Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0008330 | Msa0616910 | 0.809516 | 1.820597e-50 | -8.615850e-47 |
| Msa0008850 | Msa0616910 | 0.805125 | 1.552198e-49 | -8.615850e-47 |
| Msa0011720 | Msa0616910 | 0.806684 | 7.298227e-50 | -8.615850e-47 |
| Msa0060600 | Msa0616910 | 0.818570 | 1.829760e-52 | -8.615850e-47 |
| Msa0105340 | Msa0616910 | 0.828234 | 1.007010e-54 | -8.615850e-47 |
| Msa0616910 | Msa0617830 | 0.800665 | 1.295803e-48 | -8.615850e-47 |
| Msa0616910 | Msa0626230 | 0.843029 | 1.800846e-58 | -8.615850e-47 |
| Msa0616910 | Msa0640380 | 0.800314 | 1.527829e-48 | -8.615850e-47 |
| Msa0616910 | Msa0697140 | 0.814137 | 1.795238e-51 | -8.615850e-47 |
| Msa0616910 | Msa0700070 | 0.805003 | 1.646495e-49 | -8.615850e-47 |
| Msa0616910 | Msa0701780 | 0.824115 | 9.614641e-54 | -8.615850e-47 |
| Msa0616910 | Msa0714340 | 0.824184 | 9.261146e-54 | -8.615850e-47 |
| Msa0616910 | Msa0714380 | 0.826493 | 2.632477e-54 | -8.615850e-47 |
| Msa0616910 | Msa0722930 | 0.826887 | 2.119685e-54 | -8.615850e-47 |
| Msa0616910 | Msa0723140 | 0.833039 | 6.705886e-56 | -8.615850e-47 |
| Msa0616910 | Msa0766100 | -0.803194 | 3.916675e-49 | -8.615850e-47 |
| Msa0616910 | Msa0796460 | 0.801199 | 1.007675e-48 | -8.615850e-47 |
| Msa0616910 | Msa0807050 | 0.821941 | 3.089339e-53 | -8.615850e-47 |
| Msa0616910 | Msa0832990 | 0.821846 | 3.248903e-53 | -8.615850e-47 |
| Msa0616910 | Msa0843280 | 0.822165 | 2.741312e-53 | -8.615850e-47 |
| Msa0616910 | Msa0860270 | 0.801325 | 9.496420e-49 | -8.615850e-47 |
| Msa0616910 | Msa0896210 | 0.807453 | 5.016527e-50 | -8.615850e-47 |
| Msa0616910 | Msa0988500 | 0.810424 | 1.160584e-50 | -8.615850e-47 |
| Msa0616910 | Msa1063180 | 0.800226 | 1.591634e-48 | -8.615850e-47 |
| Msa0616910 | Msa1063490 | 0.808167 | 3.537554e-50 | -8.615850e-47 |
| Msa0616910 | Msa1080770 | 0.839717 | 1.341051e-57 | -8.615850e-47 |
| Msa0616910 | Msa1080790 | 0.820449 | 6.817636e-53 | -8.615850e-47 |
| Msa0616910 | Msa1089530 | 0.817192 | 3.745512e-52 | -8.615850e-47 |
| Msa0616910 | Msa1108780 | 0.803074 | 4.145816e-49 | -8.615850e-47 |
| Msa0616910 | Msa1116140 | 0.802329 | 5.907257e-49 | -8.615850e-47 |
| Msa0616910 | Msa1116150 | 0.801922 | 7.164424e-49 | -8.615850e-47 |
| Msa0616910 | Msa1118910 | 0.801632 | 8.216602e-49 | -8.615850e-47 |
| Msa0616910 | Msa1118930 | 0.822508 | 2.282269e-53 | -8.615850e-47 |
| Msa0616910 | Msa1123340 | 0.801261 | 9.789820e-49 | -8.615850e-47 |
| Msa0616910 | Msa1132950 | 0.822246 | 2.625467e-53 | -8.615850e-47 |
| Msa0616910 | Msa1198450 | 0.812091 | 5.046927e-51 | -8.615850e-47 |
| Msa0616910 | Msa1208810 | 0.832214 | 1.074296e-55 | -8.615850e-47 |
| Msa0616910 | Msa1217810 | 0.808746 | 2.662417e-50 | -8.615850e-47 |
| Msa0616910 | Msa1244830 | 0.823252 | 1.530918e-53 | -8.615850e-47 |
| Msa0616910 | Msa1259010 | 0.820164 | 7.928258e-53 | -8.615850e-47 |
| Msa0616910 | Msa1292730 | 0.826776 | 2.252940e-54 | -8.615850e-47 |
| Msa0616910 | Msa1303720 | 0.856742 | 2.618975e-62 | -8.615850e-47 |
| Msa0616910 | Msa1303780 | 0.847665 | 1.001801e-59 | -8.615850e-47 |
| Msa0616910 | Msa1314330 | 0.804269 | 2.342189e-49 | -8.615850e-47 |
| Msa0616910 | Msa1323060 | 0.813515 | 2.461489e-51 | -8.615850e-47 |
| Msa0616910 | Msa1347620 | 0.802508 | 5.426451e-49 | -8.615850e-47 |
| Msa0616910 | Msa1355260 | 0.800216 | 1.599036e-48 | -8.615850e-47 |
| Msa0616910 | Msa1363120 | 0.821249 | 4.463372e-53 | -8.615850e-47 |
| Msa0616910 | Msa1370650 | 0.825403 | 4.777136e-54 | -8.615850e-47 |
| Msa0616910 | Msa1370910 | -0.819846 | 9.371388e-53 | -8.615850e-47 |
| Msa0616910 | Msa1382720 | 0.835269 | 1.851063e-56 | -8.615850e-47 |
| Msa0616910 | Msa1387090 | 0.811589 | 6.491155e-51 | -8.615850e-47 |
| Msa0616910 | Msa1396090 | 0.876672 | 1.159340e-68 | -8.615850e-47 |
| Msa0616910 | Msa1457670 | -0.806059 | 9.885042e-50 | -8.615850e-47 |
| Msa0151690 | Msa0616910 | 0.830728 | 2.493249e-55 | -8.615850e-47 |
| Msa0164360 | Msa0616910 | 0.821297 | 4.351356e-53 | -8.615850e-47 |
| Msa0205400 | Msa0616910 | 0.819669 | 1.028428e-52 | -8.615850e-47 |
| Msa0368370 | Msa0616910 | 0.822395 | 2.423324e-53 | -8.615850e-47 |
| Msa0378340 | Msa0616910 | 0.847234 | 1.315847e-59 | -8.615850e-47 |
| Msa0427050 | Msa0616910 | 0.807011 | 6.223937e-50 | -8.615850e-47 |
| Msa0427690 | Msa0616910 | 0.847197 | 1.346555e-59 | -8.615850e-47 |
| Msa0429070 | Msa0616910 | -0.811766 | 5.940355e-51 | -8.615850e-47 |
| Msa0479740 | Msa0616910 | 0.808398 | 3.158311e-50 | -8.615850e-47 |
| Msa0480210 | Msa0616910 | 0.819792 | 9.642335e-53 | -8.615850e-47 |
| Msa0248600 | Msa0616910 | 0.826509 | 2.608483e-54 | -8.615850e-47 |
| Msa0282500 | Msa0616910 | 0.813399 | 2.611213e-51 | -8.615850e-47 |
| Msa0286860 | Msa0616910 | 0.800879 | 1.171726e-48 | -8.615850e-47 |
| Msa0286880 | Msa0616910 | 0.817096 | 3.935942e-52 | -8.615850e-47 |
| Msa0325510 | Msa0616910 | 0.807729 | 4.384234e-50 | -8.615850e-47 |
| Msa0508140 | Msa0616910 | 0.805370 | 1.379287e-49 | -8.615850e-47 |
| Msa0518520 | Msa0616910 | 0.846848 | 1.677987e-59 | -8.615850e-47 |
| Msa0585930 | Msa0616910 | 0.808829 | 2.555355e-50 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 39 sgRNAs with CRISPR-Local
Find 50 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTATTGATTGCGGTGTATT+TGG | 0.202554 | 4_2:-67160601 | Msa0616910:three_prime_UTR |
| ATGTGGAGTGTATTAATATA+AGG | 0.293501 | 4_2:-67160932 | Msa0616910:exon |
| CTCATGAGGCTGTGAGATTA+AGG | 0.311797 | 4_2:-67160745 | Msa0616910:intron |
| TCCGGATATTCTTTGATTCC+AGG | 0.314868 | 4_2:-67160815 | Msa0616910:three_prime_UTR |
| ACCAAGAGTAACGCTATAAA+TGG | 0.318021 | 4_2:+67160779 | None:intergenic |
| CTTCCCGGACCCTGCGTATG+TGG | 0.354687 | 4_2:+67160967 | None:intergenic |
| TGTGGAGTGTATTAATATAA+GGG | 0.391175 | 4_2:-67160931 | Msa0616910:exon |
| CATATGGTGGGACCTCTTCC+CGG | 0.412378 | 4_2:+67160952 | None:intergenic |
| GCTCCCACATACGCAGGGTC+CGG | 0.423475 | 4_2:-67160971 | Msa0616910:five_prime_UTR |
| ACCTGGAATCAAAGAATATC+CGG | 0.438542 | 4_2:+67160814 | None:intergenic |
| ATATTAATACACTCCACATA+TGG | 0.458435 | 4_2:+67160936 | None:intergenic |
| ATGTGGGAGCTTTAGTGCAC+CGG | 0.460942 | 4_2:+67160984 | None:intergenic |
| ACATTTCTCAAGAACAAGCT+TGG | 0.467961 | 4_2:-67160571 | Msa0616910:three_prime_UTR |
| CATACGCAGGGTCCGGGAAG+AGG | 0.474181 | 4_2:-67160964 | Msa0616910:five_prime_UTR |
| GCCATTTATAGCGTTACTCT+TGG | 0.480930 | 4_2:-67160780 | Msa0616910:three_prime_UTR |
| TTCAATTGTTTCCACCTTAG+CGG | 0.484293 | 4_2:-67160529 | Msa0616910:three_prime_UTR |
| CTCCCACATACGCAGGGTCC+GGG | 0.492380 | 4_2:-67160970 | Msa0616910:five_prime_UTR |
| TTCCCGGACCCTGCGTATGT+GGG | 0.495793 | 4_2:+67160968 | None:intergenic |
| CGTGATTTCTTTATTGATTG+CGG | 0.531142 | 4_2:-67160610 | Msa0616910:three_prime_UTR |
| GTTTCCACCTTAGCGGTCAT+CGG | 0.533114 | 4_2:-67160522 | Msa0616910:three_prime_UTR |
| GGAAGAGGTCCCACCATATG+TGG | 0.535887 | 4_2:-67160949 | Msa0616910:exon |
| CGCAATCAATAAAGAAATCA+CGG | 0.554346 | 4_2:+67160611 | None:intergenic |
| AATGGCAACTCAAGTTCACC+TGG | 0.564932 | 4_2:+67160797 | None:intergenic |
| TGTGGGAGCTTTAGTGCACC+GGG | 0.584269 | 4_2:+67160985 | None:intergenic |
| TTAATACACTCCACATATGG+TGG | 0.587420 | 4_2:+67160939 | None:intergenic |
| TTTGCCAGGGACACAAAAGA+AGG | 0.594762 | 4_2:-67160636 | Msa0616910:intron |
| GTGGAGTGTATTAATATAAG+GGG | 0.596608 | 4_2:-67160930 | Msa0616910:exon |
| GATACCGATGACCGCTAAGG+TGG | 0.607165 | 4_2:+67160518 | None:intergenic |
| ACTAAAGCTCCCACATACGC+AGG | 0.607397 | 4_2:-67160977 | Msa0616910:five_prime_UTR |
| CTTAATCTCACAGCCTCATG+AGG | 0.615359 | 4_2:+67160746 | None:intergenic |
| TAATACACTCCACATATGGT+GGG | 0.622366 | 4_2:+67160940 | None:intergenic |
| TTGGAGAAATCATTTGACCA+AGG | 0.622670 | 4_2:-67160552 | Msa0616910:three_prime_UTR |
| ATTAATATAAGGGGCAACTG+TGG | 0.634727 | 4_2:-67160921 | Msa0616910:exon |
| AGTGATACCGATGACCGCTA+AGG | 0.637419 | 4_2:+67160515 | None:intergenic |
| AACTCAGATGAGAAGCAACA+AGG | 0.646149 | 4_2:+67160485 | None:intergenic |
| AGGTGGAAACAATTGAACCT+TGG | 0.646271 | 4_2:+67160535 | None:intergenic |
| GGTGATTGTAGCTCCTCATG+AGG | 0.668319 | 4_2:-67160759 | Msa0616910:three_prime_UTR |
| CTAAAGCTCCCACATACGCA+GGG | 0.669615 | 4_2:-67160976 | Msa0616910:five_prime_UTR |
| GTATTAATATAAGGGCAACC+CGG | 0.715868 | 4_2:-67161003 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TATTCTTTTTGAATATTAAT+TGG | - | chr4_2:67160621-67160640 | Msa0616910:three_prime_UTR | 10.0% |
| !! | TATTCAAAAAGAATAAAGAA+CGG | + | chr4_2:67160616-67160635 | None:intergenic | 15.0% |
| !! | AAAAAAGATAAAATTTCTTC+CGG | - | chr4_2:67160644-67160663 | Msa0616910:intron | 15.0% |
| ! | ATATTAATACACTCCACATA+TGG | + | chr4_2:67160544-67160563 | None:intergenic | 25.0% |
| ! | ATGTGGAGTGTATTAATATA+AGG | - | chr4_2:67160545-67160564 | Msa0616910:three_prime_UTR | 25.0% |
| ! | TGTGGAGTGTATTAATATAA+GGG | - | chr4_2:67160546-67160565 | Msa0616910:three_prime_UTR | 25.0% |
| !! | GTAAGCAAATTCATGTTTTT+GGG | - | chr4_2:67160754-67160773 | Msa0616910:three_prime_UTR | 25.0% |
| !!! | TAAGCAAATTCATGTTTTTG+GGG | - | chr4_2:67160755-67160774 | Msa0616910:three_prime_UTR | 25.0% |
| GTGGAGTGTATTAATATAAG+GGG | - | chr4_2:67160547-67160566 | Msa0616910:three_prime_UTR | 30.0% | |
| ! | GGTAAGCAAATTCATGTTTT+TGG | - | chr4_2:67160753-67160772 | Msa0616910:three_prime_UTR | 30.0% |
| ! | TAAATAGGGTAAGTTGACAA+CGG | + | chr4_2:67160789-67160808 | None:intergenic | 30.0% |
| CGCAATCAATAAAGAAATCA+CGG | + | chr4_2:67160869-67160888 | None:intergenic | 30.0% | |
| ! | CGTGATTTCTTTATTGATTG+CGG | - | chr4_2:67160867-67160886 | Msa0616910:three_prime_UTR | 30.0% |
| ! | TTTATTGATTGCGGTGTATT+TGG | - | chr4_2:67160876-67160895 | Msa0616910:three_prime_UTR | 30.0% |
| TAATACACTCCACATATGGT+GGG | + | chr4_2:67160540-67160559 | None:intergenic | 35.0% | |
| TTAATACACTCCACATATGG+TGG | + | chr4_2:67160541-67160560 | None:intergenic | 35.0% | |
| ATTAATATAAGGGGCAACTG+TGG | - | chr4_2:67160556-67160575 | Msa0616910:three_prime_UTR | 35.0% | |
| ACCTGGAATCAAAGAATATC+CGG | + | chr4_2:67160666-67160685 | None:intergenic | 35.0% | |
| ACCAAGAGTAACGCTATAAA+TGG | + | chr4_2:67160701-67160720 | None:intergenic | 35.0% | |
| ACATTTCTCAAGAACAAGCT+TGG | - | chr4_2:67160906-67160925 | Msa0616910:three_prime_UTR | 35.0% | |
| TTGGAGAAATCATTTGACCA+AGG | - | chr4_2:67160925-67160944 | Msa0616910:exon | 35.0% | |
| TTCAATTGTTTCCACCTTAG+CGG | - | chr4_2:67160948-67160967 | Msa0616910:exon | 35.0% | |
| TCCGGATATTCTTTGATTCC+AGG | - | chr4_2:67160662-67160681 | Msa0616910:intron | 40.0% | |
| GCCATTTATAGCGTTACTCT+TGG | - | chr4_2:67160697-67160716 | Msa0616910:intron | 40.0% | |
| ! | GTAAGTTGACAACGGTTCAA+AGG | + | chr4_2:67160781-67160800 | None:intergenic | 40.0% |
| ACATCGTTATTCTCTTTGCC+AGG | - | chr4_2:67160827-67160846 | Msa0616910:three_prime_UTR | 40.0% | |
| CATCGTTATTCTCTTTGCCA+GGG | - | chr4_2:67160828-67160847 | Msa0616910:three_prime_UTR | 40.0% | |
| AGGTGGAAACAATTGAACCT+TGG | + | chr4_2:67160945-67160964 | None:intergenic | 40.0% | |
| AATGGCAACTCAAGTTCACC+TGG | + | chr4_2:67160683-67160702 | None:intergenic | 45.0% | |
| CTTAATCTCACAGCCTCATG+AGG | + | chr4_2:67160734-67160753 | None:intergenic | 45.0% | |
| CTCATGAGGCTGTGAGATTA+AGG | - | chr4_2:67160732-67160751 | Msa0616910:intron | 45.0% | |
| TTTGCCAGGGACACAAAAGA+AGG | - | chr4_2:67160841-67160860 | Msa0616910:three_prime_UTR | 45.0% | |
| !!! | GTTTTTTATTTTAATAAATA+GGG | + | chr4_2:67160803-67160822 | None:intergenic | 5.0% |
| !!! | AGTTTTTTATTTTAATAAAT+AGG | + | chr4_2:67160804-67160823 | None:intergenic | 5.0% |
| ! | ATGTGGGAGCTTTAGTGCAC+CGG | + | chr4_2:67160496-67160515 | None:intergenic | 50.0% |
| ACTAAAGCTCCCACATACGC+AGG | - | chr4_2:67160500-67160519 | Msa0616910:three_prime_UTR | 50.0% | |
| CTAAAGCTCCCACATACGCA+GGG | - | chr4_2:67160501-67160520 | Msa0616910:three_prime_UTR | 50.0% | |
| GGTGATTGTAGCTCCTCATG+AGG | - | chr4_2:67160718-67160737 | Msa0616910:intron | 50.0% | |
| GTTTCCACCTTAGCGGTCAT+CGG | - | chr4_2:67160955-67160974 | Msa0616910:five_prime_UTR | 50.0% | |
| AGTGATACCGATGACCGCTA+AGG | + | chr4_2:67160965-67160984 | None:intergenic | 50.0% | |
| ! | TGTGGGAGCTTTAGTGCACC+GGG | + | chr4_2:67160495-67160514 | None:intergenic | 55.0% |
| ! | CATATGGTGGGACCTCTTCC+CGG | + | chr4_2:67160528-67160547 | None:intergenic | 55.0% |
| GGAAGAGGTCCCACCATATG+TGG | - | chr4_2:67160528-67160547 | Msa0616910:three_prime_UTR | 55.0% | |
| ! | GGCACCTTCTTTTGTGTCCC+TGG | + | chr4_2:67160848-67160867 | None:intergenic | 55.0% |
| GATACCGATGACCGCTAAGG+TGG | + | chr4_2:67160962-67160981 | None:intergenic | 55.0% | |
| TTCCCGGACCCTGCGTATGT+GGG | + | chr4_2:67160512-67160531 | None:intergenic | 60.0% | |
| GCTCCCACATACGCAGGGTC+CGG | - | chr4_2:67160506-67160525 | Msa0616910:three_prime_UTR | 65.0% | |
| CTCCCACATACGCAGGGTCC+GGG | - | chr4_2:67160507-67160526 | Msa0616910:three_prime_UTR | 65.0% | |
| CTTCCCGGACCCTGCGTATG+TGG | + | chr4_2:67160513-67160532 | None:intergenic | 65.0% | |
| CATACGCAGGGTCCGGGAAG+AGG | - | chr4_2:67160513-67160532 | Msa0616910:three_prime_UTR | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4_2 | gene | 67160488 | 67161011 | 67160488 | ID=Msa0616910;Name=Msa0616910 |
| chr4_2 | mRNA | 67160488 | 67161011 | 67160488 | ID=Msa0616910-mRNA-1;Parent=Msa0616910;Name=Msa0616910-mRNA-1;_AED=0.17;_eAED=0.17;_QI=57|1|0.5|1|1|1|2|351|6 |
| chr4_2 | exon | 67160488 | 67160650 | 67160488 | ID=Msa0616910-mRNA-1:exon:19134;Parent=Msa0616910-mRNA-1 |
| chr4_2 | exon | 67160746 | 67161011 | 67160746 | ID=Msa0616910-mRNA-1:exon:19133;Parent=Msa0616910-mRNA-1 |
| chr4_2 | five_prime_UTR | 67160955 | 67161011 | 67160955 | ID=Msa0616910-mRNA-1:five_prime_utr;Parent=Msa0616910-mRNA-1 |
| chr4_2 | CDS | 67160934 | 67160954 | 67160934 | ID=Msa0616910-mRNA-1:cds;Parent=Msa0616910-mRNA-1 |
| chr4_2 | three_prime_UTR | 67160746 | 67160933 | 67160746 | ID=Msa0616910-mRNA-1:three_prime_utr;Parent=Msa0616910-mRNA-1 |
| chr4_2 | three_prime_UTR | 67160488 | 67160650 | 67160488 | ID=Msa0616910-mRNA-1:three_prime_utr;Parent=Msa0616910-mRNA-1 |
| Gene Sequence |
| Protein sequence |
