AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1240060

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1240060 MtrunA17_Chr8g0352031 98.356 365 6 0 1 365 1 365 0.0 732
Msa1240060 MtrunA17_Chr3g0088301 82.308 390 43 4 1 365 1 389 0.0 615
Msa1240060 MtrunA17_Chr4g0040341 75.134 374 79 5 1 364 1 370 0.0 525
Msa1240060 MtrunA17_Chr2g0328891 73.481 362 87 3 6 365 8 362 3.36e-176 494
Msa1240060 MtrunA17_Chr8g0393031 59.722 288 115 1 67 354 17 303 5.53e-126 365
Msa1240060 MtrunA17_Chr3g0135801 39.604 303 170 7 63 358 29 325 6.25e-69 219
Msa1240060 MtrunA17_Chr3g0135861 30.868 311 178 9 64 352 31 326 8.20e-34 128
Msa1240060 MtrunA17_Chr6g0488081 28.094 299 195 7 64 352 15 303 2.11e-30 118
Msa1240060 MtrunA17_Chr4g0006851 32.281 285 169 8 68 349 89 352 4.87e-28 113
Msa1240060 MtrunA17_Chr8g0347451 28.618 304 179 9 62 339 45 336 4.38e-27 110
Msa1240060 MtrunA17_Chr8g0347451 26.108 203 132 6 71 261 157 353 5.85e-11 63.2
Msa1240060 MtrunA17_Chr5g0396801 28.197 305 167 9 67 339 37 321 5.25e-27 109
Msa1240060 MtrunA17_Chr7g0240341 27.815 302 198 8 65 355 22 314 1.29e-25 105
Msa1240060 MtrunA17_Chr7g0242311 30.634 284 165 9 79 355 98 356 7.24e-25 104
Msa1240060 MtrunA17_Chr1g0149111 29.329 283 171 9 68 344 139 398 9.77e-25 104
Msa1240060 MtrunA17_Chr1g0149111 28.774 212 127 7 56 262 219 411 4.86e-14 72.8
Msa1240060 MtrunA17_Chr3g0137941 27.148 291 186 9 71 356 125 394 2.01e-24 103
Msa1240060 MtrunA17_Chr3g0137941 28.440 218 132 7 56 268 202 400 3.22e-15 76.3
Msa1240060 MtrunA17_Chr7g0252401 29.553 291 161 7 68 344 207 467 3.03e-24 103
Msa1240060 MtrunA17_Chr8g0385571 28.975 283 176 8 68 344 227 490 4.20e-24 103
Msa1240060 MtrunA17_Chr3g0131501 28.269 283 178 8 68 344 205 468 4.69e-23 100
Msa1240060 MtrunA17_Chr5g0436041 24.919 309 210 8 58 355 24 321 6.50e-23 97.8
Msa1240060 MtrunA17_Chr4g0063561 25.000 300 162 7 68 344 123 382 1.71e-22 97.8
Msa1240060 MtrunA17_Chr4g0063561 27.619 210 127 5 58 262 206 395 1.18e-15 77.8
Msa1240060 MtrunA17_Chr4g0067231 25.455 330 198 8 58 351 4 321 2.08e-22 96.7
Msa1240060 MtrunA17_Chr2g0296061 27.476 313 181 9 71 349 18 318 3.30e-22 95.9
Msa1240060 MtrunA17_Chr3g0115801 26.936 297 170 10 71 344 119 391 1.75e-18 86.3
Msa1240060 MtrunA17_Chr5g0411411 27.241 290 187 10 62 339 3 280 1.48e-17 82.0
Msa1240060 MtrunA17_Chr4g0000261 24.503 302 183 8 65 344 63 341 6.89e-17 80.9
Msa1240060 MtrunA17_Chr8g0366791 26.207 290 190 9 62 339 3 280 6.21e-16 77.4
Msa1240060 MtrunA17_Chr4g0004571 27.049 244 163 5 113 349 51 286 7.30e-16 77.4
Msa1240060 MtrunA17_Chr3g0133111 25.418 299 181 10 68 337 6 291 9.71e-15 74.3
Msa1240060 MtrunA17_Chr1g0196921 21.691 272 200 5 63 330 9 271 1.79e-14 73.2
Msa1240060 MtrunA17_Chr2g0331771 24.662 296 200 7 63 349 146 427 2.21e-14 73.9
Msa1240060 MtrunA17_Chr7g0241521 24.453 274 189 7 63 330 12 273 2.49e-14 72.8
Msa1240060 MtrunA17_Chr6g0488341 26.122 245 151 8 116 352 6 228 5.61e-14 70.9
Msa1240060 MtrunA17_Chr4g0008061 24.638 276 186 9 63 330 10 271 3.44e-13 69.7
Msa1240060 MtrunA17_Chr8g0382201 23.232 297 181 9 71 344 198 470 6.60e-13 69.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1240060 AT5G13490.1 79.221 385 60 3 1 365 1 385 0.0 588
Msa1240060 AT5G13490.2 79.221 385 60 3 1 365 1 385 0.0 588
Msa1240060 AT3G08580.2 81.984 383 49 5 1 365 1 381 0.0 575
Msa1240060 AT3G08580.1 81.984 383 49 5 1 365 1 381 0.0 575
Msa1240060 AT4G28390.1 74.406 379 80 5 2 364 1 378 0.0 528
Msa1240060 AT4G28390.2 74.406 379 80 5 2 364 1 378 0.0 528
Msa1240060 AT5G17400.1 62.887 291 107 1 64 354 10 299 7.46e-130 374
Msa1240060 AT5G56450.1 38.710 310 170 7 55 352 21 322 6.56e-67 214
Msa1240060 AT2G37890.1 29.846 325 207 8 43 355 17 332 4.31e-32 123
Msa1240060 AT4G26180.1 31.650 297 171 10 64 344 17 297 1.63e-31 122
Msa1240060 AT3G55640.1 28.041 296 195 7 67 353 37 323 2.28e-31 121
Msa1240060 AT3G55640.1 25.628 199 129 4 63 258 140 322 5.83e-13 69.7
Msa1240060 AT3G55640.4 28.041 296 195 7 67 353 37 323 2.28e-31 121
Msa1240060 AT3G55640.4 25.628 199 129 4 63 258 140 322 5.83e-13 69.7
Msa1240060 AT4G26180.2 31.959 291 166 10 64 338 17 291 9.90e-31 119
Msa1240060 AT1G14560.1 30.605 281 170 7 79 344 38 308 1.76e-30 119
Msa1240060 AT1G14560.2 30.909 275 165 7 79 338 38 302 8.76e-30 116
Msa1240060 AT3G53940.1 30.068 296 186 8 60 344 63 348 2.36e-29 116
Msa1240060 AT3G53940.1 27.619 210 129 5 61 263 169 362 1.71e-13 71.2
Msa1240060 AT3G51870.1 30.986 284 164 9 79 355 102 360 1.92e-26 108
Msa1240060 AT4G01100.1 27.508 309 168 9 71 344 45 332 3.75e-26 107
Msa1240060 AT5G01500.1 29.927 274 168 9 79 349 130 382 5.13e-25 105
Msa1240060 AT4G32400.1 29.078 282 173 9 68 344 114 373 7.85e-25 104
Msa1240060 AT4G01100.2 27.707 314 175 10 71 344 45 346 1.94e-24 103
Msa1240060 AT4G01100.3 26.733 303 166 9 77 344 1 282 5.32e-24 100
Msa1240060 AT5G51050.1 29.537 281 174 7 68 344 223 483 2.22e-23 101
Msa1240060 AT3G21390.1 26.852 324 197 7 58 349 9 324 9.00e-22 95.1
Msa1240060 AT5G61810.2 27.273 297 184 7 52 344 52 320 9.92e-22 95.1
Msa1240060 AT3G21390.2 26.852 324 197 7 58 349 31 346 1.25e-21 95.1
Msa1240060 AT5G61810.1 26.578 301 181 7 52 344 195 463 5.21e-21 94.4
Msa1240060 AT3G20240.1 24.324 296 187 6 67 344 51 327 1.02e-20 92.4
Msa1240060 AT5G07320.1 27.306 271 170 6 77 344 218 464 1.46e-20 93.2
Msa1240060 AT3G55640.2 28.061 196 128 3 67 258 37 223 2.30e-20 89.7
Msa1240060 AT3G55640.3 28.061 196 128 3 67 258 37 223 2.30e-20 89.7
Msa1240060 AT1G14560.3 31.915 188 112 5 172 344 24 210 2.83e-20 89.0
Msa1240060 AT5G48970.1 25.153 326 201 8 58 349 12 328 4.19e-19 87.8
Msa1240060 AT3G53940.2 29.386 228 143 6 60 279 63 280 3.36e-18 84.3
Msa1240060 AT5G46800.2 28.125 288 180 10 67 339 7 282 5.82e-16 78.2
Msa1240060 AT5G46800.3 28.125 288 180 10 67 339 7 282 5.82e-16 78.2
Msa1240060 AT5G46800.1 28.125 288 180 10 67 339 7 282 5.82e-16 78.2
Msa1240060 AT5G64970.1 25.676 296 175 9 71 344 138 410 6.57e-15 75.9
Msa1240060 AT5G64970.2 25.000 296 177 9 71 344 14 286 2.52e-14 73.2
Msa1240060 AT5G64970.3 25.952 289 169 9 71 337 138 403 6.38e-14 72.8
Msa1240060 AT5G48970.2 25.098 255 157 7 121 349 11 257 1.89e-13 70.1
Msa1240060 AT3G54110.1 23.792 269 187 8 68 330 15 271 2.26e-13 70.5
Msa1240060 AT1G14140.1 23.420 269 190 7 68 330 16 274 9.80e-13 68.6
Msa1240060 AT1G14140.1 27.411 197 128 5 173 359 18 209 7.47e-11 63.2
Msa1240060 AT5G58970.1 23.973 292 192 8 58 337 6 279 1.27e-12 68.2
Msa1240060 AT5G66380.1 27.136 199 134 5 166 359 5 197 1.66e-12 67.8
Msa1240060 AT1G79900.1 26.860 242 153 7 115 349 53 277 4.72e-12 66.6
Msa1240060 AT5G01340.1 27.473 182 115 4 59 236 106 274 1.22e-11 65.5
Msa1240060 AT2G33820.1 22.623 305 218 7 64 359 14 309 1.50e-11 65.1
Msa1240060 AT1G78180.1 24.000 300 175 8 71 344 131 403 9.14e-11 63.5

Find 134 sgRNAs with CRISPR-Local

Find 242 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGGTGCTAAGTCCTTGTTTA+AGG 0.144348 8_2:+22313339 Msa1240060:CDS
TAATCATCTCATCCTGGTTT+TGG 0.193782 8_2:-22311836 None:intergenic
TTGAAGCCAGTTCTCCTTAC+TGG 0.211857 8_2:+22312608 Msa1240060:CDS
TTCTTTGCTAGCTTTGGTCT+TGG 0.283922 8_2:+22313181 Msa1240060:CDS
TTTCTATCTATTTACTGTTC+AGG 0.291392 8_2:+22313719 Msa1240060:three_prime_UTR
CAGCTAGAACCAAGTGCTTT+GGG 0.296270 8_2:-22309764 None:intergenic
GAAACTCAAATCAAACAAAA+TGG 0.317317 8_2:-22313672 None:intergenic
GGCTTTGAACTTTGCATTCA+AGG 0.320951 8_2:+22312265 Msa1240060:intron
GCGGGAGCTGCAACAAAGAT+AGG 0.324862 8_2:-22311714 None:intergenic
CTTCTCCTAACTGTGTCAAT+TGG 0.336850 8_2:-22313241 None:intergenic
CCTCGTTCTTGAGGATTTGT+TGG 0.337740 8_2:-22313318 None:intergenic
CCACATTCTGAACGAGATTA+GGG 0.346073 8_2:-22311581 None:intergenic
GATAGTTTCTTTGCTAGCTT+TGG 0.347055 8_2:+22313175 Msa1240060:CDS
ACAAGGATGGTTACTGGAAA+TGG 0.366913 8_2:+22312321 Msa1240060:CDS
GCAGCTAGAACCAAGTGCTT+TGG 0.382444 8_2:-22309765 None:intergenic
AGAAGGACAAGGATGGTTAC+TGG 0.382727 8_2:+22312315 Msa1240060:CDS
CTTTGTTGTTGACTTTCTCA+TGG 0.384159 8_2:+22311749 Msa1240060:CDS
CAAAAGCTTAATTCGTTCAA+TGG 0.387505 8_2:-22311811 None:intergenic
CTGGCAACTGATGGTATTGC+TGG 0.388315 8_2:+22312509 Msa1240060:CDS
ATGTACCAAAGGAGGTCATT+TGG 0.389033 8_2:+22311624 Msa1240060:CDS
CTCCGTGCCGTCGCTGGTGC+TGG 0.395301 8_2:+22313379 Msa1240060:CDS
TTGGCCTCAGGAGGTGCTGC+TGG 0.402435 8_2:+22312356 Msa1240060:CDS
ACAGTCACCGATTCCCTTGT+AGG 0.403293 8_2:-22311880 None:intergenic
CGCTGGTTACGACAAGTTAC+AGG 0.404370 8_2:+22313408 Msa1240060:CDS
AACATCCTCCGTGCCGTCGC+TGG 0.404618 8_2:+22313373 Msa1240060:CDS
GCCACATTCTGAACGAGATT+AGG 0.405088 8_2:-22311582 None:intergenic
TGTACCAAAGGAGGTCATTT+GGG 0.407077 8_2:+22311625 Msa1240060:CDS
GAAAGTCACCATGGTTGATC+AGG 0.415292 8_2:+22311551 Msa1240060:exon
GGTGCTAAGTCCTTGTTTAA+GGG 0.416541 8_2:+22313340 Msa1240060:CDS
GCGTTACATGCTGGCATCAT+TGG 0.421080 8_2:-22311675 None:intergenic
CTTACCCAATTGACACAGTT+AGG 0.421944 8_2:+22313236 Msa1240060:CDS
TTGCTAGCTTTGGTCTTGGA+TGG 0.425017 8_2:+22313185 Msa1240060:CDS
TGGCTCATCACCAATGGTGC+AGG 0.431380 8_2:+22313205 Msa1240060:CDS
TTCTCCTAACTGTGTCAATT+GGG 0.432018 8_2:-22313240 None:intergenic
TGGTGGCTGCGCGAGTTTGT+TGG 0.432992 8_2:-22309804 None:intergenic
GCGCGAGTTTGTTGGAGACT+TGG 0.433533 8_2:-22309796 None:intergenic
GAAATGGTTTGCTGGAAACT+TGG 0.433799 8_2:+22312337 Msa1240060:CDS
CAAGGGAGTAGACAAAGAAA+AGG 0.433806 8_2:-22312388 None:intergenic
AACTCCCAAATGACCTCCTT+TGG 0.434011 8_2:-22311629 None:intergenic
AAAAGCTTAATTCGTTCAAT+GGG 0.436520 8_2:-22311810 None:intergenic
AAGTCCTTGTTTAAGGGAGC+TGG 0.439974 8_2:+22313346 Msa1240060:CDS
CTTTGCATCATTTGCTAGAC+GGG 0.443087 8_2:-22312423 None:intergenic
GGTTACTGGAAATGGTTTGC+TGG 0.444417 8_2:+22312329 Msa1240060:CDS
ATCATTGGATACTGAAATGC+AGG 0.450957 8_2:-22311660 None:intergenic
AGAAGAATGATGATGACATC+TGG 0.454405 8_2:+22313259 Msa1240060:CDS
AGAGATCCAGTAAGGAGAAC+TGG 0.459442 8_2:-22312614 None:intergenic
TGTTCTTCATTTACCCACTA+AGG 0.465015 8_2:+22309884 Msa1240060:five_prime_UTR
AAGCGAACAACGGCCGATGA+AGG 0.465896 8_2:+22311906 Msa1240060:CDS
GTTGTTGCAGCGTTACATGC+TGG 0.471689 8_2:-22311684 None:intergenic
TCTCCTTACTGGATCTCTGC+AGG 0.476142 8_2:+22312619 Msa1240060:CDS
AGATGCTAGCCCTGCACCAT+TGG 0.477482 8_2:-22313215 None:intergenic
GTTGCAGCTCCCGCTGAGAA+AGG 0.477911 8_2:+22311723 Msa1240060:CDS
CGGGAGCTGCAACAAAGATA+GGG 0.484005 8_2:-22311713 None:intergenic
TTGCGGTGTTACCTCTCCAT+AGG 0.487772 8_2:-22311938 None:intergenic
GTGTATCGTGGTCTCTACTT+TGG 0.490767 8_2:+22312572 Msa1240060:CDS
CAGCTTTAATCATCTCATCC+TGG 0.491287 8_2:-22311842 None:intergenic
ATGTAATAGAATGAGAGGTT+AGG 0.492135 8_2:-22309861 None:intergenic
TTTGCTGGAAACTTGGCCTC+AGG 0.497193 8_2:+22312344 Msa1240060:CDS
AGTAAGGAGAACTGGCTTCA+AGG 0.497841 8_2:-22312606 None:intergenic
GTTGTTGACTTTCTCATGGG+TGG 0.497881 8_2:+22311753 Msa1240060:CDS
AGAACTCAACTAACCTGAGT+AGG 0.498897 8_2:-22311978 None:intergenic
TTTGTTGTTGACTTTCTCAT+GGG 0.500591 8_2:+22311750 Msa1240060:CDS
GATGCAAAGGCTGCAAAGAA+AGG 0.508474 8_2:+22312437 Msa1240060:CDS
CATCCGTTATTTCCCTACTC+AGG 0.512437 8_2:+22311965 Msa1240060:CDS
GCTGGTGCTGGTGTGCTCGC+TGG 0.514183 8_2:+22313391 Msa1240060:CDS
ACAAAGTTTCCTTTCTCAGC+GGG 0.514560 8_2:-22311732 None:intergenic
AGATGTTGAAACCACGGTAA+AGG 0.516865 8_2:-22312532 None:intergenic
CCCTAATCTCGTTCAGAATG+TGG 0.518809 8_2:+22311581 Msa1240060:CDS
CAGAATGTGGCACGCCAGAC+CGG 0.519206 8_2:+22311594 Msa1240060:CDS
CTCGGCAAGAAGTACGGATC+CGG 0.520238 8_2:+22313439 Msa1240060:CDS
TAACCTGAGTAGGGAAATAA+CGG 0.524143 8_2:-22311968 None:intergenic
CCTTTGCATCATTTGCTAGA+CGG 0.524216 8_2:-22312424 None:intergenic
TCTGAGCCCTACAAGGGAAT+CGG 0.525862 8_2:+22311873 Msa1240060:CDS
GCTGGAAACTTGGCCTCAGG+AGG 0.526095 8_2:+22312347 Msa1240060:CDS
TGGATACTGAAATGCAGGGT+TGG 0.528389 8_2:-22311655 None:intergenic
TAGACTTTCTGAGCCCTACA+AGG 0.529188 8_2:+22311866 Msa1240060:CDS
ATGAAGGTATTGTCTCCCTA+TGG 0.529311 8_2:+22311922 Msa1240060:CDS
ATAATGCTCGGCAAGAAGTA+CGG 0.537875 8_2:+22313433 Msa1240060:CDS
TGCGGTGTTACCTCTCCATA+GGG 0.537902 8_2:-22311937 None:intergenic
AAGGCTGCAAAGAAAGGTGC+AGG 0.538988 8_2:+22312443 Msa1240060:CDS
CAGTCACCGATTCCCTTGTA+GGG 0.546422 8_2:-22311879 None:intergenic
ACTTAGCACCCTCGTTCTTG+AGG 0.547097 8_2:-22313327 None:intergenic
TAGGGAGACAATACCTTCAT+CGG 0.549059 8_2:-22311919 None:intergenic
GGTTTCAACATCTCTTGTGT+CGG 0.553553 8_2:+22312542 Msa1240060:CDS
AAGGGAGTAGACAAAGAAAA+GGG 0.554552 8_2:-22312387 None:intergenic
TCGTTCAATGGGAGCTGCAG+CGG 0.558742 8_2:-22311799 None:intergenic
GAACTCAACTAACCTGAGTA+GGG 0.561724 8_2:-22311977 None:intergenic
GAAATAACGGATGACATTTG+CGG 0.563798 8_2:-22311955 None:intergenic
CAGGATGAGATGATTAAAGC+TGG 0.564426 8_2:+22311843 Msa1240060:CDS
GAACAATGTAATAGAATGAG+AGG 0.565315 8_2:-22309866 None:intergenic
AACAAAGTTTCCTTTCTCAG+CGG 0.567621 8_2:-22311733 None:intergenic
CACCAGCACCAGCGACGGCA+CGG 0.568903 8_2:-22313381 None:intergenic
GAGCACACCAGCACCAGCGA+CGG 0.570287 8_2:-22313386 None:intergenic
GACGGGTACGAGCATAGTCA+AGG 0.572808 8_2:-22312406 None:intergenic
CTTGGATGGCTCATCACCAA+TGG 0.573594 8_2:+22313199 Msa1240060:CDS
GGTACATAGAAAGACCGGTC+TGG 0.575870 8_2:-22311608 None:intergenic
AAGTTACAGGTCATAATGCT+CGG 0.582137 8_2:+22313421 Msa1240060:CDS
GATTTGTTGGAATGCATCGA+AGG 0.585302 8_2:-22313305 None:intergenic
CATTCAAGGACTACTTCAAG+AGG 0.587339 8_2:+22312279 Msa1240060:CDS
AAAACAAAAGTATGAATCAG+AGG 0.588888 8_2:-22313485 None:intergenic
TGAGAATTGACCAAGTCTTG+CGG 0.597971 8_2:+22313535 Msa1240060:three_prime_UTR
GAAATGAAAATGAGTGTTGG+TGG 0.598283 8_2:-22309821 None:intergenic
AGCTAGAACCAAGTGCTTTG+GGG 0.599462 8_2:-22309763 None:intergenic
CCGGTCTTTCTATGTACCAA+AGG 0.601233 8_2:+22311613 Msa1240060:CDS
CCGTCTAGCAAATGATGCAA+AGG 0.603443 8_2:+22312424 Msa1240060:CDS
GGCTCATCACCAATGGTGCA+GGG 0.604403 8_2:+22313206 Msa1240060:CDS
GAACCTGCAGAGATCCAGTA+AGG 0.606430 8_2:-22312622 None:intergenic
GGCAAGAAGTACGGATCCGG+TGG 0.611623 8_2:+22313442 Msa1240060:CDS
AAGAAGACACTGGCAACTGA+TGG 0.618722 8_2:+22312500 Msa1240060:CDS
AATTTCAAGAAGGACAAGGA+TGG 0.620049 8_2:+22312308 Msa1240060:CDS
AGCACCAGCAGCACCTCCTG+AGG 0.623751 8_2:-22312360 None:intergenic
TTTCAATTTCAAGAAGGACA+AGG 0.627092 8_2:+22312304 Msa1240060:CDS
CCTTTGGTACATAGAAAGAC+CGG 0.627821 8_2:-22311613 None:intergenic
CACAAGAGATGTTGAAACCA+CGG 0.637635 8_2:-22312538 None:intergenic
GGGAGCTGCAACAAAGATAG+GGG 0.642711 8_2:-22311712 None:intergenic
CCAACAAATCCTCAAGAACG+AGG 0.645399 8_2:+22313318 Msa1240060:CDS
GCTGCAAAGAAAGGTGCAGG+AGG 0.645640 8_2:+22312446 Msa1240060:CDS
GCTAGAACCAAGTGCTTTGG+GGG 0.648036 8_2:-22309762 None:intergenic
TAGGAAATGAAAATGAGTGT+TGG 0.653882 8_2:-22309824 None:intergenic
GGTATTGCTGGCCTTTACCG+TGG 0.654046 8_2:+22312521 Msa1240060:CDS
AGACTTTCTGAGCCCTACAA+GGG 0.654879 8_2:+22311867 Msa1240060:CDS
AGCACCAGCTCCCTTAAACA+AGG 0.655144 8_2:-22313350 None:intergenic
GTCGGAATCATTGTGTATCG+TGG 0.657764 8_2:+22312560 Msa1240060:CDS
GGTATTGTCTCCCTATGGAG+AGG 0.663367 8_2:+22311927 Msa1240060:CDS
AAAGCACTTGGTTCTAGCTG+CGG 0.666782 8_2:+22309767 Msa1240060:five_prime_UTR
ACGGGTACGAGCATAGTCAA+GGG 0.668625 8_2:-22312405 None:intergenic
GCAGGAGGAAGACAATTCGA+TGG 0.669093 8_2:+22312461 Msa1240060:CDS
GTCTTTCTATGTACCAAAGG+AGG 0.674751 8_2:+22311616 Msa1240060:CDS
ATTGTTCAGGAAAGTCACCA+TGG 0.678048 8_2:+22311542 Msa1240060:intron
GCGCTGAACCTGATCAACCA+TGG 0.682676 8_2:-22311559 None:intergenic
CAACAAATCCTCAAGAACGA+GGG 0.686427 8_2:+22313319 Msa1240060:CDS
CAGCACCAGCGACGGCACGG+AGG 0.687741 8_2:-22313378 None:intergenic
TGATGTCTACAAGAAGACAC+TGG 0.694165 8_2:+22312490 Msa1240060:CDS
TCATTGGATACTGAAATGCA+GGG 0.721142 8_2:-22311659 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAATGATTAATCAAAAAAT+GGG - chr8_2:22310741-22310760 None:intergenic 10.0%
!!! ATTTTTTGATTAATCATTTA+GGG + chr8_2:22310741-22310760 Msa1240060:intron 10.0%
!!! TCAGAATATTTAATATTTTT+AGG + chr8_2:22313608-22313627 Msa1240060:three_prime_UTR 10.0%
!!! TTTTTTGCAATATGAAAAAA+TGG + chr8_2:22310075-22310094 Msa1240060:intron 15.0%
!! CTAAATGATTAATCAAAAAA+TGG - chr8_2:22310742-22310761 None:intergenic 15.0%
!!! CATTTTTTGATTAATCATTT+AGG + chr8_2:22310740-22310759 Msa1240060:intron 15.0%
!! TTGTTTATGAACTAAATAAT+TGG + chr8_2:22311416-22311435 Msa1240060:intron 15.0%
!! AAAGAACTAAAAAATCAAAA+GGG - chr8_2:22312013-22312032 None:intergenic 15.0%
!!! ATTCTAGTTCATATTAATAT+TGG + chr8_2:22312787-22312806 Msa1240060:intron 15.0%
!!! ACTCTTGTTTTTTATTGAAT+GGG + chr8_2:22310001-22310020 Msa1240060:intron 20.0%
!! CAAATAATGTTCAGAAATTA+AGG - chr8_2:22311506-22311525 None:intergenic 20.0%
!! GAAAGAACTAAAAAATCAAA+AGG - chr8_2:22312014-22312033 None:intergenic 20.0%
!!! TTTTTAGTTCTTTCAAATAG+AGG + chr8_2:22312020-22312039 Msa1240060:intron 20.0%
!!! ATTAATATTGGTGTTATTGA+AGG + chr8_2:22312799-22312818 Msa1240060:intron 20.0%
!! TATCAAAATAGCTAATTCAT+AGG + chr8_2:22313004-22313023 Msa1240060:intron 20.0%
!! AAATATTCTGATCCAAAAAA+GGG - chr8_2:22313599-22313618 None:intergenic 20.0%
!! TAAATATTCTGATCCAAAAA+AGG - chr8_2:22313600-22313619 None:intergenic 20.0%
!!! TTAGGTTTTGTTATTAACCT+AGG - chr8_2:22309846-22309865 None:intergenic 25.0%
! CAAAAACAAAAAACCTTAGT+GGG - chr8_2:22309900-22309919 None:intergenic 25.0%
! ACAAAAACAAAAAACCTTAG+TGG - chr8_2:22309901-22309920 None:intergenic 25.0%
! GCTAAAAAAACAACAACAAA+AGG - chr8_2:22309928-22309947 None:intergenic 25.0%
! TTCATACTGTTGAATTGAAT+TGG + chr8_2:22309954-22309973 Msa1240060:intron 25.0%
!!! CACTCTTGTTTTTTATTGAA+TGG + chr8_2:22310000-22310019 Msa1240060:intron 25.0%
!! ATTAGATCTTTTGTGTATGA+TGG + chr8_2:22310115-22310134 Msa1240060:intron 25.0%
! TGTAGAATGATGAATATGAA+TGG + chr8_2:22310284-22310303 Msa1240060:intron 25.0%
!!! ATAGTGTCTTGTTTGTTTAA+GGG + chr8_2:22310371-22310390 Msa1240060:intron 25.0%
! GAAAAATACTCAAGAAAACA+TGG - chr8_2:22310408-22310427 None:intergenic 25.0%
!!! AAGAATGTTATGGACTATTT+TGG + chr8_2:22310648-22310667 Msa1240060:intron 25.0%
!!! AGAATGTTATGGACTATTTT+GGG + chr8_2:22310649-22310668 Msa1240060:intron 25.0%
!!! TTTGGGTATTATTTTTTGCA+AGG + chr8_2:22310666-22310685 Msa1240060:intron 25.0%
!!! TTGGGTATTATTTTTTGCAA+GGG + chr8_2:22310667-22310686 Msa1240060:intron 25.0%
!!! TTTTTTGCAAGGGATTAAAT+TGG + chr8_2:22310677-22310696 Msa1240060:intron 25.0%
!!! TGATTAATCATTTAGGGTTT+TGG + chr8_2:22310747-22310766 Msa1240060:intron 25.0%
!!! ATTGTTAGGTTTTTGTTTGT+AGG + chr8_2:22310986-22311005 Msa1240060:intron 25.0%
!!! ATTGTTAGGTTTTTGTTTGT+AGG + chr8_2:22310986-22311005 Msa1240060:intron 25.0%
!!! TTTTTGTTTCTGACATTGTT+AGG + chr8_2:22311383-22311402 Msa1240060:intron 25.0%
! AAAAGCTTAATTCGTTCAAT+GGG - chr8_2:22311813-22311832 None:intergenic 25.0%
!!! TCATACTAAATAGAGTATTG+CGG - chr8_2:22312088-22312107 None:intergenic 25.0%
! GAATACATATCAACAAAGTT+AGG - chr8_2:22312771-22312790 None:intergenic 25.0%
!! AAAACAAAAGTATGAATCAG+AGG - chr8_2:22313488-22313507 None:intergenic 25.0%
! GAAACTCAAATCAAACAAAA+TGG - chr8_2:22313675-22313694 None:intergenic 25.0%
! TTTCTATCTATTTACTGTTC+AGG + chr8_2:22313719-22313738 Msa1240060:three_prime_UTR 25.0%
TAGGAAATGAAAATGAGTGT+TGG - chr8_2:22309827-22309846 None:intergenic 30.0%
! AACACTCATTTTCATTTCCT+AGG + chr8_2:22309826-22309845 Msa1240060:five_prime_UTR 30.0%
ATGTAATAGAATGAGAGGTT+AGG - chr8_2:22309864-22309883 None:intergenic 30.0%
GAACAATGTAATAGAATGAG+AGG - chr8_2:22309869-22309888 None:intergenic 30.0%
!! CATAGTGTCTTGTTTGTTTA+AGG + chr8_2:22310370-22310389 Msa1240060:intron 30.0%
!! TAGTGTCTTGTTTGTTTAAG+GGG + chr8_2:22310372-22310391 Msa1240060:intron 30.0%
TGATGAGTGAATACTCAATT+TGG + chr8_2:22310515-22310534 Msa1240060:intron 30.0%
! TCATGACAAGACAATTTTCT+CGG + chr8_2:22310538-22310557 Msa1240060:intron 30.0%
TGTCTCTGTTATAATGAATC+TGG + chr8_2:22310605-22310624 Msa1240060:intron 30.0%
GTCTCTGTTATAATGAATCT+GGG + chr8_2:22310606-22310625 Msa1240060:intron 30.0%
!! TATGTGTTTTCGTCTTGTTA+AGG + chr8_2:22310880-22310899 Msa1240060:intron 30.0%
!! TATGTGTTTTCGTCTTGTTA+AGG + chr8_2:22310880-22310899 Msa1240060:intron 30.0%
! TGTTTTTCTGTTTGTTCTGT+TGG + chr8_2:22311471-22311490 Msa1240060:intron 30.0%
! GTTTTTCTGTTTGTTCTGTT+GGG + chr8_2:22311472-22311491 Msa1240060:intron 30.0%
! TTATGTAACTTGCATTGTTC+AGG + chr8_2:22311529-22311548 Msa1240060:intron 30.0%
!! TTTGTTGTTGACTTTCTCAT+GGG + chr8_2:22311750-22311769 Msa1240060:CDS 30.0%
CAAAAGCTTAATTCGTTCAA+TGG - chr8_2:22311814-22311833 None:intergenic 30.0%
!! TATGAAGTGTTGTCAAATAG+TGG + chr8_2:22312103-22312122 Msa1240060:intron 30.0%
CGATCTATAATTGACAACAA+TGG + chr8_2:22312168-22312187 Msa1240060:intron 30.0%
CTTGTGATTCAAATTCTTCT+AGG + chr8_2:22312244-22312263 Msa1240060:intron 30.0%
TTTCAATTTCAAGAAGGACA+AGG + chr8_2:22312304-22312323 Msa1240060:CDS 30.0%
ACAAAATGACACAGGATATA+AGG - chr8_2:22312722-22312741 None:intergenic 30.0%
GTTAGTCGAACTAAAGAATT+TGG - chr8_2:22312749-22312768 None:intergenic 30.0%
!! TTTTAGTTAATGGCCATGTT+TGG - chr8_2:22313067-22313086 None:intergenic 30.0%
!!! GCTGGCTTTATTTTAGTTAA+TGG - chr8_2:22313077-22313096 None:intergenic 30.0%
AAAAAAGAATTAAGCACCAC+CGG - chr8_2:22313461-22313480 None:intergenic 30.0%
!!! AATGATCCTATACCCTTTTT+TGG + chr8_2:22313584-22313603 Msa1240060:three_prime_UTR 30.0%
!! GAAATGAAAATGAGTGTTGG+TGG - chr8_2:22309824-22309843 None:intergenic 35.0%
TGTTCTTCATTTACCCACTA+AGG + chr8_2:22309884-22309903 Msa1240060:five_prime_UTR 35.0%
! TCTTGAGTATTTTTCCAGCT+TGG + chr8_2:22310413-22310432 Msa1240060:intron 35.0%
AATTCAATCAGAATCCAAGC+TGG - chr8_2:22310430-22310449 None:intergenic 35.0%
! CATGACAAGACAATTTTCTC+GGG + chr8_2:22310539-22310558 Msa1240060:intron 35.0%
! ATGACAAGACAATTTTCTCG+GGG + chr8_2:22310540-22310559 Msa1240060:intron 35.0%
AAAATTGTGAGGGACCAAAT+TGG + chr8_2:22310708-22310727 Msa1240060:intron 35.0%
AAATTGTGAGGGACCAAATT+GGG + chr8_2:22310709-22310728 Msa1240060:intron 35.0%
AAATGGGTAAAGACCCAATT+TGG - chr8_2:22310725-22310744 None:intergenic 35.0%
GAGTTAGAACTTGTGAACTA+TGG + chr8_2:22310829-22310848 Msa1240060:intron 35.0%
CAATGTCAGACTCATGTTTA+TGG - chr8_2:22310970-22310989 None:intergenic 35.0%
AACATGAGTCTGACATTGTT+AGG + chr8_2:22310972-22310991 Msa1240060:intron 35.0%
CAATGTCAGACTCATGTTTA+TGG - chr8_2:22310970-22310989 None:intergenic 35.0%
AACATGAGTCTGACATTGTT+AGG + chr8_2:22310972-22310991 Msa1240060:intron 35.0%
CAATGTCAGAGTCATGTTTA+TGG - chr8_2:22311295-22311314 None:intergenic 35.0%
AACATGACTCTGACATTGTT+AGG + chr8_2:22311297-22311316 Msa1240060:intron 35.0%
AACAATGTCAGAGTCGTTAA+TGG - chr8_2:22311360-22311379 None:intergenic 35.0%
TTAACGACTCTGACATTGTT+AGG + chr8_2:22311360-22311379 Msa1240060:intron 35.0%
GCAAATATCTGAACAAGCAA+TGG - chr8_2:22311449-22311468 None:intergenic 35.0%
TCATTGGATACTGAAATGCA+GGG - chr8_2:22311662-22311681 None:intergenic 35.0%
ATCATTGGATACTGAAATGC+AGG - chr8_2:22311663-22311682 None:intergenic 35.0%
AACAAAGTTTCCTTTCTCAG+CGG - chr8_2:22311736-22311755 None:intergenic 35.0%
!! CTTTGTTGTTGACTTTCTCA+TGG + chr8_2:22311749-22311768 Msa1240060:CDS 35.0%
! TAAGCTTTTGATCCAAAACC+AGG + chr8_2:22311824-22311843 Msa1240060:CDS 35.0%
! TAATCATCTCATCCTGGTTT+TGG - chr8_2:22311839-22311858 None:intergenic 35.0%
!! TGACTGTTTTAAGCGAACAA+CGG + chr8_2:22311896-22311915 Msa1240060:CDS 35.0%
!! GAAATAACGGATGACATTTG+CGG - chr8_2:22311958-22311977 None:intergenic 35.0%
TAACCTGAGTAGGGAAATAA+CGG - chr8_2:22311971-22311990 None:intergenic 35.0%
! TGTCTCTTCACAACTCTTTT+TGG + chr8_2:22312201-22312220 Msa1240060:intron 35.0%
! GAGGCTTTTCAATTTCAAGA+AGG + chr8_2:22312298-22312317 Msa1240060:CDS 35.0%
AATTTCAAGAAGGACAAGGA+TGG + chr8_2:22312308-22312327 Msa1240060:CDS 35.0%
AAGGGAGTAGACAAAGAAAA+GGG - chr8_2:22312390-22312409 None:intergenic 35.0%
ATAAAATGACACAGGGGATA+AGG - chr8_2:22312653-22312672 None:intergenic 35.0%
TGGTCGAATAAAATGACACA+GGG - chr8_2:22312660-22312679 None:intergenic 35.0%
TTGGTCGAATAAAATGACAC+AGG - chr8_2:22312661-22312680 None:intergenic 35.0%
GTTAGTCGAACTAAAGAACT+TGG - chr8_2:22312680-22312699 None:intergenic 35.0%
TTGGTCAAACAAAATGACAC+AGG - chr8_2:22312730-22312749 None:intergenic 35.0%
! AAGCACTTGTGAGACTATTT+TGG + chr8_2:22312861-22312880 Msa1240060:intron 35.0%
!! CTTGTTTTCACAAGCTATCA+TGG - chr8_2:22312942-22312961 None:intergenic 35.0%
TTCAGCTGTTTATCCAAACA+TGG + chr8_2:22313051-22313070 Msa1240060:intron 35.0%
AAAATGAGAAGCAACTTTGC+TGG - chr8_2:22313095-22313114 None:intergenic 35.0%
!! ATTTCCGTTTTGATCATTGC+AGG + chr8_2:22313153-22313172 Msa1240060:intron 35.0%
CTATCCTGCAATGATCAAAA+CGG - chr8_2:22313160-22313179 None:intergenic 35.0%
! GATAGTTTCTTTGCTAGCTT+TGG + chr8_2:22313175-22313194 Msa1240060:CDS 35.0%
TTCTCCTAACTGTGTCAATT+GGG - chr8_2:22313243-22313262 None:intergenic 35.0%
! AGAAGAATGATGATGACATC+TGG + chr8_2:22313259-22313278 Msa1240060:CDS 35.0%
AAGTTACAGGTCATAATGCT+CGG + chr8_2:22313421-22313440 Msa1240060:CDS 35.0%
! ACAAAATTTTCCGCAAGACT+TGG - chr8_2:22313548-22313567 None:intergenic 35.0%
! TCTGATCCAAAAAAGGGTAT+AGG - chr8_2:22313593-22313612 None:intergenic 35.0%
! ATCTGGGACTAATTTGTCCT+CGG + chr8_2:22310622-22310641 Msa1240060:intron 40.0%
TCCTCGGGATAAGAATGTTA+TGG + chr8_2:22310638-22310657 Msa1240060:intron 40.0%
TCCATAACATTCTTATCCCG+AGG - chr8_2:22310642-22310661 None:intergenic 40.0%
TGGTCTGCAAGAAAATTGTG+AGG + chr8_2:22310697-22310716 Msa1240060:intron 40.0%
GGTCTGCAAGAAAATTGTGA+GGG + chr8_2:22310698-22310717 Msa1240060:intron 40.0%
AACATGACTCTGACACTGTT+AGG + chr8_2:22310907-22310926 Msa1240060:intron 40.0%
CAACGTCAGAGTGATGTTTA+TGG - chr8_2:22311035-22311054 None:intergenic 40.0%
AACATGACTCTGACACTGTT+AGG + chr8_2:22310907-22310926 Msa1240060:intron 40.0%
CAACGTCAGAGTGATGTTTA+TGG - chr8_2:22311035-22311054 None:intergenic 40.0%
ATTGTTCAGGAAAGTCACCA+TGG + chr8_2:22311542-22311561 Msa1240060:intron 40.0%
CCACATTCTGAACGAGATTA+GGG - chr8_2:22311584-22311603 None:intergenic 40.0%
CCTTTGGTACATAGAAAGAC+CGG - chr8_2:22311616-22311635 None:intergenic 40.0%
GTCTTTCTATGTACCAAAGG+AGG + chr8_2:22311616-22311635 Msa1240060:CDS 40.0%
ATGTACCAAAGGAGGTCATT+TGG + chr8_2:22311624-22311643 Msa1240060:CDS 40.0%
TGTACCAAAGGAGGTCATTT+GGG + chr8_2:22311625-22311644 Msa1240060:CDS 40.0%
ACAAAGTTTCCTTTCTCAGC+GGG - chr8_2:22311735-22311754 None:intergenic 40.0%
! CAGCTTTAATCATCTCATCC+TGG - chr8_2:22311845-22311864 None:intergenic 40.0%
CAGGATGAGATGATTAAAGC+TGG + chr8_2:22311843-22311862 Msa1240060:CDS 40.0%
TAGGGAGACAATACCTTCAT+CGG - chr8_2:22311922-22311941 None:intergenic 40.0%
!! ATGAAGGTATTGTCTCCCTA+TGG + chr8_2:22311922-22311941 Msa1240060:CDS 40.0%
GAACTCAACTAACCTGAGTA+GGG - chr8_2:22311980-22311999 None:intergenic 40.0%
AGAACTCAACTAACCTGAGT+AGG - chr8_2:22311981-22312000 None:intergenic 40.0%
!! GAAGTGTTGTCAAATAGTGG+CGG + chr8_2:22312106-22312125 Msa1240060:intron 40.0%
!! GGCTTTGAACTTTGCATTCA+AGG + chr8_2:22312265-22312284 Msa1240060:intron 40.0%
CATTCAAGGACTACTTCAAG+AGG + chr8_2:22312279-22312298 Msa1240060:CDS 40.0%
ACAAGGATGGTTACTGGAAA+TGG + chr8_2:22312321-22312340 Msa1240060:CDS 40.0%
!! GAAATGGTTTGCTGGAAACT+TGG + chr8_2:22312337-22312356 Msa1240060:CDS 40.0%
CAAGGGAGTAGACAAAGAAA+AGG - chr8_2:22312391-22312410 None:intergenic 40.0%
! CTTTGCATCATTTGCTAGAC+GGG - chr8_2:22312426-22312445 None:intergenic 40.0%
! CCTTTGCATCATTTGCTAGA+CGG - chr8_2:22312427-22312446 None:intergenic 40.0%
TGATGTCTACAAGAAGACAC+TGG + chr8_2:22312490-22312509 Msa1240060:CDS 40.0%
AGATGTTGAAACCACGGTAA+AGG - chr8_2:22312535-22312554 None:intergenic 40.0%
CACAAGAGATGTTGAAACCA+CGG - chr8_2:22312541-22312560 None:intergenic 40.0%
GGTTTCAACATCTCTTGTGT+CGG + chr8_2:22312542-22312561 Msa1240060:CDS 40.0%
GGTCGAATAAAATGACACAG+GGG - chr8_2:22312659-22312678 None:intergenic 40.0%
!! TTCTTTGCTAGCTTTGGTCT+TGG + chr8_2:22313181-22313200 Msa1240060:CDS 40.0%
CTTACCCAATTGACACAGTT+AGG + chr8_2:22313236-22313255 Msa1240060:CDS 40.0%
CTTCTCCTAACTGTGTCAAT+TGG - chr8_2:22313244-22313263 None:intergenic 40.0%
!! GATTTGTTGGAATGCATCGA+AGG - chr8_2:22313308-22313327 None:intergenic 40.0%
CAACAAATCCTCAAGAACGA+GGG + chr8_2:22313319-22313338 Msa1240060:CDS 40.0%
! GGTGCTAAGTCCTTGTTTAA+GGG + chr8_2:22313340-22313359 Msa1240060:CDS 40.0%
ATAATGCTCGGCAAGAAGTA+CGG + chr8_2:22313433-22313452 Msa1240060:CDS 40.0%
TGAGAATTGACCAAGTCTTG+CGG + chr8_2:22313535-22313554 Msa1240060:three_prime_UTR 40.0%
AGCTAGAACCAAGTGCTTTG+GGG - chr8_2:22309766-22309785 None:intergenic 45.0%
CAGCTAGAACCAAGTGCTTT+GGG - chr8_2:22309767-22309786 None:intergenic 45.0%
!! AAAGCACTTGGTTCTAGCTG+CGG + chr8_2:22309767-22309786 Msa1240060:five_prime_UTR 45.0%
! TCTGGGACTAATTTGTCCTC+GGG + chr8_2:22310623-22310642 Msa1240060:intron 45.0%
GAAAGTCACCATGGTTGATC+AGG + chr8_2:22311551-22311570 Msa1240060:exon 45.0%
CCCTAATCTCGTTCAGAATG+TGG + chr8_2:22311581-22311600 Msa1240060:CDS 45.0%
GCCACATTCTGAACGAGATT+AGG - chr8_2:22311585-22311604 None:intergenic 45.0%
CCGGTCTTTCTATGTACCAA+AGG + chr8_2:22311613-22311632 Msa1240060:CDS 45.0%
AACTCCCAAATGACCTCCTT+TGG - chr8_2:22311632-22311651 None:intergenic 45.0%
TGGATACTGAAATGCAGGGT+TGG - chr8_2:22311658-22311677 None:intergenic 45.0%
!! GTTGTTGACTTTCTCATGGG+TGG + chr8_2:22311753-22311772 Msa1240060:CDS 45.0%
TAGACTTTCTGAGCCCTACA+AGG + chr8_2:22311866-22311885 Msa1240060:CDS 45.0%
AGACTTTCTGAGCCCTACAA+GGG + chr8_2:22311867-22311886 Msa1240060:CDS 45.0%
! CATCCGTTATTTCCCTACTC+AGG + chr8_2:22311965-22311984 Msa1240060:CDS 45.0%
AGAAGGACAAGGATGGTTAC+TGG + chr8_2:22312315-22312334 Msa1240060:CDS 45.0%
! GGTTACTGGAAATGGTTTGC+TGG + chr8_2:22312329-22312348 Msa1240060:CDS 45.0%
CCGTCTAGCAAATGATGCAA+AGG + chr8_2:22312424-22312443 Msa1240060:CDS 45.0%
GATGCAAAGGCTGCAAAGAA+AGG + chr8_2:22312437-22312456 Msa1240060:CDS 45.0%
AAGAAGACACTGGCAACTGA+TGG + chr8_2:22312500-22312519 Msa1240060:CDS 45.0%
GTCGGAATCATTGTGTATCG+TGG + chr8_2:22312560-22312579 Msa1240060:CDS 45.0%
GTGTATCGTGGTCTCTACTT+TGG + chr8_2:22312572-22312591 Msa1240060:CDS 45.0%
! AGTAAGGAGAACTGGCTTCA+AGG - chr8_2:22312609-22312628 None:intergenic 45.0%
TTGAAGCCAGTTCTCCTTAC+TGG + chr8_2:22312608-22312627 Msa1240060:CDS 45.0%
AGAGATCCAGTAAGGAGAAC+TGG - chr8_2:22312617-22312636 None:intergenic 45.0%
!! TTGCTAGCTTTGGTCTTGGA+TGG + chr8_2:22313185-22313204 Msa1240060:CDS 45.0%
CCTCGTTCTTGAGGATTTGT+TGG - chr8_2:22313321-22313340 None:intergenic 45.0%
CCAACAAATCCTCAAGAACG+AGG + chr8_2:22313318-22313337 Msa1240060:CDS 45.0%
! GGGTGCTAAGTCCTTGTTTA+AGG + chr8_2:22313339-22313358 Msa1240060:CDS 45.0%
! AAGTCCTTGTTTAAGGGAGC+TGG + chr8_2:22313346-22313365 Msa1240060:CDS 45.0%
GCTAGAACCAAGTGCTTTGG+GGG - chr8_2:22309765-22309784 None:intergenic 50.0%
GCAGCTAGAACCAAGTGCTT+TGG - chr8_2:22309768-22309787 None:intergenic 50.0%
GGTACATAGAAAGACCGGTC+TGG - chr8_2:22311611-22311630 None:intergenic 50.0%
GCGTTACATGCTGGCATCAT+TGG - chr8_2:22311678-22311697 None:intergenic 50.0%
GTTGTTGCAGCGTTACATGC+TGG - chr8_2:22311687-22311706 None:intergenic 50.0%
GGGAGCTGCAACAAAGATAG+GGG - chr8_2:22311715-22311734 None:intergenic 50.0%
CGGGAGCTGCAACAAAGATA+GGG - chr8_2:22311716-22311735 None:intergenic 50.0%
TCTGAGCCCTACAAGGGAAT+CGG + chr8_2:22311873-22311892 Msa1240060:CDS 50.0%
!! CAGTCACCGATTCCCTTGTA+GGG - chr8_2:22311882-22311901 None:intergenic 50.0%
!! ACAGTCACCGATTCCCTTGT+AGG - chr8_2:22311883-22311902 None:intergenic 50.0%
GGTATTGTCTCCCTATGGAG+AGG + chr8_2:22311927-22311946 Msa1240060:CDS 50.0%
!! TGCGGTGTTACCTCTCCATA+GGG - chr8_2:22311940-22311959 None:intergenic 50.0%
!! TTGCGGTGTTACCTCTCCAT+AGG - chr8_2:22311941-22311960 None:intergenic 50.0%
TTGTCAAATAGTGGCGGCAC+TGG + chr8_2:22312112-22312131 Msa1240060:intron 50.0%
TGTCAAATAGTGGCGGCACT+GGG + chr8_2:22312113-22312132 Msa1240060:intron 50.0%
TTTGCTGGAAACTTGGCCTC+AGG + chr8_2:22312344-22312363 Msa1240060:CDS 50.0%
ACGGGTACGAGCATAGTCAA+GGG - chr8_2:22312408-22312427 None:intergenic 50.0%
! AAGGCTGCAAAGAAAGGTGC+AGG + chr8_2:22312443-22312462 Msa1240060:CDS 50.0%
GCAGGAGGAAGACAATTCGA+TGG + chr8_2:22312461-22312480 Msa1240060:CDS 50.0%
CTGGCAACTGATGGTATTGC+TGG + chr8_2:22312509-22312528 Msa1240060:CDS 50.0%
TCTCCTTACTGGATCTCTGC+AGG + chr8_2:22312619-22312638 Msa1240060:CDS 50.0%
GAACCTGCAGAGATCCAGTA+AGG - chr8_2:22312625-22312644 None:intergenic 50.0%
CTTGGATGGCTCATCACCAA+TGG + chr8_2:22313199-22313218 Msa1240060:CDS 50.0%
ACTTAGCACCCTCGTTCTTG+AGG - chr8_2:22313330-22313349 None:intergenic 50.0%
AGCACCAGCTCCCTTAAACA+AGG - chr8_2:22313353-22313372 None:intergenic 50.0%
! CGCTGGTTACGACAAGTTAC+AGG + chr8_2:22313408-22313427 Msa1240060:CDS 50.0%
!! GCGCGAGTTTGTTGGAGACT+TGG - chr8_2:22309799-22309818 None:intergenic 55.0%
GCGCTGAACCTGATCAACCA+TGG - chr8_2:22311562-22311581 None:intergenic 55.0%
GCGGGAGCTGCAACAAAGAT+AGG - chr8_2:22311717-22311736 None:intergenic 55.0%
TCGTTCAATGGGAGCTGCAG+CGG - chr8_2:22311802-22311821 None:intergenic 55.0%
AAGCGAACAACGGCCGATGA+AGG + chr8_2:22311906-22311925 Msa1240060:CDS 55.0%
GACGGGTACGAGCATAGTCA+AGG - chr8_2:22312409-22312428 None:intergenic 55.0%
! GCTGCAAAGAAAGGTGCAGG+AGG + chr8_2:22312446-22312465 Msa1240060:CDS 55.0%
!! GGTATTGCTGGCCTTTACCG+TGG + chr8_2:22312521-22312540 Msa1240060:CDS 55.0%
TGGCTCATCACCAATGGTGC+AGG + chr8_2:22313205-22313224 Msa1240060:CDS 55.0%
GGCTCATCACCAATGGTGCA+GGG + chr8_2:22313206-22313225 Msa1240060:CDS 55.0%
AGATGCTAGCCCTGCACCAT+TGG - chr8_2:22313218-22313237 None:intergenic 55.0%
! CTCGGCAAGAAGTACGGATC+CGG + chr8_2:22313439-22313458 Msa1240060:CDS 55.0%
! CTCTCAGCCCCCAAAGCACT+TGG + chr8_2:22309755-22309774 Msa1240060:five_prime_UTR 60.0%
TGGTGGCTGCGCGAGTTTGT+TGG - chr8_2:22309807-22309826 None:intergenic 60.0%
CAGAATGTGGCACGCCAGAC+CGG + chr8_2:22311594-22311613 Msa1240060:CDS 60.0%
GTTGCAGCTCCCGCTGAGAA+AGG + chr8_2:22311723-22311742 Msa1240060:CDS 60.0%
GCTGGAAACTTGGCCTCAGG+AGG + chr8_2:22312347-22312366 Msa1240060:CDS 60.0%
! GGCAAGAAGTACGGATCCGG+TGG + chr8_2:22313442-22313461 Msa1240060:CDS 60.0%
!! TTGGCCTCAGGAGGTGCTGC+TGG + chr8_2:22312356-22312375 Msa1240060:CDS 65.0%
AGCACCAGCAGCACCTCCTG+AGG - chr8_2:22312363-22312382 None:intergenic 65.0%
AACATCCTCCGTGCCGTCGC+TGG + chr8_2:22313373-22313392 Msa1240060:CDS 65.0%
GAGCACACCAGCACCAGCGA+CGG - chr8_2:22313389-22313408 None:intergenic 65.0%
CACCAGCACCAGCGACGGCA+CGG - chr8_2:22313384-22313403 None:intergenic 70.0%
!! GCTGGTGCTGGTGTGCTCGC+TGG + chr8_2:22313391-22313410 Msa1240060:CDS 70.0%
CAGCACCAGCGACGGCACGG+AGG - chr8_2:22313381-22313400 None:intergenic 75.0%
CTCCGTGCCGTCGCTGGTGC+TGG + chr8_2:22313379-22313398 Msa1240060:CDS 75.0%
Chromosome Type Strat End Strand Name
chr8_2 gene 22309753 22313819 22309753 ID=Msa1240060;Name=Msa1240060
chr8_2 mRNA 22309753 22313819 22309753 ID=Msa1240060-mRNA-1;Parent=Msa1240060;Name=Msa1240060-mRNA-1;_AED=0.10;_eAED=0.10;_QI=163|1|1|1|0.66|0.75|4|348|365
chr8_2 exon 22309753 22309905 22309753 ID=Msa1240060-mRNA-1:exon:7934;Parent=Msa1240060-mRNA-1
chr8_2 exon 22311551 22311986 22311551 ID=Msa1240060-mRNA-1:exon:7935;Parent=Msa1240060-mRNA-1
chr8_2 exon 22312266 22312640 22312266 ID=Msa1240060-mRNA-1:exon:7936;Parent=Msa1240060-mRNA-1
chr8_2 exon 22313175 22313819 22313175 ID=Msa1240060-mRNA-1:exon:7937;Parent=Msa1240060-mRNA-1
chr8_2 five_prime_UTR 22309753 22309905 22309753 ID=Msa1240060-mRNA-1:five_prime_utr;Parent=Msa1240060-mRNA-1
chr8_2 five_prime_UTR 22311551 22311560 22311551 ID=Msa1240060-mRNA-1:five_prime_utr;Parent=Msa1240060-mRNA-1
chr8_2 CDS 22311561 22311986 22311561 ID=Msa1240060-mRNA-1:cds;Parent=Msa1240060-mRNA-1
chr8_2 CDS 22312266 22312640 22312266 ID=Msa1240060-mRNA-1:cds;Parent=Msa1240060-mRNA-1
chr8_2 CDS 22313175 22313471 22313175 ID=Msa1240060-mRNA-1:cds;Parent=Msa1240060-mRNA-1
chr8_2 three_prime_UTR 22313472 22313819 22313472 ID=Msa1240060-mRNA-1:three_prime_utr;Parent=Msa1240060-mRNA-1
Gene Sequence

>Msa1240060

ATGGTTGATCAGGTTCAGCGCCCTAATCTCGTTCAGAATGTGGCACGCCAGACCGGTCTTTCTATGTACCAAAGGAGGTCATTTGGGAGTTACTCCAACCCTGCATTTCAGTATCCAATGATGCCAGCATGTAACGCTGCAACAACTGCTTCCCCTATCTTTGTTGCAGCTCCCGCTGAGAAAGGAAACTTTGTTGTTGACTTTCTCATGGGTGGAGTTTCAGCTGCTGTATCAAAAACCGCTGCAGCTCCCATTGAACGAATTAAGCTTTTGATCCAAAACCAGGATGAGATGATTAAAGCTGGTAGACTTTCTGAGCCCTACAAGGGAATCGGTGACTGTTTTAAGCGAACAACGGCCGATGAAGGTATTGTCTCCCTATGGAGAGGTAACACCGCAAATGTCATCCGTTATTTCCCTACTCAGGCTTTGAACTTTGCATTCAAGGACTACTTCAAGAGGCTTTTCAATTTCAAGAAGGACAAGGATGGTTACTGGAAATGGTTTGCTGGAAACTTGGCCTCAGGAGGTGCTGCTGGTGCTTCATCCCTTTTCTTTGTCTACTCCCTTGACTATGCTCGTACCCGTCTAGCAAATGATGCAAAGGCTGCAAAGAAAGGTGCAGGAGGAAGACAATTCGATGGTCTTATTGATGTCTACAAGAAGACACTGGCAACTGATGGTATTGCTGGCCTTTACCGTGGTTTCAACATCTCTTGTGTCGGAATCATTGTGTATCGTGGTCTCTACTTTGGAATGTATGATTCCTTGAAGCCAGTTCTCCTTACTGGATCTCTGCAGGATAGTTTCTTTGCTAGCTTTGGTCTTGGATGGCTCATCACCAATGGTGCAGGGCTAGCATCTTACCCAATTGACACAGTTAGGAGAAGAATGATGATGACATCTGGAGAAGCTGTCAAGTACAAGAGCTCCTTCGATGCATTCCAACAAATCCTCAAGAACGAGGGTGCTAAGTCCTTGTTTAAGGGAGCTGGTGCTAACATCCTCCGTGCCGTCGCTGGTGCTGGTGTGCTCGCTGGTTACGACAAGTTACAGGTCATAATGCTCGGCAAGAAGTACGGATCCGGTGGTGCTTAA
Protein sequence

>Msa1240060

MVDQVQRPNLVQNVARQTGLSMYQRRSFGSYSNPAFQYPMMPACNAATTASPIFVAAPAEKGNFVVDFLMGGVSAAVSKTAAAPIERIKLLIQNQDEMIKAGRLSEPYKGIGDCFKRTTADEGIVSLWRGNTANVIRYFPTQALNFAFKDYFKRLFNFKKDKDGYWKWFAGNLASGGAAGASSLFFVYSLDYARTRLANDAKAAKKGAGGRQFDGLIDVYKKTLATDGIAGLYRGFNISCVGIIVYRGLYFGMYDSLKPVLLTGSLQDSFFASFGLGWLITNGAGLASYPIDTVRRRMMMTSGEAVKYKSSFDAFQQILKNEGAKSLFKGAGANILRAVAGAGVLAGYDKLQVIMLGKKYGSGGA*