AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0271860

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0271860 MtrunA17_Chr2g0279611 94.416 197 11 0 1 197 1 197 1.53e-141 392
Msa0271860 MtrunA17_Chr4g0049871 68.208 173 54 1 3 175 2 173 6.25e-86 251
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0271860 AT2G14110.1 68.132 182 58 0 6 187 4 185 3.41e-94 273
Msa0271860 AT2G14110.2 69.925 133 40 0 4 136 2 134 4.52e-70 210

Find 48 sgRNAs with CRISPR-Local

Find 256 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTACATATAGGTCTCTAAAA+TGG 0.207038 2_3:+3378296 Msa0271860:intron
AATGGGAGTGACTAGCATTT+TGG 0.255669 2_3:+3378314 Msa0271860:CDS
AGATCGAAAACAACGAGTTT+TGG 0.286511 2_3:-3376698 None:intergenic
GTTGATAATGGGGTAAATCT+TGG 0.322461 2_3:+3378336 Msa0271860:CDS
CTAGTATGATTATAGAAAAC+AGG 0.336024 2_3:-3378695 None:intergenic
ATCTGGCTTCTTAGAATAAT+CGG 0.355976 2_3:-3378433 None:intergenic
TACTTGGACACTGAATTTGA+TGG 0.360359 2_3:-3378471 None:intergenic
TGCTTCGTTCTTCGTTGCTC+AGG 0.372516 2_3:+3376898 Msa0271860:CDS
GGGGCGGGGTTGGAAGTATC+TGG 0.373055 2_3:-3378450 None:intergenic
CAGCGAATTCATTCTACTAC+TGG 0.376753 2_3:+3377043 Msa0271860:CDS
GCTCAATCAAATTAATCGTT+GGG 0.398355 2_3:+3376584 Msa0271860:five_prime_UTR
TGAATTTGATGGGGCGGGGT+TGG 0.401193 2_3:-3378460 None:intergenic
CACAGAAGAACAAGCAGAAA+TGG 0.408899 2_3:+3378406 Msa0271860:CDS
CGCTCAATCAAATTAATCGT+TGG 0.422131 2_3:+3376583 Msa0271860:five_prime_UTR
CACGACAGAGGTAACATGTT+TGG 0.437740 2_3:-3378607 None:intergenic
GAGCAGAGAGAAAGCGAAAT+GGG 0.444666 2_3:+3376614 Msa0271860:exon
AGAGCAGAGAGAAAGCGAAA+TGG 0.444737 2_3:+3376613 Msa0271860:exon
TACATATAGGTCTCTAAAAT+GGG 0.446794 2_3:+3378297 Msa0271860:intron
TCACGCTATGGGAATCTTGT+TGG 0.449058 2_3:+3376787 Msa0271860:CDS
TTCAGGAAATATATTCGAGT+TGG 0.451128 2_3:+3376999 Msa0271860:intron
TCGATCTCGATTACACTCTC+TGG 0.456392 2_3:+3376714 Msa0271860:CDS
TTCTCTATATCATCACGCTA+TGG 0.459033 2_3:+3376775 Msa0271860:CDS
GACACTGAATTTGATGGGGC+GGG 0.459346 2_3:-3378465 None:intergenic
ACTTGGACACTGAATTTGAT+GGG 0.473301 2_3:-3378470 None:intergenic
TTACCTCTGTCGTGTATACT+TGG 0.484966 2_3:+3378616 Msa0271860:three_prime_UTR
AAGCGAAATGGGAGAGTCTA+CGG 0.501518 2_3:+3376625 Msa0271860:exon
TCTCTATATCATCACGCTAT+GGG 0.504273 2_3:+3376776 Msa0271860:CDS
ATTGAGACTACAATTCAAGT+AGG 0.515093 2_3:-3378570 None:intergenic
GATCGACACTCGCAGTAGAA+AGG 0.521923 2_3:-3376737 None:intergenic
TAGAGAAGGAGTTTCGCGCT+TGG 0.523146 2_3:-3376759 None:intergenic
CTGTGTAAGACCTTTAGATA+AGG 0.524076 2_3:-3378361 None:intergenic
GCTTTAGCAATATCCGCAGT+TGG 0.525120 2_3:-3376854 None:intergenic
TTCGAGTTGGACTCATAAAA+CGG 0.526393 2_3:+3377012 Msa0271860:CDS
AATCTTGGAGCCTTATCTAA+AGG 0.528346 2_3:+3378351 Msa0271860:CDS
CTCTAAATACATGAATATCA+AGG 0.549756 2_3:-3378671 None:intergenic
CTCAATCAAATTAATCGTTG+GGG 0.565833 2_3:+3376585 Msa0271860:five_prime_UTR
ACACTGAATTTGATGGGGCG+GGG 0.585099 2_3:-3378464 None:intergenic
AGTCTCAATCAAATTACTGA+AGG 0.588172 2_3:+3378584 Msa0271860:three_prime_UTR
GGACACTGAATTTGATGGGG+CGG 0.589207 2_3:-3378466 None:intergenic
AATGGGAGAGTCTACGGAGA+AGG 0.592110 2_3:+3376631 Msa0271860:exon
CTTGGACACTGAATTTGATG+GGG 0.607262 2_3:-3378469 None:intergenic
CGCTTCTCGATCTCCAACTG+CGG 0.610594 2_3:+3376841 Msa0271860:CDS
ATAGCGTGATGATATAGAGA+AGG 0.642803 2_3:-3376773 None:intergenic
TAGTATGATTATAGAAAACA+GGG 0.650221 2_3:-3378694 None:intergenic
TTGTTGGCGCTTAAACACAA+AGG 0.670200 2_3:+3376803 Msa0271860:CDS
TCAATCAAATTAATCGTTGG+GGG 0.692972 2_3:+3376586 Msa0271860:five_prime_UTR
TGAGAATAGAAACATCCAAG+CGG 0.730982 2_3:+3377099 Msa0271860:CDS
ATGCCAAGTATACACGACAG+AGG 0.736619 2_3:-3378619 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TGTCATTTTTTTTTTTTTTT+TGG - chr2_3:3377248-3377267 None:intergenic 10.0%
!!! TTTTGTATTTAAATTAGTTA+GGG + chr2_3:3377342-3377361 Msa0271860:intron 10.0%
!!! TGTCATTTTTTTTTTTTTTT+TGG - chr2_3:3377248-3377267 None:intergenic 10.0%
!!! TTTTGTATTTAAATTAGTTA+GGG + chr2_3:3377342-3377361 Msa0271860:intron 10.0%
!!! TAAAGCTTTTCTTAATAAAT+TGG + chr2_3:3376871-3376890 Msa0271860:CDS 15.0%
!!! AAAGCTTTTCTTAATAAATT+GGG + chr2_3:3376872-3376891 Msa0271860:CDS 15.0%
!!! GTTTTGTATTTAAATTAGTT+AGG + chr2_3:3377341-3377360 Msa0271860:intron 15.0%
!! TTTGTATTTAAATTAGTTAG+GGG + chr2_3:3377343-3377362 Msa0271860:intron 15.0%
!! AAAAAAAAAATTGATTATGC+AGG + chr2_3:3377492-3377511 Msa0271860:intron 15.0%
!!! TAAAGCTTTTCTTAATAAAT+TGG + chr2_3:3376871-3376890 Msa0271860:CDS 15.0%
!!! AAAGCTTTTCTTAATAAATT+GGG + chr2_3:3376872-3376891 Msa0271860:CDS 15.0%
!!! GTTTTGTATTTAAATTAGTT+AGG + chr2_3:3377341-3377360 Msa0271860:intron 15.0%
!! TTTGTATTTAAATTAGTTAG+GGG + chr2_3:3377343-3377362 Msa0271860:intron 15.0%
!! AAAAAAAAAATTGATTATGC+AGG + chr2_3:3377492-3377511 Msa0271860:intron 15.0%
!! TGAACAAAACAACAAAAATT+AGG - chr2_3:3376983-3377002 None:intergenic 20.0%
!!! TAATTTTTGTTGTTTTGTTC+AGG + chr2_3:3376982-3377001 Msa0271860:intron 20.0%
!!! AAGATTAGTTAAAATCACAT+TGG - chr2_3:3377205-3377224 None:intergenic 20.0%
!!! ATGTGATTTTAACTAATCTT+TGG + chr2_3:3377205-3377224 Msa0271860:intron 20.0%
!!! TTTTTTTTTTTTTTGGCCTT+GGG - chr2_3:3377241-3377260 None:intergenic 20.0%
!!! TTTTTTTTTTTTTTTGGCCT+TGG - chr2_3:3377242-3377261 None:intergenic 20.0%
!! AAAAAAAAAAAAATGACACG+TGG + chr2_3:3377250-3377269 Msa0271860:intron 20.0%
!! TTGTATTTAAATTAGTTAGG+GGG + chr2_3:3377344-3377363 Msa0271860:intron 20.0%
!! AATGCTAAAAACAATTATAG+AGG - chr2_3:3377578-3377597 None:intergenic 20.0%
!!! AATCAATTTATAGCTTGTTT+TGG + chr2_3:3377680-3377699 Msa0271860:intron 20.0%
!!! TTACAATTCTAGTTTTAAGT+TGG + chr2_3:3377861-3377880 Msa0271860:intron 20.0%
!! AGTAAATTTGACAAAATCAT+GGG - chr2_3:3378174-3378193 None:intergenic 20.0%
!! AAGTAAATTTGACAAAATCA+TGG - chr2_3:3378175-3378194 None:intergenic 20.0%
!! TTATGTAAAAGTTACTTAGT+TGG - chr2_3:3378263-3378282 None:intergenic 20.0%
!! TTGTTTATATCGTTACATAT+AGG + chr2_3:3378284-3378303 Msa0271860:intron 20.0%
!!! AATCCAAAGAATTTTTTACT+TGG - chr2_3:3378490-3378509 None:intergenic 20.0%
!! TAGTATGATTATAGAAAACA+GGG - chr2_3:3378697-3378716 None:intergenic 20.0%
!! TGAACAAAACAACAAAAATT+AGG - chr2_3:3376983-3377002 None:intergenic 20.0%
!!! TAATTTTTGTTGTTTTGTTC+AGG + chr2_3:3376982-3377001 Msa0271860:intron 20.0%
!!! AAGATTAGTTAAAATCACAT+TGG - chr2_3:3377205-3377224 None:intergenic 20.0%
!!! ATGTGATTTTAACTAATCTT+TGG + chr2_3:3377205-3377224 Msa0271860:intron 20.0%
!!! TTTTTTTTTTTTTTGGCCTT+GGG - chr2_3:3377241-3377260 None:intergenic 20.0%
!!! TTTTTTTTTTTTTTTGGCCT+TGG - chr2_3:3377242-3377261 None:intergenic 20.0%
!! AAAAAAAAAAAAATGACACG+TGG + chr2_3:3377250-3377269 Msa0271860:intron 20.0%
!! TTGTATTTAAATTAGTTAGG+GGG + chr2_3:3377344-3377363 Msa0271860:intron 20.0%
!! AATGCTAAAAACAATTATAG+AGG - chr2_3:3377578-3377597 None:intergenic 20.0%
!!! AATCAATTTATAGCTTGTTT+TGG + chr2_3:3377680-3377699 Msa0271860:intron 20.0%
!!! TTACAATTCTAGTTTTAAGT+TGG + chr2_3:3377861-3377880 Msa0271860:intron 20.0%
!! AGTAAATTTGACAAAATCAT+GGG - chr2_3:3378174-3378193 None:intergenic 20.0%
!! AAGTAAATTTGACAAAATCA+TGG - chr2_3:3378175-3378194 None:intergenic 20.0%
!! TTATGTAAAAGTTACTTAGT+TGG - chr2_3:3378263-3378282 None:intergenic 20.0%
!! TTGTTTATATCGTTACATAT+AGG + chr2_3:3378284-3378303 Msa0271860:intron 20.0%
!!! AATCCAAAGAATTTTTTACT+TGG - chr2_3:3378490-3378509 None:intergenic 20.0%
!! TAGTATGATTATAGAAAACA+GGG - chr2_3:3378697-3378716 None:intergenic 20.0%
!!! TTTTTTTTTTTTTGGCCTTG+GGG - chr2_3:3377240-3377259 None:intergenic 25.0%
!!! AAACTCTAGAAGTAGTTTTT+AGG - chr2_3:3377418-3377437 None:intergenic 25.0%
! AGAATAAATTGATTATGCAG+TGG + chr2_3:3377462-3377481 Msa0271860:intron 25.0%
!!! TTTAGCTTGAGATTAGAATA+TGG + chr2_3:3377544-3377563 Msa0271860:intron 25.0%
!! TTGTCTAAAAAATGAGTTGA+AGG - chr2_3:3377660-3377679 None:intergenic 25.0%
! TGAGTTCAACAATAAATTTG+AGG + chr2_3:3377812-3377831 Msa0271860:intron 25.0%
! GAGTTCAACAATAAATTTGA+GGG + chr2_3:3377813-3377832 Msa0271860:intron 25.0%
! CAACATAAGCTTATGAATAA+AGG - chr2_3:3378066-3378085 None:intergenic 25.0%
! TAAATTTGACAAAATCATGG+GGG - chr2_3:3378172-3378191 None:intergenic 25.0%
! GTAAATTTGACAAAATCATG+GGG - chr2_3:3378173-3378192 None:intergenic 25.0%
!! TATATCATGTGCATCATTTT+TGG - chr2_3:3378203-3378222 None:intergenic 25.0%
! AGTTACTTAGTTGGAAAAAT+GGG - chr2_3:3378254-3378273 None:intergenic 25.0%
! AAGTTACTTAGTTGGAAAAA+TGG - chr2_3:3378255-3378274 None:intergenic 25.0%
!! TTACATATAGGTCTCTAAAA+TGG + chr2_3:3378296-3378315 Msa0271860:intron 25.0%
!!! TACATATAGGTCTCTAAAAT+GGG + chr2_3:3378297-3378316 Msa0271860:intron 25.0%
! TGTCCAAGTAAAAAATTCTT+TGG + chr2_3:3378484-3378503 Msa0271860:exon 25.0%
! CTCTAAATACATGAATATCA+AGG - chr2_3:3378674-3378693 None:intergenic 25.0%
! CTAGTATGATTATAGAAAAC+AGG - chr2_3:3378698-3378717 None:intergenic 25.0%
!!! TTTTTTTTTTTTTGGCCTTG+GGG - chr2_3:3377240-3377259 None:intergenic 25.0%
!!! AAACTCTAGAAGTAGTTTTT+AGG - chr2_3:3377418-3377437 None:intergenic 25.0%
! AGAATAAATTGATTATGCAG+TGG + chr2_3:3377462-3377481 Msa0271860:intron 25.0%
!!! TTTAGCTTGAGATTAGAATA+TGG + chr2_3:3377544-3377563 Msa0271860:intron 25.0%
!! TTGTCTAAAAAATGAGTTGA+AGG - chr2_3:3377660-3377679 None:intergenic 25.0%
! TGAGTTCAACAATAAATTTG+AGG + chr2_3:3377812-3377831 Msa0271860:intron 25.0%
! GAGTTCAACAATAAATTTGA+GGG + chr2_3:3377813-3377832 Msa0271860:intron 25.0%
! CAACATAAGCTTATGAATAA+AGG - chr2_3:3378066-3378085 None:intergenic 25.0%
! TAAATTTGACAAAATCATGG+GGG - chr2_3:3378172-3378191 None:intergenic 25.0%
! GTAAATTTGACAAAATCATG+GGG - chr2_3:3378173-3378192 None:intergenic 25.0%
!! TATATCATGTGCATCATTTT+TGG - chr2_3:3378203-3378222 None:intergenic 25.0%
! AGTTACTTAGTTGGAAAAAT+GGG - chr2_3:3378254-3378273 None:intergenic 25.0%
! AAGTTACTTAGTTGGAAAAA+TGG - chr2_3:3378255-3378274 None:intergenic 25.0%
!! TTACATATAGGTCTCTAAAA+TGG + chr2_3:3378296-3378315 Msa0271860:intron 25.0%
!!! TACATATAGGTCTCTAAAAT+GGG + chr2_3:3378297-3378316 Msa0271860:intron 25.0%
! TGTCCAAGTAAAAAATTCTT+TGG + chr2_3:3378484-3378503 Msa0271860:exon 25.0%
! CTCTAAATACATGAATATCA+AGG - chr2_3:3378674-3378693 None:intergenic 25.0%
! CTAGTATGATTATAGAAAAC+AGG - chr2_3:3378698-3378717 None:intergenic 25.0%
GCTCAATCAAATTAATCGTT+GGG + chr2_3:3376584-3376603 Msa0271860:five_prime_UTR 30.0%
CTCAATCAAATTAATCGTTG+GGG + chr2_3:3376585-3376604 Msa0271860:five_prime_UTR 30.0%
TCAATCAAATTAATCGTTGG+GGG + chr2_3:3376586-3376605 Msa0271860:five_prime_UTR 30.0%
TTCAGGAAATATATTCGAGT+TGG + chr2_3:3376999-3377018 Msa0271860:intron 30.0%
! AAAAAGAGCATAGAGCTAAA+CGG - chr2_3:3377073-3377092 None:intergenic 30.0%
GATTAATGATTATTACCGCT+TGG - chr2_3:3377117-3377136 None:intergenic 30.0%
ATAGTGTATGACTGTATCTT+TGG + chr2_3:3377150-3377169 Msa0271860:intron 30.0%
! TAACTAATCTTTGGCAGTTT+TGG + chr2_3:3377214-3377233 Msa0271860:intron 30.0%
! TATTGAATCTTTGGCAGTTT+TGG - chr2_3:3377310-3377329 None:intergenic 30.0%
TGCCAAAGATTCAATACTTA+GGG + chr2_3:3377314-3377333 Msa0271860:intron 30.0%
! TTTAGGCTTAATTGCACTTT+TGG - chr2_3:3377401-3377420 None:intergenic 30.0%
GGAATTGATTATGACATGTT+TGG - chr2_3:3377915-3377934 None:intergenic 30.0%
GTAAGTTGTTTATCCAAACA+CGG + chr2_3:3378097-3378116 Msa0271860:intron 30.0%
ATTATGAATAAGACCGTGTT+TGG - chr2_3:3378113-3378132 None:intergenic 30.0%
GTTACTTAGTTGGAAAAATG+GGG - chr2_3:3378253-3378272 None:intergenic 30.0%
!!! ACTAGCATTTTGGTTGATAA+TGG + chr2_3:3378324-3378343 Msa0271860:CDS 30.0%
!!! CTAGCATTTTGGTTGATAAT+GGG + chr2_3:3378325-3378344 Msa0271860:CDS 30.0%
!!! TAGCATTTTGGTTGATAATG+GGG + chr2_3:3378326-3378345 Msa0271860:CDS 30.0%
! ATCTGGCTTCTTAGAATAAT+CGG - chr2_3:3378436-3378455 None:intergenic 30.0%
ATTGAGACTACAATTCAAGT+AGG - chr2_3:3378573-3378592 None:intergenic 30.0%
AGTCTCAATCAAATTACTGA+AGG + chr2_3:3378584-3378603 Msa0271860:three_prime_UTR 30.0%
GCTCAATCAAATTAATCGTT+GGG + chr2_3:3376584-3376603 Msa0271860:five_prime_UTR 30.0%
CTCAATCAAATTAATCGTTG+GGG + chr2_3:3376585-3376604 Msa0271860:five_prime_UTR 30.0%
TCAATCAAATTAATCGTTGG+GGG + chr2_3:3376586-3376605 Msa0271860:five_prime_UTR 30.0%
TTCAGGAAATATATTCGAGT+TGG + chr2_3:3376999-3377018 Msa0271860:intron 30.0%
! AAAAAGAGCATAGAGCTAAA+CGG - chr2_3:3377073-3377092 None:intergenic 30.0%
GATTAATGATTATTACCGCT+TGG - chr2_3:3377117-3377136 None:intergenic 30.0%
ATAGTGTATGACTGTATCTT+TGG + chr2_3:3377150-3377169 Msa0271860:intron 30.0%
! TAACTAATCTTTGGCAGTTT+TGG + chr2_3:3377214-3377233 Msa0271860:intron 30.0%
! TATTGAATCTTTGGCAGTTT+TGG - chr2_3:3377310-3377329 None:intergenic 30.0%
TGCCAAAGATTCAATACTTA+GGG + chr2_3:3377314-3377333 Msa0271860:intron 30.0%
! TTTAGGCTTAATTGCACTTT+TGG - chr2_3:3377401-3377420 None:intergenic 30.0%
GGAATTGATTATGACATGTT+TGG - chr2_3:3377915-3377934 None:intergenic 30.0%
GTAAGTTGTTTATCCAAACA+CGG + chr2_3:3378097-3378116 Msa0271860:intron 30.0%
ATTATGAATAAGACCGTGTT+TGG - chr2_3:3378113-3378132 None:intergenic 30.0%
GTTACTTAGTTGGAAAAATG+GGG - chr2_3:3378253-3378272 None:intergenic 30.0%
!!! ACTAGCATTTTGGTTGATAA+TGG + chr2_3:3378324-3378343 Msa0271860:CDS 30.0%
!!! CTAGCATTTTGGTTGATAAT+GGG + chr2_3:3378325-3378344 Msa0271860:CDS 30.0%
!!! TAGCATTTTGGTTGATAATG+GGG + chr2_3:3378326-3378345 Msa0271860:CDS 30.0%
! ATCTGGCTTCTTAGAATAAT+CGG - chr2_3:3378436-3378455 None:intergenic 30.0%
ATTGAGACTACAATTCAAGT+AGG - chr2_3:3378573-3378592 None:intergenic 30.0%
AGTCTCAATCAAATTACTGA+AGG + chr2_3:3378584-3378603 Msa0271860:three_prime_UTR 30.0%
CGCTCAATCAAATTAATCGT+TGG + chr2_3:3376583-3376602 Msa0271860:five_prime_UTR 35.0%
! AGATCGAAAACAACGAGTTT+TGG - chr2_3:3376701-3376720 None:intergenic 35.0%
ATAGCGTGATGATATAGAGA+AGG - chr2_3:3376776-3376795 None:intergenic 35.0%
TTCTCTATATCATCACGCTA+TGG + chr2_3:3376775-3376794 Msa0271860:CDS 35.0%
TCTCTATATCATCACGCTAT+GGG + chr2_3:3376776-3376795 Msa0271860:CDS 35.0%
!! TTCGAGTTGGACTCATAAAA+CGG + chr2_3:3377012-3377031 Msa0271860:CDS 35.0%
TGAGAATAGAAACATCCAAG+CGG + chr2_3:3377099-3377118 Msa0271860:CDS 35.0%
TGACTGTATCTTTGGATTGA+TGG + chr2_3:3377158-3377177 Msa0271860:intron 35.0%
CTGCCAAAGATTCAATACTT+AGG + chr2_3:3377313-3377332 Msa0271860:intron 35.0%
GCCAAAGATTCAATACTTAG+GGG + chr2_3:3377315-3377334 Msa0271860:intron 35.0%
CCAAAGATTCAATACTTAGG+GGG + chr2_3:3377316-3377335 Msa0271860:intron 35.0%
CTCTATCTTTCCAAAACTTG+CGG - chr2_3:3377376-3377395 None:intergenic 35.0%
!!! GCAAGTTTTGGAAAGATAGA+GGG + chr2_3:3377375-3377394 Msa0271860:intron 35.0%
!! GGATTGACTTGATGTTTGTT+TGG + chr2_3:3377701-3377720 Msa0271860:intron 35.0%
!! TTCGACTAGAATTGAGTTGA+AGG + chr2_3:3377753-3377772 Msa0271860:intron 35.0%
! CAAAATTGATTCTAGAGGTG+TGG - chr2_3:3377936-3377955 None:intergenic 35.0%
!!! CACACCTCTAGAATCAATTT+TGG + chr2_3:3377934-3377953 Msa0271860:intron 35.0%
ACCAATTTGAGCTTATCTTC+TGG + chr2_3:3377973-3377992 Msa0271860:intron 35.0%
TCCAGAAGATAAGCTCAAAT+TGG - chr2_3:3377977-3377996 None:intergenic 35.0%
!! GTTGATAATGGGGTAAATCT+TGG + chr2_3:3378336-3378355 Msa0271860:CDS 35.0%
! AATCTTGGAGCCTTATCTAA+AGG + chr2_3:3378351-3378370 Msa0271860:CDS 35.0%
CTGTGTAAGACCTTTAGATA+AGG - chr2_3:3378364-3378383 None:intergenic 35.0%
! TTACACAGTTTTCTCGAAAC+TGG + chr2_3:3378376-3378395 Msa0271860:CDS 35.0%
ACTTGGACACTGAATTTGAT+GGG - chr2_3:3378473-3378492 None:intergenic 35.0%
TACTTGGACACTGAATTTGA+TGG - chr2_3:3378474-3378493 None:intergenic 35.0%
CGCTCAATCAAATTAATCGT+TGG + chr2_3:3376583-3376602 Msa0271860:five_prime_UTR 35.0%
! AGATCGAAAACAACGAGTTT+TGG - chr2_3:3376701-3376720 None:intergenic 35.0%
ATAGCGTGATGATATAGAGA+AGG - chr2_3:3376776-3376795 None:intergenic 35.0%
TTCTCTATATCATCACGCTA+TGG + chr2_3:3376775-3376794 Msa0271860:CDS 35.0%
TCTCTATATCATCACGCTAT+GGG + chr2_3:3376776-3376795 Msa0271860:CDS 35.0%
!! TTCGAGTTGGACTCATAAAA+CGG + chr2_3:3377012-3377031 Msa0271860:CDS 35.0%
TGAGAATAGAAACATCCAAG+CGG + chr2_3:3377099-3377118 Msa0271860:CDS 35.0%
TGACTGTATCTTTGGATTGA+TGG + chr2_3:3377158-3377177 Msa0271860:intron 35.0%
CTGCCAAAGATTCAATACTT+AGG + chr2_3:3377313-3377332 Msa0271860:intron 35.0%
GCCAAAGATTCAATACTTAG+GGG + chr2_3:3377315-3377334 Msa0271860:intron 35.0%
CCAAAGATTCAATACTTAGG+GGG + chr2_3:3377316-3377335 Msa0271860:intron 35.0%
CTCTATCTTTCCAAAACTTG+CGG - chr2_3:3377376-3377395 None:intergenic 35.0%
!!! GCAAGTTTTGGAAAGATAGA+GGG + chr2_3:3377375-3377394 Msa0271860:intron 35.0%
!! GGATTGACTTGATGTTTGTT+TGG + chr2_3:3377701-3377720 Msa0271860:intron 35.0%
!! TTCGACTAGAATTGAGTTGA+AGG + chr2_3:3377753-3377772 Msa0271860:intron 35.0%
! CAAAATTGATTCTAGAGGTG+TGG - chr2_3:3377936-3377955 None:intergenic 35.0%
!!! CACACCTCTAGAATCAATTT+TGG + chr2_3:3377934-3377953 Msa0271860:intron 35.0%
ACCAATTTGAGCTTATCTTC+TGG + chr2_3:3377973-3377992 Msa0271860:intron 35.0%
TCCAGAAGATAAGCTCAAAT+TGG - chr2_3:3377977-3377996 None:intergenic 35.0%
!! GTTGATAATGGGGTAAATCT+TGG + chr2_3:3378336-3378355 Msa0271860:CDS 35.0%
! AATCTTGGAGCCTTATCTAA+AGG + chr2_3:3378351-3378370 Msa0271860:CDS 35.0%
CTGTGTAAGACCTTTAGATA+AGG - chr2_3:3378364-3378383 None:intergenic 35.0%
! TTACACAGTTTTCTCGAAAC+TGG + chr2_3:3378376-3378395 Msa0271860:CDS 35.0%
ACTTGGACACTGAATTTGAT+GGG - chr2_3:3378473-3378492 None:intergenic 35.0%
TACTTGGACACTGAATTTGA+TGG - chr2_3:3378474-3378493 None:intergenic 35.0%
TTGTTGGCGCTTAAACACAA+AGG + chr2_3:3376803-3376822 Msa0271860:CDS 40.0%
CAGCGAATTCATTCTACTAC+TGG + chr2_3:3377043-3377062 Msa0271860:CDS 40.0%
!! CCCCCTAAGTATTGAATCTT+TGG - chr2_3:3377319-3377338 None:intergenic 40.0%
!!! CGCAAGTTTTGGAAAGATAG+AGG + chr2_3:3377374-3377393 Msa0271860:intron 40.0%
! GGAGCCAAAATTGATTCTAG+AGG - chr2_3:3377941-3377960 None:intergenic 40.0%
!!! AATTTTGGCTCCTCCAAAAG+TGG + chr2_3:3377949-3377968 Msa0271860:intron 40.0%
!! CAAATTGGTTCCACTTTTGG+AGG - chr2_3:3377962-3377981 None:intergenic 40.0%
!! GCTCAAATTGGTTCCACTTT+TGG - chr2_3:3377965-3377984 None:intergenic 40.0%
! AATGGGAGTGACTAGCATTT+TGG + chr2_3:3378314-3378333 Msa0271860:CDS 40.0%
CACAGAAGAACAAGCAGAAA+TGG + chr2_3:3378406-3378425 Msa0271860:CDS 40.0%
CTTGGACACTGAATTTGATG+GGG - chr2_3:3378472-3378491 None:intergenic 40.0%
TTACCTCTGTCGTGTATACT+TGG + chr2_3:3378616-3378635 Msa0271860:three_prime_UTR 40.0%
TTGTTGGCGCTTAAACACAA+AGG + chr2_3:3376803-3376822 Msa0271860:CDS 40.0%
CAGCGAATTCATTCTACTAC+TGG + chr2_3:3377043-3377062 Msa0271860:CDS 40.0%
!! CCCCCTAAGTATTGAATCTT+TGG - chr2_3:3377319-3377338 None:intergenic 40.0%
!!! CGCAAGTTTTGGAAAGATAG+AGG + chr2_3:3377374-3377393 Msa0271860:intron 40.0%
! GGAGCCAAAATTGATTCTAG+AGG - chr2_3:3377941-3377960 None:intergenic 40.0%
!!! AATTTTGGCTCCTCCAAAAG+TGG + chr2_3:3377949-3377968 Msa0271860:intron 40.0%
!! CAAATTGGTTCCACTTTTGG+AGG - chr2_3:3377962-3377981 None:intergenic 40.0%
!! GCTCAAATTGGTTCCACTTT+TGG - chr2_3:3377965-3377984 None:intergenic 40.0%
! AATGGGAGTGACTAGCATTT+TGG + chr2_3:3378314-3378333 Msa0271860:CDS 40.0%
CACAGAAGAACAAGCAGAAA+TGG + chr2_3:3378406-3378425 Msa0271860:CDS 40.0%
CTTGGACACTGAATTTGATG+GGG - chr2_3:3378472-3378491 None:intergenic 40.0%
TTACCTCTGTCGTGTATACT+TGG + chr2_3:3378616-3378635 Msa0271860:three_prime_UTR 40.0%
AGAGCAGAGAGAAAGCGAAA+TGG + chr2_3:3376613-3376632 Msa0271860:exon 45.0%
GAGCAGAGAGAAAGCGAAAT+GGG + chr2_3:3376614-3376633 Msa0271860:exon 45.0%
AAGCGAAATGGGAGAGTCTA+CGG + chr2_3:3376625-3376644 Msa0271860:exon 45.0%
TCGATCTCGATTACACTCTC+TGG + chr2_3:3376714-3376733 Msa0271860:CDS 45.0%
TCACGCTATGGGAATCTTGT+TGG + chr2_3:3376787-3376806 Msa0271860:CDS 45.0%
GCTTTAGCAATATCCGCAGT+TGG - chr2_3:3376857-3376876 None:intergenic 45.0%
CACGACAGAGGTAACATGTT+TGG - chr2_3:3378610-3378629 None:intergenic 45.0%
ATGCCAAGTATACACGACAG+AGG - chr2_3:3378622-3378641 None:intergenic 45.0%
AGAGCAGAGAGAAAGCGAAA+TGG + chr2_3:3376613-3376632 Msa0271860:exon 45.0%
GAGCAGAGAGAAAGCGAAAT+GGG + chr2_3:3376614-3376633 Msa0271860:exon 45.0%
AAGCGAAATGGGAGAGTCTA+CGG + chr2_3:3376625-3376644 Msa0271860:exon 45.0%
TCGATCTCGATTACACTCTC+TGG + chr2_3:3376714-3376733 Msa0271860:CDS 45.0%
TCACGCTATGGGAATCTTGT+TGG + chr2_3:3376787-3376806 Msa0271860:CDS 45.0%
GCTTTAGCAATATCCGCAGT+TGG - chr2_3:3376857-3376876 None:intergenic 45.0%
CACGACAGAGGTAACATGTT+TGG - chr2_3:3378610-3378629 None:intergenic 45.0%
ATGCCAAGTATACACGACAG+AGG - chr2_3:3378622-3378641 None:intergenic 45.0%
AATGGGAGAGTCTACGGAGA+AGG + chr2_3:3376631-3376650 Msa0271860:exon 50.0%
GATCGACACTCGCAGTAGAA+AGG - chr2_3:3376740-3376759 None:intergenic 50.0%
TAGAGAAGGAGTTTCGCGCT+TGG - chr2_3:3376762-3376781 None:intergenic 50.0%
TGCTTCGTTCTTCGTTGCTC+AGG + chr2_3:3376898-3376917 Msa0271860:CDS 50.0%
TGACACGTGGCACCTCATTT+AGG + chr2_3:3377263-3377282 Msa0271860:intron 50.0%
GACACGTGGCACCTCATTTA+GGG + chr2_3:3377264-3377283 Msa0271860:intron 50.0%
ACACTGAATTTGATGGGGCG+GGG - chr2_3:3378467-3378486 None:intergenic 50.0%
GACACTGAATTTGATGGGGC+GGG - chr2_3:3378468-3378487 None:intergenic 50.0%
GGACACTGAATTTGATGGGG+CGG - chr2_3:3378469-3378488 None:intergenic 50.0%
AATGGGAGAGTCTACGGAGA+AGG + chr2_3:3376631-3376650 Msa0271860:exon 50.0%
GATCGACACTCGCAGTAGAA+AGG - chr2_3:3376740-3376759 None:intergenic 50.0%
TAGAGAAGGAGTTTCGCGCT+TGG - chr2_3:3376762-3376781 None:intergenic 50.0%
TGCTTCGTTCTTCGTTGCTC+AGG + chr2_3:3376898-3376917 Msa0271860:CDS 50.0%
TGACACGTGGCACCTCATTT+AGG + chr2_3:3377263-3377282 Msa0271860:intron 50.0%
GACACGTGGCACCTCATTTA+GGG + chr2_3:3377264-3377283 Msa0271860:intron 50.0%
ACACTGAATTTGATGGGGCG+GGG - chr2_3:3378467-3378486 None:intergenic 50.0%
GACACTGAATTTGATGGGGC+GGG - chr2_3:3378468-3378487 None:intergenic 50.0%
GGACACTGAATTTGATGGGG+CGG - chr2_3:3378469-3378488 None:intergenic 50.0%
CGCTTCTCGATCTCCAACTG+CGG + chr2_3:3376841-3376860 Msa0271860:CDS 55.0%
!! CTTTGGCAGTTTTGGCCCCA+AGG + chr2_3:3377222-3377241 Msa0271860:intron 55.0%
CTGACGTGGCACCCTAAATG+AGG - chr2_3:3377278-3377297 None:intergenic 55.0%
! GGGGCTGTAACCGCAAGTTT+TGG + chr2_3:3377363-3377382 Msa0271860:intron 55.0%
TGAATTTGATGGGGCGGGGT+TGG - chr2_3:3378463-3378482 None:intergenic 55.0%
CGCTTCTCGATCTCCAACTG+CGG + chr2_3:3376841-3376860 Msa0271860:CDS 55.0%
!! CTTTGGCAGTTTTGGCCCCA+AGG + chr2_3:3377222-3377241 Msa0271860:intron 55.0%
CTGACGTGGCACCCTAAATG+AGG - chr2_3:3377278-3377297 None:intergenic 55.0%
! GGGGCTGTAACCGCAAGTTT+TGG + chr2_3:3377363-3377382 Msa0271860:intron 55.0%
TGAATTTGATGGGGCGGGGT+TGG - chr2_3:3378463-3378482 None:intergenic 55.0%
! TTAGGGTGCCACGTCAGCGT+TGG + chr2_3:3377281-3377300 Msa0271860:intron 60.0%
! TAGGGTGCCACGTCAGCGTT+GGG + chr2_3:3377282-3377301 Msa0271860:intron 60.0%
! TTAGGGTGCCACGTCAGCGT+TGG + chr2_3:3377281-3377300 Msa0271860:intron 60.0%
! TAGGGTGCCACGTCAGCGTT+GGG + chr2_3:3377282-3377301 Msa0271860:intron 60.0%
! AGGGTGCCACGTCAGCGTTG+GGG + chr2_3:3377283-3377302 Msa0271860:intron 65.0%
TTTGGCCCCCAACGCTGACG+TGG - chr2_3:3377292-3377311 None:intergenic 65.0%
!! GGGGCGGGGTTGGAAGTATC+TGG - chr2_3:3378453-3378472 None:intergenic 65.0%
! AGGGTGCCACGTCAGCGTTG+GGG + chr2_3:3377283-3377302 Msa0271860:intron 65.0%
TTTGGCCCCCAACGCTGACG+TGG - chr2_3:3377292-3377311 None:intergenic 65.0%
!! GGGGCGGGGTTGGAAGTATC+TGG - chr2_3:3378453-3378472 None:intergenic 65.0%
! GGGTGCCACGTCAGCGTTGG+GGG + chr2_3:3377284-3377303 Msa0271860:intron 70.0%
! GGGTGCCACGTCAGCGTTGG+GGG + chr2_3:3377284-3377303 Msa0271860:intron 70.0%
Chromosome Type Strat End Strand Name
chr2_3 gene 3376575 3378722 3376575 ID=Msa0271860;Name=Msa0271860
chr2_3 mRNA 3376575 3378722 3376575 ID=Msa0271860-mRNA-1;Parent=Msa0271860;Name=Msa0271860-mRNA-1;_AED=0.06;_eAED=0.06;_QI=57|1|1|1|1|1|3|228|197
chr2_3 exon 3376575 3376919 3376575 ID=Msa0271860-mRNA-1:exon:1632;Parent=Msa0271860-mRNA-1
chr2_3 exon 3377004 3377120 3377004 ID=Msa0271860-mRNA-1:exon:1633;Parent=Msa0271860-mRNA-1
chr2_3 exon 3378306 3378722 3378306 ID=Msa0271860-mRNA-1:exon:1634;Parent=Msa0271860-mRNA-1
chr2_3 five_prime_UTR 3376575 3376631 3376575 ID=Msa0271860-mRNA-1:five_prime_utr;Parent=Msa0271860-mRNA-1
chr2_3 CDS 3376632 3376919 3376632 ID=Msa0271860-mRNA-1:cds;Parent=Msa0271860-mRNA-1
chr2_3 CDS 3377004 3377120 3377004 ID=Msa0271860-mRNA-1:cds;Parent=Msa0271860-mRNA-1
chr2_3 CDS 3378306 3378494 3378306 ID=Msa0271860-mRNA-1:cds;Parent=Msa0271860-mRNA-1
chr2_3 three_prime_UTR 3378495 3378722 3378495 ID=Msa0271860-mRNA-1:three_prime_utr;Parent=Msa0271860-mRNA-1
Gene Sequence

>Msa0271860

ATGGGAGAGTCTACGGAGAAGGCGAAGTCAGAAGCAGTGAAGATAATAGAATCATTCGAAGTTCTTCCAAAACTCGTTGTTTTCGATCTCGATTACACTCTCTGGCCTTTCTACTGCGAGTGTCGATCCAAGCGCGAAACTCCTTCTCTATATCATCACGCTATGGGAATCTTGTTGGCGCTTAAACACAAAGGAATTGATATTGCAATCGCTTCTCGATCTCCAACTGCGGATATTGCTAAAGCTTTTCTTAATAAATTGGGAATTGCTTCGTTCTTCGTTGCTCAGGAAATATATTCGAGTTGGACTCATAAAACGGATCATTTTCAGCGAATTCATTCTACTACTGGAGTTCCGTTTAGCTCTATGCTCTTTTTTGATGATGAGAATAGAAACATCCAAGCGGTCTCTAAAATGGGAGTGACTAGCATTTTGGTTGATAATGGGGTAAATCTTGGAGCCTTATCTAAAGGTCTTACACAGTTTTCTCGAAACTGGAACACATCACAGAAGAACAAGCAGAAATGGCTATCCGATTATTCTAAGAAGCCAGATACTTCCAACCCCGCCCCATCAAATTCAGTGTCCAAGTAA
Protein sequence

>Msa0271860

MGESTEKAKSEAVKIIESFEVLPKLVVFDLDYTLWPFYCECRSKRETPSLYHHAMGILLALKHKGIDIAIASRSPTADIAKAFLNKLGIASFFVAQEIYSSWTHKTDHFQRIHSTTGVPFSSMLFFDDENRNIQAVSKMGVTSILVDNGVNLGALSKGLTQFSRNWNTSQKNKQKWLSDYSKKPDTSNPAPSNSVSK*