AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0508430

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0508430 MtrunA17_Chr3g0112791 91.705 217 14 1 1 213 1 217 6.19e-143 397
Msa0508430 MtrunA17_Chr5g0436201 58.019 212 60 7 26 213 5 211 9.57e-72 217
Msa0508430 MtrunA17_Chr7g0240961 56.627 166 54 7 53 209 74 230 1.18e-50 164
Msa0508430 MtrunA17_Chr3g0112811 69.466 131 30 5 10 138 1 123 7.68e-48 153
Msa0508430 MtrunA17_Chr8g0379191 34.498 229 119 9 3 211 92 309 7.35e-29 109
Msa0508430 MtrunA17_Chr4g0045171 32.889 225 128 7 3 211 81 298 2.55e-28 108
Msa0508430 MtrunA17_Chr2g0284111 40.000 135 61 3 84 207 153 278 5.80e-28 107
Msa0508430 MtrunA17_Chr3g0120281 42.188 64 28 1 87 150 240 294 4.76e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0508430 AT5G05830.1 53.226 186 67 6 40 213 26 203 5.25e-51 164
Msa0508430 AT5G01070.1 50.735 136 55 4 84 213 75 204 3.42e-37 129
Msa0508430 AT2G37950.1 45.865 133 61 2 84 213 81 205 2.31e-35 124
Msa0508430 AT5G59000.1 40.132 152 59 3 86 209 80 227 5.05e-27 103
Msa0508430 AT2G34200.2 37.209 129 71 4 84 210 99 219 3.98e-19 82.4
Msa0508430 AT2G34200.1 37.209 129 71 4 84 210 99 219 3.98e-19 82.4
Msa0508430 AT5G60580.1 43.750 64 27 1 87 150 257 311 5.59e-11 61.6
Msa0508430 AT5G60580.3 43.750 64 27 1 87 150 257 311 5.59e-11 61.6
Msa0508430 AT5G60580.4 43.750 64 27 1 87 150 257 311 5.63e-11 61.6
Msa0508430 AT5G60580.2 43.750 64 27 1 87 150 257 311 5.63e-11 61.6

Find 72 sgRNAs with CRISPR-Local

Find 204 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCTTATTGTCTTCTCATT+TGG 0.163355 3_4:-56400244 Msa0508430:three_prime_UTR
TCCTGAAGTTATAGGATTTC+AGG 0.175642 3_4:+56400549 None:intergenic
CTTCTTTCAACAAACCTTAA+TGG 0.186312 3_4:-56403179 Msa0508430:five_prime_UTR
CTGCTAGATCCTGAAGTTAT+AGG 0.214776 3_4:+56400541 None:intergenic
CCAGCTTCCAAACATAATTT+AGG 0.216231 3_4:-56400352 Msa0508430:three_prime_UTR
TTCTTATTGTCTTCTCATTT+GGG 0.244244 3_4:-56400243 Msa0508430:three_prime_UTR
TCCTCTCTCACACGACTTCT+AGG 0.262476 3_4:+56403291 None:intergenic
ATGTGCTGAAGCTTGGTTTA+AGG 0.266567 3_4:-56402773 Msa0508430:CDS
TGTTAATGCAGTTATTAATT+TGG 0.282539 3_4:+56403255 None:intergenic
GAGATCATGAATACCCATTA+AGG 0.284798 3_4:+56403165 None:intergenic
TTCTTTCAACAAACCTTAAT+GGG 0.300039 3_4:-56403178 Msa0508430:five_prime_UTR
AATGTGAATAAATCAATTTA+AGG 0.329367 3_4:+56403330 None:intergenic
AATCTTCATGTCATGGCTTT+GGG 0.337486 3_4:+56403137 None:intergenic
GAATCTTCATGTCATGGCTT+TGG 0.342745 3_4:+56403136 None:intergenic
GAATTTCCTCCTTGCCTGTA+TGG 0.353899 3_4:-56400617 Msa0508430:CDS
CTTCTCATTTGGGTTATGAA+TGG 0.367231 3_4:-56400233 Msa0508430:three_prime_UTR
GATACTTCCTAAATTATGTT+TGG 0.383113 3_4:+56400345 None:intergenic
GATGATGACCAACATGAATC+TGG 0.390187 3_4:-56402853 Msa0508430:CDS
TCATTGCCTTTGTCATCTCC+TGG 0.395813 3_4:-56400594 Msa0508430:CDS
TCCATTGCTAGAAATGTATC+TGG 0.396063 3_4:-56400754 Msa0508430:CDS
TCCTGAAATCCTATAACTTC+AGG 0.402202 3_4:-56400550 Msa0508430:exon
ACACGAACATCCTAGTTCAA+TGG 0.404206 3_4:+56402825 None:intergenic
TAGGATTTGTCATCTCAGTA+TGG 0.410659 3_4:-56402890 Msa0508430:CDS
TTGTTGAAAGAAGAAGAAAA+AGG 0.424162 3_4:+56403189 None:intergenic
CCTAAATTATGTTTGGAAGC+TGG 0.430685 3_4:+56400352 None:intergenic
CACGAACATCCTAGTTCAAT+GGG 0.431600 3_4:+56402826 None:intergenic
CATGGTACCTGTGTTTATAA+TGG 0.435036 3_4:+56400483 None:intergenic
GTAAGATGATTGAGGATTTG+TGG 0.435692 3_4:-56400518 Msa0508430:three_prime_UTR
AAGTTATAACTTATAATAAC+AGG 0.436688 3_4:+56400286 None:intergenic
GGCGCTGAGTTTCTGCAGGT+GGG 0.443589 3_4:+56400656 None:intergenic
CGCTGAGTTTCTGCAGGTGG+GGG 0.452158 3_4:+56400658 None:intergenic
GTCTGAATGTTGTGTGGATG+TGG 0.458451 3_4:-56402959 Msa0508430:CDS
ATGTTGTGTGGATGTGGATC+TGG 0.464923 3_4:-56402953 Msa0508430:CDS
ATAAACAATGTGCTGAAGCT+TGG 0.473834 3_4:-56402780 Msa0508430:CDS
ATGTTCGTGTAAAGATGATT+TGG 0.486734 3_4:-56402812 Msa0508430:CDS
ATGGTGTGAAGAGAGATTGT+AGG 0.503740 3_4:-56402909 Msa0508430:CDS
TGAAGCTTGGTTTAAGGTCA+AGG 0.518133 3_4:-56402767 Msa0508430:CDS
AGGCGCTGAGTTTCTGCAGG+TGG 0.521622 3_4:+56400655 None:intergenic
ACCATCGAAAATGTCCTTCA+CGG 0.522737 3_4:-56403102 Msa0508430:CDS
CCATCGAAAATGTCCTTCAC+GGG 0.523616 3_4:-56403101 Msa0508430:CDS
ACCAGATACATTTCTAGCAA+TGG 0.523936 3_4:+56400753 None:intergenic
TGTTCAAGTAAGTAACAAGA+AGG 0.538413 3_4:-56400418 Msa0508430:three_prime_UTR
GCGCTGAGTTTCTGCAGGTG+GGG 0.539720 3_4:+56400657 None:intergenic
AGTTGAGAATCTTCATGTCA+TGG 0.547212 3_4:+56403130 None:intergenic
CTATAACTTCAGGATCTAGC+AGG 0.548028 3_4:-56400540 Msa0508430:three_prime_UTR
TCTGGGACTCCCATTGAACT+AGG 0.554284 3_4:-56402835 Msa0508430:CDS
TCCTAGAAGTCGTGTGAGAG+AGG 0.555404 3_4:-56403292 Msa0508430:five_prime_UTR
GACAAAGGCAATGACCATAC+AGG 0.555573 3_4:+56400603 None:intergenic
TAACTCTACAGCGGAGAATG+TGG 0.555921 3_4:-56403019 Msa0508430:CDS
GTCTGTGTCTGAATGTTGTG+TGG 0.557366 3_4:-56402965 Msa0508430:CDS
AACTCTACAGCGGAGAATGT+GGG 0.566964 3_4:-56403018 Msa0508430:CDS
GGATGTGGATCTGGAGAGTG+TGG 0.567109 3_4:-56402944 Msa0508430:CDS
AAACTCAGCGCCTACTCCAC+CGG 0.567388 3_4:-56400645 Msa0508430:CDS
AGTAGGCGCTGAGTTTCTGC+AGG 0.570474 3_4:+56400652 None:intergenic
TCTAGCAGGTAAGATGATTG+AGG 0.586586 3_4:-56400526 Msa0508430:three_prime_UTR
ATTGAGACTGCATTGTGTTG+AGG 0.593238 3_4:-56400186 Msa0508430:three_prime_UTR
ATGATGACCAACATGAATCT+GGG 0.596167 3_4:-56402852 Msa0508430:CDS
TACAGAACTTGTGAAATCTG+TGG 0.602282 3_4:-56400778 Msa0508430:intron
AATAAGAAGCAGTTGAGACA+AGG 0.603597 3_4:+56400258 None:intergenic
AGTGTGGTTAATGTTGATGA+TGG 0.605562 3_4:-56402928 Msa0508430:CDS
GAAGCTTGGTTTAAGGTCAA+GGG 0.608837 3_4:-56402766 Msa0508430:CDS
GTTCAAGTAAGTAACAAGAA+GGG 0.613575 3_4:-56400417 Msa0508430:three_prime_UTR
TGGGAGTCCCAGATTCATGT+TGG 0.638328 3_4:+56402845 None:intergenic
TCTGTGACCATTATAAACAC+AGG 0.659282 3_4:-56400490 Msa0508430:three_prime_UTR
AGGCAATGACCATACAGGCA+AGG 0.660733 3_4:+56400608 None:intergenic
CATCGAAAATGTCCTTCACG+GGG 0.665927 3_4:-56403100 Msa0508430:CDS
CAATGACCATACAGGCAAGG+AGG 0.693990 3_4:+56400611 None:intergenic
AAGTTGAAATGACAGAACAG+TGG 0.697944 3_4:-56400723 Msa0508430:CDS
ATCTCCTCGTAACTCTACAG+CGG 0.714391 3_4:-56403028 Msa0508430:CDS
TTCTCCGCTGTAGAGTTACG+AGG 0.717423 3_4:+56403024 None:intergenic
AGAGACGCGTGAATTGAACA+TGG 0.743408 3_4:+56400465 None:intergenic
ATCGAAAATGTCCTTCACGG+GGG 0.766428 3_4:-56403099 Msa0508430:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATTAAAAAAAAAATGACT+AGG - chr3_4:56401245-56401264 Msa0508430:intron 10.0%
!! AATGTGAATAAATCAATTTA+AGG + chr3_4:56400188-56400207 None:intergenic 15.0%
!!! ATTGATTTATTCACATTTTT+TGG - chr3_4:56400191-56400210 Msa0508430:three_prime_UTR 15.0%
!!! ATTAACATTGTTTCTTAATT+AGG - chr3_4:56400797-56400816 Msa0508430:intron 15.0%
!! TCAAAAATCAAGTAAATTAT+TGG - chr3_4:56401358-56401377 Msa0508430:intron 15.0%
!! CAAAAATCAAGTAAATTATT+GGG - chr3_4:56401359-56401378 Msa0508430:intron 15.0%
!!! TTTTAGAAAAACATGTTATA+TGG + chr3_4:56401913-56401932 None:intergenic 15.0%
!!! TTAATTCTTTTATTCCATTT+TGG - chr3_4:56402225-56402244 Msa0508430:intron 15.0%
!!! TAATTCTTTTATTCCATTTT+GGG - chr3_4:56402226-56402245 Msa0508430:intron 15.0%
!!! AGTTAACTGTTTTATTTTTT+TGG - chr3_4:56402686-56402705 Msa0508430:intron 15.0%
!! AAGTTATAACTTATAATAAC+AGG + chr3_4:56403232-56403251 None:intergenic 15.0%
!! TGTTAATGCAGTTATTAATT+TGG + chr3_4:56400263-56400282 None:intergenic 20.0%
!!! TGATTTTTGAATAATAGAAC+AGG + chr3_4:56401348-56401367 None:intergenic 20.0%
!!! AAATTAAGTTGTTTATCCAA+AGG - chr3_4:56401643-56401662 Msa0508430:intron 20.0%
!!! CACTTTTTGTGTTTAAATTA+CGG - chr3_4:56401886-56401905 Msa0508430:intron 20.0%
!!! GTTAAACTACTATAATCTTT+TGG - chr3_4:56402280-56402299 Msa0508430:intron 20.0%
!!! CATTATCTTATTTAATAGAC+TGG - chr3_4:56402304-56402323 Msa0508430:intron 20.0%
!!! TATTTATAGAAGTATAAGGA+TGG + chr3_4:56402619-56402638 None:intergenic 20.0%
!!! GAAGTATTTATAGAAGTATA+AGG + chr3_4:56402623-56402642 None:intergenic 20.0%
!! CTTCTATAAATACTTCTAAT+TGG - chr3_4:56402627-56402646 Msa0508430:intron 20.0%
! TTGTTGAAAGAAGAAGAAAA+AGG + chr3_4:56400329-56400348 None:intergenic 25.0%
! TTCTTTCAACAAACCTTAAT+GGG - chr3_4:56400337-56400356 Msa0508430:three_prime_UTR 25.0%
! TTTCTTAATTAGGTGTGTAA+TGG - chr3_4:56400807-56400826 Msa0508430:intron 25.0%
!!! CAAAGCATATCATTTTTATC+TGG + chr3_4:56401160-56401179 None:intergenic 25.0%
!! TTGTGTAGTAAATAGTTTGT+AGG - chr3_4:56401177-56401196 Msa0508430:intron 25.0%
! TATCATAATTGTGCTTGAAT+TGG + chr3_4:56401204-56401223 None:intergenic 25.0%
!! TTTTTGAATAATAGAACAGG+AGG + chr3_4:56401345-56401364 None:intergenic 25.0%
!! GTTTTCAGCTTATCTTTATA+AGG - chr3_4:56401524-56401543 Msa0508430:intron 25.0%
! TTATCTTTATAAGGTCTCAA+GGG - chr3_4:56401533-56401552 Msa0508430:intron 25.0%
!! TAAACAAATGTTTCTACCTT+TGG + chr3_4:56401662-56401681 None:intergenic 25.0%
!!! TTTGCTTTTATGTGATGTTA+CGG - chr3_4:56401807-56401826 Msa0508430:intron 25.0%
! CTGCGATATAAAAACATTTA+GGG + chr3_4:56402081-56402100 None:intergenic 25.0%
! GAAAGTCTGAAAATTGATAA+TGG - chr3_4:56402188-56402207 Msa0508430:intron 25.0%
! CCAAATTTACATGAATCAAA+AGG + chr3_4:56402369-56402388 None:intergenic 25.0%
!!! CCTTTTGATTCATGTAAATT+TGG - chr3_4:56402366-56402385 Msa0508430:intron 25.0%
! TGTAAATTTGGAAAACATCT+TGG - chr3_4:56402378-56402397 Msa0508430:intron 25.0%
!!! TGTGGCTGAATTTTTTATAT+TGG - chr3_4:56402408-56402427 Msa0508430:intron 25.0%
!!! TGCTGCAAAAATATTTTTGT+TGG - chr3_4:56403125-56403144 Msa0508430:CDS 25.0%
!!! TGCAAAAATATTTTTGTTGG+TGG - chr3_4:56403128-56403147 Msa0508430:CDS 25.0%
! GATACTTCCTAAATTATGTT+TGG + chr3_4:56403173-56403192 None:intergenic 25.0%
! TTCTTATTGTCTTCTCATTT+GGG - chr3_4:56403272-56403291 Msa0508430:five_prime_UTR 25.0%
CTTCTTTCAACAAACCTTAA+TGG - chr3_4:56400336-56400355 Msa0508430:three_prime_UTR 30.0%
ATGTTCGTGTAAAGATGATT+TGG - chr3_4:56400703-56400722 Msa0508430:CDS 30.0%
AATGGGTATAGACGAATATA+AGG - chr3_4:56401402-56401421 Msa0508430:intron 30.0%
CTTATCTTTATAAGGTCTCA+AGG - chr3_4:56401532-56401551 Msa0508430:intron 30.0%
!! ACAACTTAATTTGCAGCTTA+TGG + chr3_4:56401635-56401654 None:intergenic 30.0%
AACAATATGAGAGCTTACAA+AGG + chr3_4:56401763-56401782 None:intergenic 30.0%
CATATTGTTTCCCTATTAGA+AGG - chr3_4:56401774-56401793 Msa0508430:intron 30.0%
GATTCTACTTCCTTCTAATA+GGG + chr3_4:56401787-56401806 None:intergenic 30.0%
AGATTCTACTTCCTTCTAAT+AGG + chr3_4:56401788-56401807 None:intergenic 30.0%
! GAAACAGTTTAGTCTTCTAA+GGG - chr3_4:56401829-56401848 Msa0508430:intron 30.0%
! AAAAACATTTAGGGTGTGTT+TGG + chr3_4:56402072-56402091 None:intergenic 30.0%
CCTGCGATATAAAAACATTT+AGG + chr3_4:56402082-56402101 None:intergenic 30.0%
!! CCTAAATGTTTTTATATCGC+AGG - chr3_4:56402079-56402098 Msa0508430:intron 30.0%
AGTTGAATCAAATCACAACT+AGG + chr3_4:56402164-56402183 None:intergenic 30.0%
!!! CTTTTATTCCATTTTGGGTT+TGG - chr3_4:56402231-56402250 Msa0508430:intron 30.0%
! CAAAAGGTTACTCTTTTCTA+TGG + chr3_4:56402353-56402372 None:intergenic 30.0%
!!! GCTGAATTTTTTATATTGGC+TGG - chr3_4:56402412-56402431 Msa0508430:intron 30.0%
! GCAATATTTTACCATCAAAC+AGG + chr3_4:56402457-56402476 None:intergenic 30.0%
ATGCTTGAATTCACATACTA+TGG + chr3_4:56402508-56402527 None:intergenic 30.0%
!!! ATTTGTCACTTTTCTACCTT+TGG + chr3_4:56402578-56402597 None:intergenic 30.0%
! AAAAGTGACAAATGTTGGTT+AGG - chr3_4:56402585-56402604 Msa0508430:intron 30.0%
TGTTCAAGTAAGTAACAAGA+AGG - chr3_4:56403097-56403116 Msa0508430:CDS 30.0%
GTTCAAGTAAGTAACAAGAA+GGG - chr3_4:56403098-56403117 Msa0508430:CDS 30.0%
! TCATATTTTTCACAACATGC+AGG - chr3_4:56403193-56403212 Msa0508430:five_prime_UTR 30.0%
CTTCTTATTGTCTTCTCATT+TGG - chr3_4:56403271-56403290 Msa0508430:five_prime_UTR 30.0%
GAGATCATGAATACCCATTA+AGG + chr3_4:56400353-56400372 None:intergenic 35.0%
AATCTTCATGTCATGGCTTT+GGG + chr3_4:56400381-56400400 None:intergenic 35.0%
AGTTGAGAATCTTCATGTCA+TGG + chr3_4:56400388-56400407 None:intergenic 35.0%
! TGCATTTTCGATATTGTCGA+CGG + chr3_4:56400468-56400487 None:intergenic 35.0%
! AGTGTGGTTAATGTTGATGA+TGG - chr3_4:56400587-56400606 Msa0508430:CDS 35.0%
TAGGATTTGTCATCTCAGTA+TGG - chr3_4:56400625-56400644 Msa0508430:CDS 35.0%
ATGATGACCAACATGAATCT+GGG - chr3_4:56400663-56400682 Msa0508430:CDS 35.0%
ATAAACAATGTGCTGAAGCT+TGG - chr3_4:56400735-56400754 Msa0508430:CDS 35.0%
!! TGTAGCATTGACACTTATGA+TGG - chr3_4:56400891-56400910 Msa0508430:intron 35.0%
ACTCACTATTAGTGCTTCAT+AGG - chr3_4:56401006-56401025 Msa0508430:intron 35.0%
ACATGAGTGACTCAAATAGT+AGG + chr3_4:56401041-56401060 None:intergenic 35.0%
TGACATGTGCTGAATATTCT+CGG - chr3_4:56401060-56401079 Msa0508430:intron 35.0%
AATATTCTCGGAGGTTTAGA+TGG - chr3_4:56401072-56401091 Msa0508430:intron 35.0%
ACTAGATTGAGACATGCTAT+TGG - chr3_4:56401121-56401140 Msa0508430:intron 35.0%
! TACCCATTTTACCCTTTTCT+GGG + chr3_4:56401390-56401409 None:intergenic 35.0%
! ATACCCATTTTACCCTTTTC+TGG + chr3_4:56401391-56401410 None:intergenic 35.0%
ATGATTGTCTGAGAGAACTT+AGG - chr3_4:56401473-56401492 Msa0508430:intron 35.0%
TTTGTTTAGATAACCAGAGC+CGG - chr3_4:56401674-56401693 Msa0508430:intron 35.0%
TATCAAACATGATGTGCACT+GGG + chr3_4:56401724-56401743 None:intergenic 35.0%
ATATCAAACATGATGTGCAC+TGG + chr3_4:56401725-56401744 None:intergenic 35.0%
! GGAAACAGTTTAGTCTTCTA+AGG - chr3_4:56401828-56401847 Msa0508430:intron 35.0%
! TGTGTTTGGATTGATGGTAT+AGG - chr3_4:56401852-56401871 Msa0508430:intron 35.0%
! TTTGGATTGATGGTATAGGA+GGG - chr3_4:56401856-56401875 Msa0508430:intron 35.0%
TTTATATCGCAGGTACTTAG+AGG - chr3_4:56402089-56402108 Msa0508430:intron 35.0%
! TCCAACTTTTATGTGACAGT+TGG + chr3_4:56402330-56402349 None:intergenic 35.0%
AAACATCTTGGAAACTCTTG+TGG - chr3_4:56402390-56402409 Msa0508430:intron 35.0%
ATTGGCTGGATTAATTCTCA+TGG - chr3_4:56402426-56402445 Msa0508430:intron 35.0%
AAGTAGTTGTTCAAGTCCAA+AGG - chr3_4:56402559-56402578 Msa0508430:intron 35.0%
GGTAGAAAAGTGACAAATGT+TGG - chr3_4:56402580-56402599 Msa0508430:intron 35.0%
! ATAGAAGTATAAGGATGGCT+TGG + chr3_4:56402614-56402633 None:intergenic 35.0%
TACAGAACTTGTGAAATCTG+TGG - chr3_4:56402737-56402756 Msa0508430:intron 35.0%
TCCATTGCTAGAAATGTATC+TGG - chr3_4:56402761-56402780 Msa0508430:CDS 35.0%
!! ACCAGATACATTTCTAGCAA+TGG + chr3_4:56402765-56402784 None:intergenic 35.0%
AAGTTGAAATGACAGAACAG+TGG - chr3_4:56402792-56402811 Msa0508430:CDS 35.0%
CAAGGAGGAAATTCAAAAAC+CGG + chr3_4:56402892-56402911 None:intergenic 35.0%
TCCTGAAATCCTATAACTTC+AGG - chr3_4:56402965-56402984 Msa0508430:CDS 35.0%
TCCTGAAGTTATAGGATTTC+AGG + chr3_4:56402969-56402988 None:intergenic 35.0%
GTAAGATGATTGAGGATTTG+TGG - chr3_4:56402997-56403016 Msa0508430:CDS 35.0%
TCTGTGACCATTATAAACAC+AGG - chr3_4:56403025-56403044 Msa0508430:CDS 35.0%
CATGGTACCTGTGTTTATAA+TGG + chr3_4:56403035-56403054 None:intergenic 35.0%
CCTAAATTATGTTTGGAAGC+TGG + chr3_4:56403166-56403185 None:intergenic 35.0%
CCAGCTTCCAAACATAATTT+AGG - chr3_4:56403163-56403182 Msa0508430:exon 35.0%
AATAAGAAGCAGTTGAGACA+AGG + chr3_4:56403260-56403279 None:intergenic 35.0%
! CTTCTCATTTGGGTTATGAA+TGG - chr3_4:56403282-56403301 Msa0508430:five_prime_UTR 35.0%
GAATCTTCATGTCATGGCTT+TGG + chr3_4:56400382-56400401 None:intergenic 40.0%
ACCATCGAAAATGTCCTTCA+CGG - chr3_4:56400413-56400432 Msa0508430:three_prime_UTR 40.0%
! GCATTTTCGATATTGTCGAC+GGG + chr3_4:56400467-56400486 None:intergenic 40.0%
ATGGTGTGAAGAGAGATTGT+AGG - chr3_4:56400606-56400625 Msa0508430:CDS 40.0%
GATGATGACCAACATGAATC+TGG - chr3_4:56400662-56400681 Msa0508430:CDS 40.0%
CACGAACATCCTAGTTCAAT+GGG + chr3_4:56400692-56400711 None:intergenic 40.0%
ACACGAACATCCTAGTTCAA+TGG + chr3_4:56400693-56400712 None:intergenic 40.0%
ATGTGCTGAAGCTTGGTTTA+AGG - chr3_4:56400742-56400761 Msa0508430:CDS 40.0%
! TGAAGCTTGGTTTAAGGTCA+AGG - chr3_4:56400748-56400767 Msa0508430:CDS 40.0%
! GAAGCTTGGTTTAAGGTCAA+GGG - chr3_4:56400749-56400768 Msa0508430:CDS 40.0%
AAGTGAAACTTGCAAGATGC+AGG + chr3_4:56401315-56401334 None:intergenic 40.0%
TGTCCCAGAAAAGGGTAAAA+TGG - chr3_4:56401384-56401403 Msa0508430:intron 40.0%
GTCCCAGAAAAGGGTAAAAT+GGG - chr3_4:56401385-56401404 Msa0508430:intron 40.0%
TTGTTTAGATAACCAGAGCC+GGG - chr3_4:56401675-56401694 Msa0508430:intron 40.0%
! CTTTACTGAATTTTCGTCCC+CGG + chr3_4:56401696-56401715 None:intergenic 40.0%
! TAGTCTTCTAAGGGTGTGTT+TGG - chr3_4:56401838-56401857 Msa0508430:intron 40.0%
! TAAGGGTGTGTTTGGATTGA+TGG - chr3_4:56401846-56401865 Msa0508430:intron 40.0%
! GTTTGGATTGATGGTATAGG+AGG - chr3_4:56401855-56401874 Msa0508430:intron 40.0%
! TTGGATTGATGGTATAGGAG+GGG - chr3_4:56401857-56401876 Msa0508430:intron 40.0%
! ACATTTAGGGTGTGTTTGGA+TGG + chr3_4:56402068-56402087 None:intergenic 40.0%
ATGGACTTCATCTGGAGAAT+GGG - chr3_4:56402112-56402131 Msa0508430:intron 40.0%
GCCAACTGTCACATAAAAGT+TGG - chr3_4:56402326-56402345 Msa0508430:intron 40.0%
! TCATGGCTTTACCTGTTTGA+TGG - chr3_4:56402443-56402462 Msa0508430:intron 40.0%
GTGAATTCAAGCATGTACTG+CGG - chr3_4:56402514-56402533 Msa0508430:intron 40.0%
AATTCAAAAACCGGTGGAGT+AGG + chr3_4:56402883-56402902 None:intergenic 40.0%
AAAAAGCCAGGAGATGACAA+AGG + chr3_4:56402930-56402949 None:intergenic 40.0%
CAAATAGAAGCGAAAAAGCC+AGG + chr3_4:56402942-56402961 None:intergenic 40.0%
CTGCTAGATCCTGAAGTTAT+AGG + chr3_4:56402977-56402996 None:intergenic 40.0%
CTATAACTTCAGGATCTAGC+AGG - chr3_4:56402975-56402994 Msa0508430:CDS 40.0%
TCTAGCAGGTAAGATGATTG+AGG - chr3_4:56402989-56403008 Msa0508430:CDS 40.0%
ATTGAGACTGCATTGTGTTG+AGG - chr3_4:56403329-56403348 Msa0508430:five_prime_UTR 40.0%
CCATCGAAAATGTCCTTCAC+GGG - chr3_4:56400414-56400433 Msa0508430:three_prime_UTR 45.0%
CATCGAAAATGTCCTTCACG+GGG - chr3_4:56400415-56400434 Msa0508430:three_prime_UTR 45.0%
ATCGAAAATGTCCTTCACGG+GGG - chr3_4:56400416-56400435 Msa0508430:three_prime_UTR 45.0%
ATCTCCTCGTAACTCTACAG+CGG - chr3_4:56400487-56400506 Msa0508430:three_prime_UTR 45.0%
TAACTCTACAGCGGAGAATG+TGG - chr3_4:56400496-56400515 Msa0508430:three_prime_UTR 45.0%
AACTCTACAGCGGAGAATGT+GGG - chr3_4:56400497-56400516 Msa0508430:three_prime_UTR 45.0%
GTCTGTGTCTGAATGTTGTG+TGG - chr3_4:56400550-56400569 Msa0508430:exon 45.0%
GTCTGAATGTTGTGTGGATG+TGG - chr3_4:56400556-56400575 Msa0508430:exon 45.0%
ATGTTGTGTGGATGTGGATC+TGG - chr3_4:56400562-56400581 Msa0508430:exon 45.0%
CACATGTGTCAGTGTTGTGT+CGG + chr3_4:56400926-56400945 None:intergenic 45.0%
CATGTGCTGAATATTCTCGG+AGG - chr3_4:56401063-56401082 Msa0508430:intron 45.0%
AGGTGCATCAGCATTTGCAT+AGG - chr3_4:56401265-56401284 Msa0508430:intron 45.0%
TATTGGGTGTGTCCCAGAAA+AGG - chr3_4:56401375-56401394 Msa0508430:intron 45.0%
ATTGGGTGTGTCCCAGAAAA+GGG - chr3_4:56401376-56401395 Msa0508430:intron 45.0%
TGTTTAGATAACCAGAGCCG+GGG - chr3_4:56401676-56401695 Msa0508430:intron 45.0%
CGGGGACGAAAATTCAGTAA+AGG - chr3_4:56401694-56401713 Msa0508430:intron 45.0%
! ATTGATGGTATAGGAGGGGA+AGG - chr3_4:56401861-56401880 Msa0508430:intron 45.0%
TTTGGATGGAGGGTTTAGGA+GGG + chr3_4:56402054-56402073 None:intergenic 45.0%
TGTGTTTGGATGGAGGGTTT+AGG + chr3_4:56402058-56402077 None:intergenic 45.0%
TTAGGGTGTGTTTGGATGGA+GGG + chr3_4:56402064-56402083 None:intergenic 45.0%
! TTTAGGGTGTGTTTGGATGG+AGG + chr3_4:56402065-56402084 None:intergenic 45.0%
TATCGCAGGTACTTAGAGGA+TGG - chr3_4:56402093-56402112 Msa0508430:intron 45.0%
! CTTAGAGGATGGACTTCATC+TGG - chr3_4:56402104-56402123 Msa0508430:intron 45.0%
! GATGGACTTCATCTGGAGAA+TGG - chr3_4:56402111-56402130 Msa0508430:intron 45.0%
GGTCAACTCCAAACCCAAAA+TGG + chr3_4:56402242-56402261 None:intergenic 45.0%
AACAGAGCAGAGTGCACAAT+TGG + chr3_4:56402263-56402282 None:intergenic 45.0%
GGAGGAAATTCAAAAACCGG+TGG + chr3_4:56402889-56402908 None:intergenic 45.0%
GAATTTCCTCCTTGCCTGTA+TGG - chr3_4:56402898-56402917 Msa0508430:CDS 45.0%
GACAAAGGCAATGACCATAC+AGG + chr3_4:56402915-56402934 None:intergenic 45.0%
TCATTGCCTTTGTCATCTCC+TGG - chr3_4:56402921-56402940 Msa0508430:CDS 45.0%
AGAGACGCGTGAATTGAACA+TGG + chr3_4:56403053-56403072 None:intergenic 45.0%
!!! TTTTTAATTATTTAGAAAAA+GGG + chr3_4:56401235-56401254 None:intergenic 5.0%
!!! TTTTTTAATTATTTAGAAAA+AGG + chr3_4:56401236-56401255 None:intergenic 5.0%
TCCTAGAAGTCGTGTGAGAG+AGG - chr3_4:56400223-56400242 Msa0508430:three_prime_UTR 50.0%
TCCTCTCTCACACGACTTCT+AGG + chr3_4:56400227-56400246 None:intergenic 50.0%
! CCCGTGAAGGACATTTTCGA+TGG + chr3_4:56400417-56400436 None:intergenic 50.0%
TTCTCCGCTGTAGAGTTACG+AGG + chr3_4:56400494-56400513 None:intergenic 50.0%
TGGGAGTCCCAGATTCATGT+TGG + chr3_4:56400673-56400692 None:intergenic 50.0%
! TCTGGGACTCCCATTGAACT+AGG - chr3_4:56400680-56400699 Msa0508430:CDS 50.0%
TTGGATGGAGGGTTTAGGAG+GGG + chr3_4:56402053-56402072 None:intergenic 50.0%
GTTTGGATGGAGGGTTTAGG+AGG + chr3_4:56402055-56402074 None:intergenic 50.0%
CAATGACCATACAGGCAAGG+AGG + chr3_4:56402907-56402926 None:intergenic 50.0%
AGGCAATGACCATACAGGCA+AGG + chr3_4:56402910-56402929 None:intergenic 50.0%
ACTTGTCTTGTCCCCCGTGA+AGG + chr3_4:56400430-56400449 None:intergenic 55.0%
GGATGTGGATCTGGAGAGTG+TGG - chr3_4:56400571-56400590 Msa0508430:CDS 55.0%
! TGAATTTTCGTCCCCGGCTC+TGG + chr3_4:56401690-56401709 None:intergenic 55.0%
TGGATGGAGGGTTTAGGAGG+GGG + chr3_4:56402052-56402071 None:intergenic 55.0%
!! AGTAGGCGCTGAGTTTCTGC+AGG + chr3_4:56402866-56402885 None:intergenic 55.0%
AAACTCAGCGCCTACTCCAC+CGG - chr3_4:56402870-56402889 Msa0508430:CDS 55.0%
TGGAGGGTTTAGGAGGGGGA+GGG + chr3_4:56402048-56402067 None:intergenic 60.0%
ATGGAGGGTTTAGGAGGGGG+AGG + chr3_4:56402049-56402068 None:intergenic 60.0%
TGAGTTTCTGCAGGTGGGGG+AGG + chr3_4:56402857-56402876 None:intergenic 60.0%
CGCTGAGTTTCTGCAGGTGG+GGG + chr3_4:56402860-56402879 None:intergenic 60.0%
GCGCTGAGTTTCTGCAGGTG+GGG + chr3_4:56402861-56402880 None:intergenic 60.0%
! GGCGCTGAGTTTCTGCAGGT+GGG + chr3_4:56402862-56402881 None:intergenic 60.0%
!! AGGCGCTGAGTTTCTGCAGG+TGG + chr3_4:56402863-56402882 None:intergenic 60.0%
GGTTTAGGAGGGGGAGGGAG+GGG + chr3_4:56402043-56402062 None:intergenic 65.0%
GGGTTTAGGAGGGGGAGGGA+GGG + chr3_4:56402044-56402063 None:intergenic 65.0%
AGGGTTTAGGAGGGGGAGGG+AGG + chr3_4:56402045-56402064 None:intergenic 65.0%
GGTGGGGGAGGTCTAGTTGG+TGG + chr3_4:56402845-56402864 None:intergenic 65.0%
GCAGGTGGGGGAGGTCTAGT+TGG + chr3_4:56402848-56402867 None:intergenic 65.0%
TAGGAGGGGGAGGGAGGGGA+GGG + chr3_4:56402039-56402058 None:intergenic 70.0%
TTAGGAGGGGGAGGGAGGGG+AGG + chr3_4:56402040-56402059 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr3_4 gene 56400161 56403376 56400161 ID=Msa0508430;Name=Msa0508430
chr3_4 mRNA 56400161 56403376 56400161 ID=Msa0508430-mRNA-1;Parent=Msa0508430;Name=Msa0508430-mRNA-1;_AED=0.00;_eAED=0.00;_QI=204|1|1|1|1|1|2|406|213
chr3_4 exon 56400161 56400795 56400161 ID=Msa0508430-mRNA-1:exon:12726;Parent=Msa0508430-mRNA-1
chr3_4 exon 56402760 56403376 56402760 ID=Msa0508430-mRNA-1:exon:12725;Parent=Msa0508430-mRNA-1
chr3_4 five_prime_UTR 56403173 56403376 56403173 ID=Msa0508430-mRNA-1:five_prime_utr;Parent=Msa0508430-mRNA-1
chr3_4 CDS 56402760 56403172 56402760 ID=Msa0508430-mRNA-1:cds;Parent=Msa0508430-mRNA-1
chr3_4 CDS 56400567 56400795 56400567 ID=Msa0508430-mRNA-1:cds;Parent=Msa0508430-mRNA-1
chr3_4 three_prime_UTR 56400161 56400566 56400161 ID=Msa0508430-mRNA-1:three_prime_utr;Parent=Msa0508430-mRNA-1
Gene Sequence

>Msa0508430

ATGATCTCTATCTCCCAAAGCCATGACATGAAGATTCTCAACTTTAGTACCATCGAAAATGTCCTTCACGGGGGACAAGACAAGTCTCACGTTGTTGTTCCCGTCGACAATATCGAAAATGCATCTCCTCGTAACTCTACAGCGGAGAATGTGGGAGTTTGTGAGAAAGAGAGAGTGTCTTCTGTGTCTGTGTCTGAATGTTGTGTGGATGTGGATCTGGAGAGTGTGGTTAATGTTGATGATGGTGTGAAGAGAGATTGTAGGATTTGTCATCTCAGTATGGATATGAGAAATCGTGATGATGACCAACATGAATCTGGGACTCCCATTGAACTAGGATGTTCGTGTAAAGATGATTTGGCTGCTGCTCATAAACAATGTGCTGAAGCTTGGTTTAAGGTCAAGGGTAACAAAACTTGTGAAATCTGTGGATCCATTGCTAGAAATGTATCTGGTATAGTTGAAGTTGAAATGACAGAACAGTGGAGTGAAGCTAATGATGCATCTGTTCTACCACCAACTAGACCTCCCCCACCTGCAGAAACTCAGCGCCTACTCCACCGGTTTTTGAATTTCCTCCTTGCCTGTATGGTCATTGCCTTTGTCATCTCCTGGCTTTTTCGCTTCTATTTGCATTCCTGA
Protein sequence

>Msa0508430

MISISQSHDMKILNFSTIENVLHGGQDKSHVVVPVDNIENASPRNSTAENVGVCEKERVSSVSVSECCVDVDLESVVNVDDGVKRDCRICHLSMDMRNRDDDQHESGTPIELGCSCKDDLAAAHKQCAEAWFKVKGNKTCEICGSIARNVSGIVEVEMTEQWSEANDASVLPPTRPPPPAETQRLLHRFLNFLLACMVIAFVISWLFRFYLHS*