AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0151840

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0151840 MtrunA17_Chr1g0160501 99.422 173 1 0 1 173 1 173 2.26e-127 354
Msa0151840 MtrunA17_Chr1g0197081 59.627 161 64 1 1 160 42 202 7.16e-71 213
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0151840 AT1G30110.2 77.714 175 37 1 1 173 1 175 5.06e-101 288
Msa0151840 AT1G30110.1 77.714 175 37 1 1 173 1 175 5.06e-101 288
Msa0151840 AT3G10620.1 57.764 161 67 1 1 160 56 216 2.00e-68 207
Msa0151840 AT5G06340.1 56.250 160 69 1 1 159 55 214 1.57e-62 192

Find 77 sgRNAs with CRISPR-Local

Find 165 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATTTCCACTCTGCAAATTC+TGG 0.093641 1_4:+14200510 None:intergenic
CCCGGTTTAGAAAATAAATT+TGG 0.192294 1_4:+14199899 None:intergenic
TTCTCTAATAGCAGCAGATT+TGG 0.244965 1_4:+14201306 None:intergenic
CTCAGTAAGTGAAATGGATT+TGG 0.268607 1_4:+14200015 None:intergenic
AACACTCAACTATTTGTAAA+TGG 0.270794 1_4:-14199983 Msa0151840:three_prime_UTR
TAAACTTCTTCTTCTCTCTA+AGG 0.276839 1_4:-14202506 Msa0151840:five_prime_UTR
CAGAATTTGCAGAGTGGAAA+TGG 0.298244 1_4:-14200509 Msa0151840:CDS
GGCTAAAGTCAATCGTCTTT+GGG 0.307296 1_4:-14200934 Msa0151840:CDS
TTCGCGTACATCTGTGCCTT+TGG 0.307652 1_4:-14200183 Msa0151840:three_prime_UTR
TAGCTGTTACTTGTTATTGT+TGG 0.319130 1_4:+14202477 None:intergenic
CAAAATTGTTCCTTGTACTT+AGG 0.322157 1_4:+14199808 None:intergenic
ACCTGCTCAATAACTTCTTC+AGG 0.335949 1_4:+14200480 None:intergenic
AGGCTAAAGTCAATCGTCTT+TGG 0.336576 1_4:-14200935 Msa0151840:CDS
AGTGAAATTAGATAAGAAAA+TGG 0.345012 1_4:+14200242 None:intergenic
GTTGCATTTCCTTGAAAGTA+AGG 0.354258 1_4:+14200324 None:intergenic
ACCTTCAAGCCTTACTTTCA+AGG 0.360232 1_4:-14200333 Msa0151840:CDS
GTGCATTACAGGTTGATAAA+TGG 0.375642 1_4:-14200986 Msa0151840:intron
TCTCTAATAGCAGCAGATTT+GGG 0.380677 1_4:+14201307 None:intergenic
TCGCGTACATCTGTGCCTTT+GGG 0.385910 1_4:-14200182 Msa0151840:three_prime_UTR
GCTTCCAGATTGAATGTTCC+AGG 0.385919 1_4:-14201494 Msa0151840:CDS
ATTTCCACTCTGCAAATTCT+GGG 0.388442 1_4:+14200511 None:intergenic
GGTTGTTACAACTTCTTCAT+AGG 0.389048 1_4:+14200353 None:intergenic
TTGAAATGACATCATGTTTG+AGG 0.392954 1_4:-14199715 Msa0151840:three_prime_UTR
GGTAGTTAGAACTTTCCATT+AGG 0.400278 1_4:-14199958 Msa0151840:three_prime_UTR
TCCATTTCACTTACTGAGGT+TGG 0.401653 1_4:-14200010 Msa0151840:three_prime_UTR
AGAGAACTTCGAGAAGAAAC+TGG 0.413791 1_4:-14201289 Msa0151840:CDS
TACAAGAAGCAAGTACCTAA+TGG 0.421481 1_4:+14199943 None:intergenic
TCTCATCAATTCAGATGATC+AGG 0.421614 1_4:-14202406 Msa0151840:intron
TTTCAAAGAACATGTTAGAA+TGG 0.423755 1_4:+14199732 None:intergenic
TCCTTGAAAGTAAGGCTTGA+AGG 0.425267 1_4:+14200332 None:intergenic
TCCAACCTCAGTAAGTGAAA+TGG 0.425402 1_4:+14200009 None:intergenic
TGAGGCATTTGCCATGCTCC+TGG 0.426678 1_4:+14201476 None:intergenic
TGCTCCTGGAACATTCAATC+TGG 0.446702 1_4:+14201490 None:intergenic
AGGAGCATGGCAAATGCCTC+AGG 0.465802 1_4:-14201474 Msa0151840:intron
GAACATGTTAGAATGGTTGA+TGG 0.472895 1_4:+14199739 None:intergenic
AAAATATCGAGCTTGAAATC+CGG 0.488170 1_4:+14200144 None:intergenic
CCTGCTCAATAACTTCTTCA+GGG 0.490006 1_4:+14200481 None:intergenic
CCCTGAAGAAGTTATTGAGC+AGG 0.490394 1_4:-14200481 Msa0151840:intron
GTGTTGACAGTGCGAACGTA+TGG 0.490885 1_4:-14200057 Msa0151840:three_prime_UTR
GGCATGGACAGGCACAGAAA+TGG 0.494440 1_4:-14200902 Msa0151840:intron
AGAATTTGCAGAGTGGAAAT+GGG 0.503598 1_4:-14200508 Msa0151840:CDS
TGGGTCAAAATCAGACAATC+CGG 0.506184 1_4:-14200163 Msa0151840:three_prime_UTR
GTTACAACTTCTTCATAGGT+AGG 0.511880 1_4:+14200357 None:intergenic
GGGAGGAGAATGGCATGGAC+AGG 0.513397 1_4:-14200913 Msa0151840:CDS
ATGCTTGAAATTTGTAGCTA+CGG 0.521900 1_4:+14200205 None:intergenic
ATGAGACAGACACCAACGTT+AGG 0.524807 1_4:+14202423 None:intergenic
ATCGTCTTTGGGGAGGAGAA+TGG 0.528661 1_4:-14200923 Msa0151840:CDS
TTGACTTTAGCCTTCACAGC+AGG 0.532701 1_4:+14200945 None:intergenic
TTGTTTATAACTTAAGTACA+AGG 0.537609 1_4:-14199788 Msa0151840:three_prime_UTR
TGACTTCCCTCCTGCTGTGA+AGG 0.542301 1_4:-14200955 Msa0151840:CDS
CTCAACTATTTGTAAATGGA+TGG 0.543339 1_4:-14199979 Msa0151840:three_prime_UTR
CAAATCCATTTCACTTACTG+AGG 0.549203 1_4:-14200014 Msa0151840:three_prime_UTR
TCACAATCATCCTCGCAGCC+CGG 0.551358 1_4:+14199881 None:intergenic
ACTTTAGCCTTCACAGCAGG+AGG 0.558265 1_4:+14200948 None:intergenic
CAACACAAACACAACACAAG+TGG 0.567169 1_4:+14202595 None:intergenic
GATTGAATGTTCCAGGAGCA+TGG 0.573208 1_4:-14201487 Msa0151840:CDS
GAGGTGTGAATGATGAGTAC+TGG 0.579208 1_4:+14202569 None:intergenic
AGACAATGCAGAAAGCAAGC+AGG 0.590502 1_4:+14200096 None:intergenic
TTCGCACTGTCAACACGTGT+TGG 0.590594 1_4:+14200064 None:intergenic
TTCTGGGTCTGCTTCACCAG+TGG 0.598318 1_4:+14200527 None:intergenic
ATAGTATTGTATTGTGAATG+TGG 0.598793 1_4:+14202633 None:intergenic
TGACTTGATACCTAAGTACA+AGG 0.602640 1_4:-14199818 Msa0151840:three_prime_UTR
GCTAAAGTCAATCGTCTTTG+GGG 0.604526 1_4:-14200933 Msa0151840:CDS
GTGTTGTGTTAGAGATGATG+AGG 0.609871 1_4:+14202550 None:intergenic
GGTGAAATCAACTTAGCCAC+TGG 0.610366 1_4:-14200543 Msa0151840:CDS
AACAAGTAACAGCTAAGACA+TGG 0.618371 1_4:-14202469 Msa0151840:exon
CGAAATGTAAATCGACAAAG+TGG 0.620980 1_4:-14200296 Msa0151840:CDS
CTTTGGGGAGGAGAATGGCA+TGG 0.624852 1_4:-14200918 Msa0151840:CDS
GACAGACACCAACGTTAGGA+CGG 0.631134 1_4:+14202427 None:intergenic
CTTTAGCCTTCACAGCAGGA+GGG 0.649727 1_4:+14200949 None:intergenic
AAAGTCAATCGTCTTTGGGG+AGG 0.655166 1_4:-14200930 Msa0151840:CDS
ACGTTAGGACGGTAACCTTG+TGG 0.657862 1_4:+14202438 None:intergenic
GTCTGCTGAGATAATCGCTG+AGG 0.670608 1_4:-14201260 Msa0151840:intron
GTATAGATCTGTTGATGTCG+CGG 0.674346 1_4:-14199852 Msa0151840:three_prime_UTR
CAGACCCAGAATTTGCAGAG+TGG 0.693037 1_4:-14200515 Msa0151840:CDS
CAAGGTTACCGTCCTAACGT+TGG 0.707808 1_4:-14202435 Msa0151840:CDS
GACATGGAGAATCTTCCACA+AGG 0.720171 1_4:-14202453 Msa0151840:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTAATTGTTTATAGATTTT+AGG - chr1_4:14200182-14200201 Msa0151840:three_prime_UTR 10.0%
!!! ATTATTTAAGTTATTTGAAT+TGG + chr1_4:14200932-14200951 None:intergenic 10.0%
!! AAACAAAAGAAAAAAAAAGA+TGG + chr1_4:14199975-14199994 None:intergenic 15.0%
!!! ATTTTTCCATAATTCATTTT+TGG - chr1_4:14200242-14200261 Msa0151840:three_prime_UTR 15.0%
!!! ATGTTGTAAATTTAACTTAA+AGG - chr1_4:14200674-14200693 Msa0151840:intron 15.0%
!!! ATATTTTTTCCAGTAAAATT+TGG - chr1_4:14201638-14201657 Msa0151840:intron 15.0%
!! TGAAAATTTAGGTAAGAATA+TGG - chr1_4:14200029-14200048 Msa0151840:three_prime_UTR 20.0%
!!! TGTTGTTTGTAGATATTTTT+TGG - chr1_4:14200157-14200176 Msa0151840:three_prime_UTR 20.0%
!!! TGAAGTTGATTTATACTTTA+AGG + chr1_4:14200368-14200387 None:intergenic 20.0%
!!! TTTGTTTTTTCAGATATTTG+TGG - chr1_4:14200826-14200845 Msa0151840:intron 20.0%
!!! AATTTTTTGACTGTTCTTTT+GGG - chr1_4:14200980-14200999 Msa0151840:intron 20.0%
!!! ACAAAAGCATATATTAACAA+TGG - chr1_4:14201522-14201541 Msa0151840:intron 20.0%
!!! TCTTTTGTTATTAATAAGCA+TGG - chr1_4:14201677-14201696 Msa0151840:intron 20.0%
!! ATCAATTACGAACATTTAAT+AGG - chr1_4:14201749-14201768 Msa0151840:intron 20.0%
!! ATGAAGTTAAAAAAAGATGA+CGG - chr1_4:14201778-14201797 Msa0151840:intron 20.0%
!! AGTGAAATTAGATAAGAAAA+TGG + chr1_4:14202103-14202122 None:intergenic 20.0%
!!! TTTTCTTATCTAATTTCACT+TGG - chr1_4:14202103-14202122 Msa0151840:intron 20.0%
!! TTGTTTATAACTTAAGTACA+AGG - chr1_4:14202554-14202573 Msa0151840:five_prime_UTR 20.0%
! ATAGTATTGTATTGTGAATG+TGG + chr1_4:14199712-14199731 None:intergenic 25.0%
!! AACCTTTTCTGAATCTATTT+TGG - chr1_4:14200073-14200092 Msa0151840:three_prime_UTR 25.0%
! AACCAAAATAGATTCAGAAA+AGG + chr1_4:14200078-14200097 None:intergenic 25.0%
!!! TTCTGAATCTATTTTGGTTT+TGG - chr1_4:14200079-14200098 Msa0151840:three_prime_UTR 25.0%
!!! TTCCATAATTCATTTTTGGT+TGG - chr1_4:14200246-14200265 Msa0151840:three_prime_UTR 25.0%
! AACCAACCAAAAATGAATTA+TGG + chr1_4:14200251-14200270 None:intergenic 25.0%
!!! TTTGGTTGGTTTTCTATTTT+TGG - chr1_4:14200260-14200279 Msa0151840:three_prime_UTR 25.0%
!!! TTATATTTTTGTTAACCCTC+TGG - chr1_4:14200436-14200455 Msa0151840:intron 25.0%
!! TGATTAATGTTTTCCATCAA+AGG + chr1_4:14200562-14200581 None:intergenic 25.0%
! TACACTGTTATAGCATATAT+AGG + chr1_4:14200723-14200742 None:intergenic 25.0%
! GTATACTAGTACATAATATG+AGG + chr1_4:14200897-14200916 None:intergenic 25.0%
!!! CAATTTTTTGACTGTTCTTT+TGG - chr1_4:14200979-14200998 Msa0151840:intron 25.0%
!!! ATTTTTTGACTGTTCTTTTG+GGG - chr1_4:14200981-14201000 Msa0151840:intron 25.0%
! AACTAAATGCAGAAAAGAAA+AGG + chr1_4:14201119-14201138 None:intergenic 25.0%
! AAGAGAAACATTTAAGAATG+TGG + chr1_4:14201500-14201519 None:intergenic 25.0%
!! AAACACAAACCAAATTTTAC+TGG + chr1_4:14201650-14201669 None:intergenic 25.0%
!!! TTTGTTATTAATAAGCATGG+CGG - chr1_4:14201680-14201699 Msa0151840:intron 25.0%
! ACAAAGTCAGTATTTCTTAT+TGG - chr1_4:14201714-14201733 Msa0151840:intron 25.0%
!! GAAAAATAGATACTTGTTAC+AGG + chr1_4:14201905-14201924 None:intergenic 25.0%
! TGTTTGAAAAACTAATCACT+GGG - chr1_4:14201927-14201946 Msa0151840:intron 25.0%
! AACACTCAACTATTTGTAAA+TGG - chr1_4:14202359-14202378 Msa0151840:intron 25.0%
!!! GCCAAATTTATTTTCTAAAC+CGG - chr1_4:14202442-14202461 Msa0151840:CDS 25.0%
!!! CCAAATTTATTTTCTAAACC+GGG - chr1_4:14202443-14202462 Msa0151840:CDS 25.0%
! TTTCAAAGAACATGTTAGAA+TGG + chr1_4:14202613-14202632 None:intergenic 25.0%
TAAACTTCTTCTTCTCTCTA+AGG - chr1_4:14199836-14199855 Msa0151840:three_prime_UTR 30.0%
!! TAGCTGTTACTTGTTATTGT+TGG + chr1_4:14199868-14199887 None:intergenic 30.0%
GAATATGGTGTCTTAATCTA+AGG - chr1_4:14200044-14200063 Msa0151840:three_prime_UTR 30.0%
CACACTACATTAATATCTGA+CGG + chr1_4:14200129-14200148 None:intergenic 30.0%
! ATTTTAGGCTCTACTAAAGT+TGG - chr1_4:14200197-14200216 Msa0151840:three_prime_UTR 30.0%
!! GTTGATTTATACTTTAAGGC+TGG + chr1_4:14200364-14200383 None:intergenic 30.0%
!! TTAAAGCAAAGTGCTTTTCT+TGG - chr1_4:14200617-14200636 Msa0151840:intron 30.0%
TGGCTAGATTTGGAAAATTT+AGG - chr1_4:14200637-14200656 Msa0151840:intron 30.0%
ATATGCTATAACAGTGTACT+AGG - chr1_4:14200725-14200744 Msa0151840:intron 30.0%
AGAACAGTCAAAAAATTGCT+CGG + chr1_4:14200977-14200996 None:intergenic 30.0%
GGAAAAAATATCACAAGACA+AGG + chr1_4:14201629-14201648 None:intergenic 30.0%
!! AGAAATACTGACTTTGTTAG+CGG + chr1_4:14201711-14201730 None:intergenic 30.0%
CTGTTTGAAAAACTAATCAC+TGG - chr1_4:14201926-14201945 Msa0151840:intron 30.0%
ATGCTTGAAATTTGTAGCTA+CGG + chr1_4:14202140-14202159 None:intergenic 30.0%
AAAATATCGAGCTTGAAATC+CGG + chr1_4:14202201-14202220 None:intergenic 30.0%
CTCAACTATTTGTAAATGGA+TGG - chr1_4:14202363-14202382 Msa0151840:intron 30.0%
CCCGGTTTAGAAAATAAATT+TGG + chr1_4:14202446-14202465 None:intergenic 30.0%
CAAAATTGTTCCTTGTACTT+AGG + chr1_4:14202537-14202556 None:intergenic 30.0%
TTGAAATGACATCATGTTTG+AGG - chr1_4:14202627-14202646 Msa0151840:five_prime_UTR 30.0%
AACAAGTAACAGCTAAGACA+TGG - chr1_4:14199873-14199892 Msa0151840:three_prime_UTR 35.0%
TCTCATCAATTCAGATGATC+AGG - chr1_4:14199936-14199955 Msa0151840:three_prime_UTR 35.0%
GATTTCTGTGGAACAATTCT+TGG + chr1_4:14200513-14200532 None:intergenic 35.0%
CAATCTAGATTCGATTTCTG+TGG + chr1_4:14200525-14200544 None:intergenic 35.0%
! TAATTTGTATGACTACGACG+AGG - chr1_4:14200782-14200801 Msa0151840:intron 35.0%
!!! ACTGTTCTTTTGGGGTTTTT+AGG - chr1_4:14200989-14201008 Msa0151840:intron 35.0%
!!! CTGTTCTTTTGGGGTTTTTA+GGG - chr1_4:14200990-14201009 Msa0151840:intron 35.0%
!!! TTTTAGGGAGGTATTGAAGA+TGG - chr1_4:14201005-14201024 Msa0151840:intron 35.0%
TCTCTAATAGCAGCAGATTT+GGG + chr1_4:14201038-14201057 None:intergenic 35.0%
TTCTCTAATAGCAGCAGATT+TGG + chr1_4:14201039-14201058 None:intergenic 35.0%
ATCAATACTCCTTAGAGCAT+CGG + chr1_4:14201180-14201199 None:intergenic 35.0%
ATTTCTCTGTGATAACTCTG+TGG - chr1_4:14201301-14201320 Msa0151840:CDS 35.0%
!! GTGCATTACAGGTTGATAAA+TGG - chr1_4:14201356-14201375 Msa0151840:intron 35.0%
TGCATCAATGAAAGCGATAT+AGG - chr1_4:14201570-14201589 Msa0151840:intron 35.0%
ATTTCCACTCTGCAAATTCT+GGG + chr1_4:14201834-14201853 None:intergenic 35.0%
AGAATTTGCAGAGTGGAAAT+GGG - chr1_4:14201834-14201853 Msa0151840:intron 35.0%
GTTACAACTTCTTCATAGGT+AGG + chr1_4:14201988-14202007 None:intergenic 35.0%
GGTTGTTACAACTTCTTCAT+AGG + chr1_4:14201992-14202011 None:intergenic 35.0%
GTTGCATTTCCTTGAAAGTA+AGG + chr1_4:14202021-14202040 None:intergenic 35.0%
CGAAATGTAAATCGACAAAG+TGG - chr1_4:14202046-14202065 Msa0151840:intron 35.0%
!! ATTGTCTGATTTTGACCCAA+AGG + chr1_4:14202178-14202197 None:intergenic 35.0%
! CTCAGTAAGTGAAATGGATT+TGG + chr1_4:14202330-14202349 None:intergenic 35.0%
CAAATCCATTTCACTTACTG+AGG - chr1_4:14202328-14202347 Msa0151840:intron 35.0%
GGTAGTTAGAACTTTCCATT+AGG - chr1_4:14202384-14202403 Msa0151840:intron 35.0%
TACAAGAAGCAAGTACCTAA+TGG + chr1_4:14202402-14202421 None:intergenic 35.0%
TGACTTGATACCTAAGTACA+AGG - chr1_4:14202524-14202543 Msa0151840:five_prime_UTR 35.0%
GAACATGTTAGAATGGTTGA+TGG + chr1_4:14202606-14202625 None:intergenic 35.0%
CAACACAAACACAACACAAG+TGG + chr1_4:14199750-14199769 None:intergenic 40.0%
GTGTTGTGTTAGAGATGATG+AGG + chr1_4:14199795-14199814 None:intergenic 40.0%
TCCATCAAAGGACAATCGTT+CGG + chr1_4:14200550-14200569 None:intergenic 40.0%
TAACCGCAAACCAAGTGATT+GGG + chr1_4:14200591-14200610 None:intergenic 40.0%
ATAACCGCAAACCAAGTGAT+TGG + chr1_4:14200592-14200611 None:intergenic 40.0%
! GTGCTTTTCTTGGCTAGATT+TGG - chr1_4:14200627-14200646 Msa0151840:intron 40.0%
CAGTGTACTAGGAATTAGAG+AGG - chr1_4:14200736-14200755 Msa0151840:intron 40.0%
ACATAATATGAGGTCACCTG+AGG + chr1_4:14200887-14200906 None:intergenic 40.0%
!!! TTCTTTTGGGGTTTTTAGGG+AGG - chr1_4:14200993-14201012 Msa0151840:intron 40.0%
AGAGAACTTCGAGAAGAAAC+TGG - chr1_4:14201053-14201072 Msa0151840:intron 40.0%
TGACAGTGTTTGTGCATTAC+AGG - chr1_4:14201345-14201364 Msa0151840:intron 40.0%
AGGCTAAAGTCAATCGTCTT+TGG - chr1_4:14201407-14201426 Msa0151840:intron 40.0%
GGCTAAAGTCAATCGTCTTT+GGG - chr1_4:14201408-14201427 Msa0151840:intron 40.0%
GCTAAAGTCAATCGTCTTTG+GGG - chr1_4:14201409-14201428 Msa0151840:intron 40.0%
CATTTCCACTCTGCAAATTC+TGG + chr1_4:14201835-14201854 None:intergenic 40.0%
CAGAATTTGCAGAGTGGAAA+TGG - chr1_4:14201833-14201852 Msa0151840:intron 40.0%
CCTGCTCAATAACTTCTTCA+GGG + chr1_4:14201864-14201883 None:intergenic 40.0%
ACCTGCTCAATAACTTCTTC+AGG + chr1_4:14201865-14201884 None:intergenic 40.0%
CTGGGCTAATAATCGATTAC+AGG - chr1_4:14201945-14201964 Msa0151840:intron 40.0%
GGCTAATAATCGATTACAGG+CGG - chr1_4:14201948-14201967 Msa0151840:intron 40.0%
TAATAATCGATTACAGGCGG+TGG - chr1_4:14201951-14201970 Msa0151840:intron 40.0%
ACCTTCAAGCCTTACTTTCA+AGG - chr1_4:14202009-14202028 Msa0151840:intron 40.0%
TCCTTGAAAGTAAGGCTTGA+AGG + chr1_4:14202013-14202032 None:intergenic 40.0%
TGGGTCAAAATCAGACAATC+CGG - chr1_4:14202179-14202198 Msa0151840:intron 40.0%
TCCATTTCACTTACTGAGGT+TGG - chr1_4:14202332-14202351 Msa0151840:intron 40.0%
TCCAACCTCAGTAAGTGAAA+TGG + chr1_4:14202336-14202355 None:intergenic 40.0%
GTATAGATCTGTTGATGTCG+CGG - chr1_4:14202490-14202509 Msa0151840:five_prime_UTR 40.0%
GAGGTGTGAATGATGAGTAC+TGG + chr1_4:14199776-14199795 None:intergenic 45.0%
GACATGGAGAATCTTCCACA+AGG - chr1_4:14199889-14199908 Msa0151840:three_prime_UTR 45.0%
ATGAGACAGACACCAACGTT+AGG + chr1_4:14199922-14199941 None:intergenic 45.0%
! TTGTTAACCCTCTGGTTCAG+TGG - chr1_4:14200444-14200463 Msa0151840:intron 45.0%
GGAAGGACCTGATAATTCGT+CGG - chr1_4:14200465-14200484 Msa0151840:intron 45.0%
TTCACGACCGACGAATTATC+AGG + chr1_4:14200475-14200494 None:intergenic 45.0%
CCATCAAAGGACAATCGTTC+GGG + chr1_4:14200549-14200568 None:intergenic 45.0%
ATCACTTGAGCCCAATCACT+TGG - chr1_4:14200578-14200597 Msa0151840:intron 45.0%
TTGTATGACTACGACGAGGA+AGG - chr1_4:14200786-14200805 Msa0151840:intron 45.0%
TGTATGACTACGACGAGGAA+GGG - chr1_4:14200787-14200806 Msa0151840:intron 45.0%
GCTTCCAGATTGAATGTTCC+AGG - chr1_4:14200848-14200867 Msa0151840:intron 45.0%
TGCTCCTGGAACATTCAATC+TGG + chr1_4:14200855-14200874 None:intergenic 45.0%
GATTGAATGTTCCAGGAGCA+TGG - chr1_4:14200855-14200874 Msa0151840:intron 45.0%
! TTGACTTTAGCCTTCACAGC+AGG + chr1_4:14201400-14201419 None:intergenic 45.0%
! AAAGTCAATCGTCTTTGGGG+AGG - chr1_4:14201412-14201431 Msa0151840:intron 45.0%
CATTTACTCCATGCACACTC+AGG + chr1_4:14201599-14201618 None:intergenic 45.0%
CTGAGTGTGCATGGAGTAAA+TGG - chr1_4:14201597-14201616 Msa0151840:intron 45.0%
GGTGAAATCAACTTAGCCAC+TGG - chr1_4:14201799-14201818 Msa0151840:intron 45.0%
CCCTGAAGAAGTTATTGAGC+AGG - chr1_4:14201861-14201880 Msa0151840:intron 45.0%
AGACAATGCAGAAAGCAAGC+AGG + chr1_4:14202249-14202268 None:intergenic 45.0%
!!! TTTTTTATTAATGAAAATTT+AGG - chr1_4:14200018-14200037 Msa0151840:three_prime_UTR 5.0%
ACGTTAGGACGGTAACCTTG+TGG + chr1_4:14199907-14199926 None:intergenic 50.0%
CAAGGTTACCGTCCTAACGT+TGG - chr1_4:14199907-14199926 Msa0151840:three_prime_UTR 50.0%
GACAGACACCAACGTTAGGA+CGG + chr1_4:14199918-14199937 None:intergenic 50.0%
! TAACCCTCTGGTTCAGTGGA+AGG - chr1_4:14200448-14200467 Msa0151840:intron 50.0%
CCCGAACGATTGTCCTTTGA+TGG - chr1_4:14200546-14200565 Msa0151840:CDS 50.0%
! GAGCCCAATCACTTGGTTTG+CGG - chr1_4:14200585-14200604 Msa0151840:intron 50.0%
ACGACGAGGAAGGGAAATCA+TGG - chr1_4:14200796-14200815 Msa0151840:intron 50.0%
GTCTGCTGAGATAATCGCTG+AGG - chr1_4:14201082-14201101 Msa0151840:intron 50.0%
TACGTCTAGCCGATGCTCTA+AGG - chr1_4:14201168-14201187 Msa0151840:intron 50.0%
! CTTTAGCCTTCACAGCAGGA+GGG + chr1_4:14201396-14201415 None:intergenic 50.0%
! ACTTTAGCCTTCACAGCAGG+AGG + chr1_4:14201397-14201416 None:intergenic 50.0%
! ATCGTCTTTGGGGAGGAGAA+TGG - chr1_4:14201419-14201438 Msa0151840:intron 50.0%
ATAGGAAGCCTGAGTGTGCA+TGG - chr1_4:14201588-14201607 Msa0151840:intron 50.0%
CAGACCCAGAATTTGCAGAG+TGG - chr1_4:14201827-14201846 Msa0151840:intron 50.0%
TTCGCGTACATCTGTGCCTT+TGG - chr1_4:14202159-14202178 Msa0151840:intron 50.0%
TCGCGTACATCTGTGCCTTT+GGG - chr1_4:14202160-14202179 Msa0151840:intron 50.0%
TTCGCACTGTCAACACGTGT+TGG + chr1_4:14202281-14202300 None:intergenic 50.0%
! GTGTTGACAGTGCGAACGTA+TGG - chr1_4:14202285-14202304 Msa0151840:intron 50.0%
! ATTTTCTAAACCGGGCTGCG+AGG - chr1_4:14202451-14202470 Msa0151840:CDS 50.0%
GGTCCTTCCACTGAACCAGA+GGG + chr1_4:14200454-14200473 None:intergenic 55.0%
AGGTCCTTCCACTGAACCAG+AGG + chr1_4:14200455-14200474 None:intergenic 55.0%
! TGAGGCATTTGCCATGCTCC+TGG + chr1_4:14200869-14200888 None:intergenic 55.0%
! AGGAGCATGGCAAATGCCTC+AGG - chr1_4:14200868-14200887 Msa0151840:intron 55.0%
TGACTTCCCTCCTGCTGTGA+AGG - chr1_4:14201387-14201406 Msa0151840:intron 55.0%
! CTTTGGGGAGGAGAATGGCA+TGG - chr1_4:14201424-14201443 Msa0151840:intron 55.0%
GGCATGGACAGGCACAGAAA+TGG - chr1_4:14201440-14201459 Msa0151840:intron 55.0%
TTCTGGGTCTGCTTCACCAG+TGG + chr1_4:14201818-14201837 None:intergenic 55.0%
TCACAATCATCCTCGCAGCC+CGG + chr1_4:14202464-14202483 None:intergenic 55.0%
GGGAGGAGAATGGCATGGAC+AGG - chr1_4:14201429-14201448 Msa0151840:intron 60.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 14199708 14202656 14199708 ID=Msa0151840;Name=Msa0151840
chr1_4 mRNA 14199708 14202656 14199708 ID=Msa0151840-mRNA-1;Parent=Msa0151840;Name=Msa0151840-mRNA-1;_AED=0.14;_eAED=0.14;_QI=184|1|1|1|1|1|6|585|173
chr1_4 exon 14199708 14200397 14199708 ID=Msa0151840-mRNA-1:exon:5623;Parent=Msa0151840-mRNA-1
chr1_4 exon 14200482 14200593 14200482 ID=Msa0151840-mRNA-1:exon:5622;Parent=Msa0151840-mRNA-1
chr1_4 exon 14200903 14200997 14200903 ID=Msa0151840-mRNA-1:exon:5621;Parent=Msa0151840-mRNA-1
chr1_4 exon 14201261 14201353 14201261 ID=Msa0151840-mRNA-1:exon:5620;Parent=Msa0151840-mRNA-1
chr1_4 exon 14201475 14201525 14201475 ID=Msa0151840-mRNA-1:exon:5619;Parent=Msa0151840-mRNA-1
chr1_4 exon 14202407 14202656 14202407 ID=Msa0151840-mRNA-1:exon:5618;Parent=Msa0151840-mRNA-1
chr1_4 five_prime_UTR 14202473 14202656 14202473 ID=Msa0151840-mRNA-1:five_prime_utr;Parent=Msa0151840-mRNA-1
chr1_4 CDS 14202407 14202472 14202407 ID=Msa0151840-mRNA-1:cds;Parent=Msa0151840-mRNA-1
chr1_4 CDS 14201475 14201525 14201475 ID=Msa0151840-mRNA-1:cds;Parent=Msa0151840-mRNA-1
chr1_4 CDS 14201261 14201353 14201261 ID=Msa0151840-mRNA-1:cds;Parent=Msa0151840-mRNA-1
chr1_4 CDS 14200903 14200997 14200903 ID=Msa0151840-mRNA-1:cds;Parent=Msa0151840-mRNA-1
chr1_4 CDS 14200482 14200593 14200482 ID=Msa0151840-mRNA-1:cds;Parent=Msa0151840-mRNA-1
chr1_4 CDS 14200293 14200397 14200293 ID=Msa0151840-mRNA-1:cds;Parent=Msa0151840-mRNA-1
chr1_4 three_prime_UTR 14199708 14200292 14199708 ID=Msa0151840-mRNA-1:three_prime_utr;Parent=Msa0151840-mRNA-1
Gene Sequence

>Msa0151840

ATGGAGAATCTTCCACAAGGTTACCGTCCTAACGTTGGTGTCTGTCTCATCAATTCAGATGATCAGATATTTGTGGCTTCCAGATTGAATGTTCCAGGAGCATGGCAAATGCCTCAGGGAGGTATTGAAGATGGCGAAGAGCCCAAATCTGCTGCTATTAGAGAACTTCGAGAAGAAACTGGAATCGTGTCTGCTGAGATAATCGCTGAGGTTGATAAATGGTTGACATATGACTTCCCTCCTGCTGTGAAGGCTAAAGTCAATCGTCTTTGGGGAGGAGAATGGCATGGACAGGCACAGAAATGGTTTCTCATGAAGTTAAAAAAAGATGACGGTGAAATCAACTTAGCCACTGGTGAAGCAGACCCAGAATTTGCAGAGTGGAAATGGGCAAGCCCTGAAGAAGTTATTGAGCAGGCGGTGGACTACAAGAGACCTACCTATGAAGAAGTTGTAACAACCTTCAAGCCTTACTTTCAAGGAAATGCAACATCTGCGAAATGTAAATCGACAAAGTGGTGA
Protein sequence

>Msa0151840

MENLPQGYRPNVGVCLINSDDQIFVASRLNVPGAWQMPQGGIEDGEEPKSAAIRELREETGIVSAEIIAEVDKWLTYDFPPAVKAKVNRLWGGEWHGQAQKWFLMKLKKDDGEINLATGEADPEFAEWKWASPEEVIEQAVDYKRPTYEEVVTTFKPYFQGNATSAKCKSTKW*