AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0437150

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0437150 MtrunA17_Chr7g0263281 98.108 370 7 0 1 370 1 370 0.0 753
Msa0437150 MtrunA17_Chr1g0181901 70.720 403 84 3 1 369 1 403 0.0 565
Msa0437150 MtrunA17_Chr1g0213351 66.954 348 111 2 5 348 13 360 4.01e-174 489
Msa0437150 MtrunA17_Chr7g0250061 64.754 366 125 2 4 365 6 371 1.03e-168 476
Msa0437150 MtrunA17_Chr1g0211251 63.115 366 131 2 4 365 11 376 1.12e-166 471
Msa0437150 MtrunA17_Chr8g0371321 62.931 348 128 1 17 363 25 372 1.45e-164 466
Msa0437150 MtrunA17_Chr2g0294291 56.438 365 145 3 5 363 12 368 1.29e-149 427
Msa0437150 MtrunA17_Chr1g0175381 54.268 328 141 3 22 349 38 356 2.78e-127 370
Msa0437150 MtrunA17_Chr1g0207571 54.079 331 142 2 18 346 16 338 1.26e-123 361
Msa0437150 MtrunA17_Chr3g0089711 46.154 325 158 6 29 346 33 347 1.07e-96 292
Msa0437150 MtrunA17_Chr3g0089721 46.154 325 158 6 29 346 33 347 2.20e-96 291
Msa0437150 MtrunA17_Chr7g0256751 46.462 325 157 6 29 346 34 348 5.96e-91 280
Msa0437150 MtrunA17_Chr7g0256771 45.221 272 138 4 29 298 35 297 1.77e-75 236
Msa0437150 MtrunA17_Chr3g0089691 45.318 267 126 5 86 346 9 261 1.76e-72 228
Msa0437150 MtrunA17_Chr4g0063111 35.952 331 193 6 22 348 34 349 1.17e-61 206
Msa0437150 MtrunA17_Chr4g0063051 33.618 351 188 12 3 348 1 311 8.61e-47 162
Msa0437150 MtrunA17_Chr2g0295991 31.962 316 169 7 71 345 166 476 2.75e-43 156
Msa0437150 MtrunA17_Chr7g0238501 29.530 298 181 6 71 349 263 550 2.07e-39 147
Msa0437150 MtrunA17_Chr4g0067301 29.483 329 173 7 71 345 164 487 1.05e-38 144
Msa0437150 MtrunA17_Chr4g0039061 30.435 299 176 7 70 349 201 486 2.20e-37 140
Msa0437150 MtrunA17_Chr7g0256761 41.117 197 93 5 159 350 7 185 4.71e-36 130
Msa0437150 MtrunA17_Chr4g0031521 30.466 279 152 6 108 349 426 699 1.83e-34 134
Msa0437150 MtrunA17_Chr1g0193691 29.236 301 148 5 109 349 592 887 2.09e-33 131
Msa0437150 MtrunA17_Chr4g0054811 29.450 309 159 6 102 356 475 778 1.39e-32 129
Msa0437150 MtrunA17_Chr3g0105371 30.686 277 136 7 70 345 117 338 5.70e-32 123
Msa0437150 MtrunA17_Chr5g0430481 34.529 223 104 6 70 291 153 334 1.10e-31 123
Msa0437150 MtrunA17_Chr3g0105351 29.964 277 138 7 70 345 93 314 1.77e-30 118
Msa0437150 MtrunA17_Chr1g0212961 28.710 310 148 9 47 353 106 345 1.32e-26 108
Msa0437150 MtrunA17_Chr1g0150451 30.627 271 138 9 47 313 88 312 4.07e-24 101
Msa0437150 MtrunA17_Chr4g0050381 30.877 285 144 12 35 314 30 266 4.90e-24 100
Msa0437150 MtrunA17_Chr2g0317021 30.576 278 128 10 71 345 117 332 5.49e-24 100
Msa0437150 MtrunA17_Chr3g0126511 30.870 230 112 6 66 291 120 306 7.36e-24 101
Msa0437150 MtrunA17_Chr5g0426141 31.034 319 144 13 31 343 31 279 7.72e-24 99.8
Msa0437150 MtrunA17_Chr3g0136711 29.000 300 144 11 47 343 45 278 1.66e-23 99.0
Msa0437150 MtrunA17_Chr5g0407161 29.371 286 129 9 71 346 124 346 1.89e-23 100
Msa0437150 MtrunA17_Chr4g0070871 29.297 256 122 7 54 291 231 445 9.13e-22 96.7
Msa0437150 MtrunA17_Chr2g0279151 29.078 282 137 9 64 343 58 278 1.54e-21 93.2
Msa0437150 MtrunA17_Chr4g0062951 32.065 184 115 6 105 279 1 183 1.95e-21 90.5
Msa0437150 MtrunA17_Chr3g0115851 28.797 316 155 13 31 343 31 279 1.21e-20 90.9
Msa0437150 MtrunA17_Chr8g0360351 27.187 320 164 12 30 346 29 282 1.33e-20 90.5
Msa0437150 MtrunA17_Chr2g0293811 29.825 285 141 9 70 346 99 332 2.31e-20 91.3
Msa0437150 MtrunA17_Chr1g0171571 29.537 281 140 10 72 347 419 646 4.17e-20 92.0
Msa0437150 MtrunA17_Chr3g0144111 28.223 287 116 9 69 347 25 229 4.39e-20 88.6
Msa0437150 MtrunA17_Chr3g0138281 28.421 285 143 11 66 345 117 345 2.32e-19 88.6
Msa0437150 MtrunA17_Chr4g0065141 28.777 278 142 9 70 343 123 348 3.17e-19 88.2
Msa0437150 MtrunA17_Chr3g0135961 28.197 305 160 12 60 345 275 539 3.74e-19 89.0
Msa0437150 MtrunA17_Chr1g0158781 27.726 321 176 11 35 349 113 383 1.27e-18 86.7
Msa0437150 MtrunA17_Chr5g0435721 27.586 232 129 5 68 292 220 419 8.33e-18 84.7
Msa0437150 MtrunA17_Chr3g0112291 25.000 296 158 10 71 345 123 375 9.24e-18 84.0
Msa0437150 MtrunA17_Chr4g0051661 28.926 242 125 10 71 308 183 381 1.60e-17 83.2
Msa0437150 MtrunA17_Chr1g0150781 27.419 310 149 12 67 348 285 546 2.03e-17 83.6
Msa0437150 MtrunA17_Chr7g0223641 30.493 223 110 7 135 345 7 196 3.00e-17 79.7
Msa0437150 MtrunA17_Chr1g0194191 26.573 286 149 11 70 346 117 350 1.28e-16 80.5
Msa0437150 MtrunA17_Chr3g0136811 25.614 285 138 10 52 292 33 287 2.13e-16 79.7
Msa0437150 MtrunA17_Chr1g0150071 26.592 267 122 10 70 292 51 287 8.89e-16 77.8
Msa0437150 MtrunA17_Chr2g0295401 28.873 284 148 11 71 326 92 349 1.34e-15 77.4
Msa0437150 MtrunA17_Chr7g0221691 32.075 159 90 5 152 309 245 386 8.28e-15 75.5
Msa0437150 MtrunA17_Chr4g0004971 26.154 325 160 14 63 351 69 349 1.18e-13 71.6
Msa0437150 MtrunA17_Chr8g0342431 31.928 166 83 5 152 310 210 352 2.47e-13 70.9
Msa0437150 MtrunA17_Chr3g0127091 26.482 253 138 10 70 297 88 317 9.87e-13 68.9
Msa0437150 MtrunA17_Chr5g0396731 24.399 291 165 7 71 349 107 354 1.00e-12 68.6
Msa0437150 MtrunA17_Chr1g0151781 27.170 265 136 10 56 292 53 288 3.03e-12 67.0
Msa0437150 MtrunA17_Chr1g0192421 27.465 284 152 13 71 326 89 346 6.77e-12 66.2
Msa0437150 MtrunA17_Chr4g0067711 30.000 200 104 8 138 326 174 348 7.50e-12 65.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0437150 AT3G12620.2 66.304 368 121 1 1 365 10 377 0.0 511
Msa0437150 AT3G12620.1 66.304 368 121 1 1 365 10 377 0.0 511
Msa0437150 AT3G55050.1 64.481 366 121 2 5 365 13 374 7.25e-177 497
Msa0437150 AT3G55050.2 64.481 366 121 2 5 365 13 374 7.25e-177 497
Msa0437150 AT3G51370.1 65.994 347 116 1 19 365 27 371 6.11e-174 489
Msa0437150 AT3G51370.3 65.994 347 116 1 19 365 27 371 6.11e-174 489
Msa0437150 AT5G66080.1 64.857 350 121 2 18 365 28 377 1.49e-168 476
Msa0437150 AT4G38520.2 62.178 349 131 1 16 363 26 374 1.48e-165 469
Msa0437150 AT4G38520.1 62.178 349 131 1 16 363 26 374 1.48e-165 469
Msa0437150 AT5G02760.1 62.264 371 136 3 1 370 1 368 8.96e-162 458
Msa0437150 AT3G17090.1 57.270 337 132 4 24 358 45 371 1.23e-135 392
Msa0437150 AT3G51370.2 64.130 276 97 1 90 365 13 286 2.30e-131 378
Msa0437150 AT5G66080.2 65.428 269 92 1 98 365 5 273 1.18e-127 368
Msa0437150 AT4G33920.1 51.862 349 149 3 22 359 20 360 1.51e-124 364
Msa0437150 AT5G06750.3 49.856 347 163 2 24 367 43 381 1.22e-119 352
Msa0437150 AT5G06750.1 49.856 347 163 2 24 367 43 381 1.22e-119 352
Msa0437150 AT3G17090.2 55.797 276 110 4 24 297 45 310 1.50e-106 316
Msa0437150 AT4G33920.2 53.480 273 116 2 98 359 5 277 1.65e-98 295
Msa0437150 AT5G06750.2 51.852 270 127 1 101 367 30 299 7.76e-94 283
Msa0437150 AT4G33920.3 54.656 247 101 2 124 359 1 247 3.65e-91 275
Msa0437150 AT3G16560.1 31.310 313 170 7 71 345 175 480 2.00e-42 154
Msa0437150 AT3G16560.4 31.310 313 170 7 71 345 175 480 2.00e-42 154
Msa0437150 AT3G16560.2 31.310 313 170 7 71 345 175 480 2.00e-42 154
Msa0437150 AT1G07160.1 34.520 281 129 7 67 345 149 376 7.19e-38 140
Msa0437150 AT5G02400.1 31.884 276 149 5 108 349 399 669 3.93e-37 142
Msa0437150 AT2G46920.3 28.713 303 150 5 108 349 542 839 2.35e-35 137
Msa0437150 AT2G46920.1 28.713 303 150 5 108 349 542 839 2.35e-35 137
Msa0437150 AT2G46920.2 28.713 303 150 5 108 349 542 839 2.35e-35 137
Msa0437150 AT2G46920.4 28.713 303 150 5 108 349 542 839 2.35e-35 137
Msa0437150 AT3G09400.1 30.037 273 151 6 108 349 382 645 8.67e-35 135
Msa0437150 AT1G07630.1 29.032 279 156 5 108 349 384 657 1.38e-34 135
Msa0437150 AT2G28890.1 29.391 279 155 5 108 349 376 649 1.88e-34 134
Msa0437150 AT2G40180.1 31.683 303 136 10 58 345 139 385 7.09e-34 129
Msa0437150 AT3G16560.3 30.742 283 151 7 71 315 175 450 2.33e-33 129
Msa0437150 AT2G35350.1 28.383 303 161 6 102 358 486 778 4.06e-33 131
Msa0437150 AT2G30020.1 32.975 279 129 9 70 345 169 392 4.02e-30 119
Msa0437150 AT1G67820.2 34.222 225 105 5 70 292 114 297 1.19e-28 115
Msa0437150 AT1G67820.1 34.222 225 105 5 70 292 150 333 1.63e-28 115
Msa0437150 AT2G33700.1 32.877 292 117 12 69 347 121 346 1.54e-25 106
Msa0437150 AT3G09400.2 28.870 239 130 6 108 315 382 611 1.57e-25 108
Msa0437150 AT4G31750.2 30.147 272 139 9 47 313 44 269 8.40e-25 103
Msa0437150 AT4G31750.1 30.147 272 139 9 47 313 44 269 1.02e-24 103
Msa0437150 AT4G31750.3 30.147 272 139 9 47 313 44 269 1.02e-24 103
Msa0437150 AT3G51470.2 28.763 299 137 11 62 348 52 286 3.04e-24 102
Msa0437150 AT3G51470.1 28.763 299 137 11 62 348 96 330 6.10e-24 101
Msa0437150 AT1G43900.1 32.526 289 121 11 62 343 145 366 1.12e-23 101
Msa0437150 AT5G10740.1 30.556 288 146 11 31 313 30 268 1.42e-23 100
Msa0437150 AT5G10740.2 30.556 288 146 11 31 313 30 268 1.42e-23 100
Msa0437150 AT1G78200.1 29.393 313 152 12 35 343 33 280 7.25e-23 97.4
Msa0437150 AT1G78200.3 29.393 313 152 12 35 343 33 280 7.25e-23 97.4
Msa0437150 AT1G78200.2 29.393 313 152 12 35 343 33 280 7.25e-23 97.4
Msa0437150 AT5G53140.1 26.756 299 146 9 71 359 132 367 4.07e-22 97.4
Msa0437150 AT5G53140.2 26.756 299 146 9 71 359 132 367 4.07e-22 97.4
Msa0437150 AT1G78200.5 29.021 286 139 10 62 343 10 235 5.66e-22 94.0
Msa0437150 AT1G78200.4 29.021 286 139 10 62 343 10 235 5.66e-22 94.0
Msa0437150 AT2G25620.1 28.165 316 165 13 43 348 96 359 7.41e-22 96.3
Msa0437150 AT2G25620.2 28.165 316 165 13 43 348 96 359 7.41e-22 96.3
Msa0437150 AT4G28400.3 30.315 254 129 9 66 314 40 250 1.84e-21 92.8
Msa0437150 AT5G24940.1 29.825 285 146 11 31 310 30 265 2.98e-21 95.1
Msa0437150 AT4G28400.1 29.371 286 133 10 66 346 62 283 3.36e-21 92.8
Msa0437150 AT2G20630.1 29.242 277 129 10 71 343 63 276 8.70e-21 91.7
Msa0437150 AT2G20630.2 28.986 276 129 10 71 342 63 275 4.81e-20 89.7
Msa0437150 AT1G07430.1 28.696 230 124 5 71 292 157 354 7.36e-20 90.9
Msa0437150 AT1G34750.2 28.526 312 152 12 38 345 37 281 2.23e-19 87.4
Msa0437150 AT1G34750.1 28.526 312 152 12 38 345 37 281 2.23e-19 87.4
Msa0437150 AT1G34750.3 28.526 312 152 12 38 345 37 281 2.23e-19 87.4
Msa0437150 AT2G29380.1 28.571 301 162 10 57 345 101 360 2.61e-19 88.6
Msa0437150 AT1G48040.1 30.088 339 156 14 39 359 81 356 2.65e-19 89.0
Msa0437150 AT3G17250.1 28.571 273 140 10 62 314 142 379 3.84e-19 88.6
Msa0437150 AT1G22280.3 28.349 321 160 13 29 343 28 284 5.20e-19 86.7
Msa0437150 AT4G08260.1 27.698 278 112 10 70 345 18 208 6.65e-19 84.7
Msa0437150 AT4G26080.1 26.087 322 184 10 54 361 153 434 1.66e-18 87.0
Msa0437150 AT1G22280.1 28.481 316 160 12 29 343 28 278 1.92e-18 84.7
Msa0437150 AT3G62260.3 30.242 248 134 9 71 309 27 244 2.00e-18 85.1
Msa0437150 AT3G15260.2 29.603 277 128 11 71 343 73 286 2.94e-18 84.3
Msa0437150 AT3G15260.1 29.603 277 128 11 71 343 73 286 2.94e-18 84.3
Msa0437150 AT2G40860.1 28.470 281 144 8 71 347 423 650 3.27e-18 86.7
Msa0437150 AT2G40860.4 28.470 281 144 8 71 347 325 552 3.62e-18 86.3
Msa0437150 AT5G51760.2 27.083 240 124 8 71 291 114 321 4.82e-18 85.1
Msa0437150 AT5G51760.1 27.083 240 124 8 71 291 144 351 6.12e-18 85.1
Msa0437150 AT3G62260.1 30.242 248 134 9 71 309 116 333 7.20e-18 84.7
Msa0437150 AT3G62260.2 30.242 248 134 9 71 309 117 334 7.26e-18 84.7
Msa0437150 AT2G34740.1 28.938 273 148 10 44 314 96 324 1.11e-17 83.6
Msa0437150 AT2G25070.1 25.887 282 141 8 52 292 33 287 2.20e-17 82.8
Msa0437150 AT2G25070.2 25.887 282 141 8 52 292 33 287 2.20e-17 82.8
Msa0437150 AT3G16800.2 28.428 299 153 12 70 347 92 350 2.34e-17 82.8
Msa0437150 AT3G16800.1 28.428 299 153 12 70 347 92 350 2.34e-17 82.8
Msa0437150 AT3G16800.3 28.428 299 153 12 70 347 92 350 2.34e-17 82.8
Msa0437150 AT5G59220.1 28.384 229 123 6 71 292 148 342 6.42e-17 82.0
Msa0437150 AT3G16800.4 29.832 238 117 9 122 347 56 255 8.71e-17 79.7
Msa0437150 AT3G16800.6 29.832 238 117 9 122 347 56 255 8.71e-17 79.7
Msa0437150 AT2G40860.2 28.226 248 137 7 71 311 423 636 2.12e-16 81.3
Msa0437150 AT1G72770.4 27.308 260 121 5 60 291 227 446 2.46e-16 80.9
Msa0437150 AT1G72770.1 27.308 260 121 5 60 291 227 446 2.46e-16 80.9
Msa0437150 AT1G72770.3 27.308 260 121 5 60 291 227 446 2.46e-16 80.9
Msa0437150 AT2G34740.2 29.289 239 124 9 44 281 110 304 4.30e-16 79.0
Msa0437150 AT1G17550.1 26.603 312 151 12 71 348 237 504 4.41e-16 80.1
Msa0437150 AT1G17550.2 26.603 312 151 12 71 348 237 504 4.41e-16 80.1
Msa0437150 AT2G34740.3 29.289 239 124 9 44 281 92 286 4.41e-16 78.6
Msa0437150 AT1G18030.1 28.736 261 129 9 72 311 110 334 2.08e-15 77.0
Msa0437150 AT5G27930.1 28.000 300 152 14 71 344 92 353 4.85e-15 76.3
Msa0437150 AT5G27930.2 28.000 300 152 14 71 344 92 353 4.85e-15 76.3
Msa0437150 AT3G11410.1 24.314 255 134 7 71 308 137 349 5.42e-15 76.3
Msa0437150 AT1G34750.4 27.823 248 128 9 38 281 37 237 5.08e-14 71.6
Msa0437150 AT3G05640.2 27.957 279 152 13 71 326 93 345 5.14e-14 72.8
Msa0437150 AT3G05640.1 27.957 279 152 13 71 326 93 345 5.14e-14 72.8
Msa0437150 AT3G05640.3 27.957 279 152 13 71 326 93 345 5.14e-14 72.8
Msa0437150 AT5G26010.1 26.629 353 200 13 11 348 19 327 6.31e-14 72.4
Msa0437150 AT4G32950.1 26.014 296 167 12 64 348 65 319 1.44e-12 68.6

Find 116 sgRNAs with CRISPR-Local

Find 209 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACCTATGAGTTCTGATTATT+TGG 0.094977 3_2:-91125410 Msa0437150:CDS
TGCTATTGTTCTTGTCTTCT+TGG 0.118706 3_2:+91125648 None:intergenic
TCACTCCTTGTTCAATCTTT+TGG 0.144007 3_2:+91123559 None:intergenic
TCTTTGCTTCTGACGGTTTA+TGG 0.156191 3_2:-91123797 Msa0437150:CDS
CAGTCTCCCACAGTAGTTTA+TGG 0.181885 3_2:-91123321 Msa0437150:three_prime_UTR
AGTAGATAAGAGAAGGATTT+TGG 0.195079 3_2:+91126286 None:intergenic
GATTTGGGAAACCATCTTTA+TGG 0.196034 3_2:-91125499 Msa0437150:CDS
GGTCCTCAAGGAACCTTTAT+AGG 0.223798 3_2:-91125388 Msa0437150:CDS
CCCACAGTAGTTTATGGTTA+TGG 0.252793 3_2:-91123315 Msa0437150:three_prime_UTR
TGAGAGCATACTCTTGAAAT+TGG 0.253266 3_2:+91125306 None:intergenic
AGGTTTCTTTCATTCTTTCT+AGG 0.264590 3_2:+91123419 None:intergenic
ACTGAGAATTGGCTTGAAAA+AGG 0.264897 3_2:+91123872 None:intergenic
CTAAGTGTGTTCCTTTAATA+TGG 0.271025 3_2:-91125922 Msa0437150:five_prime_UTR
CTTTGCTTCTGACGGTTTAT+GGG 0.274010 3_2:-91123796 Msa0437150:CDS
GAGCTATTATCAATCAGTTT+TGG 0.288253 3_2:+91123489 None:intergenic
ATTGTTATGGTACAAGGATT+TGG 0.289189 3_2:-91125515 Msa0437150:CDS
TCAGAGGTGTGCCATATTAA+AGG 0.291940 3_2:+91125911 None:intergenic
TGAAGAAAGCAGAATTTAAC+AGG 0.297447 3_2:-91123929 Msa0437150:CDS
CTGAGCATCCATTCAATACT+TGG 0.302446 3_2:-91123394 Msa0437150:three_prime_UTR
TTGTTATGGTACAAGGATTT+GGG 0.311993 3_2:-91125514 Msa0437150:CDS
AAGATGAGAAATTGATCTTC+AGG 0.326898 3_2:+91123816 None:intergenic
TATAGGTATATATATAGATT+TGG 0.334918 3_2:-91125695 Msa0437150:intron
ACCATCATAAACACCTATAA+AGG 0.367844 3_2:+91125375 None:intergenic
AACCACAATCTGTGAATCAT+AGG 0.369310 3_2:+91124564 None:intergenic
CTTAGCTGTAAAAGCACATA+TGG 0.371280 3_2:+91125940 None:intergenic
CCTATGAGTTCTGATTATTT+GGG 0.385684 3_2:-91125409 Msa0437150:CDS
CAACATAAATTTGTGATTGT+GGG 0.387941 3_2:+91123244 None:intergenic
GATGGCTCACTACTGAGAAT+TGG 0.390286 3_2:+91123861 None:intergenic
ATCTGCTGGTACATGTTGTT+TGG 0.408579 3_2:-91124752 Msa0437150:CDS
TTGAAAATTCACCATAAAGA+TGG 0.408650 3_2:+91125488 None:intergenic
AATTCAAGCAAATAGTTCAT+TGG 0.412793 3_2:-91125458 Msa0437150:CDS
TCTTGTTGTATAAAGAAGAT+AGG 0.421011 3_2:-91123362 Msa0437150:three_prime_UTR
GAAGAGTGAGGAAGGGAACA+AGG 0.421093 3_2:-91124666 Msa0437150:CDS
TTTCAGAAAAGGTTATCAAA+AGG 0.431288 3_2:-91124846 Msa0437150:CDS
TGATTGATAATAGCTCTCTA+TGG 0.431364 3_2:-91123482 Msa0437150:CDS
GGAGACTCAAGGGCTGTGTT+AGG 0.432476 3_2:-91124688 Msa0437150:CDS
GGAAAGTTGATGGATTGTTA+TGG 0.432672 3_2:-91125528 Msa0437150:CDS
GATCGTAGCCAACTTGAATC+AGG 0.435753 3_2:-91125433 Msa0437150:CDS
AAGCACATATGGACTCAATA+TGG 0.440468 3_2:+91125951 None:intergenic
TAACGATATTCACAGCTACT+TGG 0.443990 3_2:+91123760 None:intergenic
CTTCAGATATGCATCACCTA+TGG 0.450543 3_2:+91123947 None:intergenic
TCAACATAAATTTGTGATTG+TGG 0.453642 3_2:+91123243 None:intergenic
TGATGGATTGTTATGGTACA+AGG 0.456025 3_2:-91125521 Msa0437150:CDS
GCTGGTACATGTTGTTTGGC+AGG 0.456613 3_2:-91124748 Msa0437150:CDS
TTCAGAAAAGGTTATCAAAA+GGG 0.466769 3_2:-91124845 Msa0437150:CDS
AGATCAAGGGATTTCAGAAA+AGG 0.471446 3_2:-91124857 Msa0437150:CDS
TTTCTCATCTTTGCTTCTGA+CGG 0.472510 3_2:-91123804 Msa0437150:CDS
CACTCTGTTTGGCGTGTGAA+AGG 0.474473 3_2:-91124535 Msa0437150:CDS
CCCAAATAATCAGAACTCAT+AGG 0.475948 3_2:+91125409 None:intergenic
CGGAGGGAGTAGATAAGAGA+AGG 0.477015 3_2:+91126279 None:intergenic
TGTGTTAGGAAGAGTGAGGA+AGG 0.477652 3_2:-91124674 Msa0437150:CDS
AGCTAATAAGCTAAAATATG+AGG 0.478377 3_2:+91123108 None:intergenic
TGCTAAAACCAAGTATTGAA+TGG 0.481415 3_2:+91123386 None:intergenic
AAGATACAAACTAACGTTCA+AGG 0.484502 3_2:-91125870 Msa0437150:five_prime_UTR
TGGTTATGAAACACTCTGTT+TGG 0.490832 3_2:-91124546 Msa0437150:CDS
AAGAGTGAGGAAGGGAACAA+GGG 0.493346 3_2:-91124665 Msa0437150:CDS
TGAGAAATTGATCTTCAGGT+TGG 0.496104 3_2:+91123820 None:intergenic
ATGTGCATTTAAAATTTGCT+TGG 0.501597 3_2:+91123196 None:intergenic
GATGTGAAAGGGAAAGTTGA+TGG 0.502197 3_2:-91125538 Msa0437150:CDS
ACCTTTATAGGTGTTTATGA+TGG 0.502488 3_2:-91125376 Msa0437150:CDS
TTGCAGGTTTCAAGATCCAT+AGG 0.507189 3_2:-91123963 Msa0437150:intron
GGTGGTGATGGAGATGTGAA+AGG 0.507401 3_2:-91125550 Msa0437150:CDS
TGGACTCAATATGGCAGAGT+TGG 0.517378 3_2:+91125960 None:intergenic
CTGCCATCTCTGATTGAAAG+AGG 0.518398 3_2:+91123453 None:intergenic
GAAACGAAGCTTACATTGCG+TGG 0.519390 3_2:+91123729 None:intergenic
CTCTTTCAATCAGAGATGGC+AGG 0.520547 3_2:-91123452 Msa0437150:CDS
TCAACTGTGGAGGGTGGTGA+TGG 0.527360 3_2:-91125562 Msa0437150:CDS
TCCCTATGATTCACAGATTG+TGG 0.527683 3_2:-91124566 Msa0437150:CDS
ATAGGTGTTTATGATGGTCA+TGG 0.527745 3_2:-91125370 Msa0437150:CDS
AAACTGAGAGAGATGATGTT+CGG 0.531123 3_2:-91124597 Msa0437150:CDS
CTGGTACATGTTGTTTGGCA+GGG 0.535327 3_2:-91124747 Msa0437150:CDS
TCAAGCAAATAGTTCATTGG+AGG 0.537174 3_2:-91125455 Msa0437150:CDS
TATCTATGACTATATCATGC+TGG 0.539611 3_2:-91125588 Msa0437150:exon
TTGAAACTTGAAGAGTCTCA+TGG 0.548045 3_2:-91123287 Msa0437150:three_prime_UTR
TCTGATTATTTGGGTCCTCA+AGG 0.548992 3_2:-91125400 Msa0437150:CDS
GCGTGTGAAAGGCATCATAC+AGG 0.551663 3_2:-91124524 Msa0437150:intron
GTTAGTTTGTATCTTGCATG+TGG 0.552758 3_2:+91125878 None:intergenic
TGTCTCTTGTGAAGAAGCAA+TGG 0.558482 3_2:-91124795 Msa0437150:CDS
AGTCTAAACTTCTGTGGCAG+AGG 0.558697 3_2:+91123903 None:intergenic
GTGGTGATGGAGATGTGAAA+GGG 0.558872 3_2:-91125549 Msa0437150:CDS
TTGCAAATGCAGGAGACTCA+AGG 0.563487 3_2:-91124699 Msa0437150:CDS
TCTCCTCTTTCAATCAGAGA+TGG 0.566031 3_2:-91123456 Msa0437150:CDS
TCATAGGTCCTGATTCAAGT+TGG 0.579655 3_2:+91125425 None:intergenic
CAGAGAAAACGAAAGCGTGT+TGG 0.579973 3_2:+91125838 None:intergenic
CAATTACTGTAATATCATCA+TGG 0.580958 3_2:+91123526 None:intergenic
ACCACAATCTGTGAATCATA+GGG 0.581742 3_2:+91124565 None:intergenic
TGCAAATGCAGGAGACTCAA+GGG 0.586962 3_2:-91124698 Msa0437150:CDS
TATTAGCTCAAACTCAAGCA+AGG 0.587242 3_2:-91123093 Msa0437150:three_prime_UTR
CCATAACCATAAACTACTGT+GGG 0.588499 3_2:+91123315 None:intergenic
TGCTGGAAATCAACTGTGGA+GGG 0.596448 3_2:-91125571 Msa0437150:CDS
GCTACTAGTTTCTCATCCCA+AGG 0.598922 3_2:-91125800 Msa0437150:intron
TCAGGTAGTCTAAACTTCTG+TGG 0.603011 3_2:+91123897 None:intergenic
ACATTGTGATCTGATTATGA+AGG 0.605518 3_2:-91123177 Msa0437150:three_prime_UTR
TTTAATATGGCACACCTCTG+AGG 0.608672 3_2:-91125909 Msa0437150:five_prime_UTR
CTAAAATATGAGGCCAATGG+TGG 0.609733 3_2:+91123118 None:intergenic
ATGCTGTATATTGCAAATGC+AGG 0.610667 3_2:-91124709 Msa0437150:CDS
AAGCTAAAATATGAGGCCAA+TGG 0.612731 3_2:+91123115 None:intergenic
AAACCACAGATAGCATCTGC+TGG 0.614223 3_2:-91124766 Msa0437150:CDS
ACCATAACCATAAACTACTG+TGG 0.618055 3_2:+91123314 None:intergenic
GACCTCCAAAAGATTGAACA+AGG 0.625989 3_2:-91123564 Msa0437150:CDS
TTGGCAGGGATAATTTGCAA+TGG 0.628153 3_2:-91124733 Msa0437150:CDS
AAAAGATTGAACAAGGAGTG+AGG 0.629700 3_2:-91123557 Msa0437150:CDS
ATTAGCTCAAACTCAAGCAA+GGG 0.643945 3_2:-91123092 Msa0437150:three_prime_UTR
ATGCTGGAAATCAACTGTGG+AGG 0.664898 3_2:-91125572 Msa0437150:CDS
ATCATGCTGGAAATCAACTG+TGG 0.668033 3_2:-91125575 Msa0437150:CDS
TCATGTAACAGGGAATAGCA+AGG 0.668087 3_2:-91123644 Msa0437150:intron
GTGTTAGGAAGAGTGAGGAA+GGG 0.687678 3_2:-91124673 Msa0437150:CDS
GTACCAGCAGATGCTATCTG+TGG 0.690814 3_2:+91124763 None:intergenic
TATTGAATGGATGCTCAGAG+AGG 0.694968 3_2:+91123399 None:intergenic
ATGTGGATTTAAATCCTCAG+AGG 0.698508 3_2:+91125895 None:intergenic
ACACCTATAAAGGTTCCTTG+AGG 0.699526 3_2:+91125385 None:intergenic
GGTGTTTATGATGGTCATGG+TGG 0.701467 3_2:-91125367 Msa0437150:CDS
TGGAAATCAACTGTGGAGGG+TGG 0.718714 3_2:-91125568 Msa0437150:CDS
GGGCTGTGTTAGGAAGAGTG+AGG 0.729065 3_2:-91124678 Msa0437150:CDS
GAAGAAAGCAGAATTTAACA+GGG 0.732756 3_2:-91123928 Msa0437150:CDS
TGTAACAGGGAATAGCAAGG+AGG 0.777684 3_2:-91123641 Msa0437150:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTATTATTAAGTATGATTT+TGG - chr3_2:91124948-91124967 Msa0437150:intron 10.0%
!! TTAAAAAAAATAAGATTTCT+AGG + chr3_2:91125376-91125395 None:intergenic 10.0%
!!! ATTTTTTTTAAGTTTCTATA+TGG - chr3_2:91125385-91125404 Msa0437150:CDS 10.0%
!! AATAGTTAATAAGGATAATT+TGG + chr3_2:91123247-91123266 None:intergenic 15.0%
!! AAATTATCCTTATTAACTAT+TGG - chr3_2:91123246-91123265 Msa0437150:three_prime_UTR 15.0%
!!! TAGTTTTAAAAGTAATACTT+TGG - chr3_2:91123270-91123289 Msa0437150:three_prime_UTR 15.0%
!!! CATTTTGAAACAAATTTTTT+TGG - chr3_2:91123373-91123392 Msa0437150:three_prime_UTR 15.0%
!! TATAGGTATATATATAGATT+TGG - chr3_2:91123725-91123744 Msa0437150:intron 15.0%
!!! TATAGTCATAGATAATTTTT+TGG + chr3_2:91123826-91123845 None:intergenic 15.0%
!!! CTTTTATTACAAACATATAT+TGG - chr3_2:91124138-91124157 Msa0437150:intron 15.0%
!! AAATCAATATATAAGAATAC+CGG + chr3_2:91124511-91124530 None:intergenic 15.0%
!!! ATTTTATGAATTGATATTGA+TGG + chr3_2:91125580-91125599 None:intergenic 15.0%
!! AAACTAACCAATAGTTAATA+AGG + chr3_2:91123256-91123275 None:intergenic 20.0%
!! AGAAAAAGTTATTATTGCAT+TGG - chr3_2:91123333-91123352 Msa0437150:three_prime_UTR 20.0%
!!! ATTTTAGCTAAAAAACAAGT+TGG + chr3_2:91123678-91123697 None:intergenic 20.0%
!! ACAAACATATATTGGAAATA+TGG - chr3_2:91124146-91124165 Msa0437150:intron 20.0%
!!! TTTCTGAAATAGTTGTAAAT+TGG - chr3_2:91124449-91124468 Msa0437150:intron 20.0%
!! TTATATACCAATAATTACAG+CGG + chr3_2:91124484-91124503 None:intergenic 20.0%
!! ATTATTGGTATATAAAACAC+CGG - chr3_2:91124489-91124508 Msa0437150:intron 20.0%
!!! TTTTTTTTTGTGTGTAAATC+AGG - chr3_2:91124528-91124547 Msa0437150:CDS 20.0%
!! TATGATTGAAATTATAGAGT+AGG - chr3_2:91125013-91125032 Msa0437150:intron 20.0%
!!! AGATTTCTAGGTAATATTAA+CGG + chr3_2:91125364-91125383 None:intergenic 20.0%
! AATGATTAAGAGAAAGAGAA+TGG - chr3_2:91123213-91123232 Msa0437150:three_prime_UTR 25.0%
!!! CTAATCATGTCTATTTCTAT+AGG - chr3_2:91123708-91123727 Msa0437150:intron 25.0%
! TTGAAAATTCACCATAAAGA+TGG + chr3_2:91123935-91123954 None:intergenic 25.0%
!! TCTTTATGGTGAATTTTCAA+TGG - chr3_2:91123935-91123954 Msa0437150:CDS 25.0%
! AATTCAAGCAAATAGTTCAT+TGG - chr3_2:91123962-91123981 Msa0437150:intron 25.0%
!!! ATAAGTTACTTGGTTTTTCT+AGG + chr3_2:91124206-91124225 None:intergenic 25.0%
! ATAGTTGGATATAAGTTACT+TGG + chr3_2:91124216-91124235 None:intergenic 25.0%
!! CGTAGTTTTTGTCAAAATTA+CGG + chr3_2:91124288-91124307 None:intergenic 25.0%
! CAAATCTTTCAGTATATGTT+CGG + chr3_2:91124354-91124373 None:intergenic 25.0%
! TTTCAGAAAAGGTTATCAAA+AGG - chr3_2:91124574-91124593 Msa0437150:CDS 25.0%
! TTCAGAAAAGGTTATCAAAA+GGG - chr3_2:91124575-91124594 Msa0437150:CDS 25.0%
! CTATAATTTCAATCATACAG+TGG + chr3_2:91125010-91125029 None:intergenic 25.0%
! ACAAACTAATTGGTAAACTA+AGG - chr3_2:91125048-91125067 Msa0437150:intron 25.0%
!! CATATGTTTTTCTTATGCAA+CGG - chr3_2:91125145-91125164 Msa0437150:intron 25.0%
! AAGTTCTGTTAACACAAAAT+TGG + chr3_2:91125433-91125452 None:intergenic 25.0%
! TTCGTTTCTGTCAAAATTAA+AGG - chr3_2:91125706-91125725 Msa0437150:intron 25.0%
! CAATTACTGTAATATCATCA+TGG + chr3_2:91125897-91125916 None:intergenic 25.0%
!! TCTTGTTGTATAAAGAAGAT+AGG - chr3_2:91126058-91126077 Msa0437150:intron 25.0%
! CAACATAAATTTGTGATTGT+GGG + chr3_2:91126179-91126198 None:intergenic 25.0%
! TCAACATAAATTTGTGATTG+TGG + chr3_2:91126180-91126199 None:intergenic 25.0%
!! ATGTGCATTTAAAATTTGCT+TGG + chr3_2:91126227-91126246 None:intergenic 25.0%
! AGCTAATAAGCTAAAATATG+AGG + chr3_2:91126315-91126334 None:intergenic 25.0%
! AGTAGATAAGAGAAGGATTT+TGG + chr3_2:91123137-91123156 None:intergenic 30.0%
!! AAACGACACTCATTTTGAAA+CGG + chr3_2:91123164-91123183 None:intergenic 30.0%
!!! TTCAAAATGAGTGTCGTTTT+AGG - chr3_2:91123165-91123184 Msa0437150:three_prime_UTR 30.0%
! TGTGCAGAGTATTAATTAGA+GGG - chr3_2:91123299-91123318 Msa0437150:three_prime_UTR 30.0%
CTAAGTGTGTTCCTTTAATA+TGG - chr3_2:91123498-91123517 Msa0437150:CDS 30.0%
AAGATACAAACTAACGTTCA+AGG - chr3_2:91123550-91123569 Msa0437150:CDS 30.0%
AGAACAGAAAAAGTTACCTT+GGG + chr3_2:91123639-91123658 None:intergenic 30.0%
!! CTAAAAAACAAGTTGGTATG+GGG + chr3_2:91123671-91123690 None:intergenic 30.0%
!! GCTAAAAAACAAGTTGGTAT+GGG + chr3_2:91123672-91123691 None:intergenic 30.0%
!! AGCTAAAAAACAAGTTGGTA+TGG + chr3_2:91123673-91123692 None:intergenic 30.0%
TATCTATGACTATATCATGC+TGG - chr3_2:91123832-91123851 Msa0437150:CDS 30.0%
ATTGTTATGGTACAAGGATT+TGG - chr3_2:91123905-91123924 Msa0437150:CDS 30.0%
TTGTTATGGTACAAGGATTT+GGG - chr3_2:91123906-91123925 Msa0437150:CDS 30.0%
! ACCTATGAGTTCTGATTATT+TGG - chr3_2:91124010-91124029 Msa0437150:intron 30.0%
! CCTATGAGTTCTGATTATTT+GGG - chr3_2:91124011-91124030 Msa0437150:intron 30.0%
ACCTTTATAGGTGTTTATGA+TGG - chr3_2:91124044-91124063 Msa0437150:intron 30.0%
ACCATCATAAACACCTATAA+AGG + chr3_2:91124048-91124067 None:intergenic 30.0%
ACTCTTGTGATTATGGAAAA+AGG + chr3_2:91124263-91124282 None:intergenic 30.0%
AGTTTGATGTTATAACCTCA+AGG - chr3_2:91124410-91124429 Msa0437150:intron 30.0%
GTTTGATGTTATAACCTCAA+GGG - chr3_2:91124411-91124430 Msa0437150:intron 30.0%
GGAGATCATAACAAGTAAAT+TGG + chr3_2:91124989-91125008 None:intergenic 30.0%
ATTAGCTGCAACAAACTAAT+TGG - chr3_2:91125038-91125057 Msa0437150:intron 30.0%
GCATAAGAAAAACATATGCT+GGG + chr3_2:91125143-91125162 None:intergenic 30.0%
TGCATAAGAAAAACATATGC+TGG + chr3_2:91125144-91125163 None:intergenic 30.0%
AACCTTCAACCAAATGATTA+CGG - chr3_2:91125178-91125197 Msa0437150:intron 30.0%
GAATCGTAGTTAAATGTAGT+TGG - chr3_2:91125253-91125272 Msa0437150:intron 30.0%
CACAAAATTGGAGTAACATA+TGG + chr3_2:91125421-91125440 None:intergenic 30.0%
!!! TTAACAGAACTTGTTTTTGC+AGG - chr3_2:91125441-91125460 Msa0437150:CDS 30.0%
TGAAGAAAGCAGAATTTAAC+AGG - chr3_2:91125491-91125510 Msa0437150:CDS 30.0%
GAAGAAAGCAGAATTTAACA+GGG - chr3_2:91125492-91125511 Msa0437150:CDS 30.0%
AAGATGAGAAATTGATCTTC+AGG + chr3_2:91125607-91125626 None:intergenic 30.0%
ACAACTATGATTCATGTAAC+AGG - chr3_2:91125765-91125784 Msa0437150:intron 30.0%
CAACTATGATTCATGTAACA+GGG - chr3_2:91125766-91125785 Msa0437150:intron 30.0%
! GAGCTATTATCAATCAGTTT+TGG + chr3_2:91125934-91125953 None:intergenic 30.0%
! TGATTGATAATAGCTCTCTA+TGG - chr3_2:91125938-91125957 Msa0437150:five_prime_UTR 30.0%
AGGTTTCTTTCATTCTTTCT+AGG + chr3_2:91126004-91126023 None:intergenic 30.0%
!! TGCTAAAACCAAGTATTGAA+TGG + chr3_2:91126037-91126056 None:intergenic 30.0%
ACATTGTGATCTGATTATGA+AGG - chr3_2:91126243-91126262 Msa0437150:intron 30.0%
!!! ATTTTGGCTCTTTTTGACAG+AGG + chr3_2:91123121-91123140 None:intergenic 35.0%
! GTGTGCAGAGTATTAATTAG+AGG - chr3_2:91123298-91123317 Msa0437150:three_prime_UTR 35.0%
!! AAGCGACACTCATTTTGAAA+CGG - chr3_2:91123363-91123382 Msa0437150:three_prime_UTR 35.0%
AAGCACATATGGACTCAATA+TGG + chr3_2:91123472-91123491 None:intergenic 35.0%
! CTTAGCTGTAAAAGCACATA+TGG + chr3_2:91123483-91123502 None:intergenic 35.0%
ATGTGGATTTAAATCCTCAG+AGG + chr3_2:91123528-91123547 None:intergenic 35.0%
!! GTTAGTTTGTATCTTGCATG+TGG + chr3_2:91123545-91123564 None:intergenic 35.0%
CAGAACAGAAAAAGTTACCT+TGG + chr3_2:91123640-91123659 None:intergenic 35.0%
TGCTATTGTTCTTGTCTTCT+TGG + chr3_2:91123775-91123794 None:intergenic 35.0%
!! GGAAAGTTGATGGATTGTTA+TGG - chr3_2:91123892-91123911 Msa0437150:CDS 35.0%
!! TGATGGATTGTTATGGTACA+AGG - chr3_2:91123899-91123918 Msa0437150:CDS 35.0%
GATTTGGGAAACCATCTTTA+TGG - chr3_2:91123921-91123940 Msa0437150:CDS 35.0%
TCAAGCAAATAGTTCATTGG+AGG - chr3_2:91123965-91123984 Msa0437150:intron 35.0%
CCCAAATAATCAGAACTCAT+AGG + chr3_2:91124014-91124033 None:intergenic 35.0%
ATAGGTGTTTATGATGGTCA+TGG - chr3_2:91124050-91124069 Msa0437150:intron 35.0%
! TGAGAGCATACTCTTGAAAT+TGG + chr3_2:91124117-91124136 None:intergenic 35.0%
CCAAACATGCACTTAATAGT+TGG + chr3_2:91124231-91124250 None:intergenic 35.0%
CCAACTATTAAGTGCATGTT+TGG - chr3_2:91124228-91124247 Msa0437150:intron 35.0%
ATCAAACTATTGCCAACTCT+AGG + chr3_2:91124398-91124417 None:intergenic 35.0%
GTTAGTACCGCTGTAATTAT+TGG - chr3_2:91124474-91124493 Msa0437150:intron 35.0%
! GTTTTACAGCTGAAGATCAA+GGG - chr3_2:91124550-91124569 Msa0437150:CDS 35.0%
AGATCAAGGGATTTCAGAAA+AGG - chr3_2:91124563-91124582 Msa0437150:CDS 35.0%
ATGCTGTATATTGCAAATGC+AGG - chr3_2:91124711-91124730 Msa0437150:CDS 35.0%
AAACTGAGAGAGATGATGTT+CGG - chr3_2:91124823-91124842 Msa0437150:CDS 35.0%
ACCACAATCTGTGAATCATA+GGG + chr3_2:91124858-91124877 None:intergenic 35.0%
AACCACAATCTGTGAATCAT+AGG + chr3_2:91124859-91124878 None:intergenic 35.0%
TGGTTATGAAACACTCTGTT+TGG - chr3_2:91124874-91124893 Msa0437150:intron 35.0%
CATCATACAGGTACAAACTT+GGG - chr3_2:91124908-91124927 Msa0437150:intron 35.0%
AGAAAAACATATGCTGGGAA+AGG + chr3_2:91125138-91125157 None:intergenic 35.0%
! TTTTTCTTATGCAACGGTCA+AGG - chr3_2:91125151-91125170 Msa0437150:intron 35.0%
ACCTTCAACCAAATGATTAC+GGG - chr3_2:91125179-91125198 Msa0437150:intron 35.0%
!! AGTTGGTAGCCTTATAATTG+CGG - chr3_2:91125270-91125289 Msa0437150:intron 35.0%
! GAAAACTCTGATAGTGTTTC+AGG - chr3_2:91125311-91125330 Msa0437150:intron 35.0%
!! ATTGGCTTGAAAAAGGTTTC+AGG + chr3_2:91125544-91125563 None:intergenic 35.0%
!! ACTGAGAATTGGCTTGAAAA+AGG + chr3_2:91125551-91125570 None:intergenic 35.0%
TGAGAAATTGATCTTCAGGT+TGG + chr3_2:91125603-91125622 None:intergenic 35.0%
TTTCTCATCTTTGCTTCTGA+CGG - chr3_2:91125616-91125635 Msa0437150:five_prime_UTR 35.0%
TAACGATATTCACAGCTACT+TGG + chr3_2:91125663-91125682 None:intergenic 35.0%
! TCACTCCTTGTTCAATCTTT+TGG + chr3_2:91125864-91125883 None:intergenic 35.0%
AAAAGATTGAACAAGGAGTG+AGG - chr3_2:91125863-91125882 Msa0437150:five_prime_UTR 35.0%
CCATAACCATAAACTACTGT+GGG + chr3_2:91126108-91126127 None:intergenic 35.0%
ACCATAACCATAAACTACTG+TGG + chr3_2:91126109-91126128 None:intergenic 35.0%
TTGAAACTTGAAGAGTCTCA+TGG - chr3_2:91126133-91126152 Msa0437150:intron 35.0%
! CTTGTATTTTCTACCACCAT+TGG - chr3_2:91126289-91126308 Msa0437150:five_prime_UTR 35.0%
AAGCTAAAATATGAGGCCAA+TGG + chr3_2:91126308-91126327 None:intergenic 35.0%
TATTAGCTCAAACTCAAGCA+AGG - chr3_2:91126327-91126346 Msa0437150:five_prime_UTR 35.0%
ATTAGCTCAAACTCAAGCAA+GGG - chr3_2:91126328-91126347 Msa0437150:five_prime_UTR 35.0%
!!! GACACTCATTTTGAAACGGA+GGG + chr3_2:91123403-91123422 None:intergenic 40.0%
!!! GACACTCATTTTGAAACGGA+GGG - chr3_2:91123403-91123422 Msa0437150:three_prime_UTR 40.0%
! TCAGAGGTGTGCCATATTAA+AGG + chr3_2:91123512-91123531 None:intergenic 40.0%
TTTAATATGGCACACCTCTG+AGG - chr3_2:91123511-91123530 Msa0437150:CDS 40.0%
!! AAAAACAAGTTGGTATGGGG+TGG + chr3_2:91123668-91123687 None:intergenic 40.0%
ATCATGCTGGAAATCAACTG+TGG - chr3_2:91123845-91123864 Msa0437150:CDS 40.0%
GATGTGAAAGGGAAAGTTGA+TGG - chr3_2:91123882-91123901 Msa0437150:CDS 40.0%
TCATAGGTCCTGATTCAAGT+TGG + chr3_2:91123998-91124017 None:intergenic 40.0%
TCTGATTATTTGGGTCCTCA+AGG - chr3_2:91124020-91124039 Msa0437150:intron 40.0%
ACACCTATAAAGGTTCCTTG+AGG + chr3_2:91124038-91124057 None:intergenic 40.0%
! AGATTTGTTTCCTGTCCATG+AGG - chr3_2:91124367-91124386 Msa0437150:intron 40.0%
GATGTTATAACCTCAAGGGA+CGG - chr3_2:91124415-91124434 Msa0437150:intron 40.0%
ATGTTATAACCTCAAGGGAC+GGG - chr3_2:91124416-91124435 Msa0437150:intron 40.0%
! GGTTTTACAGCTGAAGATCA+AGG - chr3_2:91124549-91124568 Msa0437150:CDS 40.0%
! GCATTTTCTGCAACAGATGA+TGG - chr3_2:91124597-91124616 Msa0437150:CDS 40.0%
TGTCTCTTGTGAAGAAGCAA+TGG - chr3_2:91124625-91124644 Msa0437150:CDS 40.0%
ATCTGCTGGTACATGTTGTT+TGG - chr3_2:91124668-91124687 Msa0437150:CDS 40.0%
! TTGGCAGGGATAATTTGCAA+TGG - chr3_2:91124687-91124706 Msa0437150:CDS 40.0%
TCCCTATGATTCACAGATTG+TGG - chr3_2:91124854-91124873 Msa0437150:CDS 40.0%
GCATCATACAGGTACAAACT+TGG - chr3_2:91124907-91124926 Msa0437150:intron 40.0%
TCCCGTAATCATTTGGTTGA+AGG + chr3_2:91125183-91125202 None:intergenic 40.0%
ACGCGTCAAGGTTTAAATTG+TGG - chr3_2:91125202-91125221 Msa0437150:intron 40.0%
TTTCTGCGACCGCAATTATA+AGG + chr3_2:91125282-91125301 None:intergenic 40.0%
TTGCAGGTTTCAAGATCCAT+AGG - chr3_2:91125457-91125476 Msa0437150:CDS 40.0%
CTTCAGATATGCATCACCTA+TGG + chr3_2:91125476-91125495 None:intergenic 40.0%
TCAGGTAGTCTAAACTTCTG+TGG + chr3_2:91125526-91125545 None:intergenic 40.0%
TCTTTGCTTCTGACGGTTTA+TGG - chr3_2:91125623-91125642 Msa0437150:five_prime_UTR 40.0%
CTTTGCTTCTGACGGTTTAT+GGG - chr3_2:91125624-91125643 Msa0437150:five_prime_UTR 40.0%
TCATGTAACAGGGAATAGCA+AGG - chr3_2:91125776-91125795 Msa0437150:intron 40.0%
GACCTCCAAAAGATTGAACA+AGG - chr3_2:91125856-91125875 Msa0437150:five_prime_UTR 40.0%
! CTCCTTGTTCAATCTTTTGG+AGG + chr3_2:91125861-91125880 None:intergenic 40.0%
TCTCCTCTTTCAATCAGAGA+TGG - chr3_2:91125964-91125983 Msa0437150:five_prime_UTR 40.0%
TATTGAATGGATGCTCAGAG+AGG + chr3_2:91126024-91126043 None:intergenic 40.0%
CTGAGCATCCATTCAATACT+TGG - chr3_2:91126026-91126045 Msa0437150:intron 40.0%
CCCACAGTAGTTTATGGTTA+TGG - chr3_2:91126105-91126124 Msa0437150:intron 40.0%
CTAAAATATGAGGCCAATGG+TGG + chr3_2:91126305-91126324 None:intergenic 40.0%
!!! CGACACTCATTTTGAAACGG+AGG + chr3_2:91123402-91123421 None:intergenic 45.0%
!!! CGACACTCATTTTGAAACGG+AGG - chr3_2:91123402-91123421 Msa0437150:three_prime_UTR 45.0%
TGGACTCAATATGGCAGAGT+TGG + chr3_2:91123463-91123482 None:intergenic 45.0%
! CAGAGAAAACGAAAGCGTGT+TGG + chr3_2:91123585-91123604 None:intergenic 45.0%
! GCTACTAGTTTCTCATCCCA+AGG - chr3_2:91123620-91123639 Msa0437150:CDS 45.0%
ATGCTGGAAATCAACTGTGG+AGG - chr3_2:91123848-91123867 Msa0437150:CDS 45.0%
TGCTGGAAATCAACTGTGGA+GGG - chr3_2:91123849-91123868 Msa0437150:CDS 45.0%
!! GTGGTGATGGAGATGTGAAA+GGG - chr3_2:91123871-91123890 Msa0437150:CDS 45.0%
GATCGTAGCCAACTTGAATC+AGG - chr3_2:91123987-91124006 Msa0437150:intron 45.0%
GGTCCTCAAGGAACCTTTAT+AGG - chr3_2:91124032-91124051 Msa0437150:intron 45.0%
GGTGTTTATGATGGTCATGG+TGG - chr3_2:91124053-91124072 Msa0437150:intron 45.0%
TACGGCGACTCTTGTGATTA+TGG + chr3_2:91124270-91124289 None:intergenic 45.0%
AATTTGAATCCCGTCCCTTG+AGG + chr3_2:91124428-91124447 None:intergenic 45.0%
AAACCACAGATAGCATCTGC+TGG - chr3_2:91124654-91124673 Msa0437150:CDS 45.0%
! CTGGTACATGTTGTTTGGCA+GGG - chr3_2:91124673-91124692 Msa0437150:CDS 45.0%
TTGCAAATGCAGGAGACTCA+AGG - chr3_2:91124721-91124740 Msa0437150:CDS 45.0%
TGCAAATGCAGGAGACTCAA+GGG - chr3_2:91124722-91124741 Msa0437150:CDS 45.0%
TGTGTTAGGAAGAGTGAGGA+AGG - chr3_2:91124746-91124765 Msa0437150:CDS 45.0%
GTGTTAGGAAGAGTGAGGAA+GGG - chr3_2:91124747-91124766 Msa0437150:CDS 45.0%
AAGAGTGAGGAAGGGAACAA+GGG - chr3_2:91124755-91124774 Msa0437150:CDS 45.0%
AGTCTAAACTTCTGTGGCAG+AGG + chr3_2:91125520-91125539 None:intergenic 45.0%
GATGGCTCACTACTGAGAAT+TGG + chr3_2:91125562-91125581 None:intergenic 45.0%
GAAACGAAGCTTACATTGCG+TGG + chr3_2:91125694-91125713 None:intergenic 45.0%
TGTAACAGGGAATAGCAAGG+AGG - chr3_2:91125779-91125798 Msa0437150:intron 45.0%
CTGCCATCTCTGATTGAAAG+AGG + chr3_2:91125970-91125989 None:intergenic 45.0%
CTCTTTCAATCAGAGATGGC+AGG - chr3_2:91125968-91125987 Msa0437150:five_prime_UTR 45.0%
CAGTCTCCCACAGTAGTTTA+TGG - chr3_2:91126099-91126118 Msa0437150:intron 45.0%
CGGAGGGAGTAGATAAGAGA+AGG + chr3_2:91123144-91123163 None:intergenic 50.0%
TGGAAATCAACTGTGGAGGG+TGG - chr3_2:91123852-91123871 Msa0437150:CDS 50.0%
!! GGTGGTGATGGAGATGTGAA+AGG - chr3_2:91123870-91123889 Msa0437150:CDS 50.0%
CAACTCTAGGCAACACCTCA+TGG + chr3_2:91124385-91124404 None:intergenic 50.0%
!! CATGAGGTGTTGCCTAGAGT+TGG - chr3_2:91124383-91124402 Msa0437150:intron 50.0%
GTACCAGCAGATGCTATCTG+TGG + chr3_2:91124660-91124679 None:intergenic 50.0%
! GCTGGTACATGTTGTTTGGC+AGG - chr3_2:91124672-91124691 Msa0437150:CDS 50.0%
GAAGAGTGAGGAAGGGAACA+AGG - chr3_2:91124754-91124773 Msa0437150:CDS 50.0%
CACTCTGTTTGGCGTGTGAA+AGG - chr3_2:91124885-91124904 Msa0437150:intron 50.0%
!! GCGTGTGAAAGGCATCATAC+AGG - chr3_2:91124896-91124915 Msa0437150:intron 50.0%
TGACGCGTCCCGTAATCATT+TGG + chr3_2:91125190-91125209 None:intergenic 50.0%
AATGATTACGGGACGCGTCA+AGG - chr3_2:91125190-91125209 Msa0437150:intron 50.0%
TCAACTGTGGAGGGTGGTGA+TGG - chr3_2:91123858-91123877 Msa0437150:CDS 55.0%
CTAGGCAACACCTCATGGAC+AGG + chr3_2:91124380-91124399 None:intergenic 55.0%
GGAGACTCAAGGGCTGTGTT+AGG - chr3_2:91124732-91124751 Msa0437150:CDS 55.0%
GGGCTGTGTTAGGAAGAGTG+AGG - chr3_2:91124742-91124761 Msa0437150:CDS 55.0%
TAGTCTTCGCAGCTGCGTGC+AGG + chr3_2:91125078-91125097 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr3_2 gene 91123050 91126392 91123050 ID=Msa0437150;Name=Msa0437150
chr3_2 mRNA 91123050 91126392 91123050 ID=Msa0437150-mRNA-1;Parent=Msa0437150;Name=Msa0437150-mRNA-1;_AED=0.03;_eAED=0.03;_QI=429|1|1|1|0.6|0.5|6|387|370
chr3_2 exon 91123050 91123655 91123050 ID=Msa0437150-mRNA-1:exon:27234;Parent=Msa0437150-mRNA-1
chr3_2 exon 91123743 91123979 91123743 ID=Msa0437150-mRNA-1:exon:27233;Parent=Msa0437150-mRNA-1
chr3_2 exon 91124525 91124892 91124525 ID=Msa0437150-mRNA-1:exon:27232;Parent=Msa0437150-mRNA-1
chr3_2 exon 91125317 91125712 91125317 ID=Msa0437150-mRNA-1:exon:27231;Parent=Msa0437150-mRNA-1
chr3_2 exon 91125801 91126001 91125801 ID=Msa0437150-mRNA-1:exon:27230;Parent=Msa0437150-mRNA-1
chr3_2 exon 91126272 91126392 91126272 ID=Msa0437150-mRNA-1:exon:27229;Parent=Msa0437150-mRNA-1
chr3_2 five_prime_UTR 91126272 91126392 91126272 ID=Msa0437150-mRNA-1:five_prime_utr;Parent=Msa0437150-mRNA-1
chr3_2 five_prime_UTR 91125801 91126001 91125801 ID=Msa0437150-mRNA-1:five_prime_utr;Parent=Msa0437150-mRNA-1
chr3_2 five_prime_UTR 91125606 91125712 91125606 ID=Msa0437150-mRNA-1:five_prime_utr;Parent=Msa0437150-mRNA-1
chr3_2 CDS 91125317 91125605 91125317 ID=Msa0437150-mRNA-1:cds;Parent=Msa0437150-mRNA-1
chr3_2 CDS 91124525 91124892 91124525 ID=Msa0437150-mRNA-1:cds;Parent=Msa0437150-mRNA-1
chr3_2 CDS 91123743 91123979 91123743 ID=Msa0437150-mRNA-1:cds;Parent=Msa0437150-mRNA-1
chr3_2 CDS 91123437 91123655 91123437 ID=Msa0437150-mRNA-1:cds;Parent=Msa0437150-mRNA-1
chr3_2 three_prime_UTR 91123050 91123436 91123050 ID=Msa0437150-mRNA-1:three_prime_utr;Parent=Msa0437150-mRNA-1
Gene Sequence

>Msa0437150

ATGACTATATCATGCTGGAAATCAACTGTGGAGGGTGGTGATGGAGATGTGAAAGGGAAAGTTGATGGATTGTTATGGTACAAGGATTTGGGAAACCATCTTTATGGTGAATTTTCAATGGCTGTAATTCAAGCAAATAGTTCATTGGAGGATCGTAGCCAACTTGAATCAGGACCTATGAGTTCTGATTATTTGGGTCCTCAAGGAACCTTTATAGGTGTTTATGATGGTCATGGTGGAACTGCAGCTGCTCAGTTTGTTAATGACAATCTATTCTCCAATTTCAAGAGTTTTACAGCTGAAGATCAAGGGATTTCAGAAAAGGTTATCAAAAGGGCATTTTCTGCAACAGATGATGGTTTTCTGTCTCTTGTGAAGAAGCAATGGCAAAGCAAACCACAGATAGCATCTGCTGGTACATGTTGTTTGGCAGGGATAATTTGCAATGGTATGCTGTATATTGCAAATGCAGGAGACTCAAGGGCTGTGTTAGGAAGAGTGAGGAAGGGAACAAGGGAAACATTAGCTGTTCAGTTATCTACTGAACACAATGTTAATATAGAAACTGAGAGAGATGATGTTCGGTCAAAGCATCCCTATGATTCACAGATTGTGGTTATGAAACACTCTGTTTGGCGTGTGAAAGGCATCATACAGGTTTCAAGATCCATAGGTGATGCATATCTGAAGAAAGCAGAATTTAACAGGGAGCCTCTGCCACAGAAGTTTAGACTACCTGAAACCTTTTTCAAGCCAATTCTCAGTAGTGAGCCATCAATATCAATTCATAAAATCCAACCTGAAGATCAATTTCTCATCTTTGCTTCTGACGGTTTATGGGAGCATCTTAGCAACCAAGTAGCTGTGAATATCGTTAGCAACAATCCACGCAATGGAATAGCAAGGAGGCTTGTGAAAGCAGCACTTAAAGAAGCAGCGAAGAAGCGAGAAATAAGATTATCAGACCTCCAAAAGATTGAACAAGGAGTGAGGAGACACTTCCATGATGATATTACAGTAATTGTTGTATATCTAAATCCAAAACTGATTGATAATAGCTCTCTATGGAGTTCTCCTCTTTCAATCAGAGATGGCAGGCATGTCAATTCCTAG
Protein sequence

>Msa0437150

MTISCWKSTVEGGDGDVKGKVDGLLWYKDLGNHLYGEFSMAVIQANSSLEDRSQLESGPMSSDYLGPQGTFIGVYDGHGGTAAAQFVNDNLFSNFKSFTAEDQGISEKVIKRAFSATDDGFLSLVKKQWQSKPQIASAGTCCLAGIICNGMLYIANAGDSRAVLGRVRKGTRETLAVQLSTEHNVNIETERDDVRSKHPYDSQIVVMKHSVWRVKGIIQVSRSIGDAYLKKAEFNREPLPQKFRLPETFFKPILSSEPSISIHKIQPEDQFLIFASDGLWEHLSNQVAVNIVSNNPRNGIARRLVKAALKEAAKKREIRLSDLQKIEQGVRRHFHDDITVIVVYLNPKLIDNSSLWSSPLSIRDGRHVNS*