AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0310830

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0310830 MtrunA17_Chr2g0279611 94.416 197 11 0 1 197 1 197 1.53e-141 392
Msa0310830 MtrunA17_Chr4g0049871 68.208 173 54 1 3 175 2 173 6.25e-86 251
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0310830 AT2G14110.1 68.132 182 58 0 6 187 4 185 3.41e-94 273
Msa0310830 AT2G14110.2 69.925 133 40 0 4 136 2 134 4.52e-70 210

Find 48 sgRNAs with CRISPR-Local

Find 256 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTACATATAGGTCTCTAAAA+TGG 0.207038 2_4:+5651955 Msa0310830:intron
AATGGGAGTGACTAGCATTT+TGG 0.255669 2_4:+5651973 Msa0310830:CDS
AGATCGAAAACAACGAGTTT+TGG 0.286511 2_4:-5650356 None:intergenic
GTTGATAATGGGGTAAATCT+TGG 0.322461 2_4:+5651995 Msa0310830:CDS
CTAGTATGATTATAGAAAAC+AGG 0.336024 2_4:-5652354 None:intergenic
ATCTGGCTTCTTAGAATAAT+CGG 0.355976 2_4:-5652092 None:intergenic
TACTTGGACACTGAATTTGA+TGG 0.360359 2_4:-5652130 None:intergenic
TGCTTCGTTCTTCGTTGCTC+AGG 0.372516 2_4:+5650556 Msa0310830:CDS
GGGGCGGGGTTGGAAGTATC+TGG 0.373055 2_4:-5652109 None:intergenic
CAGCGAATTCATTCTACTAC+TGG 0.376753 2_4:+5650701 Msa0310830:CDS
GCTCAATCAAATTAATCGTT+GGG 0.398355 2_4:+5650242 Msa0310830:five_prime_UTR
TGAATTTGATGGGGCGGGGT+TGG 0.401193 2_4:-5652119 None:intergenic
CACAGAAGAACAAGCAGAAA+TGG 0.408899 2_4:+5652065 Msa0310830:CDS
CGCTCAATCAAATTAATCGT+TGG 0.422131 2_4:+5650241 Msa0310830:five_prime_UTR
CACGACAGAGGTAACATGTT+TGG 0.437740 2_4:-5652266 None:intergenic
GAGCAGAGAGAAAGCGAAAT+GGG 0.444666 2_4:+5650272 Msa0310830:exon
AGAGCAGAGAGAAAGCGAAA+TGG 0.444737 2_4:+5650271 Msa0310830:exon
TACATATAGGTCTCTAAAAT+GGG 0.446794 2_4:+5651956 Msa0310830:intron
TCACGCTATGGGAATCTTGT+TGG 0.449058 2_4:+5650445 Msa0310830:CDS
TTCAGGAAATATATTCGAGT+TGG 0.451128 2_4:+5650657 Msa0310830:intron
TCGATCTCGATTACACTCTC+TGG 0.456392 2_4:+5650372 Msa0310830:CDS
TTCTCTATATCATCACGCTA+TGG 0.459033 2_4:+5650433 Msa0310830:CDS
GACACTGAATTTGATGGGGC+GGG 0.459346 2_4:-5652124 None:intergenic
ACTTGGACACTGAATTTGAT+GGG 0.473301 2_4:-5652129 None:intergenic
TTACCTCTGTCGTGTATACT+TGG 0.484966 2_4:+5652275 Msa0310830:three_prime_UTR
AAGCGAAATGGGAGAGTCTA+CGG 0.501518 2_4:+5650283 Msa0310830:exon
TCTCTATATCATCACGCTAT+GGG 0.504273 2_4:+5650434 Msa0310830:CDS
ATTGAGACTACAATTCAAGT+AGG 0.515093 2_4:-5652229 None:intergenic
GATCGACACTCGCAGTAGAA+AGG 0.521923 2_4:-5650395 None:intergenic
TAGAGAAGGAGTTTCGCGCT+TGG 0.523146 2_4:-5650417 None:intergenic
CTGTGTAAGACCTTTAGATA+AGG 0.524076 2_4:-5652020 None:intergenic
GCTTTAGCAATATCCGCAGT+TGG 0.525120 2_4:-5650512 None:intergenic
TTCGAGTTGGACTCATAAAA+CGG 0.526393 2_4:+5650670 Msa0310830:CDS
AATCTTGGAGCCTTATCTAA+AGG 0.528346 2_4:+5652010 Msa0310830:CDS
CTCTAAATACATGAATATCA+AGG 0.549756 2_4:-5652330 None:intergenic
CTCAATCAAATTAATCGTTG+GGG 0.565833 2_4:+5650243 Msa0310830:five_prime_UTR
ACACTGAATTTGATGGGGCG+GGG 0.585099 2_4:-5652123 None:intergenic
AGTCTCAATCAAATTACTGA+AGG 0.588172 2_4:+5652243 Msa0310830:three_prime_UTR
GGACACTGAATTTGATGGGG+CGG 0.589207 2_4:-5652125 None:intergenic
AATGGGAGAGTCTACGGAGA+AGG 0.592110 2_4:+5650289 Msa0310830:exon
CTTGGACACTGAATTTGATG+GGG 0.607262 2_4:-5652128 None:intergenic
CGCTTCTCGATCTCCAACTG+CGG 0.610594 2_4:+5650499 Msa0310830:CDS
ATAGCGTGATGATATAGAGA+AGG 0.642803 2_4:-5650431 None:intergenic
TAGTATGATTATAGAAAACA+GGG 0.650221 2_4:-5652353 None:intergenic
TTGTTGGCGCTTAAACACAA+AGG 0.670200 2_4:+5650461 Msa0310830:CDS
TCAATCAAATTAATCGTTGG+GGG 0.692972 2_4:+5650244 Msa0310830:five_prime_UTR
TGAGAATAGAAACATCCAAG+CGG 0.730982 2_4:+5650757 Msa0310830:CDS
ATGCCAAGTATACACGACAG+AGG 0.736619 2_4:-5652278 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TGTCATTTTTTTTTTTTTTT+TGG - chr2_4:5650906-5650925 None:intergenic 10.0%
!!! TTTTGTATTTAAATTAGTTA+GGG + chr2_4:5651000-5651019 Msa0310830:intron 10.0%
!!! TGTCATTTTTTTTTTTTTTT+TGG - chr2_4:5650906-5650925 None:intergenic 10.0%
!!! TTTTGTATTTAAATTAGTTA+GGG + chr2_4:5651000-5651019 Msa0310830:intron 10.0%
!!! TAAAGCTTTTCTTAATAAAT+TGG + chr2_4:5650529-5650548 Msa0310830:CDS 15.0%
!!! AAAGCTTTTCTTAATAAATT+GGG + chr2_4:5650530-5650549 Msa0310830:CDS 15.0%
!!! GTTTTGTATTTAAATTAGTT+AGG + chr2_4:5650999-5651018 Msa0310830:intron 15.0%
!! TTTGTATTTAAATTAGTTAG+GGG + chr2_4:5651001-5651020 Msa0310830:intron 15.0%
!! AAAAAAAAAATTGATTATGC+AGG + chr2_4:5651151-5651170 Msa0310830:intron 15.0%
!!! TAAAGCTTTTCTTAATAAAT+TGG + chr2_4:5650529-5650548 Msa0310830:CDS 15.0%
!!! AAAGCTTTTCTTAATAAATT+GGG + chr2_4:5650530-5650549 Msa0310830:CDS 15.0%
!!! GTTTTGTATTTAAATTAGTT+AGG + chr2_4:5650999-5651018 Msa0310830:intron 15.0%
!! TTTGTATTTAAATTAGTTAG+GGG + chr2_4:5651001-5651020 Msa0310830:intron 15.0%
!! AAAAAAAAAATTGATTATGC+AGG + chr2_4:5651151-5651170 Msa0310830:intron 15.0%
!! TGAACAAAACAACAAAAATT+AGG - chr2_4:5650641-5650660 None:intergenic 20.0%
!!! TAATTTTTGTTGTTTTGTTC+AGG + chr2_4:5650640-5650659 Msa0310830:intron 20.0%
!!! AAGATTAGTTAAAATCACAT+TGG - chr2_4:5650863-5650882 None:intergenic 20.0%
!!! ATGTGATTTTAACTAATCTT+TGG + chr2_4:5650863-5650882 Msa0310830:intron 20.0%
!!! TTTTTTTTTTTTTTGGCCTT+GGG - chr2_4:5650899-5650918 None:intergenic 20.0%
!!! TTTTTTTTTTTTTTTGGCCT+TGG - chr2_4:5650900-5650919 None:intergenic 20.0%
!! AAAAAAAAAAAAATGACACG+TGG + chr2_4:5650908-5650927 Msa0310830:intron 20.0%
!! TTGTATTTAAATTAGTTAGG+GGG + chr2_4:5651002-5651021 Msa0310830:intron 20.0%
!! AATGCTAAAAACAATTATAG+AGG - chr2_4:5651237-5651256 None:intergenic 20.0%
!!! AATCAATTTATAGCTTGTTT+TGG + chr2_4:5651339-5651358 Msa0310830:intron 20.0%
!!! TTACAATTCTAGTTTTAAGT+TGG + chr2_4:5651520-5651539 Msa0310830:intron 20.0%
!! AGTAAATTTGACAAAATCAT+GGG - chr2_4:5651833-5651852 None:intergenic 20.0%
!! AAGTAAATTTGACAAAATCA+TGG - chr2_4:5651834-5651853 None:intergenic 20.0%
!! TTATGTAAAAGTTACTTAGT+TGG - chr2_4:5651922-5651941 None:intergenic 20.0%
!! TTGTTTATATCGTTACATAT+AGG + chr2_4:5651943-5651962 Msa0310830:intron 20.0%
!!! AATCCAAAGAATTTTTTACT+TGG - chr2_4:5652149-5652168 None:intergenic 20.0%
!! TAGTATGATTATAGAAAACA+GGG - chr2_4:5652356-5652375 None:intergenic 20.0%
!! TGAACAAAACAACAAAAATT+AGG - chr2_4:5650641-5650660 None:intergenic 20.0%
!!! TAATTTTTGTTGTTTTGTTC+AGG + chr2_4:5650640-5650659 Msa0310830:intron 20.0%
!!! AAGATTAGTTAAAATCACAT+TGG - chr2_4:5650863-5650882 None:intergenic 20.0%
!!! ATGTGATTTTAACTAATCTT+TGG + chr2_4:5650863-5650882 Msa0310830:intron 20.0%
!!! TTTTTTTTTTTTTTGGCCTT+GGG - chr2_4:5650899-5650918 None:intergenic 20.0%
!!! TTTTTTTTTTTTTTTGGCCT+TGG - chr2_4:5650900-5650919 None:intergenic 20.0%
!! AAAAAAAAAAAAATGACACG+TGG + chr2_4:5650908-5650927 Msa0310830:intron 20.0%
!! TTGTATTTAAATTAGTTAGG+GGG + chr2_4:5651002-5651021 Msa0310830:intron 20.0%
!! AATGCTAAAAACAATTATAG+AGG - chr2_4:5651237-5651256 None:intergenic 20.0%
!!! AATCAATTTATAGCTTGTTT+TGG + chr2_4:5651339-5651358 Msa0310830:intron 20.0%
!!! TTACAATTCTAGTTTTAAGT+TGG + chr2_4:5651520-5651539 Msa0310830:intron 20.0%
!! AGTAAATTTGACAAAATCAT+GGG - chr2_4:5651833-5651852 None:intergenic 20.0%
!! AAGTAAATTTGACAAAATCA+TGG - chr2_4:5651834-5651853 None:intergenic 20.0%
!! TTATGTAAAAGTTACTTAGT+TGG - chr2_4:5651922-5651941 None:intergenic 20.0%
!! TTGTTTATATCGTTACATAT+AGG + chr2_4:5651943-5651962 Msa0310830:intron 20.0%
!!! AATCCAAAGAATTTTTTACT+TGG - chr2_4:5652149-5652168 None:intergenic 20.0%
!! TAGTATGATTATAGAAAACA+GGG - chr2_4:5652356-5652375 None:intergenic 20.0%
!!! TTTTTTTTTTTTTGGCCTTG+GGG - chr2_4:5650898-5650917 None:intergenic 25.0%
!!! AAACTCTAGAAGTAGTTTTT+AGG - chr2_4:5651076-5651095 None:intergenic 25.0%
! AGAATAAATTGATTATGCAG+TGG + chr2_4:5651120-5651139 Msa0310830:intron 25.0%
!!! TTTAGCTTGAGATTAGAATA+TGG + chr2_4:5651203-5651222 Msa0310830:intron 25.0%
!! TTGTCTAAAAAATGAGTTGA+AGG - chr2_4:5651319-5651338 None:intergenic 25.0%
! TGAGTTCAACAATAAATTTG+AGG + chr2_4:5651471-5651490 Msa0310830:intron 25.0%
! GAGTTCAACAATAAATTTGA+GGG + chr2_4:5651472-5651491 Msa0310830:intron 25.0%
! CAACATAAGCTTATGAATAA+AGG - chr2_4:5651725-5651744 None:intergenic 25.0%
! TAAATTTGACAAAATCATGG+GGG - chr2_4:5651831-5651850 None:intergenic 25.0%
! GTAAATTTGACAAAATCATG+GGG - chr2_4:5651832-5651851 None:intergenic 25.0%
!! TATATCATGTGCATCATTTT+TGG - chr2_4:5651862-5651881 None:intergenic 25.0%
! AGTTACTTAGTTGGAAAAAT+GGG - chr2_4:5651913-5651932 None:intergenic 25.0%
! AAGTTACTTAGTTGGAAAAA+TGG - chr2_4:5651914-5651933 None:intergenic 25.0%
!! TTACATATAGGTCTCTAAAA+TGG + chr2_4:5651955-5651974 Msa0310830:intron 25.0%
!!! TACATATAGGTCTCTAAAAT+GGG + chr2_4:5651956-5651975 Msa0310830:intron 25.0%
! TGTCCAAGTAAAAAATTCTT+TGG + chr2_4:5652143-5652162 Msa0310830:exon 25.0%
! CTCTAAATACATGAATATCA+AGG - chr2_4:5652333-5652352 None:intergenic 25.0%
! CTAGTATGATTATAGAAAAC+AGG - chr2_4:5652357-5652376 None:intergenic 25.0%
!!! TTTTTTTTTTTTTGGCCTTG+GGG - chr2_4:5650898-5650917 None:intergenic 25.0%
!!! AAACTCTAGAAGTAGTTTTT+AGG - chr2_4:5651076-5651095 None:intergenic 25.0%
! AGAATAAATTGATTATGCAG+TGG + chr2_4:5651120-5651139 Msa0310830:intron 25.0%
!!! TTTAGCTTGAGATTAGAATA+TGG + chr2_4:5651203-5651222 Msa0310830:intron 25.0%
!! TTGTCTAAAAAATGAGTTGA+AGG - chr2_4:5651319-5651338 None:intergenic 25.0%
! TGAGTTCAACAATAAATTTG+AGG + chr2_4:5651471-5651490 Msa0310830:intron 25.0%
! GAGTTCAACAATAAATTTGA+GGG + chr2_4:5651472-5651491 Msa0310830:intron 25.0%
! CAACATAAGCTTATGAATAA+AGG - chr2_4:5651725-5651744 None:intergenic 25.0%
! TAAATTTGACAAAATCATGG+GGG - chr2_4:5651831-5651850 None:intergenic 25.0%
! GTAAATTTGACAAAATCATG+GGG - chr2_4:5651832-5651851 None:intergenic 25.0%
!! TATATCATGTGCATCATTTT+TGG - chr2_4:5651862-5651881 None:intergenic 25.0%
! AGTTACTTAGTTGGAAAAAT+GGG - chr2_4:5651913-5651932 None:intergenic 25.0%
! AAGTTACTTAGTTGGAAAAA+TGG - chr2_4:5651914-5651933 None:intergenic 25.0%
!! TTACATATAGGTCTCTAAAA+TGG + chr2_4:5651955-5651974 Msa0310830:intron 25.0%
!!! TACATATAGGTCTCTAAAAT+GGG + chr2_4:5651956-5651975 Msa0310830:intron 25.0%
! TGTCCAAGTAAAAAATTCTT+TGG + chr2_4:5652143-5652162 Msa0310830:exon 25.0%
! CTCTAAATACATGAATATCA+AGG - chr2_4:5652333-5652352 None:intergenic 25.0%
! CTAGTATGATTATAGAAAAC+AGG - chr2_4:5652357-5652376 None:intergenic 25.0%
GCTCAATCAAATTAATCGTT+GGG + chr2_4:5650242-5650261 Msa0310830:five_prime_UTR 30.0%
CTCAATCAAATTAATCGTTG+GGG + chr2_4:5650243-5650262 Msa0310830:five_prime_UTR 30.0%
TCAATCAAATTAATCGTTGG+GGG + chr2_4:5650244-5650263 Msa0310830:five_prime_UTR 30.0%
TTCAGGAAATATATTCGAGT+TGG + chr2_4:5650657-5650676 Msa0310830:intron 30.0%
! AAAAAGAGCATAGAGCTAAA+CGG - chr2_4:5650731-5650750 None:intergenic 30.0%
GATTAATGATTATTACCGCT+TGG - chr2_4:5650775-5650794 None:intergenic 30.0%
ATAGTGTATGACTGTATCTT+TGG + chr2_4:5650808-5650827 Msa0310830:intron 30.0%
! TAACTAATCTTTGGCAGTTT+TGG + chr2_4:5650872-5650891 Msa0310830:intron 30.0%
! TATTGAATCTTTGGCAGTTT+TGG - chr2_4:5650968-5650987 None:intergenic 30.0%
TGCCAAAGATTCAATACTTA+GGG + chr2_4:5650972-5650991 Msa0310830:intron 30.0%
! TTTAGGCTTAATTGCACTTT+TGG - chr2_4:5651059-5651078 None:intergenic 30.0%
GGAATTGATTATGACATGTT+TGG - chr2_4:5651574-5651593 None:intergenic 30.0%
GTAAGTTGTTTATCCAAACA+CGG + chr2_4:5651756-5651775 Msa0310830:intron 30.0%
ATTATGAATAAGACCGTGTT+TGG - chr2_4:5651772-5651791 None:intergenic 30.0%
GTTACTTAGTTGGAAAAATG+GGG - chr2_4:5651912-5651931 None:intergenic 30.0%
!!! ACTAGCATTTTGGTTGATAA+TGG + chr2_4:5651983-5652002 Msa0310830:CDS 30.0%
!!! CTAGCATTTTGGTTGATAAT+GGG + chr2_4:5651984-5652003 Msa0310830:CDS 30.0%
!!! TAGCATTTTGGTTGATAATG+GGG + chr2_4:5651985-5652004 Msa0310830:CDS 30.0%
! ATCTGGCTTCTTAGAATAAT+CGG - chr2_4:5652095-5652114 None:intergenic 30.0%
ATTGAGACTACAATTCAAGT+AGG - chr2_4:5652232-5652251 None:intergenic 30.0%
AGTCTCAATCAAATTACTGA+AGG + chr2_4:5652243-5652262 Msa0310830:three_prime_UTR 30.0%
GCTCAATCAAATTAATCGTT+GGG + chr2_4:5650242-5650261 Msa0310830:five_prime_UTR 30.0%
CTCAATCAAATTAATCGTTG+GGG + chr2_4:5650243-5650262 Msa0310830:five_prime_UTR 30.0%
TCAATCAAATTAATCGTTGG+GGG + chr2_4:5650244-5650263 Msa0310830:five_prime_UTR 30.0%
TTCAGGAAATATATTCGAGT+TGG + chr2_4:5650657-5650676 Msa0310830:intron 30.0%
! AAAAAGAGCATAGAGCTAAA+CGG - chr2_4:5650731-5650750 None:intergenic 30.0%
GATTAATGATTATTACCGCT+TGG - chr2_4:5650775-5650794 None:intergenic 30.0%
ATAGTGTATGACTGTATCTT+TGG + chr2_4:5650808-5650827 Msa0310830:intron 30.0%
! TAACTAATCTTTGGCAGTTT+TGG + chr2_4:5650872-5650891 Msa0310830:intron 30.0%
! TATTGAATCTTTGGCAGTTT+TGG - chr2_4:5650968-5650987 None:intergenic 30.0%
TGCCAAAGATTCAATACTTA+GGG + chr2_4:5650972-5650991 Msa0310830:intron 30.0%
! TTTAGGCTTAATTGCACTTT+TGG - chr2_4:5651059-5651078 None:intergenic 30.0%
GGAATTGATTATGACATGTT+TGG - chr2_4:5651574-5651593 None:intergenic 30.0%
GTAAGTTGTTTATCCAAACA+CGG + chr2_4:5651756-5651775 Msa0310830:intron 30.0%
ATTATGAATAAGACCGTGTT+TGG - chr2_4:5651772-5651791 None:intergenic 30.0%
GTTACTTAGTTGGAAAAATG+GGG - chr2_4:5651912-5651931 None:intergenic 30.0%
!!! ACTAGCATTTTGGTTGATAA+TGG + chr2_4:5651983-5652002 Msa0310830:CDS 30.0%
!!! CTAGCATTTTGGTTGATAAT+GGG + chr2_4:5651984-5652003 Msa0310830:CDS 30.0%
!!! TAGCATTTTGGTTGATAATG+GGG + chr2_4:5651985-5652004 Msa0310830:CDS 30.0%
! ATCTGGCTTCTTAGAATAAT+CGG - chr2_4:5652095-5652114 None:intergenic 30.0%
ATTGAGACTACAATTCAAGT+AGG - chr2_4:5652232-5652251 None:intergenic 30.0%
AGTCTCAATCAAATTACTGA+AGG + chr2_4:5652243-5652262 Msa0310830:three_prime_UTR 30.0%
CGCTCAATCAAATTAATCGT+TGG + chr2_4:5650241-5650260 Msa0310830:five_prime_UTR 35.0%
! AGATCGAAAACAACGAGTTT+TGG - chr2_4:5650359-5650378 None:intergenic 35.0%
ATAGCGTGATGATATAGAGA+AGG - chr2_4:5650434-5650453 None:intergenic 35.0%
TTCTCTATATCATCACGCTA+TGG + chr2_4:5650433-5650452 Msa0310830:CDS 35.0%
TCTCTATATCATCACGCTAT+GGG + chr2_4:5650434-5650453 Msa0310830:CDS 35.0%
!! TTCGAGTTGGACTCATAAAA+CGG + chr2_4:5650670-5650689 Msa0310830:CDS 35.0%
TGAGAATAGAAACATCCAAG+CGG + chr2_4:5650757-5650776 Msa0310830:CDS 35.0%
TGACTGTATCTTTGGATTGA+TGG + chr2_4:5650816-5650835 Msa0310830:intron 35.0%
CTGCCAAAGATTCAATACTT+AGG + chr2_4:5650971-5650990 Msa0310830:intron 35.0%
GCCAAAGATTCAATACTTAG+GGG + chr2_4:5650973-5650992 Msa0310830:intron 35.0%
CCAAAGATTCAATACTTAGG+GGG + chr2_4:5650974-5650993 Msa0310830:intron 35.0%
CTCTATCTTTCCAAAACTTG+CGG - chr2_4:5651034-5651053 None:intergenic 35.0%
!!! GCAAGTTTTGGAAAGATAGA+GGG + chr2_4:5651033-5651052 Msa0310830:intron 35.0%
!! GGATTGACTTGATGTTTGTT+TGG + chr2_4:5651360-5651379 Msa0310830:intron 35.0%
!! TTCGACTAGAATTGAGTTGA+AGG + chr2_4:5651412-5651431 Msa0310830:intron 35.0%
! CAAAATTGATTCTAGAGGTG+TGG - chr2_4:5651595-5651614 None:intergenic 35.0%
!!! CACACCTCTAGAATCAATTT+TGG + chr2_4:5651593-5651612 Msa0310830:intron 35.0%
ACCAATTTGAGCTTATCTTC+TGG + chr2_4:5651632-5651651 Msa0310830:intron 35.0%
TCCAGAAGATAAGCTCAAAT+TGG - chr2_4:5651636-5651655 None:intergenic 35.0%
!! GTTGATAATGGGGTAAATCT+TGG + chr2_4:5651995-5652014 Msa0310830:CDS 35.0%
! AATCTTGGAGCCTTATCTAA+AGG + chr2_4:5652010-5652029 Msa0310830:CDS 35.0%
CTGTGTAAGACCTTTAGATA+AGG - chr2_4:5652023-5652042 None:intergenic 35.0%
! TTACACAGTTTTCTCGAAAC+TGG + chr2_4:5652035-5652054 Msa0310830:CDS 35.0%
ACTTGGACACTGAATTTGAT+GGG - chr2_4:5652132-5652151 None:intergenic 35.0%
TACTTGGACACTGAATTTGA+TGG - chr2_4:5652133-5652152 None:intergenic 35.0%
CGCTCAATCAAATTAATCGT+TGG + chr2_4:5650241-5650260 Msa0310830:five_prime_UTR 35.0%
! AGATCGAAAACAACGAGTTT+TGG - chr2_4:5650359-5650378 None:intergenic 35.0%
ATAGCGTGATGATATAGAGA+AGG - chr2_4:5650434-5650453 None:intergenic 35.0%
TTCTCTATATCATCACGCTA+TGG + chr2_4:5650433-5650452 Msa0310830:CDS 35.0%
TCTCTATATCATCACGCTAT+GGG + chr2_4:5650434-5650453 Msa0310830:CDS 35.0%
!! TTCGAGTTGGACTCATAAAA+CGG + chr2_4:5650670-5650689 Msa0310830:CDS 35.0%
TGAGAATAGAAACATCCAAG+CGG + chr2_4:5650757-5650776 Msa0310830:CDS 35.0%
TGACTGTATCTTTGGATTGA+TGG + chr2_4:5650816-5650835 Msa0310830:intron 35.0%
CTGCCAAAGATTCAATACTT+AGG + chr2_4:5650971-5650990 Msa0310830:intron 35.0%
GCCAAAGATTCAATACTTAG+GGG + chr2_4:5650973-5650992 Msa0310830:intron 35.0%
CCAAAGATTCAATACTTAGG+GGG + chr2_4:5650974-5650993 Msa0310830:intron 35.0%
CTCTATCTTTCCAAAACTTG+CGG - chr2_4:5651034-5651053 None:intergenic 35.0%
!!! GCAAGTTTTGGAAAGATAGA+GGG + chr2_4:5651033-5651052 Msa0310830:intron 35.0%
!! GGATTGACTTGATGTTTGTT+TGG + chr2_4:5651360-5651379 Msa0310830:intron 35.0%
!! TTCGACTAGAATTGAGTTGA+AGG + chr2_4:5651412-5651431 Msa0310830:intron 35.0%
! CAAAATTGATTCTAGAGGTG+TGG - chr2_4:5651595-5651614 None:intergenic 35.0%
!!! CACACCTCTAGAATCAATTT+TGG + chr2_4:5651593-5651612 Msa0310830:intron 35.0%
ACCAATTTGAGCTTATCTTC+TGG + chr2_4:5651632-5651651 Msa0310830:intron 35.0%
TCCAGAAGATAAGCTCAAAT+TGG - chr2_4:5651636-5651655 None:intergenic 35.0%
!! GTTGATAATGGGGTAAATCT+TGG + chr2_4:5651995-5652014 Msa0310830:CDS 35.0%
! AATCTTGGAGCCTTATCTAA+AGG + chr2_4:5652010-5652029 Msa0310830:CDS 35.0%
CTGTGTAAGACCTTTAGATA+AGG - chr2_4:5652023-5652042 None:intergenic 35.0%
! TTACACAGTTTTCTCGAAAC+TGG + chr2_4:5652035-5652054 Msa0310830:CDS 35.0%
ACTTGGACACTGAATTTGAT+GGG - chr2_4:5652132-5652151 None:intergenic 35.0%
TACTTGGACACTGAATTTGA+TGG - chr2_4:5652133-5652152 None:intergenic 35.0%
TTGTTGGCGCTTAAACACAA+AGG + chr2_4:5650461-5650480 Msa0310830:CDS 40.0%
CAGCGAATTCATTCTACTAC+TGG + chr2_4:5650701-5650720 Msa0310830:CDS 40.0%
!! CCCCCTAAGTATTGAATCTT+TGG - chr2_4:5650977-5650996 None:intergenic 40.0%
!!! CGCAAGTTTTGGAAAGATAG+AGG + chr2_4:5651032-5651051 Msa0310830:intron 40.0%
! GGAGCCAAAATTGATTCTAG+AGG - chr2_4:5651600-5651619 None:intergenic 40.0%
!!! AATTTTGGCTCCTCCAAAAG+TGG + chr2_4:5651608-5651627 Msa0310830:intron 40.0%
!! CAAATTGGTTCCACTTTTGG+AGG - chr2_4:5651621-5651640 None:intergenic 40.0%
!! GCTCAAATTGGTTCCACTTT+TGG - chr2_4:5651624-5651643 None:intergenic 40.0%
! AATGGGAGTGACTAGCATTT+TGG + chr2_4:5651973-5651992 Msa0310830:CDS 40.0%
CACAGAAGAACAAGCAGAAA+TGG + chr2_4:5652065-5652084 Msa0310830:CDS 40.0%
CTTGGACACTGAATTTGATG+GGG - chr2_4:5652131-5652150 None:intergenic 40.0%
TTACCTCTGTCGTGTATACT+TGG + chr2_4:5652275-5652294 Msa0310830:three_prime_UTR 40.0%
TTGTTGGCGCTTAAACACAA+AGG + chr2_4:5650461-5650480 Msa0310830:CDS 40.0%
CAGCGAATTCATTCTACTAC+TGG + chr2_4:5650701-5650720 Msa0310830:CDS 40.0%
!! CCCCCTAAGTATTGAATCTT+TGG - chr2_4:5650977-5650996 None:intergenic 40.0%
!!! CGCAAGTTTTGGAAAGATAG+AGG + chr2_4:5651032-5651051 Msa0310830:intron 40.0%
! GGAGCCAAAATTGATTCTAG+AGG - chr2_4:5651600-5651619 None:intergenic 40.0%
!!! AATTTTGGCTCCTCCAAAAG+TGG + chr2_4:5651608-5651627 Msa0310830:intron 40.0%
!! CAAATTGGTTCCACTTTTGG+AGG - chr2_4:5651621-5651640 None:intergenic 40.0%
!! GCTCAAATTGGTTCCACTTT+TGG - chr2_4:5651624-5651643 None:intergenic 40.0%
! AATGGGAGTGACTAGCATTT+TGG + chr2_4:5651973-5651992 Msa0310830:CDS 40.0%
CACAGAAGAACAAGCAGAAA+TGG + chr2_4:5652065-5652084 Msa0310830:CDS 40.0%
CTTGGACACTGAATTTGATG+GGG - chr2_4:5652131-5652150 None:intergenic 40.0%
TTACCTCTGTCGTGTATACT+TGG + chr2_4:5652275-5652294 Msa0310830:three_prime_UTR 40.0%
AGAGCAGAGAGAAAGCGAAA+TGG + chr2_4:5650271-5650290 Msa0310830:exon 45.0%
GAGCAGAGAGAAAGCGAAAT+GGG + chr2_4:5650272-5650291 Msa0310830:exon 45.0%
AAGCGAAATGGGAGAGTCTA+CGG + chr2_4:5650283-5650302 Msa0310830:exon 45.0%
TCGATCTCGATTACACTCTC+TGG + chr2_4:5650372-5650391 Msa0310830:CDS 45.0%
TCACGCTATGGGAATCTTGT+TGG + chr2_4:5650445-5650464 Msa0310830:CDS 45.0%
GCTTTAGCAATATCCGCAGT+TGG - chr2_4:5650515-5650534 None:intergenic 45.0%
CACGACAGAGGTAACATGTT+TGG - chr2_4:5652269-5652288 None:intergenic 45.0%
ATGCCAAGTATACACGACAG+AGG - chr2_4:5652281-5652300 None:intergenic 45.0%
AGAGCAGAGAGAAAGCGAAA+TGG + chr2_4:5650271-5650290 Msa0310830:exon 45.0%
GAGCAGAGAGAAAGCGAAAT+GGG + chr2_4:5650272-5650291 Msa0310830:exon 45.0%
AAGCGAAATGGGAGAGTCTA+CGG + chr2_4:5650283-5650302 Msa0310830:exon 45.0%
TCGATCTCGATTACACTCTC+TGG + chr2_4:5650372-5650391 Msa0310830:CDS 45.0%
TCACGCTATGGGAATCTTGT+TGG + chr2_4:5650445-5650464 Msa0310830:CDS 45.0%
GCTTTAGCAATATCCGCAGT+TGG - chr2_4:5650515-5650534 None:intergenic 45.0%
CACGACAGAGGTAACATGTT+TGG - chr2_4:5652269-5652288 None:intergenic 45.0%
ATGCCAAGTATACACGACAG+AGG - chr2_4:5652281-5652300 None:intergenic 45.0%
AATGGGAGAGTCTACGGAGA+AGG + chr2_4:5650289-5650308 Msa0310830:exon 50.0%
GATCGACACTCGCAGTAGAA+AGG - chr2_4:5650398-5650417 None:intergenic 50.0%
TAGAGAAGGAGTTTCGCGCT+TGG - chr2_4:5650420-5650439 None:intergenic 50.0%
TGCTTCGTTCTTCGTTGCTC+AGG + chr2_4:5650556-5650575 Msa0310830:CDS 50.0%
TGACACGTGGCACCTCATTT+AGG + chr2_4:5650921-5650940 Msa0310830:intron 50.0%
GACACGTGGCACCTCATTTA+GGG + chr2_4:5650922-5650941 Msa0310830:intron 50.0%
ACACTGAATTTGATGGGGCG+GGG - chr2_4:5652126-5652145 None:intergenic 50.0%
GACACTGAATTTGATGGGGC+GGG - chr2_4:5652127-5652146 None:intergenic 50.0%
GGACACTGAATTTGATGGGG+CGG - chr2_4:5652128-5652147 None:intergenic 50.0%
AATGGGAGAGTCTACGGAGA+AGG + chr2_4:5650289-5650308 Msa0310830:exon 50.0%
GATCGACACTCGCAGTAGAA+AGG - chr2_4:5650398-5650417 None:intergenic 50.0%
TAGAGAAGGAGTTTCGCGCT+TGG - chr2_4:5650420-5650439 None:intergenic 50.0%
TGCTTCGTTCTTCGTTGCTC+AGG + chr2_4:5650556-5650575 Msa0310830:CDS 50.0%
TGACACGTGGCACCTCATTT+AGG + chr2_4:5650921-5650940 Msa0310830:intron 50.0%
GACACGTGGCACCTCATTTA+GGG + chr2_4:5650922-5650941 Msa0310830:intron 50.0%
ACACTGAATTTGATGGGGCG+GGG - chr2_4:5652126-5652145 None:intergenic 50.0%
GACACTGAATTTGATGGGGC+GGG - chr2_4:5652127-5652146 None:intergenic 50.0%
GGACACTGAATTTGATGGGG+CGG - chr2_4:5652128-5652147 None:intergenic 50.0%
CGCTTCTCGATCTCCAACTG+CGG + chr2_4:5650499-5650518 Msa0310830:CDS 55.0%
!! CTTTGGCAGTTTTGGCCCCA+AGG + chr2_4:5650880-5650899 Msa0310830:intron 55.0%
CTGACGTGGCACCCTAAATG+AGG - chr2_4:5650936-5650955 None:intergenic 55.0%
! GGGGCTGTAACCGCAAGTTT+TGG + chr2_4:5651021-5651040 Msa0310830:intron 55.0%
TGAATTTGATGGGGCGGGGT+TGG - chr2_4:5652122-5652141 None:intergenic 55.0%
CGCTTCTCGATCTCCAACTG+CGG + chr2_4:5650499-5650518 Msa0310830:CDS 55.0%
!! CTTTGGCAGTTTTGGCCCCA+AGG + chr2_4:5650880-5650899 Msa0310830:intron 55.0%
CTGACGTGGCACCCTAAATG+AGG - chr2_4:5650936-5650955 None:intergenic 55.0%
! GGGGCTGTAACCGCAAGTTT+TGG + chr2_4:5651021-5651040 Msa0310830:intron 55.0%
TGAATTTGATGGGGCGGGGT+TGG - chr2_4:5652122-5652141 None:intergenic 55.0%
! TTAGGGTGCCACGTCAGCGT+TGG + chr2_4:5650939-5650958 Msa0310830:intron 60.0%
! TAGGGTGCCACGTCAGCGTT+GGG + chr2_4:5650940-5650959 Msa0310830:intron 60.0%
! TTAGGGTGCCACGTCAGCGT+TGG + chr2_4:5650939-5650958 Msa0310830:intron 60.0%
! TAGGGTGCCACGTCAGCGTT+GGG + chr2_4:5650940-5650959 Msa0310830:intron 60.0%
! AGGGTGCCACGTCAGCGTTG+GGG + chr2_4:5650941-5650960 Msa0310830:intron 65.0%
TTTGGCCCCCAACGCTGACG+TGG - chr2_4:5650950-5650969 None:intergenic 65.0%
!! GGGGCGGGGTTGGAAGTATC+TGG - chr2_4:5652112-5652131 None:intergenic 65.0%
! AGGGTGCCACGTCAGCGTTG+GGG + chr2_4:5650941-5650960 Msa0310830:intron 65.0%
TTTGGCCCCCAACGCTGACG+TGG - chr2_4:5650950-5650969 None:intergenic 65.0%
!! GGGGCGGGGTTGGAAGTATC+TGG - chr2_4:5652112-5652131 None:intergenic 65.0%
! GGGTGCCACGTCAGCGTTGG+GGG + chr2_4:5650942-5650961 Msa0310830:intron 70.0%
! GGGTGCCACGTCAGCGTTGG+GGG + chr2_4:5650942-5650961 Msa0310830:intron 70.0%
Chromosome Type Strat End Strand Name
chr2_4 gene 5650233 5652381 5650233 ID=Msa0310830;Name=Msa0310830
chr2_4 mRNA 5650233 5652381 5650233 ID=Msa0310830-mRNA-1;Parent=Msa0310830;Name=Msa0310830-mRNA-1;_AED=0.06;_eAED=0.06;_QI=57|1|1|1|1|1|3|228|197
chr2_4 exon 5650233 5650577 5650233 ID=Msa0310830-mRNA-1:exon:2083;Parent=Msa0310830-mRNA-1
chr2_4 exon 5650662 5650778 5650662 ID=Msa0310830-mRNA-1:exon:2084;Parent=Msa0310830-mRNA-1
chr2_4 exon 5651965 5652381 5651965 ID=Msa0310830-mRNA-1:exon:2085;Parent=Msa0310830-mRNA-1
chr2_4 five_prime_UTR 5650233 5650289 5650233 ID=Msa0310830-mRNA-1:five_prime_utr;Parent=Msa0310830-mRNA-1
chr2_4 CDS 5650290 5650577 5650290 ID=Msa0310830-mRNA-1:cds;Parent=Msa0310830-mRNA-1
chr2_4 CDS 5650662 5650778 5650662 ID=Msa0310830-mRNA-1:cds;Parent=Msa0310830-mRNA-1
chr2_4 CDS 5651965 5652153 5651965 ID=Msa0310830-mRNA-1:cds;Parent=Msa0310830-mRNA-1
chr2_4 three_prime_UTR 5652154 5652381 5652154 ID=Msa0310830-mRNA-1:three_prime_utr;Parent=Msa0310830-mRNA-1
Gene Sequence

>Msa0310830

ATGGGAGAGTCTACGGAGAAGGCGAAGTCAGAAGCAGTGAAGATAATAGAATCATTCGAAGTTCTTCCAAAACTCGTTGTTTTCGATCTCGATTACACTCTCTGGCCTTTCTACTGCGAGTGTCGATCCAAGCGCGAAACTCCTTCTCTATATCATCACGCTATGGGAATCTTGTTGGCGCTTAAACACAAAGGAATTGATATTGCAATCGCTTCTCGATCTCCAACTGCGGATATTGCTAAAGCTTTTCTTAATAAATTGGGAATTGCTTCGTTCTTCGTTGCTCAGGAAATATATTCGAGTTGGACTCATAAAACGGATCATTTTCAGCGAATTCATTCTACTACTGGAGTTCCGTTTAGCTCTATGCTCTTTTTTGATGATGAGAATAGAAACATCCAAGCGGTCTCTAAAATGGGAGTGACTAGCATTTTGGTTGATAATGGGGTAAATCTTGGAGCCTTATCTAAAGGTCTTACACAGTTTTCTCGAAACTGGAACACATCACAGAAGAACAAGCAGAAATGGCTATCCGATTATTCTAAGAAGCCAGATACTTCCAACCCCGCCCCATCAAATTCAGTGTCCAAGTAA
Protein sequence

>Msa0310830

MGESTEKAKSEAVKIIESFEVLPKLVVFDLDYTLWPFYCECRSKRETPSLYHHAMGILLALKHKGIDIAIASRSPTADIAKAFLNKLGIASFFVAQEIYSSWTHKTDHFQRIHSTTGVPFSSMLFFDDENRNIQAVSKMGVTSILVDNGVNLGALSKGLTQFSRNWNTSQKNKQKWLSDYSKKPDTSNPAPSNSVSK*