AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0594120

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0594120 MtrunA17_Chr4g0012811 99.541 218 1 0 1 218 1 218 2.01e-162 447
Msa0594120 MtrunA17_Chr7g0231431 83.111 225 30 3 1 218 1 224 4.83e-134 375
Msa0594120 MtrunA17_Chr7g0250121 46.541 159 79 2 29 187 11 163 8.07e-46 150
Msa0594120 MtrunA17_Chr8g0335971 45.732 164 81 1 24 187 5 160 1.02e-45 150
Msa0594120 MtrunA17_Chr7g0259831 47.541 122 63 1 65 186 63 183 3.00e-37 129
Msa0594120 MtrunA17_Chr2g0322921 43.169 183 93 3 27 199 24 205 1.40e-33 120
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0594120 AT2G45080.1 72.768 224 47 3 5 218 3 222 1.08e-114 327
Msa0594120 AT3G60550.1 70.044 227 57 1 3 218 4 230 4.25e-113 323
Msa0594120 AT2G44740.1 44.654 159 84 2 31 189 13 167 1.08e-43 145
Msa0594120 AT5G07450.1 40.351 171 86 2 29 193 21 181 6.87e-41 139
Msa0594120 AT5G61650.1 40.373 161 88 1 29 189 23 175 6.25e-39 134
Msa0594120 AT3G63120.2 42.958 142 78 2 59 200 58 196 2.79e-38 132
Msa0594120 AT3G63120.1 42.958 142 78 2 59 200 58 196 2.79e-38 132
Msa0594120 AT3G21870.1 39.394 165 97 2 25 189 21 182 1.15e-35 125
Msa0594120 AT3G05327.1 37.725 167 97 2 25 187 27 190 5.91e-33 118

Find 49 sgRNAs with CRISPR-Local

Find 228 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGATAATTATTTGGACTATT+TGG 0.122443 4_2:-21183321 None:intergenic
TTCTAAACCCAAGATTATTT+TGG 0.173257 4_2:-21183843 None:intergenic
TTTGTTGTTAAGAAATTATT+TGG 0.237058 4_2:-21183414 None:intergenic
GAGGGAGAAAGATAATTATT+TGG 0.256810 4_2:-21183330 None:intergenic
ACTTCTCATAACAACTATTA+TGG 0.285093 4_2:+21183888 Msa0594120:CDS
TGTGATGGAACAATACAAAT+TGG 0.286229 4_2:-21185155 None:intergenic
GCTAGAACATTTATCACTAA+TGG 0.307122 4_2:-21183613 None:intergenic
GGAGTGGAATTTATATATAA+AGG 0.322270 4_2:-21183371 None:intergenic
TGAATCTTCTTTGTATGAAT+AGG 0.344032 4_2:-21183584 None:intergenic
CTAGAACATTTATCACTAAT+GGG 0.347684 4_2:-21183612 None:intergenic
TGAGAGCTATTGTTCCCATT+TGG 0.387926 4_2:+21184674 Msa0594120:CDS
AATTCATACTTTGCAAAAGT+TGG 0.389350 4_2:+21184564 Msa0594120:CDS
GATTTCTTCAGCACACCTTA+AGG 0.402870 4_2:-21184739 None:intergenic
TACTCACACTCAATAATATA+CGG 0.406412 4_2:-21184866 None:intergenic
TGCTTACTTCCCTCTCCAAA+TGG 0.411186 4_2:-21184689 None:intergenic
TAAGATTGAATGGTTAAATC+AGG 0.411388 4_2:-21183742 None:intergenic
TCTCTCTAAATAAGATTGAA+TGG 0.421099 4_2:-21183752 None:intergenic
GAGAGGGAAGTAAGCATTGG+AGG 0.422508 4_2:+21184696 Msa0594120:CDS
AATTCATTGTTTCTTTGTGA+TGG 0.426845 4_2:-21185170 None:intergenic
ATACACACGTGCTGGACCTT+CGG 0.437594 4_2:+21183783 Msa0594120:CDS
TAAATTTAAATGTTTGTTGA+GGG 0.442331 4_2:+21185086 Msa0594120:three_prime_UTR
AGGGAAGTAAGCATTGGAGG+AGG 0.444484 4_2:+21184699 Msa0594120:CDS
TTGAGTGTGAGTATCAATCT+TGG 0.464497 4_2:+21184876 Msa0594120:three_prime_UTR
TTGCTTATGTCTATATTGAT+CGG 0.466190 4_2:+21183815 Msa0594120:CDS
AAGGAGAATGGAGTTGTCAA+TGG 0.491097 4_2:-21183352 None:intergenic
TCATACTTTGCAAAAGTTGG+TGG 0.491361 4_2:+21184567 Msa0594120:CDS
TGAGTGTGAGTATCAATCTT+GGG 0.498750 4_2:+21184877 Msa0594120:three_prime_UTR
AACAAATGAATTGAATGAGT+TGG 0.500361 4_2:+21184596 Msa0594120:CDS
ATTGGTACATCGCTTTCAGC+TGG 0.502137 4_2:-21185137 None:intergenic
ACCACATTGAGAGGACCTTA+AGG 0.502225 4_2:+21184724 Msa0594120:CDS
GTAAATTTAAATGTTTGTTG+AGG 0.514689 4_2:+21185085 Msa0594120:three_prime_UTR
TGGACTATTTGGATTGAGAG+AGG 0.531526 4_2:-21183310 None:intergenic
GCTGAAGAAATCAAAGAAAG+AGG 0.532244 4_2:+21184750 Msa0594120:CDS
TTGGAGAGGGAAGTAAGCAT+TGG 0.532407 4_2:+21184693 Msa0594120:CDS
GCTTACTTCCCTCTCCAAAT+GGG 0.534304 4_2:-21184688 None:intergenic
AGAATGGAGTTGTCAATGGA+GGG 0.536884 4_2:-21183348 None:intergenic
AATTTATATATAAAGGAGAA+TGG 0.542353 4_2:-21183364 None:intergenic
GGACTATTTGGATTGAGAGA+GGG 0.553934 4_2:-21183309 None:intergenic
GAGAATGGAGTTGTCAATGG+AGG 0.555049 4_2:-21183349 None:intergenic
AAATTTAAATGTTTGTTGAG+GGG 0.590012 4_2:+21185087 Msa0594120:three_prime_UTR
GGGAAGTAAGCATTGGAGGA+GGG 0.593739 4_2:+21184700 Msa0594120:CDS
CTATTGTTCCCATTTGGAGA+GGG 0.594811 4_2:+21184680 Msa0594120:CDS
GCTATTGTTCCCATTTGGAG+AGG 0.621485 4_2:+21184679 Msa0594120:CDS
GAGGAGGGTACCACATTGAG+AGG 0.624143 4_2:+21184715 Msa0594120:CDS
ATCTTGGGAGTGACAACTCG+AGG 0.627964 4_2:+21184892 Msa0594120:three_prime_UTR
TTCATTAATTGAGAGAAACA+TGG 0.648460 4_2:+21183636 Msa0594120:CDS
TAGAACATTTATCACTAATG+GGG 0.651890 4_2:-21183611 None:intergenic
ACCTTAAGGTCCTCTCAATG+TGG 0.678220 4_2:-21184725 None:intergenic
TAAGCAACAACATAAACCGA+AGG 0.681604 4_2:-21183799 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTAATTTAACAACAAATA+TGG + chr4_2:21184397-21184416 Msa0594120:intron 10.0%
!!! AAAAAAATTCTCTTTATTTT+AGG + chr4_2:21185011-21185030 Msa0594120:three_prime_UTR 10.0%
!! AATTAATTTAACAACAAATA+TGG + chr4_2:21184397-21184416 Msa0594120:intron 10.0%
!!! AAAAAAATTCTCTTTATTTT+AGG + chr4_2:21185011-21185030 Msa0594120:three_prime_UTR 10.0%
!! AATTTATATATAAAGGAGAA+TGG - chr4_2:21183367-21183386 None:intergenic 15.0%
!!! TTTGTTGTTAAGAAATTATT+TGG - chr4_2:21183417-21183436 None:intergenic 15.0%
!!! AATTTCTTACATTATTTTCA+AGG + chr4_2:21184006-21184025 Msa0594120:intron 15.0%
!! TTGTTTATTAAAATCTCAAT+CGG + chr4_2:21184068-21184087 Msa0594120:intron 15.0%
!! ATTAATGGTCAAATTTAATT+TGG + chr4_2:21184252-21184271 Msa0594120:intron 15.0%
!!! ATAAACATCTTGAATTTTAA+TGG - chr4_2:21184345-21184364 None:intergenic 15.0%
!! TTAATTCAACTATAAACATT+TGG - chr4_2:21184383-21184402 None:intergenic 15.0%
!!! TTTCTATATTTAGTTTATTG+TGG + chr4_2:21184492-21184511 Msa0594120:intron 15.0%
!! AAAAAAAAAAAAAAACTCGA+GGG + chr4_2:21184928-21184947 Msa0594120:three_prime_UTR 15.0%
!!! TTTTTTTTCTTGCAAAATAA+AGG + chr4_2:21185036-21185055 Msa0594120:three_prime_UTR 15.0%
!!! TAAATTTAAATGTTTGTTGA+GGG + chr4_2:21185086-21185105 Msa0594120:three_prime_UTR 15.0%
!! AATTTATATATAAAGGAGAA+TGG - chr4_2:21183367-21183386 None:intergenic 15.0%
!!! TTTGTTGTTAAGAAATTATT+TGG - chr4_2:21183417-21183436 None:intergenic 15.0%
!!! AATTTCTTACATTATTTTCA+AGG + chr4_2:21184006-21184025 Msa0594120:intron 15.0%
!! TTGTTTATTAAAATCTCAAT+CGG + chr4_2:21184068-21184087 Msa0594120:intron 15.0%
!! ATTAATGGTCAAATTTAATT+TGG + chr4_2:21184252-21184271 Msa0594120:intron 15.0%
!!! ATAAACATCTTGAATTTTAA+TGG - chr4_2:21184345-21184364 None:intergenic 15.0%
!! TTAATTCAACTATAAACATT+TGG - chr4_2:21184383-21184402 None:intergenic 15.0%
!!! TTTCTATATTTAGTTTATTG+TGG + chr4_2:21184492-21184511 Msa0594120:intron 15.0%
!! AAAAAAAAAAAAAAACTCGA+GGG + chr4_2:21184928-21184947 Msa0594120:three_prime_UTR 15.0%
!!! TTTTTTTTCTTGCAAAATAA+AGG + chr4_2:21185036-21185055 Msa0594120:three_prime_UTR 15.0%
!!! TAAATTTAAATGTTTGTTGA+GGG + chr4_2:21185086-21185105 Msa0594120:three_prime_UTR 15.0%
!! AGATAATTATTTGGACTATT+TGG - chr4_2:21183324-21183343 None:intergenic 20.0%
!! AAAACAAATAGAGAAAAAGA+AGG - chr4_2:21183956-21183975 None:intergenic 20.0%
!!! TTATTTTCAAGGTAACAATT+TGG + chr4_2:21184017-21184036 Msa0594120:intron 20.0%
!!! AATGTATCTCTTTTAATATG+AGG - chr4_2:21184190-21184209 None:intergenic 20.0%
!!! TTTTTTTTTTTGAGAGATCT+CGG - chr4_2:21184919-21184938 None:intergenic 20.0%
!! CAAAAAAAAAAAAAAACTCG+AGG + chr4_2:21184927-21184946 Msa0594120:three_prime_UTR 20.0%
!!! AGTTTTAAATAGTTTTCTCT+CGG - chr4_2:21184954-21184973 None:intergenic 20.0%
!! ACTGAAAAATCATAGATATA+TGG + chr4_2:21184971-21184990 Msa0594120:three_prime_UTR 20.0%
!!! GTAAATTTAAATGTTTGTTG+AGG + chr4_2:21185085-21185104 Msa0594120:three_prime_UTR 20.0%
!!! AAATTTAAATGTTTGTTGAG+GGG + chr4_2:21185087-21185106 Msa0594120:three_prime_UTR 20.0%
!! AGATAATTATTTGGACTATT+TGG - chr4_2:21183324-21183343 None:intergenic 20.0%
!! AAAACAAATAGAGAAAAAGA+AGG - chr4_2:21183956-21183975 None:intergenic 20.0%
!!! TTATTTTCAAGGTAACAATT+TGG + chr4_2:21184017-21184036 Msa0594120:intron 20.0%
!!! AATGTATCTCTTTTAATATG+AGG - chr4_2:21184190-21184209 None:intergenic 20.0%
!!! TTTTTTTTTTTGAGAGATCT+CGG - chr4_2:21184919-21184938 None:intergenic 20.0%
!! CAAAAAAAAAAAAAAACTCG+AGG + chr4_2:21184927-21184946 Msa0594120:three_prime_UTR 20.0%
!!! AGTTTTAAATAGTTTTCTCT+CGG - chr4_2:21184954-21184973 None:intergenic 20.0%
!! ACTGAAAAATCATAGATATA+TGG + chr4_2:21184971-21184990 Msa0594120:three_prime_UTR 20.0%
!!! GTAAATTTAAATGTTTGTTG+AGG + chr4_2:21185085-21185104 Msa0594120:three_prime_UTR 20.0%
!!! AAATTTAAATGTTTGTTGAG+GGG + chr4_2:21185087-21185106 Msa0594120:three_prime_UTR 20.0%
! GGAGTGGAATTTATATATAA+AGG - chr4_2:21183374-21183393 None:intergenic 25.0%
!!! TCTTTTTTTCTTGTAGAAGA+AGG - chr4_2:21183489-21183508 None:intergenic 25.0%
! AAGAAGAATCCATCAAAAAA+GGG - chr4_2:21183522-21183541 None:intergenic 25.0%
!! AAATCATAGTGTAGTTTTCT+TGG - chr4_2:21183559-21183578 None:intergenic 25.0%
! TGAATCTTCTTTGTATGAAT+AGG - chr4_2:21183587-21183606 None:intergenic 25.0%
! TAGAACATTTATCACTAATG+GGG - chr4_2:21183614-21183633 None:intergenic 25.0%
! CTAGAACATTTATCACTAAT+GGG - chr4_2:21183615-21183634 None:intergenic 25.0%
! TTCATTAATTGAGAGAAACA+TGG + chr4_2:21183636-21183655 Msa0594120:CDS 25.0%
! TAAGATTGAATGGTTAAATC+AGG - chr4_2:21183745-21183764 None:intergenic 25.0%
! TCTCTCTAAATAAGATTGAA+TGG - chr4_2:21183755-21183774 None:intergenic 25.0%
! TTGCTTATGTCTATATTGAT+CGG + chr4_2:21183815-21183834 Msa0594120:CDS 25.0%
!! TTCTAAACCCAAGATTATTT+TGG - chr4_2:21183846-21183865 None:intergenic 25.0%
! ACTTCTCATAACAACTATTA+TGG + chr4_2:21183888-21183907 Msa0594120:CDS 25.0%
!! CTCTTTTAATATGAGGATTA+CGG - chr4_2:21184183-21184202 None:intergenic 25.0%
!!! TTTTTTTCTCTTTAATCCGA+CGG + chr4_2:21184211-21184230 Msa0594120:intron 25.0%
!!! ATTTTAATGGTAGAGAATCT+AGG - chr4_2:21184332-21184351 None:intergenic 25.0%
!!! TTTTTTGTCAAAATCTCCAA+TGG - chr4_2:21184458-21184477 None:intergenic 25.0%
! AATTCATACTTTGCAAAAGT+TGG + chr4_2:21184564-21184583 Msa0594120:CDS 25.0%
! AACAAATGAATTGAATGAGT+TGG + chr4_2:21184596-21184615 Msa0594120:CDS 25.0%
!! TGTAATGTTGTAGACTTTTT+AGG + chr4_2:21184791-21184810 Msa0594120:exon 25.0%
!! GTAATGTTGTAGACTTTTTA+GGG + chr4_2:21184792-21184811 Msa0594120:exon 25.0%
! TACTCACACTCAATAATATA+CGG - chr4_2:21184869-21184888 None:intergenic 25.0%
!! TTTTCTTGCAAAATAAAGGA+AGG + chr4_2:21185040-21185059 Msa0594120:three_prime_UTR 25.0%
!!! GATTGAGATTTTAGAACTAT+AGG + chr4_2:21185109-21185128 Msa0594120:three_prime_UTR 25.0%
!! AATTCATTGTTTCTTTGTGA+TGG - chr4_2:21185173-21185192 None:intergenic 25.0%
! GGAGTGGAATTTATATATAA+AGG - chr4_2:21183374-21183393 None:intergenic 25.0%
!!! TCTTTTTTTCTTGTAGAAGA+AGG - chr4_2:21183489-21183508 None:intergenic 25.0%
! AAGAAGAATCCATCAAAAAA+GGG - chr4_2:21183522-21183541 None:intergenic 25.0%
!! AAATCATAGTGTAGTTTTCT+TGG - chr4_2:21183559-21183578 None:intergenic 25.0%
! TGAATCTTCTTTGTATGAAT+AGG - chr4_2:21183587-21183606 None:intergenic 25.0%
! TAGAACATTTATCACTAATG+GGG - chr4_2:21183614-21183633 None:intergenic 25.0%
! CTAGAACATTTATCACTAAT+GGG - chr4_2:21183615-21183634 None:intergenic 25.0%
! TTCATTAATTGAGAGAAACA+TGG + chr4_2:21183636-21183655 Msa0594120:CDS 25.0%
! TAAGATTGAATGGTTAAATC+AGG - chr4_2:21183745-21183764 None:intergenic 25.0%
! TCTCTCTAAATAAGATTGAA+TGG - chr4_2:21183755-21183774 None:intergenic 25.0%
! TTGCTTATGTCTATATTGAT+CGG + chr4_2:21183815-21183834 Msa0594120:CDS 25.0%
!! TTCTAAACCCAAGATTATTT+TGG - chr4_2:21183846-21183865 None:intergenic 25.0%
! ACTTCTCATAACAACTATTA+TGG + chr4_2:21183888-21183907 Msa0594120:CDS 25.0%
!! CTCTTTTAATATGAGGATTA+CGG - chr4_2:21184183-21184202 None:intergenic 25.0%
!!! TTTTTTTCTCTTTAATCCGA+CGG + chr4_2:21184211-21184230 Msa0594120:intron 25.0%
!!! ATTTTAATGGTAGAGAATCT+AGG - chr4_2:21184332-21184351 None:intergenic 25.0%
!!! TTTTTTGTCAAAATCTCCAA+TGG - chr4_2:21184458-21184477 None:intergenic 25.0%
! AATTCATACTTTGCAAAAGT+TGG + chr4_2:21184564-21184583 Msa0594120:CDS 25.0%
! AACAAATGAATTGAATGAGT+TGG + chr4_2:21184596-21184615 Msa0594120:CDS 25.0%
!! TGTAATGTTGTAGACTTTTT+AGG + chr4_2:21184791-21184810 Msa0594120:exon 25.0%
!! GTAATGTTGTAGACTTTTTA+GGG + chr4_2:21184792-21184811 Msa0594120:exon 25.0%
! TACTCACACTCAATAATATA+CGG - chr4_2:21184869-21184888 None:intergenic 25.0%
!! TTTTCTTGCAAAATAAAGGA+AGG + chr4_2:21185040-21185059 Msa0594120:three_prime_UTR 25.0%
!!! GATTGAGATTTTAGAACTAT+AGG + chr4_2:21185109-21185128 Msa0594120:three_prime_UTR 25.0%
!! AATTCATTGTTTCTTTGTGA+TGG - chr4_2:21185173-21185192 None:intergenic 25.0%
GAGGGAGAAAGATAATTATT+TGG - chr4_2:21183333-21183352 None:intergenic 30.0%
!!! AATGAAGAAAGTTTTAGGAG+TGG - chr4_2:21183390-21183409 None:intergenic 30.0%
! GAAGCAATGAAGAAAGTTTT+AGG - chr4_2:21183395-21183414 None:intergenic 30.0%
!!! TGTAGAAGAAGGTTTTGAAA+GGG - chr4_2:21183478-21183497 None:intergenic 30.0%
!!! TTGTAGAAGAAGGTTTTGAA+AGG - chr4_2:21183479-21183498 None:intergenic 30.0%
!!! GAAAATTTCCCCTTTTTTGA+TGG + chr4_2:21183510-21183529 Msa0594120:exon 30.0%
AGAAGAATCCATCAAAAAAG+GGG - chr4_2:21183521-21183540 None:intergenic 30.0%
GAAGAAGAATCCATCAAAAA+AGG - chr4_2:21183523-21183542 None:intergenic 30.0%
GCTAGAACATTTATCACTAA+TGG - chr4_2:21183616-21183635 None:intergenic 30.0%
! AAAGATTTTTGTGCTTGCTT+TGG - chr4_2:21183707-21183726 None:intergenic 30.0%
! GGTTTTGCCAAAATAATCTT+GGG + chr4_2:21183836-21183855 Msa0594120:CDS 30.0%
TCAAGGTAACAATTTGGTAA+AGG + chr4_2:21184023-21184042 Msa0594120:intron 30.0%
CAAGGTAACAATTTGGTAAA+GGG + chr4_2:21184024-21184043 Msa0594120:intron 30.0%
AATTATATTGCCTCAAAGCA+TGG + chr4_2:21184092-21184111 Msa0594120:intron 30.0%
TGAAGACCAGAAAAATATTG+GGG - chr4_2:21184140-21184159 None:intergenic 30.0%
GTGAAGACCAGAAAAATATT+GGG - chr4_2:21184141-21184160 None:intergenic 30.0%
TGTGAAGACCAGAAAAATAT+TGG - chr4_2:21184142-21184161 None:intergenic 30.0%
ACGGACATTAATTGCATTAA+TGG + chr4_2:21184237-21184256 Msa0594120:intron 30.0%
!! TAACTATCATTTTGACCGAA+AGG + chr4_2:21184429-21184448 Msa0594120:intron 30.0%
!! AACTATCATTTTGACCGAAA+GGG + chr4_2:21184430-21184449 Msa0594120:intron 30.0%
!!! AGATTTTAGAACTATAGGTG+AGG + chr4_2:21185114-21185133 Msa0594120:three_prime_UTR 30.0%
! TGTGATGGAACAATACAAAT+TGG - chr4_2:21185158-21185177 None:intergenic 30.0%
! CAATGAATTTTATGAGCAAG+AGG + chr4_2:21185184-21185203 Msa0594120:three_prime_UTR 30.0%
GAGGGAGAAAGATAATTATT+TGG - chr4_2:21183333-21183352 None:intergenic 30.0%
!!! AATGAAGAAAGTTTTAGGAG+TGG - chr4_2:21183390-21183409 None:intergenic 30.0%
! GAAGCAATGAAGAAAGTTTT+AGG - chr4_2:21183395-21183414 None:intergenic 30.0%
!!! TGTAGAAGAAGGTTTTGAAA+GGG - chr4_2:21183478-21183497 None:intergenic 30.0%
!!! TTGTAGAAGAAGGTTTTGAA+AGG - chr4_2:21183479-21183498 None:intergenic 30.0%
!!! GAAAATTTCCCCTTTTTTGA+TGG + chr4_2:21183510-21183529 Msa0594120:exon 30.0%
AGAAGAATCCATCAAAAAAG+GGG - chr4_2:21183521-21183540 None:intergenic 30.0%
GAAGAAGAATCCATCAAAAA+AGG - chr4_2:21183523-21183542 None:intergenic 30.0%
GCTAGAACATTTATCACTAA+TGG - chr4_2:21183616-21183635 None:intergenic 30.0%
! AAAGATTTTTGTGCTTGCTT+TGG - chr4_2:21183707-21183726 None:intergenic 30.0%
! GGTTTTGCCAAAATAATCTT+GGG + chr4_2:21183836-21183855 Msa0594120:CDS 30.0%
TCAAGGTAACAATTTGGTAA+AGG + chr4_2:21184023-21184042 Msa0594120:intron 30.0%
CAAGGTAACAATTTGGTAAA+GGG + chr4_2:21184024-21184043 Msa0594120:intron 30.0%
AATTATATTGCCTCAAAGCA+TGG + chr4_2:21184092-21184111 Msa0594120:intron 30.0%
TGAAGACCAGAAAAATATTG+GGG - chr4_2:21184140-21184159 None:intergenic 30.0%
GTGAAGACCAGAAAAATATT+GGG - chr4_2:21184141-21184160 None:intergenic 30.0%
TGTGAAGACCAGAAAAATAT+TGG - chr4_2:21184142-21184161 None:intergenic 30.0%
ACGGACATTAATTGCATTAA+TGG + chr4_2:21184237-21184256 Msa0594120:intron 30.0%
!! TAACTATCATTTTGACCGAA+AGG + chr4_2:21184429-21184448 Msa0594120:intron 30.0%
!! AACTATCATTTTGACCGAAA+GGG + chr4_2:21184430-21184449 Msa0594120:intron 30.0%
!!! AGATTTTAGAACTATAGGTG+AGG + chr4_2:21185114-21185133 Msa0594120:three_prime_UTR 30.0%
! TGTGATGGAACAATACAAAT+TGG - chr4_2:21185158-21185177 None:intergenic 30.0%
! CAATGAATTTTATGAGCAAG+AGG + chr4_2:21185184-21185203 Msa0594120:three_prime_UTR 30.0%
!!! AAGAAGGTTTTGAAAGGGAT+TGG - chr4_2:21183473-21183492 None:intergenic 35.0%
!! ATTTTTAGATACACACGTGC+TGG + chr4_2:21183775-21183794 Msa0594120:CDS 35.0%
TAAGCAACAACATAAACCGA+AGG - chr4_2:21183802-21183821 None:intergenic 35.0%
! CGGTTTTGCCAAAATAATCT+TGG + chr4_2:21183835-21183854 Msa0594120:CDS 35.0%
! GGTAAAGGGTTTGTCAATTT+TGG + chr4_2:21184038-21184057 Msa0594120:intron 35.0%
! TTGGATCCCCAATATTTTTC+TGG + chr4_2:21184131-21184150 Msa0594120:intron 35.0%
AATTAATGTCCGTAAACCGT+CGG - chr4_2:21184230-21184249 None:intergenic 35.0%
GGCATAAAGTGATACTAAAG+TGG + chr4_2:21184513-21184532 Msa0594120:intron 35.0%
!!! TAAAGTGGCTGCTTTTTATC+TGG + chr4_2:21184528-21184547 Msa0594120:intron 35.0%
TCATACTTTGCAAAAGTTGG+TGG + chr4_2:21184567-21184586 Msa0594120:CDS 35.0%
GCTGAAGAAATCAAAGAAAG+AGG + chr4_2:21184750-21184769 Msa0594120:CDS 35.0%
TTGAGTGTGAGTATCAATCT+TGG + chr4_2:21184876-21184895 Msa0594120:three_prime_UTR 35.0%
TGAGTGTGAGTATCAATCTT+GGG + chr4_2:21184877-21184896 Msa0594120:three_prime_UTR 35.0%
!!! AAGAAGGTTTTGAAAGGGAT+TGG - chr4_2:21183473-21183492 None:intergenic 35.0%
!! ATTTTTAGATACACACGTGC+TGG + chr4_2:21183775-21183794 Msa0594120:CDS 35.0%
TAAGCAACAACATAAACCGA+AGG - chr4_2:21183802-21183821 None:intergenic 35.0%
! CGGTTTTGCCAAAATAATCT+TGG + chr4_2:21183835-21183854 Msa0594120:CDS 35.0%
! GGTAAAGGGTTTGTCAATTT+TGG + chr4_2:21184038-21184057 Msa0594120:intron 35.0%
! TTGGATCCCCAATATTTTTC+TGG + chr4_2:21184131-21184150 Msa0594120:intron 35.0%
AATTAATGTCCGTAAACCGT+CGG - chr4_2:21184230-21184249 None:intergenic 35.0%
GGCATAAAGTGATACTAAAG+TGG + chr4_2:21184513-21184532 Msa0594120:intron 35.0%
!!! TAAAGTGGCTGCTTTTTATC+TGG + chr4_2:21184528-21184547 Msa0594120:intron 35.0%
TCATACTTTGCAAAAGTTGG+TGG + chr4_2:21184567-21184586 Msa0594120:CDS 35.0%
GCTGAAGAAATCAAAGAAAG+AGG + chr4_2:21184750-21184769 Msa0594120:CDS 35.0%
TTGAGTGTGAGTATCAATCT+TGG + chr4_2:21184876-21184895 Msa0594120:three_prime_UTR 35.0%
TGAGTGTGAGTATCAATCTT+GGG + chr4_2:21184877-21184896 Msa0594120:three_prime_UTR 35.0%
GGACTATTTGGATTGAGAGA+GGG - chr4_2:21183312-21183331 None:intergenic 40.0%
TGGACTATTTGGATTGAGAG+AGG - chr4_2:21183313-21183332 None:intergenic 40.0%
AGAATGGAGTTGTCAATGGA+GGG - chr4_2:21183351-21183370 None:intergenic 40.0%
AAGGAGAATGGAGTTGTCAA+TGG - chr4_2:21183355-21183374 None:intergenic 40.0%
CTCTTTAATCCGACGGTTTA+CGG + chr4_2:21184218-21184237 Msa0594120:intron 40.0%
TGAGAGCTATTGTTCCCATT+TGG + chr4_2:21184674-21184693 Msa0594120:CDS 40.0%
CTATTGTTCCCATTTGGAGA+GGG + chr4_2:21184680-21184699 Msa0594120:CDS 40.0%
GATTTCTTCAGCACACCTTA+AGG - chr4_2:21184742-21184761 None:intergenic 40.0%
GGACTATTTGGATTGAGAGA+GGG - chr4_2:21183312-21183331 None:intergenic 40.0%
TGGACTATTTGGATTGAGAG+AGG - chr4_2:21183313-21183332 None:intergenic 40.0%
AGAATGGAGTTGTCAATGGA+GGG - chr4_2:21183351-21183370 None:intergenic 40.0%
AAGGAGAATGGAGTTGTCAA+TGG - chr4_2:21183355-21183374 None:intergenic 40.0%
CTCTTTAATCCGACGGTTTA+CGG + chr4_2:21184218-21184237 Msa0594120:intron 40.0%
TGAGAGCTATTGTTCCCATT+TGG + chr4_2:21184674-21184693 Msa0594120:CDS 40.0%
CTATTGTTCCCATTTGGAGA+GGG + chr4_2:21184680-21184699 Msa0594120:CDS 40.0%
GATTTCTTCAGCACACCTTA+AGG - chr4_2:21184742-21184761 None:intergenic 40.0%
GAGAATGGAGTTGTCAATGG+AGG - chr4_2:21183352-21183371 None:intergenic 45.0%
TATTGGGGATCCAACATGCA+TGG - chr4_2:21184125-21184144 None:intergenic 45.0%
AATCTCCAATGGAGCCCTTT+CGG - chr4_2:21184447-21184466 None:intergenic 45.0%
GCTATTGTTCCCATTTGGAG+AGG + chr4_2:21184679-21184698 Msa0594120:CDS 45.0%
GCTTACTTCCCTCTCCAAAT+GGG - chr4_2:21184691-21184710 None:intergenic 45.0%
TGCTTACTTCCCTCTCCAAA+TGG - chr4_2:21184692-21184711 None:intergenic 45.0%
TTGGAGAGGGAAGTAAGCAT+TGG + chr4_2:21184693-21184712 Msa0594120:CDS 45.0%
ACCACATTGAGAGGACCTTA+AGG + chr4_2:21184724-21184743 Msa0594120:CDS 45.0%
ACCTTAAGGTCCTCTCAATG+TGG - chr4_2:21184728-21184747 None:intergenic 45.0%
ATTGGTACATCGCTTTCAGC+TGG - chr4_2:21185140-21185159 None:intergenic 45.0%
GAGAATGGAGTTGTCAATGG+AGG - chr4_2:21183352-21183371 None:intergenic 45.0%
TATTGGGGATCCAACATGCA+TGG - chr4_2:21184125-21184144 None:intergenic 45.0%
AATCTCCAATGGAGCCCTTT+CGG - chr4_2:21184447-21184466 None:intergenic 45.0%
GCTATTGTTCCCATTTGGAG+AGG + chr4_2:21184679-21184698 Msa0594120:CDS 45.0%
GCTTACTTCCCTCTCCAAAT+GGG - chr4_2:21184691-21184710 None:intergenic 45.0%
TGCTTACTTCCCTCTCCAAA+TGG - chr4_2:21184692-21184711 None:intergenic 45.0%
TTGGAGAGGGAAGTAAGCAT+TGG + chr4_2:21184693-21184712 Msa0594120:CDS 45.0%
ACCACATTGAGAGGACCTTA+AGG + chr4_2:21184724-21184743 Msa0594120:CDS 45.0%
ACCTTAAGGTCCTCTCAATG+TGG - chr4_2:21184728-21184747 None:intergenic 45.0%
ATTGGTACATCGCTTTCAGC+TGG - chr4_2:21185140-21185159 None:intergenic 45.0%
ATACACACGTGCTGGACCTT+CGG + chr4_2:21183783-21183802 Msa0594120:CDS 50.0%
TTGCCTCAAAGCATGGCCAA+TGG + chr4_2:21184099-21184118 Msa0594120:intron 50.0%
GATCCAACATGCATGGCCAT+TGG - chr4_2:21184118-21184137 None:intergenic 50.0%
TTTGACCGAAAGGGCTCCAT+TGG + chr4_2:21184439-21184458 Msa0594120:intron 50.0%
!!! GTGGCTGCTTTTTATCTGGC+AGG + chr4_2:21184532-21184551 Msa0594120:intron 50.0%
!! GAGAGGGAAGTAAGCATTGG+AGG + chr4_2:21184696-21184715 Msa0594120:CDS 50.0%
!! AGGGAAGTAAGCATTGGAGG+AGG + chr4_2:21184699-21184718 Msa0594120:CDS 50.0%
!! GGGAAGTAAGCATTGGAGGA+GGG + chr4_2:21184700-21184719 Msa0594120:CDS 50.0%
ATCTTGGGAGTGACAACTCG+AGG + chr4_2:21184892-21184911 Msa0594120:three_prime_UTR 50.0%
ATACACACGTGCTGGACCTT+CGG + chr4_2:21183783-21183802 Msa0594120:CDS 50.0%
TTGCCTCAAAGCATGGCCAA+TGG + chr4_2:21184099-21184118 Msa0594120:intron 50.0%
GATCCAACATGCATGGCCAT+TGG - chr4_2:21184118-21184137 None:intergenic 50.0%
TTTGACCGAAAGGGCTCCAT+TGG + chr4_2:21184439-21184458 Msa0594120:intron 50.0%
!!! GTGGCTGCTTTTTATCTGGC+AGG + chr4_2:21184532-21184551 Msa0594120:intron 50.0%
!! GAGAGGGAAGTAAGCATTGG+AGG + chr4_2:21184696-21184715 Msa0594120:CDS 50.0%
!! AGGGAAGTAAGCATTGGAGG+AGG + chr4_2:21184699-21184718 Msa0594120:CDS 50.0%
!! GGGAAGTAAGCATTGGAGGA+GGG + chr4_2:21184700-21184719 Msa0594120:CDS 50.0%
ATCTTGGGAGTGACAACTCG+AGG + chr4_2:21184892-21184911 Msa0594120:three_prime_UTR 50.0%
TGGCCATTGGCCATGCTTTG+AGG - chr4_2:21184105-21184124 None:intergenic 55.0%
TGGCCAATGGCCATGCATGT+TGG + chr4_2:21184112-21184131 Msa0594120:intron 55.0%
!! GAGGAGGGTACCACATTGAG+AGG + chr4_2:21184715-21184734 Msa0594120:CDS 55.0%
TGGCCATTGGCCATGCTTTG+AGG - chr4_2:21184105-21184124 None:intergenic 55.0%
TGGCCAATGGCCATGCATGT+TGG + chr4_2:21184112-21184131 Msa0594120:intron 55.0%
!! GAGGAGGGTACCACATTGAG+AGG + chr4_2:21184715-21184734 Msa0594120:CDS 55.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 21183306 21185219 21183306 ID=Msa0594120;Name=Msa0594120
chr4_2 mRNA 21183306 21185219 21183306 ID=Msa0594120-mRNA-1;Parent=Msa0594120;Name=Msa0594120-mRNA-1;_AED=0.01;_eAED=0.01;_QI=223|1|1|1|1|1|2|416|218
chr4_2 exon 21183306 21183935 21183306 ID=Msa0594120-mRNA-1:exon:5904;Parent=Msa0594120-mRNA-1
chr4_2 exon 21184554 21185219 21184554 ID=Msa0594120-mRNA-1:exon:5905;Parent=Msa0594120-mRNA-1
chr4_2 five_prime_UTR 21183306 21183528 21183306 ID=Msa0594120-mRNA-1:five_prime_utr;Parent=Msa0594120-mRNA-1
chr4_2 CDS 21183529 21183935 21183529 ID=Msa0594120-mRNA-1:cds;Parent=Msa0594120-mRNA-1
chr4_2 CDS 21184554 21184803 21184554 ID=Msa0594120-mRNA-1:cds;Parent=Msa0594120-mRNA-1
chr4_2 three_prime_UTR 21184804 21185219 21184804 ID=Msa0594120-mRNA-1:three_prime_utr;Parent=Msa0594120-mRNA-1
Gene Sequence

>Msa0594120

ATGGATTCTTCTTCTTTGACAATATCTCCAAGAAAACTACACTATGATTTATATTCCTATTCATACAAAGAAGATTCAAACACCCCATTAGTGATAAATGTTCTAGCTTCATTAATTGAGAGAAACATGGCAAGAGCACAAAGAATTGTGAAGAATTGTTCTTCAAGAGTTTTGTCCAAAGCAAGCACAAAAATCTTTGATTGTAGAGAGATTCCTGATTTAACCATTCAATCTTATTTAGAGAGAATTTTTAGATACACACGTGCTGGACCTTCGGTTTATGTTGTTGCTTATGTCTATATTGATCGGTTTTGCCAAAATAATCTTGGGTTTAGAATTAATTCAAGAAATGTTCATAGACTTCTCATAACAACTATTATGGTTGCTTCAAAGTACGTTGAAGATATGAATTTCAGAAATTCATACTTTGCAAAAGTTGGTGGTTTAACAACAAATGAATTGAATGAGTTGGAGCTTGAATTTCTTTTCATGATGAATTTCAAATTACATGTTAATGTGAGTGTATTTGAGAGCTATTGTTCCCATTTGGAGAGGGAAGTAAGCATTGGAGGAGGGTACCACATTGAGAGGACCTTAAGGTGTGCTGAAGAAATCAAAGAAAGAGGTCACACACAAATTGCACGTGTAATGTTGTAG
Protein sequence

>Msa0594120

MDSSSLTISPRKLHYDLYSYSYKEDSNTPLVINVLASLIERNMARAQRIVKNCSSRVLSKASTKIFDCREIPDLTIQSYLERIFRYTRAGPSVYVVAYVYIDRFCQNNLGFRINSRNVHRLLITTIMVASKYVEDMNFRNSYFAKVGGLTTNELNELELEFLFMMNFKLHVNVSVFESYCSHLEREVSIGGGYHIERTLRCAEEIKERGHTQIARVML*