AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0537760

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0537760 MtrunA17_Chr4g0012811 99.541 218 1 0 1 218 1 218 2.01e-162 447
Msa0537760 MtrunA17_Chr7g0231431 83.111 225 30 3 1 218 1 224 4.83e-134 375
Msa0537760 MtrunA17_Chr7g0250121 46.541 159 79 2 29 187 11 163 8.07e-46 150
Msa0537760 MtrunA17_Chr8g0335971 45.732 164 81 1 24 187 5 160 1.02e-45 150
Msa0537760 MtrunA17_Chr7g0259831 47.541 122 63 1 65 186 63 183 3.00e-37 129
Msa0537760 MtrunA17_Chr2g0322921 43.169 183 93 3 27 199 24 205 1.40e-33 120
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0537760 AT2G45080.1 72.768 224 47 3 5 218 3 222 1.08e-114 327
Msa0537760 AT3G60550.1 70.044 227 57 1 3 218 4 230 4.25e-113 323
Msa0537760 AT2G44740.1 44.654 159 84 2 31 189 13 167 1.08e-43 145
Msa0537760 AT5G07450.1 40.351 171 86 2 29 193 21 181 6.87e-41 139
Msa0537760 AT5G61650.1 40.373 161 88 1 29 189 23 175 6.25e-39 134
Msa0537760 AT3G63120.2 42.958 142 78 2 59 200 58 196 2.79e-38 132
Msa0537760 AT3G63120.1 42.958 142 78 2 59 200 58 196 2.79e-38 132
Msa0537760 AT3G21870.1 39.394 165 97 2 25 189 21 182 1.15e-35 125
Msa0537760 AT3G05327.1 37.725 167 97 2 25 187 27 190 5.91e-33 118

Find 50 sgRNAs with CRISPR-Local

Find 226 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTAAACCCAAGATTATTT+TGG 0.173257 4_1:+16665235 None:intergenic
AGATAATTATTTGGACTATT+AGG 0.193517 4_1:+16665757 None:intergenic
TTTGTTGTTAAGAAATTATT+TGG 0.237058 4_1:+16665664 None:intergenic
GAGGGAGAAAGATAATTATT+TGG 0.256810 4_1:+16665748 None:intergenic
ACTTCTCATAACAACTATTA+TGG 0.285093 4_1:-16665190 Msa0537760:CDS
TGTGATGGAACAATACAAAT+TGG 0.286229 4_1:+16663925 None:intergenic
GCTAGAACATTTATCACTAA+TGG 0.307122 4_1:+16665465 None:intergenic
GGAGTGGAATTTATATATAA+AGG 0.322270 4_1:+16665707 None:intergenic
TGAATCTTCTTTGTATGAAT+AGG 0.344032 4_1:+16665494 None:intergenic
CTAGAACATTTATCACTAAT+GGG 0.347684 4_1:+16665466 None:intergenic
TGAGAGCTATTGTTCCCATT+TGG 0.387926 4_1:-16664405 Msa0537760:CDS
AATTCATACTTTGCAAAAGT+TGG 0.389350 4_1:-16664515 Msa0537760:CDS
GATTTCTTCAGCACACCTTA+AGG 0.402870 4_1:+16664340 None:intergenic
TACTCACACTCAATAATATA+CGG 0.406412 4_1:+16664213 None:intergenic
TGCTTACTTCCCTCTCCAAA+TGG 0.411186 4_1:+16664390 None:intergenic
TAAGATTGAATGGTTAAATC+AGG 0.411388 4_1:+16665336 None:intergenic
TCTCTCTAAATAAGATTGAA+TGG 0.421099 4_1:+16665326 None:intergenic
GAGAGGGAAGTAAGCATTGG+AGG 0.422508 4_1:-16664383 Msa0537760:CDS
AATTCATTGTTTCTTTGTGA+TGG 0.426845 4_1:+16663910 None:intergenic
ATACACACGTGCTGGACCTT+CGG 0.437594 4_1:-16665295 Msa0537760:CDS
TATATAAAGGAGAATGGAGT+TGG 0.439002 4_1:+16665720 None:intergenic
TAAATTTAAATGTTTGTTGA+GGG 0.442331 4_1:-16663994 Msa0537760:three_prime_UTR
AGGGAAGTAAGCATTGGAGG+AGG 0.444484 4_1:-16664380 Msa0537760:CDS
GAGAATGGAGTTGGCAATGG+AGG 0.446864 4_1:+16665729 None:intergenic
TTGAGTGTGAGTATCAATCT+TGG 0.464497 4_1:-16664203 Msa0537760:three_prime_UTR
TTGCTTATGTCTATATTGAT+CGG 0.466190 4_1:-16665263 Msa0537760:CDS
TGAGTGTGAGTATCAATCTT+GGG 0.485348 4_1:-16664202 Msa0537760:three_prime_UTR
TCATACTTTGCAAAAGTTGG+TGG 0.491361 4_1:-16664512 Msa0537760:CDS
AACAAATGAATTGAATGAGT+TGG 0.500361 4_1:-16664483 Msa0537760:CDS
ATTGGTACATCGCTTTCAGC+TGG 0.502137 4_1:+16663943 None:intergenic
ACCACATTGAGAGGACCTTA+AGG 0.502225 4_1:-16664355 Msa0537760:CDS
AAGGAGAATGGAGTTGGCAA+TGG 0.503207 4_1:+16665726 None:intergenic
GTAAATTTAAATGTTTGTTG+AGG 0.514689 4_1:-16663995 Msa0537760:three_prime_UTR
AGAATGGAGTTGGCAATGGA+GGG 0.519610 4_1:+16665730 None:intergenic
GCTGAAGAAATCAAAGAAAG+AGG 0.532244 4_1:-16664329 Msa0537760:CDS
TTGGAGAGGGAAGTAAGCAT+TGG 0.532407 4_1:-16664386 Msa0537760:CDS
GCTTACTTCCCTCTCCAAAT+GGG 0.534304 4_1:+16664391 None:intergenic
AATTTATATATAAAGGAGAA+TGG 0.542353 4_1:+16665714 None:intergenic
TGGACTATTAGGATTGAGAG+AGG 0.563175 4_1:+16665768 None:intergenic
AAATTTAAATGTTTGTTGAG+GGG 0.590012 4_1:-16663993 Msa0537760:three_prime_UTR
GGGAAGTAAGCATTGGAGGA+GGG 0.593739 4_1:-16664379 Msa0537760:CDS
CTATTGTTCCCATTTGGAGA+GGG 0.594811 4_1:-16664399 Msa0537760:CDS
GGACTATTAGGATTGAGAGA+GGG 0.614020 4_1:+16665769 None:intergenic
GCTATTGTTCCCATTTGGAG+AGG 0.621485 4_1:-16664400 Msa0537760:CDS
GAGGAGGGTACCACATTGAG+AGG 0.624143 4_1:-16664364 Msa0537760:CDS
TTCATTAATTGAGAGAAACA+TGG 0.648460 4_1:-16665442 Msa0537760:CDS
TAGAACATTTATCACTAATG+GGG 0.651890 4_1:+16665467 None:intergenic
ACCTTAAGGTCCTCTCAATG+TGG 0.678220 4_1:+16664354 None:intergenic
TAAGCAACAACATAAACCGA+AGG 0.681604 4_1:+16665279 None:intergenic
ATCTTGGGAGAGACAACTCG+AGG 0.694846 4_1:-16664187 Msa0537760:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTAATTTAACAACAAATA+TGG - chr4_1:16664973-16664992 Msa0537760:intron 10.0%
!!! AAAAAATTTCTCTTTATTTT+AGG - chr4_1:16665585-16665604 Msa0537760:five_prime_UTR 10.0%
!! AATTAATTTAACAACAAATA+TGG - chr4_1:16664973-16664992 Msa0537760:intron 10.0%
!!! AAAAAATTTCTCTTTATTTT+AGG - chr4_1:16665585-16665604 Msa0537760:five_prime_UTR 10.0%
!! AATTTATATATAAAGGAGAA+TGG + chr4_1:16663944-16663963 None:intergenic 15.0%
!!! TTTGTTGTTAAGAAATTATT+TGG + chr4_1:16663994-16664013 None:intergenic 15.0%
!!! AATTTCTTACATTATTTTCA+AGG - chr4_1:16664583-16664602 Msa0537760:intron 15.0%
!! TTGTTTATTAAAATCTCAAT+CGG - chr4_1:16664645-16664664 Msa0537760:intron 15.0%
!! ATTAATGGTCAAATTTAATT+TGG - chr4_1:16664828-16664847 Msa0537760:intron 15.0%
!!! ATAAACATCTTGAATTTTAA+TGG + chr4_1:16664921-16664940 None:intergenic 15.0%
!!! TTTCTATATTTAGTTTATTG+TGG - chr4_1:16665068-16665087 Msa0537760:intron 15.0%
!! AAAAAAAAAAAAAAACTCGA+GGG - chr4_1:16665504-16665523 Msa0537760:CDS 15.0%
!!! TTTTTTTTCTTGCAAAATAA+AGG - chr4_1:16665611-16665630 Msa0537760:five_prime_UTR 15.0%
!!! TAAATTTAAATGTTTGTTGA+GGG - chr4_1:16665661-16665680 Msa0537760:five_prime_UTR 15.0%
!! AATTTATATATAAAGGAGAA+TGG + chr4_1:16663944-16663963 None:intergenic 15.0%
!!! TTTGTTGTTAAGAAATTATT+TGG + chr4_1:16663994-16664013 None:intergenic 15.0%
!!! AATTTCTTACATTATTTTCA+AGG - chr4_1:16664583-16664602 Msa0537760:intron 15.0%
!! TTGTTTATTAAAATCTCAAT+CGG - chr4_1:16664645-16664664 Msa0537760:intron 15.0%
!! ATTAATGGTCAAATTTAATT+TGG - chr4_1:16664828-16664847 Msa0537760:intron 15.0%
!!! ATAAACATCTTGAATTTTAA+TGG + chr4_1:16664921-16664940 None:intergenic 15.0%
!!! TTTCTATATTTAGTTTATTG+TGG - chr4_1:16665068-16665087 Msa0537760:intron 15.0%
!! AAAAAAAAAAAAAAACTCGA+GGG - chr4_1:16665504-16665523 Msa0537760:CDS 15.0%
!!! TTTTTTTTCTTGCAAAATAA+AGG - chr4_1:16665611-16665630 Msa0537760:five_prime_UTR 15.0%
!!! TAAATTTAAATGTTTGTTGA+GGG - chr4_1:16665661-16665680 Msa0537760:five_prime_UTR 15.0%
!! AGATAATTATTTGGACTATT+AGG + chr4_1:16663901-16663920 None:intergenic 20.0%
!! AAAACAAATAGAGAAAAAGA+AGG + chr4_1:16664533-16664552 None:intergenic 20.0%
!!! TTATTTTCAAGGTAACAATT+TGG - chr4_1:16664594-16664613 Msa0537760:intron 20.0%
!!! AATGTATTTCTTTTAATCTG+AGG + chr4_1:16664767-16664786 None:intergenic 20.0%
!!! TTTTTTTCTCTTTAATCTGA+CGG - chr4_1:16664787-16664806 Msa0537760:intron 20.0%
!!! TTTTTTTTTTTGAGAGATCT+CGG + chr4_1:16665495-16665514 None:intergenic 20.0%
!! CAAAAAAAAAAAAAAACTCG+AGG - chr4_1:16665503-16665522 Msa0537760:CDS 20.0%
!!! AGTTTTAAATAGTTTTCTCT+CGG + chr4_1:16665530-16665549 None:intergenic 20.0%
!! ACTGAAAAATCATAGATATA+TGG - chr4_1:16665547-16665566 Msa0537760:CDS 20.0%
!!! GTAAATTTAAATGTTTGTTG+AGG - chr4_1:16665660-16665679 Msa0537760:five_prime_UTR 20.0%
!!! AAATTTAAATGTTTGTTGAG+GGG - chr4_1:16665662-16665681 Msa0537760:five_prime_UTR 20.0%
!! AGATAATTATTTGGACTATT+AGG + chr4_1:16663901-16663920 None:intergenic 20.0%
!! AAAACAAATAGAGAAAAAGA+AGG + chr4_1:16664533-16664552 None:intergenic 20.0%
!!! TTATTTTCAAGGTAACAATT+TGG - chr4_1:16664594-16664613 Msa0537760:intron 20.0%
!!! AATGTATTTCTTTTAATCTG+AGG + chr4_1:16664767-16664786 None:intergenic 20.0%
!!! TTTTTTTCTCTTTAATCTGA+CGG - chr4_1:16664787-16664806 Msa0537760:intron 20.0%
!!! TTTTTTTTTTTGAGAGATCT+CGG + chr4_1:16665495-16665514 None:intergenic 20.0%
!! CAAAAAAAAAAAAAAACTCG+AGG - chr4_1:16665503-16665522 Msa0537760:CDS 20.0%
!!! AGTTTTAAATAGTTTTCTCT+CGG + chr4_1:16665530-16665549 None:intergenic 20.0%
!! ACTGAAAAATCATAGATATA+TGG - chr4_1:16665547-16665566 Msa0537760:CDS 20.0%
!!! GTAAATTTAAATGTTTGTTG+AGG - chr4_1:16665660-16665679 Msa0537760:five_prime_UTR 20.0%
!!! AAATTTAAATGTTTGTTGAG+GGG - chr4_1:16665662-16665681 Msa0537760:five_prime_UTR 20.0%
! GGAGTGGAATTTATATATAA+AGG + chr4_1:16663951-16663970 None:intergenic 25.0%
!!! TCTTTTTTTCTTGTAGAAGA+AGG + chr4_1:16664066-16664085 None:intergenic 25.0%
! AAGAAGAATCCATCAAAAAA+GGG + chr4_1:16664099-16664118 None:intergenic 25.0%
!! AAATCATAGTGTAGTTTTCT+TGG + chr4_1:16664136-16664155 None:intergenic 25.0%
! TGAATCTTCTTTGTATGAAT+AGG + chr4_1:16664164-16664183 None:intergenic 25.0%
! TAGAACATTTATCACTAATG+GGG + chr4_1:16664191-16664210 None:intergenic 25.0%
! CTAGAACATTTATCACTAAT+GGG + chr4_1:16664192-16664211 None:intergenic 25.0%
! TTCATTAATTGAGAGAAACA+TGG - chr4_1:16664213-16664232 Msa0537760:three_prime_UTR 25.0%
! TAAGATTGAATGGTTAAATC+AGG + chr4_1:16664322-16664341 None:intergenic 25.0%
! TCTCTCTAAATAAGATTGAA+TGG + chr4_1:16664332-16664351 None:intergenic 25.0%
! TTGCTTATGTCTATATTGAT+CGG - chr4_1:16664392-16664411 Msa0537760:CDS 25.0%
!! TTCTAAACCCAAGATTATTT+TGG + chr4_1:16664423-16664442 None:intergenic 25.0%
! ACTTCTCATAACAACTATTA+TGG - chr4_1:16664465-16664484 Msa0537760:CDS 25.0%
!! TTCTTTTAATCTGAGGATTA+CGG + chr4_1:16664760-16664779 None:intergenic 25.0%
!!! ATTTTAATGGTAGAGAATCT+AGG + chr4_1:16664908-16664927 None:intergenic 25.0%
!!! TTTTTTGTCAAAATCTCCAA+TGG + chr4_1:16665034-16665053 None:intergenic 25.0%
! AATTCATACTTTGCAAAAGT+TGG - chr4_1:16665140-16665159 Msa0537760:intron 25.0%
! AACAAATGAATTGAATGAGT+TGG - chr4_1:16665172-16665191 Msa0537760:CDS 25.0%
!! TGTAATGTTGTAGACTTTTT+AGG - chr4_1:16665367-16665386 Msa0537760:CDS 25.0%
!! GTAATGTTGTAGACTTTTTA+GGG - chr4_1:16665368-16665387 Msa0537760:CDS 25.0%
! TACTCACACTCAATAATATA+CGG + chr4_1:16665445-16665464 None:intergenic 25.0%
!! TTTTCTTGCAAAATAAAGGA+AGG - chr4_1:16665615-16665634 Msa0537760:five_prime_UTR 25.0%
!!! GATTGAGATTTTAGAACTAT+AGG - chr4_1:16665684-16665703 Msa0537760:five_prime_UTR 25.0%
!! AATTCATTGTTTCTTTGTGA+TGG + chr4_1:16665748-16665767 None:intergenic 25.0%
! GGAGTGGAATTTATATATAA+AGG + chr4_1:16663951-16663970 None:intergenic 25.0%
!!! TCTTTTTTTCTTGTAGAAGA+AGG + chr4_1:16664066-16664085 None:intergenic 25.0%
! AAGAAGAATCCATCAAAAAA+GGG + chr4_1:16664099-16664118 None:intergenic 25.0%
!! AAATCATAGTGTAGTTTTCT+TGG + chr4_1:16664136-16664155 None:intergenic 25.0%
! TGAATCTTCTTTGTATGAAT+AGG + chr4_1:16664164-16664183 None:intergenic 25.0%
! TAGAACATTTATCACTAATG+GGG + chr4_1:16664191-16664210 None:intergenic 25.0%
! CTAGAACATTTATCACTAAT+GGG + chr4_1:16664192-16664211 None:intergenic 25.0%
! TTCATTAATTGAGAGAAACA+TGG - chr4_1:16664213-16664232 Msa0537760:three_prime_UTR 25.0%
! TAAGATTGAATGGTTAAATC+AGG + chr4_1:16664322-16664341 None:intergenic 25.0%
! TCTCTCTAAATAAGATTGAA+TGG + chr4_1:16664332-16664351 None:intergenic 25.0%
! TTGCTTATGTCTATATTGAT+CGG - chr4_1:16664392-16664411 Msa0537760:CDS 25.0%
!! TTCTAAACCCAAGATTATTT+TGG + chr4_1:16664423-16664442 None:intergenic 25.0%
! ACTTCTCATAACAACTATTA+TGG - chr4_1:16664465-16664484 Msa0537760:CDS 25.0%
!! TTCTTTTAATCTGAGGATTA+CGG + chr4_1:16664760-16664779 None:intergenic 25.0%
!!! ATTTTAATGGTAGAGAATCT+AGG + chr4_1:16664908-16664927 None:intergenic 25.0%
!!! TTTTTTGTCAAAATCTCCAA+TGG + chr4_1:16665034-16665053 None:intergenic 25.0%
! AATTCATACTTTGCAAAAGT+TGG - chr4_1:16665140-16665159 Msa0537760:intron 25.0%
! AACAAATGAATTGAATGAGT+TGG - chr4_1:16665172-16665191 Msa0537760:CDS 25.0%
!! TGTAATGTTGTAGACTTTTT+AGG - chr4_1:16665367-16665386 Msa0537760:CDS 25.0%
!! GTAATGTTGTAGACTTTTTA+GGG - chr4_1:16665368-16665387 Msa0537760:CDS 25.0%
! TACTCACACTCAATAATATA+CGG + chr4_1:16665445-16665464 None:intergenic 25.0%
!! TTTTCTTGCAAAATAAAGGA+AGG - chr4_1:16665615-16665634 Msa0537760:five_prime_UTR 25.0%
!!! GATTGAGATTTTAGAACTAT+AGG - chr4_1:16665684-16665703 Msa0537760:five_prime_UTR 25.0%
!! AATTCATTGTTTCTTTGTGA+TGG + chr4_1:16665748-16665767 None:intergenic 25.0%
GAGGGAGAAAGATAATTATT+TGG + chr4_1:16663910-16663929 None:intergenic 30.0%
TATATAAAGGAGAATGGAGT+TGG + chr4_1:16663938-16663957 None:intergenic 30.0%
!!! AATGAAGAAAGTTTTAGGAG+TGG + chr4_1:16663967-16663986 None:intergenic 30.0%
! GAAGCAATGAAGAAAGTTTT+AGG + chr4_1:16663972-16663991 None:intergenic 30.0%
!!! TGTAGAAGAAGGTTTTGAAA+GGG + chr4_1:16664055-16664074 None:intergenic 30.0%
!!! TTGTAGAAGAAGGTTTTGAA+AGG + chr4_1:16664056-16664075 None:intergenic 30.0%
!!! GAAAATTTCCCCTTTTTTGA+TGG - chr4_1:16664087-16664106 Msa0537760:three_prime_UTR 30.0%
AGAAGAATCCATCAAAAAAG+GGG + chr4_1:16664098-16664117 None:intergenic 30.0%
GAAGAAGAATCCATCAAAAA+AGG + chr4_1:16664100-16664119 None:intergenic 30.0%
GCTAGAACATTTATCACTAA+TGG + chr4_1:16664193-16664212 None:intergenic 30.0%
! AAAGATTTTTGTGCTTGCTT+TGG + chr4_1:16664284-16664303 None:intergenic 30.0%
! GGTTTTGCCAAAATAATCTT+GGG - chr4_1:16664413-16664432 Msa0537760:CDS 30.0%
TCAAGGTAACAATTTGGTAA+AGG - chr4_1:16664600-16664619 Msa0537760:intron 30.0%
CAAGGTAACAATTTGGTAAA+GGG - chr4_1:16664601-16664620 Msa0537760:intron 30.0%
AATTATATTGCCTCAAAGCA+TGG - chr4_1:16664669-16664688 Msa0537760:intron 30.0%
TGAAGACCAGAAAAATATTG+GGG + chr4_1:16664717-16664736 None:intergenic 30.0%
GTGAAGACCAGAAAAATATT+GGG + chr4_1:16664718-16664737 None:intergenic 30.0%
AGTGAAGACCAGAAAAATAT+TGG + chr4_1:16664719-16664738 None:intergenic 30.0%
ACGGACATTAATTGCATTAA+TGG - chr4_1:16664813-16664832 Msa0537760:intron 30.0%
!! TAACTATCATTTTGACCGAA+AGG - chr4_1:16665005-16665024 Msa0537760:intron 30.0%
!! AACTATCATTTTGACCGAAA+GGG - chr4_1:16665006-16665025 Msa0537760:intron 30.0%
!!! AGATTTTAGAACTATAGGTG+AGG - chr4_1:16665689-16665708 Msa0537760:five_prime_UTR 30.0%
! TGTGATGGAACAATACAAAT+TGG + chr4_1:16665733-16665752 None:intergenic 30.0%
! CAATGAATTTTATGAGCAAG+AGG - chr4_1:16665759-16665778 Msa0537760:five_prime_UTR 30.0%
GAGGGAGAAAGATAATTATT+TGG + chr4_1:16663910-16663929 None:intergenic 30.0%
TATATAAAGGAGAATGGAGT+TGG + chr4_1:16663938-16663957 None:intergenic 30.0%
!!! AATGAAGAAAGTTTTAGGAG+TGG + chr4_1:16663967-16663986 None:intergenic 30.0%
! GAAGCAATGAAGAAAGTTTT+AGG + chr4_1:16663972-16663991 None:intergenic 30.0%
!!! TGTAGAAGAAGGTTTTGAAA+GGG + chr4_1:16664055-16664074 None:intergenic 30.0%
!!! TTGTAGAAGAAGGTTTTGAA+AGG + chr4_1:16664056-16664075 None:intergenic 30.0%
!!! GAAAATTTCCCCTTTTTTGA+TGG - chr4_1:16664087-16664106 Msa0537760:three_prime_UTR 30.0%
AGAAGAATCCATCAAAAAAG+GGG + chr4_1:16664098-16664117 None:intergenic 30.0%
GAAGAAGAATCCATCAAAAA+AGG + chr4_1:16664100-16664119 None:intergenic 30.0%
GCTAGAACATTTATCACTAA+TGG + chr4_1:16664193-16664212 None:intergenic 30.0%
! AAAGATTTTTGTGCTTGCTT+TGG + chr4_1:16664284-16664303 None:intergenic 30.0%
! GGTTTTGCCAAAATAATCTT+GGG - chr4_1:16664413-16664432 Msa0537760:CDS 30.0%
TCAAGGTAACAATTTGGTAA+AGG - chr4_1:16664600-16664619 Msa0537760:intron 30.0%
CAAGGTAACAATTTGGTAAA+GGG - chr4_1:16664601-16664620 Msa0537760:intron 30.0%
AATTATATTGCCTCAAAGCA+TGG - chr4_1:16664669-16664688 Msa0537760:intron 30.0%
TGAAGACCAGAAAAATATTG+GGG + chr4_1:16664717-16664736 None:intergenic 30.0%
GTGAAGACCAGAAAAATATT+GGG + chr4_1:16664718-16664737 None:intergenic 30.0%
AGTGAAGACCAGAAAAATAT+TGG + chr4_1:16664719-16664738 None:intergenic 30.0%
ACGGACATTAATTGCATTAA+TGG - chr4_1:16664813-16664832 Msa0537760:intron 30.0%
!! TAACTATCATTTTGACCGAA+AGG - chr4_1:16665005-16665024 Msa0537760:intron 30.0%
!! AACTATCATTTTGACCGAAA+GGG - chr4_1:16665006-16665025 Msa0537760:intron 30.0%
!!! AGATTTTAGAACTATAGGTG+AGG - chr4_1:16665689-16665708 Msa0537760:five_prime_UTR 30.0%
! TGTGATGGAACAATACAAAT+TGG + chr4_1:16665733-16665752 None:intergenic 30.0%
! CAATGAATTTTATGAGCAAG+AGG - chr4_1:16665759-16665778 Msa0537760:five_prime_UTR 30.0%
!!! AAGAAGGTTTTGAAAGGGAT+TGG + chr4_1:16664050-16664069 None:intergenic 35.0%
!! ATTTTTAGATACACACGTGC+TGG - chr4_1:16664352-16664371 Msa0537760:CDS 35.0%
TAAGCAACAACATAAACCGA+AGG + chr4_1:16664379-16664398 None:intergenic 35.0%
! CGGTTTTGCCAAAATAATCT+TGG - chr4_1:16664412-16664431 Msa0537760:CDS 35.0%
! GGTAAAGGGTTTGTCAATTT+TGG - chr4_1:16664615-16664634 Msa0537760:intron 35.0%
! TTGGATCCCCAATATTTTTC+TGG - chr4_1:16664708-16664727 Msa0537760:intron 35.0%
CTCTTTAATCTGACGGTTTA+CGG - chr4_1:16664794-16664813 Msa0537760:intron 35.0%
GGCATAAAGTGATACTAAAG+TGG - chr4_1:16665089-16665108 Msa0537760:intron 35.0%
!!! TAAAGTGGCTGCTTTTTATC+TGG - chr4_1:16665104-16665123 Msa0537760:intron 35.0%
TCATACTTTGCAAAAGTTGG+TGG - chr4_1:16665143-16665162 Msa0537760:intron 35.0%
GCTGAAGAAATCAAAGAAAG+AGG - chr4_1:16665326-16665345 Msa0537760:CDS 35.0%
TTGAGTGTGAGTATCAATCT+TGG - chr4_1:16665452-16665471 Msa0537760:CDS 35.0%
TGAGTGTGAGTATCAATCTT+GGG - chr4_1:16665453-16665472 Msa0537760:CDS 35.0%
!!! AAGAAGGTTTTGAAAGGGAT+TGG + chr4_1:16664050-16664069 None:intergenic 35.0%
!! ATTTTTAGATACACACGTGC+TGG - chr4_1:16664352-16664371 Msa0537760:CDS 35.0%
TAAGCAACAACATAAACCGA+AGG + chr4_1:16664379-16664398 None:intergenic 35.0%
! CGGTTTTGCCAAAATAATCT+TGG - chr4_1:16664412-16664431 Msa0537760:CDS 35.0%
! GGTAAAGGGTTTGTCAATTT+TGG - chr4_1:16664615-16664634 Msa0537760:intron 35.0%
! TTGGATCCCCAATATTTTTC+TGG - chr4_1:16664708-16664727 Msa0537760:intron 35.0%
CTCTTTAATCTGACGGTTTA+CGG - chr4_1:16664794-16664813 Msa0537760:intron 35.0%
GGCATAAAGTGATACTAAAG+TGG - chr4_1:16665089-16665108 Msa0537760:intron 35.0%
!!! TAAAGTGGCTGCTTTTTATC+TGG - chr4_1:16665104-16665123 Msa0537760:intron 35.0%
TCATACTTTGCAAAAGTTGG+TGG - chr4_1:16665143-16665162 Msa0537760:intron 35.0%
GCTGAAGAAATCAAAGAAAG+AGG - chr4_1:16665326-16665345 Msa0537760:CDS 35.0%
TTGAGTGTGAGTATCAATCT+TGG - chr4_1:16665452-16665471 Msa0537760:CDS 35.0%
TGAGTGTGAGTATCAATCTT+GGG - chr4_1:16665453-16665472 Msa0537760:CDS 35.0%
GGACTATTAGGATTGAGAGA+GGG + chr4_1:16663889-16663908 None:intergenic 40.0%
TGGACTATTAGGATTGAGAG+AGG + chr4_1:16663890-16663909 None:intergenic 40.0%
TGAGAGCTATTGTTCCCATT+TGG - chr4_1:16665250-16665269 Msa0537760:CDS 40.0%
CTATTGTTCCCATTTGGAGA+GGG - chr4_1:16665256-16665275 Msa0537760:CDS 40.0%
GATTTCTTCAGCACACCTTA+AGG + chr4_1:16665318-16665337 None:intergenic 40.0%
GGACTATTAGGATTGAGAGA+GGG + chr4_1:16663889-16663908 None:intergenic 40.0%
TGGACTATTAGGATTGAGAG+AGG + chr4_1:16663890-16663909 None:intergenic 40.0%
TGAGAGCTATTGTTCCCATT+TGG - chr4_1:16665250-16665269 Msa0537760:CDS 40.0%
CTATTGTTCCCATTTGGAGA+GGG - chr4_1:16665256-16665275 Msa0537760:CDS 40.0%
GATTTCTTCAGCACACCTTA+AGG + chr4_1:16665318-16665337 None:intergenic 40.0%
AGAATGGAGTTGGCAATGGA+GGG + chr4_1:16663928-16663947 None:intergenic 45.0%
AAGGAGAATGGAGTTGGCAA+TGG + chr4_1:16663932-16663951 None:intergenic 45.0%
TATTGGGGATCCAACATGCA+TGG + chr4_1:16664702-16664721 None:intergenic 45.0%
AATCTCCAATGGAGCCCTTT+CGG + chr4_1:16665023-16665042 None:intergenic 45.0%
GCTATTGTTCCCATTTGGAG+AGG - chr4_1:16665255-16665274 Msa0537760:CDS 45.0%
GCTTACTTCCCTCTCCAAAT+GGG + chr4_1:16665267-16665286 None:intergenic 45.0%
TGCTTACTTCCCTCTCCAAA+TGG + chr4_1:16665268-16665287 None:intergenic 45.0%
TTGGAGAGGGAAGTAAGCAT+TGG - chr4_1:16665269-16665288 Msa0537760:CDS 45.0%
ACCACATTGAGAGGACCTTA+AGG - chr4_1:16665300-16665319 Msa0537760:CDS 45.0%
ACCTTAAGGTCCTCTCAATG+TGG + chr4_1:16665304-16665323 None:intergenic 45.0%
ATTGGTACATCGCTTTCAGC+TGG + chr4_1:16665715-16665734 None:intergenic 45.0%
AGAATGGAGTTGGCAATGGA+GGG + chr4_1:16663928-16663947 None:intergenic 45.0%
AAGGAGAATGGAGTTGGCAA+TGG + chr4_1:16663932-16663951 None:intergenic 45.0%
TATTGGGGATCCAACATGCA+TGG + chr4_1:16664702-16664721 None:intergenic 45.0%
AATCTCCAATGGAGCCCTTT+CGG + chr4_1:16665023-16665042 None:intergenic 45.0%
GCTATTGTTCCCATTTGGAG+AGG - chr4_1:16665255-16665274 Msa0537760:CDS 45.0%
GCTTACTTCCCTCTCCAAAT+GGG + chr4_1:16665267-16665286 None:intergenic 45.0%
TGCTTACTTCCCTCTCCAAA+TGG + chr4_1:16665268-16665287 None:intergenic 45.0%
TTGGAGAGGGAAGTAAGCAT+TGG - chr4_1:16665269-16665288 Msa0537760:CDS 45.0%
ACCACATTGAGAGGACCTTA+AGG - chr4_1:16665300-16665319 Msa0537760:CDS 45.0%
ACCTTAAGGTCCTCTCAATG+TGG + chr4_1:16665304-16665323 None:intergenic 45.0%
ATTGGTACATCGCTTTCAGC+TGG + chr4_1:16665715-16665734 None:intergenic 45.0%
GAGAATGGAGTTGGCAATGG+AGG + chr4_1:16663929-16663948 None:intergenic 50.0%
ATACACACGTGCTGGACCTT+CGG - chr4_1:16664360-16664379 Msa0537760:CDS 50.0%
TTGCCTCAAAGCATGGCCAA+TGG - chr4_1:16664676-16664695 Msa0537760:intron 50.0%
GATCCAACATGCATGGCCAT+TGG + chr4_1:16664695-16664714 None:intergenic 50.0%
TTTGACCGAAAGGGCTCCAT+TGG - chr4_1:16665015-16665034 Msa0537760:intron 50.0%
!!! GTGGCTGCTTTTTATCTGGC+AGG - chr4_1:16665108-16665127 Msa0537760:intron 50.0%
!! GAGAGGGAAGTAAGCATTGG+AGG - chr4_1:16665272-16665291 Msa0537760:CDS 50.0%
!! AGGGAAGTAAGCATTGGAGG+AGG - chr4_1:16665275-16665294 Msa0537760:CDS 50.0%
!! GGGAAGTAAGCATTGGAGGA+GGG - chr4_1:16665276-16665295 Msa0537760:CDS 50.0%
ATCTTGGGAGAGACAACTCG+AGG - chr4_1:16665468-16665487 Msa0537760:CDS 50.0%
GAGAATGGAGTTGGCAATGG+AGG + chr4_1:16663929-16663948 None:intergenic 50.0%
ATACACACGTGCTGGACCTT+CGG - chr4_1:16664360-16664379 Msa0537760:CDS 50.0%
TTGCCTCAAAGCATGGCCAA+TGG - chr4_1:16664676-16664695 Msa0537760:intron 50.0%
GATCCAACATGCATGGCCAT+TGG + chr4_1:16664695-16664714 None:intergenic 50.0%
TTTGACCGAAAGGGCTCCAT+TGG - chr4_1:16665015-16665034 Msa0537760:intron 50.0%
!!! GTGGCTGCTTTTTATCTGGC+AGG - chr4_1:16665108-16665127 Msa0537760:intron 50.0%
!! GAGAGGGAAGTAAGCATTGG+AGG - chr4_1:16665272-16665291 Msa0537760:CDS 50.0%
!! AGGGAAGTAAGCATTGGAGG+AGG - chr4_1:16665275-16665294 Msa0537760:CDS 50.0%
!! GGGAAGTAAGCATTGGAGGA+GGG - chr4_1:16665276-16665295 Msa0537760:CDS 50.0%
ATCTTGGGAGAGACAACTCG+AGG - chr4_1:16665468-16665487 Msa0537760:CDS 50.0%
TGGCCATTGGCCATGCTTTG+AGG + chr4_1:16664682-16664701 None:intergenic 55.0%
TGGCCAATGGCCATGCATGT+TGG - chr4_1:16664689-16664708 Msa0537760:intron 55.0%
!! GAGGAGGGTACCACATTGAG+AGG - chr4_1:16665291-16665310 Msa0537760:CDS 55.0%
TGGCCATTGGCCATGCTTTG+AGG + chr4_1:16664682-16664701 None:intergenic 55.0%
TGGCCAATGGCCATGCATGT+TGG - chr4_1:16664689-16664708 Msa0537760:intron 55.0%
!! GAGGAGGGTACCACATTGAG+AGG - chr4_1:16665291-16665310 Msa0537760:CDS 55.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 16663883 16665794 16663883 ID=Msa0537760;Name=Msa0537760
chr4_1 mRNA 16663883 16665794 16663883 ID=Msa0537760-mRNA-1;Parent=Msa0537760;Name=Msa0537760-mRNA-1;_AED=0.01;_eAED=0.01;_QI=223|1|1|1|1|1|2|415|218
chr4_1 exon 16663883 16664547 16663883 ID=Msa0537760-mRNA-1:exon:4957;Parent=Msa0537760-mRNA-1
chr4_1 exon 16665165 16665794 16665165 ID=Msa0537760-mRNA-1:exon:4956;Parent=Msa0537760-mRNA-1
chr4_1 five_prime_UTR 16665572 16665794 16665572 ID=Msa0537760-mRNA-1:five_prime_utr;Parent=Msa0537760-mRNA-1
chr4_1 CDS 16665165 16665571 16665165 ID=Msa0537760-mRNA-1:cds;Parent=Msa0537760-mRNA-1
chr4_1 CDS 16664298 16664547 16664298 ID=Msa0537760-mRNA-1:cds;Parent=Msa0537760-mRNA-1
chr4_1 three_prime_UTR 16663883 16664297 16663883 ID=Msa0537760-mRNA-1:three_prime_utr;Parent=Msa0537760-mRNA-1
Gene Sequence

>Msa0537760

ATGGATTCTTCTTCTTTGACAATATCTCCAAGAAAACTACACTATGATTTATATTCCTATTCATACAAAGAAGATTCAAACACCCCATTAGTGATAAATGTTCTAGCTTCATTAATTGAGAGAAACATGGCAAGAGCACAAAGAATTGTGAAGAATTGTTCTTCAAGAGTTTTGTCCAAAGCAAGCACAAAAATCTTTGATTGTAGAGAGATTCCTGATTTAACCATTCAATCTTATTTAGAGAGAATTTTTAGATACACACGTGCTGGACCTTCGGTTTATGTTGTTGCTTATGTCTATATTGATCGGTTTTGCCAAAATAATCTTGGGTTTAGAATTAATTCAAGAAATGTTCATAGACTTCTCATAACAACTATTATGGTTGCTTCAAAGTATGTTGAAGATATGAATTTCAGAAATTCATACTTTGCAAAAGTTGGTGGTTTAACAACAAATGAATTGAATGAGTTGGAGCTTGAATTTCTTTTCATGATGAATTTCAAATTACATGTTAATGTGAGTGTATTTGAGAGCTATTGTTCCCATTTGGAGAGGGAAGTAAGCATTGGAGGAGGGTACCACATTGAGAGGACCTTAAGGTGTGCTGAAGAAATCAAAGAAAGAGGTCACACACAAATTGCACGTGTAATGTTGTAG
Protein sequence

>Msa0537760

MDSSSLTISPRKLHYDLYSYSYKEDSNTPLVINVLASLIERNMARAQRIVKNCSSRVLSKASTKIFDCREIPDLTIQSYLERIFRYTRAGPSVYVVAYVYIDRFCQNNLGFRINSRNVHRLLITTIMVASKYVEDMNFRNSYFAKVGGLTTNELNELELEFLFMMNFKLHVNVSVFESYCSHLEREVSIGGGYHIERTLRCAEEIKERGHTQIARVML*