AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0980200


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0980200 MtrunA17_Chr5g0393501 46.099 141 74 1 21 161 21 159 5.69e-35 123
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 33 sgRNAs with CRISPR-Local

Find 96 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGCCTAATATTGATGAATT+TGG 0.071532 6_4:-18172562 Msa0980200:CDS
TATAAGGCATTGTTAAAATT+TGG 0.194239 6_4:+18172773 None:intergenic
AAGAATTCCGCAATGAAAAT+TGG 0.202897 6_4:+18172862 None:intergenic
TGATGAATTTGGGACAAAAT+TGG 0.234698 6_4:-18172551 Msa0980200:CDS
TACTATGAAATCAATGATTA+TGG 0.240311 6_4:-18172721 Msa0980200:CDS
CATCTTCCTTTCATTCATAA+TGG 0.274505 6_4:-18173026 Msa0980200:CDS
GTCCCAAATTCATCAATATT+AGG 0.280924 6_4:+18172559 None:intergenic
TGCCTAATATTGATGAATTT+GGG 0.308974 6_4:-18172561 Msa0980200:CDS
CTAAATATGTAAAAGATATT+AGG 0.309400 6_4:+18172698 None:intergenic
ACCGTGGAAATTCGTTTGTT+TGG 0.318850 6_4:+18172635 None:intergenic
TCCCAACAAAAGGTAATATA+AGG 0.319342 6_4:+18172757 None:intergenic
TTGCATACAGTGATGAAATA+AGG 0.320941 6_4:+18172992 None:intergenic
GGAAATTCGTTTGTTTGGAT+TGG 0.329638 6_4:+18172640 None:intergenic
GAAATTCGTTTGTTTGGATT+GGG 0.339538 6_4:+18172641 None:intergenic
TGCGGAATTCTTCAATGAAC+TGG 0.345003 6_4:-18172851 Msa0980200:CDS
TTCCACATACACGTGTTTCA+TGG 0.348799 6_4:-18172664 Msa0980200:CDS
CAGATCTTACCAAATGACTT+TGG 0.384038 6_4:-18172450 Msa0980200:exon
TGCGAGTTATAGATGTAGTT+TGG 0.385150 6_4:+18172605 None:intergenic
CAATGAACTGGATGAACAAT+GGG 0.420513 6_4:-18172839 Msa0980200:CDS
ACACAAACTATTGTTAAGAT+AGG 0.424067 6_4:-18172327 Msa0980200:three_prime_UTR
TCCCAATGATTCCCAACAAA+AGG 0.467567 6_4:+18172747 None:intergenic
CATTCTCATTTACGATTGTG+TGG 0.506907 6_4:-18172405 Msa0980200:three_prime_UTR
AGTGATGAAATAAGGTCTGT+TGG 0.517513 6_4:+18173000 None:intergenic
CATTATGAATGAAAGGAAGA+TGG 0.523264 6_4:+18173027 None:intergenic
TTGTTAAGATAGGACGTAGC+TGG 0.529092 6_4:-18172317 Msa0980200:three_prime_UTR
GGTTAAATACAACAATGTGT+TGG 0.550805 6_4:+18172794 None:intergenic
TCAATGAACTGGATGAACAA+TGG 0.558244 6_4:-18172840 Msa0980200:CDS
GGCCATGAAACACGTGTATG+TGG 0.560867 6_4:+18172662 None:intergenic
TGGAGTCCATTATGAATGAA+AGG 0.569698 6_4:+18173020 None:intergenic
TCCAAACAAACGAATTTCCA+CGG 0.596587 6_4:-18172636 Msa0980200:CDS
ATTTGGTAAGATCTGCAAGA+AGG 0.603404 6_4:+18172458 None:intergenic
GTGTTGCAAAGATTTACTTG+AGG 0.660916 6_4:+18173060 None:intergenic
GTAGTTTGGCTATGATACCG+TGG 0.697972 6_4:+18172619 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CTAAATATGTAAAAGATATT+AGG + chr6_4:18172689-18172708 None:intergenic 15.0%
!!! CTAATATCTTTTACATATTT+AGG - chr6_4:18172687-18172706 Msa0980200:CDS 15.0%
!! CTAAATATGTAAAAGATATT+AGG + chr6_4:18172689-18172708 None:intergenic 15.0%
!!! CTAATATCTTTTACATATTT+AGG - chr6_4:18172687-18172706 Msa0980200:CDS 15.0%
!!! TATAAGGCATTGTTAAAATT+TGG + chr6_4:18172614-18172633 None:intergenic 20.0%
!! TACTATGAAATCAATGATTA+TGG - chr6_4:18172663-18172682 Msa0980200:CDS 20.0%
!!! TATAAGGCATTGTTAAAATT+TGG + chr6_4:18172614-18172633 None:intergenic 20.0%
!! TACTATGAAATCAATGATTA+TGG - chr6_4:18172663-18172682 Msa0980200:CDS 20.0%
! ATGCCTAATATTGATGAATT+TGG - chr6_4:18172822-18172841 Msa0980200:CDS 25.0%
! TGCCTAATATTGATGAATTT+GGG - chr6_4:18172823-18172842 Msa0980200:CDS 25.0%
!! ACAACTGTCTTATATTTTTC+TGG - chr6_4:18172865-18172884 Msa0980200:CDS 25.0%
! AAAAAAGATCCAAAGTCATT+TGG + chr6_4:18172946-18172965 None:intergenic 25.0%
! ACACAAACTATTGTTAAGAT+AGG - chr6_4:18173057-18173076 Msa0980200:CDS 25.0%
! ATGCCTAATATTGATGAATT+TGG - chr6_4:18172822-18172841 Msa0980200:CDS 25.0%
! TGCCTAATATTGATGAATTT+GGG - chr6_4:18172823-18172842 Msa0980200:CDS 25.0%
!! ACAACTGTCTTATATTTTTC+TGG - chr6_4:18172865-18172884 Msa0980200:CDS 25.0%
! AAAAAAGATCCAAAGTCATT+TGG + chr6_4:18172946-18172965 None:intergenic 25.0%
! ACACAAACTATTGTTAAGAT+AGG - chr6_4:18173057-18173076 Msa0980200:CDS 25.0%
CATTATGAATGAAAGGAAGA+TGG + chr6_4:18172360-18172379 None:intergenic 30.0%
CATCTTCCTTTCATTCATAA+TGG - chr6_4:18172358-18172377 Msa0980200:three_prime_UTR 30.0%
TTGCATACAGTGATGAAATA+AGG + chr6_4:18172395-18172414 None:intergenic 30.0%
CACTGTATGCAAAAAAAATC+AGG - chr6_4:18172403-18172422 Msa0980200:three_prime_UTR 30.0%
AAGAATTCCGCAATGAAAAT+TGG + chr6_4:18172525-18172544 None:intergenic 30.0%
GGTTAAATACAACAATGTGT+TGG + chr6_4:18172593-18172612 None:intergenic 30.0%
! TGCCTTATATTACCTTTTGT+TGG - chr6_4:18172625-18172644 Msa0980200:CDS 30.0%
! GCCTTATATTACCTTTTGTT+GGG - chr6_4:18172626-18172645 Msa0980200:CDS 30.0%
TCCCAACAAAAGGTAATATA+AGG + chr6_4:18172630-18172649 None:intergenic 30.0%
GAAATTCGTTTGTTTGGATT+GGG + chr6_4:18172746-18172765 None:intergenic 30.0%
GCATAAAAACCACAAAAGTT+GGG + chr6_4:18172806-18172825 None:intergenic 30.0%
GTCCCAAATTCATCAATATT+AGG + chr6_4:18172828-18172847 None:intergenic 30.0%
! TGATGAATTTGGGACAAAAT+TGG - chr6_4:18172833-18172852 Msa0980200:CDS 30.0%
!!! GACTTTGGATCTTTTTTGAA+AGG - chr6_4:18172949-18172968 Msa0980200:intron 30.0%
CATTATGAATGAAAGGAAGA+TGG + chr6_4:18172360-18172379 None:intergenic 30.0%
CATCTTCCTTTCATTCATAA+TGG - chr6_4:18172358-18172377 Msa0980200:three_prime_UTR 30.0%
TTGCATACAGTGATGAAATA+AGG + chr6_4:18172395-18172414 None:intergenic 30.0%
CACTGTATGCAAAAAAAATC+AGG - chr6_4:18172403-18172422 Msa0980200:three_prime_UTR 30.0%
AAGAATTCCGCAATGAAAAT+TGG + chr6_4:18172525-18172544 None:intergenic 30.0%
GGTTAAATACAACAATGTGT+TGG + chr6_4:18172593-18172612 None:intergenic 30.0%
! TGCCTTATATTACCTTTTGT+TGG - chr6_4:18172625-18172644 Msa0980200:CDS 30.0%
! GCCTTATATTACCTTTTGTT+GGG - chr6_4:18172626-18172645 Msa0980200:CDS 30.0%
TCCCAACAAAAGGTAATATA+AGG + chr6_4:18172630-18172649 None:intergenic 30.0%
GAAATTCGTTTGTTTGGATT+GGG + chr6_4:18172746-18172765 None:intergenic 30.0%
GCATAAAAACCACAAAAGTT+GGG + chr6_4:18172806-18172825 None:intergenic 30.0%
GTCCCAAATTCATCAATATT+AGG + chr6_4:18172828-18172847 None:intergenic 30.0%
! TGATGAATTTGGGACAAAAT+TGG - chr6_4:18172833-18172852 Msa0980200:CDS 30.0%
!!! GACTTTGGATCTTTTTTGAA+AGG - chr6_4:18172949-18172968 Msa0980200:intron 30.0%
GTGTTGCAAAGATTTACTTG+AGG + chr6_4:18172327-18172346 None:intergenic 35.0%
TGGAGTCCATTATGAATGAA+AGG + chr6_4:18172367-18172386 None:intergenic 35.0%
AGTGATGAAATAAGGTCTGT+TGG + chr6_4:18172387-18172406 None:intergenic 35.0%
! GCTTGATCCAATTTTCATTG+CGG - chr6_4:18172515-18172534 Msa0980200:CDS 35.0%
TCAATGAACTGGATGAACAA+TGG - chr6_4:18172544-18172563 Msa0980200:CDS 35.0%
CAATGAACTGGATGAACAAT+GGG - chr6_4:18172545-18172564 Msa0980200:CDS 35.0%
! TACCTTTTGTTGGGAATCAT+TGG - chr6_4:18172635-18172654 Msa0980200:CDS 35.0%
! ACCTTTTGTTGGGAATCATT+GGG - chr6_4:18172636-18172655 Msa0980200:CDS 35.0%
GGAAATTCGTTTGTTTGGAT+TGG + chr6_4:18172747-18172766 None:intergenic 35.0%
TCCAAACAAACGAATTTCCA+CGG - chr6_4:18172748-18172767 Msa0980200:CDS 35.0%
TGCGAGTTATAGATGTAGTT+TGG + chr6_4:18172782-18172801 None:intergenic 35.0%
GGCATAAAAACCACAAAAGT+TGG + chr6_4:18172807-18172826 None:intergenic 35.0%
GATCTGCAAGAAGGAAAAAA+TGG + chr6_4:18172920-18172939 None:intergenic 35.0%
ATTTGGTAAGATCTGCAAGA+AGG + chr6_4:18172929-18172948 None:intergenic 35.0%
CAGATCTTACCAAATGACTT+TGG - chr6_4:18172934-18172953 Msa0980200:intron 35.0%
CATTCTCATTTACGATTGTG+TGG - chr6_4:18172979-18172998 Msa0980200:intron 35.0%
GTGTTGCAAAGATTTACTTG+AGG + chr6_4:18172327-18172346 None:intergenic 35.0%
TGGAGTCCATTATGAATGAA+AGG + chr6_4:18172367-18172386 None:intergenic 35.0%
AGTGATGAAATAAGGTCTGT+TGG + chr6_4:18172387-18172406 None:intergenic 35.0%
! GCTTGATCCAATTTTCATTG+CGG - chr6_4:18172515-18172534 Msa0980200:CDS 35.0%
TCAATGAACTGGATGAACAA+TGG - chr6_4:18172544-18172563 Msa0980200:CDS 35.0%
CAATGAACTGGATGAACAAT+GGG - chr6_4:18172545-18172564 Msa0980200:CDS 35.0%
! TACCTTTTGTTGGGAATCAT+TGG - chr6_4:18172635-18172654 Msa0980200:CDS 35.0%
! ACCTTTTGTTGGGAATCATT+GGG - chr6_4:18172636-18172655 Msa0980200:CDS 35.0%
GGAAATTCGTTTGTTTGGAT+TGG + chr6_4:18172747-18172766 None:intergenic 35.0%
TCCAAACAAACGAATTTCCA+CGG - chr6_4:18172748-18172767 Msa0980200:CDS 35.0%
TGCGAGTTATAGATGTAGTT+TGG + chr6_4:18172782-18172801 None:intergenic 35.0%
GGCATAAAAACCACAAAAGT+TGG + chr6_4:18172807-18172826 None:intergenic 35.0%
GATCTGCAAGAAGGAAAAAA+TGG + chr6_4:18172920-18172939 None:intergenic 35.0%
ATTTGGTAAGATCTGCAAGA+AGG + chr6_4:18172929-18172948 None:intergenic 35.0%
CAGATCTTACCAAATGACTT+TGG - chr6_4:18172934-18172953 Msa0980200:intron 35.0%
CATTCTCATTTACGATTGTG+TGG - chr6_4:18172979-18172998 Msa0980200:intron 35.0%
TGCGGAATTCTTCAATGAAC+TGG - chr6_4:18172533-18172552 Msa0980200:CDS 40.0%
TCCCAATGATTCCCAACAAA+AGG + chr6_4:18172640-18172659 None:intergenic 40.0%
TTCCACATACACGTGTTTCA+TGG - chr6_4:18172720-18172739 Msa0980200:CDS 40.0%
ACCGTGGAAATTCGTTTGTT+TGG + chr6_4:18172752-18172771 None:intergenic 40.0%
TTGTTAAGATAGGACGTAGC+TGG - chr6_4:18173067-18173086 Msa0980200:CDS 40.0%
TGCGGAATTCTTCAATGAAC+TGG - chr6_4:18172533-18172552 Msa0980200:CDS 40.0%
TCCCAATGATTCCCAACAAA+AGG + chr6_4:18172640-18172659 None:intergenic 40.0%
TTCCACATACACGTGTTTCA+TGG - chr6_4:18172720-18172739 Msa0980200:CDS 40.0%
ACCGTGGAAATTCGTTTGTT+TGG + chr6_4:18172752-18172771 None:intergenic 40.0%
TTGTTAAGATAGGACGTAGC+TGG - chr6_4:18173067-18173086 Msa0980200:CDS 40.0%
GTAGTTTGGCTATGATACCG+TGG + chr6_4:18172768-18172787 None:intergenic 45.0%
! AACTCGCAACCCAACTTTTG+TGG - chr6_4:18172794-18172813 Msa0980200:CDS 45.0%
GTAGTTTGGCTATGATACCG+TGG + chr6_4:18172768-18172787 None:intergenic 45.0%
! AACTCGCAACCCAACTTTTG+TGG - chr6_4:18172794-18172813 Msa0980200:CDS 45.0%
GATCAAGCTCAGCACTCATC+TGG + chr6_4:18172503-18172522 None:intergenic 50.0%
GGCCATGAAACACGTGTATG+TGG + chr6_4:18172725-18172744 None:intergenic 50.0%
GATCAAGCTCAGCACTCATC+TGG + chr6_4:18172503-18172522 None:intergenic 50.0%
GGCCATGAAACACGTGTATG+TGG + chr6_4:18172725-18172744 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
chr6_4 gene 18172296 18173110 18172296 ID=Msa0980200;Name=Msa0980200
chr6_4 mRNA 18172296 18173110 18172296 ID=Msa0980200-mRNA-1;Parent=Msa0980200;Name=Msa0980200-mRNA-1;_AED=0.46;_eAED=0.46;_QI=0|1|0.5|1|1|1|2|160|191
chr6_4 exon 18172296 18172902 18172296 ID=Msa0980200-mRNA-1:exon:5632;Parent=Msa0980200-mRNA-1
chr6_4 exon 18172982 18173110 18172982 ID=Msa0980200-mRNA-1:exon:5631;Parent=Msa0980200-mRNA-1
chr6_4 CDS 18172982 18173110 18172982 ID=Msa0980200-mRNA-1:cds;Parent=Msa0980200-mRNA-1
chr6_4 CDS 18172456 18172902 18172456 ID=Msa0980200-mRNA-1:cds;Parent=Msa0980200-mRNA-1
chr6_4 three_prime_UTR 18172296 18172455 18172296 ID=Msa0980200-mRNA-1:three_prime_utr;Parent=Msa0980200-mRNA-1
Gene Sequence

>Msa0980200

ATGTATACTATATTTATATACACACATACCTCAAGTAAATCTTTGCAACACAATTTCATCTCCATCTTCCTTTCATTCATAATGGACTCCAACAGACCTTATTTCATCACTGTATGCAAAAAAAATCAGATGAGTGCTGAGCTTGATCCAATTTTCATTGCGGAATTCTTCAATGAACTGGATGAACAATGGGTCTTGATTGATGAAAATGAGAACCAACACATTGTTGTATTTAACCAAATTTTAACAATGCCTTATATTACCTTTTGTTGGGAATCATTGGGAATTTACTATGAAATCAATGATTATGGCCTAATATCTTTTACATATTTAGGTGAAAGTAAGTTCCACATACACGTGTTTCATGGCCCAATCCAAACAAACGAATTTCCACGGTATCATAGCCAAACTACATCTATAACTCGCAACCCAACTTTTGTGGTTTTTATGCCTAATATTGATGAATTTGGGACAAAATTGGTTAGTATTCACAACTGTCTTATATTTTTCTGGTCTTTATTTTTCTTACAATACAATCAATTCCATTTTTTCCTTCTTGCAGATCTTACCAAATGA

Protein sequence

>Msa0980200

MYTIFIYTHTSSKSLQHNFISIFLSFIMDSNRPYFITVCKKNQMSAELDPIFIAEFFNELDEQWVLIDENENQHIVVFNQILTMPYITFCWESLGIYYEINDYGLISFTYLGESKFHIHVFHGPIQTNEFPRYHSQTTSITRNPTFVVFMPNIDEFGTKLVSIHNCLIFFWSLFFLQYNQFHFFLLADLTK*