AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0621610

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0621610 MtrunA17_Chr2g0286951 100.000 346 0 0 1 346 1 346 0.0 723
Msa0621610 MtrunA17_Chr4g0066371 72.254 346 95 1 1 346 1 345 0.0 518
Msa0621610 MtrunA17_Chr6g0486661 62.829 304 110 2 43 343 80 383 1.81e-128 393
Msa0621610 MtrunA17_Chr2g0295401 29.394 330 177 9 45 346 58 359 2.24e-32 124
Msa0621610 MtrunA17_Chr7g0272061 28.743 334 178 9 45 344 57 364 1.86e-29 116
Msa0621610 MtrunA17_Chr1g0192421 30.435 322 171 12 44 339 54 348 9.06e-28 111
Msa0621610 MtrunA17_Chr7g0243571 26.603 312 172 5 48 325 58 346 8.71e-27 110
Msa0621610 MtrunA17_Chr3g0105371 29.801 302 151 13 52 342 88 339 1.95e-26 107
Msa0621610 MtrunA17_Chr4g0004971 28.622 283 157 5 69 324 60 324 3.07e-26 107
Msa0621610 MtrunA17_Chr1g0150451 29.932 294 159 11 55 341 70 323 3.87e-26 106
Msa0621610 MtrunA17_Chr3g0136711 29.667 300 161 11 45 341 28 280 6.87e-26 105
Msa0621610 MtrunA17_Chr4g0067711 29.866 298 157 9 53 324 62 333 1.16e-25 105
Msa0621610 MtrunA17_Chr3g0105351 28.571 294 154 11 60 342 67 315 2.32e-25 104
Msa0621610 MtrunA17_Chr7g0249421 28.882 322 156 8 53 324 67 365 6.43e-25 105
Msa0621610 MtrunA17_Chr7g0221691 28.956 297 149 6 69 323 111 387 1.10e-24 104
Msa0621610 MtrunA17_Chr5g0430481 29.470 302 152 13 52 342 124 375 1.84e-24 102
Msa0621610 MtrunA17_Chr1g0151781 30.252 238 126 4 87 302 68 287 2.33e-24 102
Msa0621610 MtrunA17_Chr4g0022171 27.476 313 170 9 69 343 79 372 3.53e-23 100
Msa0621610 MtrunA17_Chr8g0342431 29.470 302 143 9 69 320 68 349 4.73e-23 99.0
Msa0621610 MtrunA17_Chr2g0324061 27.885 312 169 9 69 343 73 365 5.08e-23 99.8
Msa0621610 MtrunA17_Chr1g0212961 29.961 257 137 10 85 341 124 337 4.12e-22 95.5
Msa0621610 MtrunA17_Chr4g0065141 28.044 271 137 10 84 339 121 348 2.87e-21 93.6
Msa0621610 MtrunA17_Chr3g0126511 27.839 273 156 9 70 341 108 340 1.43e-20 91.7
Msa0621610 MtrunA17_Chr5g0426141 27.444 266 151 11 78 342 58 282 5.71e-18 82.8
Msa0621610 MtrunA17_Chr2g0293811 27.106 273 136 8 87 339 100 329 9.41e-18 83.6
Msa0621610 MtrunA17_Chr3g0115851 26.217 267 153 9 78 342 58 282 2.59e-17 80.9
Msa0621610 MtrunA17_Chr5g0407161 26.038 265 139 7 87 339 124 343 2.70e-17 82.0
Msa0621610 MtrunA17_Chr4g0051661 29.412 255 136 13 87 339 183 395 3.43e-16 79.0
Msa0621610 MtrunA17_Chr3g0112291 29.200 250 125 12 67 299 103 317 7.85e-16 77.8
Msa0621610 MtrunA17_Chr1g0194191 25.714 280 145 9 87 346 118 354 1.52e-15 77.0
Msa0621610 MtrunA17_Chr1g0171571 27.597 308 176 11 45 342 375 645 3.18e-15 76.6
Msa0621610 MtrunA17_Chr8g0389921 26.222 225 127 5 85 303 196 387 3.58e-15 76.3
Msa0621610 MtrunA17_Chr5g0396731 29.870 231 113 7 87 305 107 300 3.39e-14 72.8
Msa0621610 MtrunA17_Chr3g0144111 28.405 257 122 10 87 339 27 225 4.01e-14 71.6
Msa0621610 MtrunA17_Chr7g0256751 27.969 261 125 11 87 314 69 299 8.26e-14 72.0
Msa0621610 MtrunA17_Chr7g0256771 27.888 251 126 10 87 307 70 295 2.19e-13 70.1
Msa0621610 MtrunA17_Chr3g0133801 27.609 297 165 14 60 341 34 295 7.70e-13 68.9
Msa0621610 MtrunA17_Chr4g0001761 25.159 314 151 11 84 344 49 331 1.05e-12 68.6
Msa0621610 MtrunA17_Chr5g0435721 27.586 232 110 9 86 298 222 414 9.60e-12 65.9
Msa0621610 MtrunA17_Chr7g0271971 28.328 293 164 13 67 346 43 302 3.14e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0621610 AT3G06270.1 71.920 349 93 2 1 346 1 347 3.02e-179 500
Msa0621610 AT2G20050.2 60.241 332 127 1 17 343 68 399 6.04e-131 400
Msa0621610 AT2G20050.1 60.241 332 127 1 17 343 68 399 9.08e-131 400
Msa0621610 AT5G26010.1 33.735 249 134 6 69 305 56 285 4.23e-30 117
Msa0621610 AT5G27930.1 27.219 338 193 10 29 339 41 352 1.12e-29 117
Msa0621610 AT5G27930.2 27.219 338 193 10 29 339 41 352 1.12e-29 117
Msa0621610 AT3G05640.2 28.994 338 186 10 14 324 20 330 1.36e-29 117
Msa0621610 AT3G05640.1 28.994 338 186 10 14 324 20 330 1.36e-29 117
Msa0621610 AT3G05640.3 28.994 338 186 10 14 324 20 330 1.36e-29 117
Msa0621610 AT4G32950.1 31.298 262 152 8 89 341 74 316 9.68e-28 111
Msa0621610 AT4G31750.2 29.932 294 159 11 55 341 26 279 1.33e-26 107
Msa0621610 AT1G07160.1 29.934 304 149 12 52 341 123 376 1.37e-26 108
Msa0621610 AT5G10740.1 30.272 294 158 11 55 341 26 279 1.39e-26 108
Msa0621610 AT5G10740.2 30.272 294 158 11 55 341 26 279 1.39e-26 108
Msa0621610 AT4G03415.1 30.573 314 160 10 69 343 80 374 1.43e-26 110
Msa0621610 AT4G03415.3 30.573 314 160 10 69 343 80 374 1.43e-26 110
Msa0621610 AT4G03415.2 30.573 314 160 10 69 343 80 374 1.43e-26 110
Msa0621610 AT4G31750.1 29.932 294 159 11 55 341 26 279 1.59e-26 107
Msa0621610 AT4G31750.3 29.932 294 159 11 55 341 26 279 1.59e-26 107
Msa0621610 AT5G01700.1 27.027 296 175 7 69 341 11 288 4.45e-26 106
Msa0621610 AT5G01700.2 27.027 296 175 7 69 341 60 337 1.01e-25 106
Msa0621610 AT5G27930.3 29.431 299 158 10 29 300 41 313 5.24e-25 103
Msa0621610 AT5G24940.1 30.620 258 138 9 85 341 62 279 9.28e-25 104
Msa0621610 AT5G36250.1 27.303 304 155 8 69 324 80 365 2.40e-23 100
Msa0621610 AT4G32950.2 34.928 209 110 7 89 290 74 263 4.20e-23 97.4
Msa0621610 AT1G03590.1 27.974 311 169 9 69 343 73 364 9.43e-23 99.0
Msa0621610 AT1G16220.1 26.398 322 174 7 69 345 77 380 1.74e-22 98.6
Msa0621610 AT5G53140.1 26.642 274 160 9 70 342 115 348 5.00e-22 96.7
Msa0621610 AT5G53140.2 26.642 274 160 9 70 342 115 348 5.00e-22 96.7
Msa0621610 AT1G43900.1 31.022 274 147 10 70 342 137 369 8.92e-22 95.5
Msa0621610 AT2G30020.1 30.100 299 156 13 52 342 140 393 1.21e-21 95.5
Msa0621610 AT3G16800.2 27.055 292 172 6 69 339 75 346 1.36e-21 94.7
Msa0621610 AT3G16800.1 27.055 292 172 6 69 339 75 346 1.36e-21 94.7
Msa0621610 AT3G16800.3 27.055 292 172 6 69 339 75 346 1.36e-21 94.7
Msa0621610 AT1G79630.1 30.233 215 128 5 135 345 207 403 7.79e-21 94.0
Msa0621610 AT1G79630.2 30.233 215 128 5 135 345 137 333 7.93e-21 93.2
Msa0621610 AT1G79630.7 30.233 215 128 5 135 345 137 333 7.93e-21 93.2
Msa0621610 AT1G79630.8 30.233 215 128 5 135 345 203 399 8.92e-21 93.6
Msa0621610 AT1G79630.6 30.233 215 128 5 135 345 97 293 9.83e-21 92.8
Msa0621610 AT1G79630.3 30.233 215 128 5 135 345 97 293 9.83e-21 92.8
Msa0621610 AT1G79630.4 30.233 215 128 5 135 345 97 293 9.83e-21 92.8
Msa0621610 AT1G79630.5 30.233 215 128 5 135 345 97 293 9.83e-21 92.8
Msa0621610 AT5G36250.2 26.415 318 154 9 69 324 80 379 3.94e-20 91.7
Msa0621610 AT5G36250.3 28.519 270 127 8 69 290 80 331 5.47e-20 90.1
Msa0621610 AT3G16800.4 26.937 271 157 6 90 339 1 251 7.33e-20 88.2
Msa0621610 AT3G16800.6 26.937 271 157 6 90 339 1 251 7.33e-20 88.2
Msa0621610 AT3G02750.2 32.812 192 109 4 111 300 177 350 1.56e-19 89.7
Msa0621610 AT3G02750.1 32.812 192 109 4 111 300 177 350 1.56e-19 89.7
Msa0621610 AT1G78200.5 27.239 268 154 10 77 342 10 238 7.07e-19 85.1
Msa0621610 AT1G78200.4 27.239 268 154 10 77 342 10 238 7.07e-19 85.1
Msa0621610 AT1G78200.1 27.239 268 154 10 77 342 55 283 9.84e-19 85.5
Msa0621610 AT1G78200.3 27.239 268 154 10 77 342 55 283 9.84e-19 85.5
Msa0621610 AT1G78200.2 27.239 268 154 10 77 342 55 283 9.84e-19 85.5
Msa0621610 AT2G40180.1 27.509 269 139 12 87 342 161 386 1.23e-17 83.6
Msa0621610 AT3G51470.2 27.007 274 145 9 77 339 52 281 1.73e-17 82.4
Msa0621610 AT3G51470.1 27.007 274 145 9 77 339 96 325 2.39e-17 82.4
Msa0621610 AT2G20630.1 26.848 257 147 10 87 342 63 279 5.81e-16 77.4
Msa0621610 AT2G33700.1 29.183 257 141 9 87 339 123 342 6.19e-16 78.6
Msa0621610 AT5G51760.2 31.301 246 107 10 85 303 112 322 2.80e-15 76.6
Msa0621610 AT5G51760.1 31.301 246 107 10 85 303 142 352 3.24e-15 76.6
Msa0621610 AT2G20630.2 26.877 253 144 10 87 338 63 275 3.63e-15 75.5
Msa0621610 AT1G09160.2 28.523 298 159 15 67 347 43 303 1.71e-14 74.7
Msa0621610 AT1G09160.1 28.523 298 159 15 67 347 43 303 1.71e-14 74.7
Msa0621610 AT4G28400.3 27.444 266 149 13 78 341 37 260 5.34e-14 71.6
Msa0621610 AT4G28400.1 27.444 266 149 13 78 341 59 282 7.31e-14 71.6
Msa0621610 AT1G22280.1 24.903 257 152 11 87 342 65 281 7.57e-14 71.2
Msa0621610 AT2G34740.1 25.000 272 161 11 70 339 102 332 9.03e-14 72.0
Msa0621610 AT1G34750.2 25.940 266 155 11 78 342 58 282 2.46e-13 70.1
Msa0621610 AT1G34750.1 25.940 266 155 11 78 342 58 282 2.46e-13 70.1
Msa0621610 AT1G34750.3 25.940 266 155 11 78 342 58 282 2.46e-13 70.1
Msa0621610 AT1G68410.4 29.392 296 157 14 67 346 48 307 3.37e-13 70.5
Msa0621610 AT1G68410.3 29.392 296 157 14 67 346 48 307 3.37e-13 70.5
Msa0621610 AT1G68410.1 29.392 296 157 14 67 346 48 307 3.37e-13 70.5
Msa0621610 AT1G68410.2 29.392 296 157 14 67 346 48 307 3.37e-13 70.5
Msa0621610 AT5G59220.1 28.319 226 114 11 86 298 147 337 7.52e-13 69.7
Msa0621610 AT1G22280.3 24.715 263 151 12 87 342 65 287 5.60e-12 65.9
Msa0621610 AT1G47380.1 26.117 291 163 13 67 341 39 293 7.07e-11 63.5

Find 119 sgRNAs with CRISPR-Local

Find 217 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATTCAATACACTTTCTTTA+TGG 0.100708 4_2:+73786947 Msa0621610:three_prime_UTR
GCGATTACGGTGTTGGTTAT+AGG 0.202635 4_2:+73785058 Msa0621610:CDS
CGATTACGGTGTTGGTTATA+GGG 0.213735 4_2:+73785059 Msa0621610:CDS
AATCCTAGTGTTCATTTCTT+TGG 0.226643 4_2:+73784848 Msa0621610:CDS
GAAGAATTGTACGTTTGTTT+TGG 0.230319 4_2:-73784180 None:intergenic
TATGATGGACATGGTGAATT+TGG 0.264187 4_2:+73784875 Msa0621610:CDS
TATGAACTTAAATATTCTTT+TGG 0.269712 4_2:+73786890 Msa0621610:exon
TCAAATATGGGTTGTGTTCA+TGG 0.271492 4_2:+73784599 Msa0621610:exon
CTTTAGCATGTTATTTATAT+AGG 0.277979 4_2:+73786914 Msa0621610:three_prime_UTR
TGTGTGAGTATGTGCTTCTT+AGG 0.299664 4_2:-73784686 None:intergenic
TCATATTCATCTCTCCTAAA+TGG 0.301253 4_2:-73785181 None:intergenic
CCTCTGATCAGACACCATTT+AGG 0.314388 4_2:+73785167 Msa0621610:CDS
GTGCATTTCCTCTGCATCCT+AGG 0.321225 4_2:+73784285 Msa0621610:five_prime_UTR
TAATGATTCTGCATTGTTAG+AGG 0.332752 4_2:+73784946 Msa0621610:CDS
ACCAAGATTGTTATTGTGTT+AGG 0.333236 4_2:+73784810 Msa0621610:CDS
CAAACCCTAGAAGCAAAATT+AGG 0.335702 4_2:-73784454 None:intergenic
TCACCCCAAGTCTGAATATC+AGG 0.338735 4_2:-73785265 None:intergenic
GATGCATGTGCTGCCATTGC+TGG 0.362529 4_2:+73786781 Msa0621610:CDS
CTGGAGAGTCTTACAAACTA+TGG 0.364755 4_2:+73786800 Msa0621610:CDS
TCCTAACACAATAACAATCT+TGG 0.372739 4_2:-73784811 None:intergenic
GCTGAATTATAAGCCTTAAC+AGG 0.373479 4_2:-73784971 None:intergenic
AAGGAGTGTAGGGGATAGTT+TGG 0.383776 4_2:+73785360 Msa0621610:CDS
AAGCCAAACTGTTGTTGATA+TGG 0.388203 4_2:+73785489 Msa0621610:CDS
TGCTTCACACAATTTCACTA+TGG 0.393839 4_2:+73784733 Msa0621610:CDS
TCCCTAGAGCCACCAATTGA+TGG 0.398728 4_2:-73784647 None:intergenic
GAGGGCGGTGTTGGCTGTTA+AGG 0.406475 4_2:+73785114 Msa0621610:CDS
TTGGGGTGATGAAGAGAGTC+AGG 0.411344 4_2:+73785279 Msa0621610:CDS
CAACAACAGTTTGGCTTGAC+AGG 0.412932 4_2:-73785483 None:intergenic
GAGTGGTACTACCGCGATTA+CGG 0.413802 4_2:+73785045 Msa0621610:CDS
CATTTCTTTGGTGTTTATGA+TGG 0.417928 4_2:+73784860 Msa0621610:CDS
GATAGTTTGGCTGAGACTAT+TGG 0.418790 4_2:+73785373 Msa0621610:CDS
GTTCAGAATGGGATGATTCC+TGG 0.421871 4_2:+73785325 Msa0621610:CDS
GTGTGAGTATGTGCTTCTTA+GGG 0.422718 4_2:-73784685 None:intergenic
TGCGGTTCCAGAGGTGTTAA+CGG 0.424209 4_2:+73785402 Msa0621610:CDS
CTATCCTGATTCACCCGATA+AGG 0.432231 4_2:+73784784 Msa0621610:CDS
CCATTCTGAACCCACAATCT+AGG 0.435294 4_2:-73785313 None:intergenic
TGCATTTCCTCTGCATCCTA+GGG 0.436158 4_2:+73784286 Msa0621610:five_prime_UTR
ATACCATATCAACAACAGTT+TGG 0.440907 4_2:-73785492 None:intergenic
ATCCCTCTGAATTTCTCTCT+TGG 0.444279 4_2:+73784115 Msa0621610:five_prime_UTR
CTAGAAGCAAAATTAGGAAA+AGG 0.445550 4_2:-73784448 None:intergenic
CGTTATCCTGCACCATCAAT+TGG 0.450895 4_2:+73784635 Msa0621610:CDS
AAAGAGAATGATTTGAAGAA+AGG 0.452178 4_2:-73784345 None:intergenic
TGTTCTTTGAAGTACAATGC+TGG 0.457699 4_2:+73784481 Msa0621610:five_prime_UTR
AGCTGAACCGTTAACACCTC+TGG 0.458512 4_2:-73785409 None:intergenic
TTCTTTGTCGTTGCAAGTGA+TGG 0.461813 4_2:+73785448 Msa0621610:CDS
TGATCAGGTGGAAGGGCATA+AGG 0.467631 4_2:+73785240 Msa0621610:CDS
AGTATGTGCTTCTTAGGGAC+AGG 0.475916 4_2:-73784680 None:intergenic
CTAGATTGTGGGTTCAGAAT+GGG 0.476123 4_2:+73785314 Msa0621610:CDS
TAGAAGCAAAATTAGGAAAA+GGG 0.478018 4_2:-73784447 None:intergenic
GAGAAGAGAACGTGTGAAGT+GGG 0.480992 4_2:-73784221 None:intergenic
AATGGCAGCACATGCATCAC+GGG 0.483050 4_2:-73786776 None:intergenic
GTGATGATCCTCCTAGATTG+TGG 0.486182 4_2:+73785302 Msa0621610:CDS
TAGGGTGTTGAGTGTTGATC+AGG 0.490225 4_2:+73785225 Msa0621610:CDS
CCTAGATTGTGGGTTCAGAA+TGG 0.493333 4_2:+73785313 Msa0621610:CDS
ATTATCATTGTTCAGATCAA+AGG 0.494077 4_2:+73786856 Msa0621610:CDS
ATTGTTAGAGGATCCTGTTA+AGG 0.497099 4_2:+73784958 Msa0621610:CDS
TTTGGGAGAGAGAGAATCGA+AGG 0.500845 4_2:-73784162 None:intergenic
CACCATCAATTGGTGGCTCT+AGG 0.503277 4_2:+73784645 Msa0621610:CDS
CGGTGTTGGCTGTTAAGGAC+GGG 0.504994 4_2:+73785119 Msa0621610:CDS
GGATCCTGATATTCAGACTT+GGG 0.506805 4_2:+73785261 Msa0621610:CDS
CGGGAATAGAATTGTAGCTC+AGG 0.508303 4_2:+73785138 Msa0621610:CDS
GTTCTTTGAAGTACAATGCT+GGG 0.508637 4_2:+73784482 Msa0621610:five_prime_UTR
TACTACCGCGATTACGGTGT+TGG 0.510125 4_2:+73785051 Msa0621610:CDS
ACCATCAATTGGTGGCTCTA+GGG 0.510461 4_2:+73784646 Msa0621610:CDS
TGATGATCCTCCTAGATTGT+GGG 0.511280 4_2:+73785303 Msa0621610:CDS
TGGTGATTCGAGGGCGGTGT+TGG 0.514167 4_2:+73785105 Msa0621610:CDS
TTGCGAATGTTGGTGATTCG+AGG 0.518226 4_2:+73785095 Msa0621610:CDS
GAGCCACCAATTGATGGTGC+AGG 0.519755 4_2:-73784641 None:intergenic
AGTGAAGCTTAGTGGTGCTA+GGG 0.524175 4_2:+73785207 Msa0621610:CDS
ACGCTTTATGTTGCGAATGT+TGG 0.525449 4_2:+73785085 Msa0621610:CDS
TGGAGAACTGAAAACTCCAT+TGG 0.529686 4_2:+73786967 Msa0621610:three_prime_UTR
TATGAGAGAGTGAAGCTTAG+TGG 0.530192 4_2:+73785199 Msa0621610:CDS
ACGACAAAGAAAAGATGATC+CGG 0.531459 4_2:-73785436 None:intergenic
CTCCTTGTAAAAGCAGCTCC+AGG 0.532586 4_2:-73785343 None:intergenic
GAGTGAAGCTTAGTGGTGCT+AGG 0.533858 4_2:+73785206 Msa0621610:CDS
ATTGTTACTTAAATCACCAA+TGG 0.535196 4_2:-73786983 None:intergenic
GCGGTGTTGGCTGTTAAGGA+CGG 0.537963 4_2:+73785118 Msa0621610:CDS
GATGGACATGGTGAATTTGG+TGG 0.538098 4_2:+73784878 Msa0621610:CDS
CAATGGCAGCACATGCATCA+CGG 0.539402 4_2:-73786777 None:intergenic
TTTAAGTTCATACATGAGAC+GGG 0.542090 4_2:-73786879 None:intergenic
CAAACTATGGTTGGAACATG+AGG 0.543259 4_2:+73786813 Msa0621610:CDS
ATTTAAGTTCATACATGAGA+CGG 0.543877 4_2:-73786880 None:intergenic
GCAGAGGAAATGCACAAAGA+AGG 0.544312 4_2:-73784277 None:intergenic
CTATATAAATAACATGCTAA+AGG 0.546356 4_2:-73786913 None:intergenic
AGAGAAGAGAACGTGTGAAG+TGG 0.547448 4_2:-73784222 None:intergenic
GAAATGCACAAAGAAGGAAA+AGG 0.551228 4_2:-73784271 None:intergenic
AGGATCCTGATATTCAGACT+TGG 0.558046 4_2:+73785260 Msa0621610:CDS
TGAAGTGGGGCTTTGTGAAA+AGG 0.558853 4_2:-73784207 None:intergenic
AGAGTCTTACAAACTATGGT+TGG 0.559644 4_2:+73786804 Msa0621610:CDS
TGGGGCTTTGTGAAAAGGAA+AGG 0.563336 4_2:-73784202 None:intergenic
GTCCAAGAGAGAAATTCAGA+GGG 0.572587 4_2:-73784117 None:intergenic
TATCCTGCACCATCAATTGG+TGG 0.575659 4_2:+73784638 Msa0621610:CDS
AGTCCAAGAGAGAAATTCAG+AGG 0.578189 4_2:-73784118 None:intergenic
TGGTGTTATTGCGGTTCCAG+AGG 0.582043 4_2:+73785393 Msa0621610:CDS
GTTAACGGTTCAGCTTAAGC+CGG 0.593244 4_2:+73785417 Msa0621610:CDS
TTGAGTGTTGATCAGGTGGA+AGG 0.593641 4_2:+73785232 Msa0621610:CDS
TTTGGTGTTTATGATGGACA+TGG 0.600671 4_2:+73784866 Msa0621610:CDS
AAGAGAGAAATTCAGAGGGA+TGG 0.601529 4_2:-73784113 None:intergenic
AGCAGAACCCTAGGATGCAG+AGG 0.603200 4_2:-73784293 None:intergenic
GAGATTGATGATTGTATGAG+TGG 0.605602 4_2:+73785028 Msa0621610:CDS
TGTGTTAGGACGCAATTGCA+AGG 0.606214 4_2:+73784824 Msa0621610:CDS
CCTAAATGGTGTCTGATCAG+AGG 0.607645 4_2:-73785167 None:intergenic
ACACCAAAGAAATGAACACT+AGG 0.610056 4_2:-73784851 None:intergenic
TGCGAATGTTGGTGATTCGA+GGG 0.614063 4_2:+73785096 Msa0621610:CDS
TGAGACTATTGGTGTTATTG+CGG 0.618341 4_2:+73785384 Msa0621610:CDS
GATTACGGTGTTGGTTATAG+GGG 0.627105 4_2:+73785060 Msa0621610:CDS
TTTGTAAGACTCTCCAGCAA+TGG 0.632607 4_2:-73786794 None:intergenic
TATAACCAACACCGTAATCG+CGG 0.633218 4_2:-73785056 None:intergenic
GGTGTTGAGTGTTGATCAGG+TGG 0.646452 4_2:+73785228 Msa0621610:CDS
TTCTGAACCCACAATCTAGG+AGG 0.648400 4_2:-73785310 None:intergenic
AGAAGAGAACGTGTGAAGTG+GGG 0.650950 4_2:-73784220 None:intergenic
AAACTATGGTTGGAACATGA+GGG 0.651163 4_2:+73786814 Msa0621610:CDS
TGAGTGTTGATCAGGTGGAA+GGG 0.658387 4_2:+73785233 Msa0621610:CDS
GATCCTGATATTCAGACTTG+GGG 0.666905 4_2:+73785262 Msa0621610:CDS
TGGGGTGATGAAGAGAGTCA+GGG 0.677138 4_2:+73785280 Msa0621610:CDS
TACTCTGTACTTACTCAGAG+AGG 0.692582 4_2:+73784758 Msa0621610:CDS
ATGGCAGCACATGCATCACG+GGG 0.706045 4_2:-73786775 None:intergenic
AGAAGCACATACTCACACAA+AGG 0.715753 4_2:+73784690 Msa0621610:CDS
GAATGTTGGTGATTCGAGGG+CGG 0.720824 4_2:+73785099 Msa0621610:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAAAAAAAAATGCAATTTT+AGG - chr4_2:73784538-73784557 None:intergenic 10.0%
!! AAAAAAAAAAAATCACTTAT+AGG - chr4_2:73786035-73786054 None:intergenic 10.0%
!! ATTAGTATTAACTATTAATT+AGG + chr4_2:73786288-73786307 Msa0621610:intron 10.0%
!! ATCATAACATATATATATAT+AGG - chr4_2:73786692-73786711 None:intergenic 10.0%
!!! TAATTTTGTTAAGAATAAGT+TGG + chr4_2:73784910-73784929 Msa0621610:CDS 15.0%
!! AATTAGTAAATACCTTAAAA+AGG - chr4_2:73785572-73785591 None:intergenic 15.0%
!!! ATTTAAAATGTTGTCAAATA+AGG + chr4_2:73785754-73785773 Msa0621610:intron 15.0%
!!! TTTAAAATGTTGTCAAATAA+GGG + chr4_2:73785755-73785774 Msa0621610:intron 15.0%
!!! AGTGATTTTTTTTTTTTGTA+TGG + chr4_2:73786038-73786057 Msa0621610:intron 15.0%
!!! TTTTAGGAAAGATAATATAT+GGG - chr4_2:73786095-73786114 None:intergenic 15.0%
!!! ATTTTAGGAAAGATAATATA+TGG - chr4_2:73786096-73786115 None:intergenic 15.0%
!! ATATATTATCTTTCCTAAAA+TGG + chr4_2:73786095-73786114 Msa0621610:intron 15.0%
!! TAAATAAAACATTACCTAAA+GGG - chr4_2:73786217-73786236 None:intergenic 15.0%
!! ATAAATAAAACATTACCTAA+AGG - chr4_2:73786218-73786237 None:intergenic 15.0%
!!! GTTTTATTTATGTTAAACTA+TGG + chr4_2:73786227-73786246 Msa0621610:intron 15.0%
!!! TATGAACTTAAATATTCTTT+TGG + chr4_2:73786890-73786909 Msa0621610:exon 15.0%
!!! TGTAGTTTGATTTTCAAATA+TGG + chr4_2:73784586-73784605 Msa0621610:exon 20.0%
!!! GTAGTTTGATTTTCAAATAT+GGG + chr4_2:73784587-73784606 Msa0621610:exon 20.0%
!!! TTCTTTCTATTTCCTTTTTA+AGG + chr4_2:73785557-73785576 Msa0621610:intron 20.0%
!!! TTACTACATTTTCTTTTTTG+TGG + chr4_2:73785859-73785878 Msa0621610:intron 20.0%
!! CAGAAAATTTCAAATGTAAA+AGG - chr4_2:73786008-73786027 None:intergenic 20.0%
!!! ATTAATTAGGAGTATATGTT+TGG + chr4_2:73786301-73786320 Msa0621610:intron 20.0%
!!! TTAATTAGGAGTATATGTTT+GGG + chr4_2:73786302-73786321 Msa0621610:intron 20.0%
!! AATAAATGTATCTTGCAATT+TGG + chr4_2:73786331-73786350 Msa0621610:intron 20.0%
!!! AGAAAAACATTAGTTTTCTT+CGG + chr4_2:73786558-73786577 Msa0621610:intron 20.0%
!!! GATAAGTTATGATTTCTTTT+TGG + chr4_2:73786727-73786746 Msa0621610:intron 20.0%
!! CTATATAAATAACATGCTAA+AGG - chr4_2:73786916-73786935 None:intergenic 20.0%
!!! CTTTAGCATGTTATTTATAT+AGG + chr4_2:73786914-73786933 Msa0621610:three_prime_UTR 20.0%
!! TATTCAATACACTTTCTTTA+TGG + chr4_2:73786947-73786966 Msa0621610:three_prime_UTR 20.0%
!!! AAGAATTGTACGTTTGTTTT+GGG - chr4_2:73784182-73784201 None:intergenic 25.0%
!! AAAGGCACAAAAACAAAAAA+GGG - chr4_2:73784330-73784349 None:intergenic 25.0%
! AAAGAGAATGATTTGAAGAA+AGG - chr4_2:73784348-73784367 None:intergenic 25.0%
! AGAGAGTGATAAAAACAAAA+GGG - chr4_2:73784383-73784402 None:intergenic 25.0%
! TAGAGAGTGATAAAAACAAA+AGG - chr4_2:73784384-73784403 None:intergenic 25.0%
!! GAATGAAAGTTAAGATTTTG+AGG - chr4_2:73784420-73784439 None:intergenic 25.0%
! TAGAAGCAAAATTAGGAAAA+GGG - chr4_2:73784450-73784469 None:intergenic 25.0%
!! TTTTCCTAATTTTGCTTCTA+GGG + chr4_2:73784450-73784469 Msa0621610:five_prime_UTR 25.0%
! GAAAGATGTAATGAAGAAAA+AGG - chr4_2:73785540-73785559 None:intergenic 25.0%
! ATACTTACTGTAATTCTTGT+AGG + chr4_2:73785624-73785643 Msa0621610:intron 25.0%
! AACAATGCATAATATATGCA+AGG - chr4_2:73785907-73785926 None:intergenic 25.0%
!! GAATTGTATGTTATTTGTCA+CGG + chr4_2:73785977-73785996 Msa0621610:intron 25.0%
! TATTATCTTTCCTAAAATGG+CGG + chr4_2:73786098-73786117 Msa0621610:intron 25.0%
! GTGCTTATAATAAGTAACAA+AGG - chr4_2:73786134-73786153 None:intergenic 25.0%
!! TAATTAGGAGTATATGTTTG+GGG + chr4_2:73786303-73786322 Msa0621610:intron 25.0%
!!! GACATTCTTTTTTTACGAAA+AGG + chr4_2:73786373-73786392 Msa0621610:intron 25.0%
! ACTAAAAAAACAACTTACAC+AGG - chr4_2:73786537-73786556 None:intergenic 25.0%
! AATAACAAATGAGTAGTGAA+AGG - chr4_2:73786628-73786647 None:intergenic 25.0%
!!! ATTTGTTATTTTTAGCACTG+TGG + chr4_2:73786638-73786657 Msa0621610:intron 25.0%
! ATTATCATTGTTCAGATCAA+AGG + chr4_2:73786856-73786875 Msa0621610:CDS 25.0%
!! ATTTAAGTTCATACATGAGA+CGG - chr4_2:73786883-73786902 None:intergenic 25.0%
!! ATTGTTACTTAAATCACCAA+TGG - chr4_2:73786986-73787005 None:intergenic 25.0%
! GAAGAATTGTACGTTTGTTT+TGG - chr4_2:73784183-73784202 None:intergenic 30.0%
AAGGCACAAAAACAAAAAAG+GGG - chr4_2:73784329-73784348 None:intergenic 30.0%
! GAAAGGCACAAAAACAAAAA+AGG - chr4_2:73784331-73784350 None:intergenic 30.0%
CTAGAAGCAAAATTAGGAAA+AGG - chr4_2:73784451-73784470 None:intergenic 30.0%
! CTTTTCCTAATTTTGCTTCT+AGG + chr4_2:73784449-73784468 Msa0621610:five_prime_UTR 30.0%
!! ACAATCTTGGTTTTCCTTAT+CGG - chr4_2:73784801-73784820 None:intergenic 30.0%
! ACCAAGATTGTTATTGTGTT+AGG + chr4_2:73784810-73784829 Msa0621610:CDS 30.0%
TCCTAACACAATAACAATCT+TGG - chr4_2:73784814-73784833 None:intergenic 30.0%
AATCCTAGTGTTCATTTCTT+TGG + chr4_2:73784848-73784867 Msa0621610:CDS 30.0%
!! CATTTCTTTGGTGTTTATGA+TGG + chr4_2:73784860-73784879 Msa0621610:CDS 30.0%
TAATGATTCTGCATTGTTAG+AGG + chr4_2:73784946-73784965 Msa0621610:CDS 30.0%
!!! GGCTTATAATTCAGCTTTTT+TGG + chr4_2:73784979-73784998 Msa0621610:CDS 30.0%
TCATATTCATCTCTCCTAAA+TGG - chr4_2:73785184-73785203 None:intergenic 30.0%
ATACCATATCAACAACAGTT+TGG - chr4_2:73785495-73785514 None:intergenic 30.0%
TATGCTGATATTGTCTTCAT+TGG + chr4_2:73785591-73785610 Msa0621610:intron 30.0%
!! CTACATTTTCTTTTTTGTGG+TGG + chr4_2:73785862-73785881 Msa0621610:intron 30.0%
CATAATATATGCAAGGTTCA+GGG - chr4_2:73785900-73785919 None:intergenic 30.0%
!! GGAAAGGCATTTATTACATT+TGG + chr4_2:73785952-73785971 Msa0621610:intron 30.0%
! AAGGCATTTATTACATTTGG+TGG + chr4_2:73785955-73785974 Msa0621610:intron 30.0%
GATAATATATGGGAAGAATG+TGG - chr4_2:73786085-73786104 None:intergenic 30.0%
ACACACTTAATAGATGTGAA+AGG - chr4_2:73786162-73786181 None:intergenic 30.0%
AAGTAGGAACATTGGTAAAA+TGG - chr4_2:73786185-73786204 None:intergenic 30.0%
TGTATCTTGCAATTTGGAAA+AGG + chr4_2:73786337-73786356 Msa0621610:intron 30.0%
TTGGAAAAGGATTTATAGCT+AGG + chr4_2:73786350-73786369 Msa0621610:intron 30.0%
TGGAAAAGGATTTATAGCTA+GGG + chr4_2:73786351-73786370 Msa0621610:intron 30.0%
TACGAAAAGGTCATATACTA+AGG + chr4_2:73786386-73786405 Msa0621610:intron 30.0%
ACGAAAAGGTCATATACTAA+GGG + chr4_2:73786387-73786406 Msa0621610:intron 30.0%
! GTGCTACTAAATATGATGTT+TGG + chr4_2:73786660-73786679 Msa0621610:intron 30.0%
!!! ATGATTTCTTTTTGGAGTGT+AGG + chr4_2:73786735-73786754 Msa0621610:intron 30.0%
! TTTAAGTTCATACATGAGAC+GGG - chr4_2:73786882-73786901 None:intergenic 30.0%
GAAATGCACAAAGAAGGAAA+AGG - chr4_2:73784274-73784293 None:intergenic 35.0%
CAAACCCTAGAAGCAAAATT+AGG - chr4_2:73784457-73784476 None:intergenic 35.0%
!! TGTTCTTTGAAGTACAATGC+TGG + chr4_2:73784481-73784500 Msa0621610:five_prime_UTR 35.0%
!! GTTCTTTGAAGTACAATGCT+GGG + chr4_2:73784482-73784501 Msa0621610:five_prime_UTR 35.0%
! TCAAATATGGGTTGTGTTCA+TGG + chr4_2:73784599-73784618 Msa0621610:exon 35.0%
TGCTTCACACAATTTCACTA+TGG + chr4_2:73784733-73784752 Msa0621610:CDS 35.0%
!! CAATCTTGGTTTTCCTTATC+GGG - chr4_2:73784800-73784819 None:intergenic 35.0%
ACACCAAAGAAATGAACACT+AGG - chr4_2:73784854-73784873 None:intergenic 35.0%
!! TTTGGTGTTTATGATGGACA+TGG + chr4_2:73784866-73784885 Msa0621610:CDS 35.0%
!! TATGATGGACATGGTGAATT+TGG + chr4_2:73784875-73784894 Msa0621610:CDS 35.0%
ATTGTTAGAGGATCCTGTTA+AGG + chr4_2:73784958-73784977 Msa0621610:CDS 35.0%
GCTGAATTATAAGCCTTAAC+AGG - chr4_2:73784974-73784993 None:intergenic 35.0%
! GAGATTGATGATTGTATGAG+TGG + chr4_2:73785028-73785047 Msa0621610:CDS 35.0%
!! TGAGACTATTGGTGTTATTG+CGG + chr4_2:73785384-73785403 Msa0621610:CDS 35.0%
ACGACAAAGAAAAGATGATC+CGG - chr4_2:73785439-73785458 None:intergenic 35.0%
AAGCCAAACTGTTGTTGATA+TGG + chr4_2:73785489-73785508 Msa0621610:CDS 35.0%
!! ATTTTCTTTTTTGTGGTGGC+CGG + chr4_2:73785866-73785885 Msa0621610:intron 35.0%
GCATAATATATGCAAGGTTC+AGG - chr4_2:73785901-73785920 None:intergenic 35.0%
!! CAAGAATAAACCGCCATTTT+AGG - chr4_2:73786111-73786130 None:intergenic 35.0%
AACTTAGGAAGTAGGAACAT+TGG - chr4_2:73786193-73786212 None:intergenic 35.0%
TAAAGGGTAACTTAGGAAGT+AGG - chr4_2:73786201-73786220 None:intergenic 35.0%
TACTTCCTAAGTTACCCTTT+AGG + chr4_2:73786200-73786219 Msa0621610:intron 35.0%
CATTACCTAAAGGGTAACTT+AGG - chr4_2:73786208-73786227 None:intergenic 35.0%
TCACAAGACCAAATATCACT+AGG + chr4_2:73786475-73786494 Msa0621610:intron 35.0%
GATTAGCACCTAGTGATATT+TGG - chr4_2:73786486-73786505 None:intergenic 35.0%
! CACAGGTTGTGAAAACATTT+TGG - chr4_2:73786520-73786539 None:intergenic 35.0%
AGAGTCTTACAAACTATGGT+TGG + chr4_2:73786804-73786823 Msa0621610:CDS 35.0%
AAACTATGGTTGGAACATGA+GGG + chr4_2:73786814-73786833 Msa0621610:CDS 35.0%
AAGAGAGAAATTCAGAGGGA+TGG - chr4_2:73784116-73784135 None:intergenic 40.0%
ATCCCTCTGAATTTCTCTCT+TGG + chr4_2:73784115-73784134 Msa0621610:five_prime_UTR 40.0%
GTCCAAGAGAGAAATTCAGA+GGG - chr4_2:73784120-73784139 None:intergenic 40.0%
AGTCCAAGAGAGAAATTCAG+AGG - chr4_2:73784121-73784140 None:intergenic 40.0%
GGAAAAAAAAGCAGAACCCT+AGG - chr4_2:73784305-73784324 None:intergenic 40.0%
GCACAAAAACAAAAAAGGGG+TGG - chr4_2:73784326-73784345 None:intergenic 40.0%
GTGTGAGTATGTGCTTCTTA+GGG - chr4_2:73784688-73784707 None:intergenic 40.0%
TGTGTGAGTATGTGCTTCTT+AGG - chr4_2:73784689-73784708 None:intergenic 40.0%
! AGAAGCACATACTCACACAA+AGG + chr4_2:73784690-73784709 Msa0621610:CDS 40.0%
TACTCTGTACTTACTCAGAG+AGG + chr4_2:73784758-73784777 Msa0621610:CDS 40.0%
! TTTTCCTTATCGGGTGAATC+AGG - chr4_2:73784791-73784810 None:intergenic 40.0%
TATAACCAACACCGTAATCG+CGG - chr4_2:73785059-73785078 None:intergenic 40.0%
!! CGATTACGGTGTTGGTTATA+GGG + chr4_2:73785059-73785078 Msa0621610:CDS 40.0%
!! GATTACGGTGTTGGTTATAG+GGG + chr4_2:73785060-73785079 Msa0621610:CDS 40.0%
ACGCTTTATGTTGCGAATGT+TGG + chr4_2:73785085-73785104 Msa0621610:CDS 40.0%
TATGAGAGAGTGAAGCTTAG+TGG + chr4_2:73785199-73785218 Msa0621610:CDS 40.0%
AGGATCCTGATATTCAGACT+TGG + chr4_2:73785260-73785279 Msa0621610:CDS 40.0%
GGATCCTGATATTCAGACTT+GGG + chr4_2:73785261-73785280 Msa0621610:CDS 40.0%
GATCCTGATATTCAGACTTG+GGG + chr4_2:73785262-73785281 Msa0621610:CDS 40.0%
TGATGATCCTCCTAGATTGT+GGG + chr4_2:73785303-73785322 Msa0621610:CDS 40.0%
CTAGATTGTGGGTTCAGAAT+GGG + chr4_2:73785314-73785333 Msa0621610:CDS 40.0%
!! CTGCTTTTACAAGGAGTGTA+GGG + chr4_2:73785350-73785369 Msa0621610:CDS 40.0%
!! TGCTTTTACAAGGAGTGTAG+GGG + chr4_2:73785351-73785370 Msa0621610:CDS 40.0%
GATAGTTTGGCTGAGACTAT+TGG + chr4_2:73785373-73785392 Msa0621610:CDS 40.0%
TTCTTTGTCGTTGCAAGTGA+TGG + chr4_2:73785448-73785467 Msa0621610:CDS 40.0%
AAGAAAAAGGCTGCATTTGC+AGG - chr4_2:73785527-73785546 None:intergenic 40.0%
TTGTCAAATAGCAGCCATAG+CGG + chr4_2:73785675-73785694 Msa0621610:intron 40.0%
! AACCGCGGATTGCATAAAAT+GGG - chr4_2:73785826-73785845 None:intergenic 40.0%
!! TTTTCTTTTTTGTGGTGGCC+GGG + chr4_2:73785867-73785886 Msa0621610:intron 40.0%
TATATGCAAGGTTCAGGGTT+CGG - chr4_2:73785895-73785914 None:intergenic 40.0%
TTTGTAAGACTCTCCAGCAA+TGG - chr4_2:73786797-73786816 None:intergenic 40.0%
CTGGAGAGTCTTACAAACTA+TGG + chr4_2:73786800-73786819 Msa0621610:CDS 40.0%
CAAACTATGGTTGGAACATG+AGG + chr4_2:73786813-73786832 Msa0621610:CDS 40.0%
TGGAGAACTGAAAACTCCAT+TGG + chr4_2:73786967-73786986 Msa0621610:three_prime_UTR 40.0%
TTTGGGAGAGAGAGAATCGA+AGG - chr4_2:73784165-73784184 None:intergenic 45.0%
! TGGGGCTTTGTGAAAAGGAA+AGG - chr4_2:73784205-73784224 None:intergenic 45.0%
! TGAAGTGGGGCTTTGTGAAA+AGG - chr4_2:73784210-73784229 None:intergenic 45.0%
AGAAGAGAACGTGTGAAGTG+GGG - chr4_2:73784223-73784242 None:intergenic 45.0%
GAGAAGAGAACGTGTGAAGT+GGG - chr4_2:73784224-73784243 None:intergenic 45.0%
AGAGAAGAGAACGTGTGAAG+TGG - chr4_2:73784225-73784244 None:intergenic 45.0%
GCAGAGGAAATGCACAAAGA+AGG - chr4_2:73784280-73784299 None:intergenic 45.0%
TGCATTTCCTCTGCATCCTA+GGG + chr4_2:73784286-73784305 Msa0621610:five_prime_UTR 45.0%
CGTTATCCTGCACCATCAAT+TGG + chr4_2:73784635-73784654 Msa0621610:CDS 45.0%
TATCCTGCACCATCAATTGG+TGG + chr4_2:73784638-73784657 Msa0621610:CDS 45.0%
! ACCATCAATTGGTGGCTCTA+GGG + chr4_2:73784646-73784665 Msa0621610:CDS 45.0%
AGTATGTGCTTCTTAGGGAC+AGG - chr4_2:73784683-73784702 None:intergenic 45.0%
CTATCCTGATTCACCCGATA+AGG + chr4_2:73784784-73784803 Msa0621610:CDS 45.0%
TGTGTTAGGACGCAATTGCA+AGG + chr4_2:73784824-73784843 Msa0621610:CDS 45.0%
GATGGACATGGTGAATTTGG+TGG + chr4_2:73784878-73784897 Msa0621610:CDS 45.0%
!! GCGATTACGGTGTTGGTTAT+AGG + chr4_2:73785058-73785077 Msa0621610:CDS 45.0%
!! TTGCGAATGTTGGTGATTCG+AGG + chr4_2:73785095-73785114 Msa0621610:CDS 45.0%
!! TGCGAATGTTGGTGATTCGA+GGG + chr4_2:73785096-73785115 Msa0621610:CDS 45.0%
CGGGAATAGAATTGTAGCTC+AGG + chr4_2:73785138-73785157 Msa0621610:CDS 45.0%
CCTAAATGGTGTCTGATCAG+AGG - chr4_2:73785170-73785189 None:intergenic 45.0%
CCTCTGATCAGACACCATTT+AGG + chr4_2:73785167-73785186 Msa0621610:CDS 45.0%
! AGTGAAGCTTAGTGGTGCTA+GGG + chr4_2:73785207-73785226 Msa0621610:CDS 45.0%
!! TAGGGTGTTGAGTGTTGATC+AGG + chr4_2:73785225-73785244 Msa0621610:CDS 45.0%
!! TTGAGTGTTGATCAGGTGGA+AGG + chr4_2:73785232-73785251 Msa0621610:CDS 45.0%
!! TGAGTGTTGATCAGGTGGAA+GGG + chr4_2:73785233-73785252 Msa0621610:CDS 45.0%
TCACCCCAAGTCTGAATATC+AGG - chr4_2:73785268-73785287 None:intergenic 45.0%
GTGATGATCCTCCTAGATTG+TGG + chr4_2:73785302-73785321 Msa0621610:CDS 45.0%
TTCTGAACCCACAATCTAGG+AGG - chr4_2:73785313-73785332 None:intergenic 45.0%
CCATTCTGAACCCACAATCT+AGG - chr4_2:73785316-73785335 None:intergenic 45.0%
CCTAGATTGTGGGTTCAGAA+TGG + chr4_2:73785313-73785332 Msa0621610:CDS 45.0%
GTTCAGAATGGGATGATTCC+TGG + chr4_2:73785325-73785344 Msa0621610:CDS 45.0%
!!! TTCCTGGAGCTGCTTTTACA+AGG + chr4_2:73785341-73785360 Msa0621610:CDS 45.0%
!!! GCTGCTTTTACAAGGAGTGT+AGG + chr4_2:73785349-73785368 Msa0621610:CDS 45.0%
! AAGGAGTGTAGGGGATAGTT+TGG + chr4_2:73785360-73785379 Msa0621610:CDS 45.0%
GTTAACGGTTCAGCTTAAGC+CGG + chr4_2:73785417-73785436 Msa0621610:CDS 45.0%
!! CAACAACAGTTTGGCTTGAC+AGG - chr4_2:73785486-73785505 None:intergenic 45.0%
CGTGTATCGCAGAACAATAG+CGG - chr4_2:73785734-73785753 None:intergenic 45.0%
! TCCCCATTTTATGCAATCCG+CGG + chr4_2:73785821-73785840 Msa0621610:intron 45.0%
! ACCGCGGATTGCATAAAATG+GGG - chr4_2:73785825-73785844 None:intergenic 45.0%
! CAACCGCGGATTGCATAAAA+TGG - chr4_2:73785827-73785846 None:intergenic 45.0%
!!! TTTCTTTTTTGTGGTGGCCG+GGG + chr4_2:73785868-73785887 Msa0621610:intron 45.0%
GTGCATTTCCTCTGCATCCT+AGG + chr4_2:73784285-73784304 Msa0621610:five_prime_UTR 50.0%
! CACCATCAATTGGTGGCTCT+AGG + chr4_2:73784645-73784664 Msa0621610:CDS 50.0%
TCCCTAGAGCCACCAATTGA+TGG - chr4_2:73784650-73784669 None:intergenic 50.0%
GAGTGGTACTACCGCGATTA+CGG + chr4_2:73785045-73785064 Msa0621610:CDS 50.0%
TACTACCGCGATTACGGTGT+TGG + chr4_2:73785051-73785070 Msa0621610:CDS 50.0%
!! GAATGTTGGTGATTCGAGGG+CGG + chr4_2:73785099-73785118 Msa0621610:CDS 50.0%
GAGTGAAGCTTAGTGGTGCT+AGG + chr4_2:73785206-73785225 Msa0621610:CDS 50.0%
!! GGTGTTGAGTGTTGATCAGG+TGG + chr4_2:73785228-73785247 Msa0621610:CDS 50.0%
! TGATCAGGTGGAAGGGCATA+AGG + chr4_2:73785240-73785259 Msa0621610:CDS 50.0%
TTGGGGTGATGAAGAGAGTC+AGG + chr4_2:73785279-73785298 Msa0621610:CDS 50.0%
TGGGGTGATGAAGAGAGTCA+GGG + chr4_2:73785280-73785299 Msa0621610:CDS 50.0%
CTCCTTGTAAAAGCAGCTCC+AGG - chr4_2:73785346-73785365 None:intergenic 50.0%
!! TGGTGTTATTGCGGTTCCAG+AGG + chr4_2:73785393-73785412 Msa0621610:CDS 50.0%
!! TGCGGTTCCAGAGGTGTTAA+CGG + chr4_2:73785402-73785421 Msa0621610:CDS 50.0%
AGCTGAACCGTTAACACCTC+TGG - chr4_2:73785412-73785431 None:intergenic 50.0%
! GCGGATTGCATAAAATGGGG+AGG - chr4_2:73785822-73785841 None:intergenic 50.0%
AAATGCCTAGTCGTCAACCG+CGG - chr4_2:73785841-73785860 None:intergenic 50.0%
TGAGCTAAGCTCACGAGGAA+AGG + chr4_2:73785936-73785955 Msa0621610:intron 50.0%
AATGGCAGCACATGCATCAC+GGG - chr4_2:73786779-73786798 None:intergenic 50.0%
CAATGGCAGCACATGCATCA+CGG - chr4_2:73786780-73786799 None:intergenic 50.0%
AGCAGAACCCTAGGATGCAG+AGG - chr4_2:73784296-73784315 None:intergenic 55.0%
!! GAGCCACCAATTGATGGTGC+AGG - chr4_2:73784644-73784663 None:intergenic 55.0%
!! GCGGTGTTGGCTGTTAAGGA+CGG + chr4_2:73785118-73785137 Msa0621610:CDS 55.0%
!! CGGTGTTGGCTGTTAAGGAC+GGG + chr4_2:73785119-73785138 Msa0621610:CDS 55.0%
GCTATGCTATAGCGCCGCTA+TGG - chr4_2:73785692-73785711 None:intergenic 55.0%
CCTCGTGAGCTTAGCTCAGT+TGG - chr4_2:73785934-73785953 None:intergenic 55.0%
CCAACTGAGCTAAGCTCACG+AGG + chr4_2:73785931-73785950 Msa0621610:intron 55.0%
ATGGCAGCACATGCATCACG+GGG - chr4_2:73786778-73786797 None:intergenic 55.0%
GATGCATGTGCTGCCATTGC+TGG + chr4_2:73786781-73786800 Msa0621610:CDS 55.0%
TGGTGATTCGAGGGCGGTGT+TGG + chr4_2:73785105-73785124 Msa0621610:CDS 60.0%
!! GAGGGCGGTGTTGGCTGTTA+AGG + chr4_2:73785114-73785133 Msa0621610:CDS 60.0%
GCAATCCGCGGTTGACGACT+AGG + chr4_2:73785833-73785852 Msa0621610:intron 60.0%
AAGGTTCAGGGTTCGGACCC+CGG - chr4_2:73785888-73785907 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 73784099 73787017 73784099 ID=Msa0621610;Name=Msa0621610
chr4_2 mRNA 73784099 73787017 73784099 ID=Msa0621610-mRNA-1;Parent=Msa0621610;Name=Msa0621610-mRNA-1;_AED=0.15;_eAED=0.15;_QI=506|1|1|1|1|1|2|123|347
chr4_2 exon 73784099 73785510 73784099 ID=Msa0621610-mRNA-1:exon:22173;Parent=Msa0621610-mRNA-1
chr4_2 exon 73786757 73787017 73786757 ID=Msa0621610-mRNA-1:exon:22174;Parent=Msa0621610-mRNA-1
chr4_2 five_prime_UTR 73784099 73784604 73784099 ID=Msa0621610-mRNA-1:five_prime_utr;Parent=Msa0621610-mRNA-1
chr4_2 CDS 73784605 73785510 73784605 ID=Msa0621610-mRNA-1:cds;Parent=Msa0621610-mRNA-1
chr4_2 CDS 73786757 73786894 73786757 ID=Msa0621610-mRNA-1:cds;Parent=Msa0621610-mRNA-1
chr4_2 three_prime_UTR 73786895 73787017 73786895 ID=Msa0621610-mRNA-1:three_prime_utr;Parent=Msa0621610-mRNA-1
Gene Sequence

>Msa0621610

ATGGGTTGTGTTCATGGAAAGTGTTGTAGTCGTTATCCTGCACCATCAATTGGTGGCTCTAGGGACTATAGAGAACCTGTCCCTAAGAAGCACATACTCACACAAAGGTCACTTCACTTTGTTGATGTTGCTTCACACAATTTCACTATGGAATACTCTGTACTTACTCAGAGAGGTTACTATCCTGATTCACCCGATAAGGAAAACCAAGATTGTTATTGTGTTAGGACGCAATTGCAAGGTAATCCTAGTGTTCATTTCTTTGGTGTTTATGATGGACATGGTGAATTTGGTGGACTGTGTTCTAATTTTGTTAAGAATAAGTTGGTTGAAAAATTGTCTAATGATTCTGCATTGTTAGAGGATCCTGTTAAGGCTTATAATTCAGCTTTTTTGGCTACGAATGATGAGTTGCATAAGAGTGAGATTGATGATTGTATGAGTGGTACTACCGCGATTACGGTGTTGGTTATAGGGGATACGCTTTATGTTGCGAATGTTGGTGATTCGAGGGCGGTGTTGGCTGTTAAGGACGGGAATAGAATTGTAGCTCAGGATTTGTCCTCTGATCAGACACCATTTAGGAGAGATGAATATGAGAGAGTGAAGCTTAGTGGTGCTAGGGTGTTGAGTGTTGATCAGGTGGAAGGGCATAAGGATCCTGATATTCAGACTTGGGGTGATGAAGAGAGTCAGGGTGATGATCCTCCTAGATTGTGGGTTCAGAATGGGATGATTCCTGGAGCTGCTTTTACAAGGAGTGTAGGGGATAGTTTGGCTGAGACTATTGGTGTTATTGCGGTTCCAGAGGTGTTAACGGTTCAGCTTAAGCCGGATCATCTTTTCTTTGTCGTTGCAAGTGATGGTGTATTTGAGTTCCTGTCAAGCCAAACTGTTGTTGATATGGCTGCAAGTTATTCAGATCCCCGTGATGCATGTGCTGCCATTGCTGGAGAGTCTTACAAACTATGGTTGGAACATGAGGGTCGAACAGATGATATAACAATTATCATTGTTCAGATCAAAGGCCCGTCTCATGTATGA
Protein sequence

>Msa0621610

MGCVHGKCCSRYPAPSIGGSRDYREPVPKKHILTQRSLHFVDVASHNFTMEYSVLTQRGYYPDSPDKENQDCYCVRTQLQGNPSVHFFGVYDGHGEFGGLCSNFVKNKLVEKLSNDSALLEDPVKAYNSAFLATNDELHKSEIDDCMSGTTAITVLVIGDTLYVANVGDSRAVLAVKDGNRIVAQDLSSDQTPFRRDEYERVKLSGARVLSVDQVEGHKDPDIQTWGDEESQGDDPPRLWVQNGMIPGAAFTRSVGDSLAETIGVIAVPEVLTVQLKPDHLFFVVASDGVFEFLSSQTVVDMAASYSDPRDACAAIAGESYKLWLEHEGRTDDITIIIVQIKGPSHV*