AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0772020


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0772020 MtrunA17_Chr5g0425991 95.305 213 8 2 1 211 1 213 4.23e-146 405
Msa0772020 MtrunA17_Chr3g0115791 57.485 167 43 9 63 210 2 159 4.76e-47 152
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0772020 AT1G78170.1 37.500 208 90 8 19 203 22 212 2.41e-26 101
Msa0772020 AT1G22250.1 42.609 115 52 3 110 210 85 199 4.66e-16 73.9
Msa0772020 AT4G08910.1 41.071 112 43 2 107 195 86 197 6.00e-16 73.6

Find 36 sgRNAs with CRISPR-Local

Find 113 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATCACATAGACTTATTTAT+TGG 0.127321 5_2:-45938620 None:intergenic
TTCTGATCAAGAATTCTTTA+AGG 0.147055 5_2:+45937837 Msa0772020:CDS
CACCTTTGCCTTCAGATAAA+TGG 0.203598 5_2:+45937920 Msa0772020:CDS
TTAAGAACATACATAAATTT+AGG 0.233603 5_2:-45938829 None:intergenic
CTTCACAAAAGAAGAGAAAA+TGG 0.266395 5_2:+45937803 Msa0772020:CDS
AAACCACTCTTGTTTGAATA+TGG 0.304175 5_2:+45937720 Msa0772020:exon
AAAATTTGTTCTCACTATAC+TGG 0.306519 5_2:-45938912 None:intergenic
ATCAATTAGCAAGAATAAAA+AGG 0.319819 5_2:+45938372 Msa0772020:CDS
TATTATATATTTCAAACAAA+AGG 0.340498 5_2:+45938669 Msa0772020:three_prime_UTR
CAAGAACAACCACTTCTAAT+TGG 0.345178 5_2:+45938596 Msa0772020:three_prime_UTR
AAGGATCTTACACATGATCT+TGG 0.370919 5_2:+45937856 Msa0772020:CDS
GGATGCATGAATTTGCAATT+AGG 0.374086 5_2:-45938511 None:intergenic
GTCAAATTACAACTCCTTAG+AGG 0.386148 5_2:-45938713 None:intergenic
GTATGTTTCTTAAAGTTGTA+AGG 0.389476 5_2:+45938859 Msa0772020:three_prime_UTR
GCAACAATACCTCAATGTTC+AGG 0.426385 5_2:+45937942 Msa0772020:CDS
TTAATCTACCTCTACCTCTA+AGG 0.434008 5_2:+45938699 Msa0772020:three_prime_UTR
AGGATCTTACACATGATCTT+GGG 0.444226 5_2:+45937857 Msa0772020:CDS
ATTATATATTTCAAACAAAA+GGG 0.459409 5_2:+45938670 Msa0772020:three_prime_UTR
AATTTGGAAGGGTTCTGATC+TGG 0.466878 5_2:-45938538 None:intergenic
GAAGGGTTCTGATCTGGTGG+TGG 0.491999 5_2:-45938532 None:intergenic
GTGAAGTTCTATATCAAAGA+TGG 0.507333 5_2:-45937890 None:intergenic
CTAAGGAGTTGTAATTTGAC+TGG 0.508158 5_2:+45938716 Msa0772020:three_prime_UTR
TTCACAAAAGAAGAGAAAAT+GGG 0.522716 5_2:+45937804 Msa0772020:CDS
TTACAACTCCTTAGAGGTAG+AGG 0.525637 5_2:-45938707 None:intergenic
CACATGTCAAATTAAGCTCA+AGG 0.527855 5_2:-45938291 None:intergenic
TATTGTTGCCATTTATCTGA+AGG 0.533510 5_2:-45937928 None:intergenic
TGTGAAGCATCAAAATCTGT+AGG 0.557840 5_2:-45937787 None:intergenic
AATTTGCAATTAGGACAAGA+AGG 0.564845 5_2:-45938502 None:intergenic
TTTATTGGACCAATTAGAAG+TGG 0.571145 5_2:-45938605 None:intergenic
TTGGAAGGGTTCTGATCTGG+TGG 0.597114 5_2:-45938535 None:intergenic
TGCCATTTATCTGAAGGCAA+AGG 0.597180 5_2:-45937922 None:intergenic
TGGTTATCATCATCATCAGA+TGG 0.614533 5_2:+45938406 Msa0772020:CDS
TATTATATACCTGAACATTG+AGG 0.617071 5_2:-45937951 None:intergenic
TGACCATATTCAAACAAGAG+TGG 0.637366 5_2:-45937723 None:intergenic
TTGTATGAAGTGTCACATGT+TGG 0.683627 5_2:+45938459 Msa0772020:CDS
TATGATGATTATGATACACA+AGG 0.728153 5_2:-45938772 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATTATATATTTCAAACAAA+AGG + chr5_2:45938669-45938688 Msa0772020:three_prime_UTR 10.0%
!! ATTATATATTTCAAACAAAA+GGG + chr5_2:45938670-45938689 Msa0772020:three_prime_UTR 10.0%
!! TATTATATATTTCAAACAAA+AGG + chr5_2:45938669-45938688 Msa0772020:three_prime_UTR 10.0%
!! ATTATATATTTCAAACAAAA+GGG + chr5_2:45938670-45938689 Msa0772020:three_prime_UTR 10.0%
!! AAAATGAAATATATTAACCT+CGG - chr5_2:45938030-45938049 None:intergenic 15.0%
!! TTAAGAACATACATAAATTT+AGG - chr5_2:45938832-45938851 None:intergenic 15.0%
!! AAAATGAAATATATTAACCT+CGG - chr5_2:45938030-45938049 None:intergenic 15.0%
!! TTAAGAACATACATAAATTT+AGG - chr5_2:45938832-45938851 None:intergenic 15.0%
!! ATAAAATATTATTGCTACCT+CGG - chr5_2:45938076-45938095 None:intergenic 20.0%
!! ATCAATTAGCAAGAATAAAA+AGG + chr5_2:45938372-45938391 Msa0772020:CDS 20.0%
!! AATCACATAGACTTATTTAT+TGG - chr5_2:45938623-45938642 None:intergenic 20.0%
!! ATAAAATATTATTGCTACCT+CGG - chr5_2:45938076-45938095 None:intergenic 20.0%
!! ATCAATTAGCAAGAATAAAA+AGG + chr5_2:45938372-45938391 Msa0772020:CDS 20.0%
!! AATCACATAGACTTATTTAT+TGG - chr5_2:45938623-45938642 None:intergenic 20.0%
! TTCACAAAAGAAGAGAAAAT+GGG + chr5_2:45937804-45937823 Msa0772020:CDS 25.0%
! TTCTGATCAAGAATTCTTTA+AGG + chr5_2:45937837-45937856 Msa0772020:CDS 25.0%
! TATTATATACCTGAACATTG+AGG - chr5_2:45937954-45937973 None:intergenic 25.0%
! CGAAAATGTGCTTATAAAAA+GGG - chr5_2:45937986-45938005 None:intergenic 25.0%
! ATAAAAAGGATTCTTCATCA+TGG + chr5_2:45938386-45938405 Msa0772020:CDS 25.0%
! TTCTCTTCAAAAATTTGGAA+GGG - chr5_2:45938552-45938571 None:intergenic 25.0%
! TATGATGATTATGATACACA+AGG - chr5_2:45938775-45938794 None:intergenic 25.0%
! GTATGTTTCTTAAAGTTGTA+AGG + chr5_2:45938859-45938878 Msa0772020:three_prime_UTR 25.0%
! TTCACAAAAGAAGAGAAAAT+GGG + chr5_2:45937804-45937823 Msa0772020:CDS 25.0%
! TTCTGATCAAGAATTCTTTA+AGG + chr5_2:45937837-45937856 Msa0772020:CDS 25.0%
! TATTATATACCTGAACATTG+AGG - chr5_2:45937954-45937973 None:intergenic 25.0%
! CGAAAATGTGCTTATAAAAA+GGG - chr5_2:45937986-45938005 None:intergenic 25.0%
! ATAAAAAGGATTCTTCATCA+TGG + chr5_2:45938386-45938405 Msa0772020:CDS 25.0%
! TTCTCTTCAAAAATTTGGAA+GGG - chr5_2:45938552-45938571 None:intergenic 25.0%
! TATGATGATTATGATACACA+AGG - chr5_2:45938775-45938794 None:intergenic 25.0%
! GTATGTTTCTTAAAGTTGTA+AGG + chr5_2:45938859-45938878 Msa0772020:three_prime_UTR 25.0%
! AAACCACTCTTGTTTGAATA+TGG + chr5_2:45937720-45937739 Msa0772020:exon 30.0%
!!! AGATTCTTTTTTTGGACTCA+TGG - chr5_2:45937767-45937786 None:intergenic 30.0%
!!! TCTGTAGGAGATTCTTTTTT+TGG - chr5_2:45937775-45937794 None:intergenic 30.0%
CTTCACAAAAGAAGAGAAAA+TGG + chr5_2:45937803-45937822 Msa0772020:CDS 30.0%
GTGAAGTTCTATATCAAAGA+TGG - chr5_2:45937893-45937912 None:intergenic 30.0%
! TATTGTTGCCATTTATCTGA+AGG - chr5_2:45937931-45937950 None:intergenic 30.0%
AATGTGCTTATAAAAAGGGA+CGG - chr5_2:45937982-45938001 None:intergenic 30.0%
GCGAAAATGTGCTTATAAAA+AGG - chr5_2:45937987-45938006 None:intergenic 30.0%
! TTAAATTTTCTCATGCAGTC+AGG + chr5_2:45938166-45938185 Msa0772020:intron 30.0%
! TAAATTTTCTCATGCAGTCA+GGG + chr5_2:45938167-45938186 Msa0772020:intron 30.0%
!!! GAGATGATTTTTCAGGTAAA+GGG - chr5_2:45938252-45938271 None:intergenic 30.0%
!!! AGAGATGATTTTTCAGGTAA+AGG - chr5_2:45938253-45938272 None:intergenic 30.0%
TTACCTGAAAAATCATCTCT+TGG + chr5_2:45938253-45938272 Msa0772020:CDS 30.0%
AATTTGCAATTAGGACAAGA+AGG - chr5_2:45938505-45938524 None:intergenic 30.0%
GTTCTCTTCAAAAATTTGGA+AGG - chr5_2:45938553-45938572 None:intergenic 30.0%
CCTTGTTCTCTTCAAAAATT+TGG - chr5_2:45938557-45938576 None:intergenic 30.0%
!! CCAAATTTTTGAAGAGAACA+AGG + chr5_2:45938554-45938573 Msa0772020:CDS 30.0%
TTTATTGGACCAATTAGAAG+TGG - chr5_2:45938608-45938627 None:intergenic 30.0%
!! TTTTATTTGCCAGTATAGTG+AGG + chr5_2:45938903-45938922 Msa0772020:three_prime_UTR 30.0%
! AAACCACTCTTGTTTGAATA+TGG + chr5_2:45937720-45937739 Msa0772020:exon 30.0%
!!! AGATTCTTTTTTTGGACTCA+TGG - chr5_2:45937767-45937786 None:intergenic 30.0%
!!! TCTGTAGGAGATTCTTTTTT+TGG - chr5_2:45937775-45937794 None:intergenic 30.0%
CTTCACAAAAGAAGAGAAAA+TGG + chr5_2:45937803-45937822 Msa0772020:CDS 30.0%
GTGAAGTTCTATATCAAAGA+TGG - chr5_2:45937893-45937912 None:intergenic 30.0%
! TATTGTTGCCATTTATCTGA+AGG - chr5_2:45937931-45937950 None:intergenic 30.0%
AATGTGCTTATAAAAAGGGA+CGG - chr5_2:45937982-45938001 None:intergenic 30.0%
GCGAAAATGTGCTTATAAAA+AGG - chr5_2:45937987-45938006 None:intergenic 30.0%
! TTAAATTTTCTCATGCAGTC+AGG + chr5_2:45938166-45938185 Msa0772020:intron 30.0%
! TAAATTTTCTCATGCAGTCA+GGG + chr5_2:45938167-45938186 Msa0772020:intron 30.0%
!!! GAGATGATTTTTCAGGTAAA+GGG - chr5_2:45938252-45938271 None:intergenic 30.0%
!!! AGAGATGATTTTTCAGGTAA+AGG - chr5_2:45938253-45938272 None:intergenic 30.0%
TTACCTGAAAAATCATCTCT+TGG + chr5_2:45938253-45938272 Msa0772020:CDS 30.0%
AATTTGCAATTAGGACAAGA+AGG - chr5_2:45938505-45938524 None:intergenic 30.0%
GTTCTCTTCAAAAATTTGGA+AGG - chr5_2:45938553-45938572 None:intergenic 30.0%
CCTTGTTCTCTTCAAAAATT+TGG - chr5_2:45938557-45938576 None:intergenic 30.0%
!! CCAAATTTTTGAAGAGAACA+AGG + chr5_2:45938554-45938573 Msa0772020:CDS 30.0%
TTTATTGGACCAATTAGAAG+TGG - chr5_2:45938608-45938627 None:intergenic 30.0%
TGACCATATTCAAACAAGAG+TGG - chr5_2:45937726-45937745 None:intergenic 35.0%
! TGTGAAGCATCAAAATCTGT+AGG - chr5_2:45937790-45937809 None:intergenic 35.0%
AAGGATCTTACACATGATCT+TGG + chr5_2:45937856-45937875 Msa0772020:CDS 35.0%
AGGATCTTACACATGATCTT+GGG + chr5_2:45937857-45937876 Msa0772020:CDS 35.0%
!! CTAGTTTTTAATTAGAGCCG+AGG + chr5_2:45938010-45938029 Msa0772020:intron 35.0%
TTCAAAACAAATTAGAGCCG+AGG + chr5_2:45938056-45938075 Msa0772020:intron 35.0%
! TGACCAAGAGATGATTTTTC+AGG - chr5_2:45938259-45938278 None:intergenic 35.0%
CACATGTCAAATTAAGCTCA+AGG - chr5_2:45938294-45938313 None:intergenic 35.0%
TGGTTATCATCATCATCAGA+TGG + chr5_2:45938406-45938425 Msa0772020:CDS 35.0%
! TTGTATGAAGTGTCACATGT+TGG + chr5_2:45938459-45938478 Msa0772020:CDS 35.0%
GGATGCATGAATTTGCAATT+AGG - chr5_2:45938514-45938533 None:intergenic 35.0%
CAAGAACAACCACTTCTAAT+TGG + chr5_2:45938596-45938615 Msa0772020:three_prime_UTR 35.0%
TTAATCTACCTCTACCTCTA+AGG + chr5_2:45938699-45938718 Msa0772020:three_prime_UTR 35.0%
GTCAAATTACAACTCCTTAG+AGG - chr5_2:45938716-45938735 None:intergenic 35.0%
CTAAGGAGTTGTAATTTGAC+TGG + chr5_2:45938716-45938735 Msa0772020:three_prime_UTR 35.0%
TGACCATATTCAAACAAGAG+TGG - chr5_2:45937726-45937745 None:intergenic 35.0%
! TGTGAAGCATCAAAATCTGT+AGG - chr5_2:45937790-45937809 None:intergenic 35.0%
AAGGATCTTACACATGATCT+TGG + chr5_2:45937856-45937875 Msa0772020:CDS 35.0%
AGGATCTTACACATGATCTT+GGG + chr5_2:45937857-45937876 Msa0772020:CDS 35.0%
!! CTAGTTTTTAATTAGAGCCG+AGG + chr5_2:45938010-45938029 Msa0772020:intron 35.0%
TTCAAAACAAATTAGAGCCG+AGG + chr5_2:45938056-45938075 Msa0772020:intron 35.0%
! TGACCAAGAGATGATTTTTC+AGG - chr5_2:45938259-45938278 None:intergenic 35.0%
CACATGTCAAATTAAGCTCA+AGG - chr5_2:45938294-45938313 None:intergenic 35.0%
TGGTTATCATCATCATCAGA+TGG + chr5_2:45938406-45938425 Msa0772020:CDS 35.0%
! TTGTATGAAGTGTCACATGT+TGG + chr5_2:45938459-45938478 Msa0772020:CDS 35.0%
GGATGCATGAATTTGCAATT+AGG - chr5_2:45938514-45938533 None:intergenic 35.0%
CAAGAACAACCACTTCTAAT+TGG + chr5_2:45938596-45938615 Msa0772020:three_prime_UTR 35.0%
TTAATCTACCTCTACCTCTA+AGG + chr5_2:45938699-45938718 Msa0772020:three_prime_UTR 35.0%
GTCAAATTACAACTCCTTAG+AGG - chr5_2:45938716-45938735 None:intergenic 35.0%
CTAAGGAGTTGTAATTTGAC+TGG + chr5_2:45938716-45938735 Msa0772020:three_prime_UTR 35.0%
CACCTTTGCCTTCAGATAAA+TGG + chr5_2:45937920-45937939 Msa0772020:CDS 40.0%
! TGCCATTTATCTGAAGGCAA+AGG - chr5_2:45937925-45937944 None:intergenic 40.0%
GCAACAATACCTCAATGTTC+AGG + chr5_2:45937942-45937961 Msa0772020:CDS 40.0%
! AATTTGGAAGGGTTCTGATC+TGG - chr5_2:45938541-45938560 None:intergenic 40.0%
TTACAACTCCTTAGAGGTAG+AGG - chr5_2:45938710-45938729 None:intergenic 40.0%
CACCTTTGCCTTCAGATAAA+TGG + chr5_2:45937920-45937939 Msa0772020:CDS 40.0%
! TGCCATTTATCTGAAGGCAA+AGG - chr5_2:45937925-45937944 None:intergenic 40.0%
GCAACAATACCTCAATGTTC+AGG + chr5_2:45937942-45937961 Msa0772020:CDS 40.0%
! AATTTGGAAGGGTTCTGATC+TGG - chr5_2:45938541-45938560 None:intergenic 40.0%
TTACAACTCCTTAGAGGTAG+AGG - chr5_2:45938710-45938729 None:intergenic 40.0%
!!! CTGAAGGCAAAGGTGTTTTG+AGG - chr5_2:45937915-45937934 None:intergenic 45.0%
!!! CTGAAGGCAAAGGTGTTTTG+AGG - chr5_2:45937915-45937934 None:intergenic 45.0%
! TTGGAAGGGTTCTGATCTGG+TGG - chr5_2:45938538-45938557 None:intergenic 50.0%
! TTGGAAGGGTTCTGATCTGG+TGG - chr5_2:45938538-45938557 None:intergenic 50.0%
! GAAGGGTTCTGATCTGGTGG+TGG - chr5_2:45938535-45938554 None:intergenic 55.0%
! GAAGGGTTCTGATCTGGTGG+TGG - chr5_2:45938535-45938554 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr5_2 gene 45937703 45938924 45937703 ID=Msa0772020;Name=Msa0772020
chr5_2 mRNA 45937703 45938924 45937703 ID=Msa0772020-mRNA-1;Parent=Msa0772020;Name=Msa0772020-mRNA-1;_AED=0.02;_eAED=0.02;_QI=36|1|1|1|1|1|2|330|211
chr5_2 exon 45937703 45937963 45937703 ID=Msa0772020-mRNA-1:exon:13781;Parent=Msa0772020-mRNA-1
chr5_2 exon 45938184 45938924 45938184 ID=Msa0772020-mRNA-1:exon:13782;Parent=Msa0772020-mRNA-1
chr5_2 five_prime_UTR 45937703 45937738 45937703 ID=Msa0772020-mRNA-1:five_prime_utr;Parent=Msa0772020-mRNA-1
chr5_2 CDS 45937739 45937963 45937739 ID=Msa0772020-mRNA-1:cds;Parent=Msa0772020-mRNA-1
chr5_2 CDS 45938184 45938594 45938184 ID=Msa0772020-mRNA-1:cds;Parent=Msa0772020-mRNA-1
chr5_2 three_prime_UTR 45938595 45938924 45938595 ID=Msa0772020-mRNA-1:three_prime_utr;Parent=Msa0772020-mRNA-1
Gene Sequence

>Msa0772020

ATGGTCACACTTCACAAAGCACTAGCCATGAGTCCAAAAAAAGAATCTCCTACAGATTTTGATGCTTCACAAAAGAAGAGAAAATGGGAAGAATCACTTTCTGATCAAGAATTCTTTAAGGATCTTACACATGATCTTGGGAAAAGAAAATCCATCTTTGATATAGAACTTCACCTCAAAACACCTTTGCCTTCAGATAAATGGCAACAATACCTCAATGTTCAGTCAGGGCAAATACAATTGTGTAACACAAAGATGAATAAGAAAACACAAGAATCAAAAAGAGAGTACCCTTTACCTGAAAAATCATCTCTTGGTCATTTAAGCTTAGACCTTGAGCTTAATTTGACATGTGAAACATTGAAGAAAAAAGAAGAAAGTTATTTTGAAAAGAAGAATAATCTAGTGAATGAATCAATTAGCAAGAATAAAAAGGATTCTTCATCATGGTTATCATCATCATCAGATGGTGATGATTACAAAGAAATGATTGCAACAGTTTGTATGAAGTGTCACATGTTGGTAATGCTTTGCAAATCATCTCCTTCTTGTCCTAATTGCAAATTCATGCATCCACCACCAGATCAGAACCCTTCCAAATTTTTGAAGAGAACAAGGTTTAGTTTCTTGACATAA

Protein sequence

>Msa0772020

MVTLHKALAMSPKKESPTDFDASQKKRKWEESLSDQEFFKDLTHDLGKRKSIFDIELHLKTPLPSDKWQQYLNVQSGQIQLCNTKMNKKTQESKREYPLPEKSSLGHLSLDLELNLTCETLKKKEESYFEKKNNLVNESISKNKKDSSSWLSSSSDGDDYKEMIATVCMKCHMLVMLCKSSPSCPNCKFMHPPPDQNPSKFLKRTRFSFLT*