AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0411870

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0411870 MtrunA17_Chr3g0103341 97.849 186 3 1 1 185 1 186 1.60e-132 373
Msa0411870 MtrunA17_Chr8g0358741 41.259 143 80 1 41 183 68 206 1.16e-25 101
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0411870 AT5G56120.1 54.787 188 78 3 1 182 1 187 2.42e-58 186
Msa0411870 AT3G12870.1 38.667 150 92 0 34 183 11 160 1.21e-29 110

Find 64 sgRNAs with CRISPR-Local

Find 178 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCATGTAATTTCTCTTATC+AGG 0.256680 3_2:+54472431 Msa0411870:three_prime_UTR
ACATGATATGCTTGTTGATT+AGG 0.271541 3_2:-54472220 None:intergenic
TGATGATGTTCTTGGTCTAC+TGG 0.277729 3_2:-54471229 None:intergenic
TTCCACCACCACCATTCATT+TGG 0.309071 3_2:-54471516 None:intergenic
AGGTGTTGGGAGGTGTATTC+AGG 0.310456 3_2:+54471672 Msa0411870:CDS
GATTCAGAGATTTCGGTTAA+AGG 0.322599 3_2:-54471313 None:intergenic
CCCGGGTGATTCAGAGATTT+CGG 0.323102 3_2:-54471320 None:intergenic
ACCTCTTACTCTTAAAGCTT+TGG 0.355477 3_2:+54471344 Msa0411870:CDS
TAGAATTGAGTGCACATATC+AGG 0.366380 3_2:+54472350 Msa0411870:three_prime_UTR
AAGTGAAATTGGTTAGGTAT+TGG 0.376344 3_2:+54471608 Msa0411870:CDS
GCCAAAGCTTTAAGAGTAAG+AGG 0.390218 3_2:-54471345 None:intergenic
TCCACCACCACCATTCATTT+GGG 0.390629 3_2:-54471515 None:intergenic
CCAACATTGCTTCATTCCTT+TGG 0.395413 3_2:-54472029 None:intergenic
ATGAAGTGAAAAGGAGTGTT+AGG 0.395626 3_2:-54471483 None:intergenic
AGGAATGATAGTAGAGGTGT+TGG 0.398719 3_2:+54471658 Msa0411870:CDS
ATTCAGAGATTTCGGTTAAA+GGG 0.409780 3_2:-54471312 None:intergenic
TGATAGATTCAATCCTTTGA+CGG 0.438010 3_2:+54472404 Msa0411870:three_prime_UTR
TTCAGGAATTGAGGATGAAA+GGG 0.438603 3_2:+54471689 Msa0411870:CDS
TCCGATTGATTTACTGCACT+TGG 0.440482 3_2:+54472052 Msa0411870:CDS
TGGTTCAAGTGAAATTGGTT+AGG 0.445316 3_2:+54471602 Msa0411870:CDS
TTCAAGAAAAGAAACAAGAA+TGG 0.448093 3_2:+54471399 Msa0411870:CDS
TTGATTTACTGCACTTGGCT+CGG 0.451535 3_2:+54472057 Msa0411870:CDS
GCTTCCAAGTTTGTTCTATG+TGG 0.453603 3_2:+54471859 Msa0411870:CDS
ACGATATGAGTCATTTGATC+TGG 0.454704 3_2:+54472116 Msa0411870:CDS
CAAACCACATAGAACAAACT+TGG 0.460260 3_2:-54471863 None:intergenic
GATCAATGGACTTCGGATCA+TGG 0.465206 3_2:+54472178 Msa0411870:three_prime_UTR
GTAGATTTAAGAGAGTTAAG+TGG 0.470216 3_2:+54472302 Msa0411870:three_prime_UTR
AAAATTCAATTTGTGAGAGA+TGG 0.487999 3_2:+54472378 Msa0411870:three_prime_UTR
GAATTGTTGATTTAGATCAA+TGG 0.488508 3_2:+54472164 Msa0411870:exon
GCCAAGTGCAGTAAATCAAT+CGG 0.494276 3_2:-54472053 None:intergenic
GATGATGTTCTTGGTCTACT+GGG 0.500369 3_2:-54471228 None:intergenic
GGAGGTGTATTCAGGAATTG+AGG 0.501941 3_2:+54471680 Msa0411870:CDS
CCAAAGGAATGAAGCAATGT+TGG 0.512491 3_2:+54472029 Msa0411870:CDS
TGGTTAGGTATTGGAAAGTG+TGG 0.515468 3_2:+54471617 Msa0411870:CDS
CTCTCTTAAATCTACTCACA+AGG 0.519552 3_2:-54472294 None:intergenic
CTTTATATAGCCAATACAAA+AGG 0.521425 3_2:-54472271 None:intergenic
CTAGCTAGTAATTGCGAAGA+CGG 0.523917 3_2:+54472141 Msa0411870:CDS
GTGGTGGAAGTTGTAAGATA+TGG 0.530191 3_2:+54471530 Msa0411870:CDS
GTGGAAGTTGTAAGATATGG+TGG 0.530843 3_2:+54471533 Msa0411870:CDS
CTTTAAGAGTAAGAGGTGCC+CGG 0.533485 3_2:-54471338 None:intergenic
GGAATGATAGTAGAGGTGTT+GGG 0.538678 3_2:+54471659 Msa0411870:CDS
ATTCAGGAATTGAGGATGAA+AGG 0.541806 3_2:+54471688 Msa0411870:CDS
AGGGAGAGGAATGATAGTAG+AGG 0.549423 3_2:+54471652 Msa0411870:CDS
ACCCAAATGAATGGTGGTGG+TGG 0.550058 3_2:+54471514 Msa0411870:CDS
TCCTGATAAGAGAAATTACA+TGG 0.555248 3_2:-54472432 None:intergenic
GCTTCATTCCTTTGGCAGAG+AGG 0.555837 3_2:-54472021 None:intergenic
CATAGAACAAACTTGGAAGC+AGG 0.560691 3_2:-54471856 None:intergenic
TGATTTAGATCAATGGACTT+CGG 0.562848 3_2:+54472171 Msa0411870:exon
TAGCTAGTAATTGCGAAGAC+GGG 0.570111 3_2:+54472142 Msa0411870:CDS
GGTTAGGTATTGGAAAGTGT+GGG 0.574203 3_2:+54471618 Msa0411870:CDS
GTTGAAACTGAAATCAATGA+TGG 0.577321 3_2:-54471559 None:intergenic
TTTAAGAGTAAGAGGTGCCC+GGG 0.579280 3_2:-54471337 None:intergenic
ACCGAAATCTCTGAATCACC+CGG 0.582927 3_2:+54471319 Msa0411870:CDS
TTACATGGATCATCCGTCAA+AGG 0.594398 3_2:-54472417 None:intergenic
CAGAGATTTCGGTTAAAGGG+TGG 0.602801 3_2:-54471309 None:intergenic
GGGAACAGTTACAAAGGGAG+AGG 0.615352 3_2:+54471638 Msa0411870:CDS
GACTCATATCGTGATTTCAA+CGG 0.621041 3_2:-54472105 None:intergenic
AAAACCCAAATGAATGGTGG+TGG 0.629504 3_2:+54471511 Msa0411870:CDS
AAGTGTGGGAACAGTTACAA+AGG 0.631462 3_2:+54471632 Msa0411870:CDS
ATGATAGTAGAGGTGTTGGG+AGG 0.635080 3_2:+54471662 Msa0411870:CDS
AGTGTGGGAACAGTTACAAA+GGG 0.635769 3_2:+54471633 Msa0411870:CDS
TTGAGTGCACATATCAGGAG+AGG 0.651983 3_2:+54472355 Msa0411870:three_prime_UTR
TCAGGAATTGAGGATGAAAG+GGG 0.667293 3_2:+54471690 Msa0411870:CDS
CCGAAATCTCTGAATCACCC+GGG 0.680661 3_2:+54471320 Msa0411870:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTGTTTATTGTTAATGATG+AGG + chr3_2:54471912-54471931 Msa0411870:intron 20.0%
!!! CTTTCTTAATTTGTGTTATT+TGG + chr3_2:54471950-54471969 Msa0411870:intron 20.0%
!!! GAGATTTGTTTAGTTTTTTA+TGG + chr3_2:54471979-54471998 Msa0411870:intron 20.0%
!! TTTGTTTATTGTTAATGATG+AGG + chr3_2:54471912-54471931 Msa0411870:intron 20.0%
!!! CTTTCTTAATTTGTGTTATT+TGG + chr3_2:54471950-54471969 Msa0411870:intron 20.0%
!!! GAGATTTGTTTAGTTTTTTA+TGG + chr3_2:54471979-54471998 Msa0411870:intron 20.0%
! TTCAAGAAAAGAAACAAGAA+TGG + chr3_2:54471399-54471418 Msa0411870:CDS 25.0%
!! ATCAAGATTATGATGATGTT+TGG + chr3_2:54471888-54471907 Msa0411870:intron 25.0%
!!! GAATTGTTGATTTAGATCAA+TGG + chr3_2:54472164-54472183 Msa0411870:exon 25.0%
! CTTTATATAGCCAATACAAA+AGG - chr3_2:54472274-54472293 None:intergenic 25.0%
! AAAATTCAATTTGTGAGAGA+TGG + chr3_2:54472378-54472397 Msa0411870:three_prime_UTR 25.0%
! TTCAAGAAAAGAAACAAGAA+TGG + chr3_2:54471399-54471418 Msa0411870:CDS 25.0%
!! ATCAAGATTATGATGATGTT+TGG + chr3_2:54471888-54471907 Msa0411870:intron 25.0%
!!! GAATTGTTGATTTAGATCAA+TGG + chr3_2:54472164-54472183 Msa0411870:exon 25.0%
! CTTTATATAGCCAATACAAA+AGG - chr3_2:54472274-54472293 None:intergenic 25.0%
! AAAATTCAATTTGTGAGAGA+TGG + chr3_2:54472378-54472397 Msa0411870:three_prime_UTR 25.0%
!!! TGTTGTTTTGATGATGTTCT+TGG - chr3_2:54471240-54471259 None:intergenic 30.0%
ATTCAGAGATTTCGGTTAAA+GGG - chr3_2:54471315-54471334 None:intergenic 30.0%
! TTCTTTTCTTGAAATGCGTT+TGG - chr3_2:54471392-54471411 None:intergenic 30.0%
! TGAAGTGAGTTGAAAAAGTT+CGG - chr3_2:54471437-54471456 None:intergenic 30.0%
! TCATTCTTCTTCATTTTCCA+TGG + chr3_2:54471454-54471473 Msa0411870:CDS 30.0%
!!! TTTTTTGCCATGAAGTGAAA+AGG - chr3_2:54471495-54471514 None:intergenic 30.0%
GTTGAAACTGAAATCAATGA+TGG - chr3_2:54471562-54471581 None:intergenic 30.0%
! TTCACTTGTGTTTGTGTTTT+TGG + chr3_2:54471582-54471601 Msa0411870:CDS 30.0%
!!! GTTTTTGGTTCAAGTGAAAT+TGG + chr3_2:54471597-54471616 Msa0411870:CDS 30.0%
AAGTGAAATTGGTTAGGTAT+TGG + chr3_2:54471608-54471627 Msa0411870:CDS 30.0%
!!! GTTTGTCTTGTTTGTTTTAC+AGG + chr3_2:54471823-54471842 Msa0411870:intron 30.0%
!!! TCTTGTTTGTTTTACAGGTT+TGG + chr3_2:54471828-54471847 Msa0411870:intron 30.0%
! TGATTTAGATCAATGGACTT+CGG + chr3_2:54472171-54472190 Msa0411870:exon 30.0%
! GCTTGTTGATTAGGTAAAAA+TGG - chr3_2:54472214-54472233 None:intergenic 30.0%
! ACATGATATGCTTGTTGATT+AGG - chr3_2:54472223-54472242 None:intergenic 30.0%
GTAGATTTAAGAGAGTTAAG+TGG + chr3_2:54472302-54472321 Msa0411870:three_prime_UTR 30.0%
TGATAGATTCAATCCTTTGA+CGG + chr3_2:54472404-54472423 Msa0411870:three_prime_UTR 30.0%
TCCATGTAATTTCTCTTATC+AGG + chr3_2:54472431-54472450 Msa0411870:three_prime_UTR 30.0%
TCCTGATAAGAGAAATTACA+TGG - chr3_2:54472435-54472454 None:intergenic 30.0%
!!! TGTTGTTTTGATGATGTTCT+TGG - chr3_2:54471240-54471259 None:intergenic 30.0%
ATTCAGAGATTTCGGTTAAA+GGG - chr3_2:54471315-54471334 None:intergenic 30.0%
! TTCTTTTCTTGAAATGCGTT+TGG - chr3_2:54471392-54471411 None:intergenic 30.0%
! TGAAGTGAGTTGAAAAAGTT+CGG - chr3_2:54471437-54471456 None:intergenic 30.0%
! TCATTCTTCTTCATTTTCCA+TGG + chr3_2:54471454-54471473 Msa0411870:CDS 30.0%
!!! TTTTTTGCCATGAAGTGAAA+AGG - chr3_2:54471495-54471514 None:intergenic 30.0%
GTTGAAACTGAAATCAATGA+TGG - chr3_2:54471562-54471581 None:intergenic 30.0%
! TTCACTTGTGTTTGTGTTTT+TGG + chr3_2:54471582-54471601 Msa0411870:CDS 30.0%
!!! GTTTTTGGTTCAAGTGAAAT+TGG + chr3_2:54471597-54471616 Msa0411870:CDS 30.0%
AAGTGAAATTGGTTAGGTAT+TGG + chr3_2:54471608-54471627 Msa0411870:CDS 30.0%
!!! GTTTGTCTTGTTTGTTTTAC+AGG + chr3_2:54471823-54471842 Msa0411870:intron 30.0%
!!! TCTTGTTTGTTTTACAGGTT+TGG + chr3_2:54471828-54471847 Msa0411870:intron 30.0%
! TGATTTAGATCAATGGACTT+CGG + chr3_2:54472171-54472190 Msa0411870:exon 30.0%
! GCTTGTTGATTAGGTAAAAA+TGG - chr3_2:54472214-54472233 None:intergenic 30.0%
! ACATGATATGCTTGTTGATT+AGG - chr3_2:54472223-54472242 None:intergenic 30.0%
GTAGATTTAAGAGAGTTAAG+TGG + chr3_2:54472302-54472321 Msa0411870:three_prime_UTR 30.0%
TGATAGATTCAATCCTTTGA+CGG + chr3_2:54472404-54472423 Msa0411870:three_prime_UTR 30.0%
TCCATGTAATTTCTCTTATC+AGG + chr3_2:54472431-54472450 Msa0411870:three_prime_UTR 30.0%
TCCTGATAAGAGAAATTACA+TGG - chr3_2:54472435-54472454 None:intergenic 30.0%
GATTCAGAGATTTCGGTTAA+AGG - chr3_2:54471316-54471335 None:intergenic 35.0%
ACCTCTTACTCTTAAAGCTT+TGG + chr3_2:54471344-54471363 Msa0411870:CDS 35.0%
!! ATGAAGTGAAAAGGAGTGTT+AGG - chr3_2:54471486-54471505 None:intergenic 35.0%
! TAACACTCCTTTTCACTTCA+TGG + chr3_2:54471485-54471504 Msa0411870:CDS 35.0%
TGGCAAAAAACCCAAATGAA+TGG + chr3_2:54471505-54471524 Msa0411870:CDS 35.0%
CAAAAAACCCAAATGAATGG+TGG + chr3_2:54471508-54471527 Msa0411870:CDS 35.0%
TGGTTCAAGTGAAATTGGTT+AGG + chr3_2:54471602-54471621 Msa0411870:CDS 35.0%
ATTCAGGAATTGAGGATGAA+AGG + chr3_2:54471688-54471707 Msa0411870:CDS 35.0%
TTCAGGAATTGAGGATGAAA+GGG + chr3_2:54471689-54471708 Msa0411870:CDS 35.0%
TCTAAAGAGTTGCAAGTGAA+TGG + chr3_2:54471727-54471746 Msa0411870:intron 35.0%
CTAAAGAGTTGCAAGTGAAT+GGG + chr3_2:54471728-54471747 Msa0411870:intron 35.0%
!! GTTCGAGTGTTGAGATTAAA+TGG + chr3_2:54471764-54471783 Msa0411870:intron 35.0%
TGTTGAGATTAAATGGACAG+TGG + chr3_2:54471771-54471790 Msa0411870:intron 35.0%
TTAAATGGACAGTGGTTACT+TGG + chr3_2:54471779-54471798 Msa0411870:intron 35.0%
CAAACCACATAGAACAAACT+TGG - chr3_2:54471866-54471885 None:intergenic 35.0%
GACTCATATCGTGATTTCAA+CGG - chr3_2:54472108-54472127 None:intergenic 35.0%
ACGATATGAGTCATTTGATC+TGG + chr3_2:54472116-54472135 Msa0411870:CDS 35.0%
!!! CTAACTTGCTCCTTTTGTAT+TGG + chr3_2:54472261-54472280 Msa0411870:three_prime_UTR 35.0%
CTCTCTTAAATCTACTCACA+AGG - chr3_2:54472297-54472316 None:intergenic 35.0%
TAGAATTGAGTGCACATATC+AGG + chr3_2:54472350-54472369 Msa0411870:three_prime_UTR 35.0%
GATTCAGAGATTTCGGTTAA+AGG - chr3_2:54471316-54471335 None:intergenic 35.0%
ACCTCTTACTCTTAAAGCTT+TGG + chr3_2:54471344-54471363 Msa0411870:CDS 35.0%
!! ATGAAGTGAAAAGGAGTGTT+AGG - chr3_2:54471486-54471505 None:intergenic 35.0%
! TAACACTCCTTTTCACTTCA+TGG + chr3_2:54471485-54471504 Msa0411870:CDS 35.0%
TGGCAAAAAACCCAAATGAA+TGG + chr3_2:54471505-54471524 Msa0411870:CDS 35.0%
CAAAAAACCCAAATGAATGG+TGG + chr3_2:54471508-54471527 Msa0411870:CDS 35.0%
TGGTTCAAGTGAAATTGGTT+AGG + chr3_2:54471602-54471621 Msa0411870:CDS 35.0%
ATTCAGGAATTGAGGATGAA+AGG + chr3_2:54471688-54471707 Msa0411870:CDS 35.0%
TTCAGGAATTGAGGATGAAA+GGG + chr3_2:54471689-54471708 Msa0411870:CDS 35.0%
TCTAAAGAGTTGCAAGTGAA+TGG + chr3_2:54471727-54471746 Msa0411870:intron 35.0%
CTAAAGAGTTGCAAGTGAAT+GGG + chr3_2:54471728-54471747 Msa0411870:intron 35.0%
!! GTTCGAGTGTTGAGATTAAA+TGG + chr3_2:54471764-54471783 Msa0411870:intron 35.0%
TGTTGAGATTAAATGGACAG+TGG + chr3_2:54471771-54471790 Msa0411870:intron 35.0%
TTAAATGGACAGTGGTTACT+TGG + chr3_2:54471779-54471798 Msa0411870:intron 35.0%
CAAACCACATAGAACAAACT+TGG - chr3_2:54471866-54471885 None:intergenic 35.0%
GACTCATATCGTGATTTCAA+CGG - chr3_2:54472108-54472127 None:intergenic 35.0%
ACGATATGAGTCATTTGATC+TGG + chr3_2:54472116-54472135 Msa0411870:CDS 35.0%
!!! CTAACTTGCTCCTTTTGTAT+TGG + chr3_2:54472261-54472280 Msa0411870:three_prime_UTR 35.0%
CTCTCTTAAATCTACTCACA+AGG - chr3_2:54472297-54472316 None:intergenic 35.0%
TAGAATTGAGTGCACATATC+AGG + chr3_2:54472350-54472369 Msa0411870:three_prime_UTR 35.0%
GATGATGTTCTTGGTCTACT+GGG - chr3_2:54471231-54471250 None:intergenic 40.0%
! TGATGATGTTCTTGGTCTAC+TGG - chr3_2:54471232-54471251 None:intergenic 40.0%
!! GCCAAAGCTTTAAGAGTAAG+AGG - chr3_2:54471348-54471367 None:intergenic 40.0%
AAAACCCAAATGAATGGTGG+TGG + chr3_2:54471511-54471530 Msa0411870:CDS 40.0%
! GTGGTGGAAGTTGTAAGATA+TGG + chr3_2:54471530-54471549 Msa0411870:CDS 40.0%
GTGGAAGTTGTAAGATATGG+TGG + chr3_2:54471533-54471552 Msa0411870:CDS 40.0%
!! TGGTTAGGTATTGGAAAGTG+TGG + chr3_2:54471617-54471636 Msa0411870:CDS 40.0%
!! GGTTAGGTATTGGAAAGTGT+GGG + chr3_2:54471618-54471637 Msa0411870:CDS 40.0%
AAGTGTGGGAACAGTTACAA+AGG + chr3_2:54471632-54471651 Msa0411870:CDS 40.0%
AGTGTGGGAACAGTTACAAA+GGG + chr3_2:54471633-54471652 Msa0411870:CDS 40.0%
! AGGAATGATAGTAGAGGTGT+TGG + chr3_2:54471658-54471677 Msa0411870:CDS 40.0%
!! GGAATGATAGTAGAGGTGTT+GGG + chr3_2:54471659-54471678 Msa0411870:CDS 40.0%
TCAGGAATTGAGGATGAAAG+GGG + chr3_2:54471690-54471709 Msa0411870:CDS 40.0%
CATAGAACAAACTTGGAAGC+AGG - chr3_2:54471859-54471878 None:intergenic 40.0%
! GCTTCCAAGTTTGTTCTATG+TGG + chr3_2:54471859-54471878 Msa0411870:CDS 40.0%
CCAACATTGCTTCATTCCTT+TGG - chr3_2:54472032-54472051 None:intergenic 40.0%
CCAAAGGAATGAAGCAATGT+TGG + chr3_2:54472029-54472048 Msa0411870:CDS 40.0%
! TCCGATTGATTTACTGCACT+TGG + chr3_2:54472052-54472071 Msa0411870:CDS 40.0%
GCCAAGTGCAGTAAATCAAT+CGG - chr3_2:54472056-54472075 None:intergenic 40.0%
TTGATTTACTGCACTTGGCT+CGG + chr3_2:54472057-54472076 Msa0411870:CDS 40.0%
CTAGCTAGTAATTGCGAAGA+CGG + chr3_2:54472141-54472160 Msa0411870:CDS 40.0%
TAGCTAGTAATTGCGAAGAC+GGG + chr3_2:54472142-54472161 Msa0411870:CDS 40.0%
TTACATGGATCATCCGTCAA+AGG - chr3_2:54472420-54472439 None:intergenic 40.0%
GATGATGTTCTTGGTCTACT+GGG - chr3_2:54471231-54471250 None:intergenic 40.0%
! TGATGATGTTCTTGGTCTAC+TGG - chr3_2:54471232-54471251 None:intergenic 40.0%
!! GCCAAAGCTTTAAGAGTAAG+AGG - chr3_2:54471348-54471367 None:intergenic 40.0%
AAAACCCAAATGAATGGTGG+TGG + chr3_2:54471511-54471530 Msa0411870:CDS 40.0%
! GTGGTGGAAGTTGTAAGATA+TGG + chr3_2:54471530-54471549 Msa0411870:CDS 40.0%
GTGGAAGTTGTAAGATATGG+TGG + chr3_2:54471533-54471552 Msa0411870:CDS 40.0%
!! TGGTTAGGTATTGGAAAGTG+TGG + chr3_2:54471617-54471636 Msa0411870:CDS 40.0%
!! GGTTAGGTATTGGAAAGTGT+GGG + chr3_2:54471618-54471637 Msa0411870:CDS 40.0%
AAGTGTGGGAACAGTTACAA+AGG + chr3_2:54471632-54471651 Msa0411870:CDS 40.0%
AGTGTGGGAACAGTTACAAA+GGG + chr3_2:54471633-54471652 Msa0411870:CDS 40.0%
! AGGAATGATAGTAGAGGTGT+TGG + chr3_2:54471658-54471677 Msa0411870:CDS 40.0%
!! GGAATGATAGTAGAGGTGTT+GGG + chr3_2:54471659-54471678 Msa0411870:CDS 40.0%
TCAGGAATTGAGGATGAAAG+GGG + chr3_2:54471690-54471709 Msa0411870:CDS 40.0%
CATAGAACAAACTTGGAAGC+AGG - chr3_2:54471859-54471878 None:intergenic 40.0%
! GCTTCCAAGTTTGTTCTATG+TGG + chr3_2:54471859-54471878 Msa0411870:CDS 40.0%
CCAACATTGCTTCATTCCTT+TGG - chr3_2:54472032-54472051 None:intergenic 40.0%
CCAAAGGAATGAAGCAATGT+TGG + chr3_2:54472029-54472048 Msa0411870:CDS 40.0%
! TCCGATTGATTTACTGCACT+TGG + chr3_2:54472052-54472071 Msa0411870:CDS 40.0%
GCCAAGTGCAGTAAATCAAT+CGG - chr3_2:54472056-54472075 None:intergenic 40.0%
TTGATTTACTGCACTTGGCT+CGG + chr3_2:54472057-54472076 Msa0411870:CDS 40.0%
CTAGCTAGTAATTGCGAAGA+CGG + chr3_2:54472141-54472160 Msa0411870:CDS 40.0%
TAGCTAGTAATTGCGAAGAC+GGG + chr3_2:54472142-54472161 Msa0411870:CDS 40.0%
TTACATGGATCATCCGTCAA+AGG - chr3_2:54472420-54472439 None:intergenic 40.0%
CAGAGATTTCGGTTAAAGGG+TGG - chr3_2:54471312-54471331 None:intergenic 45.0%
ACCGAAATCTCTGAATCACC+CGG + chr3_2:54471319-54471338 Msa0411870:CDS 45.0%
!! TTTAAGAGTAAGAGGTGCCC+GGG - chr3_2:54471340-54471359 None:intergenic 45.0%
!! CTTTAAGAGTAAGAGGTGCC+CGG - chr3_2:54471341-54471360 None:intergenic 45.0%
! GGAGTGTTAGGAAAAAGCCA+TGG - chr3_2:54471474-54471493 None:intergenic 45.0%
TCCACCACCACCATTCATTT+GGG - chr3_2:54471518-54471537 None:intergenic 45.0%
TTCCACCACCACCATTCATT+TGG - chr3_2:54471519-54471538 None:intergenic 45.0%
AGGGAGAGGAATGATAGTAG+AGG + chr3_2:54471652-54471671 Msa0411870:CDS 45.0%
!! ATGATAGTAGAGGTGTTGGG+AGG + chr3_2:54471662-54471681 Msa0411870:CDS 45.0%
! GGAGGTGTATTCAGGAATTG+AGG + chr3_2:54471680-54471699 Msa0411870:CDS 45.0%
!! TTTTTCAGCCTCTCTGCCAA+AGG + chr3_2:54472013-54472032 Msa0411870:intron 45.0%
! GATCAATGGACTTCGGATCA+TGG + chr3_2:54472178-54472197 Msa0411870:three_prime_UTR 45.0%
TTGAGTGCACATATCAGGAG+AGG + chr3_2:54472355-54472374 Msa0411870:three_prime_UTR 45.0%
CAGAGATTTCGGTTAAAGGG+TGG - chr3_2:54471312-54471331 None:intergenic 45.0%
ACCGAAATCTCTGAATCACC+CGG + chr3_2:54471319-54471338 Msa0411870:CDS 45.0%
!! TTTAAGAGTAAGAGGTGCCC+GGG - chr3_2:54471340-54471359 None:intergenic 45.0%
!! CTTTAAGAGTAAGAGGTGCC+CGG - chr3_2:54471341-54471360 None:intergenic 45.0%
! GGAGTGTTAGGAAAAAGCCA+TGG - chr3_2:54471474-54471493 None:intergenic 45.0%
TCCACCACCACCATTCATTT+GGG - chr3_2:54471518-54471537 None:intergenic 45.0%
TTCCACCACCACCATTCATT+TGG - chr3_2:54471519-54471538 None:intergenic 45.0%
AGGGAGAGGAATGATAGTAG+AGG + chr3_2:54471652-54471671 Msa0411870:CDS 45.0%
!! ATGATAGTAGAGGTGTTGGG+AGG + chr3_2:54471662-54471681 Msa0411870:CDS 45.0%
! GGAGGTGTATTCAGGAATTG+AGG + chr3_2:54471680-54471699 Msa0411870:CDS 45.0%
!! TTTTTCAGCCTCTCTGCCAA+AGG + chr3_2:54472013-54472032 Msa0411870:intron 45.0%
! GATCAATGGACTTCGGATCA+TGG + chr3_2:54472178-54472197 Msa0411870:three_prime_UTR 45.0%
TTGAGTGCACATATCAGGAG+AGG + chr3_2:54472355-54472374 Msa0411870:three_prime_UTR 45.0%
CCCGGGTGATTCAGAGATTT+CGG - chr3_2:54471323-54471342 None:intergenic 50.0%
CCGAAATCTCTGAATCACCC+GGG + chr3_2:54471320-54471339 Msa0411870:CDS 50.0%
!! ACCCAAATGAATGGTGGTGG+TGG + chr3_2:54471514-54471533 Msa0411870:CDS 50.0%
GGGAACAGTTACAAAGGGAG+AGG + chr3_2:54471638-54471657 Msa0411870:CDS 50.0%
!! AGGTGTTGGGAGGTGTATTC+AGG + chr3_2:54471672-54471691 Msa0411870:CDS 50.0%
GCTTCATTCCTTTGGCAGAG+AGG - chr3_2:54472024-54472043 None:intergenic 50.0%
CCCGGGTGATTCAGAGATTT+CGG - chr3_2:54471323-54471342 None:intergenic 50.0%
CCGAAATCTCTGAATCACCC+GGG + chr3_2:54471320-54471339 Msa0411870:CDS 50.0%
!! ACCCAAATGAATGGTGGTGG+TGG + chr3_2:54471514-54471533 Msa0411870:CDS 50.0%
GGGAACAGTTACAAAGGGAG+AGG + chr3_2:54471638-54471657 Msa0411870:CDS 50.0%
!! AGGTGTTGGGAGGTGTATTC+AGG + chr3_2:54471672-54471691 Msa0411870:CDS 50.0%
GCTTCATTCCTTTGGCAGAG+AGG - chr3_2:54472024-54472043 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
chr3_2 gene 54471190 54472462 54471190 ID=Msa0411870;Name=Msa0411870
chr3_2 mRNA 54471190 54472462 54471190 ID=Msa0411870-mRNA-1;Parent=Msa0411870;Name=Msa0411870-mRNA-1;_AED=0.10;_eAED=0.16;_QI=0|0|0.33|1|1|1|3|289|254
chr3_2 exon 54471190 54471741 54471190 ID=Msa0411870-mRNA-1:exon:11051;Parent=Msa0411870-mRNA-1
chr3_2 exon 54471845 54471904 54471845 ID=Msa0411870-mRNA-1:exon:11052;Parent=Msa0411870-mRNA-1
chr3_2 exon 54472021 54472462 54472021 ID=Msa0411870-mRNA-1:exon:11053;Parent=Msa0411870-mRNA-1
chr3_2 CDS 54471190 54471741 54471190 ID=Msa0411870-mRNA-1:cds;Parent=Msa0411870-mRNA-1
chr3_2 CDS 54471845 54471904 54471845 ID=Msa0411870-mRNA-1:cds;Parent=Msa0411870-mRNA-1
chr3_2 CDS 54472021 54472173 54472021 ID=Msa0411870-mRNA-1:cds;Parent=Msa0411870-mRNA-1
chr3_2 three_prime_UTR 54472174 54472462 54472174 ID=Msa0411870-mRNA-1:three_prime_utr;Parent=Msa0411870-mRNA-1
Gene Sequence

>Msa0411870

ATGGCTGAAACAAACAACACTCATCACATTGTTGAAATCCCAGTAGACCAAGAACATCATCAAAACAACAACAACAACAAAGCTTTGTTATGTTCTAATATGTTCGACGCAATCGACGACCACCCTTTAACCGAAATCTCTGAATCACCCGGGCACCTCTTACTCTTAAAGCTTTGGCAAAGAGAAGAAAATCTCTTCGCCAAACGCATTTCAAGAAAAGAAACAAGAATGGATTCAATCAAATCCGAACTTTTTCAACTCACTTCATTCTTCTTCATTTTCCATGGCTTTTTCCTAACACTCCTTTTCACTTCATGGCAAAAAACCCAAATGAATGGTGGTGGTGGAAGTTGTAAGATATGGTGGATACCATCATTGATTTCAGTTTCAACTTCACTTGTGTTTGTGTTTTTGGTTCAAGTGAAATTGGTTAGGTATTGGAAAGTGTGGGAACAGTTACAAAGGGAGAGGAATGATAGTAGAGGTGTTGGGAGGTGTATTCAGGAATTGAGGATGAAAGGGGCTAGTTTTGATTTGTCTAAAGAGTTGCAAGTTTGGCTTTTCCTGCTTCCAAGTTTGTTCTATGTGGTTTGTGATCAAGATTATGATGATCCTCTCTGCCAAAGGAATGAAGCAATGTTGGTCCGATTGATTTACTGCACTTGGCTCGGCTCGATTGTTGATGAAGATTATAGACCGTTGAAATCACGATATGAGTCATTTGATCTGGATCTAGCTAGTAATTGCGAAGACGGGAATTGTTGA
Protein sequence

>Msa0411870

MAETNNTHHIVEIPVDQEHHQNNNNNKALLCSNMFDAIDDHPLTEISESPGHLLLLKLWQREENLFAKRISRKETRMDSIKSELFQLTSFFFIFHGFFLTLLFTSWQKTQMNGGGGSCKIWWIPSLISVSTSLVFVFLVQVKLVRYWKVWEQLQRERNDSRGVGRCIQELRMKGASFDLSKELQVWLFLLPSLFYVVCDQDYDDPLCQRNEAMLVRLIYCTWLGSIVDEDYRPLKSRYESFDLDLASNCEDGNC*