGTC+AGG	0.437497	1_3:+3825068	Msa0097940:CDS
CATTAAAGATTATAAGGTTA+GGG	0.441558	1_3:+3825328	Msa0097940:CDS
TGTTTGAGGGTCTTACTTGG+AGG	0.451549	1_3:+3825440	Msa0097940:CDS
ACCAAGTTGATTGGGTCAAG+TGG	0.451627	1_3:+3825494	Msa0097940:CDS
TTGGACTAACCAAGTTGATT+GGG	0.452121	1_3:+3825486	Msa0097940:CDS
TTGCTTTGGTTGTGTTTGGT+TGG	0.457210	1_3:+3825378	Msa0097940:CDS
ACTTGGAGGTTGGATTGCTA+TGG	0.458097	1_3:+3825454	Msa0097940:CDS
TGAGGGTCTTACTTGGAGGT+TGG	0.468585	1_3:+3825444	Msa0097940:CDS
CCCACTTGACCCAATCAACT+TGG	0.477807	1_3:-3825495	None:intergenic
AAAAGAGACAATATTGAAAG+AGG	0.486922	1_3:+3825182	Msa0097940:CDS
GGTGCATGTAGCATGGCAAT+AGG	0.539596	1_3:+3825110	Msa0097940:CDS
GAAGCTGCTGCTGCTTGTAA+AGG	0.540279	1_3:-3825254	None:intergenic
ATGAAAGATGCTGCAAGAAG+TGG	0.543716	1_3:-3825308	None:intergenic
TGGGTAAGGCACCAGTGGTA+AGG	0.548709	1_3:+3825414	Msa0097940:CDS
AAAGATTATAAGGTTAGGGT+AGG	0.554719	1_3:+3825332	Msa0097940:CDS
AAGAGGTAATAATATTGAAG+AGG	0.573020	1_3:+3825199	Msa0097940:CDS
TTCATCGCTTTCGATCTCTA+GGG	0.576335	1_3:-3824934	None:intergenic
CCAAGTTGATTGGGTCAAGT+GGG	0.582206	1_3:+3825495	Msa0097940:CDS
ATCACAATGGCTACGTGCCG+CGG	0.590039	1_3:+3824974	Msa0097940:CDS
GCATCACTAATGATGGGTGT+AGG	0.595742	1_3:+3825029	Msa0097940:CDS
CTCAAACAAGACCTTACCAC+TGG	0.603707	1_3:-3825425	None:intergenic
ATCATTAGCTCCTAAAACCG+CGG	0.609497	1_3:-3824991	None:intergenic
TCAACAGCATCACTAATGAT+GGG	0.621364	1_3:+3825023	Msa0097940:CDS
AGTGTTGGGTAAGGCACCAG+TGG	0.645814	1_3:+3825409	Msa0097940:CDS
TGTAGCTGGTGCATGTAGCA+TGG	0.650697	1_3:+3825103	Msa0097940:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATCTTTCATTAAAGATTATA+AGG	+	chr1_3:3825322-3825341	Msa0097940:CDS	15.0%
!!!	TACAAATTTTGTTTGGTTAA+GGG	-	chr1_3:3824880-3824899	None:intergenic	20.0%
!!!	TTTGATGTACAAATTTTGTT+TGG	-	chr1_3:3824887-3824906	None:intergenic	20.0%
!!	ATTATTCAAAAAGATCACAA+TGG	+	chr1_3:3824961-3824980	Msa0097940:CDS	20.0%
!!	TCATTAAAGATTATAAGGTT+AGG	+	chr1_3:3825327-3825346	Msa0097940:CDS	20.0%
!!	CATTAAAGATTATAAGGTTA+GGG	+	chr1_3:3825328-3825347	Msa0097940:CDS	20.0%
!!!	GTACAAATTTTGTTTGGTTA+AGG	-	chr1_3:3824881-3824900	None:intergenic	25.0%
!	AAAAGAGACAATATTGAAAG+AGG	+	chr1_3:3825182-3825201	Msa0097940:CDS	25.0%
!	AAGAGGTAATAATATTGAAG+AGG	+	chr1_3:3825199-3825218	Msa0097940:CDS	25.0%
	TTGGTTAAGGGATGAATTAT+TGG	-	chr1_3:3824868-3824887	None:intergenic	30.0%
	ATAAAAGCAATGATCTTGTC+AGG	+	chr1_3:3825068-3825087	Msa0097940:CDS	30.0%
!!	TCAGCTTTAGCATTTTCAAT+TGG	+	chr1_3:3825275-3825294	Msa0097940:CDS	30.0%
	AAAGATTATAAGGTTAGGGT+AGG	+	chr1_3:3825332-3825351	Msa0097940:CDS	30.0%
	TTCAACAGCATCACTAATGA+TGG	+	chr1_3:3825022-3825041	Msa0097940:CDS	35.0%
	TCAACAGCATCACTAATGAT+GGG	+	chr1_3:3825023-3825042	Msa0097940:CDS	35.0%
!	AGCATTTTCAATTGGTGCAA+TGG	+	chr1_3:3825283-3825302	Msa0097940:CDS	35.0%
!	AGTTTTGCTTTGGTTGTGTT+TGG	+	chr1_3:3825374-3825393	Msa0097940:CDS	35.0%
!	ATTGCTATGGCCATAACTTT+TGG	+	chr1_3:3825467-3825486	Msa0097940:CDS	35.0%
!	CTTGGTTAGTCCAAAAGTTA+TGG	-	chr1_3:3825480-3825499	None:intergenic	35.0%
!!	TTTGGACTAACCAAGTTGAT+TGG	+	chr1_3:3825485-3825504	Msa0097940:CDS	35.0%
!!	TTGGACTAACCAAGTTGATT+GGG	+	chr1_3:3825486-3825505	Msa0097940:CDS	35.0%
!	GGGTTGCATTTTGTTGATCA+TGG	-	chr1_3:3824917-3824936	None:intergenic	40.0%
	TTCATCGCTTTCGATCTCTA+GGG	-	chr1_3:3824937-3824956	None:intergenic	40.0%
	ATTCATCGCTTTCGATCTCT+AGG	-	chr1_3:3824938-3824957	None:intergenic	40.0%
	ATCATTAGCTCCTAAAACCG+CGG	-	chr1_3:3824994-3825013	None:intergenic	40.0%
!	ATGATCTTGTCAGGTTTTGC+TGG	+	chr1_3:3825077-3825096	Msa0097940:CDS	40.0%
	ATGAAAGATGCTGCAAGAAG+TGG	-	chr1_3:3825311-3825330	None:intergenic	40.0%
!!!	GTTAGGGTAGGTGTTGTTTT+GGG	+	chr1_3:3825344-3825363	Msa0097940:CDS	40.0%
	TTGCTTTGGTTGTGTTTGGT+TGG	+	chr1_3:3825378-3825397	Msa0097940:CDS	40.0%
	TTTGGTTGTGTTTGGTTGGT+TGG	+	chr1_3:3825382-3825401	Msa0097940:CDS	40.0%
!	TTGGTTGTGTTTGGTTGGTT+GGG	+	chr1_3:3825383-3825402	Msa0097940:CDS	40.0%
!!	AGTGGTAAGGTCTTGTTTGA+GGG	+	chr1_3:3825427-3825446	Msa0097940:CDS	40.0%
!	TCTTGTTTGAGGGTCTTACT+TGG	+	chr1_3:3825437-3825456	Msa0097940:CDS	40.0%
!!!	GCGGTTTTAGGAGCTAATGA+TGG	+	chr1_3:3824993-3825012	Msa0097940:CDS	45.0%
!!	GCATCACTAATGATGGGTGT+AGG	+	chr1_3:3825029-3825048	Msa0097940:CDS	45.0%
!	GCTGCTGCTTGTAAAGGATT+TGG	-	chr1_3:3825251-3825270	None:intergenic	45.0%
!!!	GGTTAGGGTAGGTGTTGTTT+TGG	+	chr1_3:3825343-3825362	Msa0097940:CDS	45.0%
!!!	GGGAGCAGTTAGTTTTGCTT+TGG	+	chr1_3:3825364-3825383	Msa0097940:CDS	45.0%
	CTCAAACAAGACCTTACCAC+TGG	-	chr1_3:3825428-3825447	None:intergenic	45.0%
!!	CAGTGGTAAGGTCTTGTTTG+AGG	+	chr1_3:3825426-3825445	Msa0097940:CDS	45.0%
	TGTTTGAGGGTCTTACTTGG+AGG	+	chr1_3:3825440-3825459	Msa0097940:CDS	45.0%
	ACTTGGAGGTTGGATTGCTA+TGG	+	chr1_3:3825454-3825473	Msa0097940:CDS	45.0%
!!	ACCAAGTTGATTGGGTCAAG+TGG	+	chr1_3:3825494-3825513	Msa0097940:CDS	45.0%
!!	CCAAGTTGATTGGGTCAAGT+GGG	+	chr1_3:3825495-3825514	Msa0097940:CDS	45.0%
!	GGTTTTGCTGGACTTGTAGC+TGG	+	chr1_3:3825089-3825108	Msa0097940:CDS	50.0%
	TGTAGCTGGTGCATGTAGCA+TGG	+	chr1_3:3825103-3825122	Msa0097940:CDS	50.0%
	GGTGCATGTAGCATGGCAAT+AGG	+	chr1_3:3825110-3825129	Msa0097940:CDS	50.0%
!	GAAGCTGCTGCTGCTTGTAA+AGG	-	chr1_3:3825257-3825276	None:intergenic	50.0%
	TGAGGGTCTTACTTGGAGGT+TGG	+	chr1_3:3825444-3825463	Msa0097940:CDS	50.0%
	CCCACTTGACCCAATCAACT+TGG	-	chr1_3:3825498-3825517	None:intergenic	50.0%
	ATCACAATGGCTACGTGCCG+CGG	+	chr1_3:3824974-3824993	Msa0097940:CDS	55.0%
!	TGGTTGGTTGGGTGCAGTGT+TGG	+	chr1_3:3825394-3825413	Msa0097940:CDS	55.0%
!	GGTTGGTTGGGTGCAGTGTT+GGG	+	chr1_3:3825395-3825414	Msa0097940:CDS	55.0%
!	GTTGGGTGCAGTGTTGGGTA+AGG	+	chr1_3:3825400-3825419	Msa0097940:CDS	55.0%
!	AGTGTTGGGTAAGGCACCAG+TGG	+	chr1_3:3825409-3825428	Msa0097940:CDS	55.0%
!	TGGGTAAGGCACCAGTGGTA+AGG	+	chr1_3:3825414-3825433	Msa0097940:CDS	55.0%
!	TGGCTACGTGCCGCGGTTTT+AGG	+	chr1_3:3824981-3825000	Msa0097940:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr1_3	gene	3824858	3825523	3824858	ID=Msa0097940;Name=Msa0097940
chr1_3	mRNA	3824858	3825523	3824858	ID=Msa0097940-mRNA-1;Parent=Msa0097940;Name=Msa0097940-mRNA-1;_AED=0.06;_eAED=0.06;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|221
chr1_3	exon	3824858	3825523	3824858	ID=Msa0097940-mRNA-1:exon:1221;Parent=Msa0097940-mRNA-1
chr1_3	CDS	3824858	3825523	3824858	ID=Msa0097940-mRNA-1:cds;Parent=Msa0097940-mRNA-1

Gene Sequence

>Msa0097940

ATGGAATCCAATAATTCATCCCTTAACCAAACAAAATTTGTACATCAAAACAATGACCATGATCAACAAAATGCAACCCTAGAGATCGAAAGCGATGAATTTGATTATTCAAAAAGATCACAATGGCTACGTGCCGCGGTTTTAGGAGCTAATGATGGATTAGTTTCAACAGCATCACTAATGATGGGTGTAGGAGCAGTGAAACAAGACATAAAAGCAATGATCTTGTCAGGTTTTGCTGGACTTGTAGCTGGTGCATGTAGCATGGCAATAGGTGAGTTTGTATCTGTTTATTCACAACTTGACATTGAAATTGCTCAATTAAAAAGAGACAATATTGAAAGAGGTAATAATATTGAAGAGGAAGATGTTATTGAGAAAGAAAGTTTGCCAAATCCTTTACAAGCAGCAGCAGCTTCAGCTTTAGCATTTTCAATTGGTGCAATGGTGCCACTTCTTGCAGCATCTTTCATTAAAGATTATAAGGTTAGGGTAGGTGTTGTTTTGGGAGCAGTTAGTTTTGCTTTGGTTGTGTTTGGTTGGTTGGGTGCAGTGTTGGGTAAGGCACCAGTGGTAAGGTCTTGTTTGAGGGTCTTACTTGGAGGTTGGATTGCTATGGCCATAACTTTTGGACTAACCAAGTTGATTGGGTCAAGTGGGCTATAG

Protein sequence

>Msa0097940

MESNNSSLNQTKFVHQNNDHDQQNATLEIESDEFDYSKRSQWLRAAVLGANDGLVSTASLMMGVGAVKQDIKAMILSGFAGLVAGACSMAIGEFVSVYSQLDIEIAQLKRDNIERGNNIEEEDVIEKESLPNPLQAAAASALAFSIGAMVPLLAASFIKDYKVRVGVVLGAVSFALVVFGWLGAVLGKAPVVRSCLRVLLGGWIAMAITFGLTKLIGSSGL*