AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0523570

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0523570 MtrunA17_Chr3g0144451 96.602 206 7 0 1 206 1 206 3.87e-148 413
Msa0523570 MtrunA17_Chr3g0138231 83.092 207 24 2 1 206 1 197 7.18e-115 328
Msa0523570 MtrunA17_Chr4g0034821 64.055 217 67 2 1 206 6 222 3.07e-90 267
Msa0523570 MtrunA17_Chr3g0144421 43.421 228 100 4 7 206 7 233 8.94e-56 179
Msa0523570 MtrunA17_Chr3g0144391 46.667 150 77 1 57 206 290 436 5.99e-41 145
Msa0523570 MtrunA17_Chr3g0144411 46.000 150 78 1 57 206 323 469 3.93e-40 143
Msa0523570 MtrunA17_Chr4g0034451 39.645 169 98 2 39 206 290 455 3.75e-37 135
Msa0523570 MtrunA17_Chr4g0034731 43.919 148 80 2 59 206 217 361 1.44e-35 129
Msa0523570 MtrunA17_Chr4g0034721 46.622 148 76 2 59 206 361 505 3.23e-34 127
Msa0523570 MtrunA17_Chr4g0034691 41.104 163 90 2 47 206 478 637 2.52e-33 125
Msa0523570 MtrunA17_Chr6g0485051 45.161 124 67 1 84 206 11 134 2.69e-29 108
Msa0523570 MtrunA17_Chr1g0147271 38.961 154 90 3 54 206 166 316 3.46e-28 109
Msa0523570 MtrunA17_Chr1g0147291 39.216 153 86 4 55 206 52 198 3.49e-26 101
Msa0523570 MtrunA17_Chr1g0147321 39.216 153 86 4 55 206 23 169 3.54e-26 101
Msa0523570 MtrunA17_Chr1g0147301 38.312 154 90 4 55 206 66 216 1.83e-25 100
Msa0523570 MtrunA17_Chr1g0147241 37.013 154 92 2 53 206 96 244 1.83e-25 100
Msa0523570 MtrunA17_Chr1g0147251 37.500 152 87 3 55 206 31 174 3.00e-25 98.6
Msa0523570 MtrunA17_Chr1g0147331 37.662 154 90 4 54 206 108 256 4.34e-25 100
Msa0523570 MtrunA17_Chr3g0139431 37.419 155 92 3 53 206 101 251 1.06e-24 99.0
Msa0523570 MtrunA17_Chr1g0147311 38.312 154 90 4 55 206 137 287 1.14e-24 99.4
Msa0523570 MtrunA17_Chr1g0146631 37.013 154 91 4 54 206 108 256 4.19e-24 97.4
Msa0523570 MtrunA17_Chr1g0147261 37.500 152 90 3 55 206 85 231 5.37e-24 96.7
Msa0523570 MtrunA17_Chr8g0365361 29.487 156 90 4 59 206 181 324 9.46e-13 66.2
Msa0523570 MtrunA17_Chr3g0117791 33.036 112 72 1 95 206 448 556 8.56e-11 60.8
Msa0523570 MtrunA17_Chr5g0413591 27.632 152 98 4 59 206 415 558 9.24e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0523570 AT1G49320.1 35.714 210 124 4 1 206 7 209 7.32e-39 135
Msa0523570 AT5G25610.1 39.744 156 90 4 53 206 170 323 6.58e-27 106

Find 73 sgRNAs with CRISPR-Local

Find 167 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTCTTCACTTTGAAAGATT+TGG 0.265422 3_4:+76974872 Msa0523570:CDS
TTGAAAAGTAACACTTGATT+TGG 0.297099 3_4:-76975207 None:intergenic
GAATTTACACAAAACATTCT+TGG 0.298406 3_4:+76975143 Msa0523570:CDS
AAGTGATTCAGCTTCTTCTT+TGG 0.321679 3_4:-76974961 None:intergenic
AGTGATTCAGCTTCTTCTTT+GGG 0.342654 3_4:-76974960 None:intergenic
CTTCTTTGGGCCATAACTTA+GGG 0.345605 3_4:-76974947 None:intergenic
TTATTTGAAGTTGCGGTTGC+CGG 0.346213 3_4:-76975368 None:intergenic
GAACTTAACCTCTCCTTTGA+TGG 0.356006 3_4:-76975093 None:intergenic
GTGTGACAAGCCACCATCTT+TGG 0.368463 3_4:-76975269 None:intergenic
GCATTTCATTATGTTCTCTT+GGG 0.369117 3_4:-76974509 None:intergenic
GCGGTTACAGTCTATGCTTC+AGG 0.370432 3_4:-76975323 None:intergenic
TCTTCTTTGGGCCATAACTT+AGG 0.375370 3_4:-76974948 None:intergenic
AGCATTTCATTATGTTCTCT+TGG 0.376189 3_4:-76974510 None:intergenic
GAACATAATGAAATGCTAAC+TGG 0.379487 3_4:+76974516 Msa0523570:five_prime_UTR
GAAGTTGATGGGTCTCTCTT+AGG 0.380142 3_4:-76974924 None:intergenic
ACAGGTCCTTCTCCTAGTTA+TGG 0.385174 3_4:+76975551 Msa0523570:three_prime_UTR
CTCAGGAATTAGAAAATTGT+AGG 0.405506 3_4:+76975621 Msa0523570:three_prime_UTR
TCATCTACTAGTAATCCTTC+TGG 0.409210 3_4:+76974652 Msa0523570:CDS
TTTCAACCAATTCCCTAGCT+TGG 0.420546 3_4:-76974770 None:intergenic
GAGAAGGACCTGTTGAATCC+TGG 0.423461 3_4:-76975541 None:intergenic
CATAACTTAGGGGAAGTTGA+TGG 0.430077 3_4:-76974936 None:intergenic
ACTTTGAAAGATTTGGAAGT+AGG 0.431659 3_4:+76974879 Msa0523570:CDS
TAAGGTGCTTTCCATTGCTA+TGG 0.435162 3_4:-76975051 None:intergenic
ACCGCACTTCAAATAATAAC+CGG 0.444954 3_4:+76975349 Msa0523570:three_prime_UTR
CAAATTCCCCAGGATTCAAC+AGG 0.447810 3_4:+76975533 Msa0523570:three_prime_UTR
GAGAGTTGGTTTGATGAGAA+AGG 0.452149 3_4:-76974984 None:intergenic
GACTCATATGATTATGATGA+TGG 0.452194 3_4:-76974824 None:intergenic
TGGAGACCAAGCTAGGGAAT+TGG 0.452353 3_4:+76974764 Msa0523570:CDS
CTTTCCATTGCTATGGCTTG+TGG 0.462619 3_4:-76975044 None:intergenic
ATCAGGCAGATGTTTATCTC+AGG 0.469833 3_4:+76975604 Msa0523570:three_prime_UTR
CATCAACTTCCCCTAAGTTA+TGG 0.470154 3_4:+76974937 Msa0523570:CDS
TAATATTGATCCTTCACTAA+TGG 0.474162 3_4:+76974848 Msa0523570:CDS
GACAGTCCTCGTGACGCCTC+AGG 0.475641 3_4:-76975483 None:intergenic
AGAAGGACCTGTTGAATCCT+GGG 0.481970 3_4:-76975540 None:intergenic
TTTCTCATGGAGACCAAGCT+AGG 0.482788 3_4:+76974757 Msa0523570:CDS
GGCGTCACGAGGACTGTCCT+TGG 0.488507 3_4:+76975488 Msa0523570:three_prime_UTR
GCAAGAATGATAATTTGATC+AGG 0.489245 3_4:+76975587 Msa0523570:three_prime_UTR
GGAAGAGAACAAGATTGAAA+AGG 0.490541 3_4:-76974472 None:intergenic
AACTCTTCACAGTTATCGCT+TGG 0.503554 3_4:-76974575 None:intergenic
CAACTTTGAAAGTAGAAACA+TGG 0.510079 3_4:+76974598 Msa0523570:exon
AACTTAACCTCTCCTTTGAT+GGG 0.514268 3_4:-76975092 None:intergenic
CACTATAGTAGAAATTATGA+TGG 0.530352 3_4:+76975235 Msa0523570:CDS
AAATTCTTCTCTTTCTCTCG+AGG 0.539084 3_4:+76975017 Msa0523570:CDS
GTTCTGTAACCTCCATAACT+AGG 0.543786 3_4:-76975563 None:intergenic
TTCAAGCATAGATTCTAAAG+AGG 0.545516 3_4:-76975123 None:intergenic
TTATTTGAAGTGCGGTGTTG+CGG 0.551600 3_4:-76975342 None:intergenic
GCCGGTTATTATTTGAAGTG+CGG 0.561847 3_4:-76975350 None:intergenic
ATAACTTAGGGGAAGTTGAT+GGG 0.567226 3_4:-76974935 None:intergenic
TCAATGTGCGCAAATTCCCC+AGG 0.571882 3_4:+76975523 Msa0523570:three_prime_UTR
TTACTAGTAGATGAAGAAAG+AGG 0.580251 3_4:-76974643 None:intergenic
GTGAAGAATACCATTAGTGA+AGG 0.584434 3_4:-76974858 None:intergenic
TGCGTTGTTATTTGAAGTTG+CGG 0.585094 3_4:-76975375 None:intergenic
TGTGAAAGCAAACCCATCAA+AGG 0.587824 3_4:+76975080 Msa0523570:CDS
CAATCTTGTTCTCTTCCTCG+AGG 0.589849 3_4:+76974478 Msa0523570:five_prime_UTR
TTCTCATGGAGACCAAGCTA+GGG 0.591351 3_4:+76974758 Msa0523570:CDS
AACTTTGAAAGTAGAAACAT+GGG 0.596266 3_4:+76974599 Msa0523570:exon
GGAAATTCTAGCTCCAAAGA+TGG 0.597283 3_4:+76975256 Msa0523570:CDS
TGGGGAATTTGCGCACATTG+AGG 0.598634 3_4:-76975521 None:intergenic
TTCGTTGTTATTTGAAGTTG+CGG 0.601107 3_4:-76975402 None:intergenic
GGTCCTTCTCCTAGTTATGG+AGG 0.603331 3_4:+76975554 Msa0523570:three_prime_UTR
TTCTTTGGGCCATAACTTAG+GGG 0.604761 3_4:-76974946 None:intergenic
TAACCTCCATAACTAGGAGA+AGG 0.611853 3_4:-76975557 None:intergenic
TTCACCACAAGCCATAGCAA+TGG 0.617486 3_4:+76975040 Msa0523570:CDS
TTGGTTAGATGACTAGAATG+AGG 0.630874 3_4:-76974556 None:intergenic
GGAATTTGCGCACATTGAGG+CGG 0.637823 3_4:-76975518 None:intergenic
GAGAAGAAAACAAATTTGTG+AGG 0.639874 3_4:+76975643 Msa0523570:three_prime_UTR
ATTGAGGCGGATAAATTCCA+AGG 0.645023 3_4:-76975505 None:intergenic
GAAGGACCTGTTGAATCCTG+GGG 0.651975 3_4:-76975539 None:intergenic
GTTGTTATTTGAAGTTGCGG+TGG 0.659552 3_4:-76975399 None:intergenic
AATTCTAGCTCCAAAGATGG+TGG 0.662511 3_4:+76975259 Msa0523570:CDS
TATTAACCTGAGGCGTCACG+AGG 0.663711 3_4:+76975477 Msa0523570:three_prime_UTR
AAGCAAACCCATCAAAGGAG+AGG 0.675310 3_4:+76975085 Msa0523570:CDS
AATAAGTTAGTATTAACCTG+AGG 0.678726 3_4:+76975467 Msa0523570:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TGGGAATAAATTTTATATCT+TGG + chr3_4:76974618-76974637 Msa0523570:CDS 20.0%
!! TAAATATTTAACATACCAGA+AGG - chr3_4:76974670-76974689 None:intergenic 20.0%
!!! ACTAACTTATTCAATTTTGT+AGG - chr3_4:76975458-76975477 None:intergenic 20.0%
!!! TGGGAATAAATTTTATATCT+TGG + chr3_4:76974618-76974637 Msa0523570:CDS 20.0%
!! TAAATATTTAACATACCAGA+AGG - chr3_4:76974670-76974689 None:intergenic 20.0%
!!! ACTAACTTATTCAATTTTGT+AGG - chr3_4:76975458-76975477 None:intergenic 20.0%
!! AACTTTGAAAGTAGAAACAT+GGG + chr3_4:76974599-76974618 Msa0523570:exon 25.0%
!!! GAATTTTTTCAGTTTTCTCA+TGG + chr3_4:76974744-76974763 Msa0523570:intron 25.0%
! TAATATTGATCCTTCACTAA+TGG + chr3_4:76974848-76974867 Msa0523570:CDS 25.0%
!! ATTCTTCACTTTGAAAGATT+TGG + chr3_4:76974872-76974891 Msa0523570:CDS 25.0%
!!! ATTTTAGAAGATATGAGAGT+TGG - chr3_4:76975001-76975020 None:intergenic 25.0%
!! TTTGCTTTCACATTCTTTTA+AGG - chr3_4:76975072-76975091 None:intergenic 25.0%
! GAATTTACACAAAACATTCT+TGG + chr3_4:76975143-76975162 Msa0523570:CDS 25.0%
! TTGAAAAGTAACACTTGATT+TGG - chr3_4:76975210-76975229 None:intergenic 25.0%
! CACTATAGTAGAAATTATGA+TGG + chr3_4:76975235-76975254 Msa0523570:CDS 25.0%
! AATAAGTTAGTATTAACCTG+AGG + chr3_4:76975467-76975486 Msa0523570:three_prime_UTR 25.0%
!! AACTTTGAAAGTAGAAACAT+GGG + chr3_4:76974599-76974618 Msa0523570:exon 25.0%
!!! GAATTTTTTCAGTTTTCTCA+TGG + chr3_4:76974744-76974763 Msa0523570:intron 25.0%
! TAATATTGATCCTTCACTAA+TGG + chr3_4:76974848-76974867 Msa0523570:CDS 25.0%
!! ATTCTTCACTTTGAAAGATT+TGG + chr3_4:76974872-76974891 Msa0523570:CDS 25.0%
!!! ATTTTAGAAGATATGAGAGT+TGG - chr3_4:76975001-76975020 None:intergenic 25.0%
!! TTTGCTTTCACATTCTTTTA+AGG - chr3_4:76975072-76975091 None:intergenic 25.0%
! GAATTTACACAAAACATTCT+TGG + chr3_4:76975143-76975162 Msa0523570:CDS 25.0%
! TTGAAAAGTAACACTTGATT+TGG - chr3_4:76975210-76975229 None:intergenic 25.0%
! CACTATAGTAGAAATTATGA+TGG + chr3_4:76975235-76975254 Msa0523570:CDS 25.0%
! AATAAGTTAGTATTAACCTG+AGG + chr3_4:76975467-76975486 Msa0523570:three_prime_UTR 25.0%
GCATTTCATTATGTTCTCTT+GGG - chr3_4:76974512-76974531 None:intergenic 30.0%
AGCATTTCATTATGTTCTCT+TGG - chr3_4:76974513-76974532 None:intergenic 30.0%
GAACATAATGAAATGCTAAC+TGG + chr3_4:76974516-76974535 Msa0523570:five_prime_UTR 30.0%
! CAACTTTGAAAGTAGAAACA+TGG + chr3_4:76974598-76974617 Msa0523570:exon 30.0%
TTACTAGTAGATGAAGAAAG+AGG - chr3_4:76974646-76974665 None:intergenic 30.0%
GACTCATATGATTATGATGA+TGG - chr3_4:76974827-76974846 None:intergenic 30.0%
! ACTTTGAAAGATTTGGAAGT+AGG + chr3_4:76974879-76974898 Msa0523570:CDS 30.0%
! TTCAAGCATAGATTCTAAAG+AGG - chr3_4:76975126-76975145 None:intergenic 30.0%
ACAAAAACAGCATAAGGATA+AGG - chr3_4:76975299-76975318 None:intergenic 30.0%
!! TTCGTTGTTATTTGAAGTTG+CGG - chr3_4:76975405-76975424 None:intergenic 30.0%
!!! TCTATTTGTTTTGCAGAATC+TGG - chr3_4:76975428-76975447 None:intergenic 30.0%
GCAAGAATGATAATTTGATC+AGG + chr3_4:76975587-76975606 Msa0523570:three_prime_UTR 30.0%
CTCAGGAATTAGAAAATTGT+AGG + chr3_4:76975621-76975640 Msa0523570:three_prime_UTR 30.0%
GAGAAGAAAACAAATTTGTG+AGG + chr3_4:76975643-76975662 Msa0523570:three_prime_UTR 30.0%
GCATTTCATTATGTTCTCTT+GGG - chr3_4:76974512-76974531 None:intergenic 30.0%
AGCATTTCATTATGTTCTCT+TGG - chr3_4:76974513-76974532 None:intergenic 30.0%
GAACATAATGAAATGCTAAC+TGG + chr3_4:76974516-76974535 Msa0523570:five_prime_UTR 30.0%
! CAACTTTGAAAGTAGAAACA+TGG + chr3_4:76974598-76974617 Msa0523570:exon 30.0%
TTACTAGTAGATGAAGAAAG+AGG - chr3_4:76974646-76974665 None:intergenic 30.0%
GACTCATATGATTATGATGA+TGG - chr3_4:76974827-76974846 None:intergenic 30.0%
! ACTTTGAAAGATTTGGAAGT+AGG + chr3_4:76974879-76974898 Msa0523570:CDS 30.0%
! TTCAAGCATAGATTCTAAAG+AGG - chr3_4:76975126-76975145 None:intergenic 30.0%
ACAAAAACAGCATAAGGATA+AGG - chr3_4:76975299-76975318 None:intergenic 30.0%
!! TTCGTTGTTATTTGAAGTTG+CGG - chr3_4:76975405-76975424 None:intergenic 30.0%
!!! TCTATTTGTTTTGCAGAATC+TGG - chr3_4:76975428-76975447 None:intergenic 30.0%
GCAAGAATGATAATTTGATC+AGG + chr3_4:76975587-76975606 Msa0523570:three_prime_UTR 30.0%
CTCAGGAATTAGAAAATTGT+AGG + chr3_4:76975621-76975640 Msa0523570:three_prime_UTR 30.0%
GGAAGAGAACAAGATTGAAA+AGG - chr3_4:76974475-76974494 None:intergenic 35.0%
TTGGTTAGATGACTAGAATG+AGG - chr3_4:76974559-76974578 None:intergenic 35.0%
TCATCTACTAGTAATCCTTC+TGG + chr3_4:76974652-76974671 Msa0523570:CDS 35.0%
GTGAAGAATACCATTAGTGA+AGG - chr3_4:76974861-76974880 None:intergenic 35.0%
! ATAACTTAGGGGAAGTTGAT+GGG - chr3_4:76974938-76974957 None:intergenic 35.0%
AGTGATTCAGCTTCTTCTTT+GGG - chr3_4:76974963-76974982 None:intergenic 35.0%
AAGTGATTCAGCTTCTTCTT+TGG - chr3_4:76974964-76974983 None:intergenic 35.0%
AAATTCTTCTCTTTCTCTCG+AGG + chr3_4:76975017-76975036 Msa0523570:CDS 35.0%
AACTTAACCTCTCCTTTGAT+GGG - chr3_4:76975095-76975114 None:intergenic 35.0%
GGTACAACAAAAACAGCATA+AGG - chr3_4:76975305-76975324 None:intergenic 35.0%
ACCGCACTTCAAATAATAAC+CGG + chr3_4:76975349-76975368 Msa0523570:three_prime_UTR 35.0%
!! TGCGTTGTTATTTGAAGTTG+CGG - chr3_4:76975378-76975397 None:intergenic 35.0%
GGAAGAGAACAAGATTGAAA+AGG - chr3_4:76974475-76974494 None:intergenic 35.0%
TTGGTTAGATGACTAGAATG+AGG - chr3_4:76974559-76974578 None:intergenic 35.0%
TCATCTACTAGTAATCCTTC+TGG + chr3_4:76974652-76974671 Msa0523570:CDS 35.0%
GTGAAGAATACCATTAGTGA+AGG - chr3_4:76974861-76974880 None:intergenic 35.0%
! ATAACTTAGGGGAAGTTGAT+GGG - chr3_4:76974938-76974957 None:intergenic 35.0%
AGTGATTCAGCTTCTTCTTT+GGG - chr3_4:76974963-76974982 None:intergenic 35.0%
AAGTGATTCAGCTTCTTCTT+TGG - chr3_4:76974964-76974983 None:intergenic 35.0%
AAATTCTTCTCTTTCTCTCG+AGG + chr3_4:76975017-76975036 Msa0523570:CDS 35.0%
AACTTAACCTCTCCTTTGAT+GGG - chr3_4:76975095-76975114 None:intergenic 35.0%
GGTACAACAAAAACAGCATA+AGG - chr3_4:76975305-76975324 None:intergenic 35.0%
ACCGCACTTCAAATAATAAC+CGG + chr3_4:76975349-76975368 Msa0523570:three_prime_UTR 35.0%
!! TGCGTTGTTATTTGAAGTTG+CGG - chr3_4:76975378-76975397 None:intergenic 35.0%
AACTCTTCACAGTTATCGCT+TGG - chr3_4:76974578-76974597 None:intergenic 40.0%
TTTCAACCAATTCCCTAGCT+TGG - chr3_4:76974773-76974792 None:intergenic 40.0%
CATAACTTAGGGGAAGTTGA+TGG - chr3_4:76974939-76974958 None:intergenic 40.0%
CATCAACTTCCCCTAAGTTA+TGG + chr3_4:76974937-76974956 Msa0523570:CDS 40.0%
TTCTTTGGGCCATAACTTAG+GGG - chr3_4:76974949-76974968 None:intergenic 40.0%
CTTCTTTGGGCCATAACTTA+GGG - chr3_4:76974950-76974969 None:intergenic 40.0%
TCTTCTTTGGGCCATAACTT+AGG - chr3_4:76974951-76974970 None:intergenic 40.0%
!! GAGAGTTGGTTTGATGAGAA+AGG - chr3_4:76974987-76975006 None:intergenic 40.0%
! TAAGGTGCTTTCCATTGCTA+TGG - chr3_4:76975054-76975073 None:intergenic 40.0%
TGTGAAAGCAAACCCATCAA+AGG + chr3_4:76975080-76975099 Msa0523570:CDS 40.0%
GAACTTAACCTCTCCTTTGA+TGG - chr3_4:76975096-76975115 None:intergenic 40.0%
!! GGAAATTCTAGCTCCAAAGA+TGG + chr3_4:76975256-76975275 Msa0523570:CDS 40.0%
!! AATTCTAGCTCCAAAGATGG+TGG + chr3_4:76975259-76975278 Msa0523570:CDS 40.0%
! TTATTTGAAGTGCGGTGTTG+CGG - chr3_4:76975345-76975364 None:intergenic 40.0%
! GCCGGTTATTATTTGAAGTG+CGG - chr3_4:76975353-76975372 None:intergenic 40.0%
! TTATTTGAAGTTGCGGTTGC+CGG - chr3_4:76975371-76975390 None:intergenic 40.0%
!! GTTGTTATTTGAAGTTGCGG+TGG - chr3_4:76975375-76975394 None:intergenic 40.0%
ATTGAGGCGGATAAATTCCA+AGG - chr3_4:76975508-76975527 None:intergenic 40.0%
TAACCTCCATAACTAGGAGA+AGG - chr3_4:76975560-76975579 None:intergenic 40.0%
GTTCTGTAACCTCCATAACT+AGG - chr3_4:76975566-76975585 None:intergenic 40.0%
ATCAGGCAGATGTTTATCTC+AGG + chr3_4:76975604-76975623 Msa0523570:three_prime_UTR 40.0%
AACTCTTCACAGTTATCGCT+TGG - chr3_4:76974578-76974597 None:intergenic 40.0%
TTTCAACCAATTCCCTAGCT+TGG - chr3_4:76974773-76974792 None:intergenic 40.0%
CATAACTTAGGGGAAGTTGA+TGG - chr3_4:76974939-76974958 None:intergenic 40.0%
CATCAACTTCCCCTAAGTTA+TGG + chr3_4:76974937-76974956 Msa0523570:CDS 40.0%
TTCTTTGGGCCATAACTTAG+GGG - chr3_4:76974949-76974968 None:intergenic 40.0%
CTTCTTTGGGCCATAACTTA+GGG - chr3_4:76974950-76974969 None:intergenic 40.0%
TCTTCTTTGGGCCATAACTT+AGG - chr3_4:76974951-76974970 None:intergenic 40.0%
!! GAGAGTTGGTTTGATGAGAA+AGG - chr3_4:76974987-76975006 None:intergenic 40.0%
! TAAGGTGCTTTCCATTGCTA+TGG - chr3_4:76975054-76975073 None:intergenic 40.0%
TGTGAAAGCAAACCCATCAA+AGG + chr3_4:76975080-76975099 Msa0523570:CDS 40.0%
GAACTTAACCTCTCCTTTGA+TGG - chr3_4:76975096-76975115 None:intergenic 40.0%
!! GGAAATTCTAGCTCCAAAGA+TGG + chr3_4:76975256-76975275 Msa0523570:CDS 40.0%
!! AATTCTAGCTCCAAAGATGG+TGG + chr3_4:76975259-76975278 Msa0523570:CDS 40.0%
! TTATTTGAAGTGCGGTGTTG+CGG - chr3_4:76975345-76975364 None:intergenic 40.0%
! GCCGGTTATTATTTGAAGTG+CGG - chr3_4:76975353-76975372 None:intergenic 40.0%
! TTATTTGAAGTTGCGGTTGC+CGG - chr3_4:76975371-76975390 None:intergenic 40.0%
!! GTTGTTATTTGAAGTTGCGG+TGG - chr3_4:76975375-76975394 None:intergenic 40.0%
ATTGAGGCGGATAAATTCCA+AGG - chr3_4:76975508-76975527 None:intergenic 40.0%
TAACCTCCATAACTAGGAGA+AGG - chr3_4:76975560-76975579 None:intergenic 40.0%
GTTCTGTAACCTCCATAACT+AGG - chr3_4:76975566-76975585 None:intergenic 40.0%
ATCAGGCAGATGTTTATCTC+AGG + chr3_4:76975604-76975623 Msa0523570:three_prime_UTR 40.0%
CAATCTTGTTCTCTTCCTCG+AGG + chr3_4:76974478-76974497 Msa0523570:five_prime_UTR 45.0%
TTTCTCATGGAGACCAAGCT+AGG + chr3_4:76974757-76974776 Msa0523570:CDS 45.0%
TTCTCATGGAGACCAAGCTA+GGG + chr3_4:76974758-76974777 Msa0523570:CDS 45.0%
!! GAAGTTGATGGGTCTCTCTT+AGG - chr3_4:76974927-76974946 None:intergenic 45.0%
TTCACCACAAGCCATAGCAA+TGG + chr3_4:76975040-76975059 Msa0523570:CDS 45.0%
CTTTCCATTGCTATGGCTTG+TGG - chr3_4:76975047-76975066 None:intergenic 45.0%
AAGCAAACCCATCAAAGGAG+AGG + chr3_4:76975085-76975104 Msa0523570:CDS 45.0%
CAAATTCCCCAGGATTCAAC+AGG + chr3_4:76975533-76975552 Msa0523570:three_prime_UTR 45.0%
AGAAGGACCTGTTGAATCCT+GGG - chr3_4:76975543-76975562 None:intergenic 45.0%
ACAGGTCCTTCTCCTAGTTA+TGG + chr3_4:76975551-76975570 Msa0523570:three_prime_UTR 45.0%
CAATCTTGTTCTCTTCCTCG+AGG + chr3_4:76974478-76974497 Msa0523570:five_prime_UTR 45.0%
TTTCTCATGGAGACCAAGCT+AGG + chr3_4:76974757-76974776 Msa0523570:CDS 45.0%
TTCTCATGGAGACCAAGCTA+GGG + chr3_4:76974758-76974777 Msa0523570:CDS 45.0%
!! GAAGTTGATGGGTCTCTCTT+AGG - chr3_4:76974927-76974946 None:intergenic 45.0%
TTCACCACAAGCCATAGCAA+TGG + chr3_4:76975040-76975059 Msa0523570:CDS 45.0%
CTTTCCATTGCTATGGCTTG+TGG - chr3_4:76975047-76975066 None:intergenic 45.0%
AAGCAAACCCATCAAAGGAG+AGG + chr3_4:76975085-76975104 Msa0523570:CDS 45.0%
CAAATTCCCCAGGATTCAAC+AGG + chr3_4:76975533-76975552 Msa0523570:three_prime_UTR 45.0%
AGAAGGACCTGTTGAATCCT+GGG - chr3_4:76975543-76975562 None:intergenic 45.0%
ACAGGTCCTTCTCCTAGTTA+TGG + chr3_4:76975551-76975570 Msa0523570:three_prime_UTR 45.0%
!!! GATTATTATAAAATTTTATT+TGG - chr3_4:76974431-76974450 None:intergenic 5.0%
!!! GATTATTATAAAATTTTATT+TGG - chr3_4:76974431-76974450 None:intergenic 5.0%
! TCTTGGGCACATTTTCCTCG+AGG - chr3_4:76974496-76974515 None:intergenic 50.0%
TGGAGACCAAGCTAGGGAAT+TGG + chr3_4:76974764-76974783 Msa0523570:CDS 50.0%
GTGTGACAAGCCACCATCTT+TGG - chr3_4:76975272-76975291 None:intergenic 50.0%
GCGGTTACAGTCTATGCTTC+AGG - chr3_4:76975326-76975345 None:intergenic 50.0%
! TATTAACCTGAGGCGTCACG+AGG + chr3_4:76975477-76975496 Msa0523570:three_prime_UTR 50.0%
GGAATTTGCGCACATTGAGG+CGG - chr3_4:76975521-76975540 None:intergenic 50.0%
TGGGGAATTTGCGCACATTG+AGG - chr3_4:76975524-76975543 None:intergenic 50.0%
TCAATGTGCGCAAATTCCCC+AGG + chr3_4:76975523-76975542 Msa0523570:three_prime_UTR 50.0%
GAAGGACCTGTTGAATCCTG+GGG - chr3_4:76975542-76975561 None:intergenic 50.0%
GAGAAGGACCTGTTGAATCC+TGG - chr3_4:76975544-76975563 None:intergenic 50.0%
GGTCCTTCTCCTAGTTATGG+AGG + chr3_4:76975554-76975573 Msa0523570:three_prime_UTR 50.0%
! TCTTGGGCACATTTTCCTCG+AGG - chr3_4:76974496-76974515 None:intergenic 50.0%
TGGAGACCAAGCTAGGGAAT+TGG + chr3_4:76974764-76974783 Msa0523570:CDS 50.0%
GTGTGACAAGCCACCATCTT+TGG - chr3_4:76975272-76975291 None:intergenic 50.0%
GCGGTTACAGTCTATGCTTC+AGG - chr3_4:76975326-76975345 None:intergenic 50.0%
! TATTAACCTGAGGCGTCACG+AGG + chr3_4:76975477-76975496 Msa0523570:three_prime_UTR 50.0%
GGAATTTGCGCACATTGAGG+CGG - chr3_4:76975521-76975540 None:intergenic 50.0%
TGGGGAATTTGCGCACATTG+AGG - chr3_4:76975524-76975543 None:intergenic 50.0%
TCAATGTGCGCAAATTCCCC+AGG + chr3_4:76975523-76975542 Msa0523570:three_prime_UTR 50.0%
GAAGGACCTGTTGAATCCTG+GGG - chr3_4:76975542-76975561 None:intergenic 50.0%
GAGAAGGACCTGTTGAATCC+TGG - chr3_4:76975544-76975563 None:intergenic 50.0%
GGTCCTTCTCCTAGTTATGG+AGG + chr3_4:76975554-76975573 Msa0523570:three_prime_UTR 50.0%
GACAGTCCTCGTGACGCCTC+AGG - chr3_4:76975486-76975505 None:intergenic 65.0%
GGCGTCACGAGGACTGTCCT+TGG + chr3_4:76975488-76975507 Msa0523570:three_prime_UTR 65.0%
GACAGTCCTCGTGACGCCTC+AGG - chr3_4:76975486-76975505 None:intergenic 65.0%
GGCGTCACGAGGACTGTCCT+TGG + chr3_4:76975488-76975507 Msa0523570:three_prime_UTR 65.0%
Chromosome Type Strat End Strand Name
chr3_4 gene 76974405 76975665 76974405 ID=Msa0523570;Name=Msa0523570
chr3_4 mRNA 76974405 76975665 76974405 ID=Msa0523570-mRNA-1;Parent=Msa0523570;Name=Msa0523570-mRNA-1;_AED=0.10;_eAED=0.10;_QI=212|1|1|1|1|1|2|331|211
chr3_4 exon 76974405 76974673 76974405 ID=Msa0523570-mRNA-1:exon:22441;Parent=Msa0523570-mRNA-1
chr3_4 exon 76974756 76975665 76974756 ID=Msa0523570-mRNA-1:exon:22442;Parent=Msa0523570-mRNA-1
chr3_4 five_prime_UTR 76974405 76974616 76974405 ID=Msa0523570-mRNA-1:five_prime_utr;Parent=Msa0523570-mRNA-1
chr3_4 CDS 76974617 76974673 76974617 ID=Msa0523570-mRNA-1:cds;Parent=Msa0523570-mRNA-1
chr3_4 CDS 76974756 76975334 76974756 ID=Msa0523570-mRNA-1:cds;Parent=Msa0523570-mRNA-1
chr3_4 three_prime_UTR 76975335 76975665 76975335 ID=Msa0523570-mRNA-1:three_prime_utr;Parent=Msa0523570-mRNA-1
Gene Sequence

>Msa0523570

ATGGGAATAAATTTTATATCTTGGATCCTCTTTCTTCATCTACTAGTAATCCTTCTGTTTTCTCATGGAGACCAAGCTAGGGAATTGGTTGAAACAGAAGACAAATCATTGAAAATTCAACACAACCATCATCATAATCATATGAGTCATAATATTGATCCTTCACTAATGGTATTCTTCACTTTGAAAGATTTGGAAGTAGGAAAAATAATGCAAATTTATTTTCCTAAGAGAGACCCATCAACTTCCCCTAAGTTATGGCCCAAAGAAGAAGCTGAATCACTTCCTTTCTCATCAAACCAACTCTCATATCTTCTAAAATTCTTCTCTTTCTCTCGAGGTTCACCACAAGCCATAGCAATGGAAAGCACCTTAAAAGAATGTGAAAGCAAACCCATCAAAGGAGAGGTTAAGTTCTGTGCTACCTCTTTAGAATCTATGCTTGAATTTACACAAAACATTCTTGGTTCAGAATCTGATATTCAAGCTTATGCAACTTTGCACAAAACCAAATCAAGTGTTACTTTTCAAAACTACACTATAGTAGAAATTATGATGGAAATTCTAGCTCCAAAGATGGTGGCTTGTCACACTGTGCCTTATCCTTATGCTGTTTTTGTTGTACCTGAAGCATAG
Protein sequence

>Msa0523570

MGINFISWILFLHLLVILLFSHGDQARELVETEDKSLKIQHNHHHNHMSHNIDPSLMVFFTLKDLEVGKIMQIYFPKRDPSTSPKLWPKEEAESLPFSSNQLSYLLKFFSFSRGSPQAIAMESTLKECESKPIKGEVKFCATSLESMLEFTQNILGSESDIQAYATLHKTKSSVTFQNYTIVEIMMEILAPKMVACHTVPYPYAVFVVPEA*