AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0178740

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0178740 MtrunA17_Chr1g0207351 70.655 351 94 4 22 363 16 366 3.35e-165 466
Msa0178740 MtrunA17_Chr3g0093911 68.091 351 106 4 18 363 1 350 5.53e-159 449
Msa0178740 MtrunA17_Chr7g0252101 66.761 355 109 6 15 363 81 432 8.26e-153 437
Msa0178740 MtrunA17_Chr4g0075971 60.563 355 135 4 13 363 249 602 6.05e-130 385
Msa0178740 MtrunA17_Chr3g0077801 57.593 349 142 3 21 363 4 352 9.13e-129 373
Msa0178740 MtrunA17_Chr3g0077901 57.303 356 142 6 15 362 18 371 3.92e-127 369
Msa0178740 MtrunA17_Chr3g0077851 58.160 337 131 6 34 362 1 335 2.82e-122 356
Msa0178740 MtrunA17_Chr7g0243331 54.830 352 149 5 20 363 1 350 5.57e-119 348
Msa0178740 MtrunA17_Chr6g0488101 53.571 364 150 6 15 363 96 455 3.14e-118 350
Msa0178740 MtrunA17_Chr6g0488271 53.297 364 151 6 15 363 56 415 5.97e-118 348
Msa0178740 MtrunA17_Chr1g0209051 66.667 270 84 3 99 362 1 270 1.43e-115 336
Msa0178740 MtrunA17_Chr3g0112311 54.749 358 145 5 18 361 1 355 2.52e-114 336
Msa0178740 MtrunA17_Chr6g0452641 52.336 321 129 7 61 363 26 340 4.97e-88 268
Msa0178740 MtrunA17_Chr3g0122991 47.170 318 109 5 23 329 5 274 2.78e-82 251
Msa0178740 MtrunA17_Chr1g0202841 66.480 179 54 3 191 363 1 179 1.78e-73 226
Msa0178740 MtrunA17_Chr3g0123471 51.923 260 113 6 103 355 5 259 1.08e-71 224
Msa0178740 MtrunA17_Chr1g0206951 84.211 114 17 1 20 132 1 114 4.10e-64 200
Msa0178740 MtrunA17_Chr7g0243391 49.765 213 103 2 15 225 15 225 1.01e-54 180
Msa0178740 MtrunA17_Chr1g0187941 62.044 137 48 3 191 323 1 137 2.01e-46 155
Msa0178740 MtrunA17_Chr1g0196231 60.544 147 53 3 15 160 224 366 6.59e-46 160
Msa0178740 MtrunA17_Chr6g0486591 48.428 159 69 3 218 363 1 159 3.99e-39 136
Msa0178740 MtrunA17_Chr4g0075861 64.286 126 44 1 13 138 249 373 5.33e-38 140
Msa0178740 MtrunA17_Chr1g0209191 67.347 98 25 1 272 362 1 98 7.40e-36 126
Msa0178740 MtrunA17_Chr1g0196241 74.390 82 20 1 263 343 3 84 5.41e-33 118
Msa0178740 MtrunA17_Chr6g0459061 27.326 344 235 8 26 360 18 355 1.58e-26 108
Msa0178740 MtrunA17_Chr7g0238781 38.806 134 78 3 50 181 27 158 2.01e-18 81.6
Msa0178740 MtrunA17_Chr1g0209061 67.347 49 16 0 9 57 59 107 2.36e-17 77.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 72 sgRNAs with CRISPR-Local

Find 196 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACCCCTATTTCTAAAGTTAA+AGG 0.132036 1_4:-66144103 Msa0178740:CDS
GAACCTTTAACTTTAGAAAT+AGG 0.154789 1_4:+66144100 None:intergenic
AACCTTTAACTTTAGAAATA+GGG 0.214014 1_4:+66144101 None:intergenic
AATTTACTCTAGAAATTTCT+TGG 0.228388 1_4:-66144344 Msa0178740:CDS
TTTGAAGAAGTAAAGGTTCT+TGG 0.278248 1_4:+66144317 None:intergenic
CTTTCATATCCCCACCTGTT+GGG 0.307053 1_4:+66143626 None:intergenic
TAATCAAACTAAAGGAAAAT+AGG 0.313420 1_4:+66143386 None:intergenic
AGACGTGCCTTGCTTGCTTT+TGG 0.336224 1_4:-66144067 Msa0178740:CDS
ATCAATCTCGAAATTTAGAT+TGG 0.339092 1_4:-66143988 Msa0178740:CDS
TTCCAGTTTGACGTAATTCA+TGG 0.341057 1_4:+66143709 None:intergenic
TTCAAGTTGTAGTGTGTATT+CGG 0.358079 1_4:-66143573 Msa0178740:CDS
GTGTAAAAGCAAAAGCTTCC+AGG 0.365898 1_4:+66143818 None:intergenic
AAAAGTTTATTGCTCGAAGA+TGG 0.381429 1_4:-66144245 Msa0178740:CDS
TTGAAGAAGTAAAGGTTCTT+GGG 0.388728 1_4:+66144318 None:intergenic
AGAGAGCCGTGAACTTCCTT+TGG 0.390301 1_4:-66144455 Msa0178740:CDS
AACCATGAATTACGTCAAAC+TGG 0.393600 1_4:-66143711 Msa0178740:CDS
GTCGTCAAAATCTAGTGTTT+TGG 0.396017 1_4:+66144414 None:intergenic
CTCCGAGGAATTTGTCTTGG+TGG 0.398241 1_4:-66144038 Msa0178740:CDS
TTACGTCAAACTGGAAACTT+TGG 0.400584 1_4:-66143702 Msa0178740:CDS
TTTATGATACAAAGGAAAAC+AGG 0.417030 1_4:-66144491 Msa0178740:intron
TTGGTCGGGTATGCAAATAA+AGG 0.421092 1_4:+66144267 None:intergenic
CAAGAAATTTGAAGAAGTAA+AGG 0.425776 1_4:+66144310 None:intergenic
AAAACACCAATCAGACTTGA+AGG 0.428047 1_4:+66143493 None:intergenic
TTTCTGGTGTGTGCAAGAAT+TGG 0.430304 1_4:-66144381 Msa0178740:CDS
TCAAATTTCTTGTCAACCTA+CGG 0.433760 1_4:-66144299 Msa0178740:CDS
CAAGTTCCTTCAAGTCTGAT+TGG 0.438389 1_4:-66143499 Msa0178740:CDS
ATGCATTGAGCAACCCCAAC+AGG 0.449427 1_4:-66143640 Msa0178740:CDS
TCGAGCCCTAATCAAACTAA+AGG 0.449702 1_4:+66143378 None:intergenic
ATTGTACACCTTGTTACCTA+TGG 0.452529 1_4:-66143421 Msa0178740:three_prime_UTR
CTGGTGTGTGCAAGAATTGG+AGG 0.458932 1_4:-66144378 Msa0178740:CDS
TACGTCAAACTGGAAACTTT+GGG 0.462690 1_4:-66143701 Msa0178740:CDS
TTGGGTTGCTTATTCGAGAA+TGG 0.468267 1_4:-66143969 Msa0178740:CDS
TCAATCTCGAAATTTAGATT+GGG 0.470050 1_4:-66143987 Msa0178740:CDS
AAAGTTTATTGCTCGAAGAT+GGG 0.471695 1_4:-66144244 Msa0178740:CDS
ACTAGGTTGGTTAATTGTGA+TGG 0.475319 1_4:-66143797 Msa0178740:CDS
CAAATTTCTTGTCAACCTAC+GGG 0.493687 1_4:-66144298 Msa0178740:CDS
TGAGAAAATAATACATCGAT+TGG 0.494657 1_4:-66143313 Msa0178740:three_prime_UTR
CTAAAACTGAAGAGTACTCC+TGG 0.499046 1_4:-66143836 Msa0178740:CDS
ATTACACTTTCAACTCCTCA+TGG 0.500145 1_4:-66143524 Msa0178740:CDS
ACCTTTAACTTTAGAAATAG+GGG 0.506391 1_4:+66144102 None:intergenic
GCTTTCATATCCCCACCTGT+TGG 0.512470 1_4:+66143625 None:intergenic
TTTCATATCCCCACCTGTTG+GGG 0.516850 1_4:+66143627 None:intergenic
CAAAGGAAAACAGGTGAAGA+TGG 0.519464 1_4:-66144482 Msa0178740:intron
AGAGGTAATTGTTGACTTCG+TGG 0.525520 1_4:-66143939 Msa0178740:CDS
AAGCTCCGAGGAATTTGTCT+TGG 0.528013 1_4:-66144041 Msa0178740:CDS
TCACTGACAAGGCCAACATA+GGG 0.528379 1_4:-66143892 Msa0178740:CDS
CTAGGTTGGTTAATTGTGAT+GGG 0.532467 1_4:-66143796 Msa0178740:CDS
AGTTGTAGTGTGTATTCGGT+TGG 0.533748 1_4:-66143569 Msa0178740:CDS
ACGAAGTCAACAATTACCTC+TGG 0.541358 1_4:+66143941 None:intergenic
CAAGCTGAGCACCCCTATGT+TGG 0.542473 1_4:+66143880 None:intergenic
GTCACTGACAAGGCCAACAT+AGG 0.554026 1_4:-66143893 Msa0178740:CDS
GCACATCCAAAGGAAGTTCA+CGG 0.557808 1_4:+66144449 None:intergenic
AAGTTTATTGCTCGAAGATG+GGG 0.576744 1_4:-66144243 Msa0178740:CDS
TCCGCTAGAAATCGTAACAT+AGG 0.576857 1_4:+66144201 None:intergenic
TGGTGTGTGCAAGAATTGGA+GGG 0.582905 1_4:-66144377 Msa0178740:CDS
AACCACCAAGACAAATTCCT+CGG 0.591881 1_4:+66144036 None:intergenic
TAGGGCTCGAAAACTTGTAG+TGG 0.591929 1_4:-66143365 Msa0178740:three_prime_UTR
CATTGAGCAACCCCAACAGG+TGG 0.595743 1_4:-66143637 Msa0178740:CDS
ACACACCAGAAAATTGAAAG+TGG 0.598319 1_4:+66144392 None:intergenic
GCCTATGTTACGATTTCTAG+CGG 0.604035 1_4:-66144202 Msa0178740:CDS
ATGATATCAAGCACATCCAA+AGG 0.608076 1_4:+66144439 None:intergenic
TCAAACTAAAGGAAAATAGG+TGG 0.608701 1_4:+66143389 None:intergenic
TAGGTGGAAACACAGACCAT+AGG 0.616833 1_4:+66143405 None:intergenic
ATTGAGCAACCCCAACAGGT+GGG 0.636644 1_4:-66143636 Msa0178740:CDS
TTCGAGAATGGAAGATCCAG+AGG 0.642137 1_4:-66143957 Msa0178740:CDS
AACACAGACCATAGGTAACA+AGG 0.645089 1_4:+66143413 None:intergenic
CACTGACAAGGCCAACATAG+GGG 0.655325 1_4:-66143891 Msa0178740:CDS
TTGAGCAACCCCAACAGGTG+GGG 0.661540 1_4:-66143635 Msa0178740:CDS
ATAAAGGAAAATGATCCCGT+AGG 0.662758 1_4:+66144283 None:intergenic
AATATATGTTGTCACTGACA+AGG 0.674972 1_4:-66143903 Msa0178740:CDS
TTGAAGGAACTTGTTCCATG+AGG 0.687755 1_4:+66143509 None:intergenic
AAATCGTAACATAGGCAGAG+CGG 0.737024 1_4:+66144209 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATCATAAAATGAAAATATT+AGG + chr1_4:66143402-66143421 None:intergenic 10.0%
!! TATCATAAAATGAAAATATT+AGG + chr1_4:66143402-66143421 None:intergenic 10.0%
!!! ATTTTCATTTTATGATACAA+AGG - chr1_4:66143405-66143424 Msa0178740:three_prime_UTR 15.0%
!!! ATTTTCATTTTATGATACAA+AGG - chr1_4:66143405-66143424 Msa0178740:three_prime_UTR 15.0%
!!! AATTTACTCTAGAAATTTCT+TGG - chr1_4:66143560-66143579 Msa0178740:CDS 20.0%
!! TCCATTTACAAGAATAAAAA+AGG - chr1_4:66143770-66143789 Msa0178740:CDS 20.0%
!!! ACCTTTTTTATTCTTGTAAA+TGG + chr1_4:66143774-66143793 None:intergenic 20.0%
!! ATTTACAAGAATAAAAAAGG+TGG - chr1_4:66143773-66143792 Msa0178740:CDS 20.0%
!!! AACCTTTAACTTTAGAAATA+GGG + chr1_4:66143806-66143825 None:intergenic 20.0%
!!! GTACACATTACATATTTTAT+TGG + chr1_4:66144159-66144178 None:intergenic 20.0%
!! TAATCAAACTAAAGGAAAAT+AGG + chr1_4:66144521-66144540 None:intergenic 20.0%
!!! TATTTTCCTTTAGTTTGATT+AGG - chr1_4:66144520-66144539 Msa0178740:intron 20.0%
!!! ATTTTCCTTTAGTTTGATTA+GGG - chr1_4:66144521-66144540 Msa0178740:intron 20.0%
!!! AATTTACTCTAGAAATTTCT+TGG - chr1_4:66143560-66143579 Msa0178740:CDS 20.0%
!! TCCATTTACAAGAATAAAAA+AGG - chr1_4:66143770-66143789 Msa0178740:CDS 20.0%
!!! ACCTTTTTTATTCTTGTAAA+TGG + chr1_4:66143774-66143793 None:intergenic 20.0%
!! ATTTACAAGAATAAAAAAGG+TGG - chr1_4:66143773-66143792 Msa0178740:CDS 20.0%
!!! AACCTTTAACTTTAGAAATA+GGG + chr1_4:66143806-66143825 None:intergenic 20.0%
!!! GTACACATTACATATTTTAT+TGG + chr1_4:66144159-66144178 None:intergenic 20.0%
!! TAATCAAACTAAAGGAAAAT+AGG + chr1_4:66144521-66144540 None:intergenic 20.0%
!!! TATTTTCCTTTAGTTTGATT+AGG - chr1_4:66144520-66144539 Msa0178740:intron 20.0%
!!! ATTTTCCTTTAGTTTGATTA+GGG - chr1_4:66144521-66144540 Msa0178740:intron 20.0%
!!! GTTGATATGGATTTTTGTTT+GGG - chr1_4:66143290-66143309 Msa0178740:three_prime_UTR 25.0%
!!! TTGATATGGATTTTTGTTTG+GGG - chr1_4:66143291-66143310 Msa0178740:three_prime_UTR 25.0%
! TTTATGATACAAAGGAAAAC+AGG - chr1_4:66143413-66143432 Msa0178740:three_prime_UTR 25.0%
! CAAGAAATTTGAAGAAGTAA+AGG + chr1_4:66143597-66143616 None:intergenic 25.0%
!! ACCTTTAACTTTAGAAATAG+GGG + chr1_4:66143805-66143824 None:intergenic 25.0%
!! GAACCTTTAACTTTAGAAAT+AGG + chr1_4:66143807-66143826 None:intergenic 25.0%
! ATCAATCTCGAAATTTAGAT+TGG - chr1_4:66143916-66143935 Msa0178740:CDS 25.0%
! TCAATCTCGAAATTTAGATT+GGG - chr1_4:66143917-66143936 Msa0178740:CDS 25.0%
!! TTTTAGACATCAACATTTTG+AGG - chr1_4:66144430-66144449 Msa0178740:CDS 25.0%
! TGAGAAAATAATACATCGAT+TGG - chr1_4:66144591-66144610 Msa0178740:intron 25.0%
!!! GTTGATATGGATTTTTGTTT+GGG - chr1_4:66143290-66143309 Msa0178740:three_prime_UTR 25.0%
!!! TTGATATGGATTTTTGTTTG+GGG - chr1_4:66143291-66143310 Msa0178740:three_prime_UTR 25.0%
! TTTATGATACAAAGGAAAAC+AGG - chr1_4:66143413-66143432 Msa0178740:three_prime_UTR 25.0%
! CAAGAAATTTGAAGAAGTAA+AGG + chr1_4:66143597-66143616 None:intergenic 25.0%
!! ACCTTTAACTTTAGAAATAG+GGG + chr1_4:66143805-66143824 None:intergenic 25.0%
!! GAACCTTTAACTTTAGAAAT+AGG + chr1_4:66143807-66143826 None:intergenic 25.0%
! ATCAATCTCGAAATTTAGAT+TGG - chr1_4:66143916-66143935 Msa0178740:CDS 25.0%
! TCAATCTCGAAATTTAGATT+GGG - chr1_4:66143917-66143936 Msa0178740:CDS 25.0%
!! TTTTAGACATCAACATTTTG+AGG - chr1_4:66144430-66144449 Msa0178740:CDS 25.0%
! TGAGAAAATAATACATCGAT+TGG - chr1_4:66144591-66144610 Msa0178740:intron 25.0%
! CGTTGATATGGATTTTTGTT+TGG - chr1_4:66143289-66143308 Msa0178740:three_prime_UTR 30.0%
!! ATTTTTGTTTGGGGAAAATG+CGG - chr1_4:66143300-66143319 Msa0178740:three_prime_UTR 30.0%
TTGAAGAAGTAAAGGTTCTT+GGG + chr1_4:66143589-66143608 None:intergenic 30.0%
TTTGAAGAAGTAAAGGTTCT+TGG + chr1_4:66143590-66143609 None:intergenic 30.0%
TCAAATTTCTTGTCAACCTA+CGG - chr1_4:66143605-66143624 Msa0178740:CDS 30.0%
! TCTTCGAGCAATAAACTTTT+TGG + chr1_4:66143659-66143678 None:intergenic 30.0%
AAAAGTTTATTGCTCGAAGA+TGG - chr1_4:66143659-66143678 Msa0178740:CDS 30.0%
AAAGTTTATTGCTCGAAGAT+GGG - chr1_4:66143660-66143679 Msa0178740:CDS 30.0%
!! TTCTAGCGGATATTTTATCA+TGG - chr1_4:66143716-66143735 Msa0178740:CDS 30.0%
!! ACCCCTATTTCTAAAGTTAA+AGG - chr1_4:66143801-66143820 Msa0178740:CDS 30.0%
AATATATGTTGTCACTGACA+AGG - chr1_4:66144001-66144020 Msa0178740:CDS 30.0%
!! TTCAAGTTGTAGTGTGTATT+CGG - chr1_4:66144331-66144350 Msa0178740:CDS 30.0%
TCAAACTAAAGGAAAATAGG+TGG + chr1_4:66144518-66144537 None:intergenic 30.0%
! CGTTGATATGGATTTTTGTT+TGG - chr1_4:66143289-66143308 Msa0178740:three_prime_UTR 30.0%
!! ATTTTTGTTTGGGGAAAATG+CGG - chr1_4:66143300-66143319 Msa0178740:three_prime_UTR 30.0%
TTGAAGAAGTAAAGGTTCTT+GGG + chr1_4:66143589-66143608 None:intergenic 30.0%
TTTGAAGAAGTAAAGGTTCT+TGG + chr1_4:66143590-66143609 None:intergenic 30.0%
TCAAATTTCTTGTCAACCTA+CGG - chr1_4:66143605-66143624 Msa0178740:CDS 30.0%
! TCTTCGAGCAATAAACTTTT+TGG + chr1_4:66143659-66143678 None:intergenic 30.0%
AAAAGTTTATTGCTCGAAGA+TGG - chr1_4:66143659-66143678 Msa0178740:CDS 30.0%
AAAGTTTATTGCTCGAAGAT+GGG - chr1_4:66143660-66143679 Msa0178740:CDS 30.0%
!! TTCTAGCGGATATTTTATCA+TGG - chr1_4:66143716-66143735 Msa0178740:CDS 30.0%
!! ACCCCTATTTCTAAAGTTAA+AGG - chr1_4:66143801-66143820 Msa0178740:CDS 30.0%
AATATATGTTGTCACTGACA+AGG - chr1_4:66144001-66144020 Msa0178740:CDS 30.0%
!! TTCAAGTTGTAGTGTGTATT+CGG - chr1_4:66144331-66144350 Msa0178740:CDS 30.0%
TCAAACTAAAGGAAAATAGG+TGG + chr1_4:66144518-66144537 None:intergenic 30.0%
ATGATATCAAGCACATCCAA+AGG + chr1_4:66143468-66143487 None:intergenic 35.0%
! GTCGTCAAAATCTAGTGTTT+TGG + chr1_4:66143493-66143512 None:intergenic 35.0%
ACACACCAGAAAATTGAAAG+TGG + chr1_4:66143515-66143534 None:intergenic 35.0%
CAAATTTCTTGTCAACCTAC+GGG - chr1_4:66143606-66143625 Msa0178740:CDS 35.0%
ATAAAGGAAAATGATCCCGT+AGG + chr1_4:66143624-66143643 None:intergenic 35.0%
!! GAGCAATAAACTTTTTGGTC+GGG + chr1_4:66143654-66143673 None:intergenic 35.0%
!! CGAGCAATAAACTTTTTGGT+CGG + chr1_4:66143655-66143674 None:intergenic 35.0%
AAGTTTATTGCTCGAAGATG+GGG - chr1_4:66143661-66143680 Msa0178740:CDS 35.0%
!! TAGCGGATATTTTATCATGG+CGG - chr1_4:66143719-66143738 Msa0178740:CDS 35.0%
!! GAAGCTTTTGCTTTTACACT+AGG - chr1_4:66144090-66144109 Msa0178740:CDS 35.0%
!! CTTTTGCTTTTACACTAGGT+TGG - chr1_4:66144094-66144113 Msa0178740:CDS 35.0%
!! ACTAGGTTGGTTAATTGTGA+TGG - chr1_4:66144107-66144126 Msa0178740:CDS 35.0%
!! CTAGGTTGGTTAATTGTGAT+GGG - chr1_4:66144108-66144127 Msa0178740:CDS 35.0%
AACCATGAATTACGTCAAAC+TGG - chr1_4:66144193-66144212 Msa0178740:CDS 35.0%
TTCCAGTTTGACGTAATTCA+TGG + chr1_4:66144198-66144217 None:intergenic 35.0%
TTACGTCAAACTGGAAACTT+TGG - chr1_4:66144202-66144221 Msa0178740:CDS 35.0%
TACGTCAAACTGGAAACTTT+GGG - chr1_4:66144203-66144222 Msa0178740:CDS 35.0%
ATTACACTTTCAACTCCTCA+TGG - chr1_4:66144380-66144399 Msa0178740:CDS 35.0%
AAAACACCAATCAGACTTGA+AGG + chr1_4:66144414-66144433 None:intergenic 35.0%
ATTGTACACCTTGTTACCTA+TGG - chr1_4:66144483-66144502 Msa0178740:intron 35.0%
ATGATATCAAGCACATCCAA+AGG + chr1_4:66143468-66143487 None:intergenic 35.0%
! GTCGTCAAAATCTAGTGTTT+TGG + chr1_4:66143493-66143512 None:intergenic 35.0%
ACACACCAGAAAATTGAAAG+TGG + chr1_4:66143515-66143534 None:intergenic 35.0%
CAAATTTCTTGTCAACCTAC+GGG - chr1_4:66143606-66143625 Msa0178740:CDS 35.0%
ATAAAGGAAAATGATCCCGT+AGG + chr1_4:66143624-66143643 None:intergenic 35.0%
!! GAGCAATAAACTTTTTGGTC+GGG + chr1_4:66143654-66143673 None:intergenic 35.0%
!! CGAGCAATAAACTTTTTGGT+CGG + chr1_4:66143655-66143674 None:intergenic 35.0%
AAGTTTATTGCTCGAAGATG+GGG - chr1_4:66143661-66143680 Msa0178740:CDS 35.0%
!! TAGCGGATATTTTATCATGG+CGG - chr1_4:66143719-66143738 Msa0178740:CDS 35.0%
!! GAAGCTTTTGCTTTTACACT+AGG - chr1_4:66144090-66144109 Msa0178740:CDS 35.0%
!! CTTTTGCTTTTACACTAGGT+TGG - chr1_4:66144094-66144113 Msa0178740:CDS 35.0%
!! ACTAGGTTGGTTAATTGTGA+TGG - chr1_4:66144107-66144126 Msa0178740:CDS 35.0%
!! CTAGGTTGGTTAATTGTGAT+GGG - chr1_4:66144108-66144127 Msa0178740:CDS 35.0%
AACCATGAATTACGTCAAAC+TGG - chr1_4:66144193-66144212 Msa0178740:CDS 35.0%
TTCCAGTTTGACGTAATTCA+TGG + chr1_4:66144198-66144217 None:intergenic 35.0%
TTACGTCAAACTGGAAACTT+TGG - chr1_4:66144202-66144221 Msa0178740:CDS 35.0%
TACGTCAAACTGGAAACTTT+GGG - chr1_4:66144203-66144222 Msa0178740:CDS 35.0%
ATTACACTTTCAACTCCTCA+TGG - chr1_4:66144380-66144399 Msa0178740:CDS 35.0%
AAAACACCAATCAGACTTGA+AGG + chr1_4:66144414-66144433 None:intergenic 35.0%
ATTGTACACCTTGTTACCTA+TGG - chr1_4:66144483-66144502 Msa0178740:intron 35.0%
CAAAGGAAAACAGGTGAAGA+TGG - chr1_4:66143422-66143441 Msa0178740:three_prime_UTR 40.0%
! GACGACCACTTTCAATTTTC+TGG - chr1_4:66143507-66143526 Msa0178740:CDS 40.0%
! TTTCTGGTGTGTGCAAGAAT+TGG - chr1_4:66143523-66143542 Msa0178740:CDS 40.0%
TTGGTCGGGTATGCAAATAA+AGG + chr1_4:66143640-66143659 None:intergenic 40.0%
AAATCGTAACATAGGCAGAG+CGG + chr1_4:66143698-66143717 None:intergenic 40.0%
! GCCTATGTTACGATTTCTAG+CGG - chr1_4:66143702-66143721 Msa0178740:CDS 40.0%
TCCGCTAGAAATCGTAACAT+AGG + chr1_4:66143706-66143725 None:intergenic 40.0%
! AGCGGATATTTTATCATGGC+GGG - chr1_4:66143720-66143739 Msa0178740:CDS 40.0%
AACCACCAAGACAAATTCCT+CGG + chr1_4:66143871-66143890 None:intergenic 40.0%
! TTGGGTTGCTTATTCGAGAA+TGG - chr1_4:66143935-66143954 Msa0178740:CDS 40.0%
ACGAAGTCAACAATTACCTC+TGG + chr1_4:66143966-66143985 None:intergenic 40.0%
!! AGAGGTAATTGTTGACTTCG+TGG - chr1_4:66143965-66143984 Msa0178740:CDS 40.0%
!! CTAAAACTGAAGAGTACTCC+TGG - chr1_4:66144068-66144087 Msa0178740:CDS 40.0%
GTGTAAAAGCAAAAGCTTCC+AGG + chr1_4:66144089-66144108 None:intergenic 40.0%
!!! TTGTGATGGGCAACTTTTAG+TGG - chr1_4:66144121-66144140 Msa0178740:CDS 40.0%
AGTTGTAGTGTGTATTCGGT+TGG - chr1_4:66144335-66144354 Msa0178740:CDS 40.0%
! TTGAAGGAACTTGTTCCATG+AGG + chr1_4:66144398-66144417 None:intergenic 40.0%
CAAGTTCCTTCAAGTCTGAT+TGG - chr1_4:66144405-66144424 Msa0178740:CDS 40.0%
AACACAGACCATAGGTAACA+AGG + chr1_4:66144494-66144513 None:intergenic 40.0%
TCGAGCCCTAATCAAACTAA+AGG + chr1_4:66144529-66144548 None:intergenic 40.0%
CAAAGGAAAACAGGTGAAGA+TGG - chr1_4:66143422-66143441 Msa0178740:three_prime_UTR 40.0%
! GACGACCACTTTCAATTTTC+TGG - chr1_4:66143507-66143526 Msa0178740:CDS 40.0%
! TTTCTGGTGTGTGCAAGAAT+TGG - chr1_4:66143523-66143542 Msa0178740:CDS 40.0%
TTGGTCGGGTATGCAAATAA+AGG + chr1_4:66143640-66143659 None:intergenic 40.0%
AAATCGTAACATAGGCAGAG+CGG + chr1_4:66143698-66143717 None:intergenic 40.0%
! GCCTATGTTACGATTTCTAG+CGG - chr1_4:66143702-66143721 Msa0178740:CDS 40.0%
TCCGCTAGAAATCGTAACAT+AGG + chr1_4:66143706-66143725 None:intergenic 40.0%
! AGCGGATATTTTATCATGGC+GGG - chr1_4:66143720-66143739 Msa0178740:CDS 40.0%
AACCACCAAGACAAATTCCT+CGG + chr1_4:66143871-66143890 None:intergenic 40.0%
! TTGGGTTGCTTATTCGAGAA+TGG - chr1_4:66143935-66143954 Msa0178740:CDS 40.0%
ACGAAGTCAACAATTACCTC+TGG + chr1_4:66143966-66143985 None:intergenic 40.0%
!! AGAGGTAATTGTTGACTTCG+TGG - chr1_4:66143965-66143984 Msa0178740:CDS 40.0%
!! CTAAAACTGAAGAGTACTCC+TGG - chr1_4:66144068-66144087 Msa0178740:CDS 40.0%
GTGTAAAAGCAAAAGCTTCC+AGG + chr1_4:66144089-66144108 None:intergenic 40.0%
!!! TTGTGATGGGCAACTTTTAG+TGG - chr1_4:66144121-66144140 Msa0178740:CDS 40.0%
AGTTGTAGTGTGTATTCGGT+TGG - chr1_4:66144335-66144354 Msa0178740:CDS 40.0%
! TTGAAGGAACTTGTTCCATG+AGG + chr1_4:66144398-66144417 None:intergenic 40.0%
CAAGTTCCTTCAAGTCTGAT+TGG - chr1_4:66144405-66144424 Msa0178740:CDS 40.0%
AACACAGACCATAGGTAACA+AGG + chr1_4:66144494-66144513 None:intergenic 40.0%
TCGAGCCCTAATCAAACTAA+AGG + chr1_4:66144529-66144548 None:intergenic 40.0%
GCACATCCAAAGGAAGTTCA+CGG + chr1_4:66143458-66143477 None:intergenic 45.0%
TGGTGTGTGCAAGAATTGGA+GGG - chr1_4:66143527-66143546 Msa0178740:CDS 45.0%
! GCGGATATTTTATCATGGCG+GGG - chr1_4:66143721-66143740 Msa0178740:CDS 45.0%
! GCTTTTGCCAAAAGCAAGCA+AGG + chr1_4:66143847-66143866 None:intergenic 45.0%
AAGCTCCGAGGAATTTGTCT+TGG - chr1_4:66143863-66143882 Msa0178740:CDS 45.0%
TTCGAGAATGGAAGATCCAG+AGG - chr1_4:66143947-66143966 Msa0178740:CDS 45.0%
TCACTGACAAGGCCAACATA+GGG - chr1_4:66144012-66144031 Msa0178740:CDS 45.0%
TTTCATATCCCCACCTGTTG+GGG + chr1_4:66144280-66144299 None:intergenic 45.0%
CTTTCATATCCCCACCTGTT+GGG + chr1_4:66144281-66144300 None:intergenic 45.0%
TAGGTGGAAACACAGACCAT+AGG + chr1_4:66144502-66144521 None:intergenic 45.0%
TAGGGCTCGAAAACTTGTAG+TGG - chr1_4:66144539-66144558 Msa0178740:intron 45.0%
GCACATCCAAAGGAAGTTCA+CGG + chr1_4:66143458-66143477 None:intergenic 45.0%
TGGTGTGTGCAAGAATTGGA+GGG - chr1_4:66143527-66143546 Msa0178740:CDS 45.0%
! GCGGATATTTTATCATGGCG+GGG - chr1_4:66143721-66143740 Msa0178740:CDS 45.0%
! GCTTTTGCCAAAAGCAAGCA+AGG + chr1_4:66143847-66143866 None:intergenic 45.0%
AAGCTCCGAGGAATTTGTCT+TGG - chr1_4:66143863-66143882 Msa0178740:CDS 45.0%
TTCGAGAATGGAAGATCCAG+AGG - chr1_4:66143947-66143966 Msa0178740:CDS 45.0%
TCACTGACAAGGCCAACATA+GGG - chr1_4:66144012-66144031 Msa0178740:CDS 45.0%
TTTCATATCCCCACCTGTTG+GGG + chr1_4:66144280-66144299 None:intergenic 45.0%
CTTTCATATCCCCACCTGTT+GGG + chr1_4:66144281-66144300 None:intergenic 45.0%
TAGGTGGAAACACAGACCAT+AGG + chr1_4:66144502-66144521 None:intergenic 45.0%
TAGGGCTCGAAAACTTGTAG+TGG - chr1_4:66144539-66144558 Msa0178740:intron 45.0%
AGAGAGCCGTGAACTTCCTT+TGG - chr1_4:66143449-66143468 Msa0178740:exon 50.0%
CTGGTGTGTGCAAGAATTGG+AGG - chr1_4:66143526-66143545 Msa0178740:CDS 50.0%
! AGACGTGCCTTGCTTGCTTT+TGG - chr1_4:66143837-66143856 Msa0178740:CDS 50.0%
! TGCTTTTGGCAAAAGCTCCG+AGG - chr1_4:66143851-66143870 Msa0178740:CDS 50.0%
CTCCGAGGAATTTGTCTTGG+TGG - chr1_4:66143866-66143885 Msa0178740:CDS 50.0%
GTCACTGACAAGGCCAACAT+AGG - chr1_4:66144011-66144030 Msa0178740:CDS 50.0%
CACTGACAAGGCCAACATAG+GGG - chr1_4:66144013-66144032 Msa0178740:CDS 50.0%
ATGCATTGAGCAACCCCAAC+AGG - chr1_4:66144264-66144283 Msa0178740:CDS 50.0%
ATTGAGCAACCCCAACAGGT+GGG - chr1_4:66144268-66144287 Msa0178740:CDS 50.0%
GCTTTCATATCCCCACCTGT+TGG + chr1_4:66144282-66144301 None:intergenic 50.0%
AGAGAGCCGTGAACTTCCTT+TGG - chr1_4:66143449-66143468 Msa0178740:exon 50.0%
CTGGTGTGTGCAAGAATTGG+AGG - chr1_4:66143526-66143545 Msa0178740:CDS 50.0%
! AGACGTGCCTTGCTTGCTTT+TGG - chr1_4:66143837-66143856 Msa0178740:CDS 50.0%
! TGCTTTTGGCAAAAGCTCCG+AGG - chr1_4:66143851-66143870 Msa0178740:CDS 50.0%
CTCCGAGGAATTTGTCTTGG+TGG - chr1_4:66143866-66143885 Msa0178740:CDS 50.0%
GTCACTGACAAGGCCAACAT+AGG - chr1_4:66144011-66144030 Msa0178740:CDS 50.0%
CACTGACAAGGCCAACATAG+GGG - chr1_4:66144013-66144032 Msa0178740:CDS 50.0%
ATGCATTGAGCAACCCCAAC+AGG - chr1_4:66144264-66144283 Msa0178740:CDS 50.0%
ATTGAGCAACCCCAACAGGT+GGG - chr1_4:66144268-66144287 Msa0178740:CDS 50.0%
GCTTTCATATCCCCACCTGT+TGG + chr1_4:66144282-66144301 None:intergenic 50.0%
CAAGCTGAGCACCCCTATGT+TGG + chr1_4:66144027-66144046 None:intergenic 55.0%
CATTGAGCAACCCCAACAGG+TGG - chr1_4:66144267-66144286 Msa0178740:CDS 55.0%
TTGAGCAACCCCAACAGGTG+GGG - chr1_4:66144269-66144288 Msa0178740:CDS 55.0%
CAAGCTGAGCACCCCTATGT+TGG + chr1_4:66144027-66144046 None:intergenic 55.0%
CATTGAGCAACCCCAACAGG+TGG - chr1_4:66144267-66144286 Msa0178740:CDS 55.0%
TTGAGCAACCCCAACAGGTG+GGG - chr1_4:66144269-66144288 Msa0178740:CDS 55.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 66143279 66144647 66143279 ID=Msa0178740;Name=Msa0178740
chr1_4 mRNA 66143279 66144647 66143279 ID=Msa0178740-mRNA-1;Parent=Msa0178740;Name=Msa0178740-mRNA-1;_AED=0.24;_eAED=0.24;_QI=0|0|0.5|0.5|0|0|2|172|363
chr1_4 exon 66143279 66144499 66143279 ID=Msa0178740-mRNA-1:exon:21603;Parent=Msa0178740-mRNA-1
chr1_4 exon 66144605 66144647 66144605 ID=Msa0178740-mRNA-1:exon:21602;Parent=Msa0178740-mRNA-1
chr1_4 CDS 66144605 66144647 66144605 ID=Msa0178740-mRNA-1:cds;Parent=Msa0178740-mRNA-1
chr1_4 CDS 66143451 66144499 66143451 ID=Msa0178740-mRNA-1:cds;Parent=Msa0178740-mRNA-1
chr1_4 three_prime_UTR 66143279 66143450 66143279 ID=Msa0178740-mRNA-1:three_prime_utr;Parent=Msa0178740-mRNA-1
Gene Sequence

>Msa0178740

ATGTATTTTACGTTGATATGGATTTTTGTTTGGGGAAAATGCGGAAAACAGGTGAAGATGGTACAAGAGAGCCGTGAACTTCCTTTGGATGTGCTTGATATCATTTCCAAAACACTAGATTTTGACGACCACTTTCAATTTTCTGGTGTGTGCAAGAATTGGAGGGCTTTTCATAAAATTTACTCTAGAAATTTCTTGGCATCCCAAGAACCTTTACTTCTTCAAATTTCTTGTCAACCTACGGGATCATTTTCCTTTATTTGCATACCCGACCAAAAAGTTTATTGCTCGAAGATGGGGAAGTACTTCTTCCGCTCTGCCTATGTTACGATTTCTAGCGGATATTTTATCATGGCGGGGTATGATAATTCATTTATGCTATTGAATCCATTTACAAGAATAAAAAAGGTGGTCAATACCCCTATTTCTAAAGTTAAAGGTTCTTATTTAACTAGACGTGCCTTGCTTGCTTTTGGCAAAAGCTCCGAGGAATTTGTCTTGGTGGTTTTATGCAATTATTGTTTGCATGTCTATCAATCTCGAAATTTAGATTGGGTTGCTTATTCGAGAATGGAAGATCCAGAGGTAATTGTTGACTTCGTGGTTTTGCATAATGTAATATATGTTGTCACTGACAAGGCCAACATAGGGGTGCTCAGCTTGAATTCTGCAAGTATTAAATTTCTAAAACTGAAGAGTACTCCTGGAAGCTTTTGCTTTTACACTAGGTTGGTTAATTGTGATGGGCAACTTTTAGTGGTTGATTTAACGTCCAATAAAATATGTAATGTGTACAAGATAGACTTCTCAACCATGAATTACGTCAAACTGGAAACTTTGGGCGACATTGCATTATTTTATGTTTCATACAGAAGTTGTTATGCATTGAGCAACCCCAACAGGTGGGGATATGAAAGCAACTCTGTGTATATCATCAGTTCTCTATCTTCAAGTTGTAGTGTGTATTCGGTTGGTGATAAAAAATTGCAAAAGAACATTACACTTTCAACTCCTCATGGAACAAGTTCCTTCAAGTCTGATTGGTGTTTTAGACATCAACATTTTGAGGTAGATTATTCTCTAGTTGAGTGA
Protein sequence

>Msa0178740

MYFTLIWIFVWGKCGKQVKMVQESRELPLDVLDIISKTLDFDDHFQFSGVCKNWRAFHKIYSRNFLASQEPLLLQISCQPTGSFSFICIPDQKVYCSKMGKYFFRSAYVTISSGYFIMAGYDNSFMLLNPFTRIKKVVNTPISKVKGSYLTRRALLAFGKSSEEFVLVVLCNYCLHVYQSRNLDWVAYSRMEDPEVIVDFVVLHNVIYVVTDKANIGVLSLNSASIKFLKLKSTPGSFCFYTRLVNCDGQLLVVDLTSNKICNVYKIDFSTMNYVKLETLGDIALFYVSYRSCYALSNPNRWGYESNSVYIISSLSSSCSVYSVGDKKLQKNITLSTPHGTSSFKSDWCFRHQHFEVDYSLVE*