AlfalfaGEDB | Msa1458600

M. sativa cultivar zhongmu-4 / Msa1458600

KEGG: K14495
GO: GO:0000003 GO:0000151 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0007275 GO:0008150 GO:0009791 GO:0009845 GO:0010154 GO:0010162 GO:0010431 GO:0019005 GO:0021700 GO:0022414 GO:0022611 GO:0031461 GO:0032501 GO:0032502 GO:0032504 GO:0032991 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0048316 GO:0048608 GO:0048609 GO:0048731 GO:0048856 GO:0061458 GO:0071695 GO:0090351 GO:1902494 GO:1990234
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1458600	XP_013461725.1	94.944	178	5	1	1	178	1	174	3.14e-119	344

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1458600	sp\|Q9STX3\|GID2_ARATH	60.000	155	39	1	24	178	16	147	1.63e-60	187

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1458600	I3T3Z8	94.944	178	5	1	1	178	1	174	1.50e-119	344

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0014570	Msa1458600	0.817487	3.214743e-52	-8.615850e-47
Msa0044090	Msa1458600	0.809758	1.615341e-50	-8.615850e-47
Msa0058160	Msa1458600	0.810015	1.422065e-50	-8.615850e-47
Msa0061340	Msa1458600	0.804157	2.471826e-49	-8.615850e-47
Msa0089750	Msa1458600	0.831904	1.281233e-55	-8.615850e-47
Msa0106620	Msa1458600	0.802095	6.599765e-49	-8.615850e-47
Msa0106710	Msa1458600	0.807098	5.967283e-50	-8.615850e-47
Msa1355450	Msa1458600	0.810929	9.028214e-51	-8.615850e-47
Msa1369920	Msa1458600	-0.803903	2.792043e-49	-8.615850e-47
Msa1378940	Msa1458600	0.821495	3.916770e-53	-8.615850e-47
Msa1390680	Msa1458600	0.854138	1.502302e-61	-8.615850e-47
Msa1392890	Msa1458600	0.854213	1.429829e-61	-8.615850e-47
Msa1400930	Msa1458600	0.809837	1.553096e-50	-8.615850e-47
Msa1401270	Msa1458600	0.808292	3.327664e-50	-8.615850e-47
Msa1401460	Msa1458600	0.813440	2.556437e-51	-8.615850e-47
Msa1410550	Msa1458600	0.802729	4.886924e-49	-8.615850e-47
Msa1423780	Msa1458600	0.808555	2.923953e-50	-8.615850e-47
Msa1454530	Msa1458600	0.801890	7.272149e-49	-8.615850e-47
Msa1456230	Msa1458600	0.809658	1.696999e-50	-8.615850e-47
Msa1458600	Msa1465570	0.800474	1.417193e-48	-8.615850e-47
Msa0616550	Msa1458600	0.807213	5.641221e-50	-8.615850e-47
Msa0638350	Msa1458600	0.801243	9.872965e-49	-8.615850e-47
Msa0638360	Msa1458600	0.840965	6.328693e-58	-8.615850e-47
Msa0659210	Msa1458600	0.800914	1.152273e-48	-8.615850e-47
Msa0696850	Msa1458600	0.833971	3.924974e-56	-8.615850e-47
Msa0696860	Msa1458600	0.816182	6.308488e-52	-8.615850e-47
Msa0709270	Msa1458600	0.801956	7.049842e-49	-8.615850e-47
Msa0738240	Msa1458600	0.802336	5.886990e-49	-8.615850e-47
Msa0756380	Msa1458600	0.802323	5.924041e-49	-8.615850e-47
Msa0763890	Msa1458600	0.828643	8.019901e-55	-8.615850e-47
Msa0763920	Msa1458600	0.830439	2.934550e-55	-8.615850e-47
Msa0784480	Msa1458600	0.822038	2.932906e-53	-8.615850e-47
Msa0818060	Msa1458600	0.843425	1.412110e-58	-8.615850e-47
Msa0130720	Msa1458600	0.808103	3.649812e-50	-8.615850e-47
Msa0142460	Msa1458600	0.802672	5.019448e-49	-8.615850e-47
Msa0152400	Msa1458600	0.806683	7.301072e-50	-8.615850e-47
Msa0154440	Msa1458600	0.814676	1.364702e-51	-8.615850e-47
Msa0178740	Msa1458600	0.818326	2.078216e-52	-8.615850e-47
Msa0183760	Msa1458600	0.848831	4.769267e-60	-8.615850e-47
Msa0187830	Msa1458600	0.801186	1.014015e-48	-8.615850e-47
Msa0189040	Msa1458600	0.812753	3.618088e-51	-8.615850e-47
Msa0191890	Msa1458600	0.805517	1.284809e-49	-8.615850e-47
Msa0197650	Msa1458600	0.805506	1.291824e-49	-8.615850e-47
Msa0204840	Msa1458600	0.813479	2.506410e-51	-8.615850e-47
Msa0207040	Msa1458600	-0.813401	2.607607e-51	-8.615850e-47
Msa0220730	Msa1458600	0.822214	2.670408e-53	-8.615850e-47
Msa1232740	Msa1458600	0.803437	3.488396e-49	-8.615850e-47
Msa1243870	Msa1458600	0.816688	4.861862e-52	-8.615850e-47
Msa1246360	Msa1458600	0.806008	1.013131e-49	-8.615850e-47
Msa1262010	Msa1458600	0.811112	8.240082e-51	-8.615850e-47
Msa1271130	Msa1458600	0.819093	1.391845e-52	-8.615850e-47
Msa1277690	Msa1458600	0.809518	1.818377e-50	-8.615850e-47
Msa1288450	Msa1458600	0.801331	9.471110e-49	-8.615850e-47
Msa1313320	Msa1458600	0.804237	2.378248e-49	-8.615850e-47
Msa1322630	Msa1458600	0.806830	6.800189e-50	-8.615850e-47
Msa1323690	Msa1458600	0.806075	9.808940e-50	-8.615850e-47
Msa1330180	Msa1458600	0.813869	2.057788e-51	-8.615850e-47
Msa0367870	Msa1458600	0.814735	1.324142e-51	-8.615850e-47
Msa0382370	Msa1458600	0.847752	9.479900e-60	-8.615850e-47
Msa0388340	Msa1458600	0.824946	6.128459e-54	-8.615850e-47
Msa0389770	Msa1458600	0.801754	7.756780e-49	-8.615850e-47
Msa0395980	Msa1458600	0.808170	3.533243e-50	-8.615850e-47
Msa0430750	Msa1458600	0.820282	7.448763e-53	-8.615850e-47
Msa0432160	Msa1458600	0.855757	5.092216e-62	-8.615850e-47
Msa0450400	Msa1458600	-0.807102	5.956303e-50	-8.615850e-47
Msa0452710	Msa1458600	0.800813	1.208457e-48	-8.615850e-47
Msa0455760	Msa1458600	0.803794	2.940506e-49	-8.615850e-47
Msa0460590	Msa1458600	0.819013	1.451213e-52	-8.615850e-47
Msa0476680	Msa1458600	0.857764	1.306398e-62	-8.615850e-47
Msa0481970	Msa1458600	0.805138	1.542618e-49	-8.615850e-47
Msa0986510	Msa1458600	0.800243	1.579211e-48	-8.615850e-47
Msa1006010	Msa1458600	-0.831250	1.856396e-55	-8.615850e-47
Msa1032770	Msa1458600	0.833209	6.081189e-56	-8.615850e-47
Msa1034500	Msa1458600	0.855689	5.330470e-62	-8.615850e-47
Msa1036610	Msa1458600	0.815684	8.147858e-52	-8.615850e-47
Msa1079430	Msa1458600	0.804727	1.879720e-49	-8.615850e-47
Msa1089450	Msa1458600	0.802803	4.717069e-49	-8.615850e-47
Msa1100130	Msa1458600	-0.811420	7.063329e-51	-8.615850e-47
Msa0239810	Msa1458600	0.811166	8.020502e-51	-8.615850e-47
Msa0259230	Msa1458600	0.845801	3.236927e-59	-8.615850e-47
Msa0264830	Msa1458600	0.802482	5.494472e-49	-8.615850e-47
Msa0277420	Msa1458600	0.819578	1.079199e-52	-8.615850e-47
Msa0308460	Msa1458600	0.817594	3.041030e-52	-8.615850e-47
Msa0308630	Msa1458600	0.815125	1.085087e-51	-8.615850e-47
Msa0312530	Msa1458600	0.813964	1.960510e-51	-8.615850e-47
Msa0317970	Msa1458600	0.824437	8.077053e-54	-8.615850e-47
Msa0336300	Msa1458600	0.852820	3.592378e-61	-8.615850e-47
Msa0336320	Msa1458600	0.802672	5.019653e-49	-8.615850e-47
Msa0340340	Msa1458600	0.806774	6.986051e-50	-8.615850e-47
Msa0343850	Msa1458600	0.802206	6.261832e-49	-8.615850e-47
Msa1168550	Msa1458600	0.806427	8.271870e-50	-8.615850e-47
Msa1168590	Msa1458600	0.803097	4.102302e-49	-8.615850e-47
Msa1188710	Msa1458600	0.829407	5.238910e-55	-8.615850e-47
Msa1208580	Msa1458600	0.803359	3.619827e-49	-8.615850e-47
Msa0491000	Msa1458600	0.814399	1.571621e-51	-8.615850e-47
Msa0521010	Msa1458600	0.865553	5.432629e-65	-8.615850e-47
Msa0534400	Msa1458600	0.805078	1.588239e-49	-8.615850e-47
Msa0960390	Msa1458600	0.804808	1.808034e-49	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1458600	MtrunA17_Chr3g0134341	94.944	178	5	1	1	178	1	174	2.89e-123	344

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1458600	AT4G24210.1	60.000	155	39	1	24	178	16	147	1.66e-61	187
Msa1458600	AT5G48170.1	28.346	127	66	3	45	171	32	133	2.01e-11	59.7

Find 21 sgRNAs with CRISPR-Local

Find 31 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GTCTAAGCCTTTACCTCGTT+TGG	0.232824	tig0039679:-4738	Msa1458600:CDS
GACCTTATCACTTGTGGTAT+TGG	0.306662	tig0039679:+4758	Msa1458600:intergenic
CGATCTGTTGTTCTTGCTCT+TGG	0.347661	tig0039679:-4875	Msa1458600:CDS
CATGTTCTTCTTCATCATCA+TGG	0.373154	tig0039679:-4784	Msa1458600:CDS
TCTAAGCCTTTACCTCGTTT+GGG	0.384908	tig0039679:-4737	Msa1458600:CDS
CTGCACAAGATGAGAGACTT+TGG	0.406241	tig0039679:-4952	Msa1458600:CDS
ATGTTCTTCTTCATCATCAT+GGG	0.407721	tig0039679:-4783	Msa1458600:CDS
TCTGTTGTTCTTGCTCTTGG+TGG	0.449708	tig0039679:-4872	Msa1458600:CDS
TGCACAAGATGAGAGACTTT+GGG	0.489559	tig0039679:-4951	Msa1458600:CDS
GTCCAATACCACAAGTGATA+AGG	0.497370	tig0039679:-4760	Msa1458600:CDS
GGCTTAGACCTTATCACTTG+TGG	0.534034	tig0039679:+4752	Msa1458600:intergenic
GATGTTTGAGGCTTTGAAAG+AGG	0.571852	tig0039679:+4818	Msa1458600:intergenic
CAGTGGGCTAACACTGGCTG+TGG	0.598913	tig0039679:-4911	Msa1458600:CDS
TGTTCTTCTTCATCATCATG+GGG	0.610249	tig0039679:-4782	Msa1458600:CDS
ACAAAACAGTGGGCTAACAC+TGG	0.611464	tig0039679:-4917	Msa1458600:CDS
ACCTCGTTTGGGAAAAGACG+AGG	0.618915	tig0039679:-4726	Msa1458600:CDS
GCTGATCTGTACAAAACAGT+GGG	0.622628	tig0039679:-4927	Msa1458600:CDS
GGGGTTTGTGAGAAGAATCG+AGG	0.643342	tig0039679:-5076	Msa1458600:CDS
AGCTGATCTGTACAAAACAG+TGG	0.655674	tig0039679:-4928	Msa1458600:CDS
GGGTTTGTGAGAAGAATCGA+GGG	0.660517	tig0039679:-5075	Msa1458600:CDS
CTAGTTGTGTGAACAAACAA+TGG	0.693160	tig0039679:-4982	Msa1458600:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAAACAGTGAAAAAATTGAT+GGG	-	tig0039679:4728-4747	Msa1458600:CDS	20.0%
!	GAAAACAGTGAAAAAATTGA+TGG	-	tig0039679:4727-4746	Msa1458600:CDS	25.0%
!	AAACAGTGAAAAAATTGATG+GGG	-	tig0039679:4729-4748	Msa1458600:CDS	25.0%
	TGAAGACAGAAAAATGAAGA+GGG	-	tig0039679:4684-4703	Msa1458600:CDS	30.0%
	ATGTTCTTCTTCATCATCAT+GGG	-	tig0039679:5041-5060	Msa1458600:CDS	30.0%
!!	CTTCATTTTTCTGTCTTCAC+TGG	+	tig0039679:4683-4702	Msa1332550:intergenic	35.0%
	GTGAAGACAGAAAAATGAAG+AGG	-	tig0039679:4683-4702	Msa1458600:CDS	35.0%
	CTAGTTGTGTGAACAAACAA+TGG	-	tig0039679:4842-4861	Msa1458600:CDS	35.0%
!	GATGATGATGATGATGTTTG+AGG	+	tig0039679:5021-5040	Msa1458600:intergenic	35.0%
	CATGTTCTTCTTCATCATCA+TGG	-	tig0039679:5040-5059	Msa1458600:CDS	35.0%
	TGTTCTTCTTCATCATCATG+GGG	-	tig0039679:5042-5061	Msa1458600:CDS	35.0%
	CAGAAAAATGAAGAGGGTGA+AGG	-	tig0039679:4690-4709	Msa1458600:CDS	40.0%
	TGCACAAGATGAGAGACTTT+GGG	-	tig0039679:4873-4892	Msa1458600:CDS	40.0%
	AGCTGATCTGTACAAAACAG+TGG	-	tig0039679:4896-4915	Msa1458600:CDS	40.0%
	GCTGATCTGTACAAAACAGT+GGG	-	tig0039679:4897-4916	Msa1458600:CDS	40.0%
!!	GATGTTTGAGGCTTTGAAAG+AGG	+	tig0039679:5009-5028	Msa1458600:intergenic	40.0%
	GTCCAATACCACAAGTGATA+AGG	-	tig0039679:5064-5083	Msa1458600:CDS	40.0%
	GACCTTATCACTTGTGGTAT+TGG	+	tig0039679:5069-5088	Msa1458600:intergenic	40.0%
	TCTAAGCCTTTACCTCGTTT+GGG	-	tig0039679:5087-5106	Msa1458600:CDS	40.0%
!	TCTTTTCCCAAACGAGGTAA+AGG	+	tig0039679:5096-5115	Msa1458600:intergenic	40.0%
	GGGTTTGTGAGAAGAATCGA+GGG	-	tig0039679:4749-4768	Msa1458600:CDS	45.0%
	CTGCACAAGATGAGAGACTT+TGG	-	tig0039679:4872-4891	Msa1458600:CDS	45.0%
!!	ACAAAACAGTGGGCTAACAC+TGG	-	tig0039679:4907-4926	Msa1458600:CDS	45.0%
	CGATCTGTTGTTCTTGCTCT+TGG	-	tig0039679:4949-4968	Msa1458600:CDS	45.0%
	TCTGTTGTTCTTGCTCTTGG+TGG	-	tig0039679:4952-4971	Msa1458600:CDS	45.0%
	GGCTTAGACCTTATCACTTG+TGG	+	tig0039679:5075-5094	Msa1458600:intergenic	45.0%
	GTCTAAGCCTTTACCTCGTT+TGG	-	tig0039679:5086-5105	Msa1458600:CDS	45.0%
	GGGGTTTGTGAGAAGAATCG+AGG	-	tig0039679:4748-4767	Msa1458600:CDS	50.0%
	ACCTCGTTTGGGAAAAGACG+AGG	-	tig0039679:5098-5117	Msa1458600:CDS	50.0%
!	ACCTCGTCTTTTCCCAAACG+AGG	+	tig0039679:5102-5121	Msa1458600:intergenic	50.0%
!!	CAGTGGGCTAACACTGGCTG+TGG	-	tig0039679:4913-4932	Msa1458600:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
tig0039679	gene	4655	5191	4655	ID=Msa1458600;Name=Msa1458600
tig0039679	mRNA	4655	5191	4655	ID=Msa1458600-mRNA-1;Parent=Msa1458600;Name=Msa1458600-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|178
tig0039679	exon	4655	5191	4655	ID=Msa1458600-mRNA-1:exon:21844;Parent=Msa1458600-mRNA-1
tig0039679	CDS	4655	5191	4655	ID=Msa1458600-mRNA-1:cds;Parent=Msa1458600-mRNA-1

Gene Sequence

>Msa1458600

ATGAAGCGTTCACTTTCATCAGCAACCAGTGAAGACAGAAAAATGAAGAGGGTGAAGGAAGAAGAAAGCGATGAAAACAGTGAAAAAATTGATGGGGTTTGTGAGAAGAATCGAGGGCTAGCGAGTTTTGACGAGAATATGTTGTTTGAAGTTCTGAAACACGTTGACGCAAAGACTTTAGCTATGTCTAGTTGTGTGAACAAACAATGGCACAAAACTGCACAAGATGAGAGACTTTGGGAGCTGATCTGTACAAAACAGTGGGCTAACACTGGCTGTGGTGAACAACAGTTACGATCTGTTGTTCTTGCTCTTGGTGGATTTCGTCGTCTTCATTCTCTTTATCTTTTACCTCTTTCAAAGCCTCAAACATCATCATCATCATCATGTTCTTCTTCATCATCATGGGGTCCAATACCACAAGTGATAAGGTCTAAGCCTTTACCTCGTTTGGGAAAAGACGAGGTTCATCTCTCACTTTCACTTCTCTCTATTCGTTATTACGAGAAGATGAATTTCAACAACAGAAACCTATGA

Protein sequence

>Msa1458600

MKRSLSSATSEDRKMKRVKEEESDENSEKIDGVCEKNRGLASFDENMLFEVLKHVDAKTLAMSSCVNKQWHKTAQDERLWELICTKQWANTGCGEQQLRSVVLALGGFRRLHSLYLLPLSKPQTSSSSSCSSSSSWGPIPQVIRSKPLPRLGKDEVHLSLSLLSIRYYEKMNFNNRNL*