AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1448770

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1448770 MtrunA17_Chr7g0263281 98.108 370 7 0 1 370 1 370 0.0 753
Msa1448770 MtrunA17_Chr1g0181901 70.720 403 84 3 1 369 1 403 0.0 565
Msa1448770 MtrunA17_Chr1g0213351 66.954 348 111 2 5 348 13 360 4.01e-174 489
Msa1448770 MtrunA17_Chr7g0250061 64.754 366 125 2 4 365 6 371 1.03e-168 476
Msa1448770 MtrunA17_Chr1g0211251 63.115 366 131 2 4 365 11 376 1.12e-166 471
Msa1448770 MtrunA17_Chr8g0371321 62.931 348 128 1 17 363 25 372 1.45e-164 466
Msa1448770 MtrunA17_Chr2g0294291 56.438 365 145 3 5 363 12 368 1.29e-149 427
Msa1448770 MtrunA17_Chr1g0175381 54.268 328 141 3 22 349 38 356 2.78e-127 370
Msa1448770 MtrunA17_Chr1g0207571 54.079 331 142 2 18 346 16 338 1.26e-123 361
Msa1448770 MtrunA17_Chr3g0089711 46.154 325 158 6 29 346 33 347 1.07e-96 292
Msa1448770 MtrunA17_Chr3g0089721 46.154 325 158 6 29 346 33 347 2.20e-96 291
Msa1448770 MtrunA17_Chr7g0256751 46.462 325 157 6 29 346 34 348 5.96e-91 280
Msa1448770 MtrunA17_Chr7g0256771 45.221 272 138 4 29 298 35 297 1.77e-75 236
Msa1448770 MtrunA17_Chr3g0089691 45.318 267 126 5 86 346 9 261 1.76e-72 228
Msa1448770 MtrunA17_Chr4g0063111 35.952 331 193 6 22 348 34 349 1.17e-61 206
Msa1448770 MtrunA17_Chr4g0063051 33.618 351 188 12 3 348 1 311 8.61e-47 162
Msa1448770 MtrunA17_Chr2g0295991 31.962 316 169 7 71 345 166 476 2.75e-43 156
Msa1448770 MtrunA17_Chr7g0238501 29.530 298 181 6 71 349 263 550 2.07e-39 147
Msa1448770 MtrunA17_Chr4g0067301 29.483 329 173 7 71 345 164 487 1.05e-38 144
Msa1448770 MtrunA17_Chr4g0039061 30.435 299 176 7 70 349 201 486 2.20e-37 140
Msa1448770 MtrunA17_Chr7g0256761 41.117 197 93 5 159 350 7 185 4.71e-36 130
Msa1448770 MtrunA17_Chr4g0031521 30.466 279 152 6 108 349 426 699 1.83e-34 134
Msa1448770 MtrunA17_Chr1g0193691 29.236 301 148 5 109 349 592 887 2.09e-33 131
Msa1448770 MtrunA17_Chr4g0054811 29.450 309 159 6 102 356 475 778 1.39e-32 129
Msa1448770 MtrunA17_Chr3g0105371 30.686 277 136 7 70 345 117 338 5.70e-32 123
Msa1448770 MtrunA17_Chr5g0430481 34.529 223 104 6 70 291 153 334 1.10e-31 123
Msa1448770 MtrunA17_Chr3g0105351 29.964 277 138 7 70 345 93 314 1.77e-30 118
Msa1448770 MtrunA17_Chr1g0212961 28.710 310 148 9 47 353 106 345 1.32e-26 108
Msa1448770 MtrunA17_Chr1g0150451 30.627 271 138 9 47 313 88 312 4.07e-24 101
Msa1448770 MtrunA17_Chr4g0050381 30.877 285 144 12 35 314 30 266 4.90e-24 100
Msa1448770 MtrunA17_Chr2g0317021 30.576 278 128 10 71 345 117 332 5.49e-24 100
Msa1448770 MtrunA17_Chr3g0126511 30.870 230 112 6 66 291 120 306 7.36e-24 101
Msa1448770 MtrunA17_Chr5g0426141 31.034 319 144 13 31 343 31 279 7.72e-24 99.8
Msa1448770 MtrunA17_Chr3g0136711 29.000 300 144 11 47 343 45 278 1.66e-23 99.0
Msa1448770 MtrunA17_Chr5g0407161 29.371 286 129 9 71 346 124 346 1.89e-23 100
Msa1448770 MtrunA17_Chr4g0070871 29.297 256 122 7 54 291 231 445 9.13e-22 96.7
Msa1448770 MtrunA17_Chr2g0279151 29.078 282 137 9 64 343 58 278 1.54e-21 93.2
Msa1448770 MtrunA17_Chr4g0062951 32.065 184 115 6 105 279 1 183 1.95e-21 90.5
Msa1448770 MtrunA17_Chr3g0115851 28.797 316 155 13 31 343 31 279 1.21e-20 90.9
Msa1448770 MtrunA17_Chr8g0360351 27.187 320 164 12 30 346 29 282 1.33e-20 90.5
Msa1448770 MtrunA17_Chr2g0293811 29.825 285 141 9 70 346 99 332 2.31e-20 91.3
Msa1448770 MtrunA17_Chr1g0171571 29.537 281 140 10 72 347 419 646 4.17e-20 92.0
Msa1448770 MtrunA17_Chr3g0144111 28.223 287 116 9 69 347 25 229 4.39e-20 88.6
Msa1448770 MtrunA17_Chr3g0138281 28.421 285 143 11 66 345 117 345 2.32e-19 88.6
Msa1448770 MtrunA17_Chr4g0065141 28.777 278 142 9 70 343 123 348 3.17e-19 88.2
Msa1448770 MtrunA17_Chr3g0135961 28.197 305 160 12 60 345 275 539 3.74e-19 89.0
Msa1448770 MtrunA17_Chr1g0158781 27.726 321 176 11 35 349 113 383 1.27e-18 86.7
Msa1448770 MtrunA17_Chr5g0435721 27.586 232 129 5 68 292 220 419 8.33e-18 84.7
Msa1448770 MtrunA17_Chr3g0112291 25.000 296 158 10 71 345 123 375 9.24e-18 84.0
Msa1448770 MtrunA17_Chr4g0051661 28.926 242 125 10 71 308 183 381 1.60e-17 83.2
Msa1448770 MtrunA17_Chr1g0150781 27.419 310 149 12 67 348 285 546 2.03e-17 83.6
Msa1448770 MtrunA17_Chr7g0223641 30.493 223 110 7 135 345 7 196 3.00e-17 79.7
Msa1448770 MtrunA17_Chr1g0194191 26.573 286 149 11 70 346 117 350 1.28e-16 80.5
Msa1448770 MtrunA17_Chr3g0136811 25.614 285 138 10 52 292 33 287 2.13e-16 79.7
Msa1448770 MtrunA17_Chr1g0150071 26.592 267 122 10 70 292 51 287 8.89e-16 77.8
Msa1448770 MtrunA17_Chr2g0295401 28.873 284 148 11 71 326 92 349 1.34e-15 77.4
Msa1448770 MtrunA17_Chr7g0221691 32.075 159 90 5 152 309 245 386 8.28e-15 75.5
Msa1448770 MtrunA17_Chr4g0004971 26.154 325 160 14 63 351 69 349 1.18e-13 71.6
Msa1448770 MtrunA17_Chr8g0342431 31.928 166 83 5 152 310 210 352 2.47e-13 70.9
Msa1448770 MtrunA17_Chr3g0127091 26.482 253 138 10 70 297 88 317 9.87e-13 68.9
Msa1448770 MtrunA17_Chr5g0396731 24.399 291 165 7 71 349 107 354 1.00e-12 68.6
Msa1448770 MtrunA17_Chr1g0151781 27.170 265 136 10 56 292 53 288 3.03e-12 67.0
Msa1448770 MtrunA17_Chr1g0192421 27.465 284 152 13 71 326 89 346 6.77e-12 66.2
Msa1448770 MtrunA17_Chr4g0067711 30.000 200 104 8 138 326 174 348 7.50e-12 65.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1448770 AT3G12620.2 66.304 368 121 1 1 365 10 377 0.0 511
Msa1448770 AT3G12620.1 66.304 368 121 1 1 365 10 377 0.0 511
Msa1448770 AT3G55050.1 64.481 366 121 2 5 365 13 374 7.25e-177 497
Msa1448770 AT3G55050.2 64.481 366 121 2 5 365 13 374 7.25e-177 497
Msa1448770 AT3G51370.1 65.994 347 116 1 19 365 27 371 6.11e-174 489
Msa1448770 AT3G51370.3 65.994 347 116 1 19 365 27 371 6.11e-174 489
Msa1448770 AT5G66080.1 64.857 350 121 2 18 365 28 377 1.49e-168 476
Msa1448770 AT4G38520.2 62.178 349 131 1 16 363 26 374 1.48e-165 469
Msa1448770 AT4G38520.1 62.178 349 131 1 16 363 26 374 1.48e-165 469
Msa1448770 AT5G02760.1 62.264 371 136 3 1 370 1 368 8.96e-162 458
Msa1448770 AT3G17090.1 57.270 337 132 4 24 358 45 371 1.23e-135 392
Msa1448770 AT3G51370.2 64.130 276 97 1 90 365 13 286 2.30e-131 378
Msa1448770 AT5G66080.2 65.428 269 92 1 98 365 5 273 1.18e-127 368
Msa1448770 AT4G33920.1 51.862 349 149 3 22 359 20 360 1.51e-124 364
Msa1448770 AT5G06750.3 49.856 347 163 2 24 367 43 381 1.22e-119 352
Msa1448770 AT5G06750.1 49.856 347 163 2 24 367 43 381 1.22e-119 352
Msa1448770 AT3G17090.2 55.797 276 110 4 24 297 45 310 1.50e-106 316
Msa1448770 AT4G33920.2 53.480 273 116 2 98 359 5 277 1.65e-98 295
Msa1448770 AT5G06750.2 51.852 270 127 1 101 367 30 299 7.76e-94 283
Msa1448770 AT4G33920.3 54.656 247 101 2 124 359 1 247 3.65e-91 275
Msa1448770 AT3G16560.1 31.310 313 170 7 71 345 175 480 2.00e-42 154
Msa1448770 AT3G16560.4 31.310 313 170 7 71 345 175 480 2.00e-42 154
Msa1448770 AT3G16560.2 31.310 313 170 7 71 345 175 480 2.00e-42 154
Msa1448770 AT1G07160.1 34.520 281 129 7 67 345 149 376 7.19e-38 140
Msa1448770 AT5G02400.1 31.884 276 149 5 108 349 399 669 3.93e-37 142
Msa1448770 AT2G46920.3 28.713 303 150 5 108 349 542 839 2.35e-35 137
Msa1448770 AT2G46920.1 28.713 303 150 5 108 349 542 839 2.35e-35 137
Msa1448770 AT2G46920.2 28.713 303 150 5 108 349 542 839 2.35e-35 137
Msa1448770 AT2G46920.4 28.713 303 150 5 108 349 542 839 2.35e-35 137
Msa1448770 AT3G09400.1 30.037 273 151 6 108 349 382 645 8.67e-35 135
Msa1448770 AT1G07630.1 29.032 279 156 5 108 349 384 657 1.38e-34 135
Msa1448770 AT2G28890.1 29.391 279 155 5 108 349 376 649 1.88e-34 134
Msa1448770 AT2G40180.1 31.683 303 136 10 58 345 139 385 7.09e-34 129
Msa1448770 AT3G16560.3 30.742 283 151 7 71 315 175 450 2.33e-33 129
Msa1448770 AT2G35350.1 28.383 303 161 6 102 358 486 778 4.06e-33 131
Msa1448770 AT2G30020.1 32.975 279 129 9 70 345 169 392 4.02e-30 119
Msa1448770 AT1G67820.2 34.222 225 105 5 70 292 114 297 1.19e-28 115
Msa1448770 AT1G67820.1 34.222 225 105 5 70 292 150 333 1.63e-28 115
Msa1448770 AT2G33700.1 32.877 292 117 12 69 347 121 346 1.54e-25 106
Msa1448770 AT3G09400.2 28.870 239 130 6 108 315 382 611 1.57e-25 108
Msa1448770 AT4G31750.2 30.147 272 139 9 47 313 44 269 8.40e-25 103
Msa1448770 AT4G31750.1 30.147 272 139 9 47 313 44 269 1.02e-24 103
Msa1448770 AT4G31750.3 30.147 272 139 9 47 313 44 269 1.02e-24 103
Msa1448770 AT3G51470.2 28.763 299 137 11 62 348 52 286 3.04e-24 102
Msa1448770 AT3G51470.1 28.763 299 137 11 62 348 96 330 6.10e-24 101
Msa1448770 AT1G43900.1 32.526 289 121 11 62 343 145 366 1.12e-23 101
Msa1448770 AT5G10740.1 30.556 288 146 11 31 313 30 268 1.42e-23 100
Msa1448770 AT5G10740.2 30.556 288 146 11 31 313 30 268 1.42e-23 100
Msa1448770 AT1G78200.1 29.393 313 152 12 35 343 33 280 7.25e-23 97.4
Msa1448770 AT1G78200.3 29.393 313 152 12 35 343 33 280 7.25e-23 97.4
Msa1448770 AT1G78200.2 29.393 313 152 12 35 343 33 280 7.25e-23 97.4
Msa1448770 AT5G53140.1 26.756 299 146 9 71 359 132 367 4.07e-22 97.4
Msa1448770 AT5G53140.2 26.756 299 146 9 71 359 132 367 4.07e-22 97.4
Msa1448770 AT1G78200.5 29.021 286 139 10 62 343 10 235 5.66e-22 94.0
Msa1448770 AT1G78200.4 29.021 286 139 10 62 343 10 235 5.66e-22 94.0
Msa1448770 AT2G25620.1 28.165 316 165 13 43 348 96 359 7.41e-22 96.3
Msa1448770 AT2G25620.2 28.165 316 165 13 43 348 96 359 7.41e-22 96.3
Msa1448770 AT4G28400.3 30.315 254 129 9 66 314 40 250 1.84e-21 92.8
Msa1448770 AT5G24940.1 29.825 285 146 11 31 310 30 265 2.98e-21 95.1
Msa1448770 AT4G28400.1 29.371 286 133 10 66 346 62 283 3.36e-21 92.8
Msa1448770 AT2G20630.1 29.242 277 129 10 71 343 63 276 8.70e-21 91.7
Msa1448770 AT2G20630.2 28.986 276 129 10 71 342 63 275 4.81e-20 89.7
Msa1448770 AT1G07430.1 28.696 230 124 5 71 292 157 354 7.36e-20 90.9
Msa1448770 AT1G34750.2 28.526 312 152 12 38 345 37 281 2.23e-19 87.4
Msa1448770 AT1G34750.1 28.526 312 152 12 38 345 37 281 2.23e-19 87.4
Msa1448770 AT1G34750.3 28.526 312 152 12 38 345 37 281 2.23e-19 87.4
Msa1448770 AT2G29380.1 28.571 301 162 10 57 345 101 360 2.61e-19 88.6
Msa1448770 AT1G48040.1 30.088 339 156 14 39 359 81 356 2.65e-19 89.0
Msa1448770 AT3G17250.1 28.571 273 140 10 62 314 142 379 3.84e-19 88.6
Msa1448770 AT1G22280.3 28.349 321 160 13 29 343 28 284 5.20e-19 86.7
Msa1448770 AT4G08260.1 27.698 278 112 10 70 345 18 208 6.65e-19 84.7
Msa1448770 AT4G26080.1 26.087 322 184 10 54 361 153 434 1.66e-18 87.0
Msa1448770 AT1G22280.1 28.481 316 160 12 29 343 28 278 1.92e-18 84.7
Msa1448770 AT3G62260.3 30.242 248 134 9 71 309 27 244 2.00e-18 85.1
Msa1448770 AT3G15260.2 29.603 277 128 11 71 343 73 286 2.94e-18 84.3
Msa1448770 AT3G15260.1 29.603 277 128 11 71 343 73 286 2.94e-18 84.3
Msa1448770 AT2G40860.1 28.470 281 144 8 71 347 423 650 3.27e-18 86.7
Msa1448770 AT2G40860.4 28.470 281 144 8 71 347 325 552 3.62e-18 86.3
Msa1448770 AT5G51760.2 27.083 240 124 8 71 291 114 321 4.82e-18 85.1
Msa1448770 AT5G51760.1 27.083 240 124 8 71 291 144 351 6.12e-18 85.1
Msa1448770 AT3G62260.1 30.242 248 134 9 71 309 116 333 7.20e-18 84.7
Msa1448770 AT3G62260.2 30.242 248 134 9 71 309 117 334 7.26e-18 84.7
Msa1448770 AT2G34740.1 28.938 273 148 10 44 314 96 324 1.11e-17 83.6
Msa1448770 AT2G25070.1 25.887 282 141 8 52 292 33 287 2.20e-17 82.8
Msa1448770 AT2G25070.2 25.887 282 141 8 52 292 33 287 2.20e-17 82.8
Msa1448770 AT3G16800.2 28.428 299 153 12 70 347 92 350 2.34e-17 82.8
Msa1448770 AT3G16800.1 28.428 299 153 12 70 347 92 350 2.34e-17 82.8
Msa1448770 AT3G16800.3 28.428 299 153 12 70 347 92 350 2.34e-17 82.8
Msa1448770 AT5G59220.1 28.384 229 123 6 71 292 148 342 6.42e-17 82.0
Msa1448770 AT3G16800.4 29.832 238 117 9 122 347 56 255 8.71e-17 79.7
Msa1448770 AT3G16800.6 29.832 238 117 9 122 347 56 255 8.71e-17 79.7
Msa1448770 AT2G40860.2 28.226 248 137 7 71 311 423 636 2.12e-16 81.3
Msa1448770 AT1G72770.4 27.308 260 121 5 60 291 227 446 2.46e-16 80.9
Msa1448770 AT1G72770.1 27.308 260 121 5 60 291 227 446 2.46e-16 80.9
Msa1448770 AT1G72770.3 27.308 260 121 5 60 291 227 446 2.46e-16 80.9
Msa1448770 AT2G34740.2 29.289 239 124 9 44 281 110 304 4.30e-16 79.0
Msa1448770 AT1G17550.1 26.603 312 151 12 71 348 237 504 4.41e-16 80.1
Msa1448770 AT1G17550.2 26.603 312 151 12 71 348 237 504 4.41e-16 80.1
Msa1448770 AT2G34740.3 29.289 239 124 9 44 281 92 286 4.41e-16 78.6
Msa1448770 AT1G18030.1 28.736 261 129 9 72 311 110 334 2.08e-15 77.0
Msa1448770 AT5G27930.1 28.000 300 152 14 71 344 92 353 4.85e-15 76.3
Msa1448770 AT5G27930.2 28.000 300 152 14 71 344 92 353 4.85e-15 76.3
Msa1448770 AT3G11410.1 24.314 255 134 7 71 308 137 349 5.42e-15 76.3
Msa1448770 AT1G34750.4 27.823 248 128 9 38 281 37 237 5.08e-14 71.6
Msa1448770 AT3G05640.2 27.957 279 152 13 71 326 93 345 5.14e-14 72.8
Msa1448770 AT3G05640.1 27.957 279 152 13 71 326 93 345 5.14e-14 72.8
Msa1448770 AT3G05640.3 27.957 279 152 13 71 326 93 345 5.14e-14 72.8
Msa1448770 AT5G26010.1 26.629 353 200 13 11 348 19 327 6.31e-14 72.4
Msa1448770 AT4G32950.1 26.014 296 167 12 64 348 65 319 1.44e-12 68.6

Find 116 sgRNAs with CRISPR-Local

Find 193 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACCTATGAGTTCTGATTATT+TGG 0.094977 tig0032326:+21349 Msa1448770:CDS
TGCTATTGTTCTTGTCTTCT+TGG 0.118706 tig0032326:-21111 None:intergenic
TCACTCCTTGTTCAATCTTT+TGG 0.144007 tig0032326:-23211 None:intergenic
TCTTTGCTTCTGACGGTTTA+TGG 0.156191 tig0032326:+22973 Msa1448770:CDS
CAGTCTCCCACAGTAGTTTA+TGG 0.181885 tig0032326:+23449 Msa1448770:three_prime_UTR
AATAGATAAGAGAAGGATTT+TGG 0.185679 tig0032326:-20748 None:intergenic
GATTTGGGAAACCATCTTTA+TGG 0.196034 tig0032326:+21260 Msa1448770:CDS
GGTCCTCAAGGAACCTTTAT+AGG 0.223798 tig0032326:+21371 Msa1448770:CDS
CCCACAGTAGTTTATGGTTA+TGG 0.252793 tig0032326:+23455 Msa1448770:three_prime_UTR
TGAGAGCATACTCTTGAAAT+TGG 0.253266 tig0032326:-21453 None:intergenic
AGGTTTCTTTCATTCTTTCT+AGG 0.264590 tig0032326:-23351 None:intergenic
ACTGAGAATTGGCTTGAAAA+AGG 0.264897 tig0032326:-22898 None:intergenic
CTAAGTGTGTTCCTTTAATA+TGG 0.271025 tig0032326:+20837 Msa1448770:five_prime_UTR
CTTTGCTTCTGACGGTTTAT+GGG 0.274010 tig0032326:+22974 Msa1448770:CDS
GAGCTATTATCAATCAGTTT+TGG 0.288253 tig0032326:-23281 None:intergenic
ATTGTTATGGTACAAGGATT+TGG 0.289189 tig0032326:+21244 Msa1448770:CDS
TCAGAGGTGTGCCATATTAA+AGG 0.291940 tig0032326:-20848 None:intergenic
TGAAGAAAGCAGAATTTAAC+AGG 0.297447 tig0032326:+22841 Msa1448770:CDS
CTGAGCATCCATTCAATACT+TGG 0.302446 tig0032326:+23376 Msa1448770:three_prime_UTR
TTGTTATGGTACAAGGATTT+GGG 0.311993 tig0032326:+21245 Msa1448770:CDS
AAGATGAGAAATTGATCTTC+AGG 0.326898 tig0032326:-22954 None:intergenic
TATAGGTATATATATAGATT+TGG 0.334918 tig0032326:+21064 Msa1448770:intron
ACCATCATAAACACCTATAA+AGG 0.367844 tig0032326:-21384 None:intergenic
AACCACAATCTGTGAATCAT+AGG 0.369310 tig0032326:-22195 None:intergenic
CTTAGCTGTAAAAGCACATA+TGG 0.371280 tig0032326:-20819 None:intergenic
CCTATGAGTTCTGATTATTT+GGG 0.385684 tig0032326:+21350 Msa1448770:CDS
CAACATAAATTTGTGATTGT+GGG 0.387941 tig0032326:-23526 None:intergenic
GATGGCTCACTACTGAGAAT+TGG 0.390286 tig0032326:-22909 None:intergenic
ATCTGCTGGTACATGTTGTT+TGG 0.408579 tig0032326:+22007 Msa1448770:CDS
TTGAAAATTCACCATAAAGA+TGG 0.408650 tig0032326:-21271 None:intergenic
AATTCAAGCAAATAGTTCAT+TGG 0.412793 tig0032326:+21301 Msa1448770:CDS
TCTTGTTGTATAAAGAAGAT+AGG 0.421011 tig0032326:+23408 Msa1448770:three_prime_UTR
GAAGAGTGAGGAAGGGAACA+AGG 0.421093 tig0032326:+22093 Msa1448770:CDS
TTTCAGAAAAGGTTATCAAA+AGG 0.431288 tig0032326:+21913 Msa1448770:CDS
TGATTGATAATAGCTCTCTA+TGG 0.431364 tig0032326:+23288 Msa1448770:CDS
GGAGACTCAAGGGCTGTGTT+AGG 0.432476 tig0032326:+22071 Msa1448770:CDS
GGAAAGTTGATGGATTGTTA+TGG 0.432672 tig0032326:+21231 Msa1448770:CDS
GATCGTAGCCAACTTGAATC+AGG 0.435753 tig0032326:+21326 Msa1448770:CDS
AAGCACATATGGACTCAATA+TGG 0.440468 tig0032326:-20808 None:intergenic
TAACGATATTCACAGCTACT+TGG 0.443990 tig0032326:-23010 None:intergenic
CTTCAGATATGCATCACCTA+TGG 0.450543 tig0032326:-22823 None:intergenic
TCAACATAAATTTGTGATTG+TGG 0.453642 tig0032326:-23527 None:intergenic
TGATGGATTGTTATGGTACA+AGG 0.456025 tig0032326:+21238 Msa1448770:CDS
GCTGGTACATGTTGTTTGGC+AGG 0.456613 tig0032326:+22011 Msa1448770:CDS
TTCAGAAAAGGTTATCAAAA+GGG 0.466769 tig0032326:+21914 Msa1448770:CDS
AGATCAAGGGATTTCAGAAA+AGG 0.471446 tig0032326:+21902 Msa1448770:CDS
TTTCTCATCTTTGCTTCTGA+CGG 0.472510 tig0032326:+22966 Msa1448770:CDS
CACTCTGTTTGGCGTGTGAA+AGG 0.474473 tig0032326:+22224 Msa1448770:CDS
CCCAAATAATCAGAACTCAT+AGG 0.475948 tig0032326:-21350 None:intergenic
TGTGTTAGGAAGAGTGAGGA+AGG 0.477652 tig0032326:+22085 Msa1448770:CDS
AGCTAATAAGCTAAAATATG+AGG 0.478377 tig0032326:-23662 None:intergenic
TGCTAAAACCAAGTATTGAA+TGG 0.481415 tig0032326:-23384 None:intergenic
AAGATACAAACTAACGTTCA+AGG 0.484502 tig0032326:+20889 Msa1448770:five_prime_UTR
TGGTTATGAAACACTCTGTT+TGG 0.490832 tig0032326:+22213 Msa1448770:CDS
AAGAGTGAGGAAGGGAACAA+GGG 0.493346 tig0032326:+22094 Msa1448770:CDS
TGAGAAATTGATCTTCAGGT+TGG 0.496104 tig0032326:-22950 None:intergenic
ATGTGCATTTAAAATTTGCT+TGG 0.501597 tig0032326:-23574 None:intergenic
GATGTGAAAGGGAAAGTTGA+TGG 0.502197 tig0032326:+21221 Msa1448770:CDS
ACCTTTATAGGTGTTTATGA+TGG 0.502488 tig0032326:+21383 Msa1448770:CDS
TTGCAGGTTTCAAGATCCAT+AGG 0.507189 tig0032326:+22807 Msa1448770:intron
GGTGGTGATGGAGATGTGAA+AGG 0.507401 tig0032326:+21209 Msa1448770:CDS
TGGACTCAATATGGCAGAGT+TGG 0.517378 tig0032326:-20799 None:intergenic
CTGCCATCTCTGATTGAAAG+AGG 0.518398 tig0032326:-23317 None:intergenic
GAAACGAAGCTTACATTGCG+TGG 0.519390 tig0032326:-23041 None:intergenic
CTCTTTCAATCAGAGATGGC+AGG 0.520547 tig0032326:+23318 Msa1448770:CDS
TCAACTGTGGAGGGTGGTGA+TGG 0.527360 tig0032326:+21197 Msa1448770:CDS
TCCCTATGATTCACAGATTG+TGG 0.527683 tig0032326:+22193 Msa1448770:CDS
ATAGGTGTTTATGATGGTCA+TGG 0.527745 tig0032326:+21389 Msa1448770:CDS
AAACTGAGAGAGATGATGTT+CGG 0.531123 tig0032326:+22162 Msa1448770:CDS
CTGGTACATGTTGTTTGGCA+GGG 0.535327 tig0032326:+22012 Msa1448770:CDS
ATGAAACAATAGATAAGAGA+AGG 0.535359 tig0032326:-20755 None:intergenic
TCAAGCAAATAGTTCATTGG+AGG 0.537174 tig0032326:+21304 Msa1448770:CDS
TATCTATGACTATATCATGC+TGG 0.539611 tig0032326:+21171 Msa1448770:exon
TTGAAACTTGAAGAGTCTCA+TGG 0.548045 tig0032326:+23483 Msa1448770:three_prime_UTR
TCTGATTATTTGGGTCCTCA+AGG 0.548992 tig0032326:+21359 Msa1448770:CDS
GCGTGTGAAAGGCATCATAC+AGG 0.551663 tig0032326:+22235 Msa1448770:CDS
GTTAGTTTGTATCTTGCATG+TGG 0.552758 tig0032326:-20881 None:intergenic
TGTCTCTTGTGAAGAAGCAA+TGG 0.558482 tig0032326:+21964 Msa1448770:CDS
AGTCTAAACTTCTGTGGCAG+AGG 0.558697 tig0032326:-22867 None:intergenic
GTGGTGATGGAGATGTGAAA+GGG 0.558872 tig0032326:+21210 Msa1448770:CDS
TTGCAAATGCAGGAGACTCA+AGG 0.563487 tig0032326:+22060 Msa1448770:CDS
TCTCCTCTTTCAATCAGAGA+TGG 0.566031 tig0032326:+23314 Msa1448770:CDS
TCATAGGTCCTGATTCAAGT+TGG 0.579655 tig0032326:-21334 None:intergenic
CAGAGAAAACGAAAGCGTGT+TGG 0.579973 tig0032326:-20921 None:intergenic
CAATTACTGTAATATCATCA+TGG 0.580958 tig0032326:-23244 None:intergenic
ACCACAATCTGTGAATCATA+GGG 0.581742 tig0032326:-22194 None:intergenic
TGCAAATGCAGGAGACTCAA+GGG 0.586962 tig0032326:+22061 Msa1448770:CDS
TATTAGCTCAAACTCAAGCA+AGG 0.587242 tig0032326:+23677 Msa1448770:three_prime_UTR
CCATAACCATAAACTACTGT+GGG 0.588499 tig0032326:-23455 None:intergenic
TGCTGGAAATCAACTGTGGA+GGG 0.596448 tig0032326:+21188 Msa1448770:CDS
GCTACTAGTTTCTCATCCCA+AGG 0.598922 tig0032326:+20959 Msa1448770:five_prime_UTR
TCAGGTAGTCTAAACTTCTG+TGG 0.603011 tig0032326:-22873 None:intergenic
ACATTGTGATCTGATTATGA+AGG 0.605518 tig0032326:+23593 Msa1448770:three_prime_UTR
TTTAATATGGCACACCTCTG+AGG 0.608672 tig0032326:+20850 Msa1448770:five_prime_UTR
CTAAAATATGAGGCCAATGG+TGG 0.609733 tig0032326:-23652 None:intergenic
ATGCTGTATATTGCAAATGC+AGG 0.610667 tig0032326:+22050 Msa1448770:CDS
AAGCTAAAATATGAGGCCAA+TGG 0.612731 tig0032326:-23655 None:intergenic
AAACCACAGATAGCATCTGC+TGG 0.614223 tig0032326:+21993 Msa1448770:CDS
ACCATAACCATAAACTACTG+TGG 0.618055 tig0032326:-23456 None:intergenic
GACCTCCAAAAGATTGAACA+AGG 0.625989 tig0032326:+23206 Msa1448770:CDS
TTGGCAGGGATAATTTGCAA+TGG 0.628153 tig0032326:+22026 Msa1448770:CDS
AAAAGATTGAACAAGGAGTG+AGG 0.629700 tig0032326:+23213 Msa1448770:CDS
ATTAGCTCAAACTCAAGCAA+GGG 0.643945 tig0032326:+23678 Msa1448770:three_prime_UTR
ATGCTGGAAATCAACTGTGG+AGG 0.664898 tig0032326:+21187 Msa1448770:CDS
ATCATGCTGGAAATCAACTG+TGG 0.668033 tig0032326:+21184 Msa1448770:CDS
TCATGTAACAGGGAATAGCA+AGG 0.668087 tig0032326:+23126 Msa1448770:intron
GTGTTAGGAAGAGTGAGGAA+GGG 0.687678 tig0032326:+22086 Msa1448770:CDS
GTACCAGCAGATGCTATCTG+TGG 0.690814 tig0032326:-21996 None:intergenic
TATTGAATGGATGCTCAGAG+AGG 0.694968 tig0032326:-23371 None:intergenic
ATGTGGATTTAAATCCTCAG+AGG 0.698508 tig0032326:-20864 None:intergenic
ACACCTATAAAGGTTCCTTG+AGG 0.699526 tig0032326:-21374 None:intergenic
GGTGTTTATGATGGTCATGG+TGG 0.701467 tig0032326:+21392 Msa1448770:CDS
TGGAAATCAACTGTGGAGGG+TGG 0.718714 tig0032326:+21191 Msa1448770:CDS
GGGCTGTGTTAGGAAGAGTG+AGG 0.729065 tig0032326:+22081 Msa1448770:CDS
GAAGAAAGCAGAATTTAACA+GGG 0.732756 tig0032326:+22842 Msa1448770:CDS
TGTAACAGGGAATAGCAAGG+AGG 0.777684 tig0032326:+23129 Msa1448770:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAAAAAAAATAAGATTTCT+AGG - tig0032326:22726-22745 None:intergenic 10.0%
!!! ATTTTTTTTAAGTTTCTATA+TGG + tig0032326:22735-22754 Msa1448770:intron 10.0%
!! TATAGGTATATATATAGATT+TGG + tig0032326:21064-21083 Msa1448770:intron 15.0%
!!! TATAGTCATAGATAATTTTT+TGG - tig0032326:21165-21184 None:intergenic 15.0%
!!! CTTTTATTACAAACATATAT+TGG + tig0032326:21477-21496 Msa1448770:intron 15.0%
!! AAATCAATATATAAGAATAC+CGG - tig0032326:21850-21869 None:intergenic 15.0%
!!! TTTATTACTAAGTATGATTT+TGG + tig0032326:22288-22307 Msa1448770:intron 15.0%
!!! ATTTTATGAATTGATATTGA+TGG - tig0032326:22930-22949 None:intergenic 15.0%
!!! ATTTTAGCTAAAAAACAAGT+TGG - tig0032326:21017-21036 None:intergenic 20.0%
!! ACAAACATATATTGGAAATA+TGG + tig0032326:21485-21504 Msa1448770:intron 20.0%
!!! TTTCTGAAATAGTTGTAAAT+TGG + tig0032326:21788-21807 Msa1448770:intron 20.0%
!! TTATATACCAATAATTACAG+CGG - tig0032326:21823-21842 None:intergenic 20.0%
!! ATTATTGGTATATAAAACAC+CGG + tig0032326:21828-21847 Msa1448770:intron 20.0%
!!! TTTTTTTTTGTGTGTAAATC+AGG + tig0032326:21867-21886 Msa1448770:intron 20.0%
!! ACAAATCATAACAAGTTAAT+TGG - tig0032326:22329-22348 None:intergenic 20.0%
!! TATGATTGAAATTATAGAGT+AGG + tig0032326:22363-22382 Msa1448770:intron 20.0%
!!! AGATTTCTAGGTAATATTAA+CGG - tig0032326:22714-22733 None:intergenic 20.0%
!! AATAGATAAGAGAAGGATTT+TGG - tig0032326:20751-20770 None:intergenic 25.0%
! ATGAAACAATAGATAAGAGA+AGG - tig0032326:20758-20777 None:intergenic 25.0%
!!! CTAATCATGTCTATTTCTAT+AGG + tig0032326:21047-21066 Msa1448770:intron 25.0%
! TTGAAAATTCACCATAAAGA+TGG - tig0032326:21274-21293 None:intergenic 25.0%
!! TCTTTATGGTGAATTTTCAA+TGG + tig0032326:21274-21293 Msa1448770:CDS 25.0%
! AATTCAAGCAAATAGTTCAT+TGG + tig0032326:21301-21320 Msa1448770:CDS 25.0%
!!! ATAAGTTACTTGGTTTTTCT+AGG - tig0032326:21545-21564 None:intergenic 25.0%
! ATAGTTGGATATAAGTTACT+TGG - tig0032326:21555-21574 None:intergenic 25.0%
!! CGTAGTTTTTGTCAAAATTA+CGG - tig0032326:21627-21646 None:intergenic 25.0%
! CAAATCTTTCAGTATATGTT+CGG - tig0032326:21693-21712 None:intergenic 25.0%
! TTTCAGAAAAGGTTATCAAA+AGG + tig0032326:21913-21932 Msa1448770:CDS 25.0%
! TTCAGAAAAGGTTATCAAAA+GGG + tig0032326:21914-21933 Msa1448770:CDS 25.0%
! CTATAATTTCAATCATACAG+TGG - tig0032326:22360-22379 None:intergenic 25.0%
! ACAAACTAATTGGTAAACTA+AGG + tig0032326:22398-22417 Msa1448770:intron 25.0%
!! CATATGTTTTTCTTATGCAA+CGG + tig0032326:22495-22514 Msa1448770:intron 25.0%
! AAGTTCTGTTAACACAAAAT+TGG - tig0032326:22783-22802 None:intergenic 25.0%
! TTCGTTTCTGTCAAAATTAA+AGG + tig0032326:23056-23075 Msa1448770:intron 25.0%
! CAATTACTGTAATATCATCA+TGG - tig0032326:23247-23266 None:intergenic 25.0%
!! TCTTGTTGTATAAAGAAGAT+AGG + tig0032326:23408-23427 Msa1448770:three_prime_UTR 25.0%
! CAACATAAATTTGTGATTGT+GGG - tig0032326:23529-23548 None:intergenic 25.0%
! TCAACATAAATTTGTGATTG+TGG - tig0032326:23530-23549 None:intergenic 25.0%
!! ATGTGCATTTAAAATTTGCT+TGG - tig0032326:23577-23596 None:intergenic 25.0%
! AGCTAATAAGCTAAAATATG+AGG - tig0032326:23665-23684 None:intergenic 25.0%
CTAAGTGTGTTCCTTTAATA+TGG + tig0032326:20837-20856 Msa1448770:five_prime_UTR 30.0%
AAGATACAAACTAACGTTCA+AGG + tig0032326:20889-20908 Msa1448770:five_prime_UTR 30.0%
AGAACAGAAAAAGTTACCTT+GGG - tig0032326:20978-20997 None:intergenic 30.0%
!! CTAAAAAACAAGTTGGTATG+GGG - tig0032326:21010-21029 None:intergenic 30.0%
!! GCTAAAAAACAAGTTGGTAT+GGG - tig0032326:21011-21030 None:intergenic 30.0%
!! AGCTAAAAAACAAGTTGGTA+TGG - tig0032326:21012-21031 None:intergenic 30.0%
TATCTATGACTATATCATGC+TGG + tig0032326:21171-21190 Msa1448770:exon 30.0%
ATTGTTATGGTACAAGGATT+TGG + tig0032326:21244-21263 Msa1448770:CDS 30.0%
TTGTTATGGTACAAGGATTT+GGG + tig0032326:21245-21264 Msa1448770:CDS 30.0%
! ACCTATGAGTTCTGATTATT+TGG + tig0032326:21349-21368 Msa1448770:CDS 30.0%
! CCTATGAGTTCTGATTATTT+GGG + tig0032326:21350-21369 Msa1448770:CDS 30.0%
ACCTTTATAGGTGTTTATGA+TGG + tig0032326:21383-21402 Msa1448770:CDS 30.0%
ACCATCATAAACACCTATAA+AGG - tig0032326:21387-21406 None:intergenic 30.0%
ACTCTTGTGATTATGGAAAA+AGG - tig0032326:21602-21621 None:intergenic 30.0%
AGTTTGATGTTATAACCTCA+AGG + tig0032326:21749-21768 Msa1448770:intron 30.0%
GTTTGATGTTATAACCTCAA+GGG + tig0032326:21750-21769 Msa1448770:intron 30.0%
ATTAGCTGCAACAAACTAAT+TGG + tig0032326:22388-22407 Msa1448770:intron 30.0%
GCATAAGAAAAACATATGCT+GGG - tig0032326:22493-22512 None:intergenic 30.0%
TGCATAAGAAAAACATATGC+TGG - tig0032326:22494-22513 None:intergenic 30.0%
AACCTTCAACCAAATGATTA+CGG + tig0032326:22528-22547 Msa1448770:intron 30.0%
GAATCGTAGTTAAATGTAGT+TGG + tig0032326:22603-22622 Msa1448770:intron 30.0%
CACAAAATTGGAGTAACATA+TGG - tig0032326:22771-22790 None:intergenic 30.0%
!!! TTAACAGAACTTGTTTTTGC+AGG + tig0032326:22791-22810 Msa1448770:intron 30.0%
TGAAGAAAGCAGAATTTAAC+AGG + tig0032326:22841-22860 Msa1448770:CDS 30.0%
GAAGAAAGCAGAATTTAACA+GGG + tig0032326:22842-22861 Msa1448770:CDS 30.0%
AAGATGAGAAATTGATCTTC+AGG - tig0032326:22957-22976 None:intergenic 30.0%
ACAACTATGATTCATGTAAC+AGG + tig0032326:23115-23134 Msa1448770:intron 30.0%
CAACTATGATTCATGTAACA+GGG + tig0032326:23116-23135 Msa1448770:intron 30.0%
! GAGCTATTATCAATCAGTTT+TGG - tig0032326:23284-23303 None:intergenic 30.0%
! TGATTGATAATAGCTCTCTA+TGG + tig0032326:23288-23307 Msa1448770:CDS 30.0%
AGGTTTCTTTCATTCTTTCT+AGG - tig0032326:23354-23373 None:intergenic 30.0%
!! TGCTAAAACCAAGTATTGAA+TGG - tig0032326:23387-23406 None:intergenic 30.0%
ACATTGTGATCTGATTATGA+AGG + tig0032326:23593-23612 Msa1448770:three_prime_UTR 30.0%
!!! ATTTTGGCTCTTTTTGACAG+AGG - tig0032326:20735-20754 None:intergenic 35.0%
AAGCACATATGGACTCAATA+TGG - tig0032326:20811-20830 None:intergenic 35.0%
! CTTAGCTGTAAAAGCACATA+TGG - tig0032326:20822-20841 None:intergenic 35.0%
ATGTGGATTTAAATCCTCAG+AGG - tig0032326:20867-20886 None:intergenic 35.0%
!! GTTAGTTTGTATCTTGCATG+TGG - tig0032326:20884-20903 None:intergenic 35.0%
CAGAACAGAAAAAGTTACCT+TGG - tig0032326:20979-20998 None:intergenic 35.0%
TGCTATTGTTCTTGTCTTCT+TGG - tig0032326:21114-21133 None:intergenic 35.0%
!! GGAAAGTTGATGGATTGTTA+TGG + tig0032326:21231-21250 Msa1448770:CDS 35.0%
!! TGATGGATTGTTATGGTACA+AGG + tig0032326:21238-21257 Msa1448770:CDS 35.0%
GATTTGGGAAACCATCTTTA+TGG + tig0032326:21260-21279 Msa1448770:CDS 35.0%
TCAAGCAAATAGTTCATTGG+AGG + tig0032326:21304-21323 Msa1448770:CDS 35.0%
CCCAAATAATCAGAACTCAT+AGG - tig0032326:21353-21372 None:intergenic 35.0%
ATAGGTGTTTATGATGGTCA+TGG + tig0032326:21389-21408 Msa1448770:CDS 35.0%
! TGAGAGCATACTCTTGAAAT+TGG - tig0032326:21456-21475 None:intergenic 35.0%
CCAAACATGCACTTAATAGT+TGG - tig0032326:21570-21589 None:intergenic 35.0%
CCAACTATTAAGTGCATGTT+TGG + tig0032326:21567-21586 Msa1448770:intron 35.0%
ATCAAACTATTGCCAACTCT+AGG - tig0032326:21737-21756 None:intergenic 35.0%
GTTAGTACCGCTGTAATTAT+TGG + tig0032326:21813-21832 Msa1448770:intron 35.0%
! GTTTTACAGCTGAAGATCAA+GGG + tig0032326:21889-21908 Msa1448770:CDS 35.0%
AGATCAAGGGATTTCAGAAA+AGG + tig0032326:21902-21921 Msa1448770:CDS 35.0%
ATGCTGTATATTGCAAATGC+AGG + tig0032326:22050-22069 Msa1448770:CDS 35.0%
AAACTGAGAGAGATGATGTT+CGG + tig0032326:22162-22181 Msa1448770:CDS 35.0%
ACCACAATCTGTGAATCATA+GGG - tig0032326:22197-22216 None:intergenic 35.0%
AACCACAATCTGTGAATCAT+AGG - tig0032326:22198-22217 None:intergenic 35.0%
TGGTTATGAAACACTCTGTT+TGG + tig0032326:22213-22232 Msa1448770:CDS 35.0%
CATCATACAGGTACAAACTT+GGG + tig0032326:22247-22266 Msa1448770:intron 35.0%
AGAAAAACATATGCTGGGAA+AGG - tig0032326:22488-22507 None:intergenic 35.0%
! TTTTTCTTATGCAACGGTCA+AGG + tig0032326:22501-22520 Msa1448770:intron 35.0%
ACCTTCAACCAAATGATTAC+GGG + tig0032326:22529-22548 Msa1448770:intron 35.0%
!! AGTTGGTAGCCTTATAATTG+CGG + tig0032326:22620-22639 Msa1448770:intron 35.0%
! GAAAACTCTGATAGTGTTTC+AGG + tig0032326:22661-22680 Msa1448770:intron 35.0%
!! ATTGGCTTGAAAAAGGTTTC+AGG - tig0032326:22894-22913 None:intergenic 35.0%
!! ACTGAGAATTGGCTTGAAAA+AGG - tig0032326:22901-22920 None:intergenic 35.0%
TGAGAAATTGATCTTCAGGT+TGG - tig0032326:22953-22972 None:intergenic 35.0%
TTTCTCATCTTTGCTTCTGA+CGG + tig0032326:22966-22985 Msa1448770:CDS 35.0%
TAACGATATTCACAGCTACT+TGG - tig0032326:23013-23032 None:intergenic 35.0%
! TCACTCCTTGTTCAATCTTT+TGG - tig0032326:23214-23233 None:intergenic 35.0%
AAAAGATTGAACAAGGAGTG+AGG + tig0032326:23213-23232 Msa1448770:CDS 35.0%
CCATAACCATAAACTACTGT+GGG - tig0032326:23458-23477 None:intergenic 35.0%
ACCATAACCATAAACTACTG+TGG - tig0032326:23459-23478 None:intergenic 35.0%
TTGAAACTTGAAGAGTCTCA+TGG + tig0032326:23483-23502 Msa1448770:three_prime_UTR 35.0%
! CTTGTATTTTCTACCACCAT+TGG + tig0032326:23639-23658 Msa1448770:three_prime_UTR 35.0%
AAGCTAAAATATGAGGCCAA+TGG - tig0032326:23658-23677 None:intergenic 35.0%
TATTAGCTCAAACTCAAGCA+AGG + tig0032326:23677-23696 Msa1448770:three_prime_UTR 35.0%
ATTAGCTCAAACTCAAGCAA+GGG + tig0032326:23678-23697 Msa1448770:three_prime_UTR 35.0%
! TCAGAGGTGTGCCATATTAA+AGG - tig0032326:20851-20870 None:intergenic 40.0%
TTTAATATGGCACACCTCTG+AGG + tig0032326:20850-20869 Msa1448770:five_prime_UTR 40.0%
!! AAAAACAAGTTGGTATGGGG+TGG - tig0032326:21007-21026 None:intergenic 40.0%
ATCATGCTGGAAATCAACTG+TGG + tig0032326:21184-21203 Msa1448770:CDS 40.0%
GATGTGAAAGGGAAAGTTGA+TGG + tig0032326:21221-21240 Msa1448770:CDS 40.0%
TCATAGGTCCTGATTCAAGT+TGG - tig0032326:21337-21356 None:intergenic 40.0%
TCTGATTATTTGGGTCCTCA+AGG + tig0032326:21359-21378 Msa1448770:CDS 40.0%
ACACCTATAAAGGTTCCTTG+AGG - tig0032326:21377-21396 None:intergenic 40.0%
! AGATTTGTTTCCTGTCCATG+AGG + tig0032326:21706-21725 Msa1448770:intron 40.0%
GATGTTATAACCTCAAGGGA+CGG + tig0032326:21754-21773 Msa1448770:intron 40.0%
ATGTTATAACCTCAAGGGAC+GGG + tig0032326:21755-21774 Msa1448770:intron 40.0%
! GGTTTTACAGCTGAAGATCA+AGG + tig0032326:21888-21907 Msa1448770:intron 40.0%
! GCATTTTCTGCAACAGATGA+TGG + tig0032326:21936-21955 Msa1448770:CDS 40.0%
TGTCTCTTGTGAAGAAGCAA+TGG + tig0032326:21964-21983 Msa1448770:CDS 40.0%
ATCTGCTGGTACATGTTGTT+TGG + tig0032326:22007-22026 Msa1448770:CDS 40.0%
! TTGGCAGGGATAATTTGCAA+TGG + tig0032326:22026-22045 Msa1448770:CDS 40.0%
TCCCTATGATTCACAGATTG+TGG + tig0032326:22193-22212 Msa1448770:CDS 40.0%
GCATCATACAGGTACAAACT+TGG + tig0032326:22246-22265 Msa1448770:intron 40.0%
TCCCGTAATCATTTGGTTGA+AGG - tig0032326:22533-22552 None:intergenic 40.0%
ACGCGTCAAGGTTTAAATTG+TGG + tig0032326:22552-22571 Msa1448770:intron 40.0%
TTTCTGCGACCGCAATTATA+AGG - tig0032326:22632-22651 None:intergenic 40.0%
TTGCAGGTTTCAAGATCCAT+AGG + tig0032326:22807-22826 Msa1448770:intron 40.0%
CTTCAGATATGCATCACCTA+TGG - tig0032326:22826-22845 None:intergenic 40.0%
TCAGGTAGTCTAAACTTCTG+TGG - tig0032326:22876-22895 None:intergenic 40.0%
TCTTTGCTTCTGACGGTTTA+TGG + tig0032326:22973-22992 Msa1448770:CDS 40.0%
CTTTGCTTCTGACGGTTTAT+GGG + tig0032326:22974-22993 Msa1448770:CDS 40.0%
TCATGTAACAGGGAATAGCA+AGG + tig0032326:23126-23145 Msa1448770:intron 40.0%
GACCTCCAAAAGATTGAACA+AGG + tig0032326:23206-23225 Msa1448770:CDS 40.0%
! CTCCTTGTTCAATCTTTTGG+AGG - tig0032326:23211-23230 None:intergenic 40.0%
TCTCCTCTTTCAATCAGAGA+TGG + tig0032326:23314-23333 Msa1448770:CDS 40.0%
TATTGAATGGATGCTCAGAG+AGG - tig0032326:23374-23393 None:intergenic 40.0%
CTGAGCATCCATTCAATACT+TGG + tig0032326:23376-23395 Msa1448770:three_prime_UTR 40.0%
CCCACAGTAGTTTATGGTTA+TGG + tig0032326:23455-23474 Msa1448770:three_prime_UTR 40.0%
CTAAAATATGAGGCCAATGG+TGG - tig0032326:23655-23674 None:intergenic 40.0%
TGGACTCAATATGGCAGAGT+TGG - tig0032326:20802-20821 None:intergenic 45.0%
! CAGAGAAAACGAAAGCGTGT+TGG - tig0032326:20924-20943 None:intergenic 45.0%
! GCTACTAGTTTCTCATCCCA+AGG + tig0032326:20959-20978 Msa1448770:five_prime_UTR 45.0%
ATGCTGGAAATCAACTGTGG+AGG + tig0032326:21187-21206 Msa1448770:CDS 45.0%
TGCTGGAAATCAACTGTGGA+GGG + tig0032326:21188-21207 Msa1448770:CDS 45.0%
!! GTGGTGATGGAGATGTGAAA+GGG + tig0032326:21210-21229 Msa1448770:CDS 45.0%
GATCGTAGCCAACTTGAATC+AGG + tig0032326:21326-21345 Msa1448770:CDS 45.0%
GGTCCTCAAGGAACCTTTAT+AGG + tig0032326:21371-21390 Msa1448770:CDS 45.0%
GGTGTTTATGATGGTCATGG+TGG + tig0032326:21392-21411 Msa1448770:CDS 45.0%
TACGGCGACTCTTGTGATTA+TGG - tig0032326:21609-21628 None:intergenic 45.0%
AATTTGAATCCCGTCCCTTG+AGG - tig0032326:21767-21786 None:intergenic 45.0%
AAACCACAGATAGCATCTGC+TGG + tig0032326:21993-22012 Msa1448770:CDS 45.0%
! CTGGTACATGTTGTTTGGCA+GGG + tig0032326:22012-22031 Msa1448770:CDS 45.0%
TTGCAAATGCAGGAGACTCA+AGG + tig0032326:22060-22079 Msa1448770:CDS 45.0%
TGCAAATGCAGGAGACTCAA+GGG + tig0032326:22061-22080 Msa1448770:CDS 45.0%
TGTGTTAGGAAGAGTGAGGA+AGG + tig0032326:22085-22104 Msa1448770:CDS 45.0%
GTGTTAGGAAGAGTGAGGAA+GGG + tig0032326:22086-22105 Msa1448770:CDS 45.0%
AAGAGTGAGGAAGGGAACAA+GGG + tig0032326:22094-22113 Msa1448770:CDS 45.0%
AGTCTAAACTTCTGTGGCAG+AGG - tig0032326:22870-22889 None:intergenic 45.0%
GATGGCTCACTACTGAGAAT+TGG - tig0032326:22912-22931 None:intergenic 45.0%
GAAACGAAGCTTACATTGCG+TGG - tig0032326:23044-23063 None:intergenic 45.0%
TGTAACAGGGAATAGCAAGG+AGG + tig0032326:23129-23148 Msa1448770:intron 45.0%
CTGCCATCTCTGATTGAAAG+AGG - tig0032326:23320-23339 None:intergenic 45.0%
CTCTTTCAATCAGAGATGGC+AGG + tig0032326:23318-23337 Msa1448770:CDS 45.0%
CAGTCTCCCACAGTAGTTTA+TGG + tig0032326:23449-23468 Msa1448770:three_prime_UTR 45.0%
TGGAAATCAACTGTGGAGGG+TGG + tig0032326:21191-21210 Msa1448770:CDS 50.0%
!! GGTGGTGATGGAGATGTGAA+AGG + tig0032326:21209-21228 Msa1448770:CDS 50.0%
CAACTCTAGGCAACACCTCA+TGG - tig0032326:21724-21743 None:intergenic 50.0%
!! CATGAGGTGTTGCCTAGAGT+TGG + tig0032326:21722-21741 Msa1448770:intron 50.0%
GTACCAGCAGATGCTATCTG+TGG - tig0032326:21999-22018 None:intergenic 50.0%
! GCTGGTACATGTTGTTTGGC+AGG + tig0032326:22011-22030 Msa1448770:CDS 50.0%
GAAGAGTGAGGAAGGGAACA+AGG + tig0032326:22093-22112 Msa1448770:CDS 50.0%
CACTCTGTTTGGCGTGTGAA+AGG + tig0032326:22224-22243 Msa1448770:CDS 50.0%
!! GCGTGTGAAAGGCATCATAC+AGG + tig0032326:22235-22254 Msa1448770:CDS 50.0%
TGACGCGTCCCGTAATCATT+TGG - tig0032326:22540-22559 None:intergenic 50.0%
AATGATTACGGGACGCGTCA+AGG + tig0032326:22540-22559 Msa1448770:intron 50.0%
TCAACTGTGGAGGGTGGTGA+TGG + tig0032326:21197-21216 Msa1448770:CDS 55.0%
CTAGGCAACACCTCATGGAC+AGG - tig0032326:21719-21738 None:intergenic 55.0%
GGAGACTCAAGGGCTGTGTT+AGG + tig0032326:22071-22090 Msa1448770:CDS 55.0%
GGGCTGTGTTAGGAAGAGTG+AGG + tig0032326:22081-22100 Msa1448770:CDS 55.0%
TAGTCTTCGCAGCTGCGTGC+AGG - tig0032326:22428-22447 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
tig0032326 gene 20664 23742 20664 ID=Msa1448770;Name=Msa1448770
tig0032326 mRNA 20664 23742 20664 ID=Msa1448770-mRNA-1;Parent=Msa1448770;Name=Msa1448770-mRNA-1;_AED=0.03;_eAED=0.03;_QI=424|1|1|1|0.75|0.6|5|387|370
tig0032326 exon 20664 20980 20664 ID=Msa1448770-mRNA-1:exon:16614;Parent=Msa1448770-mRNA-1
tig0032326 exon 21069 21464 21069 ID=Msa1448770-mRNA-1:exon:16615;Parent=Msa1448770-mRNA-1
tig0032326 exon 21889 22256 21889 ID=Msa1448770-mRNA-1:exon:16616;Parent=Msa1448770-mRNA-1
tig0032326 exon 22813 23049 22813 ID=Msa1448770-mRNA-1:exon:16617;Parent=Msa1448770-mRNA-1
tig0032326 exon 23137 23742 23137 ID=Msa1448770-mRNA-1:exon:16618;Parent=Msa1448770-mRNA-1
tig0032326 five_prime_UTR 20664 20980 20664 ID=Msa1448770-mRNA-1:five_prime_utr;Parent=Msa1448770-mRNA-1
tig0032326 five_prime_UTR 21069 21175 21069 ID=Msa1448770-mRNA-1:five_prime_utr;Parent=Msa1448770-mRNA-1
tig0032326 CDS 21176 21464 21176 ID=Msa1448770-mRNA-1:cds;Parent=Msa1448770-mRNA-1
tig0032326 CDS 21889 22256 21889 ID=Msa1448770-mRNA-1:cds;Parent=Msa1448770-mRNA-1
tig0032326 CDS 22813 23049 22813 ID=Msa1448770-mRNA-1:cds;Parent=Msa1448770-mRNA-1
tig0032326 CDS 23137 23355 23137 ID=Msa1448770-mRNA-1:cds;Parent=Msa1448770-mRNA-1
tig0032326 three_prime_UTR 23356 23742 23356 ID=Msa1448770-mRNA-1:three_prime_utr;Parent=Msa1448770-mRNA-1
Gene Sequence

>Msa1448770

ATGACTATATCATGCTGGAAATCAACTGTGGAGGGTGGTGATGGAGATGTGAAAGGGAAAGTTGATGGATTGTTATGGTACAAGGATTTGGGAAACCATCTTTATGGTGAATTTTCAATGGCTGTAATTCAAGCAAATAGTTCATTGGAGGATCGTAGCCAACTTGAATCAGGACCTATGAGTTCTGATTATTTGGGTCCTCAAGGAACCTTTATAGGTGTTTATGATGGTCATGGTGGAACTGCAGCTGCTCAGTTTGTTAATGACAATCTATTCTCCAATTTCAAGAGTTTTACAGCTGAAGATCAAGGGATTTCAGAAAAGGTTATCAAAAGGGCATTTTCTGCAACAGATGATGGTTTTCTGTCTCTTGTGAAGAAGCAATGGCAAAGCAAACCACAGATAGCATCTGCTGGTACATGTTGTTTGGCAGGGATAATTTGCAATGGTATGCTGTATATTGCAAATGCAGGAGACTCAAGGGCTGTGTTAGGAAGAGTGAGGAAGGGAACAAGGGAAACATTAGCTGTTCAGTTATCTACTGAACACAATGTTAATATAGAAACTGAGAGAGATGATGTTCGGTCAAAGCATCCCTATGATTCACAGATTGTGGTTATGAAACACTCTGTTTGGCGTGTGAAAGGCATCATACAGGTTTCAAGATCCATAGGTGATGCATATCTGAAGAAAGCAGAATTTAACAGGGAGCCTCTGCCACAGAAGTTTAGACTACCTGAAACCTTTTTCAAGCCAATTCTCAGTAGTGAGCCATCAATATCAATTCATAAAATCCAACCTGAAGATCAATTTCTCATCTTTGCTTCTGACGGTTTATGGGAGCATCTTAGCAACCAAGTAGCTGTGAATATCGTTAGCAACAATCCACGCAATGGAATAGCAAGGAGGCTTGTGAAAGCAGCACTTAAAGAAGCAGCGAAGAAGCGAGAAATAAGATTATCAGACCTCCAAAAGATTGAACAAGGAGTGAGGAGACACTTCCATGATGATATTACAGTAATTGTTGTATATCTAAATCCAAAACTGATTGATAATAGCTCTCTATGGAGTTCTCCTCTTTCAATCAGAGATGGCAGGCATGTCAATTCCTAG
Protein sequence

>Msa1448770

MTISCWKSTVEGGDGDVKGKVDGLLWYKDLGNHLYGEFSMAVIQANSSLEDRSQLESGPMSSDYLGPQGTFIGVYDGHGGTAAAQFVNDNLFSNFKSFTAEDQGISEKVIKRAFSATDDGFLSLVKKQWQSKPQIASAGTCCLAGIICNGMLYIANAGDSRAVLGRVRKGTRETLAVQLSTEHNVNIETERDDVRSKHPYDSQIVVMKHSVWRVKGIIQVSRSIGDAYLKKAEFNREPLPQKFRLPETFFKPILSSEPSISIHKIQPEDQFLIFASDGLWEHLSNQVAVNIVSNNPRNGIARRLVKAALKEAAKKREIRLSDLQKIEQGVRRHFHDDITVIVVYLNPKLIDNSSLWSSPLSIRDGRHVNS*