AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0630950


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0630950 MtrunA17_Chr4g0008411 97.462 197 5 0 1 197 1 197 6.00e-139 388
Msa0630950 MtrunA17_Chr4g0008421 72.816 206 47 3 1 201 88 289 1.47e-103 300
Msa0630950 MtrunA17_Chr7g0266341 37.000 200 108 10 11 201 102 292 1.75e-24 97.4
Msa0630950 MtrunA17_Chr2g0306771 37.500 192 94 6 11 201 211 377 3.28e-22 92.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0630950 AT2G37970.1 59.722 216 71 3 1 201 32 246 7.73e-88 259
Msa0630950 AT5G20140.1 34.031 191 103 5 11 201 206 373 5.07e-21 89.7
Msa0630950 AT3G10130.1 37.000 200 108 10 11 201 115 305 1.24e-19 85.1
Msa0630950 AT5G20140.2 33.898 177 94 5 11 187 206 359 1.68e-18 82.8

Find 37 sgRNAs with CRISPR-Local

Find 51 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGACAAAGATGGTGGGTTTA+AGG 0.180691 4_3:+13478408 Msa0630950:CDS
GAAGAAGGTGGGAAGAAATA+TGG 0.237445 4_3:+13478670 Msa0630950:CDS
TTGTCTCCTTTGAATGTTGA+TGG 0.274828 4_3:-13478391 None:intergenic
AAATATGGGGTGGTGACTTT+TGG 0.302611 4_3:+13478685 Msa0630950:CDS
TTCTTCTCATTTGTTACAAC+TGG 0.303094 4_3:-13478556 None:intergenic
CGATTACAATTCCAATGTTT+AGG 0.308020 4_3:+13478824 Msa0630950:CDS
TTTGATATGGGTTTAGCATT+TGG 0.339119 4_3:+13478265 None:intergenic
ATCATCATGGTTCATTCAAC+AGG 0.393338 4_3:-13478865 None:intergenic
AAGAAGGTGGGAAGAAATAT+GGG 0.405616 4_3:+13478671 Msa0630950:CDS
GGAGAAGTTGAGGTTGTGTT+TGG 0.422907 4_3:+13478744 Msa0630950:CDS
ATCACAACCCTCTCATCAAT+AGG 0.466452 4_3:-13478643 None:intergenic
CTCATTTGTTACAACTGGTA+CGG 0.490347 4_3:-13478551 None:intergenic
AGGAGAAGGTGGAGAAGTTG+AGG 0.492065 4_3:+13478734 Msa0630950:CDS
AGGGTTGTGATAAGAGAAGA+AGG 0.496954 4_3:+13478655 Msa0630950:CDS
TCCAATGTTTAGGACTAATG+AGG 0.513944 4_3:+13478834 Msa0630950:CDS
AGGTTGTGTTTGGAGAAAGA+TGG 0.524733 4_3:+13478754 Msa0630950:CDS
GGCAAGTGATGAAGTGGTGA+AGG 0.533494 4_3:+13478714 Msa0630950:CDS
TATGATCCATCAACATTCAA+AGG 0.533544 4_3:+13478385 Msa0630950:CDS
TTGTGATAAGAGAAGAAGGT+GGG 0.544794 4_3:+13478659 Msa0630950:CDS
AACATTGGAATTGTAATCGC+AGG 0.545246 4_3:-13478820 None:intergenic
ACCTAAGCCTATTGATGAGA+GGG 0.548573 4_3:+13478636 Msa0630950:CDS
TGAAGTGGTGAAGGAGAAGG+TGG 0.549018 4_3:+13478723 Msa0630950:CDS
ACCCTCTCATCAATAGGCTT+AGG 0.549333 4_3:-13478637 None:intergenic
ACCTCATTAGTCCTAAACAT+TGG 0.561532 4_3:-13478835 None:intergenic
ATTGGAATTGTAATCGCAGG+TGG 0.568955 4_3:-13478817 None:intergenic
TGATGAAGTGGTGAAGGAGA+AGG 0.572950 4_3:+13478720 Msa0630950:CDS
CTGATTTCAGCTACTAGAGA+AGG 0.573096 4_3:-13478361 None:intergenic
TTCAAAGGAGACAAAGATGG+TGG 0.597630 4_3:+13478400 Msa0630950:CDS
ACATTCAAAGGAGACAAAGA+TGG 0.611112 4_3:+13478397 Msa0630950:CDS
TACCTAAGCCTATTGATGAG+AGG 0.615966 4_3:+13478635 Msa0630950:CDS
AGGAGTGGCAAGTGATGAAG+TGG 0.624838 4_3:+13478708 Msa0630950:CDS
TCAAAGGAGACAAAGATGGT+GGG 0.635974 4_3:+13478401 Msa0630950:CDS
AACAAATGAGAAGAACAAGA+TGG 0.636525 4_3:+13478564 Msa0630950:CDS
GTTGTGATAAGAGAAGAAGG+TGG 0.672643 4_3:+13478658 Msa0630950:CDS
GACTTCTCCAGTTATCACAA+AGG 0.674539 4_3:+13478495 Msa0630950:CDS
AGGTGGGAAGAAATATGGGG+TGG 0.678173 4_3:+13478675 Msa0630950:CDS
AGAAGGTGGGAAGAAATATG+GGG 0.696955 4_3:+13478672 Msa0630950:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GTTTTGAGTATTTCATATTT+TGG - chr4_3:13478310-13478329 None:intergenic 20.0%
!!! AAGGTTTTAGTTGATTACAT+AGG + chr4_3:13478427-13478446 Msa0630950:CDS 25.0%
!! GTTGATTACATAGGAATTTT+TGG + chr4_3:13478436-13478455 Msa0630950:CDS 25.0%
!!! TTTTGTGTTTTTGTGTTTTG+TGG - chr4_3:13478466-13478485 None:intergenic 25.0%
!!! TCTACTTTTTCATACATAGA+AGG - chr4_3:13478610-13478629 None:intergenic 25.0%
! TTCTATGTATGAAAAAGTAG+AGG + chr4_3:13478609-13478628 Msa0630950:CDS 25.0%
!!! AAAGATGGTTTTAAGGTTAT+TGG + chr4_3:13478769-13478788 Msa0630950:CDS 25.0%
!!! GTTATTGGAGATTTTTTGTT+GGG + chr4_3:13478784-13478803 Msa0630950:CDS 25.0%
TATGATCCATCAACATTCAA+AGG + chr4_3:13478385-13478404 Msa0630950:CDS 30.0%
! TTGTTTTCCTTTGTGATAAC+TGG - chr4_3:13478505-13478524 None:intergenic 30.0%
! TTCTTCTCATTTGTTACAAC+TGG - chr4_3:13478559-13478578 None:intergenic 30.0%
AACAAATGAGAAGAACAAGA+TGG + chr4_3:13478564-13478583 Msa0630950:CDS 30.0%
!! TTTGGAGAAAGATGGTTTTA+AGG + chr4_3:13478762-13478781 Msa0630950:CDS 30.0%
!!! GGTTATTGGAGATTTTTTGT+TGG + chr4_3:13478783-13478802 Msa0630950:CDS 30.0%
CGATTACAATTCCAATGTTT+AGG + chr4_3:13478824-13478843 Msa0630950:CDS 30.0%
!!! TCGGATTACATAGTTTTGTG+TGG - chr4_3:13478332-13478351 None:intergenic 35.0%
!! CTAGAGAAGGTGCATATTTT+CGG - chr4_3:13478351-13478370 None:intergenic 35.0%
TTGTCTCCTTTGAATGTTGA+TGG - chr4_3:13478394-13478413 None:intergenic 35.0%
ACATTCAAAGGAGACAAAGA+TGG + chr4_3:13478397-13478416 Msa0630950:CDS 35.0%
! CTCATTTGTTACAACTGGTA+CGG - chr4_3:13478554-13478573 None:intergenic 35.0%
TTGTGATAAGAGAAGAAGGT+GGG + chr4_3:13478659-13478678 Msa0630950:CDS 35.0%
AAGAAGGTGGGAAGAAATAT+GGG + chr4_3:13478671-13478690 Msa0630950:CDS 35.0%
!!! TTGGAGATTTTTTGTTGGGT+AGG + chr4_3:13478788-13478807 Msa0630950:CDS 35.0%
AACATTGGAATTGTAATCGC+AGG - chr4_3:13478823-13478842 None:intergenic 35.0%
! TCCAATGTTTAGGACTAATG+AGG + chr4_3:13478834-13478853 Msa0630950:CDS 35.0%
ACCTCATTAGTCCTAAACAT+TGG - chr4_3:13478838-13478857 None:intergenic 35.0%
CTGATTTCAGCTACTAGAGA+AGG - chr4_3:13478364-13478383 None:intergenic 40.0%
TTCAAAGGAGACAAAGATGG+TGG + chr4_3:13478400-13478419 Msa0630950:CDS 40.0%
TCAAAGGAGACAAAGATGGT+GGG + chr4_3:13478401-13478420 Msa0630950:CDS 40.0%
!! AGACAAAGATGGTGGGTTTA+AGG + chr4_3:13478408-13478427 Msa0630950:CDS 40.0%
GACTTCTCCAGTTATCACAA+AGG + chr4_3:13478495-13478514 Msa0630950:CDS 40.0%
TACCTAAGCCTATTGATGAG+AGG + chr4_3:13478635-13478654 Msa0630950:CDS 40.0%
ACCTAAGCCTATTGATGAGA+GGG + chr4_3:13478636-13478655 Msa0630950:CDS 40.0%
ATCACAACCCTCTCATCAAT+AGG - chr4_3:13478646-13478665 None:intergenic 40.0%
AGGGTTGTGATAAGAGAAGA+AGG + chr4_3:13478655-13478674 Msa0630950:CDS 40.0%
GTTGTGATAAGAGAAGAAGG+TGG + chr4_3:13478658-13478677 Msa0630950:CDS 40.0%
GAAGAAGGTGGGAAGAAATA+TGG + chr4_3:13478670-13478689 Msa0630950:CDS 40.0%
AGAAGGTGGGAAGAAATATG+GGG + chr4_3:13478672-13478691 Msa0630950:CDS 40.0%
! AAATATGGGGTGGTGACTTT+TGG + chr4_3:13478685-13478704 Msa0630950:CDS 40.0%
AGGTTGTGTTTGGAGAAAGA+TGG + chr4_3:13478754-13478773 Msa0630950:CDS 40.0%
ATTGGAATTGTAATCGCAGG+TGG - chr4_3:13478820-13478839 None:intergenic 40.0%
ACCCTCTCATCAATAGGCTT+AGG - chr4_3:13478640-13478659 None:intergenic 45.0%
TGATGAAGTGGTGAAGGAGA+AGG + chr4_3:13478720-13478739 Msa0630950:CDS 45.0%
GGAGAAGTTGAGGTTGTGTT+TGG + chr4_3:13478744-13478763 Msa0630950:CDS 45.0%
AGGTGGGAAGAAATATGGGG+TGG + chr4_3:13478675-13478694 Msa0630950:CDS 50.0%
! TATGGGGTGGTGACTTTTGG+AGG + chr4_3:13478688-13478707 Msa0630950:CDS 50.0%
AGGAGTGGCAAGTGATGAAG+TGG + chr4_3:13478708-13478727 Msa0630950:CDS 50.0%
GGCAAGTGATGAAGTGGTGA+AGG + chr4_3:13478714-13478733 Msa0630950:CDS 50.0%
TGAAGTGGTGAAGGAGAAGG+TGG + chr4_3:13478723-13478742 Msa0630950:CDS 50.0%
AGGAGAAGGTGGAGAAGTTG+AGG + chr4_3:13478734-13478753 Msa0630950:CDS 50.0%
!! GGTGGTGACTTTTGGAGGAG+TGG + chr4_3:13478693-13478712 Msa0630950:CDS 55.0%
Chromosome Type Strat End Strand Name
chr4_3 gene 13478271 13478876 13478271 ID=Msa0630950;Name=Msa0630950
chr4_3 mRNA 13478271 13478876 13478271 ID=Msa0630950-mRNA-1;Parent=Msa0630950;Name=Msa0630950-mRNA-1;_AED=0.25;_eAED=0.26;_QI=0|-1|0|1|-1|1|1|0|201
chr4_3 exon 13478271 13478876 13478271 ID=Msa0630950-mRNA-1:exon:3785;Parent=Msa0630950-mRNA-1
chr4_3 CDS 13478271 13478876 13478271 ID=Msa0630950-mRNA-1:cds;Parent=Msa0630950-mRNA-1
Gene Sequence

>Msa0630950

ATGGGTTTAGCATTTGGAAGATTTAGTGCTGAGACACCAAAATATGAAATACTCAAAACCACACAAAACTATGTAATCCGAAAATATGCACCTTCTCTAGTAGCTGAAATCAGCTATGATCCATCAACATTCAAAGGAGACAAAGATGGTGGGTTTAAGGTTTTAGTTGATTACATAGGAATTTTTGGTAAACCACAAAACACAAAAACACAAAAAATTTCAATGACTTCTCCAGTTATCACAAAGGAAAACAAAAGTTCATCAGAAAAGATTGCTATGACCGTACCAGTTGTAACAAATGAGAAGAACAAGATGGTGACTATGCAGTTTACTTTGCCTTCTATGTATGAAAAAGTAGAGGAAGTACCTAAGCCTATTGATGAGAGGGTTGTGATAAGAGAAGAAGGTGGGAAGAAATATGGGGTGGTGACTTTTGGAGGAGTGGCAAGTGATGAAGTGGTGAAGGAGAAGGTGGAGAAGTTGAGGTTGTGTTTGGAGAAAGATGGTTTTAAGGTTATTGGAGATTTTTTGTTGGGTAGGTATAATCCACCTGCGATTACAATTCCAATGTTTAGGACTAATGAGGTTTTGATTCCTGTTGAATGA

Protein sequence

>Msa0630950

MGLAFGRFSAETPKYEILKTTQNYVIRKYAPSLVAEISYDPSTFKGDKDGGFKVLVDYIGIFGKPQNTKTQKISMTSPVITKENKSSSEKIAMTVPVVTNEKNKMVTMQFTLPSMYEKVEEVPKPIDERVVIREEGGKKYGVVTFGGVASDEVVKEKVEKLRLCLEKDGFKVIGDFLLGRYNPPAITIPMFRTNEVLIPVE*