AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0256980

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0256980 MtrunA17_Chr2g0318901 95.238 273 13 0 1 273 1 273 0.0 547
Msa0256980 MtrunA17_Chr2g0318871 82.784 273 47 0 1 273 1 273 4.49e-175 483
Msa0256980 MtrunA17_Chr2g0318931 64.312 269 87 5 1 261 5 272 3.81e-124 355
Msa0256980 MtrunA17_Chr8g0355861 45.149 268 132 7 1 260 1 261 3.82e-78 238
Msa0256980 MtrunA17_Chr8g0355321 37.540 309 129 6 10 260 1 303 3.27e-74 229
Msa0256980 MtrunA17_Chr1g0168781 42.435 271 142 6 1 264 1 264 4.62e-72 222
Msa0256980 MtrunA17_Chr8g0355871 45.918 196 97 3 71 259 29 222 1.19e-60 192
Msa0256980 MtrunA17_Chr8g0355311 49.412 170 79 2 96 265 34 196 7.22e-58 183
Msa0256980 MtrunA17_Chr8g0355841 53.165 158 73 1 111 268 362 518 1.16e-57 192
Msa0256980 MtrunA17_Chr8g0355331 48.387 155 79 1 112 266 275 428 1.36e-53 179
Msa0256980 MtrunA17_Chr8g0355331 32.407 108 65 4 1 106 1 102 4.99e-11 62.4
Msa0256980 MtrunA17_Chr8g0355831 55.333 150 66 1 111 260 204 352 1.10e-51 172
Msa0256980 MtrunA17_Chr8g0355831 29.752 121 66 3 28 133 26 142 8.91e-11 61.6
Msa0256980 MtrunA17_Chr3g0139431 31.579 323 146 11 6 259 5 321 8.44e-44 151
Msa0256980 MtrunA17_Chr1g0147261 33.663 303 149 13 1 257 1 297 1.40e-43 150
Msa0256980 MtrunA17_Chr1g0147311 29.358 327 156 10 5 258 30 354 1.00e-38 139
Msa0256980 MtrunA17_Chr1g0147331 29.846 325 152 11 5 258 4 323 2.71e-38 137
Msa0256980 MtrunA17_Chr1g0146631 29.231 325 154 11 5 258 4 323 4.52e-37 133
Msa0256980 MtrunA17_Chr1g0147241 30.671 313 158 12 1 257 1 310 3.86e-36 130
Msa0256980 MtrunA17_Chr1g0147321 32.895 228 125 9 56 257 10 235 1.06e-30 115
Msa0256980 MtrunA17_Chr1g0147291 33.184 223 121 9 62 258 45 265 1.97e-30 114
Msa0256980 MtrunA17_Chr1g0147301 33.333 228 118 10 62 258 59 283 2.58e-30 115
Msa0256980 MtrunA17_Chr1g0147271 34.579 214 111 7 73 258 171 383 3.13e-30 116
Msa0256980 MtrunA17_Chr1g0147251 31.532 222 119 6 63 257 25 240 1.23e-29 112
Msa0256980 MtrunA17_Chr3g0144421 34.752 141 83 5 128 259 162 302 5.04e-19 84.7
Msa0256980 MtrunA17_Chr4g0034821 29.778 225 116 11 73 261 75 293 4.91e-18 82.0
Msa0256980 MtrunA17_Chr3g0138231 31.250 144 89 4 128 261 125 268 2.24e-15 74.3
Msa0256980 MtrunA17_Chr4g0034731 32.867 143 84 5 129 259 290 432 1.80e-14 72.8
Msa0256980 MtrunA17_Chr3g0144451 32.639 144 87 5 128 261 134 277 1.81e-14 71.6
Msa0256980 MtrunA17_Chr4g0034721 34.266 143 82 6 129 259 434 576 3.06e-14 72.4
Msa0256980 MtrunA17_Chr4g0076231 26.897 145 95 5 123 257 13 156 6.90e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0256980 AT5G25610.1 32.780 241 131 9 48 257 149 389 4.80e-29 114
Msa0256980 AT1G49320.1 35.135 148 81 5 126 258 131 278 1.21e-16 78.2

Find 65 sgRNAs with CRISPR-Local

Find 216 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGTCATTTCCTTGGAAATA+AGG 0.195752 2_2:+52496387 Msa0256980:CDS
AAATCCTTGGGAACACTAAT+TGG 0.203495 2_2:+52496055 Msa0256980:CDS
GCAAAGATTGTGCCTCTTTA+GGG 0.226798 2_2:-52496600 None:intergenic
AGCAAAGATTGTGCCTCTTT+AGG 0.246185 2_2:-52496601 None:intergenic
ATGTTATAATATAATGAAAA+TGG 0.261104 2_2:+52496641 Msa0256980:three_prime_UTR
CGTCCCTTTGCACAACCTTA+TGG 0.298036 2_2:+52495923 Msa0256980:CDS
CACTGCCTGAAGAAGAATAT+TGG 0.311931 2_2:+52495334 Msa0256980:CDS
TCCATTGTTGATTGCAAGAA+TGG 0.315498 2_2:+52494913 Msa0256980:exon
TGGTGCCAATGTTGCTGTTT+AGG 0.323097 2_2:+52496450 Msa0256980:CDS
ATTGAAATCAGAAAATTTAG+AGG 0.336649 2_2:+52496845 Msa0256980:three_prime_UTR
GCTTCCCAATATTCTTCTTC+AGG 0.355894 2_2:-52495339 None:intergenic
AAGAATATTGGGAAGCTGTT+TGG 0.356329 2_2:+52495346 Msa0256980:CDS
GCATTGTCCAAACCTAAATT+AGG 0.363667 2_2:-52496424 None:intergenic
GTTTGTCATGCTGATACTTC+AGG 0.376770 2_2:+52496307 Msa0256980:CDS
GGATTTGCAATTTCTAAACT+TGG 0.390456 2_2:+52496076 Msa0256980:CDS
AAACCTGTTTGTCATTTCCT+TGG 0.406653 2_2:+52496379 Msa0256980:CDS
GATGTGCAGCTTGCACTTGC+AGG 0.411503 2_2:+52495297 Msa0256980:intron
TATGAACATGAGCTTTATCC+AGG 0.416684 2_2:+52495872 Msa0256980:CDS
ATAGAGTCAGTGCAAAATCT+TGG 0.422753 2_2:+52496154 Msa0256980:CDS
ATGGGTACCTAATTTAGGTT+TGG 0.424424 2_2:+52496417 Msa0256980:CDS
CAATATTCTTCTTCAGGCAG+TGG 0.433179 2_2:-52495333 None:intergenic
AGAAAGCAGTGATGTGTCAT+AGG 0.434072 2_2:+52496179 Msa0256980:CDS
CTTCTTCAGGCAGTGGTGCA+TGG 0.438551 2_2:-52495326 None:intergenic
ACTGCCTGAAGAAGAATATT+GGG 0.442357 2_2:+52495335 Msa0256980:CDS
CCATTGTTGATTGCAAGAAT+GGG 0.443788 2_2:+52494914 Msa0256980:exon
TTGCGAGAACTTCTAAAGCC+TGG 0.451996 2_2:+52495393 Msa0256980:CDS
TTGTTCATGCTTATCAATGA+AGG 0.454114 2_2:-52496125 None:intergenic
TAGAAGTTCTCGCAAAGAAG+TGG 0.458067 2_2:-52495385 None:intergenic
TTTCCAAGGAAATGACAAAC+AGG 0.462441 2_2:-52496382 None:intergenic
GAAGTGGGAATGGGAGTGTT+GGG 0.463264 2_2:-52495369 None:intergenic
CTAGCTAATTAAGCTAGAGT+TGG 0.466469 2_2:+52496557 Msa0256980:three_prime_UTR
AACCTTATGGTGTTTATACA+TGG 0.471062 2_2:+52495936 Msa0256980:CDS
CAGAGAGAACTGAAATGAAA+AGG 0.473645 2_2:-52494952 None:intergenic
GTTCCATCATTTGCCACCAA+TGG 0.478916 2_2:-52496265 None:intergenic
TCATAGGTTGAACTTTCAAA+AGG 0.480588 2_2:+52496195 Msa0256980:CDS
ACACCATAAGGTTGTGCAAA+GGG 0.481077 2_2:-52495926 None:intergenic
TGCAAACAACCTTCTGGTCC+AGG 0.488692 2_2:-52495411 None:intergenic
TTAGGTTTGGACAATGCCTA+TGG 0.494021 2_2:+52496430 Msa0256980:CDS
GGAGTACTCTCCATCATGGT+TGG 0.497653 2_2:+52496471 Msa0256980:exon
ATAAAATAGCCTTATTTCCA+AGG 0.498608 2_2:-52496396 None:intergenic
ATCCATGTATAAACACCATA+AGG 0.501910 2_2:-52495938 None:intergenic
AGAAGTGGGAATGGGAGTGT+TGG 0.505103 2_2:-52495370 None:intergenic
GTTCCATTGGTGGCAAATGA+TGG 0.508291 2_2:+52496262 Msa0256980:CDS
TGAAATTGCTGACCTACCTA+TGG 0.522453 2_2:+52495814 Msa0256980:CDS
CAGATTATGAAAGCTGATCC+TGG 0.522799 2_2:+52496352 Msa0256980:CDS
TAATGAGGTATGTGTGAAGA+AGG 0.525810 2_2:+52496000 Msa0256980:CDS
TTCTCGCAAAGAAGTGGGAA+TGG 0.528027 2_2:-52495379 None:intergenic
CCCATTCTTGCAATCAACAA+TGG 0.538082 2_2:-52494914 None:intergenic
TTTAGGAGTACTCTCCATCA+TGG 0.550450 2_2:+52496467 Msa0256980:exon
GTGAAGAAGGCAGCTGCAGA+AGG 0.555483 2_2:+52496013 Msa0256980:CDS
GAACTGAAATGAAAAGGAGA+AGG 0.556891 2_2:-52494946 None:intergenic
AAATCCAATTAGTGTTCCCA+AGG 0.558642 2_2:-52496059 None:intergenic
ATTTATGTTACACAATGCCA+TGG 0.562016 2_2:-52496499 None:intergenic
AGAAGTTCTCGCAAAGAAGT+GGG 0.563836 2_2:-52495384 None:intergenic
TCTCGCAAAGAAGTGGGAAT+GGG 0.565013 2_2:-52495378 None:intergenic
AACAGCAACATTGGCACCAT+AGG 0.567015 2_2:-52496446 None:intergenic
TGTGTGTCGTCGACTTCCAT+AGG 0.575986 2_2:-52495830 None:intergenic
GTACTCCTAAACAGCAACAT+TGG 0.581094 2_2:-52496455 None:intergenic
CTTCTAAAGCCTGGACCAGA+AGG 0.582982 2_2:+52495402 Msa0256980:CDS
AGAAGGATACACATTTAATG+AGG 0.587845 2_2:+52495985 Msa0256980:CDS
TGTCGTCGACTTCCATAGGT+AGG 0.613856 2_2:-52495826 None:intergenic
TGACAAACAGGTTTACTACC+AGG 0.616609 2_2:-52496370 None:intergenic
CATGGTGTGTCCAACCATGA+TGG 0.623792 2_2:-52496481 None:intergenic
AACACCATAAGGTTGTGCAA+AGG 0.645055 2_2:-52495927 None:intergenic
CATCATGGTTGGACACACCA+TGG 0.652600 2_2:+52496482 Msa0256980:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTAAGAAACAATTTAAAATA+TGG + chr2_2:52495072-52495091 Msa0256980:intron 10.0%
!! ATGTTATAATATAATGAAAA+TGG + chr2_2:52496641-52496660 Msa0256980:three_prime_UTR 10.0%
!!! TTAAGAAACAATTTAAAATA+TGG + chr2_2:52495072-52495091 Msa0256980:intron 10.0%
!! ATGTTATAATATAATGAAAA+TGG + chr2_2:52496641-52496660 Msa0256980:three_prime_UTR 10.0%
!! TATCTTAAATTTAAAGACAT+AGG + chr2_2:52495716-52495735 Msa0256980:intron 15.0%
!!! TAAATCTAATAGAAGTTTTA+TGG + chr2_2:52495760-52495779 Msa0256980:intron 15.0%
!!! ATTCATTTTTTTTTATCAAC+AGG + chr2_2:52495788-52495807 Msa0256980:intron 15.0%
!! TATCTTAAATTTAAAGACAT+AGG + chr2_2:52495716-52495735 Msa0256980:intron 15.0%
!!! TAAATCTAATAGAAGTTTTA+TGG + chr2_2:52495760-52495779 Msa0256980:intron 15.0%
!!! ATTCATTTTTTTTTATCAAC+AGG + chr2_2:52495788-52495807 Msa0256980:intron 15.0%
!!! GCAATTAAAGTATTATTTGT+GGG + chr2_2:52495094-52495113 Msa0256980:intron 20.0%
!!! CAATTAAAGTATTATTTGTG+GGG + chr2_2:52495095-52495114 Msa0256980:intron 20.0%
!!! AATTAAAGTATTATTTGTGG+GGG + chr2_2:52495096-52495115 Msa0256980:intron 20.0%
!!! ATTTTTTTTAAGTAGTTCAG+TGG + chr2_2:52495464-52495483 Msa0256980:intron 20.0%
!! ACAAATGATGTCTTAATATA+AGG - chr2_2:52495675-52495694 None:intergenic 20.0%
!!! TTGTTTGAAACTTTTAAGAT+AGG + chr2_2:52495691-52495710 Msa0256980:intron 20.0%
!!! TGTTCATAGAAAAAAGTTTT+TGG - chr2_2:52495860-52495879 None:intergenic 20.0%
!! ATTAAAGACATTGAAAAAGA+AGG + chr2_2:52495968-52495987 Msa0256980:CDS 20.0%
!!! TTTTATTGTCATGAAATTCA+TGG + chr2_2:52496223-52496242 Msa0256980:CDS 20.0%
!! GAAAAAAAAATTCTACAATG+AGG - chr2_2:52496673-52496692 None:intergenic 20.0%
!! ATTGAAATCAGAAAATTTAG+AGG + chr2_2:52496845-52496864 Msa0256980:three_prime_UTR 20.0%
!!! GCAATTAAAGTATTATTTGT+GGG + chr2_2:52495094-52495113 Msa0256980:intron 20.0%
!!! CAATTAAAGTATTATTTGTG+GGG + chr2_2:52495095-52495114 Msa0256980:intron 20.0%
!!! AATTAAAGTATTATTTGTGG+GGG + chr2_2:52495096-52495115 Msa0256980:intron 20.0%
!!! ATTTTTTTTAAGTAGTTCAG+TGG + chr2_2:52495464-52495483 Msa0256980:intron 20.0%
!! ACAAATGATGTCTTAATATA+AGG - chr2_2:52495675-52495694 None:intergenic 20.0%
!!! TTGTTTGAAACTTTTAAGAT+AGG + chr2_2:52495691-52495710 Msa0256980:intron 20.0%
!!! TGTTCATAGAAAAAAGTTTT+TGG - chr2_2:52495860-52495879 None:intergenic 20.0%
!! ATTAAAGACATTGAAAAAGA+AGG + chr2_2:52495968-52495987 Msa0256980:CDS 20.0%
!!! TTTTATTGTCATGAAATTCA+TGG + chr2_2:52496223-52496242 Msa0256980:CDS 20.0%
!! GAAAAAAAAATTCTACAATG+AGG - chr2_2:52496673-52496692 None:intergenic 20.0%
!! ATTGAAATCAGAAAATTTAG+AGG + chr2_2:52496845-52496864 Msa0256980:three_prime_UTR 20.0%
!! GGCAATTAAAGTATTATTTG+TGG + chr2_2:52495093-52495112 Msa0256980:intron 25.0%
!! AAAATTTTGTGCAAAATCCT+TGG + chr2_2:52496042-52496061 Msa0256980:CDS 25.0%
!! AAATTTTGTGCAAAATCCTT+GGG + chr2_2:52496043-52496062 Msa0256980:CDS 25.0%
!!! ATAAAATAGCCTTATTTCCA+AGG - chr2_2:52496399-52496418 None:intergenic 25.0%
!!! TTGGAAATAAGGCTATTTTA+TGG + chr2_2:52496398-52496417 Msa0256980:CDS 25.0%
!!! TGGAAATAAGGCTATTTTAT+GGG + chr2_2:52496399-52496418 Msa0256980:CDS 25.0%
!! ATTTTATGGGTACCTAATTT+AGG + chr2_2:52496412-52496431 Msa0256980:CDS 25.0%
!! TTTCGTGAGTAAATGTTTTT+AGG + chr2_2:52496689-52496708 Msa0256980:three_prime_UTR 25.0%
!!! TTCGTGAGTAAATGTTTTTA+GGG + chr2_2:52496690-52496709 Msa0256980:three_prime_UTR 25.0%
!!! TTTTTAGGGCTAAAAATGTT+TGG + chr2_2:52496704-52496723 Msa0256980:three_prime_UTR 25.0%
!!! TTTTAGGGCTAAAAATGTTT+GGG + chr2_2:52496705-52496724 Msa0256980:three_prime_UTR 25.0%
! TTGTAACGAGAAAAAAAAGT+AGG + chr2_2:52496778-52496797 Msa0256980:three_prime_UTR 25.0%
!! GGCAATTAAAGTATTATTTG+TGG + chr2_2:52495093-52495112 Msa0256980:intron 25.0%
!! AAAATTTTGTGCAAAATCCT+TGG + chr2_2:52496042-52496061 Msa0256980:CDS 25.0%
!! AAATTTTGTGCAAAATCCTT+GGG + chr2_2:52496043-52496062 Msa0256980:CDS 25.0%
!!! ATAAAATAGCCTTATTTCCA+AGG - chr2_2:52496399-52496418 None:intergenic 25.0%
!!! TTGGAAATAAGGCTATTTTA+TGG + chr2_2:52496398-52496417 Msa0256980:CDS 25.0%
!!! TGGAAATAAGGCTATTTTAT+GGG + chr2_2:52496399-52496418 Msa0256980:CDS 25.0%
!! ATTTTATGGGTACCTAATTT+AGG + chr2_2:52496412-52496431 Msa0256980:CDS 25.0%
!! TTTCGTGAGTAAATGTTTTT+AGG + chr2_2:52496689-52496708 Msa0256980:three_prime_UTR 25.0%
!!! TTCGTGAGTAAATGTTTTTA+GGG + chr2_2:52496690-52496709 Msa0256980:three_prime_UTR 25.0%
!!! TTTTTAGGGCTAAAAATGTT+TGG + chr2_2:52496704-52496723 Msa0256980:three_prime_UTR 25.0%
!!! TTTTAGGGCTAAAAATGTTT+GGG + chr2_2:52496705-52496724 Msa0256980:three_prime_UTR 25.0%
! TTGTAACGAGAAAAAAAAGT+AGG + chr2_2:52496778-52496797 Msa0256980:three_prime_UTR 25.0%
!! AGAATTCTTAAGTGTTGAGA+GGG - chr2_2:52495017-52495036 None:intergenic 30.0%
!! AAGAATTCTTAAGTGTTGAG+AGG - chr2_2:52495018-52495037 None:intergenic 30.0%
TCTCAACACTTAAGAATTCT+TGG + chr2_2:52495017-52495036 Msa0256980:intron 30.0%
AGTTTATATAGTGCATCAGT+TGG - chr2_2:52495053-52495072 None:intergenic 30.0%
AACCTTATGGTGTTTATACA+TGG + chr2_2:52495936-52495955 Msa0256980:CDS 30.0%
ATCCATGTATAAACACCATA+AGG - chr2_2:52495941-52495960 None:intergenic 30.0%
AGAAGGATACACATTTAATG+AGG + chr2_2:52495985-52496004 Msa0256980:CDS 30.0%
GGATTTGCAATTTCTAAACT+TGG + chr2_2:52496076-52496095 Msa0256980:CDS 30.0%
TTGTTCATGCTTATCAATGA+AGG - chr2_2:52496128-52496147 None:intergenic 30.0%
! TCATAGGTTGAACTTTCAAA+AGG + chr2_2:52496195-52496214 Msa0256980:CDS 30.0%
! AAATGATGGAACAAAAACTC+AGG + chr2_2:52496276-52496295 Msa0256980:CDS 30.0%
! TTGTCATTTCCTTGGAAATA+AGG + chr2_2:52496387-52496406 Msa0256980:CDS 30.0%
ATTTATGTTACACAATGCCA+TGG - chr2_2:52496502-52496521 None:intergenic 30.0%
! TACTTTTATGTACCCTAAAG+AGG + chr2_2:52496588-52496607 Msa0256980:three_prime_UTR 30.0%
!! AGAATTCTTAAGTGTTGAGA+GGG - chr2_2:52495017-52495036 None:intergenic 30.0%
!! AAGAATTCTTAAGTGTTGAG+AGG - chr2_2:52495018-52495037 None:intergenic 30.0%
TCTCAACACTTAAGAATTCT+TGG + chr2_2:52495017-52495036 Msa0256980:intron 30.0%
AGTTTATATAGTGCATCAGT+TGG - chr2_2:52495053-52495072 None:intergenic 30.0%
AACCTTATGGTGTTTATACA+TGG + chr2_2:52495936-52495955 Msa0256980:CDS 30.0%
ATCCATGTATAAACACCATA+AGG - chr2_2:52495941-52495960 None:intergenic 30.0%
AGAAGGATACACATTTAATG+AGG + chr2_2:52495985-52496004 Msa0256980:CDS 30.0%
GGATTTGCAATTTCTAAACT+TGG + chr2_2:52496076-52496095 Msa0256980:CDS 30.0%
TTGTTCATGCTTATCAATGA+AGG - chr2_2:52496128-52496147 None:intergenic 30.0%
! TCATAGGTTGAACTTTCAAA+AGG + chr2_2:52496195-52496214 Msa0256980:CDS 30.0%
! AAATGATGGAACAAAAACTC+AGG + chr2_2:52496276-52496295 Msa0256980:CDS 30.0%
! TTGTCATTTCCTTGGAAATA+AGG + chr2_2:52496387-52496406 Msa0256980:CDS 30.0%
ATTTATGTTACACAATGCCA+TGG - chr2_2:52496502-52496521 None:intergenic 30.0%
! TACTTTTATGTACCCTAAAG+AGG + chr2_2:52496588-52496607 Msa0256980:three_prime_UTR 30.0%
! TCCATTGTTGATTGCAAGAA+TGG + chr2_2:52494913-52494932 Msa0256980:exon 35.0%
! CCATTGTTGATTGCAAGAAT+GGG + chr2_2:52494914-52494933 Msa0256980:exon 35.0%
GAACTGAAATGAAAAGGAGA+AGG - chr2_2:52494949-52494968 None:intergenic 35.0%
CAGAGAGAACTGAAATGAAA+AGG - chr2_2:52494955-52494974 None:intergenic 35.0%
! AAAGTATTATTTGTGGGGGT+TGG + chr2_2:52495100-52495119 Msa0256980:intron 35.0%
ACTGCCTGAAGAAGAATATT+GGG + chr2_2:52495335-52495354 Msa0256980:CDS 35.0%
AAGAATATTGGGAAGCTGTT+TGG + chr2_2:52495346-52495365 Msa0256980:CDS 35.0%
ATGTAATGCAAACAACCTTC+TGG - chr2_2:52495420-52495439 None:intergenic 35.0%
CTTTATGAACATAACTCAGC+TGG - chr2_2:52495563-52495582 None:intergenic 35.0%
! TATGAACATGAGCTTTATCC+AGG + chr2_2:52495872-52495891 Msa0256980:CDS 35.0%
!! TGCACTTTCATTGTTTTTCC+TGG - chr2_2:52495893-52495912 None:intergenic 35.0%
TAATGAGGTATGTGTGAAGA+AGG + chr2_2:52496000-52496019 Msa0256980:CDS 35.0%
AAATCCTTGGGAACACTAAT+TGG + chr2_2:52496055-52496074 Msa0256980:CDS 35.0%
AAATCCAATTAGTGTTCCCA+AGG - chr2_2:52496062-52496081 None:intergenic 35.0%
ATAGAGTCAGTGCAAAATCT+TGG + chr2_2:52496154-52496173 Msa0256980:CDS 35.0%
! TCATGGAACAACAGCTTTTA+TGG + chr2_2:52496240-52496259 Msa0256980:CDS 35.0%
! AACAGCTTTTATGGTTCCAT+TGG + chr2_2:52496249-52496268 Msa0256980:CDS 35.0%
! AAACCTGTTTGTCATTTCCT+TGG + chr2_2:52496379-52496398 Msa0256980:CDS 35.0%
TTTCCAAGGAAATGACAAAC+AGG - chr2_2:52496385-52496404 None:intergenic 35.0%
! ATGGGTACCTAATTTAGGTT+TGG + chr2_2:52496417-52496436 Msa0256980:CDS 35.0%
GCATTGTCCAAACCTAAATT+AGG - chr2_2:52496427-52496446 None:intergenic 35.0%
CTAGCTAATTAAGCTAGAGT+TGG + chr2_2:52496557-52496576 Msa0256980:three_prime_UTR 35.0%
! TCCATTGTTGATTGCAAGAA+TGG + chr2_2:52494913-52494932 Msa0256980:exon 35.0%
! CCATTGTTGATTGCAAGAAT+GGG + chr2_2:52494914-52494933 Msa0256980:exon 35.0%
GAACTGAAATGAAAAGGAGA+AGG - chr2_2:52494949-52494968 None:intergenic 35.0%
CAGAGAGAACTGAAATGAAA+AGG - chr2_2:52494955-52494974 None:intergenic 35.0%
! AAAGTATTATTTGTGGGGGT+TGG + chr2_2:52495100-52495119 Msa0256980:intron 35.0%
ACTGCCTGAAGAAGAATATT+GGG + chr2_2:52495335-52495354 Msa0256980:CDS 35.0%
AAGAATATTGGGAAGCTGTT+TGG + chr2_2:52495346-52495365 Msa0256980:CDS 35.0%
ATGTAATGCAAACAACCTTC+TGG - chr2_2:52495420-52495439 None:intergenic 35.0%
CTTTATGAACATAACTCAGC+TGG - chr2_2:52495563-52495582 None:intergenic 35.0%
! TATGAACATGAGCTTTATCC+AGG + chr2_2:52495872-52495891 Msa0256980:CDS 35.0%
!! TGCACTTTCATTGTTTTTCC+TGG - chr2_2:52495893-52495912 None:intergenic 35.0%
TAATGAGGTATGTGTGAAGA+AGG + chr2_2:52496000-52496019 Msa0256980:CDS 35.0%
AAATCCTTGGGAACACTAAT+TGG + chr2_2:52496055-52496074 Msa0256980:CDS 35.0%
AAATCCAATTAGTGTTCCCA+AGG - chr2_2:52496062-52496081 None:intergenic 35.0%
ATAGAGTCAGTGCAAAATCT+TGG + chr2_2:52496154-52496173 Msa0256980:CDS 35.0%
! TCATGGAACAACAGCTTTTA+TGG + chr2_2:52496240-52496259 Msa0256980:CDS 35.0%
! AACAGCTTTTATGGTTCCAT+TGG + chr2_2:52496249-52496268 Msa0256980:CDS 35.0%
! AAACCTGTTTGTCATTTCCT+TGG + chr2_2:52496379-52496398 Msa0256980:CDS 35.0%
TTTCCAAGGAAATGACAAAC+AGG - chr2_2:52496385-52496404 None:intergenic 35.0%
! ATGGGTACCTAATTTAGGTT+TGG + chr2_2:52496417-52496436 Msa0256980:CDS 35.0%
GCATTGTCCAAACCTAAATT+AGG - chr2_2:52496427-52496446 None:intergenic 35.0%
CTAGCTAATTAAGCTAGAGT+TGG + chr2_2:52496557-52496576 Msa0256980:three_prime_UTR 35.0%
CCCATTCTTGCAATCAACAA+TGG - chr2_2:52494917-52494936 None:intergenic 40.0%
CAATATTCTTCTTCAGGCAG+TGG - chr2_2:52495336-52495355 None:intergenic 40.0%
CACTGCCTGAAGAAGAATAT+TGG + chr2_2:52495334-52495353 Msa0256980:CDS 40.0%
GCTTCCCAATATTCTTCTTC+AGG - chr2_2:52495342-52495361 None:intergenic 40.0%
AGAAGTTCTCGCAAAGAAGT+GGG - chr2_2:52495387-52495406 None:intergenic 40.0%
TAGAAGTTCTCGCAAAGAAG+TGG - chr2_2:52495388-52495407 None:intergenic 40.0%
TGAACATAACTCAGCTGGTA+GGG - chr2_2:52495558-52495577 None:intergenic 40.0%
ATGAACATAACTCAGCTGGT+AGG - chr2_2:52495559-52495578 None:intergenic 40.0%
TGAAATTGCTGACCTACCTA+TGG + chr2_2:52495814-52495833 Msa0256980:CDS 40.0%
ACACCATAAGGTTGTGCAAA+GGG - chr2_2:52495929-52495948 None:intergenic 40.0%
AACACCATAAGGTTGTGCAA+AGG - chr2_2:52495930-52495949 None:intergenic 40.0%
AGAAAGCAGTGATGTGTCAT+AGG + chr2_2:52496179-52496198 Msa0256980:CDS 40.0%
! AGCTTTTATGGTTCCATTGG+TGG + chr2_2:52496252-52496271 Msa0256980:CDS 40.0%
GTTTGTCATGCTGATACTTC+AGG + chr2_2:52496307-52496326 Msa0256980:CDS 40.0%
CAGATTATGAAAGCTGATCC+TGG + chr2_2:52496352-52496371 Msa0256980:CDS 40.0%
TGACAAACAGGTTTACTACC+AGG - chr2_2:52496373-52496392 None:intergenic 40.0%
TTAGGTTTGGACAATGCCTA+TGG + chr2_2:52496430-52496449 Msa0256980:CDS 40.0%
GTACTCCTAAACAGCAACAT+TGG - chr2_2:52496458-52496477 None:intergenic 40.0%
!! TTTAGGAGTACTCTCCATCA+TGG + chr2_2:52496467-52496486 Msa0256980:exon 40.0%
GCAAAGATTGTGCCTCTTTA+GGG - chr2_2:52496603-52496622 None:intergenic 40.0%
AGCAAAGATTGTGCCTCTTT+AGG - chr2_2:52496604-52496623 None:intergenic 40.0%
CCCATTCTTGCAATCAACAA+TGG - chr2_2:52494917-52494936 None:intergenic 40.0%
CAATATTCTTCTTCAGGCAG+TGG - chr2_2:52495336-52495355 None:intergenic 40.0%
CACTGCCTGAAGAAGAATAT+TGG + chr2_2:52495334-52495353 Msa0256980:CDS 40.0%
GCTTCCCAATATTCTTCTTC+AGG - chr2_2:52495342-52495361 None:intergenic 40.0%
AGAAGTTCTCGCAAAGAAGT+GGG - chr2_2:52495387-52495406 None:intergenic 40.0%
TAGAAGTTCTCGCAAAGAAG+TGG - chr2_2:52495388-52495407 None:intergenic 40.0%
TGAACATAACTCAGCTGGTA+GGG - chr2_2:52495558-52495577 None:intergenic 40.0%
ATGAACATAACTCAGCTGGT+AGG - chr2_2:52495559-52495578 None:intergenic 40.0%
TGAAATTGCTGACCTACCTA+TGG + chr2_2:52495814-52495833 Msa0256980:CDS 40.0%
ACACCATAAGGTTGTGCAAA+GGG - chr2_2:52495929-52495948 None:intergenic 40.0%
AACACCATAAGGTTGTGCAA+AGG - chr2_2:52495930-52495949 None:intergenic 40.0%
AGAAAGCAGTGATGTGTCAT+AGG + chr2_2:52496179-52496198 Msa0256980:CDS 40.0%
! AGCTTTTATGGTTCCATTGG+TGG + chr2_2:52496252-52496271 Msa0256980:CDS 40.0%
GTTTGTCATGCTGATACTTC+AGG + chr2_2:52496307-52496326 Msa0256980:CDS 40.0%
CAGATTATGAAAGCTGATCC+TGG + chr2_2:52496352-52496371 Msa0256980:CDS 40.0%
TGACAAACAGGTTTACTACC+AGG - chr2_2:52496373-52496392 None:intergenic 40.0%
TTAGGTTTGGACAATGCCTA+TGG + chr2_2:52496430-52496449 Msa0256980:CDS 40.0%
GTACTCCTAAACAGCAACAT+TGG - chr2_2:52496458-52496477 None:intergenic 40.0%
!! TTTAGGAGTACTCTCCATCA+TGG + chr2_2:52496467-52496486 Msa0256980:exon 40.0%
GCAAAGATTGTGCCTCTTTA+GGG - chr2_2:52496603-52496622 None:intergenic 40.0%
AGCAAAGATTGTGCCTCTTT+AGG - chr2_2:52496604-52496623 None:intergenic 40.0%
!! CTTAAGTGTTGAGAGGGTCA+TGG - chr2_2:52495011-52495030 None:intergenic 45.0%
TCTCGCAAAGAAGTGGGAAT+GGG - chr2_2:52495381-52495400 None:intergenic 45.0%
TTCTCGCAAAGAAGTGGGAA+TGG - chr2_2:52495382-52495401 None:intergenic 45.0%
!! TTGCGAGAACTTCTAAAGCC+TGG + chr2_2:52495393-52495412 Msa0256980:CDS 45.0%
AGAAGTCTCGAGCTCAAACA+TGG + chr2_2:52495510-52495529 Msa0256980:intron 45.0%
! GTTCCATTGGTGGCAAATGA+TGG + chr2_2:52496262-52496281 Msa0256980:CDS 45.0%
GTTCCATCATTTGCCACCAA+TGG - chr2_2:52496268-52496287 None:intergenic 45.0%
AACAGCAACATTGGCACCAT+AGG - chr2_2:52496449-52496468 None:intergenic 45.0%
!! TGGTGCCAATGTTGCTGTTT+AGG + chr2_2:52496450-52496469 Msa0256980:CDS 45.0%
!! CTTAAGTGTTGAGAGGGTCA+TGG - chr2_2:52495011-52495030 None:intergenic 45.0%
TCTCGCAAAGAAGTGGGAAT+GGG - chr2_2:52495381-52495400 None:intergenic 45.0%
TTCTCGCAAAGAAGTGGGAA+TGG - chr2_2:52495382-52495401 None:intergenic 45.0%
!! TTGCGAGAACTTCTAAAGCC+TGG + chr2_2:52495393-52495412 Msa0256980:CDS 45.0%
AGAAGTCTCGAGCTCAAACA+TGG + chr2_2:52495510-52495529 Msa0256980:intron 45.0%
! GTTCCATTGGTGGCAAATGA+TGG + chr2_2:52496262-52496281 Msa0256980:CDS 45.0%
GTTCCATCATTTGCCACCAA+TGG - chr2_2:52496268-52496287 None:intergenic 45.0%
AACAGCAACATTGGCACCAT+AGG - chr2_2:52496449-52496468 None:intergenic 45.0%
!! TGGTGCCAATGTTGCTGTTT+AGG + chr2_2:52496450-52496469 Msa0256980:CDS 45.0%
GAGAGGGTCATGGAGATGAA+AGG - chr2_2:52495001-52495020 None:intergenic 50.0%
!! GAAGTGGGAATGGGAGTGTT+GGG - chr2_2:52495372-52495391 None:intergenic 50.0%
! AGAAGTGGGAATGGGAGTGT+TGG - chr2_2:52495373-52495392 None:intergenic 50.0%
!! CTTCTAAAGCCTGGACCAGA+AGG + chr2_2:52495402-52495421 Msa0256980:CDS 50.0%
TGCAAACAACCTTCTGGTCC+AGG - chr2_2:52495414-52495433 None:intergenic 50.0%
!! TGTCGTCGACTTCCATAGGT+AGG - chr2_2:52495829-52495848 None:intergenic 50.0%
!! TGTGTGTCGTCGACTTCCAT+AGG - chr2_2:52495833-52495852 None:intergenic 50.0%
CGTCCCTTTGCACAACCTTA+TGG + chr2_2:52495923-52495942 Msa0256980:CDS 50.0%
! GGAGTACTCTCCATCATGGT+TGG + chr2_2:52496471-52496490 Msa0256980:exon 50.0%
CATGGTGTGTCCAACCATGA+TGG - chr2_2:52496484-52496503 None:intergenic 50.0%
CATCATGGTTGGACACACCA+TGG + chr2_2:52496482-52496501 Msa0256980:three_prime_UTR 50.0%
GAGAGGGTCATGGAGATGAA+AGG - chr2_2:52495001-52495020 None:intergenic 50.0%
!! GAAGTGGGAATGGGAGTGTT+GGG - chr2_2:52495372-52495391 None:intergenic 50.0%
! AGAAGTGGGAATGGGAGTGT+TGG - chr2_2:52495373-52495392 None:intergenic 50.0%
!! CTTCTAAAGCCTGGACCAGA+AGG + chr2_2:52495402-52495421 Msa0256980:CDS 50.0%
TGCAAACAACCTTCTGGTCC+AGG - chr2_2:52495414-52495433 None:intergenic 50.0%
!! TGTCGTCGACTTCCATAGGT+AGG - chr2_2:52495829-52495848 None:intergenic 50.0%
!! TGTGTGTCGTCGACTTCCAT+AGG - chr2_2:52495833-52495852 None:intergenic 50.0%
CGTCCCTTTGCACAACCTTA+TGG + chr2_2:52495923-52495942 Msa0256980:CDS 50.0%
! GGAGTACTCTCCATCATGGT+TGG + chr2_2:52496471-52496490 Msa0256980:exon 50.0%
CATGGTGTGTCCAACCATGA+TGG - chr2_2:52496484-52496503 None:intergenic 50.0%
CATCATGGTTGGACACACCA+TGG + chr2_2:52496482-52496501 Msa0256980:three_prime_UTR 50.0%
GATGTGCAGCTTGCACTTGC+AGG + chr2_2:52495297-52495316 Msa0256980:intron 55.0%
CTTCTTCAGGCAGTGGTGCA+TGG - chr2_2:52495329-52495348 None:intergenic 55.0%
GTGAAGAAGGCAGCTGCAGA+AGG + chr2_2:52496013-52496032 Msa0256980:CDS 55.0%
GATGTGCAGCTTGCACTTGC+AGG + chr2_2:52495297-52495316 Msa0256980:intron 55.0%
CTTCTTCAGGCAGTGGTGCA+TGG - chr2_2:52495329-52495348 None:intergenic 55.0%
GTGAAGAAGGCAGCTGCAGA+AGG + chr2_2:52496013-52496032 Msa0256980:CDS 55.0%
Chromosome Type Strat End Strand Name
chr2_2 gene 52494878 52496872 52494878 ID=Msa0256980;Name=Msa0256980
chr2_2 mRNA 52494878 52496872 52494878 ID=Msa0256980-mRNA-1;Parent=Msa0256980;Name=Msa0256980-mRNA-1;_AED=0.00;_eAED=0.00;_QI=54|1|1|1|1|1|3|401|273
chr2_2 exon 52494878 52494973 52494878 ID=Msa0256980-mRNA-1:exon:15783;Parent=Msa0256980-mRNA-1
chr2_2 exon 52495306 52495423 52495306 ID=Msa0256980-mRNA-1:exon:15784;Parent=Msa0256980-mRNA-1
chr2_2 exon 52495810 52496872 52495810 ID=Msa0256980-mRNA-1:exon:15785;Parent=Msa0256980-mRNA-1
chr2_2 five_prime_UTR 52494878 52494931 52494878 ID=Msa0256980-mRNA-1:five_prime_utr;Parent=Msa0256980-mRNA-1
chr2_2 CDS 52494932 52494973 52494932 ID=Msa0256980-mRNA-1:cds;Parent=Msa0256980-mRNA-1
chr2_2 CDS 52495306 52495423 52495306 ID=Msa0256980-mRNA-1:cds;Parent=Msa0256980-mRNA-1
chr2_2 CDS 52495810 52496471 52495810 ID=Msa0256980-mRNA-1:cds;Parent=Msa0256980-mRNA-1
chr2_2 three_prime_UTR 52496472 52496872 52496472 ID=Msa0256980-mRNA-1:three_prime_utr;Parent=Msa0256980-mRNA-1
Gene Sequence

>Msa0256980

ATGGGATTTCATCACCTTCTCCTTTTCATTTCAGTTCTCTCTCTTGCACTTGCAGGAGAAAGCCATGCACCACTGCCTGAAGAAGAATATTGGGAAGCTGTTTGGCCCAACACTCCCATTCCCACTTCTTTGCGAGAACTTCTAAAGCCTGGACCAGAAGGTGTTGAAATTGCTGACCTACCTATGGAAGTCGACGACACACAATATCCAAAAACTTTTTTCTATGAACATGAGCTTTATCCAGGAAAAACAATGAAAGTGCAATTCAGCAAGCGTCCCTTTGCACAACCTTATGGTGTTTATACATGGATGCGTGAAATTAAAGACATTGAAAAAGAAGGATACACATTTAATGAGGTATGTGTGAAGAAGGCAGCTGCAGAAGGAGAACAAAAATTTTGTGCAAAATCCTTGGGAACACTAATTGGATTTGCAATTTCTAAACTTGGAAAAAATATTCAAGCACTTTCAAGTTCCTTCATTGATAAGCATGAACAATACAAAATAGAGTCAGTGCAAAATCTTGGAGAGAAAGCAGTGATGTGTCATAGGTTGAACTTTCAAAAGGTTGTATTTTATTGTCATGAAATTCATGGAACAACAGCTTTTATGGTTCCATTGGTGGCAAATGATGGAACAAAAACTCAGGCACTCGCTGTTTGTCATGCTGATACTTCAGGCATGAATCATGAAATGCTGCAACAGATTATGAAAGCTGATCCTGGTAGTAAACCTGTTTGTCATTTCCTTGGAAATAAGGCTATTTTATGGGTACCTAATTTAGGTTTGGACAATGCCTATGGTGCCAATGTTGCTGTTTAG
Protein sequence

>Msa0256980

MGFHHLLLFISVLSLALAGESHAPLPEEEYWEAVWPNTPIPTSLRELLKPGPEGVEIADLPMEVDDTQYPKTFFYEHELYPGKTMKVQFSKRPFAQPYGVYTWMREIKDIEKEGYTFNEVCVKKAAAEGEQKFCAKSLGTLIGFAISKLGKNIQALSSSFIDKHEQYKIESVQNLGEKAVMCHRLNFQKVVFYCHEIHGTTAFMVPLVANDGTKTQALAVCHADTSGMNHEMLQQIMKADPGSKPVCHFLGNKAILWVPNLGLDNAYGANVAV*