AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0296300

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0296300 MtrunA17_Chr2g0318901 95.238 273 13 0 1 273 1 273 0.0 547
Msa0296300 MtrunA17_Chr2g0318871 82.784 273 47 0 1 273 1 273 4.49e-175 483
Msa0296300 MtrunA17_Chr2g0318931 64.312 269 87 5 1 261 5 272 3.81e-124 355
Msa0296300 MtrunA17_Chr8g0355861 45.149 268 132 7 1 260 1 261 3.82e-78 238
Msa0296300 MtrunA17_Chr8g0355321 37.540 309 129 6 10 260 1 303 3.27e-74 229
Msa0296300 MtrunA17_Chr1g0168781 42.435 271 142 6 1 264 1 264 4.62e-72 222
Msa0296300 MtrunA17_Chr8g0355871 45.918 196 97 3 71 259 29 222 1.19e-60 192
Msa0296300 MtrunA17_Chr8g0355311 49.412 170 79 2 96 265 34 196 7.22e-58 183
Msa0296300 MtrunA17_Chr8g0355841 53.165 158 73 1 111 268 362 518 1.16e-57 192
Msa0296300 MtrunA17_Chr8g0355331 48.387 155 79 1 112 266 275 428 1.36e-53 179
Msa0296300 MtrunA17_Chr8g0355331 32.407 108 65 4 1 106 1 102 4.99e-11 62.4
Msa0296300 MtrunA17_Chr8g0355831 55.333 150 66 1 111 260 204 352 1.10e-51 172
Msa0296300 MtrunA17_Chr8g0355831 29.752 121 66 3 28 133 26 142 8.91e-11 61.6
Msa0296300 MtrunA17_Chr3g0139431 31.579 323 146 11 6 259 5 321 8.44e-44 151
Msa0296300 MtrunA17_Chr1g0147261 33.663 303 149 13 1 257 1 297 1.40e-43 150
Msa0296300 MtrunA17_Chr1g0147311 29.358 327 156 10 5 258 30 354 1.00e-38 139
Msa0296300 MtrunA17_Chr1g0147331 29.846 325 152 11 5 258 4 323 2.71e-38 137
Msa0296300 MtrunA17_Chr1g0146631 29.231 325 154 11 5 258 4 323 4.52e-37 133
Msa0296300 MtrunA17_Chr1g0147241 30.671 313 158 12 1 257 1 310 3.86e-36 130
Msa0296300 MtrunA17_Chr1g0147321 32.895 228 125 9 56 257 10 235 1.06e-30 115
Msa0296300 MtrunA17_Chr1g0147291 33.184 223 121 9 62 258 45 265 1.97e-30 114
Msa0296300 MtrunA17_Chr1g0147301 33.333 228 118 10 62 258 59 283 2.58e-30 115
Msa0296300 MtrunA17_Chr1g0147271 34.579 214 111 7 73 258 171 383 3.13e-30 116
Msa0296300 MtrunA17_Chr1g0147251 31.532 222 119 6 63 257 25 240 1.23e-29 112
Msa0296300 MtrunA17_Chr3g0144421 34.752 141 83 5 128 259 162 302 5.04e-19 84.7
Msa0296300 MtrunA17_Chr4g0034821 29.778 225 116 11 73 261 75 293 4.91e-18 82.0
Msa0296300 MtrunA17_Chr3g0138231 31.250 144 89 4 128 261 125 268 2.24e-15 74.3
Msa0296300 MtrunA17_Chr4g0034731 32.867 143 84 5 129 259 290 432 1.80e-14 72.8
Msa0296300 MtrunA17_Chr3g0144451 32.639 144 87 5 128 261 134 277 1.81e-14 71.6
Msa0296300 MtrunA17_Chr4g0034721 34.266 143 82 6 129 259 434 576 3.06e-14 72.4
Msa0296300 MtrunA17_Chr4g0076231 26.897 145 95 5 123 257 13 156 6.90e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0296300 AT5G25610.1 32.780 241 131 9 48 257 149 389 4.80e-29 114
Msa0296300 AT1G49320.1 35.135 148 81 5 126 258 131 278 1.21e-16 78.2

Find 64 sgRNAs with CRISPR-Local

Find 212 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGTCATTTCCTTGGAAATA+AGG 0.195752 2_3:+49416495 Msa0296300:CDS
AAATCCTTGGGAACACTAAT+TGG 0.203495 2_3:+49416163 Msa0296300:CDS
GCAAAGATTGTGCCTCTTTA+GGG 0.226798 2_3:-49416708 None:intergenic
AGCAAAGATTGTGCCTCTTT+AGG 0.246185 2_3:-49416709 None:intergenic
ATGTTATAATATAATGAAAA+TGG 0.261104 2_3:+49416749 Msa0296300:three_prime_UTR
CGTCCCTTTGCACAACCTTA+TGG 0.298036 2_3:+49416031 Msa0296300:CDS
CACTGCCTGAAGAAGAATAT+TGG 0.311931 2_3:+49415442 Msa0296300:CDS
TCCATTGTTGATTGCAAGAA+TGG 0.315498 2_3:+49415003 Msa0296300:exon
TGGTGCCAATGTTGCTGTTT+AGG 0.323097 2_3:+49416558 Msa0296300:CDS
GCTTCCCAATATTCTTCTTC+AGG 0.355894 2_3:-49415447 None:intergenic
AAGAATATTGGGAAGCTGTT+TGG 0.356329 2_3:+49415454 Msa0296300:CDS
GCATTGTCCAAACCTAAATT+AGG 0.363667 2_3:-49416532 None:intergenic
GTTTGTCATGCTGATACTTC+AGG 0.376770 2_3:+49416415 Msa0296300:CDS
GGATTTGCAATTTCTAAACT+TGG 0.390456 2_3:+49416184 Msa0296300:CDS
AAACCTGTTTGTCATTTCCT+TGG 0.406653 2_3:+49416487 Msa0296300:CDS
GATGTGCAGCTTGCACTTGC+AGG 0.411503 2_3:+49415405 Msa0296300:intron
TATGAACATGAGCTTTATCC+AGG 0.416684 2_3:+49415980 Msa0296300:CDS
ATAGAGTCAGTGCAAAATCT+TGG 0.422753 2_3:+49416262 Msa0296300:CDS
ATGGGTACCTAATTTAGGTT+TGG 0.424424 2_3:+49416525 Msa0296300:CDS
CAATATTCTTCTTCAGGCAG+TGG 0.433179 2_3:-49415441 None:intergenic
AGAAAGCAGTGATGTGTCAT+AGG 0.434072 2_3:+49416287 Msa0296300:CDS
CTTCTTCAGGCAGTGGTGCA+TGG 0.438551 2_3:-49415434 None:intergenic
ACTGCCTGAAGAAGAATATT+GGG 0.442357 2_3:+49415443 Msa0296300:CDS
CCATTGTTGATTGCAAGAAT+GGG 0.443788 2_3:+49415004 Msa0296300:exon
TTGCGAGAACTTCTAAAGCC+TGG 0.451996 2_3:+49415501 Msa0296300:CDS
TTGTTCATGCTTATCAATGA+AGG 0.454114 2_3:-49416233 None:intergenic
TAGAAGTTCTCGCAAAGAAG+TGG 0.458067 2_3:-49415493 None:intergenic
TTTCCAAGGAAATGACAAAC+AGG 0.462441 2_3:-49416490 None:intergenic
GAAGTGGGAATGGGAGTGTT+GGG 0.463264 2_3:-49415477 None:intergenic
CTAGCTAATTAAGCTAGAGT+TGG 0.466469 2_3:+49416665 Msa0296300:three_prime_UTR
AACCTTATGGTGTTTATACA+TGG 0.471062 2_3:+49416044 Msa0296300:CDS
CAGAGAGAACTGAAATGAAA+AGG 0.473645 2_3:-49415042 None:intergenic
GTTCCATCATTTGCCACCAA+TGG 0.478916 2_3:-49416373 None:intergenic
TCATAGGTTGAACTTTCAAA+AGG 0.480588 2_3:+49416303 Msa0296300:CDS
ACACCATAAGGTTGTGCAAA+GGG 0.481077 2_3:-49416034 None:intergenic
TGCAAACAACCTTCTGGTCC+AGG 0.488692 2_3:-49415519 None:intergenic
TTAGGTTTGGACAATGCCTA+TGG 0.494021 2_3:+49416538 Msa0296300:CDS
GGAGTACTCTCCATCATGGT+TGG 0.497653 2_3:+49416579 Msa0296300:exon
ATAAAATAGCCTTATTTCCA+AGG 0.498608 2_3:-49416504 None:intergenic
ATCCATGTATAAACACCATA+AGG 0.501910 2_3:-49416046 None:intergenic
AGAAGTGGGAATGGGAGTGT+TGG 0.505103 2_3:-49415478 None:intergenic
GTTCCATTGGTGGCAAATGA+TGG 0.508291 2_3:+49416370 Msa0296300:CDS
TGAAATTGCTGACCTACCTA+TGG 0.522453 2_3:+49415922 Msa0296300:CDS
CAGATTATGAAAGCTGATCC+TGG 0.522799 2_3:+49416460 Msa0296300:CDS
TAATGAGGTATGTGTGAAGA+AGG 0.525810 2_3:+49416108 Msa0296300:CDS
TTCTCGCAAAGAAGTGGGAA+TGG 0.528027 2_3:-49415487 None:intergenic
CCCATTCTTGCAATCAACAA+TGG 0.538082 2_3:-49415004 None:intergenic
TTTAGGAGTACTCTCCATCA+TGG 0.550450 2_3:+49416575 Msa0296300:exon
GTGAAGAAGGCAGCTGCAGA+AGG 0.555483 2_3:+49416121 Msa0296300:CDS
GAACTGAAATGAAAAGGAGA+AGG 0.556891 2_3:-49415036 None:intergenic
AAATCCAATTAGTGTTCCCA+AGG 0.558642 2_3:-49416167 None:intergenic
ATTTATGTTACACAATGCCA+TGG 0.562016 2_3:-49416607 None:intergenic
AGAAGTTCTCGCAAAGAAGT+GGG 0.563836 2_3:-49415492 None:intergenic
TCTCGCAAAGAAGTGGGAAT+GGG 0.565013 2_3:-49415486 None:intergenic
AACAGCAACATTGGCACCAT+AGG 0.567015 2_3:-49416554 None:intergenic
TGTGTGTCGTCGACTTCCAT+AGG 0.575986 2_3:-49415938 None:intergenic
GTACTCCTAAACAGCAACAT+TGG 0.581094 2_3:-49416563 None:intergenic
CTTCTAAAGCCTGGACCAGA+AGG 0.582982 2_3:+49415510 Msa0296300:CDS
AGAAGGATACACATTTAATG+AGG 0.587845 2_3:+49416093 Msa0296300:CDS
TGTCGTCGACTTCCATAGGT+AGG 0.613856 2_3:-49415934 None:intergenic
TGACAAACAGGTTTACTACC+AGG 0.616609 2_3:-49416478 None:intergenic
CATGGTGTGTCCAACCATGA+TGG 0.623792 2_3:-49416589 None:intergenic
AACACCATAAGGTTGTGCAA+AGG 0.645055 2_3:-49416035 None:intergenic
CATCATGGTTGGACACACCA+TGG 0.652600 2_3:+49416590 Msa0296300:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTAAGAAACAATTTAAAATA+TGG + chr2_3:49415162-49415181 Msa0296300:intron 10.0%
!! ATGTTATAATATAATGAAAA+TGG + chr2_3:49416749-49416768 Msa0296300:three_prime_UTR 10.0%
!!! TTAAGAAACAATTTAAAATA+TGG + chr2_3:49415162-49415181 Msa0296300:intron 10.0%
!! ATGTTATAATATAATGAAAA+TGG + chr2_3:49416749-49416768 Msa0296300:three_prime_UTR 10.0%
!! TATCTTAAATTTAAAGACAT+AGG + chr2_3:49415824-49415843 Msa0296300:intron 15.0%
!!! TAAATCTAATAGAAGTTTTA+TGG + chr2_3:49415868-49415887 Msa0296300:intron 15.0%
!!! ATTCATTTTTTTTTATCAAC+AGG + chr2_3:49415896-49415915 Msa0296300:intron 15.0%
!! TATCTTAAATTTAAAGACAT+AGG + chr2_3:49415824-49415843 Msa0296300:intron 15.0%
!!! TAAATCTAATAGAAGTTTTA+TGG + chr2_3:49415868-49415887 Msa0296300:intron 15.0%
!!! ATTCATTTTTTTTTATCAAC+AGG + chr2_3:49415896-49415915 Msa0296300:intron 15.0%
!!! GCAATTAAAGTATTATTTGT+GGG + chr2_3:49415184-49415203 Msa0296300:intron 20.0%
!!! CAATTAAAGTATTATTTGTG+GGG + chr2_3:49415185-49415204 Msa0296300:intron 20.0%
!!! AATTAAAGTATTATTTGTGG+GGG + chr2_3:49415186-49415205 Msa0296300:intron 20.0%
!!! ATTTTTTTTAAGTAGTTCAG+TGG + chr2_3:49415572-49415591 Msa0296300:intron 20.0%
!! ACAAATGATGTCTTAATATA+AGG - chr2_3:49415783-49415802 None:intergenic 20.0%
!!! TTGTTTGAAACTTTTAAGAT+AGG + chr2_3:49415799-49415818 Msa0296300:intron 20.0%
!!! TGTTCATAGAAAAAAGTTTT+TGG - chr2_3:49415968-49415987 None:intergenic 20.0%
!! ATTAAAGACATTGAAAAAGA+AGG + chr2_3:49416076-49416095 Msa0296300:CDS 20.0%
!!! TTTTATTGTCATGAAATTCA+TGG + chr2_3:49416331-49416350 Msa0296300:CDS 20.0%
!! GAAAAAAAAATTCTACAATG+AGG - chr2_3:49416781-49416800 None:intergenic 20.0%
!!! GCAATTAAAGTATTATTTGT+GGG + chr2_3:49415184-49415203 Msa0296300:intron 20.0%
!!! CAATTAAAGTATTATTTGTG+GGG + chr2_3:49415185-49415204 Msa0296300:intron 20.0%
!!! AATTAAAGTATTATTTGTGG+GGG + chr2_3:49415186-49415205 Msa0296300:intron 20.0%
!!! ATTTTTTTTAAGTAGTTCAG+TGG + chr2_3:49415572-49415591 Msa0296300:intron 20.0%
!! ACAAATGATGTCTTAATATA+AGG - chr2_3:49415783-49415802 None:intergenic 20.0%
!!! TTGTTTGAAACTTTTAAGAT+AGG + chr2_3:49415799-49415818 Msa0296300:intron 20.0%
!!! TGTTCATAGAAAAAAGTTTT+TGG - chr2_3:49415968-49415987 None:intergenic 20.0%
!! ATTAAAGACATTGAAAAAGA+AGG + chr2_3:49416076-49416095 Msa0296300:CDS 20.0%
!!! TTTTATTGTCATGAAATTCA+TGG + chr2_3:49416331-49416350 Msa0296300:CDS 20.0%
!! GAAAAAAAAATTCTACAATG+AGG - chr2_3:49416781-49416800 None:intergenic 20.0%
!! GGCAATTAAAGTATTATTTG+TGG + chr2_3:49415183-49415202 Msa0296300:intron 25.0%
!! AAAATTTTGTGCAAAATCCT+TGG + chr2_3:49416150-49416169 Msa0296300:CDS 25.0%
!! AAATTTTGTGCAAAATCCTT+GGG + chr2_3:49416151-49416170 Msa0296300:CDS 25.0%
!!! ATAAAATAGCCTTATTTCCA+AGG - chr2_3:49416507-49416526 None:intergenic 25.0%
!!! TTGGAAATAAGGCTATTTTA+TGG + chr2_3:49416506-49416525 Msa0296300:CDS 25.0%
!!! TGGAAATAAGGCTATTTTAT+GGG + chr2_3:49416507-49416526 Msa0296300:CDS 25.0%
!! ATTTTATGGGTACCTAATTT+AGG + chr2_3:49416520-49416539 Msa0296300:CDS 25.0%
!! TTTCGTGAGTAAATGTTTTT+AGG + chr2_3:49416797-49416816 Msa0296300:three_prime_UTR 25.0%
!!! TTCGTGAGTAAATGTTTTTA+GGG + chr2_3:49416798-49416817 Msa0296300:three_prime_UTR 25.0%
!!! TTTTTAGGGCTAAAAATGTT+TGG + chr2_3:49416812-49416831 Msa0296300:three_prime_UTR 25.0%
!!! TTTTAGGGCTAAAAATGTTT+GGG + chr2_3:49416813-49416832 Msa0296300:three_prime_UTR 25.0%
!! GGCAATTAAAGTATTATTTG+TGG + chr2_3:49415183-49415202 Msa0296300:intron 25.0%
!! AAAATTTTGTGCAAAATCCT+TGG + chr2_3:49416150-49416169 Msa0296300:CDS 25.0%
!! AAATTTTGTGCAAAATCCTT+GGG + chr2_3:49416151-49416170 Msa0296300:CDS 25.0%
!!! ATAAAATAGCCTTATTTCCA+AGG - chr2_3:49416507-49416526 None:intergenic 25.0%
!!! TTGGAAATAAGGCTATTTTA+TGG + chr2_3:49416506-49416525 Msa0296300:CDS 25.0%
!!! TGGAAATAAGGCTATTTTAT+GGG + chr2_3:49416507-49416526 Msa0296300:CDS 25.0%
!! ATTTTATGGGTACCTAATTT+AGG + chr2_3:49416520-49416539 Msa0296300:CDS 25.0%
!! TTTCGTGAGTAAATGTTTTT+AGG + chr2_3:49416797-49416816 Msa0296300:three_prime_UTR 25.0%
!!! TTCGTGAGTAAATGTTTTTA+GGG + chr2_3:49416798-49416817 Msa0296300:three_prime_UTR 25.0%
!!! TTTTTAGGGCTAAAAATGTT+TGG + chr2_3:49416812-49416831 Msa0296300:three_prime_UTR 25.0%
!!! TTTTAGGGCTAAAAATGTTT+GGG + chr2_3:49416813-49416832 Msa0296300:three_prime_UTR 25.0%
!! AGAATTCTTAAGTGTTGAGA+GGG - chr2_3:49415107-49415126 None:intergenic 30.0%
!! AAGAATTCTTAAGTGTTGAG+AGG - chr2_3:49415108-49415127 None:intergenic 30.0%
TCTCAACACTTAAGAATTCT+TGG + chr2_3:49415107-49415126 Msa0296300:intron 30.0%
AGTTTATATAGTGCATCAGT+TGG - chr2_3:49415143-49415162 None:intergenic 30.0%
AACCTTATGGTGTTTATACA+TGG + chr2_3:49416044-49416063 Msa0296300:CDS 30.0%
ATCCATGTATAAACACCATA+AGG - chr2_3:49416049-49416068 None:intergenic 30.0%
AGAAGGATACACATTTAATG+AGG + chr2_3:49416093-49416112 Msa0296300:CDS 30.0%
GGATTTGCAATTTCTAAACT+TGG + chr2_3:49416184-49416203 Msa0296300:CDS 30.0%
TTGTTCATGCTTATCAATGA+AGG - chr2_3:49416236-49416255 None:intergenic 30.0%
! TCATAGGTTGAACTTTCAAA+AGG + chr2_3:49416303-49416322 Msa0296300:CDS 30.0%
! AAATGATGGAACAAAAACTC+AGG + chr2_3:49416384-49416403 Msa0296300:CDS 30.0%
! TTGTCATTTCCTTGGAAATA+AGG + chr2_3:49416495-49416514 Msa0296300:CDS 30.0%
ATTTATGTTACACAATGCCA+TGG - chr2_3:49416610-49416629 None:intergenic 30.0%
! TACTTTTATGTACCCTAAAG+AGG + chr2_3:49416696-49416715 Msa0296300:three_prime_UTR 30.0%
!! AGAATTCTTAAGTGTTGAGA+GGG - chr2_3:49415107-49415126 None:intergenic 30.0%
!! AAGAATTCTTAAGTGTTGAG+AGG - chr2_3:49415108-49415127 None:intergenic 30.0%
TCTCAACACTTAAGAATTCT+TGG + chr2_3:49415107-49415126 Msa0296300:intron 30.0%
AGTTTATATAGTGCATCAGT+TGG - chr2_3:49415143-49415162 None:intergenic 30.0%
AACCTTATGGTGTTTATACA+TGG + chr2_3:49416044-49416063 Msa0296300:CDS 30.0%
ATCCATGTATAAACACCATA+AGG - chr2_3:49416049-49416068 None:intergenic 30.0%
AGAAGGATACACATTTAATG+AGG + chr2_3:49416093-49416112 Msa0296300:CDS 30.0%
GGATTTGCAATTTCTAAACT+TGG + chr2_3:49416184-49416203 Msa0296300:CDS 30.0%
TTGTTCATGCTTATCAATGA+AGG - chr2_3:49416236-49416255 None:intergenic 30.0%
! TCATAGGTTGAACTTTCAAA+AGG + chr2_3:49416303-49416322 Msa0296300:CDS 30.0%
! AAATGATGGAACAAAAACTC+AGG + chr2_3:49416384-49416403 Msa0296300:CDS 30.0%
! TTGTCATTTCCTTGGAAATA+AGG + chr2_3:49416495-49416514 Msa0296300:CDS 30.0%
ATTTATGTTACACAATGCCA+TGG - chr2_3:49416610-49416629 None:intergenic 30.0%
! TACTTTTATGTACCCTAAAG+AGG + chr2_3:49416696-49416715 Msa0296300:three_prime_UTR 30.0%
! TCCATTGTTGATTGCAAGAA+TGG + chr2_3:49415003-49415022 Msa0296300:exon 35.0%
! CCATTGTTGATTGCAAGAAT+GGG + chr2_3:49415004-49415023 Msa0296300:exon 35.0%
GAACTGAAATGAAAAGGAGA+AGG - chr2_3:49415039-49415058 None:intergenic 35.0%
CAGAGAGAACTGAAATGAAA+AGG - chr2_3:49415045-49415064 None:intergenic 35.0%
! AAAGTATTATTTGTGGGGGT+TGG + chr2_3:49415190-49415209 Msa0296300:intron 35.0%
ACTGCCTGAAGAAGAATATT+GGG + chr2_3:49415443-49415462 Msa0296300:CDS 35.0%
AAGAATATTGGGAAGCTGTT+TGG + chr2_3:49415454-49415473 Msa0296300:CDS 35.0%
ATGTAATGCAAACAACCTTC+TGG - chr2_3:49415528-49415547 None:intergenic 35.0%
CTTTATGAGTATAACTCAGC+TGG - chr2_3:49415671-49415690 None:intergenic 35.0%
! TATGAACATGAGCTTTATCC+AGG + chr2_3:49415980-49415999 Msa0296300:CDS 35.0%
!! TGCACTTTCATTGTTTTTCC+TGG - chr2_3:49416001-49416020 None:intergenic 35.0%
TAATGAGGTATGTGTGAAGA+AGG + chr2_3:49416108-49416127 Msa0296300:CDS 35.0%
AAATCCTTGGGAACACTAAT+TGG + chr2_3:49416163-49416182 Msa0296300:CDS 35.0%
AAATCCAATTAGTGTTCCCA+AGG - chr2_3:49416170-49416189 None:intergenic 35.0%
ATAGAGTCAGTGCAAAATCT+TGG + chr2_3:49416262-49416281 Msa0296300:CDS 35.0%
! TCATGGAACAACAGCTTTTA+TGG + chr2_3:49416348-49416367 Msa0296300:CDS 35.0%
! AACAGCTTTTATGGTTCCAT+TGG + chr2_3:49416357-49416376 Msa0296300:CDS 35.0%
! AAACCTGTTTGTCATTTCCT+TGG + chr2_3:49416487-49416506 Msa0296300:CDS 35.0%
TTTCCAAGGAAATGACAAAC+AGG - chr2_3:49416493-49416512 None:intergenic 35.0%
! ATGGGTACCTAATTTAGGTT+TGG + chr2_3:49416525-49416544 Msa0296300:CDS 35.0%
GCATTGTCCAAACCTAAATT+AGG - chr2_3:49416535-49416554 None:intergenic 35.0%
CTAGCTAATTAAGCTAGAGT+TGG + chr2_3:49416665-49416684 Msa0296300:three_prime_UTR 35.0%
! TCCATTGTTGATTGCAAGAA+TGG + chr2_3:49415003-49415022 Msa0296300:exon 35.0%
! CCATTGTTGATTGCAAGAAT+GGG + chr2_3:49415004-49415023 Msa0296300:exon 35.0%
GAACTGAAATGAAAAGGAGA+AGG - chr2_3:49415039-49415058 None:intergenic 35.0%
CAGAGAGAACTGAAATGAAA+AGG - chr2_3:49415045-49415064 None:intergenic 35.0%
! AAAGTATTATTTGTGGGGGT+TGG + chr2_3:49415190-49415209 Msa0296300:intron 35.0%
ACTGCCTGAAGAAGAATATT+GGG + chr2_3:49415443-49415462 Msa0296300:CDS 35.0%
AAGAATATTGGGAAGCTGTT+TGG + chr2_3:49415454-49415473 Msa0296300:CDS 35.0%
ATGTAATGCAAACAACCTTC+TGG - chr2_3:49415528-49415547 None:intergenic 35.0%
CTTTATGAGTATAACTCAGC+TGG - chr2_3:49415671-49415690 None:intergenic 35.0%
! TATGAACATGAGCTTTATCC+AGG + chr2_3:49415980-49415999 Msa0296300:CDS 35.0%
!! TGCACTTTCATTGTTTTTCC+TGG - chr2_3:49416001-49416020 None:intergenic 35.0%
TAATGAGGTATGTGTGAAGA+AGG + chr2_3:49416108-49416127 Msa0296300:CDS 35.0%
AAATCCTTGGGAACACTAAT+TGG + chr2_3:49416163-49416182 Msa0296300:CDS 35.0%
AAATCCAATTAGTGTTCCCA+AGG - chr2_3:49416170-49416189 None:intergenic 35.0%
ATAGAGTCAGTGCAAAATCT+TGG + chr2_3:49416262-49416281 Msa0296300:CDS 35.0%
! TCATGGAACAACAGCTTTTA+TGG + chr2_3:49416348-49416367 Msa0296300:CDS 35.0%
! AACAGCTTTTATGGTTCCAT+TGG + chr2_3:49416357-49416376 Msa0296300:CDS 35.0%
! AAACCTGTTTGTCATTTCCT+TGG + chr2_3:49416487-49416506 Msa0296300:CDS 35.0%
TTTCCAAGGAAATGACAAAC+AGG - chr2_3:49416493-49416512 None:intergenic 35.0%
! ATGGGTACCTAATTTAGGTT+TGG + chr2_3:49416525-49416544 Msa0296300:CDS 35.0%
GCATTGTCCAAACCTAAATT+AGG - chr2_3:49416535-49416554 None:intergenic 35.0%
CTAGCTAATTAAGCTAGAGT+TGG + chr2_3:49416665-49416684 Msa0296300:three_prime_UTR 35.0%
CCCATTCTTGCAATCAACAA+TGG - chr2_3:49415007-49415026 None:intergenic 40.0%
CAATATTCTTCTTCAGGCAG+TGG - chr2_3:49415444-49415463 None:intergenic 40.0%
CACTGCCTGAAGAAGAATAT+TGG + chr2_3:49415442-49415461 Msa0296300:CDS 40.0%
GCTTCCCAATATTCTTCTTC+AGG - chr2_3:49415450-49415469 None:intergenic 40.0%
AGAAGTTCTCGCAAAGAAGT+GGG - chr2_3:49415495-49415514 None:intergenic 40.0%
TAGAAGTTCTCGCAAAGAAG+TGG - chr2_3:49415496-49415515 None:intergenic 40.0%
TGAGTATAACTCAGCTGGTA+GGG - chr2_3:49415666-49415685 None:intergenic 40.0%
ATGAGTATAACTCAGCTGGT+AGG - chr2_3:49415667-49415686 None:intergenic 40.0%
TGAAATTGCTGACCTACCTA+TGG + chr2_3:49415922-49415941 Msa0296300:CDS 40.0%
ACACCATAAGGTTGTGCAAA+GGG - chr2_3:49416037-49416056 None:intergenic 40.0%
AACACCATAAGGTTGTGCAA+AGG - chr2_3:49416038-49416057 None:intergenic 40.0%
AGAAAGCAGTGATGTGTCAT+AGG + chr2_3:49416287-49416306 Msa0296300:CDS 40.0%
! AGCTTTTATGGTTCCATTGG+TGG + chr2_3:49416360-49416379 Msa0296300:CDS 40.0%
GTTTGTCATGCTGATACTTC+AGG + chr2_3:49416415-49416434 Msa0296300:CDS 40.0%
CAGATTATGAAAGCTGATCC+TGG + chr2_3:49416460-49416479 Msa0296300:CDS 40.0%
TGACAAACAGGTTTACTACC+AGG - chr2_3:49416481-49416500 None:intergenic 40.0%
TTAGGTTTGGACAATGCCTA+TGG + chr2_3:49416538-49416557 Msa0296300:CDS 40.0%
GTACTCCTAAACAGCAACAT+TGG - chr2_3:49416566-49416585 None:intergenic 40.0%
!! TTTAGGAGTACTCTCCATCA+TGG + chr2_3:49416575-49416594 Msa0296300:exon 40.0%
GCAAAGATTGTGCCTCTTTA+GGG - chr2_3:49416711-49416730 None:intergenic 40.0%
AGCAAAGATTGTGCCTCTTT+AGG - chr2_3:49416712-49416731 None:intergenic 40.0%
CCCATTCTTGCAATCAACAA+TGG - chr2_3:49415007-49415026 None:intergenic 40.0%
CAATATTCTTCTTCAGGCAG+TGG - chr2_3:49415444-49415463 None:intergenic 40.0%
CACTGCCTGAAGAAGAATAT+TGG + chr2_3:49415442-49415461 Msa0296300:CDS 40.0%
GCTTCCCAATATTCTTCTTC+AGG - chr2_3:49415450-49415469 None:intergenic 40.0%
AGAAGTTCTCGCAAAGAAGT+GGG - chr2_3:49415495-49415514 None:intergenic 40.0%
TAGAAGTTCTCGCAAAGAAG+TGG - chr2_3:49415496-49415515 None:intergenic 40.0%
TGAGTATAACTCAGCTGGTA+GGG - chr2_3:49415666-49415685 None:intergenic 40.0%
ATGAGTATAACTCAGCTGGT+AGG - chr2_3:49415667-49415686 None:intergenic 40.0%
TGAAATTGCTGACCTACCTA+TGG + chr2_3:49415922-49415941 Msa0296300:CDS 40.0%
ACACCATAAGGTTGTGCAAA+GGG - chr2_3:49416037-49416056 None:intergenic 40.0%
AACACCATAAGGTTGTGCAA+AGG - chr2_3:49416038-49416057 None:intergenic 40.0%
AGAAAGCAGTGATGTGTCAT+AGG + chr2_3:49416287-49416306 Msa0296300:CDS 40.0%
! AGCTTTTATGGTTCCATTGG+TGG + chr2_3:49416360-49416379 Msa0296300:CDS 40.0%
GTTTGTCATGCTGATACTTC+AGG + chr2_3:49416415-49416434 Msa0296300:CDS 40.0%
CAGATTATGAAAGCTGATCC+TGG + chr2_3:49416460-49416479 Msa0296300:CDS 40.0%
TGACAAACAGGTTTACTACC+AGG - chr2_3:49416481-49416500 None:intergenic 40.0%
TTAGGTTTGGACAATGCCTA+TGG + chr2_3:49416538-49416557 Msa0296300:CDS 40.0%
GTACTCCTAAACAGCAACAT+TGG - chr2_3:49416566-49416585 None:intergenic 40.0%
!! TTTAGGAGTACTCTCCATCA+TGG + chr2_3:49416575-49416594 Msa0296300:exon 40.0%
GCAAAGATTGTGCCTCTTTA+GGG - chr2_3:49416711-49416730 None:intergenic 40.0%
AGCAAAGATTGTGCCTCTTT+AGG - chr2_3:49416712-49416731 None:intergenic 40.0%
!! CTTAAGTGTTGAGAGGGTCA+TGG - chr2_3:49415101-49415120 None:intergenic 45.0%
TCTCGCAAAGAAGTGGGAAT+GGG - chr2_3:49415489-49415508 None:intergenic 45.0%
TTCTCGCAAAGAAGTGGGAA+TGG - chr2_3:49415490-49415509 None:intergenic 45.0%
!! TTGCGAGAACTTCTAAAGCC+TGG + chr2_3:49415501-49415520 Msa0296300:CDS 45.0%
AGAAGTCTCGAGCTCAAACA+TGG + chr2_3:49415618-49415637 Msa0296300:intron 45.0%
! GTTCCATTGGTGGCAAATGA+TGG + chr2_3:49416370-49416389 Msa0296300:CDS 45.0%
GTTCCATCATTTGCCACCAA+TGG - chr2_3:49416376-49416395 None:intergenic 45.0%
AACAGCAACATTGGCACCAT+AGG - chr2_3:49416557-49416576 None:intergenic 45.0%
!! TGGTGCCAATGTTGCTGTTT+AGG + chr2_3:49416558-49416577 Msa0296300:CDS 45.0%
!! CTTAAGTGTTGAGAGGGTCA+TGG - chr2_3:49415101-49415120 None:intergenic 45.0%
TCTCGCAAAGAAGTGGGAAT+GGG - chr2_3:49415489-49415508 None:intergenic 45.0%
TTCTCGCAAAGAAGTGGGAA+TGG - chr2_3:49415490-49415509 None:intergenic 45.0%
!! TTGCGAGAACTTCTAAAGCC+TGG + chr2_3:49415501-49415520 Msa0296300:CDS 45.0%
AGAAGTCTCGAGCTCAAACA+TGG + chr2_3:49415618-49415637 Msa0296300:intron 45.0%
! GTTCCATTGGTGGCAAATGA+TGG + chr2_3:49416370-49416389 Msa0296300:CDS 45.0%
GTTCCATCATTTGCCACCAA+TGG - chr2_3:49416376-49416395 None:intergenic 45.0%
AACAGCAACATTGGCACCAT+AGG - chr2_3:49416557-49416576 None:intergenic 45.0%
!! TGGTGCCAATGTTGCTGTTT+AGG + chr2_3:49416558-49416577 Msa0296300:CDS 45.0%
GAGAGGGTCATGGAGATGAA+AGG - chr2_3:49415091-49415110 None:intergenic 50.0%
!! GAAGTGGGAATGGGAGTGTT+GGG - chr2_3:49415480-49415499 None:intergenic 50.0%
! AGAAGTGGGAATGGGAGTGT+TGG - chr2_3:49415481-49415500 None:intergenic 50.0%
!! CTTCTAAAGCCTGGACCAGA+AGG + chr2_3:49415510-49415529 Msa0296300:CDS 50.0%
TGCAAACAACCTTCTGGTCC+AGG - chr2_3:49415522-49415541 None:intergenic 50.0%
!! TGTCGTCGACTTCCATAGGT+AGG - chr2_3:49415937-49415956 None:intergenic 50.0%
!! TGTGTGTCGTCGACTTCCAT+AGG - chr2_3:49415941-49415960 None:intergenic 50.0%
CGTCCCTTTGCACAACCTTA+TGG + chr2_3:49416031-49416050 Msa0296300:CDS 50.0%
! GGAGTACTCTCCATCATGGT+TGG + chr2_3:49416579-49416598 Msa0296300:exon 50.0%
CATGGTGTGTCCAACCATGA+TGG - chr2_3:49416592-49416611 None:intergenic 50.0%
CATCATGGTTGGACACACCA+TGG + chr2_3:49416590-49416609 Msa0296300:three_prime_UTR 50.0%
GAGAGGGTCATGGAGATGAA+AGG - chr2_3:49415091-49415110 None:intergenic 50.0%
!! GAAGTGGGAATGGGAGTGTT+GGG - chr2_3:49415480-49415499 None:intergenic 50.0%
! AGAAGTGGGAATGGGAGTGT+TGG - chr2_3:49415481-49415500 None:intergenic 50.0%
!! CTTCTAAAGCCTGGACCAGA+AGG + chr2_3:49415510-49415529 Msa0296300:CDS 50.0%
TGCAAACAACCTTCTGGTCC+AGG - chr2_3:49415522-49415541 None:intergenic 50.0%
!! TGTCGTCGACTTCCATAGGT+AGG - chr2_3:49415937-49415956 None:intergenic 50.0%
!! TGTGTGTCGTCGACTTCCAT+AGG - chr2_3:49415941-49415960 None:intergenic 50.0%
CGTCCCTTTGCACAACCTTA+TGG + chr2_3:49416031-49416050 Msa0296300:CDS 50.0%
! GGAGTACTCTCCATCATGGT+TGG + chr2_3:49416579-49416598 Msa0296300:exon 50.0%
CATGGTGTGTCCAACCATGA+TGG - chr2_3:49416592-49416611 None:intergenic 50.0%
CATCATGGTTGGACACACCA+TGG + chr2_3:49416590-49416609 Msa0296300:three_prime_UTR 50.0%
GATGTGCAGCTTGCACTTGC+AGG + chr2_3:49415405-49415424 Msa0296300:intron 55.0%
CTTCTTCAGGCAGTGGTGCA+TGG - chr2_3:49415437-49415456 None:intergenic 55.0%
GTGAAGAAGGCAGCTGCAGA+AGG + chr2_3:49416121-49416140 Msa0296300:CDS 55.0%
GATGTGCAGCTTGCACTTGC+AGG + chr2_3:49415405-49415424 Msa0296300:intron 55.0%
CTTCTTCAGGCAGTGGTGCA+TGG - chr2_3:49415437-49415456 None:intergenic 55.0%
GTGAAGAAGGCAGCTGCAGA+AGG + chr2_3:49416121-49416140 Msa0296300:CDS 55.0%
Chromosome Type Strat End Strand Name
chr2_3 gene 49414968 49416895 49414968 ID=Msa0296300;Name=Msa0296300
chr2_3 mRNA 49414968 49416895 49414968 ID=Msa0296300-mRNA-1;Parent=Msa0296300;Name=Msa0296300-mRNA-1;_AED=0.00;_eAED=0.00;_QI=54|1|1|1|1|1|3|316|273
chr2_3 exon 49414968 49415063 49414968 ID=Msa0296300-mRNA-1:exon:15544;Parent=Msa0296300-mRNA-1
chr2_3 exon 49415414 49415531 49415414 ID=Msa0296300-mRNA-1:exon:15545;Parent=Msa0296300-mRNA-1
chr2_3 exon 49415918 49416895 49415918 ID=Msa0296300-mRNA-1:exon:15546;Parent=Msa0296300-mRNA-1
chr2_3 five_prime_UTR 49414968 49415021 49414968 ID=Msa0296300-mRNA-1:five_prime_utr;Parent=Msa0296300-mRNA-1
chr2_3 CDS 49415022 49415063 49415022 ID=Msa0296300-mRNA-1:cds;Parent=Msa0296300-mRNA-1
chr2_3 CDS 49415414 49415531 49415414 ID=Msa0296300-mRNA-1:cds;Parent=Msa0296300-mRNA-1
chr2_3 CDS 49415918 49416579 49415918 ID=Msa0296300-mRNA-1:cds;Parent=Msa0296300-mRNA-1
chr2_3 three_prime_UTR 49416580 49416895 49416580 ID=Msa0296300-mRNA-1:three_prime_utr;Parent=Msa0296300-mRNA-1
Gene Sequence

>Msa0296300

ATGGGATTTCATCACCTTCTCCTTTTCATTTCAGTTCTCTCTCTTGCACTTGCAGGAGAAAGCCATGCACCACTGCCTGAAGAAGAATATTGGGAAGCTGTTTGGCCCAACACTCCCATTCCCACTTCTTTGCGAGAACTTCTAAAGCCTGGACCAGAAGGTGTTGAAATTGCTGACCTACCTATGGAAGTCGACGACACACAATATCCAAAAACTTTTTTCTATGAACATGAGCTTTATCCAGGAAAAACAATGAAAGTGCAATTCAGCAAGCGTCCCTTTGCACAACCTTATGGTGTTTATACATGGATGCGTGAAATTAAAGACATTGAAAAAGAAGGATACACATTTAATGAGGTATGTGTGAAGAAGGCAGCTGCAGAAGGAGAACAAAAATTTTGTGCAAAATCCTTGGGAACACTAATTGGATTTGCAATTTCTAAACTTGGAAAAAATATTCAAGCACTTTCAAGTTCCTTCATTGATAAGCATGAACAATACAAAATAGAGTCAGTGCAAAATCTTGGAGAGAAAGCAGTGATGTGTCATAGGTTGAACTTTCAAAAGGTTGTATTTTATTGTCATGAAATTCATGGAACAACAGCTTTTATGGTTCCATTGGTGGCAAATGATGGAACAAAAACTCAGGCACTCGCTGTTTGTCATGCTGATACTTCAGGCATGAATCATGAAATGCTGCAACAGATTATGAAAGCTGATCCTGGTAGTAAACCTGTTTGTCATTTCCTTGGAAATAAGGCTATTTTATGGGTACCTAATTTAGGTTTGGACAATGCCTATGGTGCCAATGTTGCTGTTTAG
Protein sequence

>Msa0296300

MGFHHLLLFISVLSLALAGESHAPLPEEEYWEAVWPNTPIPTSLRELLKPGPEGVEIADLPMEVDDTQYPKTFFYEHELYPGKTMKVQFSKRPFAQPYGVYTWMREIKDIEKEGYTFNEVCVKKAAAEGEQKFCAKSLGTLIGFAISKLGKNIQALSSSFIDKHEQYKIESVQNLGEKAVMCHRLNFQKVVFYCHEIHGTTAFMVPLVANDGTKTQALAVCHADTSGMNHEMLQQIMKADPGSKPVCHFLGNKAILWVPNLGLDNAYGANVAV*