AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1144450


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1144450 MtrunA17_Chr5g0393501 47.907 215 110 1 2 216 29 241 3.06e-65 202
Msa1144450 MtrunA17_Chr4g0044671 25.500 200 142 2 7 204 15 209 5.19e-12 63.2
Msa1144450 MtrunA17_Chr4g0034781 26.000 200 141 2 7 204 9 203 5.75e-12 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 35 sgRNAs with CRISPR-Local

Find 102 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGCCTAATATTGATGAATT+TGG 0.071532 7_4:+20491209 Msa1144450:CDS
TATAAGGCATTGTTAAAATT+TGG 0.194239 7_4:-20490998 None:intergenic
AAGAATTCCGCAATGAAAAT+TGG 0.202897 7_4:-20490909 None:intergenic
TGATGAATTTGGGACAAAAT+TGG 0.234698 7_4:+20491220 Msa1144450:CDS
TACTATGAAATCAATGATTA+TGG 0.240311 7_4:+20491050 Msa1144450:CDS
CATCTTCCTTTCATTCATAA+TGG 0.274505 7_4:+20490745 Msa1144450:exon
GTCCCAAATTCATCAATATT+AGG 0.280924 7_4:-20491212 None:intergenic
GTTTAAGTGTGACTCAATTA+TGG 0.303074 7_4:+20491524 Msa1144450:CDS
TGCCTAATATTGATGAATTT+GGG 0.308974 7_4:+20491210 Msa1144450:CDS
CTAAATATGTAAAAGATATT+AGG 0.309400 7_4:-20491073 None:intergenic
ACCGTGGAAATTCGTTTGTT+TGG 0.318850 7_4:-20491136 None:intergenic
TCCCAACAAAAGGTAATATA+AGG 0.319342 7_4:-20491014 None:intergenic
TTGCATACAGTGATGAAATA+AGG 0.320941 7_4:-20490779 None:intergenic
GGAAATTCGTTTGTTTGGAT+TGG 0.329638 7_4:-20491131 None:intergenic
GAAATTCGTTTGTTTGGATT+GGG 0.339538 7_4:-20491130 None:intergenic
TGCGGAATTCTTCAATGAAC+TGG 0.345003 7_4:+20490920 Msa1144450:CDS
TTCCACATACACGTGTTTCA+TGG 0.348799 7_4:+20491107 Msa1144450:CDS
TTAAACTCTAAAATATCATT+TGG 0.369978 7_4:-20491507 None:intergenic
CAGATCTTACCAAATGACTT+TGG 0.384038 7_4:+20491321 Msa1144450:intron
TGCGAGTTATAGATGTAGTT+TGG 0.385150 7_4:-20491166 None:intergenic
CAATGAACTGGATGAACAAT+GGG 0.420513 7_4:+20490932 Msa1144450:CDS
ACACAAACTATTGTTAAGAT+AGG 0.424067 7_4:+20491444 Msa1144450:CDS
AATATTACAATACTTAAAAC+AGG 0.460074 7_4:-20491593 None:intergenic
TCCCAATGATTCCCAACAAA+AGG 0.467567 7_4:-20491024 None:intergenic
CATTCTCATTTACGATTGTG+TGG 0.506907 7_4:+20491366 Msa1144450:CDS
AGTGATGAAATAAGGTCTGT+TGG 0.517513 7_4:-20490771 None:intergenic
CATTATGAATGAAAGGAAGA+TGG 0.523264 7_4:-20490744 None:intergenic
TTGTTAAGATAGGACGTAGC+TGG 0.529092 7_4:+20491454 Msa1144450:CDS
GGTTAAATACAACAATGTGT+TGG 0.550805 7_4:-20490977 None:intergenic
TCAATGAACTGGATGAACAA+TGG 0.558244 7_4:+20490931 Msa1144450:CDS
GGCCATGAAACACGTGTATG+TGG 0.560867 7_4:-20491109 None:intergenic
TGGAGTCCATTATGAATGAA+AGG 0.569698 7_4:-20490751 None:intergenic
GGAATGGTGATAATTAGTAA+TGG 0.583941 7_4:-20491653 None:intergenic
TCCAAACAAACGAATTTCCA+CGG 0.596587 7_4:+20491135 Msa1144450:CDS
GTAGTTTGGCTATGATACCG+TGG 0.697972 7_4:-20491152 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATATTATTTTTTAGCATAA+AGG - chr7_4:20491484-20491503 None:intergenic 10.0%
!!! AATATTATTTTTTAGCATAA+AGG - chr7_4:20491484-20491503 None:intergenic 10.0%
!! CTAAATATGTAAAAGATATT+AGG - chr7_4:20491076-20491095 None:intergenic 15.0%
!!! CTAATATCTTTTACATATTT+AGG + chr7_4:20491074-20491093 Msa1144450:CDS 15.0%
!!! TTAAACTCTAAAATATCATT+TGG - chr7_4:20491510-20491529 None:intergenic 15.0%
!! AATATTACAATACTTAAAAC+AGG - chr7_4:20491596-20491615 None:intergenic 15.0%
!! CTAAATATGTAAAAGATATT+AGG - chr7_4:20491076-20491095 None:intergenic 15.0%
!!! CTAATATCTTTTACATATTT+AGG + chr7_4:20491074-20491093 Msa1144450:CDS 15.0%
!!! TTAAACTCTAAAATATCATT+TGG - chr7_4:20491510-20491529 None:intergenic 15.0%
!! AATATTACAATACTTAAAAC+AGG - chr7_4:20491596-20491615 None:intergenic 15.0%
!!! TATAAGGCATTGTTAAAATT+TGG - chr7_4:20491001-20491020 None:intergenic 20.0%
!! TACTATGAAATCAATGATTA+TGG + chr7_4:20491050-20491069 Msa1144450:CDS 20.0%
!!! TATAAGGCATTGTTAAAATT+TGG - chr7_4:20491001-20491020 None:intergenic 20.0%
!! TACTATGAAATCAATGATTA+TGG + chr7_4:20491050-20491069 Msa1144450:CDS 20.0%
! ATGCCTAATATTGATGAATT+TGG + chr7_4:20491209-20491228 Msa1144450:CDS 25.0%
! TGCCTAATATTGATGAATTT+GGG + chr7_4:20491210-20491229 Msa1144450:CDS 25.0%
!! ACAACTGTCTTATATTTTTC+TGG + chr7_4:20491252-20491271 Msa1144450:intron 25.0%
! AAAAAAGATCCAAAGTCATT+TGG - chr7_4:20491333-20491352 None:intergenic 25.0%
! ACACAAACTATTGTTAAGAT+AGG + chr7_4:20491444-20491463 Msa1144450:CDS 25.0%
! ATGCCTAATATTGATGAATT+TGG + chr7_4:20491209-20491228 Msa1144450:CDS 25.0%
! TGCCTAATATTGATGAATTT+GGG + chr7_4:20491210-20491229 Msa1144450:CDS 25.0%
!! ACAACTGTCTTATATTTTTC+TGG + chr7_4:20491252-20491271 Msa1144450:intron 25.0%
! AAAAAAGATCCAAAGTCATT+TGG - chr7_4:20491333-20491352 None:intergenic 25.0%
! ACACAAACTATTGTTAAGAT+AGG + chr7_4:20491444-20491463 Msa1144450:CDS 25.0%
CATTATGAATGAAAGGAAGA+TGG - chr7_4:20490747-20490766 None:intergenic 30.0%
CATCTTCCTTTCATTCATAA+TGG + chr7_4:20490745-20490764 Msa1144450:exon 30.0%
TTGCATACAGTGATGAAATA+AGG - chr7_4:20490782-20490801 None:intergenic 30.0%
CACTGTATGCAAAAAAAATC+AGG + chr7_4:20490790-20490809 Msa1144450:CDS 30.0%
AAGAATTCCGCAATGAAAAT+TGG - chr7_4:20490912-20490931 None:intergenic 30.0%
GGTTAAATACAACAATGTGT+TGG - chr7_4:20490980-20490999 None:intergenic 30.0%
! TGCCTTATATTACCTTTTGT+TGG + chr7_4:20491012-20491031 Msa1144450:CDS 30.0%
! GCCTTATATTACCTTTTGTT+GGG + chr7_4:20491013-20491032 Msa1144450:CDS 30.0%
TCCCAACAAAAGGTAATATA+AGG - chr7_4:20491017-20491036 None:intergenic 30.0%
GAAATTCGTTTGTTTGGATT+GGG - chr7_4:20491133-20491152 None:intergenic 30.0%
GCATAAAAACCACAAAAGTT+GGG - chr7_4:20491193-20491212 None:intergenic 30.0%
GTCCCAAATTCATCAATATT+AGG - chr7_4:20491215-20491234 None:intergenic 30.0%
! TGATGAATTTGGGACAAAAT+TGG + chr7_4:20491220-20491239 Msa1144450:CDS 30.0%
!!! GACTTTGGATCTTTTTTGAA+AGG + chr7_4:20491336-20491355 Msa1144450:CDS 30.0%
GTTTAAGTGTGACTCAATTA+TGG + chr7_4:20491524-20491543 Msa1144450:CDS 30.0%
CATTATGAATGAAAGGAAGA+TGG - chr7_4:20490747-20490766 None:intergenic 30.0%
CATCTTCCTTTCATTCATAA+TGG + chr7_4:20490745-20490764 Msa1144450:exon 30.0%
TTGCATACAGTGATGAAATA+AGG - chr7_4:20490782-20490801 None:intergenic 30.0%
CACTGTATGCAAAAAAAATC+AGG + chr7_4:20490790-20490809 Msa1144450:CDS 30.0%
AAGAATTCCGCAATGAAAAT+TGG - chr7_4:20490912-20490931 None:intergenic 30.0%
GGTTAAATACAACAATGTGT+TGG - chr7_4:20490980-20490999 None:intergenic 30.0%
! TGCCTTATATTACCTTTTGT+TGG + chr7_4:20491012-20491031 Msa1144450:CDS 30.0%
! GCCTTATATTACCTTTTGTT+GGG + chr7_4:20491013-20491032 Msa1144450:CDS 30.0%
TCCCAACAAAAGGTAATATA+AGG - chr7_4:20491017-20491036 None:intergenic 30.0%
GAAATTCGTTTGTTTGGATT+GGG - chr7_4:20491133-20491152 None:intergenic 30.0%
GCATAAAAACCACAAAAGTT+GGG - chr7_4:20491193-20491212 None:intergenic 30.0%
GTCCCAAATTCATCAATATT+AGG - chr7_4:20491215-20491234 None:intergenic 30.0%
! TGATGAATTTGGGACAAAAT+TGG + chr7_4:20491220-20491239 Msa1144450:CDS 30.0%
!!! GACTTTGGATCTTTTTTGAA+AGG + chr7_4:20491336-20491355 Msa1144450:CDS 30.0%
GTTTAAGTGTGACTCAATTA+TGG + chr7_4:20491524-20491543 Msa1144450:CDS 30.0%
TGGAGTCCATTATGAATGAA+AGG - chr7_4:20490754-20490773 None:intergenic 35.0%
AGTGATGAAATAAGGTCTGT+TGG - chr7_4:20490774-20490793 None:intergenic 35.0%
! GCTTGATCCAATTTTCATTG+CGG + chr7_4:20490902-20490921 Msa1144450:CDS 35.0%
TCAATGAACTGGATGAACAA+TGG + chr7_4:20490931-20490950 Msa1144450:CDS 35.0%
CAATGAACTGGATGAACAAT+GGG + chr7_4:20490932-20490951 Msa1144450:CDS 35.0%
! TACCTTTTGTTGGGAATCAT+TGG + chr7_4:20491022-20491041 Msa1144450:CDS 35.0%
! ACCTTTTGTTGGGAATCATT+GGG + chr7_4:20491023-20491042 Msa1144450:CDS 35.0%
GGAAATTCGTTTGTTTGGAT+TGG - chr7_4:20491134-20491153 None:intergenic 35.0%
TCCAAACAAACGAATTTCCA+CGG + chr7_4:20491135-20491154 Msa1144450:CDS 35.0%
TGCGAGTTATAGATGTAGTT+TGG - chr7_4:20491169-20491188 None:intergenic 35.0%
GGCATAAAAACCACAAAAGT+TGG - chr7_4:20491194-20491213 None:intergenic 35.0%
GATCTGCAAGAAGGAAAAAA+TGG - chr7_4:20491307-20491326 None:intergenic 35.0%
ATTTGGTAAGATCTGCAAGA+AGG - chr7_4:20491316-20491335 None:intergenic 35.0%
CAGATCTTACCAAATGACTT+TGG + chr7_4:20491321-20491340 Msa1144450:intron 35.0%
CATTCTCATTTACGATTGTG+TGG + chr7_4:20491366-20491385 Msa1144450:CDS 35.0%
TGGAGTCCATTATGAATGAA+AGG - chr7_4:20490754-20490773 None:intergenic 35.0%
AGTGATGAAATAAGGTCTGT+TGG - chr7_4:20490774-20490793 None:intergenic 35.0%
! GCTTGATCCAATTTTCATTG+CGG + chr7_4:20490902-20490921 Msa1144450:CDS 35.0%
TCAATGAACTGGATGAACAA+TGG + chr7_4:20490931-20490950 Msa1144450:CDS 35.0%
CAATGAACTGGATGAACAAT+GGG + chr7_4:20490932-20490951 Msa1144450:CDS 35.0%
! TACCTTTTGTTGGGAATCAT+TGG + chr7_4:20491022-20491041 Msa1144450:CDS 35.0%
! ACCTTTTGTTGGGAATCATT+GGG + chr7_4:20491023-20491042 Msa1144450:CDS 35.0%
GGAAATTCGTTTGTTTGGAT+TGG - chr7_4:20491134-20491153 None:intergenic 35.0%
TCCAAACAAACGAATTTCCA+CGG + chr7_4:20491135-20491154 Msa1144450:CDS 35.0%
TGCGAGTTATAGATGTAGTT+TGG - chr7_4:20491169-20491188 None:intergenic 35.0%
GGCATAAAAACCACAAAAGT+TGG - chr7_4:20491194-20491213 None:intergenic 35.0%
GATCTGCAAGAAGGAAAAAA+TGG - chr7_4:20491307-20491326 None:intergenic 35.0%
ATTTGGTAAGATCTGCAAGA+AGG - chr7_4:20491316-20491335 None:intergenic 35.0%
CAGATCTTACCAAATGACTT+TGG + chr7_4:20491321-20491340 Msa1144450:intron 35.0%
CATTCTCATTTACGATTGTG+TGG + chr7_4:20491366-20491385 Msa1144450:CDS 35.0%
TGCGGAATTCTTCAATGAAC+TGG + chr7_4:20490920-20490939 Msa1144450:CDS 40.0%
TCCCAATGATTCCCAACAAA+AGG - chr7_4:20491027-20491046 None:intergenic 40.0%
TTCCACATACACGTGTTTCA+TGG + chr7_4:20491107-20491126 Msa1144450:CDS 40.0%
ACCGTGGAAATTCGTTTGTT+TGG - chr7_4:20491139-20491158 None:intergenic 40.0%
TTGTTAAGATAGGACGTAGC+TGG + chr7_4:20491454-20491473 Msa1144450:CDS 40.0%
TGCGGAATTCTTCAATGAAC+TGG + chr7_4:20490920-20490939 Msa1144450:CDS 40.0%
TCCCAATGATTCCCAACAAA+AGG - chr7_4:20491027-20491046 None:intergenic 40.0%
TTCCACATACACGTGTTTCA+TGG + chr7_4:20491107-20491126 Msa1144450:CDS 40.0%
ACCGTGGAAATTCGTTTGTT+TGG - chr7_4:20491139-20491158 None:intergenic 40.0%
TTGTTAAGATAGGACGTAGC+TGG + chr7_4:20491454-20491473 Msa1144450:CDS 40.0%
GTAGTTTGGCTATGATACCG+TGG - chr7_4:20491155-20491174 None:intergenic 45.0%
! AACTCGCAACCCAACTTTTG+TGG + chr7_4:20491181-20491200 Msa1144450:CDS 45.0%
GTAGTTTGGCTATGATACCG+TGG - chr7_4:20491155-20491174 None:intergenic 45.0%
! AACTCGCAACCCAACTTTTG+TGG + chr7_4:20491181-20491200 Msa1144450:CDS 45.0%
GATCAAGCTCAGCACTCATC+TGG - chr7_4:20490890-20490909 None:intergenic 50.0%
GGCCATGAAACACGTGTATG+TGG - chr7_4:20491112-20491131 None:intergenic 50.0%
GATCAAGCTCAGCACTCATC+TGG - chr7_4:20490890-20490909 None:intergenic 50.0%
GGCCATGAAACACGTGTATG+TGG - chr7_4:20491112-20491131 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
chr7_4 gene 20490727 20491661 20490727 ID=Msa1144450;Name=Msa1144450
chr7_4 mRNA 20490727 20491661 20490727 ID=Msa1144450-mRNA-1;Parent=Msa1144450;Name=Msa1144450-mRNA-1;_AED=0.05;_eAED=0.05;_QI=37|1|1|1|0|0|3|59|225
chr7_4 exon 20490727 20490811 20490727 ID=Msa1144450-mRNA-1:exon:4917;Parent=Msa1144450-mRNA-1
chr7_4 exon 20490891 20491241 20490891 ID=Msa1144450-mRNA-1:exon:4918;Parent=Msa1144450-mRNA-1
chr7_4 exon 20491324 20491661 20491324 ID=Msa1144450-mRNA-1:exon:4919;Parent=Msa1144450-mRNA-1
chr7_4 five_prime_UTR 20490727 20490763 20490727 ID=Msa1144450-mRNA-1:five_prime_utr;Parent=Msa1144450-mRNA-1
chr7_4 CDS 20490764 20490811 20490764 ID=Msa1144450-mRNA-1:cds;Parent=Msa1144450-mRNA-1
chr7_4 CDS 20490891 20491241 20490891 ID=Msa1144450-mRNA-1:cds;Parent=Msa1144450-mRNA-1
chr7_4 CDS 20491324 20491602 20491324 ID=Msa1144450-mRNA-1:cds;Parent=Msa1144450-mRNA-1
chr7_4 three_prime_UTR 20491603 20491661 20491603 ID=Msa1144450-mRNA-1:three_prime_utr;Parent=Msa1144450-mRNA-1
Gene Sequence

>Msa1144450

ATGGACTCCAACAGACCTTATTTCATCACTGTATGCAAAAAAAATCAGATGAGTGCTGAGCTTGATCCAATTTTCATTGCGGAATTCTTCAATGAACTGGATGAACAATGGGTCTTGATTGATGAAAATGAGAACCAACACATTGTTGTATTTAACCAAATTTTAACAATGCCTTATATTACCTTTTGTTGGGAATCATTGGGAATTTACTATGAAATCAATGATTATGGCCTAATATCTTTTACATATTTAGGTGAAAGTAAGTTCCACATACACGTGTTTCATGGCCCAATCCAAACAAACGAATTTCCACGGTATCATAGCCAAACTACATCTATAACTCGCAACCCAACTTTTGTGGTTTTTATGCCTAATATTGATGAATTTGGGACAAAATTGATCTTACCAAATGACTTTGGATCTTTTTTGAAAGGAAAAAAACATTCTCATTTACGATTGTGTGGATCGTCAAATTCTATCACAACTTGCAAGTTGTACTTTGAAGATAATCAACAACAAACACAAACTATTGTTAAGATAGGACGTAGCTGGACTACCTTTATGCTAAAAAATAATATTTATCCAAATGATATTTTAGAGTTTAAGTGTGACTCAATTATGGCAACAAATATTGTCATTGTAATCAAGTATGATGAAAGTTCAAGACACCTGTTTTAA

Protein sequence

>Msa1144450

MDSNRPYFITVCKKNQMSAELDPIFIAEFFNELDEQWVLIDENENQHIVVFNQILTMPYITFCWESLGIYYEINDYGLISFTYLGESKFHIHVFHGPIQTNEFPRYHSQTTSITRNPTFVVFMPNIDEFGTKLILPNDFGSFLKGKKHSHLRLCGSSNSITTCKLYFEDNQQQTQTIVKIGRSWTTFMLKNNIYPNDILEFKCDSIMATNIVIVIKYDESSRHLF*