AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0092350

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0092350 MtrunA17_Chr2g0322861 96.257 187 7 0 1 187 64 250 1.90e-134 382
Msa0092350 MtrunA17_Chr4g0024121 70.526 190 52 3 1 187 3 191 1.81e-93 276
Msa0092350 MtrunA17_Chr7g0268501 54.749 179 78 2 10 187 11 187 2.63e-63 199
Msa0092350 MtrunA17_Chr7g0268521 54.749 179 78 2 10 187 11 187 2.63e-63 199
Msa0092350 MtrunA17_Chr1g0157051 50.276 181 86 3 10 187 8 187 8.99e-61 192
Msa0092350 MtrunA17_Chr3g0106921 49.730 185 90 3 6 187 7 191 5.03e-59 187
Msa0092350 MtrunA17_Chr3g0129051 50.000 182 89 2 8 187 12 193 1.21e-57 184
Msa0092350 MtrunA17_Chr5g0405991 50.562 178 86 2 11 187 10 186 9.10e-57 182
Msa0092350 MtrunA17_Chr2g0320061 49.180 183 91 2 7 187 8 190 3.59e-56 180
Msa0092350 MtrunA17_Chr2g0320071 50.000 178 87 2 12 187 13 190 4.15e-56 180
Msa0092350 MtrunA17_Chr5g0432441 49.457 184 90 3 10 190 16 199 8.48e-56 179
Msa0092350 MtrunA17_Chr2g0320051 48.634 183 92 2 7 187 8 190 8.51e-56 179
Msa0092350 MtrunA17_Chr3g0128061 47.826 184 94 2 10 191 12 195 1.22e-55 179
Msa0092350 MtrunA17_Chr4g0070951 47.594 187 96 2 2 187 6 191 2.01e-55 179
Msa0092350 MtrunA17_Chr8g0371381 50.000 170 83 2 19 187 21 189 2.71e-55 178
Msa0092350 MtrunA17_Chr5g0404721 47.692 195 94 3 1 187 1 195 3.68e-55 178
Msa0092350 MtrunA17_Chr1g0188431 46.154 182 96 2 11 191 26 206 3.82e-55 178
Msa0092350 MtrunA17_Chr2g0279041 48.619 181 89 3 8 187 10 187 3.82e-55 178
Msa0092350 MtrunA17_Chr2g0292181 50.000 184 90 2 10 191 24 207 7.94e-55 178
Msa0092350 MtrunA17_Chr7g0258811 48.663 187 92 3 8 191 54 239 8.75e-55 178
Msa0092350 MtrunA17_Chr2g0292241 48.913 184 92 2 10 191 15 198 1.12e-54 177
Msa0092350 MtrunA17_Chr4g0063671 48.023 177 89 2 11 187 8 181 2.52e-54 176
Msa0092350 MtrunA17_Chr1g0213061 48.223 197 97 4 10 204 13 206 3.17e-54 176
Msa0092350 MtrunA17_Chr7g0254231 49.102 167 82 2 22 187 20 184 3.61e-54 175
Msa0092350 MtrunA17_Chr6g0460711 47.486 179 91 2 10 187 7 183 4.58e-54 175
Msa0092350 MtrunA17_Chr1g0204011 49.457 184 90 3 6 187 8 190 5.77e-54 175
Msa0092350 MtrunA17_Chr5g0402581 46.774 186 94 4 10 191 11 195 6.61e-54 174
Msa0092350 MtrunA17_Chr4g0043391 47.778 180 92 2 10 187 13 192 6.70e-54 174
Msa0092350 MtrunA17_Chr2g0292091 46.561 189 98 3 6 191 5 193 8.19e-54 175
Msa0092350 MtrunA17_Chr2g0320101 46.995 183 95 2 7 187 8 190 9.98e-54 174
Msa0092350 MtrunA17_Chr2g0291751 50.000 186 86 3 8 191 18 198 1.47e-53 174
Msa0092350 MtrunA17_Chr2g0278671 51.497 167 79 2 20 186 20 184 2.11e-53 173
Msa0092350 MtrunA17_Chr3g0114451 48.947 190 91 3 7 190 5 194 2.18e-53 173
Msa0092350 MtrunA17_Chr7g0254201 47.904 167 84 2 22 187 18 182 2.61e-53 173
Msa0092350 MtrunA17_Chr5g0402571 46.196 184 96 3 10 191 13 195 3.12e-53 173
Msa0092350 MtrunA17_Chr2g0292211 47.312 186 96 2 8 191 24 209 3.38e-53 174
Msa0092350 MtrunA17_Chr8g0372601 48.588 177 89 2 13 187 19 195 3.88e-53 173
Msa0092350 MtrunA17_Chr1g0175561 46.111 180 94 2 15 191 15 194 4.03e-53 172
Msa0092350 MtrunA17_Chr2g0292101 46.927 179 93 2 15 191 16 194 4.40e-53 173
Msa0092350 MtrunA17_Chr4g0038171 47.222 180 93 2 10 187 10 189 9.77e-53 171
Msa0092350 MtrunA17_Chr4g0068621 50.000 170 83 2 20 187 34 203 1.98e-52 171
Msa0092350 MtrunA17_Chr2g0299691 47.283 184 95 2 5 187 37 219 3.11e-52 171
Msa0092350 MtrunA17_Chr2g0320091 46.995 183 95 2 7 187 8 190 5.89e-52 169
Msa0092350 MtrunA17_Chr2g0292251 48.000 175 89 2 19 191 23 197 4.26e-51 168
Msa0092350 MtrunA17_Chr6g0477071 44.751 181 98 2 12 191 42 221 5.72e-51 167
Msa0092350 MtrunA17_Chr4g0013201 45.556 180 96 2 10 187 32 211 7.39e-51 167
Msa0092350 MtrunA17_Chr5g0400331 51.852 162 76 2 28 187 30 191 7.64e-51 167
Msa0092350 MtrunA17_Chr3g0129061 49.451 182 90 2 8 187 12 193 1.42e-50 166
Msa0092350 MtrunA17_Chr2g0292261 47.222 180 93 2 10 187 15 194 4.38e-50 166
Msa0092350 MtrunA17_Chr4g0013171 45.745 188 98 4 7 190 10 197 4.47e-50 165
Msa0092350 MtrunA17_Chr2g0292111 47.283 184 95 2 10 191 15 198 1.05e-49 164
Msa0092350 MtrunA17_Chr3g0129041 50.000 180 87 3 11 187 14 193 1.81e-49 163
Msa0092350 MtrunA17_Chr2g0320031 45.652 184 97 3 7 187 8 191 2.27e-49 163
Msa0092350 MtrunA17_Chr2g0296621 47.561 164 83 2 24 187 50 210 3.11e-49 163
Msa0092350 MtrunA17_Chr5g0432351 48.634 183 89 5 10 187 11 193 8.11e-49 161
Msa0092350 MtrunA17_Chr5g0432341 49.180 183 88 5 10 187 11 193 9.53e-49 161
Msa0092350 MtrunA17_Chr4g0038151 45.604 182 97 2 8 187 9 190 1.17e-48 161
Msa0092350 MtrunA17_Chr7g0244461 43.523 193 105 4 11 201 8 198 1.21e-48 160
Msa0092350 MtrunA17_Chr1g0155611 49.697 165 81 2 28 190 50 214 1.45e-48 161
Msa0092350 MtrunA17_Chr5g0438021 46.703 182 94 3 8 187 16 196 1.64e-48 160
Msa0092350 MtrunA17_Chr7g0244451 46.369 179 94 2 11 187 11 189 3.34e-48 160
Msa0092350 MtrunA17_Chr4g0038161 45.556 180 96 2 10 187 10 189 8.08e-48 159
Msa0092350 MtrunA17_Chr2g0292151 45.652 184 98 2 10 191 15 198 9.60e-48 159
Msa0092350 MtrunA17_Chr4g0013721 45.349 172 92 2 18 187 94 265 2.19e-47 160
Msa0092350 MtrunA17_Chr2g0292271 47.059 170 88 2 20 187 25 194 4.52e-47 158
Msa0092350 MtrunA17_Chr1g0194271 45.161 186 94 4 10 187 12 197 1.32e-46 156
Msa0092350 MtrunA17_Chr4g0076211 47.531 162 82 2 26 187 43 201 1.75e-46 155
Msa0092350 MtrunA17_Chr4g0013181 49.102 167 81 4 28 190 63 229 1.79e-46 156
Msa0092350 MtrunA17_Chr5g0432331 47.120 191 95 6 2 187 35 224 2.45e-46 156
Msa0092350 MtrunA17_Chr1g0194151 45.161 186 94 4 10 187 12 197 4.52e-46 155
Msa0092350 MtrunA17_Chr7g0225221 42.077 183 104 1 7 187 8 190 6.51e-46 154
Msa0092350 MtrunA17_Chr4g0013821 43.529 170 94 2 20 187 24 193 7.88e-46 154
Msa0092350 MtrunA17_Chr2g0316811 43.850 187 101 2 22 204 48 234 1.50e-45 153
Msa0092350 MtrunA17_Chr5g0421411 49.275 138 68 2 52 187 9 146 1.79e-45 152
Msa0092350 MtrunA17_Chr2g0292291 44.444 180 98 2 10 187 16 195 3.92e-45 153
Msa0092350 MtrunA17_Chr1g0164891 49.405 168 80 3 25 187 90 257 1.55e-44 152
Msa0092350 MtrunA17_Chr3g0107881 45.789 190 97 6 7 190 9 198 1.82e-44 150
Msa0092350 MtrunA17_Chr2g0316481 46.154 169 88 1 22 187 74 242 9.67e-44 150
Msa0092350 MtrunA17_Chr7g0224371 44.444 162 88 2 28 187 35 196 1.61e-43 148
Msa0092350 MtrunA17_Chr2g0316791 46.154 169 88 1 22 187 74 242 1.77e-43 150
Msa0092350 MtrunA17_Chr4g0046131 41.304 184 98 3 23 196 41 224 3.19e-43 147
Msa0092350 MtrunA17_Chr4g0013191 43.787 169 92 3 22 187 43 211 8.92e-43 146
Msa0092350 MtrunA17_Chr2g0316831 44.970 169 90 1 22 187 110 278 3.49e-42 147
Msa0092350 MtrunA17_Chr3g0136751 42.941 170 95 1 7 176 8 175 4.06e-42 144
Msa0092350 MtrunA17_Chr3g0106901 45.355 183 97 3 7 187 15 196 9.85e-42 143
Msa0092350 MtrunA17_Chr5g0401431 42.162 185 102 3 11 190 14 198 1.06e-41 143
Msa0092350 MtrunA17_Chr8g0364291 39.344 183 107 3 12 190 10 192 2.97e-41 142
Msa0092350 MtrunA17_Chr5g0432401 45.000 180 91 5 5 176 7 186 4.02e-41 140
Msa0092350 MtrunA17_Chr2g0316191 44.379 169 91 1 22 187 74 242 5.63e-41 143
Msa0092350 MtrunA17_Chr4g0053871 41.935 186 103 3 11 191 14 199 6.72e-41 141
Msa0092350 MtrunA17_Chr2g0311251 45.562 169 88 2 22 187 9 176 1.17e-39 138
Msa0092350 MtrunA17_Chr1g0148021 41.209 182 102 3 11 187 13 194 1.79e-39 137
Msa0092350 MtrunA17_Chr6g0467161 39.444 180 102 3 18 191 35 213 8.83e-39 136
Msa0092350 MtrunA17_Chr2g0329641 38.743 191 109 4 8 193 15 202 9.41e-38 133
Msa0092350 MtrunA17_Chr2g0311241 37.321 209 88 1 22 187 58 266 1.05e-37 135
Msa0092350 MtrunA17_Chr1g0181511 43.114 167 94 1 22 187 25 191 1.14e-37 132
Msa0092350 MtrunA17_Chr1g0197411 35.000 200 122 4 8 200 9 207 1.62e-37 132
Msa0092350 MtrunA17_Chr4g0072091 39.535 172 98 3 23 190 23 192 1.88e-36 129
Msa0092350 MtrunA17_Chr6g0467121 40.000 170 97 3 24 191 74 240 4.60e-36 130
Msa0092350 MtrunA17_Chr1g0181431 40.719 167 98 1 22 187 25 191 6.64e-36 128
Msa0092350 MtrunA17_Chr4g0075241 35.928 167 105 1 23 187 28 194 2.46e-34 124
Msa0092350 MtrunA17_Chr5g0413051 39.181 171 100 3 21 187 70 240 4.04e-34 124
Msa0092350 MtrunA17_Chr6g0467251 36.842 171 104 3 24 191 36 205 1.02e-33 123
Msa0092350 MtrunA17_Chr6g0451571 37.791 172 103 3 20 187 30 201 1.22e-33 122
Msa0092350 MtrunA17_Chr4g0074851 40.000 180 103 3 11 187 19 196 1.44e-33 122
Msa0092350 MtrunA17_Chr1g0181481 40.719 167 98 1 22 187 25 191 1.29e-32 119
Msa0092350 MtrunA17_Chr2g0279061 50.833 120 56 2 68 187 12 128 3.57e-32 117
Msa0092350 MtrunA17_Chr3g0114491 47.009 117 60 2 15 129 9 125 3.74e-28 103
Msa0092350 MtrunA17_Chr1g0181491 47.273 110 56 2 22 130 10 118 5.60e-27 100
Msa0092350 MtrunA17_Chr4g0004591 33.537 164 72 1 24 187 77 203 3.09e-26 103
Msa0092350 MtrunA17_Chr2g0316141 47.321 112 56 1 70 178 11 122 1.84e-25 99.8
Msa0092350 MtrunA17_Chr2g0316181 47.321 112 56 1 70 178 11 122 1.84e-25 99.8
Msa0092350 MtrunA17_Chr7g0254181 42.857 105 57 1 85 189 22 123 3.20e-23 90.9
Msa0092350 MtrunA17_Chr6g0468731 58.209 67 26 1 63 129 22 86 9.40e-22 85.1
Msa0092350 MtrunA17_Chr1g0196191 59.701 67 26 1 66 131 27 93 1.46e-19 81.3
Msa0092350 MtrunA17_Chr7g0254171 38.889 108 61 2 69 175 3 106 1.44e-17 75.9
Msa0092350 MtrunA17_Chr3g0114461 55.556 63 28 0 128 190 5 67 9.20e-16 72.4
Msa0092350 MtrunA17_Chr2g0292281 51.351 74 36 0 114 187 17 90 4.14e-15 71.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0092350 AT4G11290.1 67.778 180 56 1 10 187 11 190 1.53e-89 266
Msa0092350 AT3G21770.1 69.942 173 52 0 19 191 24 196 4.64e-89 265
Msa0092350 AT1G05260.1 72.455 167 46 0 21 187 23 189 2.07e-87 261
Msa0092350 AT5G15180.1 54.592 196 86 2 1 193 6 201 3.04e-72 222
Msa0092350 AT3G01190.1 53.933 178 79 2 11 187 12 187 1.70e-63 199
Msa0092350 AT1G05240.1 53.049 164 77 0 24 187 26 189 3.79e-63 199
Msa0092350 AT1G05250.1 53.049 164 77 0 24 187 26 189 3.79e-63 199
Msa0092350 AT3G03670.1 49.162 179 90 1 10 187 9 187 3.94e-60 191
Msa0092350 AT5G17820.1 49.198 187 91 2 1 187 1 183 5.78e-60 190
Msa0092350 AT5G17820.2 49.198 187 91 2 1 187 54 236 5.02e-59 189
Msa0092350 AT4G36430.1 47.917 192 97 3 13 202 19 209 9.96e-58 185
Msa0092350 AT5G42180.1 48.889 180 89 3 10 187 8 186 1.69e-57 184
Msa0092350 AT2G41480.1 45.789 190 99 3 5 193 9 195 1.46e-56 182
Msa0092350 AT2G18150.1 50.562 178 85 3 27 202 39 215 2.48e-56 182
Msa0092350 AT5G66390.1 48.936 188 94 2 8 193 17 204 3.67e-56 181
Msa0092350 AT1G77100.1 50.898 167 81 1 20 186 20 185 2.46e-55 178
Msa0092350 AT1G49570.1 48.352 182 90 3 22 199 46 227 7.13e-55 178
Msa0092350 AT4G26010.1 48.315 178 89 2 11 187 9 184 1.04e-54 177
Msa0092350 AT4G26010.2 48.315 178 89 2 11 187 37 212 2.17e-54 177
Msa0092350 AT4G33420.1 47.283 184 94 3 10 191 22 204 5.90e-54 175
Msa0092350 AT2G38390.1 48.187 193 94 5 5 191 9 201 8.80e-54 175
Msa0092350 AT5G06730.1 43.005 193 104 3 5 191 10 202 1.03e-53 175
Msa0092350 AT4G33420.2 47.283 184 94 3 10 191 69 251 2.46e-53 175
Msa0092350 AT2G38380.1 48.187 193 94 5 5 191 9 201 3.56e-53 174
Msa0092350 AT3G50990.1 47.872 188 96 2 17 202 34 221 3.61e-53 174
Msa0092350 AT5G64120.1 50.307 163 78 2 25 187 36 195 4.34e-53 173
Msa0092350 AT5G06720.1 44.022 184 101 2 10 191 18 201 5.09e-53 173
Msa0092350 AT4G25980.2 48.851 174 87 2 22 195 69 240 5.18e-53 171
Msa0092350 AT4G16270.1 50.000 166 81 2 24 187 50 215 1.19e-52 172
Msa0092350 AT5G05340.1 48.555 173 87 2 21 191 27 199 2.97e-52 171
Msa0092350 AT1G44970.1 49.425 174 86 2 16 187 37 210 3.29e-52 171
Msa0092350 AT5G51890.1 47.778 180 90 3 11 187 11 189 7.19e-52 170
Msa0092350 AT5G39580.2 46.448 183 94 3 10 192 12 190 1.60e-51 166
Msa0092350 AT2G18140.1 46.893 177 91 3 28 202 39 214 2.51e-51 169
Msa0092350 AT2G35380.1 50.307 163 79 2 27 187 33 195 9.40e-51 167
Msa0092350 AT4G25980.1 49.697 165 81 2 22 186 24 186 1.27e-50 166
Msa0092350 AT5G19890.1 47.027 185 94 3 8 191 14 195 3.50e-50 166
Msa0092350 AT2G39040.1 49.133 173 85 2 21 190 43 215 4.80e-50 166
Msa0092350 AT5G39580.1 47.191 178 90 3 10 187 12 185 4.83e-50 165
Msa0092350 AT4G08780.1 47.429 175 89 3 20 191 20 194 5.23e-50 166
Msa0092350 AT4G08770.1 46.667 180 93 3 15 191 15 194 5.46e-50 166
Msa0092350 AT5G14130.1 44.262 183 100 1 7 187 15 197 2.10e-49 164
Msa0092350 AT5G14130.2 44.262 183 100 1 7 187 56 238 4.50e-49 164
Msa0092350 AT5G58390.1 45.304 181 96 2 10 187 7 187 5.14e-49 162
Msa0092350 AT1G68850.1 44.578 166 90 2 24 187 30 195 1.08e-48 162
Msa0092350 AT3G32980.1 45.902 183 94 4 14 191 19 201 3.39e-48 161
Msa0092350 AT3G49110.1 46.739 184 93 4 11 191 22 203 5.51e-48 160
Msa0092350 AT5G58400.1 43.386 189 104 3 2 187 8 196 2.45e-47 158
Msa0092350 AT3G49120.1 46.154 182 95 3 13 191 21 202 3.18e-47 159
Msa0092350 AT2G24800.2 43.575 179 99 1 22 200 29 205 4.06e-47 155
Msa0092350 AT1G14550.1 45.946 185 95 4 8 187 8 192 1.71e-46 155
Msa0092350 AT5G22410.1 43.523 193 99 4 1 187 1 189 5.06e-45 152
Msa0092350 AT2G24800.1 45.181 166 89 1 22 187 29 192 2.88e-44 150
Msa0092350 AT1G14540.1 43.316 187 103 3 8 191 4 190 3.50e-44 150
Msa0092350 AT1G34510.1 42.553 188 103 3 1 187 1 184 4.01e-44 149
Msa0092350 AT4G33870.1 39.444 180 108 1 9 187 53 232 2.82e-43 149
Msa0092350 AT2G18980.1 41.538 195 106 4 1 190 1 192 5.63e-43 147
Msa0092350 AT5G64100.1 43.558 163 88 2 25 187 38 196 6.35e-43 147
Msa0092350 AT2G34060.1 42.614 176 96 2 17 187 35 210 7.65e-43 147
Msa0092350 AT1G71695.1 47.024 168 85 3 24 187 44 211 1.25e-42 147
Msa0092350 AT5G64110.1 40.212 189 104 3 3 186 8 192 2.51e-42 145
Msa0092350 AT4G31760.1 43.275 171 91 2 19 187 24 190 5.11e-42 144
Msa0092350 AT3G49960.1 39.086 197 115 3 11 202 14 210 7.46e-41 141
Msa0092350 AT2G22420.1 39.227 181 107 3 10 187 8 188 4.56e-40 139
Msa0092350 AT5G67400.1 40.110 182 104 3 11 187 14 195 4.17e-39 137
Msa0092350 AT4G37530.2 39.362 188 109 3 20 202 23 210 3.50e-38 133
Msa0092350 AT5G19880.1 40.860 186 106 2 10 191 11 196 4.55e-38 134
Msa0092350 AT4G37520.1 39.247 186 108 3 22 202 25 210 8.34e-38 133
Msa0092350 AT4G37530.1 39.362 188 109 3 20 202 23 210 1.21e-37 133
Msa0092350 AT4G30170.1 39.412 170 96 3 22 187 25 191 1.36e-37 133
Msa0092350 AT1G30870.1 41.176 170 97 2 24 193 51 217 3.29e-37 132
Msa0092350 AT5G40150.1 39.326 178 105 2 16 190 24 201 3.85e-37 132
Msa0092350 AT5G47000.1 37.368 190 109 4 10 190 14 202 3.77e-36 129
Msa0092350 AT4G17690.1 40.476 168 95 3 24 187 26 192 4.14e-36 129
Msa0092350 AT4G37520.2 39.247 186 105 4 22 202 25 207 4.55e-36 129
Msa0092350 AT3G17070.1 36.000 200 117 4 8 196 19 218 1.29e-35 128
Msa0092350 AT4G21960.1 39.037 187 105 2 10 187 9 195 1.76e-35 127
Msa0092350 AT3G28200.1 38.953 172 102 2 19 187 16 187 2.15e-35 127
Msa0092350 AT5G24070.1 36.548 197 105 5 6 187 5 196 3.05e-34 124
Msa0092350 AT2G43480.1 38.286 175 102 3 15 187 26 196 6.38e-34 123
Msa0092350 AT1G24110.1 36.224 196 118 4 11 200 6 200 2.21e-33 122
Msa0092350 AT2G37130.1 37.278 169 104 2 21 187 27 195 5.02e-31 115
Msa0092350 AT2G35380.2 52.381 105 49 1 84 187 3 107 2.53e-30 112
Msa0092350 AT2G37130.2 37.805 164 100 2 26 187 1 164 7.23e-30 112
Msa0092350 AT3G17070.2 35.948 153 89 4 52 196 4 155 1.37e-22 92.4
Msa0092350 AT3G42570.1 38.393 112 67 2 79 188 37 148 3.27e-17 75.5

Find 37 sgRNAs with CRISPR-Local

Find 104 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTCCAAATCTTACTGTTAG+AGG 0.255087 1_2:+74757743 Msa0092350:CDS
CTTGATTGGCAAAGAGAGTT+TGG 0.269943 1_2:-74758129 None:intergenic
ACTTTCATGATTGCTTTGTT+AGG 0.271511 1_2:+74757565 Msa0092350:CDS
GCAATGATTAGTTCAGCTTT+TGG 0.305474 1_2:-74757480 None:intergenic
AGTTTGGAGGGTGGAGAAGT+TGG 0.319139 1_2:-74758113 None:intergenic
CTTTCATGATTGCTTTGTTA+GGG 0.352765 1_2:+74757566 Msa0092350:CDS
AAAGACTTAGTCCTCCTATC+TGG 0.364479 1_2:+74758163 Msa0092350:CDS
GGAAAGTTCCAACAGGTAGA+AGG 0.373285 1_2:+74758035 Msa0092350:CDS
GATTTCAGGGTGGTCCATAT+TGG 0.381350 1_2:+74758014 Msa0092350:intron
CCTGTTGGAACTTTCCAATA+TGG 0.384950 1_2:-74758028 None:intergenic
AAGCCTCTAACAGTAAGATT+TGG 0.388544 1_2:-74757746 None:intergenic
GAGGGTGGAGAAGTTGGAAA+AGG 0.426836 1_2:-74758107 None:intergenic
AAATCAGTGTAATTACTTCA+AGG 0.442898 1_2:+74757386 Msa0092350:CDS
CAAACTCTCTTTGCCAATCA+AGG 0.450028 1_2:+74758130 Msa0092350:CDS
AGGGTGGAGAAGTTGGAAAA+GGG 0.456129 1_2:-74758106 None:intergenic
TGTGGCTGCAATAGAGTCTC+TGG 0.459357 1_2:-74757852 None:intergenic
TGGAGTCGTATCGAATTTGC+TGG 0.467430 1_2:+74758060 Msa0092350:CDS
GTTGGTTTGTTGAGTTCAGA+AGG 0.478806 1_2:-74757703 None:intergenic
GAGAAGTTGGAAAAGGGAGC+AGG 0.486453 1_2:-74758100 None:intergenic
CCTCTAACAGTAAGATTTGG+AGG 0.486565 1_2:-74757743 None:intergenic
CCATATTGGAAAGTTCCAAC+AGG 0.513974 1_2:+74758028 Msa0092350:CDS
GGCAAAGAGAGTTTGGAGGG+TGG 0.528494 1_2:-74758122 None:intergenic
TGAATGTAATAATACCAGAT+AGG 0.531919 1_2:-74758177 None:intergenic
CTTGTTGAAGCTGAATGCCC+TGG 0.535811 1_2:+74757794 Msa0092350:CDS
GACGAAAGTGCTTAATGCTA+AGG 0.539713 1_2:-74758207 None:intergenic
ACTCCATCCCTTCTACCTGT+TGG 0.553125 1_2:-74758043 None:intergenic
GAAAGTTCCAACAGGTAGAA+GGG 0.557568 1_2:+74758036 Msa0092350:CDS
GTTCCAACAGGTAGAAGGGA+TGG 0.566917 1_2:+74758040 Msa0092350:CDS
GATTGGCAAAGAGAGTTTGG+AGG 0.571052 1_2:-74758126 None:intergenic
ATTAATGCAGCAGCAAGTGA+TGG 0.574993 1_2:-74757534 None:intergenic
ATTGGCAAAGAGAGTTTGGA+GGG 0.582534 1_2:-74758125 None:intergenic
TCAGCACAAGAAACAACACC+AGG 0.606055 1_2:-74757812 None:intergenic
ACTCATGCTCAATTGCAGCT+AGG 0.609605 1_2:+74757438 Msa0092350:CDS
CAGGGCATTCAGCTTCAACA+AGG 0.628010 1_2:-74757793 None:intergenic
ATGTAATAATACCAGATAGG+AGG 0.629799 1_2:-74758174 None:intergenic
CAGCAAGTGATGGAGCATTG+TGG 0.631408 1_2:-74757524 None:intergenic
CAGCACAAGAAACAACACCA+GGG 0.760815 1_2:-74757811 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATACTTTCATTTCATTTT+TGG + chr1_2:74757615-74757634 Msa0092350:intron 15.0%
!!! TTATACTTTCATTTCATTTT+TGG + chr1_2:74757615-74757634 Msa0092350:intron 15.0%
!!! AACATTAAGCATTTTGATTT+AGG + chr1_2:74757664-74757683 Msa0092350:intron 20.0%
!!! ACATTAAGCATTTTGATTTA+GGG + chr1_2:74757665-74757684 Msa0092350:intron 20.0%
!!! AACATTAAGCATTTTGATTT+AGG + chr1_2:74757664-74757683 Msa0092350:intron 20.0%
!!! ACATTAAGCATTTTGATTTA+GGG + chr1_2:74757665-74757684 Msa0092350:intron 20.0%
! AAATCAGTGTAATTACTTCA+AGG + chr1_2:74757386-74757405 Msa0092350:CDS 25.0%
! ATTCTATCTTTAACAGACTA+AGG + chr1_2:74757886-74757905 Msa0092350:intron 25.0%
! TTAAATAAGTCAATCCAAAC+AGG - chr1_2:74757912-74757931 None:intergenic 25.0%
!! TTTTAAATCAAGTCCTTGAT+TGG - chr1_2:74758146-74758165 None:intergenic 25.0%
! TGAATGTAATAATACCAGAT+AGG - chr1_2:74758180-74758199 None:intergenic 25.0%
! AAATCAGTGTAATTACTTCA+AGG + chr1_2:74757386-74757405 Msa0092350:CDS 25.0%
! ATTCTATCTTTAACAGACTA+AGG + chr1_2:74757886-74757905 Msa0092350:intron 25.0%
! TTAAATAAGTCAATCCAAAC+AGG - chr1_2:74757912-74757931 None:intergenic 25.0%
!! TTTTAAATCAAGTCCTTGAT+TGG - chr1_2:74758146-74758165 None:intergenic 25.0%
! TGAATGTAATAATACCAGAT+AGG - chr1_2:74758180-74758199 None:intergenic 25.0%
! ACTTTCATGATTGCTTTGTT+AGG + chr1_2:74757565-74757584 Msa0092350:CDS 30.0%
! CTTTCATGATTGCTTTGTTA+GGG + chr1_2:74757566-74757585 Msa0092350:CDS 30.0%
!!! ATTTTGATTTAGGGATGTGA+TGG + chr1_2:74757674-74757693 Msa0092350:intron 30.0%
ATAGAATTGAGTACTTACTG+TGG - chr1_2:74757873-74757892 None:intergenic 30.0%
! ATTTGGTGATTTGTGATTTC+AGG + chr1_2:74758000-74758019 Msa0092350:intron 30.0%
! TTTGGTGATTTGTGATTTCA+GGG + chr1_2:74758001-74758020 Msa0092350:intron 30.0%
ATGTAATAATACCAGATAGG+AGG - chr1_2:74758177-74758196 None:intergenic 30.0%
! ACTTTCATGATTGCTTTGTT+AGG + chr1_2:74757565-74757584 Msa0092350:CDS 30.0%
! CTTTCATGATTGCTTTGTTA+GGG + chr1_2:74757566-74757585 Msa0092350:CDS 30.0%
!!! ATTTTGATTTAGGGATGTGA+TGG + chr1_2:74757674-74757693 Msa0092350:intron 30.0%
ATAGAATTGAGTACTTACTG+TGG - chr1_2:74757873-74757892 None:intergenic 30.0%
! ATTTGGTGATTTGTGATTTC+AGG + chr1_2:74758000-74758019 Msa0092350:intron 30.0%
! TTTGGTGATTTGTGATTTCA+GGG + chr1_2:74758001-74758020 Msa0092350:intron 30.0%
ATGTAATAATACCAGATAGG+AGG - chr1_2:74758177-74758196 None:intergenic 30.0%
!! GCAATGATTAGTTCAGCTTT+TGG - chr1_2:74757483-74757502 None:intergenic 35.0%
!!! GAGCATTTTTTTCAGCTTGT+TGG - chr1_2:74757724-74757743 None:intergenic 35.0%
AAGCCTCTAACAGTAAGATT+TGG - chr1_2:74757749-74757768 None:intergenic 35.0%
! CTACTGACTACTGATACATT+TGG + chr1_2:74757983-74758002 Msa0092350:intron 35.0%
!! GCAATGATTAGTTCAGCTTT+TGG - chr1_2:74757483-74757502 None:intergenic 35.0%
!!! GAGCATTTTTTTCAGCTTGT+TGG - chr1_2:74757724-74757743 None:intergenic 35.0%
AAGCCTCTAACAGTAAGATT+TGG - chr1_2:74757749-74757768 None:intergenic 35.0%
! CTACTGACTACTGATACATT+TGG + chr1_2:74757983-74758002 Msa0092350:intron 35.0%
! TTCAGCTTTTGGACAACTCT+TGG - chr1_2:74757472-74757491 None:intergenic 40.0%
ATTAATGCAGCAGCAAGTGA+TGG - chr1_2:74757537-74757556 None:intergenic 40.0%
!! GTTGGTTTGTTGAGTTCAGA+AGG - chr1_2:74757706-74757725 None:intergenic 40.0%
CCTCTAACAGTAAGATTTGG+AGG - chr1_2:74757746-74757765 None:intergenic 40.0%
CCTCCAAATCTTACTGTTAG+AGG + chr1_2:74757743-74757762 Msa0092350:CDS 40.0%
TTAACAGACTAAGGCCTGTT+TGG + chr1_2:74757895-74757914 Msa0092350:intron 40.0%
CCTGTTGGAACTTTCCAATA+TGG - chr1_2:74758031-74758050 None:intergenic 40.0%
CCATATTGGAAAGTTCCAAC+AGG + chr1_2:74758028-74758047 Msa0092350:CDS 40.0%
GAAAGTTCCAACAGGTAGAA+GGG + chr1_2:74758036-74758055 Msa0092350:CDS 40.0%
ATTGGCAAAGAGAGTTTGGA+GGG - chr1_2:74758128-74758147 None:intergenic 40.0%
CTTGATTGGCAAAGAGAGTT+TGG - chr1_2:74758132-74758151 None:intergenic 40.0%
CAAACTCTCTTTGCCAATCA+AGG + chr1_2:74758130-74758149 Msa0092350:CDS 40.0%
AAAGACTTAGTCCTCCTATC+TGG + chr1_2:74758163-74758182 Msa0092350:CDS 40.0%
!! GACGAAAGTGCTTAATGCTA+AGG - chr1_2:74758210-74758229 None:intergenic 40.0%
! TTCAGCTTTTGGACAACTCT+TGG - chr1_2:74757472-74757491 None:intergenic 40.0%
ATTAATGCAGCAGCAAGTGA+TGG - chr1_2:74757537-74757556 None:intergenic 40.0%
!! GTTGGTTTGTTGAGTTCAGA+AGG - chr1_2:74757706-74757725 None:intergenic 40.0%
CCTCTAACAGTAAGATTTGG+AGG - chr1_2:74757746-74757765 None:intergenic 40.0%
CCTCCAAATCTTACTGTTAG+AGG + chr1_2:74757743-74757762 Msa0092350:CDS 40.0%
TTAACAGACTAAGGCCTGTT+TGG + chr1_2:74757895-74757914 Msa0092350:intron 40.0%
CCTGTTGGAACTTTCCAATA+TGG - chr1_2:74758031-74758050 None:intergenic 40.0%
CCATATTGGAAAGTTCCAAC+AGG + chr1_2:74758028-74758047 Msa0092350:CDS 40.0%
GAAAGTTCCAACAGGTAGAA+GGG + chr1_2:74758036-74758055 Msa0092350:CDS 40.0%
ATTGGCAAAGAGAGTTTGGA+GGG - chr1_2:74758128-74758147 None:intergenic 40.0%
CTTGATTGGCAAAGAGAGTT+TGG - chr1_2:74758132-74758151 None:intergenic 40.0%
CAAACTCTCTTTGCCAATCA+AGG + chr1_2:74758130-74758149 Msa0092350:CDS 40.0%
AAAGACTTAGTCCTCCTATC+TGG + chr1_2:74758163-74758182 Msa0092350:CDS 40.0%
!! GACGAAAGTGCTTAATGCTA+AGG - chr1_2:74758210-74758229 None:intergenic 40.0%
ACTCATGCTCAATTGCAGCT+AGG + chr1_2:74757438-74757457 Msa0092350:CDS 45.0%
CAGCACAAGAAACAACACCA+GGG - chr1_2:74757814-74757833 None:intergenic 45.0%
TCAGCACAAGAAACAACACC+AGG - chr1_2:74757815-74757834 None:intergenic 45.0%
! GGTGATTTGTGATTTCAGGG+TGG + chr1_2:74758004-74758023 Msa0092350:intron 45.0%
GATTTCAGGGTGGTCCATAT+TGG + chr1_2:74758014-74758033 Msa0092350:intron 45.0%
GGAAAGTTCCAACAGGTAGA+AGG + chr1_2:74758035-74758054 Msa0092350:CDS 45.0%
TGGAGTCGTATCGAATTTGC+TGG + chr1_2:74758060-74758079 Msa0092350:CDS 45.0%
AGGGTGGAGAAGTTGGAAAA+GGG - chr1_2:74758109-74758128 None:intergenic 45.0%
GATTGGCAAAGAGAGTTTGG+AGG - chr1_2:74758129-74758148 None:intergenic 45.0%
ACTCATGCTCAATTGCAGCT+AGG + chr1_2:74757438-74757457 Msa0092350:CDS 45.0%
CAGCACAAGAAACAACACCA+GGG - chr1_2:74757814-74757833 None:intergenic 45.0%
TCAGCACAAGAAACAACACC+AGG - chr1_2:74757815-74757834 None:intergenic 45.0%
! GGTGATTTGTGATTTCAGGG+TGG + chr1_2:74758004-74758023 Msa0092350:intron 45.0%
GATTTCAGGGTGGTCCATAT+TGG + chr1_2:74758014-74758033 Msa0092350:intron 45.0%
GGAAAGTTCCAACAGGTAGA+AGG + chr1_2:74758035-74758054 Msa0092350:CDS 45.0%
TGGAGTCGTATCGAATTTGC+TGG + chr1_2:74758060-74758079 Msa0092350:CDS 45.0%
AGGGTGGAGAAGTTGGAAAA+GGG - chr1_2:74758109-74758128 None:intergenic 45.0%
GATTGGCAAAGAGAGTTTGG+AGG - chr1_2:74758129-74758148 None:intergenic 45.0%
!! CAGCAAGTGATGGAGCATTG+TGG - chr1_2:74757527-74757546 None:intergenic 50.0%
! CAGGGCATTCAGCTTCAACA+AGG - chr1_2:74757796-74757815 None:intergenic 50.0%
CTTGTTGAAGCTGAATGCCC+TGG + chr1_2:74757794-74757813 Msa0092350:CDS 50.0%
TGTGGCTGCAATAGAGTCTC+TGG - chr1_2:74757855-74757874 None:intergenic 50.0%
GTTCCAACAGGTAGAAGGGA+TGG + chr1_2:74758040-74758059 Msa0092350:CDS 50.0%
ACTCCATCCCTTCTACCTGT+TGG - chr1_2:74758046-74758065 None:intergenic 50.0%
GAGAAGTTGGAAAAGGGAGC+AGG - chr1_2:74758103-74758122 None:intergenic 50.0%
GAGGGTGGAGAAGTTGGAAA+AGG - chr1_2:74758110-74758129 None:intergenic 50.0%
AGTTTGGAGGGTGGAGAAGT+TGG - chr1_2:74758116-74758135 None:intergenic 50.0%
!! CAGCAAGTGATGGAGCATTG+TGG - chr1_2:74757527-74757546 None:intergenic 50.0%
! CAGGGCATTCAGCTTCAACA+AGG - chr1_2:74757796-74757815 None:intergenic 50.0%
CTTGTTGAAGCTGAATGCCC+TGG + chr1_2:74757794-74757813 Msa0092350:CDS 50.0%
TGTGGCTGCAATAGAGTCTC+TGG - chr1_2:74757855-74757874 None:intergenic 50.0%
GTTCCAACAGGTAGAAGGGA+TGG + chr1_2:74758040-74758059 Msa0092350:CDS 50.0%
ACTCCATCCCTTCTACCTGT+TGG - chr1_2:74758046-74758065 None:intergenic 50.0%
GAGAAGTTGGAAAAGGGAGC+AGG - chr1_2:74758103-74758122 None:intergenic 50.0%
GAGGGTGGAGAAGTTGGAAA+AGG - chr1_2:74758110-74758129 None:intergenic 50.0%
AGTTTGGAGGGTGGAGAAGT+TGG - chr1_2:74758116-74758135 None:intergenic 50.0%
GGCAAAGAGAGTTTGGAGGG+TGG - chr1_2:74758125-74758144 None:intergenic 55.0%
GGCAAAGAGAGTTTGGAGGG+TGG - chr1_2:74758125-74758144 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr1_2 gene 74757381 74758240 74757381 ID=Msa0092350;Name=Msa0092350
chr1_2 mRNA 74757381 74758240 74757381 ID=Msa0092350-mRNA-1;Parent=Msa0092350;Name=Msa0092350-mRNA-1;_AED=0.14;_eAED=0.14;_QI=0|0|0|1|1|1|3|0|204
chr1_2 exon 74757381 74757587 74757381 ID=Msa0092350-mRNA-1:exon:26315;Parent=Msa0092350-mRNA-1
chr1_2 exon 74757686 74757874 74757686 ID=Msa0092350-mRNA-1:exon:26316;Parent=Msa0092350-mRNA-1
chr1_2 exon 74758022 74758240 74758022 ID=Msa0092350-mRNA-1:exon:26317;Parent=Msa0092350-mRNA-1
chr1_2 CDS 74757381 74757587 74757381 ID=Msa0092350-mRNA-1:cds;Parent=Msa0092350-mRNA-1
chr1_2 CDS 74757686 74757874 74757686 ID=Msa0092350-mRNA-1:cds;Parent=Msa0092350-mRNA-1
chr1_2 CDS 74758022 74758240 74758022 ID=Msa0092350-mRNA-1:cds;Parent=Msa0092350-mRNA-1
Gene Sequence

>Msa0092350

ATGAGAAATCAGTGTAATTACTTCAAGGTTTTGATTCTTTGTATTTTAGCAGCATCAACTCATGCTCAATTGCAGCTAGGATTTTATACCAAGAGTTGTCCAAAAGCTGAACTAATCATTGCAAACTTTGTTCATGAACATATCCACAATGCTCCATCACTTGCTGCTGCATTAATAAGAATGCACTTTCATGATTGCTTTGTTAGGGGATGTGATGGATCAGTCCTTCTGAACTCAACAAACCAACAAGCTGAAAAAAATGCTCCTCCAAATCTTACTGTTAGAGGCTTTGACTTCATTGACAGAATAAAGAGCCTTGTTGAAGCTGAATGCCCTGGTGTTGTTTCTTGTGCTGATATCATAGCTTTATCTGCCAGAGACTCTATTGCAGCCACAGGTGGTCCATATTGGAAAGTTCCAACAGGTAGAAGGGATGGAGTCGTATCGAATTTGCTGGAAGCAAATCAAAACATTCCTGCTCCCTTTTCCAACTTCTCCACCCTCCAAACTCTCTTTGCCAATCAAGGACTTGATTTAAAAGACTTAGTCCTCCTATCTGGTATTATTACATTCAACAATTTCCTTAGCATTAAGCACTTTCGTCGTGCACGCTAA
Protein sequence

>Msa0092350

MRNQCNYFKVLILCILAASTHAQLQLGFYTKSCPKAELIIANFVHEHIHNAPSLAAALIRMHFHDCFVRGCDGSVLLNSTNQQAEKNAPPNLTVRGFDFIDRIKSLVEAECPGVVSCADIIALSARDSIAATGGPYWKVPTGRRDGVVSNLLEANQNIPAPFSNFSTLQTLFANQGLDLKDLVLLSGIITFNNFLSIKHFRRAR*