AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0618640

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0618640 MtrunA17_Chr8g0379761 75.145 173 41 2 7 178 28 199 6.69e-90 262
Msa0618640 MtrunA17_Chr8g0379811 71.429 175 48 2 7 179 12 186 3.04e-87 254
Msa0618640 MtrunA17_Chr8g0379741 78.235 170 32 4 11 178 199 365 1.73e-85 256
Msa0618640 MtrunA17_Chr8g0379741 72.973 111 29 1 60 170 8 117 9.36e-52 170
Msa0618640 MtrunA17_Chr8g0379801 70.286 175 49 2 4 178 146 317 7.52e-81 243
Msa0618640 MtrunA17_Chr8g0379781 69.364 173 50 2 6 178 23 192 1.82e-80 238
Msa0618640 MtrunA17_Chr8g0379711 69.274 179 49 4 1 178 10 183 2.11e-79 235
Msa0618640 MtrunA17_Chr8g0379721 64.000 175 44 3 4 178 92 247 2.98e-67 206
Msa0618640 MtrunA17_Chr3g0081941 33.571 140 73 3 36 174 164 284 1.69e-17 80.1
Msa0618640 MtrunA17_Chr2g0313461 33.071 127 66 2 51 177 189 296 7.19e-16 75.1
Msa0618640 MtrunA17_Chr2g0313591 32.283 127 67 2 51 177 189 296 1.19e-15 74.7
Msa0618640 MtrunA17_Chr2g0314301 31.496 127 68 2 51 177 187 294 1.83e-15 73.9
Msa0618640 MtrunA17_Chr2g0313491 32.576 132 70 2 51 182 189 301 6.03e-15 72.4
Msa0618640 MtrunA17_Chr1g0163981 34.400 125 63 2 51 175 189 294 2.57e-14 70.5
Msa0618640 MtrunA17_Chr2g0313541 33.071 127 66 3 51 177 189 296 3.18e-14 70.5
Msa0618640 MtrunA17_Chr6g0474571 34.400 125 63 2 51 175 189 294 5.99e-14 69.7
Msa0618640 MtrunA17_Chr1g0172271 36.585 123 59 4 51 173 190 293 1.03e-13 68.9
Msa0618640 MtrunA17_Chr2g0313281 31.452 124 66 2 51 174 189 293 1.16e-13 68.6
Msa0618640 MtrunA17_Chr1g0167981 34.146 123 62 2 51 173 190 293 1.17e-13 68.9
Msa0618640 MtrunA17_Chr2g0313481 31.496 127 68 2 51 177 189 296 1.29e-13 68.6
Msa0618640 MtrunA17_Chr1g0164011 32.800 125 65 2 51 175 190 295 2.06e-13 68.2
Msa0618640 MtrunA17_Chr2g0313321 31.373 153 83 4 23 174 162 293 2.48e-13 67.0
Msa0618640 MtrunA17_Chr1g0163831 30.323 155 86 4 20 173 159 292 2.67e-13 67.4
Msa0618640 MtrunA17_Chr1g0172051 30.323 155 86 4 20 173 159 292 2.67e-13 67.8
Msa0618640 MtrunA17_Chr1g0172141 30.323 155 86 4 20 173 159 292 4.45e-13 67.0
Msa0618640 MtrunA17_Chr2g0313351 31.250 128 69 3 51 178 193 301 5.12e-13 67.0
Msa0618640 MtrunA17_Chr2g0313391 31.707 123 65 3 51 173 193 296 5.24e-13 66.6
Msa0618640 MtrunA17_Chr2g0313401 42.857 63 36 0 120 182 11 73 5.67e-13 63.5
Msa0618640 MtrunA17_Chr1g0163791 32.117 137 72 3 37 173 177 292 7.89e-13 66.2
Msa0618640 MtrunA17_Chr1g0174511 32.117 137 72 3 37 173 177 292 8.43e-13 66.2
Msa0618640 MtrunA17_Chr1g0163861 31.447 159 83 5 18 173 158 293 1.56e-12 65.5
Msa0618640 MtrunA17_Chr1g0172111 32.520 123 63 3 51 173 189 291 1.63e-12 65.5
Msa0618640 MtrunA17_Chr1g0163761 30.573 157 87 4 18 173 158 293 1.66e-12 65.5
Msa0618640 MtrunA17_Chr2g0313431 30.709 127 69 2 51 177 189 296 1.94e-12 65.1
Msa0618640 MtrunA17_Chr2g0313311 31.250 128 69 3 51 178 189 297 1.95e-12 65.1
Msa0618640 MtrunA17_Chr2g0313521 31.967 122 64 3 57 178 199 301 2.06e-12 65.1
Msa0618640 MtrunA17_Chr1g0163891 29.487 156 89 3 18 173 157 291 2.33e-12 65.1
Msa0618640 MtrunA17_Chr1g0163931 31.447 159 83 5 18 173 158 293 2.56e-12 65.1
Msa0618640 MtrunA17_Chr3g0108821 27.933 179 98 6 7 175 131 288 2.93e-12 64.7
Msa0618640 MtrunA17_Chr1g0172171 32.520 123 63 3 51 173 189 291 3.07e-12 64.3
Msa0618640 MtrunA17_Chr3g0107931 27.742 155 90 4 22 175 178 311 4.67e-12 64.3
Msa0618640 MtrunA17_Chr3g0097031 27.933 179 98 6 7 175 80 237 7.07e-12 63.5
Msa0618640 MtrunA17_Chr7g0223931 30.252 119 64 2 52 170 11 110 8.75e-12 63.2
Msa0618640 MtrunA17_Chr2g0323691 29.032 155 88 4 20 173 159 292 8.90e-12 63.5
Msa0618640 MtrunA17_Chr8g0359881 31.707 123 65 3 51 173 189 292 8.94e-12 63.2
Msa0618640 MtrunA17_Chr1g0170881 29.936 157 88 4 18 173 157 292 9.49e-12 63.2
Msa0618640 MtrunA17_Chr3g0108811 26.437 174 97 6 7 170 143 295 3.09e-11 61.6
Msa0618640 MtrunA17_Chr6g0475721 30.769 117 62 2 51 167 189 286 3.31e-11 61.6
Msa0618640 MtrunA17_Chr1g0163631 31.126 151 78 5 18 165 157 284 4.89e-11 60.5
Msa0618640 MtrunA17_Chr3g0091851 28.333 120 67 2 51 170 124 224 6.76e-11 60.8
Msa0618640 MtrunA17_Chr3g0105521 27.746 173 102 3 5 175 21 172 7.29e-11 60.8
Msa0618640 MtrunA17_Chr3g0108211 28.333 120 67 2 51 170 10 110 7.79e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 158 sgRNAs with CRISPR-Local

Find 316 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACCTTCCTCTCTAATAAAA+TGG 0.167514 4_2:-69569607 Msa0618640:three_prime_UTR
TCTATTATGCTGTTGATTAT+AGG 0.172374 4_2:-69568168 Msa0618640:three_prime_UTR
AAATGGAAGATTGGGGATTC+AGG 0.176659 4_2:+69570512 None:intergenic
TTTCCTCTCTGTTTCTTTAA+AGG 0.192316 4_2:-69569642 Msa0618640:three_prime_UTR
CTTAGGCCATTATGTGCTTT+TGG 0.204462 4_2:-69568030 Msa0618640:three_prime_UTR
ATGGGGAAAGTTCAAATTTC+AGG 0.213329 4_2:+69568095 None:intergenic
CGGCAATCCGGGAACAGTTT+GGG 0.217269 4_2:-69570426 Msa0618640:three_prime_UTR
ACAAAGATTCAAACACTAAT+TGG 0.231039 4_2:-69573293 Msa0618640:CDS
ATGTCACAGAAAGAAAATTT+TGG 0.247378 4_2:+69570634 None:intergenic
GACCAAGGAGAAAATATAAT+TGG 0.266280 4_2:-69570336 Msa0618640:three_prime_UTR
ACAAAGATTCAAACACAAAT+TGG 0.268354 4_2:-69570195 Msa0618640:three_prime_UTR
AATGGAGACAAAGGCTAGTT+TGG 0.272742 4_2:-69567990 Msa0618640:three_prime_UTR
TTGTAGCTTTGTAACTTCTT+AGG 0.273848 4_2:-69568047 Msa0618640:three_prime_UTR
CAATTATACAATTATTTATC+AGG 0.276393 4_2:+69569446 None:intergenic
TAGAAGTTGATTAAAAGATA+TGG 0.277039 4_2:+69568187 None:intergenic
TTCCTCTCTGTTTCTTTAAA+GGG 0.278436 4_2:-69569641 Msa0618640:three_prime_UTR
TTGGGTTACGCTTACATTCT+TGG 0.285181 4_2:-69567971 Msa0618640:three_prime_UTR
AAACTACTTGATTGTCTGTT+TGG 0.286371 4_2:-69568901 Msa0618640:three_prime_UTR
TTTATCAACCTTCATATGTT+TGG 0.289537 4_2:+69567504 None:intergenic
GTTGTAGTGTATGATCTTTA+TGG 0.289807 4_2:-69573125 Msa0618640:CDS
ACATTCTTGGCATGGATGAT+AGG 0.296319 4_2:-69567958 Msa0618640:three_prime_UTR
TTTACATTCCTAACGATGAT+AGG 0.297335 4_2:-69573496 Msa0618640:CDS
GTTTGAATCTTTGTAATATC+TGG 0.300279 4_2:+69570204 None:intergenic
CAGTTGCTGTGACAATCTTT+TGG 0.304172 4_2:+69570238 None:intergenic
GCTCACATCTACAAGCTTAG+AGG 0.306403 4_2:-69567533 Msa0618640:three_prime_UTR
GTTATGAACCTTCATTTGTT+TGG 0.307325 4_2:+69573010 None:intergenic
GGAAGCCTGCGTTTCGTCTA+TGG 0.320856 4_2:-69570935 Msa0618640:CDS
TCGGCAATCCGGGAACAGTT+TGG 0.321768 4_2:-69570427 Msa0618640:three_prime_UTR
ACGAAGAATGATAGCTTGTT+TGG 0.326372 4_2:+69569173 None:intergenic
AACTCATTCAATCGTTTGAT+AGG 0.329712 4_2:+69570754 None:intergenic
GAAAAGAGAAATGGAAGATT+GGG 0.331098 4_2:+69570504 None:intergenic
AATATGTGTCAACATATAGA+TGG 0.333282 4_2:+69569283 None:intergenic
TCCGAGACATGTATCCATTA+TGG 0.336897 4_2:-69569692 Msa0618640:three_prime_UTR
GAGGTTGAGAAGTGCTTTGC+AGG 0.338050 4_2:-69569767 Msa0618640:three_prime_UTR
ATTGCTCACATCTACAAGTT+TGG 0.339784 4_2:-69569944 Msa0618640:three_prime_UTR
ATTGCTCACATCTACAAGTT+TGG 0.339784 4_2:-69573042 Msa0618640:CDS
TCCAAACGATGTGTATGTTT+GGG 0.345057 4_2:+69569812 None:intergenic
CATTAGACTCCACGTTTGTT+TGG 0.350063 4_2:+69568627 None:intergenic
AGGCTTTCTAGCTTGATGTT+TGG 0.353771 4_2:-69568148 Msa0618640:three_prime_UTR
CATTATGTCGTTTGAGAGAT+TGG 0.354751 4_2:-69570451 Msa0618640:three_prime_UTR
TTCCAAACGATGTGTATGTT+TGG 0.356364 4_2:+69569811 None:intergenic
AACCAAGGAGAAAATTTAAT+CGG 0.360137 4_2:-69573437 Msa0618640:CDS
GACCTATTGCATCACCAATT+AGG 0.366059 4_2:+69567773 None:intergenic
CTGTGTCAAAGATGCCTAAT+TGG 0.368817 4_2:-69567787 Msa0618640:three_prime_UTR
TTGTTGTTGTGACAATCTTT+TGG 0.375012 4_2:+69573339 None:intergenic
GTTGTACTGTATGATCTTCA+TGG 0.377791 4_2:-69570027 Msa0618640:three_prime_UTR
AGACTTGATTTAAGTAAGAT+TGG 0.378585 4_2:-69573101 Msa0618640:CDS
CAAGGGAAATCTTACCATAA+TGG 0.379067 4_2:+69569678 None:intergenic
CTAATAAAATGGTTTGAACT+AGG 0.388506 4_2:-69569596 Msa0618640:three_prime_UTR
GCCCAAACATACACATCGTT+TGG 0.393400 4_2:-69569813 Msa0618640:three_prime_UTR
TGATAGCTTGTTTGGTTGAA+AGG 0.394849 4_2:+69569181 None:intergenic
ATTGTACTGTATGATCTTCA+TGG 0.395468 4_2:-69567619 Msa0618640:three_prime_UTR
GAATATTAACTTGGAGTGTA+AGG 0.397176 4_2:-69569480 Msa0618640:three_prime_UTR
TTCAATCGTTTGATAGGAAA+AGG 0.399907 4_2:+69570760 None:intergenic
AGTAAGATTGGTATCCCCTT+TGG 0.401473 4_2:-69573089 Msa0618640:CDS
TGAAAAGAGAAATGGAAGAT+TGG 0.402189 4_2:+69570503 None:intergenic
TCAACTTGTTGCATGTGTTT+TGG 0.403016 4_2:-69567718 Msa0618640:three_prime_UTR
TAAGCCTTGACTCTTCTATC+TGG 0.406379 4_2:+69573404 None:intergenic
CAATCTCTCAAACGACATAA+TGG 0.407214 4_2:+69570452 None:intergenic
CTAGTCTATAGCGTCTCGTT+TGG 0.409998 4_2:+69568728 None:intergenic
CACCATGTTGGAGAGATTAG+AGG 0.412312 4_2:-69569786 Msa0618640:three_prime_UTR
GACCAAGGAGAAAATATAAT+CGG 0.412405 4_2:-69567905 Msa0618640:three_prime_UTR
CCCTTTGGTGAAGATCATAA+TGG 0.415861 4_2:-69569976 Msa0618640:three_prime_UTR
CCCTTTGGTGAAGATCATAA+TGG 0.415861 4_2:-69573074 Msa0618640:CDS
AGTGAGATTGGTATCCCCTT+TGG 0.416147 4_2:-69569991 Msa0618640:three_prime_UTR
TCTGCTACAAACGCTCTTGT+TGG 0.417044 4_2:+69569853 None:intergenic
ACACTAATTGGTGATGCAAT+AGG 0.421541 4_2:-69573281 Msa0618640:CDS
TATTCCCAGCGTGGATGATA+AGG 0.422594 4_2:-69570394 Msa0618640:three_prime_UTR
GGTTTGAACTAGGTTTGATT+TGG 0.424336 4_2:-69569586 Msa0618640:three_prime_UTR
TAATTTAGTAGTCTGTTTGA+TGG 0.425089 4_2:-69568234 Msa0618640:three_prime_UTR
ACTTTCCCCATTGATTTAAG+TGG 0.425177 4_2:-69568083 Msa0618640:three_prime_UTR
ACACAAATTGGTGATGCAAT+AGG 0.433183 4_2:-69570183 Msa0618640:three_prime_UTR
GGAGAGATTGGTATCCCCTT+TGG 0.438019 4_2:-69567583 Msa0618640:three_prime_UTR
GTTGATAACAGATATGTTAA+CGG 0.440028 4_2:-69569044 Msa0618640:three_prime_UTR
GAGAGATTGGTTCGGCAATC+CGG 0.440927 4_2:-69570438 Msa0618640:three_prime_UTR
TGAACTAGGTTTGATTTGGT+TGG 0.441398 4_2:-69569582 Msa0618640:three_prime_UTR
GGCAATCCGGGAACAGTTTG+GGG 0.442842 4_2:-69570425 Msa0618640:three_prime_UTR
AGAAGTTGATTAAAAGATAT+GGG 0.445916 4_2:+69568188 None:intergenic
GATGCGGAGGAGGAGGAGAA+TGG 0.449101 4_2:-69568008 Msa0618640:three_prime_UTR
CAATTACATAATTAGTTATG+AGG 0.451628 4_2:+69568308 None:intergenic
CATTCTTGGCATGGATGATA+GGG 0.451948 4_2:-69567957 Msa0618640:three_prime_UTR
AGAGATTGGTTCGGCAATCC+GGG 0.458252 4_2:-69570437 Msa0618640:three_prime_UTR
CAGATTATGGAAGATATTGC+AGG 0.458455 4_2:-69573536 None:intergenic
AATGGAAGATTGGGGATTCA+GGG 0.458757 4_2:+69570513 None:intergenic
ATGGGTTATGTGAGTAACTT+TGG 0.459188 4_2:+69569302 None:intergenic
AGACTCGATTTAAGTGAGAT+TGG 0.459620 4_2:-69570003 Msa0618640:three_prime_UTR
TCTCTCAGCTCTTCTTATAA+CGG 0.460444 4_2:+69573373 None:intergenic
CCGCATCCAAAAGCACATAA+TGG 0.461890 4_2:+69568024 None:intergenic
ATGGAGACAAAGGCTAGTTT+GGG 0.462080 4_2:-69567989 Msa0618640:three_prime_UTR
AGAAGAGGAAATAAACTTGC+TGG 0.465394 4_2:+69568409 None:intergenic
CTCTCTGTTTCTTTAAAGGG+AGG 0.469502 4_2:-69569638 Msa0618640:three_prime_UTR
AATTTAGTAGTCTGTTTGAT+GGG 0.471421 4_2:-69568233 Msa0618640:three_prime_UTR
AACTAATTATGTAATTGTAC+TGG 0.472288 4_2:-69568302 Msa0618640:three_prime_UTR
TTCTTAACCCTATCATCGTT+AGG 0.474445 4_2:+69573488 None:intergenic
ATCATCTAAAATATAATGAA+TGG 0.477395 4_2:+69569339 None:intergenic
TTGCAGGTATAGATGAACTA+AGG 0.480270 4_2:-69569751 Msa0618640:three_prime_UTR
TGCCTAATTGGTGATGCAAT+AGG 0.493176 4_2:-69567775 Msa0618640:three_prime_UTR
GCTCACATCTACAAGTTTGG+AGG 0.498220 4_2:-69569941 Msa0618640:three_prime_UTR
AGACTCGATCTAGGAGAGAT+TGG 0.498258 4_2:-69567595 Msa0618640:three_prime_UTR
TCATTATGATCTTCACCAAA+GGG 0.503786 4_2:+69567568 None:intergenic
TTTATGAACCTTCATTTGCT+CGG 0.509437 4_2:+69569912 None:intergenic
TCTCTGTTTCTTTAAAGGGA+GGG 0.515301 4_2:-69569637 Msa0618640:three_prime_UTR
TCGTTAGGAATGTAAATGTC+CGG 0.516128 4_2:+69573503 None:intergenic
GAAGATCATACAGTACAATC+AGG 0.516562 4_2:+69567623 None:intergenic
AGAAGCTTATATAATTGAAG+TGG 0.518795 4_2:+69568451 None:intergenic
AAAATATAATTGGATTATGC+GGG 0.519176 4_2:-69570326 Msa0618640:three_prime_UTR
GAAAATTTAATCGGATTATG+TGG 0.519822 4_2:-69573428 Msa0618640:CDS
CTTCATGGTAGACTCGATCT+AGG 0.520000 4_2:-69567604 Msa0618640:three_prime_UTR
ATTCTGTCAAATTCTTGTGT+TGG 0.522510 4_2:-69569722 Msa0618640:three_prime_UTR
CCATTATGATCTTCACCAAA+GGG 0.522614 4_2:+69569976 None:intergenic
CCATTATGATCTTCACCAAA+GGG 0.522614 4_2:+69573074 None:intergenic
TTACGCTTACATTCTTGGCA+TGG 0.525082 4_2:-69567966 Msa0618640:three_prime_UTR
TTACATTCCTAACGATGATA+GGG 0.527253 4_2:-69573495 Msa0618640:CDS
GAAAATATAATTGGATTATG+CGG 0.532748 4_2:-69570327 Msa0618640:three_prime_UTR
AAATAATTGTATAATTGCAA+AGG 0.538610 4_2:-69569440 Msa0618640:three_prime_UTR
ACCATAATGGATACATGTCT+CGG 0.548726 4_2:+69569691 None:intergenic
GGAAATTACTGCAAGAAACA+AGG 0.549652 4_2:+69569660 None:intergenic
TGTCGTTTGAGAGATTGGTT+CGG 0.553620 4_2:-69570446 Msa0618640:three_prime_UTR
CGAACTCTTACAACAACTCA+AGG 0.559380 4_2:+69568541 None:intergenic
CTGTTTCTTTAAAGGGAGGG+AGG 0.559892 4_2:-69569634 Msa0618640:three_prime_UTR
AAAATATAATCGGATTATGC+GGG 0.561138 4_2:-69567895 Msa0618640:three_prime_UTR
ATATGTGTCAACATATAGAT+GGG 0.562099 4_2:+69569284 None:intergenic
AGACGAAACGCAGGCTTCCG+TGG 0.564967 4_2:+69570939 None:intergenic
ATCATTATGATCTTCACCAA+AGG 0.565394 4_2:+69567567 None:intergenic
GGGAGAGAAGAAGATTAAGA+TGG 0.572937 4_2:+69570533 None:intergenic
GGTGCTGAATAAAGAAGAAG+AGG 0.577169 4_2:+69568493 None:intergenic
ATGCAACAAGTTGATTCAGC+CGG 0.577268 4_2:+69570135 None:intergenic
ATTATAAAACTTGTATCCCA+AGG 0.577280 4_2:+69568253 None:intergenic
TTAACCTTATCATCCACGCT+GGG 0.579493 4_2:+69570390 None:intergenic
GCAATCCGGGAACAGTTTGG+GGG 0.584978 4_2:-69570424 Msa0618640:three_prime_UTR
GAAAATATAATCGGATTATG+CGG 0.588031 4_2:-69567896 Msa0618640:three_prime_UTR
TTGATTAAAAGATATGGGAA+AGG 0.590309 4_2:+69568193 None:intergenic
ACCATTATGATCTTCACCAA+AGG 0.590609 4_2:+69569975 None:intergenic
ACCATTATGATCTTCACCAA+AGG 0.590609 4_2:+69573073 None:intergenic
GCACTAAGAGTGAGAGACCA+AGG 0.594788 4_2:-69570351 Msa0618640:three_prime_UTR
GAAATTACTGCAAGAAACAA+GGG 0.595841 4_2:+69569661 None:intergenic
GAGGAGGAGAATGGAGACAA+AGG 0.596331 4_2:-69567999 Msa0618640:three_prime_UTR
AGATTATGGAAGATATTGCA+GGG 0.603328 4_2:-69573535 None:intergenic
GCACTAAGAGTGAGTAACCA+AGG 0.605228 4_2:-69573452 Msa0618640:CDS
AAAAGAGAAATGGAAGATTG+GGG 0.606123 4_2:+69570505 None:intergenic
ACATATCTGTTATCAACTGT+AGG 0.606947 4_2:+69569050 None:intergenic
TCTGAACTTTGATGATAAGA+CGG 0.612552 4_2:-69567752 Msa0618640:three_prime_UTR
TCGTTTGGAAAACACCATGT+TGG 0.627259 4_2:-69569798 Msa0618640:three_prime_UTR
TGGTCCAGATAGAAGAGTCA+AGG 0.629291 4_2:-69573408 Msa0618640:CDS
GCTTTATGAAAGAAAGACCA+AGG 0.634593 4_2:-69567920 Msa0618640:three_prime_UTR
TTTAACCTTATCATCCACGC+TGG 0.639042 4_2:+69570389 None:intergenic
AGCCTGCGTTTCGTCTATGG+TGG 0.641036 4_2:-69570932 Msa0618640:CDS
GAAGATCATACAGTACAACT+AGG 0.653715 4_2:+69570031 None:intergenic
CACCACCATAGACGAAACGC+AGG 0.663946 4_2:+69570930 None:intergenic
AAAGATCATACACTACAACT+AGG 0.664670 4_2:+69573129 None:intergenic
GATTATGGAAGATATTGCAG+GGG 0.676733 4_2:-69573534 None:intergenic
GGGCATTTATATTCCCAGCG+TGG 0.677027 4_2:-69570403 Msa0618640:three_prime_UTR
CATTATGATCTTCACCAAAG+GGG 0.682494 4_2:+69567569 None:intergenic
CAATCCGGGAACAGTTTGGG+GGG 0.685818 4_2:-69570423 Msa0618640:three_prime_UTR
AACCTCTAATCTCTCCAACA+TGG 0.687446 4_2:+69569784 None:intergenic
CATTATGATCTTCACCAAAG+GGG 0.695532 4_2:+69569977 None:intergenic
CATTATGATCTTCACCAAAG+GGG 0.695532 4_2:+69573075 None:intergenic
CTCCCTTTAAAGAAACAGAG+AGG 0.703957 4_2:+69569639 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAAAAAAAAATAAAAAATT+TGG + chr4_2:69570346-69570365 None:intergenic 0.0%
!! TTAAAAAAACAAGATTAAAT+AGG - chr4_2:69568658-69568677 Msa0618640:three_prime_UTR 10.0%
!!! CATTTTTTTATTATTAAAAG+AGG - chr4_2:69568940-69568959 Msa0618640:three_prime_UTR 10.0%
!! TATTTAACTAAAAAATGATT+TGG - chr4_2:69569229-69569248 Msa0618640:three_prime_UTR 10.0%
!! AAAAAAAATAAAAAATTTGG+AGG + chr4_2:69570343-69570362 None:intergenic 10.0%
!! TGATAAATACATTTGATAAA+AGG + chr4_2:69568345-69568364 None:intergenic 15.0%
!!! TTTTACTTATGAATCTTTTT+TGG - chr4_2:69568386-69568405 Msa0618640:three_prime_UTR 15.0%
!! ATGTTTCCTTAAAAATTTAA+CGG + chr4_2:69568448-69568467 None:intergenic 15.0%
!!! TAAATTTTTAAGGAAACATA+TGG - chr4_2:69568449-69568468 Msa0618640:three_prime_UTR 15.0%
!!! ATTCATTATTTTCTTTTACA+GGG + chr4_2:69568992-69569011 None:intergenic 15.0%
!!! TATTCATTATTTTCTTTTAC+AGG + chr4_2:69568993-69569012 None:intergenic 15.0%
!!! TCTTAATTTATGTTTTGATA+CGG + chr4_2:69569121-69569140 None:intergenic 15.0%
!! AAAAAATGCATTATAAACAT+AGG + chr4_2:69569508-69569527 None:intergenic 15.0%
!!! ATTTTTTATCTTCTAGTTAA+AGG - chr4_2:69569521-69569540 Msa0618640:three_prime_UTR 15.0%
!! CAAAGTTTCATTTAAAAATT+TGG - chr4_2:69569737-69569756 Msa0618640:three_prime_UTR 15.0%
!!! TAGATTTTTGAATATTAACT+TGG - chr4_2:69571528-69571547 Msa0618640:intron 15.0%
!! CAATTATACAATTATTTATC+AGG + chr4_2:69571574-69571593 None:intergenic 15.0%
!! AAATAATTGTATAATTGCAA+AGG - chr4_2:69571577-69571596 Msa0618640:intron 15.0%
!!! ATCATCTAAAATATAATGAA+TGG + chr4_2:69571681-69571700 None:intergenic 15.0%
!!! ATTTTTCTATAAATATTCCT+TGG - chr4_2:69572747-69572766 Msa0618640:intron 15.0%
!!! TTTTTCTATAAATATTCCTT+GGG - chr4_2:69572748-69572767 Msa0618640:intron 15.0%
!! ATAGTTATTATTATCAGAAC+TGG + chr4_2:69568188-69568207 None:intergenic 20.0%
!! ACAAGTTAAAATTTGACAAA+AGG + chr4_2:69568232-69568251 None:intergenic 20.0%
!!! GATGTAAAAACAATTTTCAT+TGG - chr4_2:69568534-69568553 Msa0618640:three_prime_UTR 20.0%
!!! GTAATTTGCATTTAATTTCA+TGG - chr4_2:69568598-69568617 Msa0618640:three_prime_UTR 20.0%
!!! CAATAAACTAAAGTTTTTCT+AGG - chr4_2:69568621-69568640 Msa0618640:three_prime_UTR 20.0%
!!! AGTTTTTTTCCTTACATTTT+GGG - chr4_2:69568726-69568745 Msa0618640:three_prime_UTR 20.0%
!! TTTCATATAAAATATCCTCA+CGG + chr4_2:69568799-69568818 None:intergenic 20.0%
!!! TTCATTATTTTCTTTTACAG+GGG + chr4_2:69568991-69569010 None:intergenic 20.0%
!! TGTATCTTAATAAATGATGT+TGG + chr4_2:69569058-69569077 None:intergenic 20.0%
!!! GGCTGTTAAATTATTTTTTA+AGG - chr4_2:69569618-69569637 Msa0618640:three_prime_UTR 20.0%
!!! ATTATTTTTTAAGGATGAGT+AGG - chr4_2:69569627-69569646 Msa0618640:three_prime_UTR 20.0%
!! TAAGTAATTGTGTAAATGTA+AGG - chr4_2:69569797-69569816 Msa0618640:three_prime_UTR 20.0%
!!! TTATAACATAGTAGTCTTTT+TGG - chr4_2:69569883-69569902 Msa0618640:three_prime_UTR 20.0%
!! TGTAAAATTTCTATTTCAAG+TGG - chr4_2:69569997-69570016 Msa0618640:three_prime_UTR 20.0%
!!! CGCAATAGAAATATTTTTAA+AGG + chr4_2:69570234-69570253 None:intergenic 20.0%
!!! TTTTTTGATGAAAAGAGAAA+TGG + chr4_2:69570525-69570544 None:intergenic 20.0%
!! GAAAATATAATTGGATTATG+CGG - chr4_2:69570690-69570709 Msa0618640:three_prime_UTR 20.0%
!! AAAATATAATTGGATTATGC+GGG - chr4_2:69570691-69570710 Msa0618640:three_prime_UTR 20.0%
!!! TCTGAATTTTGATGATAAAA+CGG - chr4_2:69570857-69570876 Msa0618640:three_prime_UTR 20.0%
!!! TTTTGGTAATAGAAAAAGAA+TGG - chr4_2:69570908-69570927 Msa0618640:exon 20.0%
!! AAAAACTTTAGTCATTTGTT+TGG - chr4_2:69571873-69571892 Msa0618640:intron 20.0%
!!! TTTTTTAATTAAGACTGCAA+TGG - chr4_2:69572064-69572083 Msa0618640:intron 20.0%
!! GTATAGTTTATGAAAAAAAC+TGG - chr4_2:69572218-69572237 Msa0618640:intron 20.0%
!! TTTGACTAAAAAATTGTCTT+AGG + chr4_2:69572327-69572346 None:intergenic 20.0%
!!! GGTTTATTTTTTGTGTATAT+GGG + chr4_2:69572548-69572567 None:intergenic 20.0%
!!! TGGTTTATTTTTTGTGTATA+TGG + chr4_2:69572549-69572568 None:intergenic 20.0%
!!! TTGATTTTCACTGTATAATA+AGG - chr4_2:69572667-69572686 Msa0618640:intron 20.0%
!! CAATTACATAATTAGTTATG+AGG + chr4_2:69572712-69572731 None:intergenic 20.0%
!! AACTAATTATGTAATTGTAC+TGG - chr4_2:69572715-69572734 Msa0618640:intron 20.0%
!!! AGAAGTTGATTAAAAGATAT+GGG + chr4_2:69572832-69572851 None:intergenic 20.0%
!!! TAGAAGTTGATTAAAAGATA+TGG + chr4_2:69572833-69572852 None:intergenic 20.0%
!!! TTTTTTCCACTTAAATCAAT+GGG + chr4_2:69572943-69572962 None:intergenic 20.0%
!!! TTTTTTTCCACTTAAATCAA+TGG + chr4_2:69572944-69572963 None:intergenic 20.0%
! AACCAAGGAGAAAATTTAAT+CGG - chr4_2:69567580-69567599 Msa0618640:three_prime_UTR 25.0%
! GAAAATTTAATCGGATTATG+TGG - chr4_2:69567589-69567608 Msa0618640:three_prime_UTR 25.0%
! ACAAAGATTCAAACACTAAT+TGG - chr4_2:69567724-69567743 Msa0618640:three_prime_UTR 25.0%
! AGACTTGATTTAAGTAAGAT+TGG - chr4_2:69567916-69567935 Msa0618640:three_prime_UTR 25.0%
!! GATTTTATCCAAACAAATGA+AGG - chr4_2:69567999-69568018 Msa0618640:three_prime_UTR 25.0%
!!! ATGTTTGATAAGTGCTTTTT+AGG - chr4_2:69568101-69568120 Msa0618640:three_prime_UTR 25.0%
!!! TTTAACTTGTTTTTGCCATA+TGG - chr4_2:69568242-69568261 Msa0618640:three_prime_UTR 25.0%
!!! TTAACTTGTTTTTGCCATAT+GGG - chr4_2:69568243-69568262 Msa0618640:three_prime_UTR 25.0%
! AACACTAAAGATCACATAAA+TGG + chr4_2:69568322-69568341 None:intergenic 25.0%
!! GTTTGACCGTTAAATTTTTA+AGG - chr4_2:69568439-69568458 Msa0618640:three_prime_UTR 25.0%
!!! AAAGTTTTTCTAGGAAAAGA+TGG - chr4_2:69568630-69568649 Msa0618640:three_prime_UTR 25.0%
!!! GAGTTTTTTTCCTTACATTT+TGG - chr4_2:69568725-69568744 Msa0618640:three_prime_UTR 25.0%
!! TTTTATATGAAATCTGACGT+TGG - chr4_2:69568807-69568826 Msa0618640:three_prime_UTR 25.0%
! TTTCTATCTTACTATAGTGA+TGG + chr4_2:69568882-69568901 None:intergenic 25.0%
!!! TATTCTAAGATAGCATCAAT+TGG - chr4_2:69568908-69568927 Msa0618640:three_prime_UTR 25.0%
!!! ATTCTAAGATAGCATCAATT+GGG - chr4_2:69568909-69568928 Msa0618640:three_prime_UTR 25.0%
!! GTCTATCATCTAAAACATAA+TGG + chr4_2:69569950-69569969 None:intergenic 25.0%
! TAGGGAAAATTGAAATTTCA+TGG + chr4_2:69570025-69570044 None:intergenic 25.0%
!!! AAGTGTCAAACTTGTTTTTT+TGG + chr4_2:69570113-69570132 None:intergenic 25.0%
! AAATAAAAAATTTGGAGGTG+TGG + chr4_2:69570338-69570357 None:intergenic 25.0%
!! ATGTCACAGAAAGAAAATTT+TGG + chr4_2:69570386-69570405 None:intergenic 25.0%
!! ATCCAATTATATTTTCTCCT+TGG + chr4_2:69570686-69570705 None:intergenic 25.0%
! GTTTGAATCTTTGTAATATC+TGG + chr4_2:69570816-69570835 None:intergenic 25.0%
! ACAAAGATTCAAACACAAAT+TGG - chr4_2:69570822-69570841 Msa0618640:three_prime_UTR 25.0%
!! TTCAAACCATTTTATTAGAG+AGG + chr4_2:69571419-69571438 None:intergenic 25.0%
! CTAATAAAATGGTTTGAACT+AGG - chr4_2:69571421-69571440 Msa0618640:intron 25.0%
!!! CAATATTTTAGTAGTCTGTT+TGG - chr4_2:69571618-69571637 Msa0618640:intron 25.0%
! ATATGTGTCAACATATAGAT+GGG + chr4_2:69571736-69571755 None:intergenic 25.0%
! AATATGTGTCAACATATAGA+TGG + chr4_2:69571737-69571756 None:intergenic 25.0%
!!! GTTGATAACAGATATGTTAA+CGG - chr4_2:69571973-69571992 Msa0618640:intron 25.0%
! AGAAGCTTATATAATTGAAG+TGG + chr4_2:69572569-69572588 None:intergenic 25.0%
!! CTTTGAAAAAAAGAAAGAAG+AGG + chr4_2:69572626-69572645 None:intergenic 25.0%
!!! CAAAGTTTCATTTTGACTTT+AGG - chr4_2:69572641-69572660 Msa0618640:intron 25.0%
! ATTATAAAACTTGTATCCCA+AGG + chr4_2:69572767-69572786 None:intergenic 25.0%
! TAATTTAGTAGTCTGTTTGA+TGG - chr4_2:69572783-69572802 Msa0618640:intron 25.0%
! AATTTAGTAGTCTGTTTGAT+GGG - chr4_2:69572784-69572803 Msa0618640:intron 25.0%
! TTGATTAAAAGATATGGGAA+AGG + chr4_2:69572827-69572846 None:intergenic 25.0%
!! TCTATTATGCTGTTGATTAT+AGG - chr4_2:69572849-69572868 Msa0618640:intron 25.0%
!! TTTTTCCACTTAAATCAATG+GGG + chr4_2:69572942-69572961 None:intergenic 25.0%
! GAAAATATAATCGGATTATG+CGG - chr4_2:69573121-69573140 Msa0618640:CDS 25.0%
! AAAATATAATCGGATTATGC+GGG - chr4_2:69573122-69573141 Msa0618640:CDS 25.0%
! TTTATCAACCTTCATATGTT+TGG + chr4_2:69573516-69573535 None:intergenic 25.0%
TTTACATTCCTAACGATGAT+AGG - chr4_2:69567521-69567540 Msa0618640:three_prime_UTR 30.0%
TTACATTCCTAACGATGATA+GGG - chr4_2:69567522-69567541 Msa0618640:three_prime_UTR 30.0%
! ATCCGATTAAATTTTCTCCT+TGG + chr4_2:69567585-69567604 None:intergenic 30.0%
!! TTGTTGTTGTGACAATCTTT+TGG + chr4_2:69567681-69567700 None:intergenic 30.0%
AAAGATCATACACTACAACT+AGG + chr4_2:69567891-69567910 None:intergenic 30.0%
GTTGTAGTGTATGATCTTTA+TGG - chr4_2:69567892-69567911 Msa0618640:three_prime_UTR 30.0%
GTTATGAACCTTCATTTGTT+TGG + chr4_2:69568010-69568029 None:intergenic 30.0%
! TCAAACATTGTGTTTTCCAA+AGG + chr4_2:69568089-69568108 None:intergenic 30.0%
ATCACATAAATGGAGGAAAA+TGG + chr4_2:69568312-69568331 None:intergenic 30.0%
ACTAAAGATCACATAAATGG+AGG + chr4_2:69568319-69568338 None:intergenic 30.0%
! AAACAATTTTCATTGGCCAT+TGG - chr4_2:69568541-69568560 Msa0618640:three_prime_UTR 30.0%
!! TTTTTCCTTACATTTTGGGA+CGG - chr4_2:69568730-69568749 Msa0618640:three_prime_UTR 30.0%
!! CTTTATTGGTTCAAGAAGAT+GGG + chr4_2:69569274-69569293 None:intergenic 30.0%
!! TCTTTATTGGTTCAAGAAGA+TGG + chr4_2:69569275-69569294 None:intergenic 30.0%
AACCAATAAAGAGAAATGAG+TGG - chr4_2:69569283-69569302 Msa0618640:three_prime_UTR 30.0%
AAAAAGGTTGGATATTTGCT+TGG - chr4_2:69569332-69569351 Msa0618640:three_prime_UTR 30.0%
TTCACTTCACCAAATAATTG+GGG + chr4_2:69569389-69569408 None:intergenic 30.0%
CTTCACTTCACCAAATAATT+GGG + chr4_2:69569390-69569409 None:intergenic 30.0%
AGAATTGAAATCTAACCACA+AGG + chr4_2:69569460-69569479 None:intergenic 30.0%
! TTATCTTCTAGTTAAAGGTC+TGG - chr4_2:69569526-69569545 Msa0618640:three_prime_UTR 30.0%
TTGCCAGTAGTAGTATTATA+GGG - chr4_2:69569567-69569586 Msa0618640:three_prime_UTR 30.0%
!!! ATTTTTTAAGGATGAGTAGG+TGG - chr4_2:69569630-69569649 Msa0618640:three_prime_UTR 30.0%
!!! TTTTTTAAGGATGAGTAGGT+GGG - chr4_2:69569631-69569650 Msa0618640:three_prime_UTR 30.0%
ACTTAATCACTTAACCACTT+AGG - chr4_2:69569657-69569676 Msa0618640:three_prime_UTR 30.0%
!! GTTTTGATCATTTGGACAAT+GGG - chr4_2:69569679-69569698 Msa0618640:three_prime_UTR 30.0%
CTAACATCATACATTTAGCA+AGG + chr4_2:69569705-69569724 None:intergenic 30.0%
CATTGTTCTGTAGAAATTCT+TGG - chr4_2:69569850-69569869 Msa0618640:three_prime_UTR 30.0%
! GGTAATTGTTTGAGAGATTT+TGG + chr4_2:69569929-69569948 None:intergenic 30.0%
ACAACATCACAATTGGATTA+GGG + chr4_2:69570043-69570062 None:intergenic 30.0%
TACAACATCACAATTGGATT+AGG + chr4_2:69570044-69570063 None:intergenic 30.0%
GATGTTGTAACTCTCATTAT+AGG - chr4_2:69570055-69570074 Msa0618640:three_prime_UTR 30.0%
TTCAATCGTTTGATAGGAAA+AGG + chr4_2:69570260-69570279 None:intergenic 30.0%
AACTCATTCAATCGTTTGAT+AGG + chr4_2:69570266-69570285 None:intergenic 30.0%
! TCACAGAAAGAAAATTTTGG+AGG + chr4_2:69570383-69570402 None:intergenic 30.0%
AAAAGAGAAATGGAAGATTG+GGG + chr4_2:69570515-69570534 None:intergenic 30.0%
GAAAAGAGAAATGGAAGATT+GGG + chr4_2:69570516-69570535 None:intergenic 30.0%
TGAAAAGAGAAATGGAAGAT+TGG + chr4_2:69570517-69570536 None:intergenic 30.0%
GACCAAGGAGAAAATATAAT+TGG - chr4_2:69570681-69570700 Msa0618640:three_prime_UTR 30.0%
!! TTTTGATGATAAAACGGATC+CGG - chr4_2:69570863-69570882 Msa0618640:three_prime_UTR 30.0%
!!! TCAACTTGTTGCATGTTTTT+TGG - chr4_2:69570891-69570910 Msa0618640:three_prime_UTR 30.0%
! TTTTGTGTGCTAAGATGAAA+GGG - chr4_2:69570952-69570971 Msa0618640:intron 30.0%
TTTATGAACCTTCATTTGCT+CGG + chr4_2:69571108-69571127 None:intergenic 30.0%
ATTCTGTCAAATTCTTGTGT+TGG - chr4_2:69571295-69571314 Msa0618640:intron 30.0%
GAAATTACTGCAAGAAACAA+GGG + chr4_2:69571359-69571378 None:intergenic 30.0%
! TTTCCTCTCTGTTTCTTTAA+AGG - chr4_2:69571375-69571394 Msa0618640:intron 30.0%
! TTCCTCTCTGTTTCTTTAAA+GGG - chr4_2:69571376-69571395 Msa0618640:intron 30.0%
! AACCATTTTATTAGAGAGGA+AGG + chr4_2:69571415-69571434 None:intergenic 30.0%
! GAATATTAACTTGGAGTGTA+AGG - chr4_2:69571537-69571556 Msa0618640:intron 30.0%
ACATATCTGTTATCAACTGT+AGG + chr4_2:69571970-69571989 None:intergenic 30.0%
AAACTACTTGATTGTCTGTT+TGG - chr4_2:69572116-69572135 Msa0618640:intron 30.0%
!!! TCTTGTTGATTTTCTGTTTC+AGG - chr4_2:69572893-69572912 Msa0618640:intron 30.0%
!! TTGTAGCTTTGTAACTTCTT+AGG - chr4_2:69572970-69572989 Msa0618640:intron 30.0%
GACCAAGGAGAAAATATAAT+CGG - chr4_2:69570681-69570700 Msa0618640:three_prime_UTR 30.0%
! ATCCGATTATATTTTCTCCT+TGG + chr4_2:69573117-69573136 None:intergenic 30.0%
! GAATGCTTGACTATTTTATC+TGG + chr4_2:69573148-69573167 None:intergenic 30.0%
! TCTGAACTTTGATGATAAGA+CGG - chr4_2:69573265-69573284 Msa0618640:CDS 30.0%
ATTGTACTGTATGATCTTCA+TGG - chr4_2:69573398-69573417 Msa0618640:CDS 30.0%
TCATTATGATCTTCACCAAA+GGG + chr4_2:69573452-69573471 None:intergenic 30.0%
ATCATTATGATCTTCACCAA+AGG + chr4_2:69573453-69573472 None:intergenic 30.0%
!! GGTTTTATCCAAACATATGA+AGG - chr4_2:69573505-69573524 Msa0618640:CDS 30.0%
TCGTTAGGAATGTAAATGTC+CGG + chr4_2:69567517-69567536 None:intergenic 35.0%
TTCTTAACCCTATCATCGTT+AGG + chr4_2:69567532-69567551 None:intergenic 35.0%
TCTCTCAGCTCTTCTTATAA+CGG + chr4_2:69567647-69567666 None:intergenic 35.0%
!! ACACTAATTGGTGATGCAAT+AGG - chr4_2:69567736-69567755 Msa0618640:three_prime_UTR 35.0%
CATTATGATCTTCACCAAAG+GGG + chr4_2:69567945-69567964 None:intergenic 35.0%
CCATTATGATCTTCACCAAA+GGG + chr4_2:69567946-69567965 None:intergenic 35.0%
ACCATTATGATCTTCACCAA+AGG + chr4_2:69567947-69567966 None:intergenic 35.0%
ATTGCTCACATCTACAAGTT+TGG - chr4_2:69567975-69567994 Msa0618640:three_prime_UTR 35.0%
GTGCATGTTTAGACTTCATT+TGG + chr4_2:69568064-69568083 None:intergenic 35.0%
!! TTTTCATTGGCCATTGGTTA+TGG - chr4_2:69568547-69568566 Msa0618640:three_prime_UTR 35.0%
! TCCATTTTCTCCATAACCAA+TGG + chr4_2:69568560-69568579 None:intergenic 35.0%
TGAAATCTGACGTTGGAATT+AGG - chr4_2:69568814-69568833 Msa0618640:three_prime_UTR 35.0%
ACACACACAAAGTGAAAATG+TGG + chr4_2:69568843-69568862 None:intergenic 35.0%
AGAAAAACACCGCATGAAAA+CGG - chr4_2:69569138-69569157 Msa0618640:three_prime_UTR 35.0%
!! ATTGATTTCCCGTTTTCATG+CGG + chr4_2:69569150-69569169 None:intergenic 35.0%
CACCACTCATTTCTCTTTAT+TGG + chr4_2:69569288-69569307 None:intergenic 35.0%
ACTAAGAGAGAAGGAGAAAA+AGG - chr4_2:69569316-69569335 Msa0618640:three_prime_UTR 35.0%
TAATTGGGGGAAATTGGAAA+CGG + chr4_2:69569375-69569394 None:intergenic 35.0%
TCCAATTTCCCCCAATTATT+TGG - chr4_2:69569377-69569396 Msa0618640:three_prime_UTR 35.0%
ACCAAATAATTGGGGGAAAT+TGG + chr4_2:69569381-69569400 None:intergenic 35.0%
TCACTTCACCAAATAATTGG+GGG + chr4_2:69569388-69569407 None:intergenic 35.0%
GCTTCACTTCACCAAATAAT+TGG + chr4_2:69569391-69569410 None:intergenic 35.0%
GTTGCCAGTAGTAGTATTAT+AGG - chr4_2:69569566-69569585 Msa0618640:three_prime_UTR 35.0%
TGCCAGTAGTAGTATTATAG+GGG - chr4_2:69569568-69569587 Msa0618640:three_prime_UTR 35.0%
ATCCCCTATAATACTACTAC+TGG + chr4_2:69569573-69569592 None:intergenic 35.0%
! ATAGGGGATATTCGAGTTTA+TGG - chr4_2:69569584-69569603 Msa0618640:three_prime_UTR 35.0%
! TAGGGGATATTCGAGTTTAT+GGG - chr4_2:69569585-69569604 Msa0618640:three_prime_UTR 35.0%
CCAAATGATCAAAACCTAAG+TGG + chr4_2:69569674-69569693 None:intergenic 35.0%
!! CCACTTAGGTTTTGATCATT+TGG - chr4_2:69569671-69569690 Msa0618640:three_prime_UTR 35.0%
!! GGTTTTGATCATTTGGACAA+TGG - chr4_2:69569678-69569697 Msa0618640:three_prime_UTR 35.0%
AAATTTGGTTGTTGATCCAG+AGG - chr4_2:69569752-69569771 Msa0618640:three_prime_UTR 35.0%
AATTTGGTTGTTGATCCAGA+GGG - chr4_2:69569753-69569772 Msa0618640:three_prime_UTR 35.0%
! TAAAACATAGTCTTACCCTC+TGG + chr4_2:69569771-69569790 None:intergenic 35.0%
CTTTCTGCACTTAACTACAT+AGG - chr4_2:69569821-69569840 Msa0618640:three_prime_UTR 35.0%
! TTGTTTGAGAGATTTTGGAC+AGG + chr4_2:69569924-69569943 None:intergenic 35.0%
GAGAGTTACAACATCACAAT+TGG + chr4_2:69570050-69570069 None:intergenic 35.0%
CAATCTCTCAAACGACATAA+TGG + chr4_2:69570568-69570587 None:intergenic 35.0%
! CATTATGTCGTTTGAGAGAT+TGG - chr4_2:69570566-69570585 Msa0618640:three_prime_UTR 35.0%
!!! GAATGCTTGACTCTTTTTTC+TGG + chr4_2:69570717-69570736 None:intergenic 35.0%
!! ACACAAATTGGTGATGCAAT+AGG - chr4_2:69570834-69570853 Msa0618640:three_prime_UTR 35.0%
! CTTTTGTGTGCTAAGATGAA+AGG - chr4_2:69570951-69570970 Msa0618640:intron 35.0%
GAAGATCATACAGTACAACT+AGG + chr4_2:69570989-69571008 None:intergenic 35.0%
GTTGTACTGTATGATCTTCA+TGG - chr4_2:69570990-69571009 Msa0618640:intron 35.0%
AGACTCGATTTAAGTGAGAT+TGG - chr4_2:69571014-69571033 Msa0618640:intron 35.0%
CATTATGATCTTCACCAAAG+GGG + chr4_2:69567945-69567964 None:intergenic 35.0%
CCATTATGATCTTCACCAAA+GGG + chr4_2:69567946-69567965 None:intergenic 35.0%
ACCATTATGATCTTCACCAA+AGG + chr4_2:69567947-69567966 None:intergenic 35.0%
ATTGCTCACATCTACAAGTT+TGG - chr4_2:69567975-69567994 Msa0618640:three_prime_UTR 35.0%
TCCAAACGATGTGTATGTTT+GGG + chr4_2:69571208-69571227 None:intergenic 35.0%
TTCCAAACGATGTGTATGTT+TGG + chr4_2:69571209-69571228 None:intergenic 35.0%
TTGCAGGTATAGATGAACTA+AGG - chr4_2:69571266-69571285 Msa0618640:intron 35.0%
!! ACCATAATGGATACATGTCT+CGG + chr4_2:69571329-69571348 None:intergenic 35.0%
CAAGGGAAATCTTACCATAA+TGG + chr4_2:69571342-69571361 None:intergenic 35.0%
GGAAATTACTGCAAGAAACA+AGG + chr4_2:69571360-69571379 None:intergenic 35.0%
! TCTCTGTTTCTTTAAAGGGA+GGG - chr4_2:69571380-69571399 Msa0618640:intron 35.0%
CACCTTCCTCTCTAATAAAA+TGG - chr4_2:69571410-69571429 Msa0618640:intron 35.0%
GGTTTGAACTAGGTTTGATT+TGG - chr4_2:69571431-69571450 Msa0618640:intron 35.0%
!! TGAACTAGGTTTGATTTGGT+TGG - chr4_2:69571435-69571454 Msa0618640:intron 35.0%
! ATGGGTTATGTGAGTAACTT+TGG + chr4_2:69571718-69571737 None:intergenic 35.0%
!! TGATAGCTTGTTTGGTTGAA+AGG + chr4_2:69571839-69571858 None:intergenic 35.0%
!! ACGAAGAATGATAGCTTGTT+TGG + chr4_2:69571847-69571866 None:intergenic 35.0%
AGAAGAGGAAATAAACTTGC+TGG + chr4_2:69572611-69572630 None:intergenic 35.0%
ATGGGGAAAGTTCAAATTTC+AGG + chr4_2:69572925-69572944 None:intergenic 35.0%
!! ACTTTCCCCATTGATTTAAG+TGG - chr4_2:69572934-69572953 Msa0618640:intron 35.0%
GCTTTATGAAAGAAAGACCA+AGG - chr4_2:69573097-69573116 Msa0618640:CDS 35.0%
!!! TCAACTTGTTGCATGTGTTT+TGG - chr4_2:69573299-69573318 Msa0618640:CDS 35.0%
GAAGATCATACAGTACAATC+AGG + chr4_2:69573397-69573416 None:intergenic 35.0%
CATTATGATCTTCACCAAAG+GGG + chr4_2:69567945-69567964 None:intergenic 35.0%
!! TATTGCAGGGGCTTATTTTC+CGG - chr4_2:69567495-69567514 Msa0618640:three_prime_UTR 40.0%
TAAGCCTTGACTCTTCTATC+TGG + chr4_2:69567616-69567635 None:intergenic 40.0%
AGTAAGATTGGTATCCCCTT+TGG - chr4_2:69567928-69567947 Msa0618640:three_prime_UTR 40.0%
CCCTTTGGTGAAGATCATAA+TGG - chr4_2:69567943-69567962 Msa0618640:three_prime_UTR 40.0%
GTCTAAACATGCACATCCTT+TGG - chr4_2:69568070-69568089 Msa0618640:three_prime_UTR 40.0%
! TTTTTGCCATATGGGTAGCT+AGG - chr4_2:69568251-69568270 Msa0618640:three_prime_UTR 40.0%
! GCCATTGGTTATGGAGAAAA+TGG - chr4_2:69568556-69568575 Msa0618640:three_prime_UTR 40.0%
TTCTTCCGTCCCAAAATGTA+AGG + chr4_2:69568738-69568757 None:intergenic 40.0%
ATCCTAATAGTTTGACCGTG+AGG - chr4_2:69568781-69568800 Msa0618640:three_prime_UTR 40.0%
ATCCTCACGGTCAAACTATT+AGG + chr4_2:69568786-69568805 None:intergenic 40.0%
GAAAAACACCGCATGAAAAC+GGG - chr4_2:69569139-69569158 Msa0618640:three_prime_UTR 40.0%
TTCAAGAAGATGGGATTGAC+AGG + chr4_2:69569265-69569284 None:intergenic 40.0%
AGAGAGAAGGAGAAAAAGGT+TGG - chr4_2:69569320-69569339 Msa0618640:three_prime_UTR 40.0%
GAGTTTATGGGCAACAAACA+TGG - chr4_2:69569597-69569616 Msa0618640:three_prime_UTR 40.0%
GTAACTCTCATTATAGGCCA+CGG - chr4_2:69570061-69570080 Msa0618640:three_prime_UTR 40.0%
GGGAGAGAAGAAGATTAAGA+TGG + chr4_2:69570487-69570506 None:intergenic 40.0%
AATGGAAGATTGGGGATTCA+GGG + chr4_2:69570507-69570526 None:intergenic 40.0%
AAATGGAAGATTGGGGATTC+AGG + chr4_2:69570508-69570527 None:intergenic 40.0%
! TGTCGTTTGAGAGATTGGTT+CGG - chr4_2:69570571-69570590 Msa0618640:three_prime_UTR 40.0%
TTAACCTTATCATCCACGCT+GGG + chr4_2:69570630-69570649 None:intergenic 40.0%
TTTAACCTTATCATCCACGC+TGG + chr4_2:69570631-69570650 None:intergenic 40.0%
! CAGTTGCTGTGACAATCTTT+TGG + chr4_2:69570782-69570801 None:intergenic 40.0%
!! ATGCAACAAGTTGATTCAGC+CGG + chr4_2:69570885-69570904 None:intergenic 40.0%
CCCTTTGGTGAAGATCATAA+TGG - chr4_2:69567943-69567962 Msa0618640:three_prime_UTR 40.0%
!! GGTTTTATCCGAGCAAATGA+AGG - chr4_2:69571097-69571116 Msa0618640:intron 40.0%
TCGTTTGGAAAACACCATGT+TGG - chr4_2:69571219-69571238 Msa0618640:intron 40.0%
AACCTCTAATCTCTCCAACA+TGG + chr4_2:69571236-69571255 None:intergenic 40.0%
TCCGAGACATGTATCCATTA+TGG - chr4_2:69571325-69571344 Msa0618640:intron 40.0%
! CTCCCTTTAAAGAAACAGAG+AGG + chr4_2:69571381-69571400 None:intergenic 40.0%
! CTCTCTGTTTCTTTAAAGGG+AGG - chr4_2:69571379-69571398 Msa0618640:intron 40.0%
CATTAGACTCCACGTTTGTT+TGG + chr4_2:69572393-69572412 None:intergenic 40.0%
! GGTGCTGAATAAAGAAGAAG+AGG + chr4_2:69572527-69572546 None:intergenic 40.0%
!! AGGCTTTCTAGCTTGATGTT+TGG - chr4_2:69572869-69572888 Msa0618640:intron 40.0%
! CTTAGGCCATTATGTGCTTT+TGG - chr4_2:69572987-69573006 Msa0618640:intron 40.0%
! CCATTATGTGCTTTTGGATG+CGG - chr4_2:69572993-69573012 Msa0618640:intron 40.0%
! AATGGAGACAAAGGCTAGTT+TGG - chr4_2:69573027-69573046 Msa0618640:CDS 40.0%
!! ATGGAGACAAAGGCTAGTTT+GGG - chr4_2:69573028-69573047 Msa0618640:CDS 40.0%
TTGGGTTACGCTTACATTCT+TGG - chr4_2:69573046-69573065 Msa0618640:CDS 40.0%
TTACGCTTACATTCTTGGCA+TGG - chr4_2:69573051-69573070 Msa0618640:CDS 40.0%
ACATTCTTGGCATGGATGAT+AGG - chr4_2:69573059-69573078 Msa0618640:CDS 40.0%
CATTCTTGGCATGGATGATA+GGG - chr4_2:69573060-69573079 Msa0618640:CDS 40.0%
CTGTGTCAAAGATGCCTAAT+TGG - chr4_2:69573230-69573249 Msa0618640:CDS 40.0%
!! TGCCTAATTGGTGATGCAAT+AGG - chr4_2:69573242-69573261 Msa0618640:CDS 40.0%
GACCTATTGCATCACCAATT+AGG + chr4_2:69567741-69567760 None:intergenic 40.0%
GCACTAAGAGTGAGTAACCA+AGG - chr4_2:69567565-69567584 Msa0618640:three_prime_UTR 45.0%
TGGTCCAGATAGAAGAGTCA+AGG - chr4_2:69567609-69567628 Msa0618640:three_prime_UTR 45.0%
GAGAAATGAGTGGTGACTGT+TGG - chr4_2:69569293-69569312 Msa0618640:three_prime_UTR 45.0%
GACTGTTGGACTAAGAGAGA+AGG - chr4_2:69569307-69569326 Msa0618640:three_prime_UTR 45.0%
TGGTGAAGTGAAGCTTTCTC+AGG - chr4_2:69569397-69569416 Msa0618640:three_prime_UTR 45.0%
ACTAAACAGCACACACCTTG+TGG - chr4_2:69569442-69569461 Msa0618640:three_prime_UTR 45.0%
AGATGATAGACCCTGTCCTT+TGG - chr4_2:69569959-69569978 Msa0618640:three_prime_UTR 45.0%
CAAACATCAGCCAAAGGACA+GGG + chr4_2:69569972-69569991 None:intergenic 45.0%
ATGAGCCAAACATCAGCCAA+AGG + chr4_2:69569978-69569997 None:intergenic 45.0%
TATTCCCAGCGTGGATGATA+AGG - chr4_2:69570623-69570642 Msa0618640:three_prime_UTR 45.0%
AGTGAGATTGGTATCCCCTT+TGG - chr4_2:69571026-69571045 Msa0618640:intron 45.0%
GCTCACATCTACAAGTTTGG+AGG - chr4_2:69567978-69567997 Msa0618640:three_prime_UTR 45.0%
TCTGCTACAAACGCTCTTGT+TGG + chr4_2:69571167-69571186 None:intergenic 45.0%
GCCCAAACATACACATCGTT+TGG - chr4_2:69571204-69571223 Msa0618640:intron 45.0%
CACCATGTTGGAGAGATTAG+AGG - chr4_2:69571231-69571250 Msa0618640:intron 45.0%
! CTGTTTCTTTAAAGGGAGGG+AGG - chr4_2:69571383-69571402 Msa0618640:intron 45.0%
CTAGTCTATAGCGTCTCGTT+TGG + chr4_2:69572292-69572311 None:intergenic 45.0%
! CGTGTTTTGCCAAACAAACG+TGG - chr4_2:69572381-69572400 Msa0618640:intron 45.0%
! CCGCATCCAAAAGCACATAA+TGG + chr4_2:69572996-69573015 None:intergenic 45.0%
! TTATGTGCTTTTGGATGCGG+AGG - chr4_2:69572996-69573015 Msa0618640:intron 45.0%
CTTCATGGTAGACTCGATCT+AGG - chr4_2:69573413-69573432 Msa0618640:CDS 45.0%
AGACTCGATCTAGGAGAGAT+TGG - chr4_2:69573422-69573441 Msa0618640:CDS 45.0%
GCTCACATCTACAAGCTTAG+AGG - chr4_2:69573484-69573503 Msa0618640:CDS 45.0%
!! ATATTATTAATAAAAAATCA+AGG - chr4_2:69570436-69570455 Msa0618640:three_prime_UTR 5.0%
TTGCCATATGGGTAGCTAGG+TGG - chr4_2:69568254-69568273 Msa0618640:three_prime_UTR 50.0%
TAGCCACCTAGCTACCCATA+TGG + chr4_2:69568260-69568279 None:intergenic 50.0%
CCAAACATCAGCCAAAGGAC+AGG + chr4_2:69569973-69569992 None:intergenic 50.0%
CCTGTCCTTTGGCTGATGTT+TGG - chr4_2:69569970-69569989 Msa0618640:three_prime_UTR 50.0%
!! GAGAGATTGGTTCGGCAATC+CGG - chr4_2:69570579-69570598 Msa0618640:three_prime_UTR 50.0%
!! AGAGATTGGTTCGGCAATCC+GGG - chr4_2:69570580-69570599 Msa0618640:three_prime_UTR 50.0%
GGGCATTTATATTCCCAGCG+TGG - chr4_2:69570614-69570633 Msa0618640:three_prime_UTR 50.0%
GCACTAAGAGTGAGAGACCA+AGG - chr4_2:69570666-69570685 Msa0618640:three_prime_UTR 50.0%
!! GAGGTTGAGAAGTGCTTTGC+AGG - chr4_2:69571250-69571269 Msa0618640:intron 50.0%
GAGGAGGAGAATGGAGACAA+AGG - chr4_2:69573018-69573037 Msa0618640:intron 50.0%
GGAGAGATTGGTATCCCCTT+TGG - chr4_2:69573434-69573453 Msa0618640:CDS 50.0%
GGAAGCCTGCGTTTCGTCTA+TGG - chr4_2:69570082-69570101 Msa0618640:three_prime_UTR 55.0%
AGCCTGCGTTTCGTCTATGG+TGG - chr4_2:69570085-69570104 Msa0618640:three_prime_UTR 55.0%
CACCACCATAGACGAAACGC+AGG + chr4_2:69570090-69570109 None:intergenic 55.0%
TCGGCAATCCGGGAACAGTT+TGG - chr4_2:69570590-69570609 Msa0618640:three_prime_UTR 55.0%
CGGCAATCCGGGAACAGTTT+GGG - chr4_2:69570591-69570610 Msa0618640:three_prime_UTR 55.0%
GGCAATCCGGGAACAGTTTG+GGG - chr4_2:69570592-69570611 Msa0618640:three_prime_UTR 55.0%
GCAATCCGGGAACAGTTTGG+GGG - chr4_2:69570593-69570612 Msa0618640:three_prime_UTR 55.0%
CAATCCGGGAACAGTTTGGG+GGG - chr4_2:69570594-69570613 Msa0618640:three_prime_UTR 55.0%
AATGCCCCCCAAACTGTTCC+CGG + chr4_2:69570601-69570620 None:intergenic 55.0%
! TGTGCTTTTGGATGCGGAGG+AGG - chr4_2:69572999-69573018 Msa0618640:intron 55.0%
AGACGAAACGCAGGCTTCCG+TGG + chr4_2:69570081-69570100 None:intergenic 60.0%
! GCTTTTGGATGCGGAGGAGG+AGG - chr4_2:69573002-69573021 Msa0618640:intron 60.0%
GATGCGGAGGAGGAGGAGAA+TGG - chr4_2:69573009-69573028 Msa0618640:intron 60.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 69567487 69573552 69567487 ID=Msa0618640;Name=Msa0618640
chr4_2 mRNA 69567487 69573552 69567487 ID=Msa0618640-mRNA-1;Parent=Msa0618640;Name=Msa0618640-mRNA-1;_AED=0.20;_eAED=0.20;_QI=0|0|0.5|1|0|0|2|3425|194
chr4_2 exon 69567487 69570962 69567487 ID=Msa0618640-mRNA-1:exon:20126;Parent=Msa0618640-mRNA-1
chr4_2 exon 69573019 69573552 69573019 ID=Msa0618640-mRNA-1:exon:20125;Parent=Msa0618640-mRNA-1
chr4_2 CDS 69573019 69573552 69573019 ID=Msa0618640-mRNA-1:cds;Parent=Msa0618640-mRNA-1
chr4_2 CDS 69570912 69570962 69570912 ID=Msa0618640-mRNA-1:cds;Parent=Msa0618640-mRNA-1
chr4_2 three_prime_UTR 69567487 69570911 69567487 ID=Msa0618640-mRNA-1:three_prime_utr;Parent=Msa0618640-mRNA-1
Gene Sequence

>Msa0618640

ATGGAAGATATTGCAGGGGCTTATTTTCCGGACATTTACATTCCTAACGATGATAGGGTTAAGAAACAAATTATGAGTGCACTAAGAGTGAGTAACCAAGGAGAAAATTTAATCGGATTATGTGGTCCAGATAGAAGAGTCAAGGCTTATGTGAAAACCGTTATAAGAAGAGCTGAGAGAGATCAATTGTTCCAAAAGATTGTCACAACAACAACTGTGACAAAGATGTCAAACATTACAAAGATTCAAACACTAATTGGTGATGCAATAGGTCTGAATTTTGATGATACGACAGATATAGCTGAATCTACTTGTTGCATGTGTTTTGTTAATAATAAAAGATTGACAACTGCAGAAAGAGCTCTTCTTCTGTGTGCTAAGATGAAAGAGCTTGAAACAGTCCTAGTTGTAGTGTATGATCTTTATGGTAGACTTGATTTAAGTAAGATTGGTATCCCCTTTGGTGAAGATCATAATGGTTGCAAGATATTGCTCACATCTACAAGTTTGGAGATTTTATCCAAACAAATGAAGGCCACGGAAGCCTGCGTTTCGTCTATGGTGGTGCCAAAAAAACAAGTTTGA
Protein sequence

>Msa0618640

MEDIAGAYFPDIYIPNDDRVKKQIMSALRVSNQGENLIGLCGPDRRVKAYVKTVIRRAERDQLFQKIVTTTTVTKMSNITKIQTLIGDAIGLNFDDTTDIAESTCCMCFVNNKRLTTAERALLLCAKMKELETVLVVVYDLYGRLDLSKIGIPFGEDHNGCKILLTSTSLEILSKQMKATEACVSSMVVPKKQV*