AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0782760

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0782760 MtrunA17_Chr5g0440321 94.944 178 7 2 176 353 102 277 4.70e-114 332
Msa0782760 MtrunA17_Chr5g0440321 85.185 162 16 2 1 154 1 162 1.71e-91 275
Msa0782760 MtrunA17_Chr5g0440311 34.483 348 139 13 1 342 1 265 5.17e-34 126
Msa0782760 MtrunA17_Chr5g0440291 44.545 110 58 2 1 108 3 111 4.48e-25 102
Msa0782760 MtrunA17_Chr6g0481571 32.824 131 80 3 1 128 1 126 5.13e-14 69.3
Msa0782760 MtrunA17_Chr8g0356011 29.075 227 131 8 4 226 15 215 1.61e-13 71.2
Msa0782760 MtrunA17_Chr7g0253661 37.209 86 52 1 10 93 8 93 2.00e-12 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0782760 AT2G27140.1 40.252 159 74 5 9 155 10 159 2.04e-26 105
Msa0782760 AT5G20970.1 39.844 128 73 3 10 135 7 132 3.74e-19 85.9

Find 88 sgRNAs with CRISPR-Local

Find 228 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTTTCTCTTCTTTAGCTTT+TGG 0.144895 5_2:-66093665 Msa0782770:three_prime_UTR
GCTTTCTCTTCTATAGCTTT+TGG 0.148334 5_2:-66093899 Msa0782770:three_prime_UTR
GCTTTCTCTTCTTTAGCTTT+TGG 0.158375 5_2:-66093776 Msa0782770:three_prime_UTR
AGCAATAAACTTGGCATCTT+TGG 0.197304 5_2:-66094092 Msa0782770:three_prime_UTR
ATCTTATTGGTCGTGGGACT+TGG 0.235571 5_2:+66094244 Msa0782760:CDS
TTTGGTCTTGGAGCAGCTTC+TGG 0.245115 5_2:-66093605 Msa0782770:three_prime_UTR
TATTTATCCCATTCATGATA+TGG 0.253330 5_2:-66093106 None:intergenic
TTTGGTCTTGGAGCTTCTTC+TGG 0.254733 5_2:-66093827 Msa0782770:three_prime_UTR
TTTAGTCTTGGAGCTTCTTC+TGG 0.262302 5_2:-66093716 Msa0782770:three_prime_UTR
ACAAAGGCATAACTAATAAA+TGG 0.287057 5_2:+66093036 Msa0782760:exon
CTTAACATGCAGGATATAGA+AGG 0.298323 5_2:+66093372 Msa0782760:intron
GTCTTTCACCTATCAGCCTT+AGG 0.314056 5_2:-66093430 None:intergenic
TGTGATGGTTCCTTTGGTCT+TGG 0.318915 5_2:-66093617 Msa0782770:three_prime_UTR
CTTTATTGTAGAAATACATT+TGG 0.339189 5_2:+66094268 Msa0782760:CDS
TGTGGCGATTCCTTTGGTCT+TGG 0.352071 5_2:-66093839 Msa0782770:three_prime_UTR
GGATTTGTTGTTGTTTCCTT+AGG 0.356840 5_2:-66093581 None:intergenic
ATGTGTCATGCTAATCTTAT+TGG 0.363566 5_2:+66094231 Msa0782760:CDS
TCATAGACACGATCAGCTTC+TGG 0.364950 5_2:-66093073 None:intergenic
TGTAGATTTCACTCTCTCTA+AGG 0.366735 5_2:+66094132 Msa0782760:CDS
CTCTAAGGCTCTCTGTAATC+AGG 0.374231 5_2:+66094147 Msa0782760:CDS
TGCGATGGTTCCTTTAGTCT+TGG 0.384700 5_2:-66093728 Msa0782770:three_prime_UTR
AAATACATTTGGATCGTCTC+AGG 0.407520 5_2:+66094279 Msa0782760:CDS
ACAAAAGGTTGATGAGCAAA+AGG 0.416395 5_2:+66093637 Msa0782760:CDS
TGGATCGTCTCAGGGAGAAT+TGG 0.416829 5_2:+66094288 Msa0782760:CDS
ACAAAAGGCTGATGAGCAAA+AGG 0.421804 5_2:+66093859 Msa0782760:CDS
AAACCTGCCCTAAGGCTGAT+AGG 0.427961 5_2:+66093422 Msa0782760:CDS
TTGCCTCTTAGTAATTGAAA+TGG 0.441701 5_2:-66092991 None:intergenic
GCAAAACGTTGATGAGCAAA+AGG 0.446590 5_2:+66093748 Msa0782760:CDS
TCTTTCACCTATCAGCCTTA+GGG 0.449497 5_2:-66093429 None:intergenic
CATGCTAATCTTATTGGTCG+TGG 0.455442 5_2:+66094237 Msa0782760:CDS
ACTTTGAACCATATCATGAA+TGG 0.461998 5_2:+66093098 Msa0782760:CDS
CGTTTCACTGTCATGCTACC+AGG 0.466798 5_2:+66093136 Msa0782760:CDS
TCTTAAGAAGTGGAAGAGAT+TGG 0.472099 5_2:+66094204 Msa0782760:CDS
GCGGAGAAAGTGAGGACTAA+TGG 0.476427 5_2:+66094025 Msa0782760:CDS
TGCTAAACTTACTAATCCTA+AGG 0.484970 5_2:+66093565 Msa0782760:CDS
TCTTATTGGTCGTGGGACTT+GGG 0.489096 5_2:+66094245 Msa0782760:CDS
CGCCATCTGTTCTGAAAGGT+TGG 0.490011 5_2:-66093452 None:intergenic
CAGGATGAAAATGAAGCCCT+TGG 0.494161 5_2:+66094166 Msa0782760:CDS
ACCAAAGGAATCGCCACAAA+AGG 0.496416 5_2:+66093844 Msa0782760:CDS
TGACCATTTCAATTACTAAG+AGG 0.508482 5_2:+66092988 Msa0782760:five_prime_UTR
CTTTGAACCATATCATGAAT+GGG 0.508732 5_2:+66093099 Msa0782760:CDS
ATGCTAATCTTATTGGTCGT+GGG 0.509173 5_2:+66094238 Msa0782760:CDS
ATTGTATCATTCTCCTTCTG+TGG 0.509921 5_2:-66093962 Msa0782770:three_prime_UTR
ACCAAAGGAACCATCACAAA+AGG 0.514374 5_2:+66093622 Msa0782760:CDS
CAAATATCAGTACATCAGTA+AGG 0.527580 5_2:-66094334 Msa0782770:three_prime_UTR
TTATTGCTAGATGCAAAACC+AGG 0.528675 5_2:+66094107 Msa0782760:CDS
TTTGCATCTAGCAATAAACT+TGG 0.529793 5_2:-66094101 Msa0782770:three_prime_UTR
CAGTGTGACTGCGACATTTG+AGG 0.530897 5_2:+66093520 Msa0782760:CDS
AGAAGAGAAAGTAGAAACAA+AGG 0.532724 5_2:+66093676 Msa0782760:CDS
CATGAATGGGATAAATATGA+TGG 0.533667 5_2:+66093112 Msa0782760:CDS
AGTGTGACTGCGACATTTGA+GGG 0.535084 5_2:+66093521 Msa0782760:CDS
TCACCTATCAGCCTTAGGGC+AGG 0.538165 5_2:-66093425 None:intergenic
GTGTCATAATCAGAAGGAAT+GGG 0.540119 5_2:-66093494 None:intergenic
GCCCTTGGAAATTCGACCAC+AGG 0.540651 5_2:+66094181 Msa0782760:CDS
GAGAGTGAAATCTACAAGCC+TGG 0.545424 5_2:-66094125 Msa0782770:three_prime_UTR
GGTCTTGGAGCAGCTTCTGG+TGG 0.547053 5_2:-66093602 Msa0782770:three_prime_UTR
AGTCTTGGAGCTTCTTCTGG+TGG 0.547838 5_2:-66093713 Msa0782770:three_prime_UTR
CAAAAGGCTGATGAGCAAAA+GGG 0.548506 5_2:+66093860 Msa0782760:CDS
CAAAAGGTTGATGAGCAAAA+GGG 0.551402 5_2:+66093638 Msa0782760:CDS
AGAAGAAGCTCCAAGACTAA+AGG 0.551790 5_2:+66093718 Msa0782760:CDS
GACCTGTGGTCGAATTTCCA+AGG 0.558465 5_2:-66094183 Msa0782770:three_prime_UTR
AGAAGAGAAAGCAGAAACAA+AGG 0.563791 5_2:+66093787 Msa0782760:CDS
GACCAACCTTTCAGAACAGA+TGG 0.572272 5_2:+66093450 Msa0782760:CDS
GTATCATTCTCCTTCTGTGG+TGG 0.573843 5_2:-66093959 Msa0782770:three_prime_UTR
CTGGTAGCATGACAGTGAAA+CGG 0.576811 5_2:-66093135 None:intergenic
CATTCCTTCTGATTATGACA+CGG 0.581427 5_2:+66093496 Msa0782760:CDS
TGACCTCAAAACCTGCCCTA+AGG 0.581927 5_2:+66093414 Msa0782760:CDS
CGTGTCATAATCAGAAGGAA+TGG 0.584263 5_2:-66093495 None:intergenic
TTAACATGCAGGATATAGAA+GGG 0.585688 5_2:+66093373 Msa0782760:intron
ATATAGAAGGGATCAAATGA+AGG 0.587427 5_2:+66093385 Msa0782760:CDS
CAAAACGTTGATGAGCAAAA+GGG 0.587682 5_2:+66093749 Msa0782760:CDS
AATACATTTGGATCGTCTCA+GGG 0.587906 5_2:+66094280 Msa0782760:CDS
TGAGCAAAAGGGTGCTCAAG+AGG 0.590093 5_2:+66093871 Msa0782760:CDS
ACCTGTGGTCGAATTTCCAA+GGG 0.591551 5_2:-66094182 Msa0782770:three_prime_UTR
TCAAAAGACACCACCACAGA+AGG 0.591734 5_2:+66093949 Msa0782760:CDS
CGACCACAGGTCTTAAGAAG+TGG 0.593414 5_2:+66094194 Msa0782760:CDS
ACAAAGTGGCGGAGAAAGTG+AGG 0.597287 5_2:+66094017 Msa0782760:CDS
GGTCTTGGAGCTTCTTCTGG+CGG 0.600665 5_2:-66093824 Msa0782770:three_prime_UTR
GAGCAAAAGGGTGCTCAAGA+GGG 0.603253 5_2:+66093872 Msa0782760:CDS
CTTCCACTTCTTAAGACCTG+TGG 0.604468 5_2:-66094197 Msa0782770:three_prime_UTR
ACGACGCCATCTGTTCTGAA+AGG 0.621474 5_2:-66093456 None:intergenic
AGAAGAAGCTCCAAGACCAA+AGG 0.632632 5_2:+66093829 Msa0782760:CDS
AGAAGCTAGCATAGACAAAG+TGG 0.640905 5_2:+66094003 Msa0782760:CDS
AGAAGCTGCTCCAAGACCAA+AGG 0.649599 5_2:+66093607 Msa0782760:CDS
CTGTCCGTGTCATAATCAGA+AGG 0.662264 5_2:-66093500 None:intergenic
AGCTAGCATAGACAAAGTGG+CGG 0.665250 5_2:+66094006 Msa0782760:CDS
TAATTGAAATGGTCATGACA+TGG 0.727826 5_2:-66092980 None:intergenic
GTGACTGCGACATTTGAGGG+TGG 0.759268 5_2:+66093524 Msa0782760:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GTAAAAAAATGTATATTATT+TGG - chr5_2:66093221-66093240 None:intergenic 10.0%
!! GTAAAAAAATGTATATTATT+TGG - chr5_2:66093221-66093240 None:intergenic 10.0%
!!! TAATTCAAGTTTTTATATCT+TGG + chr5_2:66093166-66093185 Msa0782760:intron 15.0%
!!! ATATACATTTTTTTACTGTA+AGG + chr5_2:66093225-66093244 Msa0782760:intron 15.0%
!! TCAAATTAATGAAAAACAAA+GGG - chr5_2:66093296-66093315 None:intergenic 15.0%
!! TTCAAATTAATGAAAAACAA+AGG - chr5_2:66093297-66093316 None:intergenic 15.0%
!!! TTTGTTTTTCATTAATTTGA+AGG + chr5_2:66093296-66093315 Msa0782760:intron 15.0%
!!! AGTATATAGTTTGTATTTTT+TGG - chr5_2:66093350-66093369 None:intergenic 15.0%
!!! TAATTCAAGTTTTTATATCT+TGG + chr5_2:66093166-66093185 Msa0782760:intron 15.0%
!!! ATATACATTTTTTTACTGTA+AGG + chr5_2:66093225-66093244 Msa0782760:intron 15.0%
!! TCAAATTAATGAAAAACAAA+GGG - chr5_2:66093296-66093315 None:intergenic 15.0%
!! TTCAAATTAATGAAAAACAA+AGG - chr5_2:66093297-66093316 None:intergenic 15.0%
!!! TTTGTTTTTCATTAATTTGA+AGG + chr5_2:66093296-66093315 Msa0782760:intron 15.0%
!!! AGTATATAGTTTGTATTTTT+TGG - chr5_2:66093350-66093369 None:intergenic 15.0%
!! ATAAAAGCAAAAAGAAACAA+AGG + chr5_2:66093020-66093039 Msa0782760:five_prime_UTR 20.0%
!! CTTTATTGTAGAAATACATT+TGG + chr5_2:66094268-66094287 Msa0782760:CDS 20.0%
!! ATAAAAGCAAAAAGAAACAA+AGG + chr5_2:66093020-66093039 Msa0782760:five_prime_UTR 20.0%
!! CTTTATTGTAGAAATACATT+TGG + chr5_2:66094268-66094287 Msa0782760:CDS 20.0%
!! ACAAAGGCATAACTAATAAA+TGG + chr5_2:66093036-66093055 Msa0782760:exon 25.0%
! TATTTATCCCATTCATGATA+TGG - chr5_2:66093109-66093128 None:intergenic 25.0%
! AACTTGAATTAACATTTACC+TGG - chr5_2:66093157-66093176 None:intergenic 25.0%
! TAGTTGTCTTATGAGTTATT+TGG - chr5_2:66093256-66093275 None:intergenic 25.0%
!! AAACAAAGGGCTTTTAAATA+TGG - chr5_2:66093283-66093302 None:intergenic 25.0%
! TATATACTTTCTTAACATGC+AGG + chr5_2:66093362-66093381 Msa0782760:intron 25.0%
!! ACAAAGGCATAACTAATAAA+TGG + chr5_2:66093036-66093055 Msa0782760:exon 25.0%
! TATTTATCCCATTCATGATA+TGG - chr5_2:66093109-66093128 None:intergenic 25.0%
! AACTTGAATTAACATTTACC+TGG - chr5_2:66093157-66093176 None:intergenic 25.0%
! TAGTTGTCTTATGAGTTATT+TGG - chr5_2:66093256-66093275 None:intergenic 25.0%
!! AAACAAAGGGCTTTTAAATA+TGG - chr5_2:66093283-66093302 None:intergenic 25.0%
! TATATACTTTCTTAACATGC+AGG + chr5_2:66093362-66093381 Msa0782760:intron 25.0%
TAATTGAAATGGTCATGACA+TGG - chr5_2:66092983-66093002 None:intergenic 30.0%
TGACCATTTCAATTACTAAG+AGG + chr5_2:66092988-66093007 Msa0782760:five_prime_UTR 30.0%
TTGCCTCTTAGTAATTGAAA+TGG - chr5_2:66092994-66093013 None:intergenic 30.0%
ACTTTGAACCATATCATGAA+TGG + chr5_2:66093098-66093117 Msa0782760:CDS 30.0%
CTTTGAACCATATCATGAAT+GGG + chr5_2:66093099-66093118 Msa0782760:CDS 30.0%
CATGAATGGGATAAATATGA+TGG + chr5_2:66093112-66093131 Msa0782760:CDS 30.0%
TTAACATGCAGGATATAGAA+GGG + chr5_2:66093373-66093392 Msa0782760:intron 30.0%
ATATAGAAGGGATCAAATGA+AGG + chr5_2:66093385-66093404 Msa0782760:CDS 30.0%
TGCTAAACTTACTAATCCTA+AGG + chr5_2:66093565-66093584 Msa0782760:CDS 30.0%
!!! TTTCTCTTCTTTAGCTTTTG+GGG - chr5_2:66093666-66093685 Msa0782770:three_prime_UTR 30.0%
!!! CTTTCTCTTCTTTAGCTTTT+GGG - chr5_2:66093667-66093686 Msa0782770:three_prime_UTR 30.0%
!!! ACTTTCTCTTCTTTAGCTTT+TGG - chr5_2:66093668-66093687 Msa0782770:three_prime_UTR 30.0%
AGAAGAGAAAGTAGAAACAA+AGG + chr5_2:66093676-66093695 Msa0782760:CDS 30.0%
!!! TTTCTCTTCTTTAGCTTTTG+GGG - chr5_2:66093666-66093685 Msa0782770:three_prime_UTR 30.0%
!!! CTTTCTCTTCTTTAGCTTTT+GGG - chr5_2:66093667-66093686 Msa0782770:three_prime_UTR 30.0%
! TTTCTCTTCTATAGCTTTTG+GGG - chr5_2:66093900-66093919 Msa0782770:three_prime_UTR 30.0%
! CTTTCTCTTCTATAGCTTTT+GGG - chr5_2:66093901-66093920 Msa0782770:three_prime_UTR 30.0%
TTTGCATCTAGCAATAAACT+TGG - chr5_2:66094104-66094123 Msa0782770:three_prime_UTR 30.0%
ATGTGTCATGCTAATCTTAT+TGG + chr5_2:66094231-66094250 Msa0782760:CDS 30.0%
TAATTGAAATGGTCATGACA+TGG - chr5_2:66092983-66093002 None:intergenic 30.0%
TGACCATTTCAATTACTAAG+AGG + chr5_2:66092988-66093007 Msa0782760:five_prime_UTR 30.0%
TTGCCTCTTAGTAATTGAAA+TGG - chr5_2:66092994-66093013 None:intergenic 30.0%
ACTTTGAACCATATCATGAA+TGG + chr5_2:66093098-66093117 Msa0782760:CDS 30.0%
CTTTGAACCATATCATGAAT+GGG + chr5_2:66093099-66093118 Msa0782760:CDS 30.0%
CATGAATGGGATAAATATGA+TGG + chr5_2:66093112-66093131 Msa0782760:CDS 30.0%
TTAACATGCAGGATATAGAA+GGG + chr5_2:66093373-66093392 Msa0782760:intron 30.0%
ATATAGAAGGGATCAAATGA+AGG + chr5_2:66093385-66093404 Msa0782760:CDS 30.0%
TGCTAAACTTACTAATCCTA+AGG + chr5_2:66093565-66093584 Msa0782760:CDS 30.0%
!!! TTTCTCTTCTTTAGCTTTTG+GGG - chr5_2:66093666-66093685 Msa0782770:three_prime_UTR 30.0%
!!! CTTTCTCTTCTTTAGCTTTT+GGG - chr5_2:66093667-66093686 Msa0782770:three_prime_UTR 30.0%
!!! ACTTTCTCTTCTTTAGCTTT+TGG - chr5_2:66093668-66093687 Msa0782770:three_prime_UTR 30.0%
AGAAGAGAAAGTAGAAACAA+AGG + chr5_2:66093676-66093695 Msa0782760:CDS 30.0%
!!! TTTCTCTTCTTTAGCTTTTG+GGG - chr5_2:66093666-66093685 Msa0782770:three_prime_UTR 30.0%
!!! CTTTCTCTTCTTTAGCTTTT+GGG - chr5_2:66093667-66093686 Msa0782770:three_prime_UTR 30.0%
! TTTCTCTTCTATAGCTTTTG+GGG - chr5_2:66093900-66093919 Msa0782770:three_prime_UTR 30.0%
! CTTTCTCTTCTATAGCTTTT+GGG - chr5_2:66093901-66093920 Msa0782770:three_prime_UTR 30.0%
TTTGCATCTAGCAATAAACT+TGG - chr5_2:66094104-66094123 Msa0782770:three_prime_UTR 30.0%
ATGTGTCATGCTAATCTTAT+TGG + chr5_2:66094231-66094250 Msa0782760:CDS 30.0%
CTTAACATGCAGGATATAGA+AGG + chr5_2:66093372-66093391 Msa0782760:intron 35.0%
GTGTCATAATCAGAAGGAAT+GGG - chr5_2:66093497-66093516 None:intergenic 35.0%
CATTCCTTCTGATTATGACA+CGG + chr5_2:66093496-66093515 Msa0782760:CDS 35.0%
!! GGATTTGTTGTTGTTTCCTT+AGG - chr5_2:66093584-66093603 Msa0782770:three_prime_UTR 35.0%
!! ACAAAAGGTTGATGAGCAAA+AGG + chr5_2:66093637-66093656 Msa0782760:CDS 35.0%
!! CAAAAGGTTGATGAGCAAAA+GGG + chr5_2:66093638-66093657 Msa0782760:CDS 35.0%
! CAAAACGTTGATGAGCAAAA+GGG + chr5_2:66093749-66093768 Msa0782760:CDS 35.0%
!!! GCTTTCTCTTCTTTAGCTTT+TGG - chr5_2:66093779-66093798 Msa0782770:three_prime_UTR 35.0%
AGAAGAGAAAGCAGAAACAA+AGG + chr5_2:66093787-66093806 Msa0782760:CDS 35.0%
! GCTTTCTCTTCTATAGCTTT+TGG - chr5_2:66093902-66093921 Msa0782770:three_prime_UTR 35.0%
ATTGTATCATTCTCCTTCTG+TGG - chr5_2:66093965-66093984 Msa0782770:three_prime_UTR 35.0%
!!! AACTTGGCATCTTTGGTTTT+AGG - chr5_2:66094088-66094107 Msa0782770:three_prime_UTR 35.0%
AGCAATAAACTTGGCATCTT+TGG - chr5_2:66094095-66094114 Msa0782770:three_prime_UTR 35.0%
TTATTGCTAGATGCAAAACC+AGG + chr5_2:66094107-66094126 Msa0782760:CDS 35.0%
TGTAGATTTCACTCTCTCTA+AGG + chr5_2:66094132-66094151 Msa0782760:CDS 35.0%
TCTTAAGAAGTGGAAGAGAT+TGG + chr5_2:66094204-66094223 Msa0782760:CDS 35.0%
ATGCTAATCTTATTGGTCGT+GGG + chr5_2:66094238-66094257 Msa0782760:CDS 35.0%
AAATACATTTGGATCGTCTC+AGG + chr5_2:66094279-66094298 Msa0782760:CDS 35.0%
AATACATTTGGATCGTCTCA+GGG + chr5_2:66094280-66094299 Msa0782760:CDS 35.0%
CTTAACATGCAGGATATAGA+AGG + chr5_2:66093372-66093391 Msa0782760:intron 35.0%
GTGTCATAATCAGAAGGAAT+GGG - chr5_2:66093497-66093516 None:intergenic 35.0%
CATTCCTTCTGATTATGACA+CGG + chr5_2:66093496-66093515 Msa0782760:CDS 35.0%
!! GGATTTGTTGTTGTTTCCTT+AGG - chr5_2:66093584-66093603 Msa0782770:three_prime_UTR 35.0%
!! ACAAAAGGTTGATGAGCAAA+AGG + chr5_2:66093637-66093656 Msa0782760:CDS 35.0%
!! CAAAAGGTTGATGAGCAAAA+GGG + chr5_2:66093638-66093657 Msa0782760:CDS 35.0%
! CAAAACGTTGATGAGCAAAA+GGG + chr5_2:66093749-66093768 Msa0782760:CDS 35.0%
!!! GCTTTCTCTTCTTTAGCTTT+TGG - chr5_2:66093779-66093798 Msa0782770:three_prime_UTR 35.0%
AGAAGAGAAAGCAGAAACAA+AGG + chr5_2:66093787-66093806 Msa0782760:CDS 35.0%
! GCTTTCTCTTCTATAGCTTT+TGG - chr5_2:66093902-66093921 Msa0782770:three_prime_UTR 35.0%
ATTGTATCATTCTCCTTCTG+TGG - chr5_2:66093965-66093984 Msa0782770:three_prime_UTR 35.0%
!!! AACTTGGCATCTTTGGTTTT+AGG - chr5_2:66094088-66094107 Msa0782770:three_prime_UTR 35.0%
AGCAATAAACTTGGCATCTT+TGG - chr5_2:66094095-66094114 Msa0782770:three_prime_UTR 35.0%
TTATTGCTAGATGCAAAACC+AGG + chr5_2:66094107-66094126 Msa0782760:CDS 35.0%
TGTAGATTTCACTCTCTCTA+AGG + chr5_2:66094132-66094151 Msa0782760:CDS 35.0%
TCTTAAGAAGTGGAAGAGAT+TGG + chr5_2:66094204-66094223 Msa0782760:CDS 35.0%
ATGCTAATCTTATTGGTCGT+GGG + chr5_2:66094238-66094257 Msa0782760:CDS 35.0%
AAATACATTTGGATCGTCTC+AGG + chr5_2:66094279-66094298 Msa0782760:CDS 35.0%
AATACATTTGGATCGTCTCA+GGG + chr5_2:66094280-66094299 Msa0782760:CDS 35.0%
TCTTTCACCTATCAGCCTTA+GGG - chr5_2:66093432-66093451 None:intergenic 40.0%
CGTGTCATAATCAGAAGGAA+TGG - chr5_2:66093498-66093517 None:intergenic 40.0%
ACCAAAGGAACCATCACAAA+AGG + chr5_2:66093622-66093641 Msa0782760:CDS 40.0%
! ACCTTTTGTGATGGTTCCTT+TGG - chr5_2:66093626-66093645 Msa0782770:three_prime_UTR 40.0%
! TGCTCATCAACCTTTTGTGA+TGG - chr5_2:66093635-66093654 Msa0782770:three_prime_UTR 40.0%
!! TTTAGTCTTGGAGCTTCTTC+TGG - chr5_2:66093719-66093738 Msa0782770:three_prime_UTR 40.0%
AGAAGAAGCTCCAAGACTAA+AGG + chr5_2:66093718-66093737 Msa0782760:CDS 40.0%
! GCAAAACGTTGATGAGCAAA+AGG + chr5_2:66093748-66093767 Msa0782760:CDS 40.0%
! TTTTGCTCATCAGCCTTTTG+TGG - chr5_2:66093860-66093879 Msa0782770:three_prime_UTR 40.0%
ACAAAAGGCTGATGAGCAAA+AGG + chr5_2:66093859-66093878 Msa0782760:CDS 40.0%
CAAAAGGCTGATGAGCAAAA+GGG + chr5_2:66093860-66093879 Msa0782760:CDS 40.0%
! AGCTTCTGTTCTGCTCTTTT+CGG - chr5_2:66093990-66094009 Msa0782770:three_prime_UTR 40.0%
AGAAGCTAGCATAGACAAAG+TGG + chr5_2:66094003-66094022 Msa0782760:CDS 40.0%
CATGCTAATCTTATTGGTCG+TGG + chr5_2:66094237-66094256 Msa0782760:CDS 40.0%
TCTTTCACCTATCAGCCTTA+GGG - chr5_2:66093432-66093451 None:intergenic 40.0%
CGTGTCATAATCAGAAGGAA+TGG - chr5_2:66093498-66093517 None:intergenic 40.0%
ACCAAAGGAACCATCACAAA+AGG + chr5_2:66093622-66093641 Msa0782760:CDS 40.0%
! ACCTTTTGTGATGGTTCCTT+TGG - chr5_2:66093626-66093645 Msa0782770:three_prime_UTR 40.0%
! TGCTCATCAACCTTTTGTGA+TGG - chr5_2:66093635-66093654 Msa0782770:three_prime_UTR 40.0%
!! TTTAGTCTTGGAGCTTCTTC+TGG - chr5_2:66093719-66093738 Msa0782770:three_prime_UTR 40.0%
AGAAGAAGCTCCAAGACTAA+AGG + chr5_2:66093718-66093737 Msa0782760:CDS 40.0%
! GCAAAACGTTGATGAGCAAA+AGG + chr5_2:66093748-66093767 Msa0782760:CDS 40.0%
! TTTTGCTCATCAGCCTTTTG+TGG - chr5_2:66093860-66093879 Msa0782770:three_prime_UTR 40.0%
ACAAAAGGCTGATGAGCAAA+AGG + chr5_2:66093859-66093878 Msa0782760:CDS 40.0%
CAAAAGGCTGATGAGCAAAA+GGG + chr5_2:66093860-66093879 Msa0782760:CDS 40.0%
! AGCTTCTGTTCTGCTCTTTT+CGG - chr5_2:66093990-66094009 Msa0782770:three_prime_UTR 40.0%
AGAAGCTAGCATAGACAAAG+TGG + chr5_2:66094003-66094022 Msa0782760:CDS 40.0%
CATGCTAATCTTATTGGTCG+TGG + chr5_2:66094237-66094256 Msa0782760:CDS 40.0%
TCATAGACACGATCAGCTTC+TGG - chr5_2:66093076-66093095 None:intergenic 45.0%
CTGGTAGCATGACAGTGAAA+CGG - chr5_2:66093138-66093157 None:intergenic 45.0%
GTCTTTCACCTATCAGCCTT+AGG - chr5_2:66093433-66093452 None:intergenic 45.0%
GACCAACCTTTCAGAACAGA+TGG + chr5_2:66093450-66093469 Msa0782760:CDS 45.0%
CTGTCCGTGTCATAATCAGA+AGG - chr5_2:66093503-66093522 None:intergenic 45.0%
AGTGTGACTGCGACATTTGA+GGG + chr5_2:66093521-66093540 Msa0782760:CDS 45.0%
TGTGATGGTTCCTTTGGTCT+TGG - chr5_2:66093620-66093639 Msa0782770:three_prime_UTR 45.0%
TGCGATGGTTCCTTTAGTCT+TGG - chr5_2:66093731-66093750 Msa0782770:three_prime_UTR 45.0%
! TGCTCATCAACGTTTTGCGA+TGG - chr5_2:66093746-66093765 Msa0782770:three_prime_UTR 45.0%
!! TTTGGTCTTGGAGCTTCTTC+TGG - chr5_2:66093830-66093849 Msa0782770:three_prime_UTR 45.0%
AGAAGAAGCTCCAAGACCAA+AGG + chr5_2:66093829-66093848 Msa0782760:CDS 45.0%
ACCAAAGGAATCGCCACAAA+AGG + chr5_2:66093844-66093863 Msa0782760:CDS 45.0%
TCAAAAGACACCACCACAGA+AGG + chr5_2:66093949-66093968 Msa0782760:CDS 45.0%
GTATCATTCTCCTTCTGTGG+TGG - chr5_2:66093962-66093981 Msa0782770:three_prime_UTR 45.0%
AGCTAGCATAGACAAAGTGG+CGG + chr5_2:66094006-66094025 Msa0782760:CDS 45.0%
GAGAGTGAAATCTACAAGCC+TGG - chr5_2:66094128-66094147 Msa0782770:three_prime_UTR 45.0%
!! CTCTAAGGCTCTCTGTAATC+AGG + chr5_2:66094147-66094166 Msa0782760:CDS 45.0%
CAGGATGAAAATGAAGCCCT+TGG + chr5_2:66094166-66094185 Msa0782760:CDS 45.0%
ACCTGTGGTCGAATTTCCAA+GGG - chr5_2:66094185-66094204 Msa0782770:three_prime_UTR 45.0%
CTTCCACTTCTTAAGACCTG+TGG - chr5_2:66094200-66094219 Msa0782770:three_prime_UTR 45.0%
ATCTTATTGGTCGTGGGACT+TGG + chr5_2:66094244-66094263 Msa0782760:CDS 45.0%
TCTTATTGGTCGTGGGACTT+GGG + chr5_2:66094245-66094264 Msa0782760:CDS 45.0%
TCATAGACACGATCAGCTTC+TGG - chr5_2:66093076-66093095 None:intergenic 45.0%
CTGGTAGCATGACAGTGAAA+CGG - chr5_2:66093138-66093157 None:intergenic 45.0%
GTCTTTCACCTATCAGCCTT+AGG - chr5_2:66093433-66093452 None:intergenic 45.0%
GACCAACCTTTCAGAACAGA+TGG + chr5_2:66093450-66093469 Msa0782760:CDS 45.0%
CTGTCCGTGTCATAATCAGA+AGG - chr5_2:66093503-66093522 None:intergenic 45.0%
AGTGTGACTGCGACATTTGA+GGG + chr5_2:66093521-66093540 Msa0782760:CDS 45.0%
TGTGATGGTTCCTTTGGTCT+TGG - chr5_2:66093620-66093639 Msa0782770:three_prime_UTR 45.0%
TGCGATGGTTCCTTTAGTCT+TGG - chr5_2:66093731-66093750 Msa0782770:three_prime_UTR 45.0%
! TGCTCATCAACGTTTTGCGA+TGG - chr5_2:66093746-66093765 Msa0782770:three_prime_UTR 45.0%
!! TTTGGTCTTGGAGCTTCTTC+TGG - chr5_2:66093830-66093849 Msa0782770:three_prime_UTR 45.0%
AGAAGAAGCTCCAAGACCAA+AGG + chr5_2:66093829-66093848 Msa0782760:CDS 45.0%
ACCAAAGGAATCGCCACAAA+AGG + chr5_2:66093844-66093863 Msa0782760:CDS 45.0%
TCAAAAGACACCACCACAGA+AGG + chr5_2:66093949-66093968 Msa0782760:CDS 45.0%
GTATCATTCTCCTTCTGTGG+TGG - chr5_2:66093962-66093981 Msa0782770:three_prime_UTR 45.0%
AGCTAGCATAGACAAAGTGG+CGG + chr5_2:66094006-66094025 Msa0782760:CDS 45.0%
GAGAGTGAAATCTACAAGCC+TGG - chr5_2:66094128-66094147 Msa0782770:three_prime_UTR 45.0%
!! CTCTAAGGCTCTCTGTAATC+AGG + chr5_2:66094147-66094166 Msa0782760:CDS 45.0%
CAGGATGAAAATGAAGCCCT+TGG + chr5_2:66094166-66094185 Msa0782760:CDS 45.0%
ACCTGTGGTCGAATTTCCAA+GGG - chr5_2:66094185-66094204 Msa0782770:three_prime_UTR 45.0%
CTTCCACTTCTTAAGACCTG+TGG - chr5_2:66094200-66094219 Msa0782770:three_prime_UTR 45.0%
ATCTTATTGGTCGTGGGACT+TGG + chr5_2:66094244-66094263 Msa0782760:CDS 45.0%
TCTTATTGGTCGTGGGACTT+GGG + chr5_2:66094245-66094264 Msa0782760:CDS 45.0%
!! TAAAAAAATGTATATTATTT+GGG - chr5_2:66093220-66093239 None:intergenic 5.0%
!! TAAAAAAATGTATATTATTT+GGG - chr5_2:66093220-66093239 None:intergenic 5.0%
CGTTTCACTGTCATGCTACC+AGG + chr5_2:66093136-66093155 Msa0782760:CDS 50.0%
TGACCTCAAAACCTGCCCTA+AGG + chr5_2:66093414-66093433 Msa0782760:CDS 50.0%
AAACCTGCCCTAAGGCTGAT+AGG + chr5_2:66093422-66093441 Msa0782760:CDS 50.0%
!! CGCCATCTGTTCTGAAAGGT+TGG - chr5_2:66093455-66093474 None:intergenic 50.0%
! ACGACGCCATCTGTTCTGAA+AGG - chr5_2:66093459-66093478 None:intergenic 50.0%
CAGTGTGACTGCGACATTTG+AGG + chr5_2:66093520-66093539 Msa0782760:CDS 50.0%
!! TTTGGTCTTGGAGCAGCTTC+TGG - chr5_2:66093608-66093627 Msa0782770:three_prime_UTR 50.0%
AGAAGCTGCTCCAAGACCAA+AGG + chr5_2:66093607-66093626 Msa0782760:CDS 50.0%
AGTCTTGGAGCTTCTTCTGG+TGG - chr5_2:66093716-66093735 Msa0782770:three_prime_UTR 50.0%
TGTGGCGATTCCTTTGGTCT+TGG - chr5_2:66093842-66093861 Msa0782770:three_prime_UTR 50.0%
! GCCTTTTGTGGCGATTCCTT+TGG - chr5_2:66093848-66093867 Msa0782770:three_prime_UTR 50.0%
!! TGAGCAAAAGGGTGCTCAAG+AGG + chr5_2:66093871-66093890 Msa0782760:CDS 50.0%
!! GAGCAAAAGGGTGCTCAAGA+GGG + chr5_2:66093872-66093891 Msa0782760:CDS 50.0%
! ACAAAGTGGCGGAGAAAGTG+AGG + chr5_2:66094017-66094036 Msa0782760:CDS 50.0%
GCGGAGAAAGTGAGGACTAA+TGG + chr5_2:66094025-66094044 Msa0782760:CDS 50.0%
GACCTGTGGTCGAATTTCCA+AGG - chr5_2:66094186-66094205 Msa0782770:three_prime_UTR 50.0%
CGACCACAGGTCTTAAGAAG+TGG + chr5_2:66094194-66094213 Msa0782760:CDS 50.0%
TGGATCGTCTCAGGGAGAAT+TGG + chr5_2:66094288-66094307 Msa0782760:CDS 50.0%
CGTTTCACTGTCATGCTACC+AGG + chr5_2:66093136-66093155 Msa0782760:CDS 50.0%
TGACCTCAAAACCTGCCCTA+AGG + chr5_2:66093414-66093433 Msa0782760:CDS 50.0%
AAACCTGCCCTAAGGCTGAT+AGG + chr5_2:66093422-66093441 Msa0782760:CDS 50.0%
!! CGCCATCTGTTCTGAAAGGT+TGG - chr5_2:66093455-66093474 None:intergenic 50.0%
! ACGACGCCATCTGTTCTGAA+AGG - chr5_2:66093459-66093478 None:intergenic 50.0%
CAGTGTGACTGCGACATTTG+AGG + chr5_2:66093520-66093539 Msa0782760:CDS 50.0%
!! TTTGGTCTTGGAGCAGCTTC+TGG - chr5_2:66093608-66093627 Msa0782770:three_prime_UTR 50.0%
AGAAGCTGCTCCAAGACCAA+AGG + chr5_2:66093607-66093626 Msa0782760:CDS 50.0%
AGTCTTGGAGCTTCTTCTGG+TGG - chr5_2:66093716-66093735 Msa0782770:three_prime_UTR 50.0%
TGTGGCGATTCCTTTGGTCT+TGG - chr5_2:66093842-66093861 Msa0782770:three_prime_UTR 50.0%
! GCCTTTTGTGGCGATTCCTT+TGG - chr5_2:66093848-66093867 Msa0782770:three_prime_UTR 50.0%
!! TGAGCAAAAGGGTGCTCAAG+AGG + chr5_2:66093871-66093890 Msa0782760:CDS 50.0%
!! GAGCAAAAGGGTGCTCAAGA+GGG + chr5_2:66093872-66093891 Msa0782760:CDS 50.0%
! ACAAAGTGGCGGAGAAAGTG+AGG + chr5_2:66094017-66094036 Msa0782760:CDS 50.0%
GCGGAGAAAGTGAGGACTAA+TGG + chr5_2:66094025-66094044 Msa0782760:CDS 50.0%
GACCTGTGGTCGAATTTCCA+AGG - chr5_2:66094186-66094205 Msa0782770:three_prime_UTR 50.0%
CGACCACAGGTCTTAAGAAG+TGG + chr5_2:66094194-66094213 Msa0782760:CDS 50.0%
TGGATCGTCTCAGGGAGAAT+TGG + chr5_2:66094288-66094307 Msa0782760:CDS 50.0%
! CAGCCTTAGGGCAGGTTTTG+AGG - chr5_2:66093420-66093439 None:intergenic 55.0%
TCACCTATCAGCCTTAGGGC+AGG - chr5_2:66093428-66093447 None:intergenic 55.0%
GTGACTGCGACATTTGAGGG+TGG + chr5_2:66093524-66093543 Msa0782760:CDS 55.0%
GGTCTTGGAGCTTCTTCTGG+CGG - chr5_2:66093827-66093846 Msa0782770:three_prime_UTR 55.0%
GCCCTTGGAAATTCGACCAC+AGG + chr5_2:66094181-66094200 Msa0782760:CDS 55.0%
! CAGCCTTAGGGCAGGTTTTG+AGG - chr5_2:66093420-66093439 None:intergenic 55.0%
TCACCTATCAGCCTTAGGGC+AGG - chr5_2:66093428-66093447 None:intergenic 55.0%
GTGACTGCGACATTTGAGGG+TGG + chr5_2:66093524-66093543 Msa0782760:CDS 55.0%
GGTCTTGGAGCTTCTTCTGG+CGG - chr5_2:66093827-66093846 Msa0782770:three_prime_UTR 55.0%
GCCCTTGGAAATTCGACCAC+AGG + chr5_2:66094181-66094200 Msa0782760:CDS 55.0%
GGTCTTGGAGCAGCTTCTGG+TGG - chr5_2:66093605-66093624 Msa0782770:three_prime_UTR 60.0%
GGTCTTGGAGCAGCTTCTGG+TGG - chr5_2:66093605-66093624 Msa0782770:three_prime_UTR 60.0%
Chromosome Type Strat End Strand Name
chr5_2 gene 66092913 66094342 66092913 ID=Msa0782760;Name=Msa0782760
chr5_2 mRNA 66092913 66094342 66092913 ID=Msa0782760-mRNA-1;Parent=Msa0782760;Name=Msa0782760-mRNA-1;_AED=0.00;_eAED=0.00;_QI=142|1|0.5|1|1|1|2|0|353
chr5_2 exon 66092913 66093157 66092913 ID=Msa0782760-mRNA-1:exon:19894;Parent=Msa0782760-mRNA-1
chr5_2 exon 66093384 66094342 66093384 ID=Msa0782760-mRNA-1:exon:19895;Parent=Msa0782760-mRNA-1
chr5_2 five_prime_UTR 66092913 66093054 66092913 ID=Msa0782760-mRNA-1:five_prime_utr;Parent=Msa0782760-mRNA-1
chr5_2 CDS 66093055 66093157 66093055 ID=Msa0782760-mRNA-1:cds;Parent=Msa0782760-mRNA-1
chr5_2 CDS 66093384 66094342 66093384 ID=Msa0782760-mRNA-1:cds;Parent=Msa0782760-mRNA-1
Gene Sequence

>Msa0782760

ATGGATACAAAGACACAGCCAGAAGCTGATCGTGTCTATGAAGACTTTGAACCATATCATGAATGGGATAAATATGATGGCCGTTTCACTGTCATGCTACCAGGATATAGAAGGGATCAAATGAAGGTTCAAGTGACCTCAAAACCTGCCCTAAGGCTGATAGGTGAAAGACCAACCTTTCAGAACAGATGGCGTCGTTTTAAATTAGAATTTCCCATTCCTTCTGATTATGACACGGACAGTGTGACTGCGACATTTGAGGGTGGCAAGCTATCTGTCAAATTTGCTAAACTTACTAATCCTAAGGAAACAACAACAAATCCACCAGAAGCTGCTCCAAGACCAAAGGAACCATCACAAAAGGTTGATGAGCAAAAGGGTACCCCAAAAGCTAAAGAAGAGAAAGTAGAAACAAAGGAAACAACAACAAATCCACCAGAAGAAGCTCCAAGACTAAAGGAACCATCGCAAAACGTTGATGAGCAAAAGGGTACCCCAAAAGCTAAAGAAGAGAAAGCAGAAACAAAGGAAACAACAACAAATCCGCCAGAAGAAGCTCCAAGACCAAAGGAATCGCCACAAAAGGCTGATGAGCAAAAGGGTGCTCAAGAGGGTACCCCAAAAGCTATAGAAGAGAAAGCAAAAACAAAGACTAACGATGTCTCTGATCAAAAGACACCACCACAGAAGGAGAATGATACAATAACCGAAAAGAGCAGAACAGAAGCTAGCATAGACAAAGTGGCGGAGAAAGTGAGGACTAATGGCTCAGCTGAGACAATAGACGCAGCAACATCTAATTCTCCTAAAACCAAAGATGCCAAGTTTATTGCTAGATGCAAAACCAGGCTTGTAGATTTCACTCTCTCTAAGGCTCTCTGTAATCAGGATGAAAATGAAGCCCTTGGAAATTCGACCACAGGTCTTAAGAAGTGGAAGAGATTGGTTGCATGTGTCATGCTAATCTTATTGGTCGTGGGACTTGGGCTTTATTGTAGAAATACATTTGGATCGTCTCAGGGAGAATTGGACTTCGAAGAGCTGTTATTATTTCCTTACTGA
Protein sequence

>Msa0782760

MDTKTQPEADRVYEDFEPYHEWDKYDGRFTVMLPGYRRDQMKVQVTSKPALRLIGERPTFQNRWRRFKLEFPIPSDYDTDSVTATFEGGKLSVKFAKLTNPKETTTNPPEAAPRPKEPSQKVDEQKGTPKAKEEKVETKETTTNPPEEAPRLKEPSQNVDEQKGTPKAKEEKAETKETTTNPPEEAPRPKESPQKADEQKGAQEGTPKAIEEKAKTKTNDVSDQKTPPQKENDTITEKSRTEASIDKVAEKVRTNGSAETIDAATSNSPKTKDAKFIARCKTRLVDFTLSKALCNQDENEALGNSTTGLKKWKRLVACVMLILLVVGLGLYCRNTFGSSQGELDFEELLLFPY*